-
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELS
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall: 165 x 1
-
如何快速鉴定转化子DNA
DNA连接与转化快速细胞破碎法实验步骤1、挑取转化平板上的单菌接种于含相应抗生素的2ml LB中,37℃振荡培养至A590值为0.6～0.8.2、取1ml菌液至
-
Native gel electrophoresis（非变性电泳）
Native gel electrophoresis Under native PAGE conditions, polypeptides retain the
-
Two Dimensional Gel Electrophoretic Analysis for the Human Plasma Proteome
OverviewThis protocol is a detail description of the laboratory procedure in per
-
2 Dimensional Gel Electrophoretic Analysis for Chicken Egg
Overview This protocol is a detail description of the procedure in performin
-
ACIDIC-NATIVE GEL Protocol( For Basic Proteins (pI7.0))
USE ONLY FRESH GELSStock Solutions1) 1.5M Acetate-KOH pH 4.3 (48ml 1MKOH + 17.2m
-
琼脂糖电泳protocol
试剂: 试样20μl DNA ladder 琼脂糖 TAE缓冲液 上样缓冲液含0.5μg/ml EB的电泳缓冲液50TAE贮存液: T
-
PCR-SSCP的发展现状
概述随着分子生物学技术的发展，检测基因结构和突变的方法不断涌现。尤其是PCR技术问世以后，各种与PCR相结合的基因检测技术进一步推动了基因研究的发展。如不对称P
-
PCR-SSCP注意的事项
SSCP是一种快速、简便、灵敏的检测基因突变的方法，为了使SSCP达到最佳效果，应注意下列事项。1. 重复性影响SSCP重复性的主要因素为电泳的电压和温度。这两
-
PCR-SSCP原理和操作步骤
原理PCR-SSCP是1989年日本Orita等创建的筛查突变的新技术。它是一种简单、快速、经济的点突变筛查手段。PCR-SSCP技术的基本原理是PCR扩增后的
-
内切酶在PCR反应产物中的活性(Activity of Restriction Enzymes in a Primer Extensio
通常PCR反应以后还要对产物进行酶切才能进入下一步工作。为了方便起见，我们可以将内切酶直接加入到PCR反应产物中去，而省略了纯化产物的步骤。下表归纳了在PCR产
-
RT-PCR Analysis--详细的RT-PCR方法
Solutions10X RT Buffer10X PCR Buffer100 mM Tris pH 9.0500 mM KCl1% Triton X-1002
-
RT-PCR: The Basics
RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive t
-
What is the highest temperature 
QuestionWhat is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSCRI
-
Real-Time or Kinetic PCR
The DNA Facility houses the “real-time” or kinetic PCR instrument, the Applied B
