通用引物序列
M13 Forward(-20)5’d[GTAAAACGACGGCCAG]3’M13 Forward(-40)5’d[GTTTTCCCAGTCACGAC]3’M
M13 Forward(-20) | 5’d[GTAAAACGACGGCCAG]3’ |
M13 Forward(-40) | 5’d[GTTTTCCCAGTCACGAC]3’ |
M13 Reverse | 5’d[CAGGAAACAGCTATGAC]3’ |
T7 Promoter | 5’d[TAATACGACTCACTATAGGG]3’ |
T3 Promoter | 5’d[ATTAACCCTCACTAAAGGGA]3’ |
Sp6 Promoter | 5’d[GATTTAGGTGACACTATAG]3’ |
M13/pUC Reverse | 5’d[AGCGGATAACAATTTCACACAAGG]3’ |
M13/pUC Forward | 5’d[CCCAGTCACGACGTTGTAAAACG]3’ |