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FAQs on Real-Time RT-PCR
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers ar
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Standard multiplex mixtures
Standard multiplex mixtures Over 75 primer pairs were chosen and a number of m
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THE FOUNDATION OF SUCCESSFUL RT IN SITU PCR
TABLE OF CONTENTS 1. Abstract 2. Introductory statement 3. The key preparatory s
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RT-PCR Analysis
RT-PCR AnalysisSolutions10X RT Buffer10X PCR Buffer100 mM Tris pH 9.0500 mM KCl1
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Making RNA probes for in situ hybridization
Make DNA templates via PCRDay 1It's preferable to start with constructs that
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PCR常见问题总汇(二）
克隆PCR产物 1)克隆PCR产物的最优条件是什么？ 最佳插入片段：载体比需实验确定。1：1（插入片段：载体）常为最佳比，摩尔数比1：8或8：1也行。应测定比值
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PURIFICATION OF PCR PRODUCTS WITH SEPHADEX
PURIFICATION OF PCR PRODUCTS WITH SEPHADEXPlace the sephadex measuring plate (Mu
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Protocol for competitive RT-PCR
For quantifying mRNA, we use a competitive RT-PCR protocol with internal standar
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Bisulfite Treatment of DNA
Bisulfite Treatment of DNAAdapted from Frommer et.al.*Dilute DNA (up to 2 mg) in
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定量RT-PCR （Quantitative RT-PCR）
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and a
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RT-PCR经验浅谈
做RNA病毒基因的RT-PCR成败的关键首先在于RNA模板的制备。本人三年前做过一个正链RNA病毒全基因组分段扩增，设计方案是将全基因组分成7个片段，0.6kb
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What is the highest temperature that reverse trascriptases can be us
Question What is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSC
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如何建立一个PCR实验室？
为了对以个特定序列进行PCR做重复检测,需要三个不同的区域,每一个区域的具体技术操作和试剂在下面详细列出.1、样品准备区 这个区域专门用作样品的准备，在制备和操
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PCR常见问题总汇(一）
PCR产物的电泳检测时间 一般为48h以内，有些最好于当日电泳检测，大于48h后带型不规则甚致消失。 假阴性，不出现扩增条带 PCR反应的关键环节有①模
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Microdeletion screening
Microdeletion screening One application of multiplex PCR is microdeletion screen
