Blotting知识介绍

相关专题  

The transfer of biomolecules onto a membrane support 1)Preparing a Cell -free extract containing the biomolecule(s) of interest 2)Resolve the mixture by gel electrophoresis 3)Transfer the gel onto a membrane support 4)Incubate the membrane with a detection system that specifically binds to the molecule of interest

Southerns - Detection of DNA fragments on a blot Northerns - Detection of RNA fragments on a blot Westerns - Detection of proteins on a blot South-Westerns - Detection of DNA bound to proteins on a blot