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  • Reproductive and Developmental Manipulation of the Marsupial, the Tammar Wallaby Macropus eugenii

    Reproductive and Developmental Manipulation of the Marsupial, the Tammar Wallaby Macropus eugenii

    The developing marsupial is an ideal animal for use in biomedical research. Mars

    2025-02-08 1
  • 肝纤维化动物造模步骤上篇

    肝纤维化动物造模步骤上篇

    一、CCl4诱导大鼠肝纤维化模型实验步骤CCl4诱导大鼠肝纤维化模型的造模机制:CCl4为一种选择性肝毒性药物,其进入机体后在肝内活化成自由基如三氯甲基自由基(

    2025-02-08 1
  • Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry

    Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry

    Protein phosphorylation is central to most signaling events in eukaryotic cells.

    2025-02-08 1
  • Analytical Methods for Cancer Stem Cells

    Analytical Methods for Cancer Stem Cells

    The primary characteristics of adult stem cells are maintaining prolonged quiesc

    2025-02-08 1
  • Assessing BloodBrain Barrier Function Using In Vitro Assays

    Assessing BloodBrain Barrier Function Using In Vitro Assays

    The impermeability of the blood–brain barrier (BBB) is due to a number of proper

    2025-02-08 1
  • Separation of Synthetic (Co)Polymers by Capillary Electrophoresis Techniques

    Separation of Synthetic (Co)Polymers by Capillary Electrophoresis Techniques

    Capillary electrophoresis (CE) is a very efficient tool for separating and chara

    2025-02-08 1
  • Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR)

    Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR)

    Saturation mutagenesis is one approach for determining the contributions of indi

    2025-02-08 2
  • 合成代谢(anabolism)

    合成代谢(anabolism)

    亦称生物合成。指把小的前体物质(如α-酮酸等),或基本结构单位(如氨基酸、单糖、脂肪酸等)分子转化成大分子细胞组分(如蛋白质、核酸等)的过程,故又称同化作用。

    2025-02-08 1
  • Cell Disruption and Subcellular Fractionation

    Cell Disruption and Subcellular Fractionation

    Nitrogen Bomb 1) Harvest cells and wash 2 times with PBS. 2) Resuspend final pel

    2025-02-08 1
  • Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye

    Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye

    The Alamar Blue assay is based on enzymatic reduction of indicator dye by viable

    2025-02-08 1
  • Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis

    Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis

    MicroRNAs (miRNAs) are noncoding RNAs whose hallmarks are the very short sequenc

    2025-02-08 1
  • Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions

    Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions

    Human cord blood (CB), collected from the postpartum placenta and umbilical cord

    2025-02-08 2
  • 肝纤维化动物造模步骤下篇

    肝纤维化动物造模步骤下篇

    一、胆管结扎诱导肝纤维化动物模型1、实验准备雄性C57,BALB/c小鼠(10周龄左右)或雄性SD大鼠(200g左右)。器械:扩创钩,镊子,尖头剪刀,持针钳,圆

    2025-02-08 1
  • 羊水细胞染色体标本的制备

    羊水细胞染色体标本的制备

    一、原理 人的羊水细胞能长成单层并可连续进行传代培养,但它们的一些特征与一般成纤维细胞不同。在羊水中存在着各种不同类型的胎儿细胞,依据其外形和生长特征可

    2025-02-08 6
  • Mimicry of Protein Function with Cell-Penetrating Peptides

    Mimicry of Protein Function with Cell-Penetrating Peptides

    Proteins are essential components of cellular processes inside cells, and their

    2025-02-08 2