质粒的大量制备

 Plasmid Mini and Maxi Prep Methods (Gimila Lab) 

・         Maxi-preps and all media, solutions (NWFSC) Isolation of cosmid, plasmid and P1 DNAs via diatomaceous earth-based Maxi, Midi and Mini-preps, and the non-diatomaceous earth-based Mini-prep.

• Large Scale Plasmid Preparation (Donis Keller Lab) For recovery of highly purified recombinant plasmid DNA using a CsCl2 gradient.   

・         Plasmid Maxiprep (Bowtell Lab)

・         Large scale double-stranded DNA isolation (Roe Lab) Provides protocol of isolating double-stranded DNA from plasmid or cosmid by the alkaline lysis procedure followed by equilibrium ultracentrifugation in cesium chloride-ethidium bromide gradients and diatomaceous earth-based purification method 

・         Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation (Roe Lab)

・         CsCl Prep. of Plasmid DNA (Mike A. Dyer) This is a standard large scale prep. for plasmid DNA which gives a yield of 0.5 - 1.0 mg.