-
DNA甲基化实验操作步骤
DNA甲基化检测第一步：引物设计在GenBank上找到待检基因启动子区的原始序列，输入到“MethPrimer”软件中，输入启动子序列，选择“Pick prim
2025-02-11 2 -
Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Built-In Pillar Array
In vitro culture of mammalian cells is fundamental to various biological studies
2025-02-11 0 -
Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes
Studies using cultured cells allow one to dissect complex cellular mechanisms in
2025-02-11 0 -
六种甲基化检测方法
1、全基因组重亚硫酸盐甲基化测序（WGBS）全基因组重亚硫酸盐甲基化测序（WGBS）可以在全基因组范围内精确的检测所有单个胞嘧啶碱基（C碱基）的甲基化水平，是D
2025-02-11 1 -
Fluorescent Differential Display
Since their first introduction in 1992 (1 ,2 ), differential display (DD) and it
2025-02-11 0 -
研究新热点之细胞焦亡
细胞焦亡（Pyroptosis）又称细胞炎性坏死，是一种程序性细胞死亡，表现为细胞不断胀大直至细胞膜破裂，导致细胞内容物的释放进而激活强烈的炎症反应。细胞焦亡是
2025-02-11 0 -
Analysis of Janus Tyrosine Kinase Phosphorylation and Activation
Activation of Janus kinases (Jaks) occurs through autophosphorylation of key tyr
2025-02-11 0 -
细胞焦亡的研究方法与检测方式
首先是细胞焦亡的特点：细胞焦亡发生时，细胞会发生肿胀，在细胞破裂之前，细胞上形成凸出物(图1，早期细胞焦亡)，之后细胞膜上形成孔隙，使细胞膜失去完整性，释放内容
2025-02-11 1 -
EMS Screens: From Mutagenesis to Screening and Mapping
The success of Drosophila as a genetic model organism is based on the efficient
2025-02-11 0 -
Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells
The inability to measure mechanical forces within cells has been limiting our un
2025-02-11 0 -
Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos
The use of the confocal microscope to study living Xenopus eggs affords the oppo
2025-02-11 0 -
细胞焦亡在临床各领域的研究现状
首先是细胞焦亡与感染性疾病，也是我们在对细胞焦亡的研究中最简单也最广泛的。 在病原体感染时，适度的细胞焦亡可清除致病微生物，而过度的细胞焦亡在导致细胞死亡
2025-02-11 0 -
In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET
The emerging technique of bioluminescence resonance energy transfer (BRET) allow
2025-02-11 0 -
Quantitative Fluorescence In Situ Hybridization on Paraffin Embedded Tissue
Quantitative fluorescence in situ hybridization (Q-FISH) is a complex technique
2025-02-11 0 -
Fluorescence Immunohistochemistry in Combination with Differential Interference Contrast Microscopy
We have developed a technique, using a combination of immunofluorescent staining
2025-02-11 1
