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General Linear Models
This chapter presents the general linear model as an extension to the two-sample
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Chromatin Immunoprecipitation Technique for Study of Transcriptional Dysregulation in Intact Mouse B
Transcriptional dysregulation has emerged as an important pathologic mechanism u
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冰冻切片操作注意事项
首先是冰冻切片实验制备的注意事项:1、新鲜组织的制备组织尽可能新鲜、骤冷愈快愈好,才能避免冰晶,常用的骤冷剂有:①CO2气(-78℃)②丙酮干冰冷却的轻石油醚、
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High-Throughput Screen for Aromatic Hydroxylation
The oxidation of aromatic compounds is important in producing chemical intermedi
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Evaluation of Circulating Endothelial Precursor Cells in Cancer Patients
Results obtained from preclinical studies have shown that endothelial progenitor
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冰冻切片制片的影响因素
1、组织取材对制片的影响组织取材大小:组织块大小要适宜,冷冻切片机内切片刀移动的范围有一定的限制,超出其范围时,易致切片不全,一般情况下,冰冻切片的组织大小为1
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冰冻切片与石蜡切片的区别
首先我们来看看什么是石蜡切片和冰冻切片:石蜡切片:石蜡切片是组织学常规制片技术中最为广泛应用的方法。石蜡切片不仅用于观察正常细胞组织的形态结构,也是病理学和法医
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酵母遗传学方法11:PCR介导基因破坏法
PCR 介导基因破坏法 1. 反应混合物:5 μ l 10 Taq 缓冲液5 μ l 25mmol/L MgC
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什么是HE染色法?
那么HE染色为什么应用如此广泛,它的原理是什么呢?HE染色原理是易于被碱性或酸性染料着色的性质称为嗜碱性和嗜酸性;而对碱性染料和酸性染料亲和力都比较弱的现象称为
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联会(synapsis)
同源染色体配对称为联会,是在减数分裂的偶线期两条同源染色体侧面紧密相帖并进行配对的现象。联会染色体间的配对是专一性的, 可以同时发生在分散的几个点上。实际上,同
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Promoter Analysis in Zebrafish Embryos
Analysis of promoter regulation is a powerful strategy for the study of regulato
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HE染色实操注意事项
1、pH值调节染色时调节pH值很重要。如果组织块在福尔马林中固定时间长,组织酸化而影响细胞核着色。因此,要在自来水中冲洗时间长一些或在饱和碳酸锂水溶液中处理10
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In Situ Hybridization to Plant Tissues and Chromosomes
In situ hybridization is a basic method in modern plant cell and molecular biolo
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Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro
The CD8+ , CD8− , and plasmacytoid dendritic cell (DC) subtypes develop from pro
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HE染色常见问题与处理方法
1、切片在脱蜡后出现大片白色的斑点原因:(1)烤(烘)片温度太低,玻片在脱蜡前没有充分烤(烘)干;(2)切片在二甲苯停留时间不足;(3)二甲苯使用过久,造成脱蜡
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