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Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
Low (“Low”)Imidazole Buffer 0.5L100mM Imidizole 3.4g5% glycerol 25ml 100% glycer
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DEAE column: FPLC
Contributor: Suprya JayadevDate: September 21,1993Resin preparation:1)Measure ou
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Protocols for protein extraction from whole tissues for isoelectric focusing
SDS extraction followed by acetone precipitation – simple extraction protocol th
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ULTIMATE FREEZE-THAW LYSIS FOR MAMMALIAN CELLS
This extraction procedure was developed by the Simmlaboratory and described in R
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Purification of GST fusion proteins in E.coli GST融合蛋白纯化，方法一
Making GST fusion proteins:(07/19/03)ver.1Grow up 5ml LB with Amp o/n.Add to 45m
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Preparation of cell lysates from E. coli by enzymatic lysis
Preparation of cell lysates from E.coli by enzymatic lysisMaterialsChemicalslyso
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Taq Polymerase Purification
This is a protocol from Engelke et.al.(1990)which was modified by Dr.Baron.The e
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Cell Lysates For Western Blotting
Reagents / SolutionsLysis Buffer:10ml 10% Sodium dodecyl sulphate (SDS)10ml Glyc
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Affinity Column Preparation 亲和层析柱制备
1.Prepare 1 ml of packed beads by washing them 4 times in 10 ml of d-H2O in a 15
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DEAE Column
Contributor: Suprya JayadevDate: April 11,19961)Weigh out the appropriate amount
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Preparation of Brain Membrane Fractions
Preparation of Brain Membrane Fractions for Western Blot AnalysisAll the procedu
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凝胶柱的清洗问题
问：最近在回收凝胶柱时（用的是镍柱），经过多次的洗脱液继续平衡清洗，发现还有一些很难去除的杂蛋白，不知应该用什么方法把这些难除的杂蛋白去除？答：一般用6M盐酸胍
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凝胶过滤填料的分离范围表
SephadexG-10葡聚糖凝胶 G-10 分离范围<700 适用于脱盐、肽与其它小分子的分离Sephadex G-15 葡聚糖凝胶 G-15 分离范围
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Chromatin Immunoprecipitation（ChIP）实验指南及技术总结
ChIP是一项比较流行的研究转录因子（ transcription factor, TF）与启动子（promoter）相互结合的实验技术。由于ChIP采用甲醛固
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天然蛋白的免疫共沉淀方法详解
本方法免疫沉淀得到的天然蛋白可用于 western 免疫印迹或是激酶活性分析所需溶液和试剂注意：所有溶液用反渗透去离子水（Reverse Osmosis Dei
