Immunoaffinity Identification of 2-DE Separated Proteins

First devised in a time dominated by one-dimensional SDS-PAGE, immunoblotting (also dubbed “Western” blotting, [1 ,2 ]), is now widely used in conjunction with 2-D PAGE (electrofocusing/SDS-PAGE), whose diffusion is favored by its long-awaited standardization (3 ,4 ) and by the coming of age of proteome science and technology (5 ,6 ).