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外核膜(outer nuclear membrane)
外核膜面向细胞质基质, 常附有核糖体, 有些部位与内质网相连, 因此在形态和性质上与内质网相似。实际上, 外核膜可以看成是内质网膜的一个特化区。另外,细胞骨架成
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细胞培养基中的添加剂及其作用
相关专题 细胞培养基的配制 细胞培养相关用品 细胞培养专题· 培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞,很可能在DMEM或M199中同样很容易生
2025-02-11 3 -
人类人工染色体的构建策略
相关专题 摘要: 目前各种应用于基因治疗领域的载体均存在一定的局限性。越来越多的研究者认为,最理想的基因治疗载体应该在染色体 的结构基础上构建,使目的基因整合
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Apoptosis TUNEL Assay (frozen sections)
Protocol for Frozen Sections: Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix s
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奢侈基因(luxury gene)
即组织特异性(tissue-specific gene)表达的基因,。这类基因与各类细胞的特殊性有直接的关系, 是在各种组织中进行不同的选择性表达的基因。如表皮
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Membrane Protein Solubilization
A critical step in any in vitro analysis of membrane proteins is the solubilizat
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Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells
Hematopoietic stem cells (HSCs) present in small numbers in adult bone marrow (B
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A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Respo
Spermine oxidase (SMO), the most recently characterized polyamine metabolic enzy
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Matrigel invasion assays
Matrigel invasion assaysPosted on Saturday, October 18, 2003 DescriptionMatirgel
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Microscale Thermophoresis for the Assessment of Nuclear Protein-Binding Affinities
The rapid advance in our knowledge of cellular regulatory mechanisms, including
2025-02-11 1 -
Cryopreservation of Human Red Blood Cells
The red blood cell (RBC), or erythrocyte, is a flexible biconcave disc 8 �m in d
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A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays
A variety of techniques can be used to find protein partners, including immunopr
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Immobilization of Enzymes on Glyoxyl Agarose: Strategies for Enzyme Stabilization by Multipoint Atta
Immobilization of enzymes on pre-existing supports containing small aliphatic al
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Bovine Somatic Cell Nuclear Transfer
Somatic cell nuclear transfer (SCNT) is a technique by which the nucleus of a di
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Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells
The membrane organelles of an organized tissue, such as rat liver, that will alw
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