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Protocols for Use of Homologous Recombination Gene Targeting to Produce MicroRNA Mutants in Drosophi
MicroRNAs (miRNAs) are noncoding RNA molecules that have come to attract conside
2025-01-07 -
Genetic Modification of Murine Hematopoietic Stem Cells by Retroviruses
Among the currently available methods for gene transfer, recombinant murine retr
2025-01-07 -
Quantitation of Cocaine, Benzoylecgonine, Ecgonine Methyl Ester, and Cocaethylene in Urine and Blood
Cocaine, a stimulant, is a commonly abused drug. Cocaine and its metabolites are
2025-01-07 -
Assaying Cell-Cell Adhesion
A major feature in Dictyostelium development is the transition from the unicellu
2025-01-07 -
多能干细胞（pluripotent stem cell）
多能干细胞具有分化出多种细胞组织的潜能，但失去了发育成完整个体的能力，发育潜能受到一定的限制。骨髓多能造血干细胞是典型的例子，它可分化出至少十二种血细胞，但不能
2025-01-07 -
核纤层蛋白(nuclear lamina protein)
脊椎动物细胞中有三种类型的核纤层蛋白(A,B,C), 核纤层蛋白A和C是由同一个转录单位编码的, 只不过是通过可变剪接形成不同的mRNA。它们在肽链上的差别是:
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Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry
Chemical modification reactions play an important role in various protocols for
2025-01-07 -
Cell death detection in Xenopus embryos by ELISA
Sample preparationWash embryos 1 x in 25% MMRRemove excess bufferAdd 10 volumes
2025-01-07 -
Examination of the Axial Skeleton of Fetal Rodents
The axial skeleton represents one product of the metameric segregation of the me
2025-01-07 -
Tissue Culture Methods
TYPES OF CELLS GROWN IN CULTURETissue culture is often a generic term that refer
2025-01-07 -
MAP Kinase Regulation of the Mitotic Spindle Checkpoint
Maintaining the integrity of the cell cycle is critical for ensuring that cells
2025-01-07 -
Derivation and Manipulation of Murine Embryonic Stem Cells
Pluripotent embryonic stem (ES) cell lines were first isolated over 25 years ago
2025-01-07 -
Isolation and Characterization of Hepatic Stem Cells, or Oval Cells, from Rat Livers
The pace of research on the potential therapeutic uses of liver stem cells or “o
2025-01-07 -
Biochemical Analysis of Arginine Methylation in Transcription
Protein arginine methylation has emerged as an important mechanism for regulatin
2025-01-07 -
Using Quantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Even
The mechanisms that mediate how migratory eukaryotic cells amplify a shallow, ex
2025-01-07
