DNA FRAGMENT PURIFICATION W/ GLASS WOOL

Glass wool method:

Run TAE agarose gel and cut the appropriate band out with a clean razor blade.

Poke a small hole with hot needle on the bottom of an eppendorf tube, and jam the hole with a little of siliconized glass wool.

Put the gel in the tube and put this tube onto another tube and spin with 5-7 Krpm for 5-10 min. Add 1/2 vol. of phenol and 1/10 vol. of 3 M NaAc of the gel volume to the agarose tube and spin for another 5 min.

Discard the agarose tube. Add 1/2 vol. of chloroform to the liquid and spin at 14 k for 5'. Take the top layer and precipitate with 2-2.5 vol. of ethanol for 30 '. Spin and wash with 70% ethanol.