SSC-Molecular Biology

Hi,Can you tell me what do "SSC" in a southern blot, how he act on DNA or in the protocole.

(excuse-me for my traduction, i never write in english)

-Sebela-

"SSC" is a abbreviation of sodium chloride and sodium citrate solution, which used to transfer nuclear acid from gel to membrane ( 20 *SSC or 10*SSC or 5*SSC with alkaline). In Roche's DIG system, they said that the high solt concentration SSC shows much efficiently than the others like valcuum or electrophoresis.

-zhongmindai-

DNA is transferred most efficiently to nitrocellulose as a sodium salt where 90% of the effective negative charge has been neutralized by sodium chloride/citrate. Good Luck.

-phdconsult-