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DNA的乙醇沉淀
This procedure allows the concentration of DNA samples from dilute solution and
2024-10-11 -
人类基因组DNA提取及琼脂糖凝胶电泳分析
实验目的:1. 熟悉分子生物学实验的操作特点。2.掌握人类基因组DNA提取的基本原理。3.熟悉人类基因组DNA提取的基本方法。4.熟悉琼脂糖凝胶电泳的原理和操作
2024-10-11 -
动物组织块DNA的提取
实验目的1. 学习并掌握动物组织总DNA的提取方法及其原理。2. 从肝脏组织中提取到一定量的纯净的DNA样品。实验原理DNA是一切生物细胞的重要组成成分,主要存
2024-10-11 -
TRIzol法同时提取RNA、DNA、蛋白质
TRIzol试剂适用于从细胞和组织中快速分离RNA。TRIzol试剂有多组分分离作用,与其他方法如硫氰酸胍/酚法、酚/SDS法、盐酸胍法、硫氰酸胍法等相比,最大
2024-10-11 -
关于全血的保存-DNA提取-pcr个人经验
做了好多DNA提取保存,吃了很多亏给大家点建议吧:(有疑问可以回帖,有时间我会回答如果我知道)。 血液保存: EDTA抗凝最好,肝素也可以不过经常会影响后续
2024-10-11 -
乙醇沉淀DNA实验操作方法
1. 加入 1/10 体积的乙酸钠(3 mol/L ,PH=5.2)于 DNA 溶液中充分混匀,使其 最终浓度为 0.3 mol/L; 2. 加入 2 倍体积
2024-10-11 -
DNA抽提指南(TRIZOL)
按照RNA 分离操作方案在完全移去水样层后,匀浆中的DNA 存在于中间层和苯酚层中也可以被分离出来。在沉淀和多次洗脱后,DNA 溶解在8 mM NaOH中。用
2024-10-11 -
Chemical Sequencing of DNA
This is a rapid method for chemical DNA sequencing which is commonly used as lad
2024-10-11 -
PREPARATION OF SEQUENCING GELS
MATERIALS:2-glass plates1 sharks -tooth comb and spacersWhatman 3 mm paper30 or
2024-10-11 -
DNA SEQUENCING REACTIONS
DNA priming reactionx ul DNA 2 ul Reaction buffer, minus DTT 1 ul prim
2024-10-11 -
NO "WEDGE" Sequencing Gels
I have been using an alternative to wedge gels that saves acrylamide, cuts gel d
2024-10-11 -
Chemical Sequencing of DNA
This is a rapid method for chemical DNA sequencing which is commonly used as lad
2024-10-11 -
DNA Sequencing Gels
Buffers and gel solutions Long Ranger: we started using this in early 1995. Grea
2024-10-11 -
SEQUENCING GELS
10X TBE, per liter Sequencing dye ------------------- ----------
2024-10-11 -
Plasmid Sequencing
This protocol gives M13 quality sequence when used with the Quick DNA Plasmid Pr
2024-10-11