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Ultraviolet-Induced Apoptosis in Embryonic Stem Cells In Vitro
Embryonic stem (ES) cells, and the inner cell mass from which they are derived,
2025-01-06 -
Automation of Fluorescent Differential Display With Digital Readout
Since its invention in 1992, differential display (DD) has become the most commo
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Determination of Nuclear Localization Signal Sequences for Krppel-Like Factor 8
Transcription factor proteins function in the nucleus to regulate gene expressio
2025-01-06 -
FISH and Immunofluorescence Staining in Chlamydomonas
Here we describe how to use fluorescence in situ hybridization and immunofluores
2025-01-06 -
Preparation of low density, collagenase-digested splenocytes Protocol
Preparation of low density, collagenase-digested splenocytes Protocol 1. Dilute
2025-01-06 -
Isolation, Characterization, and Culture of Epithelial Stem Cells
It is well accepted that homeostasis of continuously renewing adult tissues, suc
2025-01-06 -
Tissue-Specific KO of ECM Proteins
The analysis of phenotypes caused by null and mutant alleles is a very powerful
2025-01-06 -
In Vitro Replication Assay with Mammalian Cell Extracts
Regulatory mechanisms for DNA replication are crucial to the control of the cell
2025-01-06 -
常用的蛋白水解酶抑制剂
当细胞被裂解，内容物释放时，蛋白水解酶和其他一些降解的酶也一同被释放，这时候需要在裂解的缓冲液中加入蛋白水解酶。PS: 细胞自己的蛋白水解酶也会降解细胞内的蛋白
2025-01-06 -
RNA 凝胶电泳注意事项
RNA 凝胶是用Northern 印迹的方法来分析RNA 。mRNA 大约只占总RNA 的5% ，在溴化乙锭染色的胶上看不到，因此mRNA 必须用标记探针来检测
2025-01-06 -
分子实验什么时候需要无菌操作？
当必须进行无菌操作时。无论什么时候在进行活的有机体实验或是为进行活体实验而准备培养基、缓冲液、培养容器等时，必须使用无菌操作技术，例如：· 准备转化用的大肠杆菌
2025-01-06 -
siRNA表达载体的构建
siRNA表达载体构建可以：（1）作为后基因组时代基因功能分析的有力工具；（2）应用于基因组学、细胞信号传导通路分析；（3）用于药物靶点筛选、疾病治疗。实验方法
2025-01-06 -
Markers of Adult Neural Stem Cells
I provide detailed protocols for conduction and troubleshooting the key steps in
2025-01-06 -
Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry
Isothermal titration calorimetry (ITC) is a useful technique to study RNA-protei
2025-01-06 -
Neutrophil Motility In Vivo Using Zebrafish
Zebrafish have emerged as a powerful model organism to study neutrophil chemotax
2025-01-06
