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In Vitro Matrigel Angiogenesis Assays
A variety of in vivo and in vitro methods have been used to study angiogenesis,
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Locked Nucleic Acid Aptamers
The aptamer technology has been introduced in the early 1990s. With this techniq
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核仁组织区（nucleolus organiting region，NOR）
参与形成核仁时的染色质区，核仁从核仁组织区部位产生，同时与该区紧密相连。具有核仁组织区的染色体称核仁染色体。核仁组织区定位在核仁染色体次缢痕部位。对人来说，在1
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原核融合(pronuclei fussion)
精细胞的细胞核进入卵细胞质后，包装紧密的染色质开始解旋。精子的细胞核膜解体形成小的囊泡，并立即与精子细胞核脱离，形式没有核被膜的精子染色质，但很快形成新的核被膜
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分裂间期
真核细胞在细胞周期中，由上次细胞分裂结束到下次细胞分裂开始的持续时间。间期细胞进行旺盛的生物合成和生长，是细胞进入有丝分裂期的重要准备阶段。间期可划分为： G1
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细胞遗传学――原位杂交(ISH)
In Situ Hybridization・ In Situ Hybridization (jsmith1@po-box.mcgill.ca )
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Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis Assay
Successful therapeutic angiogenesis requires an understanding of how the milieu
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激光显微细胞分离技术
在基础医学研究中涉及越来越多的如某一疾病状态组织中，多种基因或遗传变化，区别发展中的组织细胞群以及疾病状态组织细胞，将有助于了解疾病发生的分子机制。因此，在下一
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Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damage
Mammalian cells remove carcinogenic damage caused to DNA by ultraviolet (UV) lig
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Keratinocyte Transient Transfections
The goal of this chapter is to give the reader a concise and easy-to-follow guid
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Quantitation of Sirolimus Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS)
A multiple reaction monitoring positive ion HPLC method with tandem mass spectro
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Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry
Direct MALDI-TOF mass spectrometric peptide profiling is increasingly used to an
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细胞生物基本方法：常规组织培养法
常规组织培养法 1 ）初代消化培养法 1. 准备：取各种已消毒的培养用品置于净化台面，紫外线消毒 20 分钟。开始工作前先洗手、 75% 酒精擦拭手至
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Well-Differentiated Human Airway Epithelial Cell Cultures
The airway epithelium occupies a critical environmental interface, protecting th
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Deep Sequencing of Small Chromatin-Associated RNA: Isolation and Library Preparation
Chromatin-associated RNA (caRNA) is a newly identified class of RNA molecules st
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