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细胞培养中的黑胶虫问题
1.我们实验室最近一次的情况: 我们前后从上海细胞中心买了三批细胞，细胞刚到的时候，观察均生长良好，密度适中，培养液清亮，也没有见小黑点，但是对细胞传代以后就陆
2025-02-25 1 -
Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins
Fusion constructs between G-protein-coupled receptors and G-protein α-subunits h
2025-02-25 1 -
Isolation, Purification, and Growth of Human Skeletal Muscle Cells
Skeletal muscle cells can be used in vitro for the study of myogenests, as well
2025-02-25 3 -
Live Imaging Mouse Embryonic Development: Seeing Is Believing and Revealing
The use of genetically encoded fluorescent proteins has revolutionized the field
2025-02-25 0 -
Promoter Analysis of TGF-Responsive Genes by Transient Transfection and Deletional/Mutational Analys
The tranforming growth factor β (TGF-β ) family of cytokines participates in reg
2025-02-25 0 -
Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for
Giant unilamellar vesicles (GUVs) constitute a cell-sized model membrane system
2025-02-25 0 -
Induction of Ca2+ Flux by Adhesion Molecules in Lymphocytes
Integrins participate in numerous complex biological processes that include cell
2025-02-25 0 -
Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxyg
Bone-derived stroma cells contain a rare subpopulation, which exhibits enhanced
2025-02-25 0 -
Determination of the Clinical Significance of an Unclassified Variant
After completion of Human Genome Project (HGP) in 2003, as well as the new techn
2025-02-25 0 -
A Live Cell Micro-imaging Technique to Examine Platelet Calcium Signaling Dynamics Under Blood Flow
The platelet is a specialized adhesive cell that plays a key role in thrombus fo
2025-02-25 0 -
DNA laddering assay for treated cells
Characteristics of this procedure: I found the procedure described by Gong et al
2025-02-25 1 -
Detecting Recruitment of DNA Damage Response Factors Through the eChIP Approach
DNA interstrand crosslinks (ICLs) are lesions that covalently link the two stran
2025-02-25 1 -
Transfection and Promoter Analysis in Embryonic Stem Cells
Embryonic stem (ES) cells are useful for the molecular analysis of developmental
2025-02-25 1 -
Methods to Analyze Transglutamination of Proteins Involved in Apoptosis
Enhanced expression of transglutaminases is a frequent, though not obligatory ph
2025-02-25 1 -
Scanning for Transcription Factor Binding by a Variant EMSA
Detection of in vitro protein–DNA interaction is one of many investigational ana
2025-02-25 1
