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PCR Amplification of DNA
Materials:sterile water10X amplification buffer with 15mM MgCl2-10 mM dNTP50 μM
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Single tube confirmation PCR protocol
The following colony PCR protocol has been designed to be performed in individua
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RFLP－－限制片段长度多态性
RFLP－－限制片段长度多态性限制性片段长度多态性（RFLP，Restriction Fragment Length Polymorphism） RFLP技
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PCR from Tissue
1.protocol(1)collect piece of tissue (e.g., pieces of a leaf or flower) in Eppen
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PCR基本实验方法（一）
Recommended Reagent Concentrations:Primers: 0.2 - 1.0 uMNucleotides: 50 - 200 uM
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PCR检测人COX病毒及其应用
PCR 检测人COX病毒及其应用 柯萨奇病毒(Coxsackie viruses简称Cox.病毒)是1948年Dalldorf等采用新生小鼠研究脊髓灰质炎病
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Protocol for mRNA amplification--RT-PCR实验过程
Isolate Total RNA using Qiagen midi kit (Cat#75142) ( see manufacturer's pro
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聚合酶链反应
聚合酶链反应(Polymerase Chain Reaction)简称ＰＣＲ，是一项在短时间内大量扩增特定的ＤＮＡ片段的分子生物学技术。开发史这项技术的发明人是
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PCR反应的五要素
1、引物引物是PCR特异性反应的关键，PCR 产物的特异性取决于引物与模板DNA互补的程度。理论上，只要知道任何一段模板DNA序列，就能按其设计互补的寡核苷酸链
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Calculating Concentrations for PCR
1. Primers:i) Oligonucleotide primers are generally supplied as "so many OD
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聚合酶链反应一单链构象多态性分析 (PCR-SSCP) 实
聚合酶链反应一单链构象多态性分析 (PCR -SSCP) 聚合酶链反应-单链构象多态性分析（Single Strand Conformation Poly
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克隆PCR产物问题与解答
1)克隆PCR产物的最优条件是什么？ 最佳插入片段：载体比需实验确定。1：1（插入片段：载体）常为最佳比，摩尔数比1：8或8：1也行。应测定比值范围。连接用5u
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Real Time PCR problem - different dilutions have the sam
I have been working with the Real-time PCR and have run into a brick wall. My st
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Equipment And Reagents For Real-Time PCR
In September 1993 Higuchi et al. published a report in the journal Biotechnology
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个体配子DNA序列的PCR分析
个体配子DNA 序列的PCR分析 前言 高等生物遗传图谱的构建依赖于选择性杂交后代的分析或者通过家系分析法来计 算连锁关系。对人类而言仅后者是可行的。使用长
