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Reproductive and Developmental Manipulation of the Marsupial, the Tammar Wallaby Macropus eugenii
The developing marsupial is an ideal animal for use in biomedical research. Mars
2025-02-08 1 -
肝纤维化动物造模步骤上篇
一、CCl4诱导大鼠肝纤维化模型实验步骤CCl4诱导大鼠肝纤维化模型的造模机制:CCl4为一种选择性肝毒性药物,其进入机体后在肝内活化成自由基如三氯甲基自由基(
2025-02-08 1 -
Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry
Protein phosphorylation is central to most signaling events in eukaryotic cells.
2025-02-08 1 -
Analytical Methods for Cancer Stem Cells
The primary characteristics of adult stem cells are maintaining prolonged quiesc
2025-02-08 1 -
Assessing BloodBrain Barrier Function Using In Vitro Assays
The impermeability of the blood–brain barrier (BBB) is due to a number of proper
2025-02-08 1 -
Separation of Synthetic (Co)Polymers by Capillary Electrophoresis Techniques
Capillary electrophoresis (CE) is a very efficient tool for separating and chara
2025-02-08 1 -
Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR)
Saturation mutagenesis is one approach for determining the contributions of indi
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合成代谢(anabolism)
亦称生物合成。指把小的前体物质(如α-酮酸等),或基本结构单位(如氨基酸、单糖、脂肪酸等)分子转化成大分子细胞组分(如蛋白质、核酸等)的过程,故又称同化作用。
2025-02-08 1 -
Cell Disruption and Subcellular Fractionation
Nitrogen Bomb 1) Harvest cells and wash 2 times with PBS. 2) Resuspend final pel
2025-02-08 1 -
Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye
The Alamar Blue assay is based on enzymatic reduction of indicator dye by viable
2025-02-08 1 -
Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis
MicroRNAs (miRNAs) are noncoding RNAs whose hallmarks are the very short sequenc
2025-02-08 1 -
Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions
Human cord blood (CB), collected from the postpartum placenta and umbilical cord
2025-02-08 2 -
肝纤维化动物造模步骤下篇
一、胆管结扎诱导肝纤维化动物模型1、实验准备雄性C57,BALB/c小鼠(10周龄左右)或雄性SD大鼠(200g左右)。器械:扩创钩,镊子,尖头剪刀,持针钳,圆
2025-02-08 1 -
羊水细胞染色体标本的制备
一、原理 人的羊水细胞能长成单层并可连续进行传代培养,但它们的一些特征与一般成纤维细胞不同。在羊水中存在着各种不同类型的胎儿细胞,依据其外形和生长特征可
2025-02-08 6 -
Mimicry of Protein Function with Cell-Penetrating Peptides
Proteins are essential components of cellular processes inside cells, and their
2025-02-08 2