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不能不知的细胞培养的全攻略
相关专题基础篇-无菌操作基本技术1. 实验进行前，无菌室及无菌操作台(laminar flow) 以紫外灯照射30-60 分钟灭菌，以70 %ethanol 擦
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Rapamycin-Induced Enhancement of Vaccine Efficacy in Mice
Th1 immunity protects against tuberculosis infection in mice and humans. The wid
2025-01-07 -
Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells
Embryonic stem cells (ESCs) are pluripotent cells derived from preimplantation e
2025-01-07 -
Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion
Ouabain, a toxic of vegetal origin used for centuries to treat heart failure, ha
2025-01-07 -
Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila
Kinetochores are essential for the proper positioning, movement and segregation
2025-01-07 -
Analysis of Common Mitochondrial DNA Mutations by Allele-Specific Oligonucleotide and Southern Blot
Mitochondrial disorders are clinically and genetically heterogeneous. There are
2025-01-07 -
Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells
Research of stem cells will enable us to understand the development and function
2025-01-07 -
应力纤维(stree fibers)
应力纤维又叫张力纤维，是真核细胞中广泛存在的一种较为稳定的束状纤维结构, 与骨骼肌中肌原纤维非常相像。应力纤维由大量平行排列的肌动蛋白组成,此外还含有其他在肌细
2025-01-07 -
Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line
Cell culture is an invaluable tool for investigators in numerous fields. It faci
2025-01-07 -
Conventional Micropipette-Based Patch Clamp Techniques
The patch clamp technique revolutionized the study of ion channels and is consid
2025-01-07 -
Scale-Up of Suspension and Anchorage-Dependent Animal Cells
In this chapter, scale-up is described in a laboratory context (10–20 L), but th
2025-01-07 -
On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis
This chapter reviews the theory and methodological developments of on-line conce
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Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens
The process of bringing a new pharmacologically active drug to market is laborio
2025-01-07 -
Measurement of Geminin Activity in Xenopus Egg Extracts
Geminin is an unstable protein that inhibits DNA replication by interfering with
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Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics: Constructing GFP-Tagged Motor Enz
The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequore
2025-01-07
