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What is the highest temperature that reverse trascriptases can be us

2024-11-14 PCR技术 加入收藏
Question What is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSC

Question  

What is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSCRIPT can be used?   

Answer  

For RT-PCR, SUPERSCRIPT II RT can be used up to 50 C. MMLV can be used at temperatures up to 42 C. THERMOSCRIPT can be used at temperatures as high as 70 C (especially for amplicons expected to be 1 kb or less). For PCR products expected to be greater than 1 kb a maximum first strand synthesis temperature of 65oC is suggested. Be sure your first-strand primer anneals at the high temperature.


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