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Retrovirus Production
Material:Packaging Cells, e.g. Phoenix cells (an adenovirus Ad5-transformed huma
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细胞凋亡检测:流式细胞仪检测技术
细胞调亡的流式细胞仪检测技术 一、固定细胞的染色方法 1 ) PI 单染色法 方法一1. 收集细胞( 1 ~ 5 ) 106 个, 500 ~ 1000r/mi
2025-02-28 2 -
Hemacytometer Workbook
This workbook was developed for use with Module 2 of the InVitro Insights Cell C
2025-02-28 0 -
细胞电泳
在一定PH值下细胞表面带有净的正或负电荷,能在外加电场的作用下发生泳动,这种现象称为细胞电泳(cell electrophoresis)。引起细胞电泳的电位值称
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蛋白激酶A (protein kinase A,PKA)
又称依赖于cAMP的蛋白激酶A (cyclic-AMP dependent protein kinase A),是一种结构最简单、生化特性最清楚的蛋白激酶。PK
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Lyophilization of Nonviral Gene Delivery Systems
This chapter presents a qualitative description of the freeze-drying process as
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Definitions and Criteria for Stem Cells
The working definition of a stem cell includes self-renewal and the ability to d
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Isolating primary B cells from human blood
1. start with freshly drawn human blood not older than 8 hours, treated with an
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线粒体膜势能检测细胞凋亡
线粒体在细胞凋亡的过程中起着枢纽作用,多种细胞凋亡刺激因子均可诱导不同的细胞发生凋亡,而线粒体跨膜电位的下降,被认为是细胞凋亡级联反应过程中最早发生的事件,它
2025-02-28 2 -
大肠杆菌杂交
相关专题 一、实验原理Lederberg和Tatum(1946)选用典型的大肠杆菌为材料,筛选营养缺陷型。利用双重和三重缺陷型的菌株,在简单的合成培养基上混合
2025-02-28 3 -
嘌呤霉素(puromycin)
嘌呤霉素是一种蛋白质合成抑制剂,它具有与tRNA分子末端类似的结构, 能够同氨基酸结合,代替氨酰化的tRNA同核糖体的A位点结合,并掺入到生长的肽链中。虽然嘌呤
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小鼠的R1胚胎干细胞系培养实验步骤
相关专题 以下是根据NIH老年病研究所 提供的英文的R1胚胎干细胞培养protocol整理而成。根据经验作部分修改。1、一般培养:保持胚胎干细胞 处于未分化状
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兔子白细胞介素10(IL-10)酶联免疫分析
兔子白细胞介素10 (IL-10) 酶联免疫 分析试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定兔子血清,血浆,细胞上清及相关液
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Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
Human hematopoietic stem cells (HSCs) and progenitors can be isolated by enrichi
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Induction of Tumor Cell Apoptosis and Chemosensitization by Antisense Strategies
Radiation and traditional chemotherapy kill both normal and tumor cells owing to
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