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Competitive and Differential RT-PCR (CD-RT-PCR) for Measurement of Normalized Gene Expression Using
Quantitative mRNA characterization by reverse transcription (RT) of RNA and subs
2025-01-07 -
Overexpression or Downregulation of mTOR in Mammalian Cells
This chapter presents an overview of the methods that have been used to overexpr
2025-01-07 -
Control of the Interferon Response in RNAi Experiments
The RNA interference (RNAi) and interferons have been an uneasy marriage. Ever s
2025-01-07 -
Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi
Genome sequences of many filamentous fungi are now available and additional geno
2025-01-07 -
Screening for Protein-Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells
The two-hybrid system (THS) (1 ) is a molecular genetic screen that detects prot
2025-01-07 -
Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity
Cellular membranes, which contain abundant phospholipids, such as phosphatidylch
2025-01-07 -
A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae
Critical cellular processes such as DNA replication, DNA damage repair, and tran
2025-01-07 -
Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. cri
The glucosylation of 5-position of anthocyanin molecules is one of most importan
2025-01-07 -
协助扩散
物质通过质膜进出细胞的方式之一。物质必须在载体蛋白的协助下，才能从高浓度一侧通过质膜向低浓度一侧扩散，但此种方式不消耗代谢能。协助扩散和自由扩散都是物质顺浓度梯
2025-01-07 -
Methods to Study the Ras2 Protein Activation State and the Subcellular Localization of Ras-GTP in Sa
Ras proteins were highly conserved during evolution. They function as a point of
2025-01-07 -
Joining RNA Molecules with T4 DNA Ligase
Researchers interested in studying RNA structure and function or RNA-protein int
2025-01-07 -
Semipermeabilized Cells to Study Procollagen Assembly
This chapter will describe the preparation and use of a semipermeabilized (SP) c
2025-01-07 -
Approaches and Species in the History of Vertebrate Embryology
Recent debates about model organisms echo far into the past; taking a longer vie
2025-01-07 -
Novel Strategies for the Identification of Clock Genes Neurospora With Insertional Mutagenesis
As the molecular mechanism of circadian clocks has reached high complexity, the
2025-01-07 -
着丝粒(centromere)
染色体中连接两个染色单体, 并将染色单体分为两臂: 短臂(p)和长臂(q)的部位。由于此部位的染色质较细、内缢, 又叫主缢痕(primary constrict
2025-01-07
