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Production and Characterization of Recombinant Human and Murine TNF
Here we describe the methods for the expression of human and murine soluble tumo
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The Use of Phage Display Peptide Libraries for Basic and Translational Research
Phage display is a molecular technique, whereby genes are displayed in a functio
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Basic Immunocytochemistry for Light Microscopy
Immunocytochemistry may be defined as the identification of a cell- or tissue-bo
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Identification of DNA-Binding of Tumor Suppressor Genes by Chromatin Immunoprecipitation
The processes of DNA replication and transcription are intimately affected by th
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Genetic Polymorphisms in the Transforming Growth Factor-β Signaling Pathways and Breast Cancer Risk
The transforming growth factor (TGF)-β signaling pathway plays a critical role i
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Quantitative Immunohistochemistry of Estrogen and Progesterone Receptor Positivity in Ovarian Tumors
Several assays exist to determine receptor status in ovarian cancers, like radio
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Detection of Circulating Tumor Cells by Photoacoustic Flowmetry
Detection of circulating tumor cells (CTCs) in human blood and lymph systems has
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Development of Bacterial Vectors for Tumor-Targeted Gene Therapy
Gene therapy holds great promise for the treatment of cancer. The success of the
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Analysis of EMT by Flow Cytometry and Immunohistochemistry
Ovarian cancer stem cells (OCSCs) are in a transitional phase between epithelial
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Prediction of the Biological Effect of Polymorphisms Within MicroRNA Binding Sites
MicroRNAs (miRNAs) are negative gene regulators acting at the 3′UTR level, modul
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Comparative Genomic Hybridization
Comparative genomic hybridization (CGH ) allows a genome-wide survey of the rela
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Breast Cancer Proteomics Using Two-Dimensional Electrophoresis: Studying the Breast Cancer Proteome
Proteomics has emerged as a powerful approach for studying disease-associated ch
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PCR Microsatellite Analysis of LOH in Ovarian Tumors
The “two-hit” theory of tumor suppressor gene (TSG) inactivation predicts that l
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The EGFR Pathway as an Example for Genotype: Phenotype Correlation in Tumor Genes
Tumor-specific and germ-line variations of DNA significantly contribute to tumor
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Transfection Assays for Transformation of Model Colonic Cell Lines
The recognition of cancer as a genetic disease has changed the approach investig
