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qPCR原理和方法都在这里【新手入门】
各位小伙伴们下午好~疫情当前,作为新型冠状病毒的检测手段之一,想必大家对qPCR检测有一定的好奇,小编偷偷拿到了咱们技术老师的课件给大家来一波分享,往下看哟~P
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SSR标记介绍
什么是SSR?短串联重复序列(Short tandem repeat, STR)=微卫星DNA( microsatellite DNA) = 简单重复序列(si
SSR -
PCR新手必看!细节制胜
嘿,PCR新手们!刚开始做PCR实验可能会有点手忙脚乱,但别担心,我来给你们分享一些小贴士,帮你们顺利上手。实验设计 在开始实验之前,一定要仔细设计你的实验方案
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采用 qRT-PCR 检测细胞炎性因子
一、原理qPCR 技术通过引物特异性扩增目标 DNA 序列,并通过荧光探针或 SYBR Green I 荧光染料法检测 PCR 产物的数量,来定量分析样品中目标
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Amino acid composition
There has been a recent revival of interest in the use of AA composition for the
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聚丙烯酰胺凝胶技术
型号排阻的下限 (分子 量)分级分离范围 (分子量)膨胀后的床体积(ml/g干凝胶)膨胀所需最少时间(室温,h)bio-gel-p-21,600200~2,00
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Two Dimensional Gel Electrophoretic Analysis for the Human Plasma
Overview This protocol is a detail description of the laboratory procedure in
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SDS-polyacrylamide gel electrophoresis
SOLUTIONS30% acrylamide mix29% (w/v) acrylamide1% (w/v) N,N''- methylene
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RNA gel electrophoresis
Materials DEPC H2 O DEPC 0.1% (v/v) q.s. de-ioinized H2O 37ºC x1 hr, or r.t. ove
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Silver Staining SDS PAGE Gels
We currently use the SilverQuest Silver stain kit from Invitrogen. This method i
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Protein Gel Preparation and Staining
This is a protocol using the glycine system described by Laemmli, U.K. (1970) Na
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Agarose Gel Electrophoresis of DNA
Agarose Gel Electrophoresis of DNA1) Dissolve 1 g of agarose in 100 ml of 1X TAE
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制备凝胶板的方法
等电聚焦电泳与琼脂糖电泳过程中凝胶板的制备方法和简单操作等电聚焦电泳 1.制备凝胶板 1)30%凝胶母液的制备 丙烯酰胺30克,N、N双甲叉丙烯酰胺0.8克,加
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Native-PAGE常见问题
1. 非变性聚丙烯酰胺 凝胶电泳 时预电泳是怎么回事的?预电泳时要加6DNA上样缓冲液吗?电泳1-2小时再加结合反应产物吗?预电泳是除去凝胶中没有聚合的单体和双
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NuPAGE Gels
NuPAGE GelsA gel electrophoresis system used for SDS-PAGE protein analysis. The