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  • DNA的乙醇沉淀

    DNA的乙醇沉淀

    This procedure allows the concentration of DNA samples from dilute solution and

    2024-10-11
  • 人类基因组DNA提取及琼脂糖凝胶电泳分析

    人类基因组DNA提取及琼脂糖凝胶电泳分析

    实验目的:1. 熟悉分子生物学实验的操作特点。2.掌握人类基因组DNA提取的基本原理。3.熟悉人类基因组DNA提取的基本方法。4.熟悉琼脂糖凝胶电泳的原理和操作

    2024-10-11
  • 动物组织块DNA的提取

    动物组织块DNA的提取

    实验目的1. 学习并掌握动物组织总DNA的提取方法及其原理。2. 从肝脏组织中提取到一定量的纯净的DNA样品。实验原理DNA是一切生物细胞的重要组成成分,主要存

    2024-10-11
  • TRIzol法同时提取RNA、DNA、蛋白质

    TRIzol法同时提取RNA、DNA、蛋白质

    TRIzol试剂适用于从细胞和组织中快速分离RNA。TRIzol试剂有多组分分离作用,与其他方法如硫氰酸胍/酚法、酚/SDS法、盐酸胍法、硫氰酸胍法等相比,最大

    2024-10-11
  • 关于全血的保存-DNA提取-pcr个人经验

    关于全血的保存-DNA提取-pcr个人经验

    做了好多DNA提取保存,吃了很多亏给大家点建议吧:(有疑问可以回帖,有时间我会回答如果我知道)。  血液保存:  EDTA抗凝最好,肝素也可以不过经常会影响后续

    2024-10-11
  • 乙醇沉淀DNA实验操作方法

    乙醇沉淀DNA实验操作方法

    1. 加入 1/10 体积的乙酸钠(3 mol/L ,PH=5.2)于 DNA 溶液中充分混匀,使其 最终浓度为 0.3 mol/L;  2. 加入 2 倍体积

    2024-10-11
  • DNA抽提指南(TRIZOL)

    DNA抽提指南(TRIZOL)

    按照RNA 分离操作方案在完全移去水样层后,匀浆中的DNA 存在于中间层和苯酚层中也可以被分离出来。在沉淀和多次洗脱后,DNA 溶解在8 mM NaOH中。用

    2024-10-11
  • Chemical Sequencing of DNA

    Chemical Sequencing of DNA

    This is a rapid method for chemical DNA sequencing which is commonly used as lad

    2024-10-11
  • PREPARATION OF SEQUENCING GELS

    PREPARATION OF SEQUENCING GELS

    MATERIALS:2-glass plates1 sharks -tooth comb and spacersWhatman 3 mm paper30 or

    2024-10-11
  • DNA SEQUENCING REACTIONS

    DNA SEQUENCING REACTIONS

    DNA priming reactionx ul DNA 2 ul Reaction buffer, minus DTT 1 ul prim

    2024-10-11
  • NO "WEDGE" Sequencing Gels

    NO "WEDGE" Sequencing Gels

    I have been using an alternative to wedge gels that saves acrylamide, cuts gel d

    2024-10-11
  • Chemical Sequencing of DNA

    Chemical Sequencing of DNA

    This is a rapid method for chemical DNA sequencing which is commonly used as lad

    2024-10-11
  • DNA Sequencing Gels

    DNA Sequencing Gels

    Buffers and gel solutions Long Ranger: we started using this in early 1995. Grea

    2024-10-11
  • SEQUENCING GELS

    SEQUENCING GELS

    10X TBE, per liter Sequencing dye ------------------- ----------

    2024-10-11
  • Plasmid Sequencing

    Plasmid Sequencing

    This protocol gives M13 quality sequence when used with the Quick DNA Plasmid Pr

    2024-10-11