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Isolation, Characterization, and Culture of Epithelial Stem Cells
It is well accepted that homeostasis of continuously renewing adult tissues, suc
2025-01-06 -
Tissue-Specific KO of ECM Proteins
The analysis of phenotypes caused by null and mutant alleles is a very powerful
2025-01-06 -
In Vitro Replication Assay with Mammalian Cell Extracts
Regulatory mechanisms for DNA replication are crucial to the control of the cell
2025-01-06 -
常用的蛋白水解酶抑制剂
当细胞被裂解，内容物释放时，蛋白水解酶和其他一些降解的酶也一同被释放，这时候需要在裂解的缓冲液中加入蛋白水解酶。PS: 细胞自己的蛋白水解酶也会降解细胞内的蛋白
2025-01-06 -
RNA 凝胶电泳注意事项
RNA 凝胶是用Northern 印迹的方法来分析RNA 。mRNA 大约只占总RNA 的5% ，在溴化乙锭染色的胶上看不到，因此mRNA 必须用标记探针来检测
2025-01-06 -
分子实验什么时候需要无菌操作？
当必须进行无菌操作时。无论什么时候在进行活的有机体实验或是为进行活体实验而准备培养基、缓冲液、培养容器等时，必须使用无菌操作技术，例如：· 准备转化用的大肠杆菌
2025-01-06 -
siRNA表达载体的构建
siRNA表达载体构建可以：（1）作为后基因组时代基因功能分析的有力工具；（2）应用于基因组学、细胞信号传导通路分析；（3）用于药物靶点筛选、疾病治疗。实验方法
2025-01-06 -
Markers of Adult Neural Stem Cells
I provide detailed protocols for conduction and troubleshooting the key steps in
2025-01-06 -
Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry
Isothermal titration calorimetry (ITC) is a useful technique to study RNA-protei
2025-01-06 -
Neutrophil Motility In Vivo Using Zebrafish
Zebrafish have emerged as a powerful model organism to study neutrophil chemotax
2025-01-06 -
OnLine Ozonolysis Methods for the Determination of Double Bond Position in Unsaturated Lipids
Modern lipidomics relies heavily on mass spectrometry for the structural charact
2025-01-06 -
Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells
The unlimited differentiation and proliferation capacity of embryonic stem cells
2025-01-06 -
Expression Analysis of Murine Genes Using In Situ Hybridization With Radioactive and Nonradioactivel
The term in situ hybridization (ISH) refers to all methods allowing the detectio
2025-01-06 -
细胞融合技术
相关专题 细胞融合(Cell fusion)或细胞杂交(cell hybridization)是指真核细胞通过介导和培养，两个或多个细胞合并成一个双核或多核细
2025-01-06 -
Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells
Our laboratory has been engaged in studies of lipid hydrolases since the early 1
2025-01-06
