DNA甲基化分析
The influence of methylation on the promoter activity and gene expression and th
The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive research of methylation processes and it's influence on the genome functioning. Analysis of DNA methylation became a routine method during the last decade. I worked with methylation extensively during my PhD studies and tried several methods. Here I place examples of 3 main approaches for methylation analysis. All these methods I have used personally, therefore if you have any questions don't hesitate to contact me.
| Methylation analysis using restriction enzyme digestion |
Contributed by A. Gratchev
Protocol
Contributed by A.Gratchev
Protocol
Contributed by A.Gratchev
Protocol for 32 P labelled nucleotides
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