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Recording Dendritic Ion Channel Properties and Function from Cortical Neurons
Dendrites emerging from the cell bodies of neurons receive the majority of synap
2025-02-08 1 -
Biological Membranes as Protein Aggregation Matrices and Targets of Amyloid Toxicity
Aberrantly folded proteins and peptides are hallmarks of amyloid diseases. A dee
2025-02-08 1 -
Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Developm
Delayed implantation, a reversible arrest in embryo development while the embryo
2025-02-08 1 -
Live-Cell Imaging of Microtubule Dynamics in Hyphae of Neurospora crassa
Due to the large number of microtubules in the wild-type strain, total internal
2025-02-08 1 -
egakaryocyte and Platelet Production from Human Cord Blood Stem Cells
The cloning of thrombopoietin together with advances in the culture of hematopoi
2025-02-08 1 -
Assessment of Oxidative Stress in Sperm and Semen
The chemiluminescence method is the most commonly employed technique as a direct
2025-02-08 1 -
Molecular Profiling of Mitochondrial Dysfunction in Caenorhabditis elegans
Cellular effects of primary mitochondrial dysfunction, as well as potential mito
2025-02-08 1 -
脑缺血模型造模步骤—下
二血管阻塞慢性脑缺血模型是结扎大鼠双侧颈总动脉造成脑血流的下降并能较好模拟慢性缺血所致的病理改变,操作简单、重复性好、死亡率低,被广泛用于慢性脑缺血的研究。该模
2025-02-08 1 -
脑缺血动物造模实操注意事项
一、大脑中动脉栓塞(MCAO)模型造模操作注意事项1、分离时要层次清楚:正中切开SD大鼠颈部皮肤,分离皮下结缔组织时会发现该组织与颈部两侧鼓泡腺体(很多人误认为
2025-02-08 1 -
活性氧(ROS)检测介绍
氧是生命运动过程中不可缺少的一种气体,人们一旦处于缺氧或者供氧不足的环境中,就会感到憋气的痛苦甚至死亡。所以,自从发现氧以来氧一直被人们认为是一种对人体百益而无
2025-02-08 1 -
活性氧检测荧光法实验步骤
一、荧光法检测活性氧实验原理活性氧检测试剂盒(Reactive Oxygen Species Assay Kit,也称ROS Assay Kit)是一种利用荧光
2025-02-08 1 -
活性氧检测化学发光法实验步骤
近年来,随着光电检测技术的不断进步,化学发光分析法已在医学、环境以及工业分析等许多领域里得到广泛的应用。而活性氧也在人体内的作用受到人们极大的关注,报道了大量有
2025-02-08 1 -
什么是支原体检测?
支原体(mycoplasma)是一类没有细胞壁、高度多形性、能通过滤菌器、可用人工培养基培养增殖的最小原核细胞型微生物,大小为0.1~0.3微米。由于能形成丝状
2025-02-08 1 -
支原体检测方法大全
支原体在细胞进行培养、传代时极易污染。目前我们常用的支原体检测方法有PCR法、DNA染色法、化学发光法、支原体培养法,我们来一个一个看:1、PCR检测支原体这种
2025-02-08 2 -
PCR检测支原体操作步骤
支原体因为个头小,能穿过0.22μm滤器,并且在实验室内会潜在的扩散。支原体污染细胞后,培养液不一定会发生混浊。多数情况下细胞病理变化轻微或不显著,细微变化也可
2025-02-08 2