Polymerase Chain Reaction

Materials:

Supplies: Procedures: Results:

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• PCR enzyme (5 Units/ul)
• PCR enzyme reaction buffer
• Distilled water (nuclease free)
• DNA to be used as template, such as GAPDH template from Clontech
• DNA to be used as upstream and downstream primers, such as the corresponding primers set for GAPDH template from Clontech
• Light mineral oil (nuclease free)
• dNTP mix (10 mM of each dNTP)
  • PCR machine and the 600-ul tubes for use in the machine
  • Micropipetter and tips
  • Microcentrifuge with adaptors for 600-ul tubes
• dNTP mix (10 mM of each dNTP)
  • PCR machine and the 600-ul tubes for use in the machine
  • Micropipetter and tips
  • Microcentrifuge with adaptors for 600-ul tubes