Mapping, PCR DNA Sequencing

Software for:

Making Restriction Maps

Designing PCR primers

Assembling fragments, detecting SNPs, and managing raw sequence data

For:GCG,Mac,PC,UNIX,and on the Web

Restriction Mapping

-Making restriction maps was my first use of “Molecular Biology” software

-Making restriction maps is a routine lab activity that is necessary for any type of cloning project.

-High quality maps are important for publications and exchange of information between researchers or between labs.

Archiving Data

-Maps are a common way for labs to archive information about entire libraries of plasmid constructs.

-It is important that map data are stored in a reliable format so that the obsolescence of a particular computer program does not render archives unusable.

Mapping Software

-Programs vary greatly in sophistication and ease of use

-Simple drawing programs (vector graphics)

-The venerable DNA Strider

-GCG (not a strong point of the package)

-Comprehensive Mac/PC MolBio programs

-Dedicated plasmid drawing programs

-Can it be done on the Web?

-Making high quality graphical restriction maps is one area where Mac/PC programs are much better than GCG or the Web