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实验室一些常用知识介绍
相关专题 一.玻璃仪器的洗涤及一些常用的洗涤剂1. 玻璃仪器的洗涤(1)新购买的玻璃仪器, 首先用自来水洗去表面灰垢, 然后用洗衣粉刷洗, 自来水冲净后, 浸
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Column Purification of Demethylated Sphingomyelin
Packing column: 1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of ch
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小鼠雌激素(E)酶联免疫分析(ELISA)
小鼠雌激素(E) 酶联免疫 分析( ELISA )试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定小鼠血清,血浆及相关液体样本中 雌激素(E) 含量
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球蛋白(glubulin)
能被 50%饱和度硫酸铵溶液沉淀的单纯蛋白质。不溶于纯水,易溶于稀盐水,加热即沉淀或凝固。存在于所有动植物细胞和体液中。最熟知的球蛋白是人血清球蛋白,可经电泳
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小鼠β-肌动蛋白(β-actin)酶联免疫分析(ELISA)
小鼠β- 肌动蛋白 ( β -actin) 酶联免疫 分析( ELISA )试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定小鼠血
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镁的代谢
第二节 镁的代谢镁(magnesium)的代谢及功能与钙、磷有密切关系。人体含镁量约20-28g,一半以上存在于骨中,其余在细胞内,是细胞内重要阳离子之一。细胞
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生物氧化酶类
体内催化氧化反应的酶有许多种,按照其催化氧化反应方式不同可分为三大类。(一)脱氢氧化酶类这一类中依据其反应受氢体或氧化产物不同,又可以分为三种。1.氧化酶类(o
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Adipose Tissue-Specific PPAR Gene Targeting
The nuclear receptor peroxisome proliferator activated receptor gamma (PPARγ) is
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肝脏的化学组成特点
正常人肝脏重约1-1.5kg,其中水份占70%。除水外,蛋白质含量居首位。已知肝脏内的酶有数百种以上,而且有些酶是其它组织中所没有或含量极少的。例如合成酮体和尿
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Superoxide Production in the Islet of Langerhans Detected by the MCLA Chemiluminescence Method
It is well known that the superoxide anion radical (O-2 ) is produced in the bod
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High-Density Labeling of DNA for Single Molecule Sequencing
Two unusual enzymatic activities are required for the realization of a single mo
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Crystallization of Cyclic Nucleotide Phosphodiesterases
Selective inhibitors of cyclic nucleotide phosphodiesterases (PDEs) have been wi
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[3H] Choline Labeling and TNF Treatment of HL-60 Cells
1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.
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Dereplication and Partial Identification of Natural Products
Since the “Golden Age of Antibiotics” in the 1950s, natural products chemists ha
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FOUCHET METHOD stain for bile
1. Sections to distilled water.2. Stain in Fouchets reagent 5 minutes.3. Rinse i
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