切除融合标签常用蛋白酶的酶切位点
Cleavage Sites Table for Fusion Proteins：PROTEASECOMPANYProtease CaptureThrombin
Cleavage Sites Table for Fusion Proteins：
| PROTEASE | COMPANY | Protease Capture |
| Thrombin Leu-Val-Pro-Arg�Gly-Ser | Amersham-Biosciences, Novagen, SIGMA, Roche | Benzamidine-Agarose |
| Factor Xa Ile-Glu/Asp-Gly-Arg� | Amersham-Biosciences, New England Biolabs, Roche | Benzamidine-Agarose |
| Enterokinase Asp-Asp-Asp-Asp-Lys� | New England Biolabs, Novagen, Roche | Trypsin Inhibitor-Agarose |
| TEV protease Glu-Asn-Leu-Tyr-Phe-Gln�Gly | Invitrogen – Life Technologies | Ni-NTA (6His recomb. TEV) |
| PreScission Leu-Glu-Val-Leu-Phe-Gln�Gly-Pro | Amersham-Biosciences | GSTrap for GST fusion enzyme |
| TAGZyme His-tag removal by Exoproteolytic Digestion | Qiagen | Ni-NTA (6His recomb. enzyme) |
| Intein Site dithiothreitol cleavage | New England Biolabs | DTT elimination by dialysis |
| HRV 3C Protease Leu-Glu-Val-Leu-Phe-Gln�Gly-Pro | Novagen | Ni-NTA (6His recomb. enzyme) |
| SUMO Protease recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO | Invitrogen – Life Technologies, LifeSensors | Ni-NTA (6His recomb. enzyme) |
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