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RT-PCR经验浅谈
做RNA病毒基因的RT-PCR成败的关键首先在于RNA模板的制备。本人三年前做过一个正链RNA病毒全基因组分段扩增，设计方案是将全基因组分成7个片段，0.6kb
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What is the highest temperature that reverse trascriptases can be us
Question What is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSC
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如何建立一个PCR实验室？
为了对以个特定序列进行PCR做重复检测,需要三个不同的区域,每一个区域的具体技术操作和试剂在下面详细列出.1、样品准备区 这个区域专门用作样品的准备，在制备和操
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PCR常见问题总汇(一）
PCR产物的电泳检测时间 一般为48h以内，有些最好于当日电泳检测，大于48h后带型不规则甚致消失。 假阴性，不出现扩增条带 PCR反应的关键环节有①模
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Microdeletion screening
Microdeletion screening One application of multiplex PCR is microdeletion screen
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Disruption by Fusion PCR
Disruption by Fusion PCR David Amberg and Ellen Beasley1) In separate PCR reacti
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CORE SAMPLE PCR: A method to re-PCR unique bands from products of mixed
INTRODUCTIONThe products of a PCR reaction - especially when this is done on euk
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Single Primer (Semi-Random) PCR
Single Primer ("Semi-Random") PCRJuly 26, 2000 ECKDescriptionSingle pr
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PCR常见问题总汇(三）
PCR反应体系与反应条件 标准的PCR反应体系： 10扩增缓冲液 10ul 4种dNTP混合物 各200umol/L
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Cloning PCR products using TA vectors
Cloning PCR products using TA vectorsby Paul N. Hengen, Ph.D. *Methods and reage
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Blunt end cloning of PCR products
End repair: Add 5-10 units of T4 DNApol and incubate at 37C for 5 minutes. Make
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Polymerase Chain Reaction (PCR) cont.
Polymerase Chain Reaction (PCR) cont. Choice of Polymerases for PCROne of the i
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提高PCR的忠实性
带有校正功能的酶包括克隆和效率分析，PCR产物表达或定点突变在内的PCR应用都需要高忠实性的PCR。Taq DNA聚合酶被看做是低忠实性的聚合酶，因为其缺少3&
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Ethanol precipitation method for purifying PCR products
1. For each PCR reaction (50 µL) prepare a 1.5mL microcentrifuge tube containing
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An Economic PCR Enhancer for GC-Rich PCR Templates
Since PCR is often problematic on GC-rich templates, we have evaluated different
