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MAPK signaling pathway - Homo sapiens (human)
细胞坏死(necrosis)
Basic Method for Direct Immunofluorescence Labeling
原代软骨细胞分离培养的总结
肿瘤细胞的培养
细胞培养基中常用氨基酸及其作用
溶酶体（lysosome）
人粒细胞-巨噬细胞集落刺激因子（GM-CSF）酶联免疫分析
血小板活化检测方法
电子显微镜的使用与细胞超微结构观察
lipofectamine 2000脂质体转染293ft细胞经验
果蝇的外部形态观察及饲养技术
转染实验需知
最全面的MTT相关技巧大汇总
细胞培养常见26个问题
单克隆抗体技术(monoclonal antibody technique)
如何选用特殊细胞系培养基？
细胞生长检测4：MTS检测法
Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination
Cyclic AMP- Dependent Protein Kinase Activity Ratio Assay
Coimmunoprecipitation Assay
Sperm Morphology Assessment Using Strict (Tygerberg) Criteria
The Perinucleolar Compartment (PNC): Detection by Immunohistochemistry
细胞生物基本方法：肿瘤细胞培养
大鼠III型胶原蛋白（COL-III）酶联免疫分析（ELISA）
同源异型突变(homeotic mutation)
植物原生质体的制备
DEAE-葡聚糖介导的转染步骤
TISSUE CULTURE ON COVERSLIPS
胰岛素受体(insulin receptor)
无血清培养基
Nature子刊上的akt pathway的信号通路图
人成纤维细胞生长因子23（FGF-23）酶联免疫分析
Freezing and Thawing of MEFs
Protocol for Aortic Ring Assay
吞饮作用(pinocytosis)
核糖体（ribosome）
酵母遗传学方法6：羧肽酶Y的平板鉴定
流式细胞仪之细胞凋亡检测的PI双染色法
Use of EIA to Measure MMPs and TIMPs
Apoptosis Induction
激素(hormone)
Meiotic Cytogenetics in Coprinus cinereus
Scaffolds for Tissue Engineering and 3D Cell Culture
PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION (2 METHODS)
AKT信号通路概述
新的高内涵成像系统acumen® hci隆重上市
无水氯化镁黑色液体的特性及应用解析
细胞核(nucleus)
Studying Amino Acid Transport Using Liposomes
从G蛋白偶联受体到MAPK的通路图
Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes
Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Prot
The Production of Monoclonal Antibodies to Membrane Proteins
Use of Liposomes to Study Cellular Osmosensors
质体转染293细胞后细胞漂浮解答
姐妹染色单体分化染色方法
人中性粒细胞弹性蛋白酶（NE）酶联免疫分析（ELISA）
细胞凋亡形态学检测与观察
Preparing chemically competent cells
Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Location
Identification of Target Genes of a Yeast Transcriptional Repressor
Analysis of Skeletal Muscle Development in Drosophila
Studies of Adult Neural Stem Cell Migration
Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays
胸腹水染色体标本制备
Approaches to Cloning of Pain-Related Ion Channel Genes
ES Cell-Mediated Conditional Transgenesis
细胞培养的微生物污染排除
Imaging Lipid Bodies Within Leukocytes with Different Light Microscopy Techniques
小白鼠染色体的制备和观察
交换
Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors
RNA Extraction From Drosophila Heads
Linearization and Purification of the Shuttle Plasmid
Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extract in Semi-Intact MDCK Cells
内膜系统（endomembrane system）
Detection of Tight Junction Barrier Function In Vivo by Biotin
Single Cell Analysis of Lipid Rafts
Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes
Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells
Isolation and Culture of Primary Human Hepatocytes
Expression of Recombinant Membrane-Type MMPs
Computational Prediction of RNA Structural Motifs Involved in Post-Transcriptional Regulatory Proces
Identification of Urine Organic Acids for the Detection of Inborn Errors of Metabolism Using Urease
Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta
Purification Through Affinity Chromatography and Microsequencing
Local Application of Antisense for Prevention of Restenosis
小泡运输(transport by vesicles)
前中期(prometaphase)
The Measurement of Nitric Oxide and Its Metabolites in Biological Samples by Ozone-Based Chemilumine
Protein Degradation Via the Proteosome
Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans
Effects of Temperature Generated from the Holmium: YAG Laser on Human Osteoblasts in Monolayer Tissu
Beta-gal staining of eukaryotic cells in vitro
Assays of MSCs with Microarrays
Oligonucleotide FISH Probes
Detecting Tagged Hedgehog with Intracellular and Extracellular Immunocytochemistry for Functional An
动物细胞大规模微载体培养技术
Transforming chemically competent cells
The Analysis of tRNA Import Into Mammalian Mitochondria
Quantitation of Phencyclidine (PCP) in Urine and Blood Using Gas Chromatography-Mass Spectrometry (G
Comparison of lysis methods
Amniote Phylogenomics: Testing Evolutionary Hypotheses with BAC Library Scanning and Targeted Clone
Application of Adenoviral Vectors: Analysis of Eye Development
Interspecific Chimeras: Transplantation of Neural Crest Between Mouse and Chick Embryos
细胞生物基本方法：冻存
The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies
Pharmacogenomics of Membrane Transporters: An Overview
Ⅱ型糖原贮积症(glycogen storage disease type Ⅱ)
动物细胞培养实验室必备仪器
Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos
In Vitro Generation of Megakaryocytes and Platelets from Human Embryonic Stem Cells and Induced Plur
Notochord Grafts
An Assay to Monitor the Membrane Integration of Single-Span Proteins
Spectral Bio-Imaging and Confocal Imaging of the Intracellular Distribution of Lipoplexes
Ex Vivo Megakaryocyte Expansion and Platelet Production from Human Cord Blood Stem Cells
细胞生理学(cytophysiology)
基因导入细胞的常用方法
Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence
Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells
Quantification of Cell Fusion by Flow Cytometry
Analysis of Unstable Triplet Repeats Using Small-Pool Polymerase Chain Reaction
Proteomics Studies After Hematopoietic Stem Cell Transplantation
A Functional Genomics Approach to Identify and Characterize Oxidation Resistance Genes
Progress Toward a Synthetic Virus: A Multicomponent System for Liver-Directed DNA Delivery
Hematopoietic Colony-Forming Cell Assays
In Vitro Two-Dimensional Endothelial Differentiation of Human Embryonic Stem Cells
Subculture of Suspension Cell Lines
Triplet Repeats and DNA Repair: Germ Cell and Somatic Cell Instability in Transgenic Mice
Characterization of Megakaryocyte Development in the Native Bone Marrow Environment
Activity-Based Protein Profiling of Protein Tyrosine Phosphatases
Mismatch Repair Assay
Color-Changing Karyotyping (CCK), an M-FISH/SKY Alternative
Cell Death in Myoblasts and Muscles
Cryopreservation of Seeds
Hematopoietic Development of Human Embryonic Stem Cells in Culture
纤毛动力蛋白(ciliary dynein)
副节(parasegment)
Electroporation of Drosophila Embryos
Directed Evolution of the Substrate Specificities of a Site-Specific Recombinase and an Aminoacyl-tR
人白细胞介素32γ(IL-32γ)酶联免疫分析（ELISA）
Comprehensive Hematopoietic Stem Cell Isolation Methods
生物显微摄影(Microphotography)
细胞分化的调节
正常细胞与肿瘤细胞常规核型的标本制备
Measurement of Neutrophil Elastase, Proteinase 3, and Cathepsin G Activities using Intramolecularly
Investigating Microtubule Dynamic Instability Using Microtubule-Targeting Agents
Induced Pluripotent Stem Cells (iPSC) from Cord Blood CD133+ Cells Using Oct4 and Sox2
Development of Immortalized Hepatocyte-Like Cells from hMSCs
Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants
Primary Culture and Differentiation of Human Adipocyte Precursor Cells
DNA Damage Quantitation by Alkaline Gel Electrophoresis
Patch-Clamping Drosophila Sensory Neurons
In Vivo Quantitative Proteomics: The SILAC Mouse
A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors
Nonclinical and Clinical Experiences with CPP-Based Self-Assembling Peptide Systems in Topical Drug
Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice
Assisted Reproductive Technology in Nonhuman Primates
Imaging of Intracellular Hydrogen Peroxide Production with Hyper Upon Stimulation of Hela Cells with
Methods for the Purification of HQ-Tagged Proteins
Culturing HEK 293 Cells
细胞生物基本方法：复苏
Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions
High Resolution Fluorescent In Situ Hybridization in Drosophila
An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells
Cultures of Human Adipose Precursor Cells
Primary Mouse Keratinocyte Culture
Gene Expression in Mouse Oocytes by RNA-Seq
Localization of mRNA in Vertebrate Axonal Compartments by In Situ Hybridization
The In Vivo Evaluation of Active-Site TOR Inhibitors in Models of BCR-ABL+ Leukemia
Analysis of Small Hsp Phosphorylation
Electrical Impedance Spectroscopy for Rapid and Noninvasive Analysis of Skin Electroporation
Preparation of Segmented and Polarity Marked Microtubules
Chemotaxis in Neutrophil-Like HL-60 Cells
Immunolocalization in the Epidermis
In Vivo Analysis of Ribonucleoprotein Complexes Using Nucleotide Analog Interference Mapping
Noncovalent Protein Interaction with Poly(ADP-Ribose)
Expression Profiling Using Quantitative Hybridization on Macroarrays
Isolation of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammali
Methylated Polyamines as Research Tools
Subcellular Fractionation of Adipocytes and 3T3-L1 Cells
Manipulating Claudin Expression in Avian Embryos
Deriving Metabolically Active Hepatic Endoderm from Pluripotent Stem Cells
Antisense Tumor Therapy: Activated C-Ha-ras Oncogene in the Mouse
Immunocytochemical Analysis of Human Pluripotent Stem Cells
Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells
Antisense Strategies for Redirection of Drug Metabolism: Using Paclitaxel as a Model
滤泡细胞(follicle cell)
大鼠血小板活化因子受体（PAFR）酶联免疫分析（ELISA）
Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts
Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells
Instrumentation and Electrodes for In Vivo Electroporation
Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes
Recording Single-Channel Currents Using Smart Patch-Clamp Technique
Identifying the Spatial Distribution of Vitamin E, Pulmonary Surfactant and Membrane Lipids in Cells
膜(membrane)
Retroviral Transduction of Hematopoietic Progenitors Derived From Human Embryonic Stem Cells
Multifluorescence Labeling and Colocalization Analyses
Site-Directed Mutagenesis Using Positive Antibiotic Selection
The Coculture Method to Examine Interactions Between Claudin Isoforms in Tight Junction-Free HEK293
Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spi
Monitoring Patterns of Gene Expression in Dictyostelium by -galacotsidase Staining
Skin Morphogenesis: Embryonic Chicken Skin Explant Cultures
Labeling and Purification of RNA Synthesized by In Vitro Transcription
Neural Differentiation of Embryonic Stem Cells
A rapid and highly sensitive method for somatic mutation profiling using the Sequenom MassARRAY® Sys
Gene Transfer into Cystic Fibrosis Airway Epithelial Cells
Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies
Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival
Protoplast Transformation of Filamentous Fungi
Purification of Peripheral Blood Natural Killer Cells
Detection of Microsatellite Instability in Cancers by Means of Nongel-Sieving Capillary Electrophore
ES Cell Culture and Manipulation
Peptide Nanofiber Scaffolds for Multipotent Stromal Cell Culturing
Use of Polyamine Metabolites as Markers for Stroke and Renal Failure
Plastic dishes for growing cells
Simultaneous Fluorescence Immunostaining and FISH
The Basis of Electrochemotherapy
Isolation and Functional Analysis of Mitochondria From the Nematode Caenorhabditis elegans
ATP Production in Isolated Mitochondria of Procyclic Trypanosoma brucei
Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics)
Liposomes for Specific Depletion of Macrophages from Organs and Tissues
Isolation of a Sequence-Specific RNA Binding Protein, Polypyrimidine Tract Binding Protein, Using RN
Using Schizosaccharomyces pombe Meiosis to Analyze DNA Recombination Intermediates
How to Assess and Study Cell Death in Dictyostelium discoideum
Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic
Ultrastructural Imaging of Cell Fusion in Caenorhabditis elegans
Proteomics Method for Identification of Pseudopodium Phosphotyrosine Proteins
MITOMYCIN C TREATMENT OF PMEFs
Quantitation of Orotic Acid in Urine Using Isotope Dilution-Selected Ion Gas Chromatography-Mass Spe
Identification of Specific Protein-RNA Target Sites Using Libraries of Natural Sequences
Characterization of Human Adipose-Derived Stem Cells Using Flow Cytometry
Microalgal Immobilization Methods
In Vitro Selection of Aptamers from RNA Libraries
Antioxidant QSAR Modeling as Exemplified on Polyphenols
Mesenchymal Cell Fusion in the Sea Urchin Embryo
Photoorientation in Photosynthetic Flagellates
Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistoche
Production of Chimeric Chickens
Electrochemotherapy: Animal Model Work Review
Micro-injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes
Panel Development for Multicolor Flow-Cytometry Testing of Proliferation and Immunophenotype in hMSC
Culture Technique of Human Pituitary Adenoma Cells
Detection of Expression Patterns in Hydra Pattern Formation
Culture Systems: Physiological and Environmental Factors That Can Affect the Outcome of Human ART
Embryo Culture and Epigenetics
Gene Expression in Polytene Nuclei
SopE-Mediated Recruitment of Host Rab5 on Phagosomes Inhibits Salmonella Transport to Lysosomes
Mapping Cis- and Trans- Chromatin Interaction Networks Using Chromosome Conformation Capture (3C)
Combined Peptidomics and Genomics Approach to the Isolation of Amphibian Antimicrobial Peptides
Using Drosophila S2 Cells to Measure S phase-Coupled Protein Destruction via Flow Cytometry
PTDDRBD siRNA Delivery
细胞培养中的黑胶虫问题
Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins
Isolation, Purification, and Growth of Human Skeletal Muscle Cells
Live Imaging Mouse Embryonic Development: Seeing Is Believing and Revealing
Promoter Analysis of TGF-Responsive Genes by Transient Transfection and Deletional/Mutational Analys
Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for
Induction of Ca2+ Flux by Adhesion Molecules in Lymphocytes
Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxyg
Determination of the Clinical Significance of an Unclassified Variant
A Live Cell Micro-imaging Technique to Examine Platelet Calcium Signaling Dynamics Under Blood Flow
DNA laddering assay for treated cells
Detecting Recruitment of DNA Damage Response Factors Through the eChIP Approach
Transfection and Promoter Analysis in Embryonic Stem Cells
Methods to Analyze Transglutamination of Proteins Involved in Apoptosis
Scanning for Transcription Factor Binding by a Variant EMSA
A Rapid and Selective Mass Spectrometric Method for the Identification of Nitrated Proteins
Analysis of Tissue Interactions in Ectodermal Organ Culture
Use of Repetitive Element Palindromic PCR (rep-PCR) for the Epidemiologic Discrimination of Foodborn
Antioxidant Activity of Biotransformed Sex Hormones Facilitated by Bacillus stearothermophilus
The Mycobacterium tuberculosis Phagosome
活体成像中荧光色素标记细胞的方法举例
Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture
Bioluminescence Imaging of Bone Metastasis in Rodents
Post-transplant Monitoring of Chimerism by Lineage-Specific Analysis
Analyzing Cell Death Events in Cultured Leukocytes
一氧化氮、总抗氧化能力对大鼠隐睾生殖细胞凋亡的影响
293细胞的大规模培养
Detection and Analysis of (O-linked -N-Acetylglucosamine)-Modified Proteins
Application of Cell Traction Force Microscopy for Cell Biology Research
The Dansyl-Edman Method for Peptide Sequencing
Cotransfection Assays and Steroid Receptor Biology
ECM Macromolecules: Rotary Shadowing and Scanning Transmission Electron Microscopy
Culture Systems: Embryo Culture and Monozygotic Twinning
Isolation of Membrane Rafts and Signaling Complexes
Alkaline Phosphatase (EC 3.1.3.1)
内含子(intron)
Enhanced Cellular Delivery of Cell-Penetrating PeptidePeptide Nucleic Acid Conjugates by Photochemic
A Dot Blot Immunoassay for UV Photoproducts
Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro
In Vitro Histone Demethylase Assays
Methods for Functional Analysis of Stem Cells
Mutation Detection in the TGF Receptors and SMAD Genes: RT-PCR and Sequencing
Human Pluripotent Stem Cells: The Development of High-Content Screening Strategies
Environment-Responsive Multifunctional Liposomes
Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs
Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin
Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs
Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin
Detection and Characterization of Receptor Interactions with PDZ Domains
Investigating Myc-Dependent Translational Regulation in Normal and Cancer Cells
Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
Chromatophores as Tools for the Study of Organelle Transport
Reliable Method for Detection of Programmed Cell Death in Yeast
Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos
Hematopoietic Stem Cell Mobilization: A Clinical Protocol
Fibrin Gel Model for Assessment of Cellular Contractility
Discrete Dynamic Modeling of Signal Transduction Networks
Quantification of Antidepressants Using Gas Chromatography-Mass Spectrometry
Isolation, Culture, and Osteogenic/Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal S
RIP: An mRNA Localization Technique
Crystallization of Membrane Proteins
Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteins
Peptidomics Analysis of Lymphoblastoid Cell Lines
Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells
电位-门控通道(voltage-gated channels)
“夸娥”因子的作用介绍
Inhibition of PI3K-Akt-mTOR Signaling in Glioblastoma by mTORC1/2 Inhibitors
Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand
In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Character
Revealing the Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling Microarray
Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern
Quality Control Considerations
Purification of the Ndc80 Kinetochore Subcomplex From Xenopus Eggs
In Vitro Genetic Analysis of RNA-Binding Proteins Using Phage Display
Human Embryonic Stem (ES) Cell Protocols――General notes on ES cell culture
Endothelial wound healing (cell migration) assay
Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues
Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent
Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications
Aminopeptidases: Aminopeptidase M (EC 3.4.11.2), Pyroglutamate Aminopeptidase (EC 3.4.19.3), and Pro
Chicken Embryonic Stem Cells: Establishment and Characterization
Current Trends in Liposome Research
Inflammation and Wound Healing in Drosophila
Utility of Animal Models for Human Embryo Culture Development: Rodents
Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview
Analyzing the Developmental Expression of Sigma Factors with S1-Nuclease Mapping
Pooling Data When Analyzing Biomarkers Subject to a Limit of Detection
Developmental Control of Growth and Cell Cycle Progression in Drosophila
细肌丝(thin filament)
Isolating RNA with the Cationic Surfactant, Catrimox-14
Use of Necrotic Markers in the Drosophila Ovary
细胞实验原创经验
Screening for Mutations in Cartilage ECM Genes
Pronuclear Microinjection of Mouse Zygotes
分子内伴侣(intramolecular chaperones)
大鼠内皮型NO合酶(eNOS）酶联免疫分析（ELISA）
Biochemical Analysis of Claudin-Binding Compatibility
Cryopreservation of Human Gametes
Ovariectomy/Orchidectomy in Rodents
Biologically Based Risk Assessment Models for Developmental Toxicity
Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids
Polo-Like Kinase-1: Activity Measurement and RNAi-Mediated Knockdown
Gene Expression Analysis by In Situ Hybridization: Radioactive Probes
Cryofixation Methods for Ultrastructural Studies of Dictyostelium discoideum
Retroposons: Genetic Footprints on the Evolutionary Paths of Life
Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors
Visualization of Complex Processes in Lipid Systems Using Computer Simulations and Molecular Graphic
The Use of RNA Probes for the Analysis of Gene Expression: Northern Blot Hybridization and Ribonucle
Directed Differentiation of Dopamine Neurons from Human Pluripotent Stem Cells
Lineage Selection for Generation and Amplification of Neural Precursor Cells
Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity
Analysis of Skeletal Muscle Hypertrophy in Models of Increased Loading
Crystallization and Crystal Structure Determination of Ribonuclease A-Ribonuclease Inhibitor Protein
Lipidomic Analysis of Human Meibum Using HPLCMSn
The Use of RAPD for Isolate Identification of Arbuscular Mycorrhizal Fungi
Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells
Colony Cultures: Plating Efficiency Assay and Cloning
Preparation of Feeder Layers And SNL Stocks
In Vivo Transplantation of Genetically Modified Mouse Embryonic Epidermis
Restriction Fragment Length Polymorphism (RFLP) Analyses: Distinguishing African and European Honey
Calcium and Membrane Repair
The Yeast Three-Hybrid System As a Tool to Study Caspases
Freezing Harvested hMSCs and Recovery of hMSCs from Frozen Vials for Subsequent Expansion, Analysis,
细胞生物学名词解释
Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular Membranes
Analysis of the Enantiomers of Chiral Pesticides and Other Pollutants in Environmental Samples by Ca
Isolation of Centrosomes from Cultured Cells
Development and Evaluation of Classifiers
Generation of Lung Epithelial-Like Tissue from hESC by AirLiquid Interface Culture
Humanized Mice for Studying Human Leukocyte Integrins In Vivo
Labeled Stem Cells as Disease Models and in Drug Discovery
Chromosomal Mosaicism in Neural Stem Cells
Expression of Epitope-Tagged Proteins in Arabidopsis Leaf Mesophyll Protoplasts
染色体分带(chromosome banding)
Light-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors
spa Typing for Epidemiological Surveillance of Staphylococcus aureus
ght-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors
Performing Comparative Peptidomics Analyses of Salmonella from Different Growth Conditions
Use of Stem Cell Markers in Dissociated Mammary Populations
Methods for Culture of Human Corneal and Conjunctival Epithelia
Transfection and Transduction of Primary Human Endothelial Cells
A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Prese
Flow Cytometry-Based Apoptosis Detection
Subculture of Adherent Cell Lines
Quantitation of Opioids in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)
Serum Apolipoproteins
Antisense Therapeutics for Hypertension: Targeting the Renin-Angiotensin System
In Vitro Phosphorylation and Kinase Assays in Neurospora crassa
抑郁症的体外模型
Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone
Cell-Penetrating Penta-Peptides and Bax-Inhibiting Peptides: Protocol for Their Application
人抗胰岛细胞抗体(AICA)酶联免疫分析（ELISA）
细胞培养过程中常见问题及其解答
Analysis of Point Mutations by Use of Amber Stop Codon Suppression
High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed
Manufacture of Clinical Grade Human Placenta-Derived Multipotent Mesenchymal Stromal Cells
Microarray Analysis
Measuring DNA Precursor Pools in Mitochondria
Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly
Interface Organotypic Hippocampal Slice Cultures
Freezing Cells
肌纤维(myofibers)
Mutagenesis With Drosophila
RNA Extraction From Mammalian Tissues
PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes
Tissue-Selective Knockouts of Steroid Receptors: A Novel Paradigm in the Study of Steroid Action
绒毛染色体制备方法
FISH on Plant Chromosomes
Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotides
Computational Reconstruction of Ancestral DNA Sequences
Characterization of Genes for Polyamine Modulon
Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture
Assessing Sequence-Specific DNA Binding and Transcriptional Activity of STAT1 Transcription Factor
Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells
T Cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T Cells in Chronic Inte
Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis
Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications
Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay
Assessment of Apoptotic Cell Phagocytosis by Macrophages
Mapping Protein-DNA Interactions Using In Vivo Footprinting
Assessment of Apoptotic Cell Phagocytosis by Macrophages
Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay
Strategies for Imaging Microtubules in Plant Cells
Eradication of Mycoplasma Contaminations
Exploring Polyamine Biosynthetic Diversity Through Comparative and Functional Genomics
Immunocytochemical Techniques for Studying Apoptosis in Primary Sympathetic Neurons
后期(anaphase)
Isolation and In Vitro Binding of Mating Type Plus Fertilization Tubules From Chlamydomonas
Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides
Humanized Mouse Models to Study the Human Haematopoietic Stem Cell Compartment
Handmade Somatic Cell Cloning in Cattle
Drosophila melanogaster and the Development of Biology in the 20th Century
A Simple Crosslinking Method, CLAMP, to Map the Sites of RNA-Contacting Domains Within a Protein
RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells
Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium
Assay of Deoxyhypusine Synthase Activity
核孔运输(transport through nuclear pore)
Electrotransfection with Intracellular Buffer
Multidimensional Proteomic Analysis of Proteolytic Pathways Involved in Cell Cycle Control
Effects of Rho Family GTPases on Cell-Cell Adhesion
中心体
Modulation of Potassium Ion Channel Proteins Utilising Antibodies
Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation
Purification of Murine Monoclonal Antibodies
Characterization of Enzyme Activity, Protein Content, and Thiol Groups in Immobilized Enzymes
Oocyte Isolation and Enucleation
Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein
Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization
Detection of Apoptosis in the Central Nervous System
Serum-Resistant Lipoplexes in the Presence of Asialofetuin
Isolation and Characterization of Primitive Hematopoietic Cells Based on Their Position in the Cell
Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading
Epithelial-Mesenchymal Interactions
自由基(free radical)
DNA Microarray for Molecular Epidemiology of Salmonella
Nuclear Reprogramming of Somatic Nucleus Hybridized With Embryonic Stem Cells by Electrofusion
Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis
液泡(vacuoles)
Generation of Transgene-Free iPSC Lines from Human Normal and Neoplastic Blood Cells Using Episomal
Developmental Biology Protocols: Overview I
Immunogold Labelling for Scanning Electron Microscopy
Human Long-Term Culture Initiating Cell Assay
Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Li
Electroporation Strategies for Genetic Manipulation and Cell Labeling
Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development
Primary Marrow-Derived Stromal Cells: Isolation and Manipulation
Transplantation of Human Embryonic Stem Cells to the Chick Embryo
Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography,
缝隙连接（gap junction）
Microinjection into Xenopus Oocytes and Embryos
M-Current Recording from Acute DRG Slices
前质体(proplastid)
The Problems and Pitfalls of Radioligand Binding
Differentiation of B Lymphocytes From Hematopoietic Stem Cells
贴壁细胞培养过程中支原体污染的几种救治方案
High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation
Disrupting Protein Complexes Using Tat-Tagged Peptide Mimics
细胞培养目的与用途
细胞培养器械的清洗与消毒方法
细胞生物基本方法：肌组织细胞培养
Autophagy in Disease
Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research
Mammalian In Vitro Splicing Assays
核剪接(nuclear splicing)
Creating a Hopeful Monster: Mouse Forward Genetic Screens
猴白细胞介素-4（IL-4）酶联免疫分析（ELISA）
Activation of p38 and Determination of Its Activity
细胞培养基的基本成分
Cell and Tissue Transplantation in Zebrafish Embryos
Direct PCR Sequencing with Denaturants (Formamide)
Long-Term B Lymphoid Cultures from Murine Bone Marrow: Establishment and Cloning by Using Stromal Ce
Generation of Inner Ear Cell Types From Embryonic Stem Cells
Fluorescent Biosensors for the Detection of HMGB1 Release
Media Composition: Amino Acids and Cellular Homeostasis
Overview
Light Microscopy to Image and Quantify Cell Movement
Culture of BEND Cells (Bovine Endometrial Cells)
乳制品中激素的快速检测
线粒体膜蛋白与细胞凋亡
Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Embryos Usi
Methods to Detect Apoptotic-Like Cell Death in Filamentous Fungi
Methods to Analyze the Homing Efficiency and Spatial Distribution of Hematopoietic Stem and Progenit
Immunolocalization of Poly ADP-Ribose on Drosophila Polytene Chromosomes
Hematopoietic Stem Cell Mobilization with G-CSF
Incorporation of Genetically Modified Cells in Chicken Chimeras
Quantitative Assessment of Pheromone-Induced Dauer Formation in Caenorhabditis elegans
Tube Formation: an In Vitro Matrigel Angiogenesis Assay
Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID
Large-Scale Mitotic Cell Synchronization
Cartilage Proteoglycan Release Assay
Isolation of Song-Regulated Genes in the Brain of Songbirds
Chick Embryos: Incubation and Isolation
Identification of Neural Stem Cells in the Drosophila Larval Brain
转细胞运输(transcellular transport)
Immunogold Labeling for Electron Microscopy
Lentiviral Gene Transduction of Mouse and Human Stem Cells
Application of Functional Blocking Antibodies: N-Cadherin and Chick Embryonic Limb Development
The Mammalian Cell Cycle: An Overview
Ultrastructural Analysis of Myoblast Fusion in Drosophila
Determination of Fatty Acid Profiles and TAGs in Vegetable Oils by MALDI-TOF/MS Fingerprinting
Manual Methods for Sperm Motility Assessment
Identification and Characterization of Programmed Cell Death Markers in Bacterial Models
Preparation of Substrata for In Vitro Culture of Neurons
Cloning Differentially Expressed Genes by Using Differential Display and Subtractive Hybridization
Genomic Amplification with Transcript Sequencing (GAWTS)
Chondrogenic Differentiation of Menstrual Blood-Derived Stem Cells on Nanofibrous Scaffolds
MAPK Signaling Pathway-Aviva
Claudin-16/Paracellin-1, Cloning, Expression, and Its Role in Tight Junction Functions in Cancer and
Protein Extraction From Drosophila Heads
Defrosting Eukaryotic Cells Frozen In Liquid N2
形态建成(morphogenesis)
Live Imaging of Bone Cell and Organ Cultures
The Molecular Identification of Trypanosomes
Epigenetic Control in Male Germ Cells: A Transillumination-Assisted Microdissection Method for the A
Expanding Human T Regulatory Cells with the mTOR-Inhibitor Rapamycin
Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-P
The Use of In Vivo Microdialysis Techniques to Detect Extracellular ROS in Resting and Contracting S
Investigations of Neuronal Migration in the Central Nervous System
Integrins and Vascular Development in Differentiated Embryonic Stem Cells In Vitro
Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders
Protein Extraction, Fractionation, and Purification From Cyanobacteria
Predicting Riboswitch Regulation on a Genomic Scale
Whole Cell Entrapment Techniques
Acid-Labile Liposome/pDNA Complexes
Lentiviral Vector-Mediated Transgenesis in Human Embryonic Stem Cells
Purification of PCR Products from Agarose Gels for Direct Sequencing
水稻根尖染色体标本制备
Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography
Ex Vivo Expansion of Haematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation
Sequencing of Antibodies
How do I decontaminate my tissue culture
Peptide-Induced Fusion of Liposomes
Modeling Spatial and Temporal Dynamics of Chemotactic Networks
Site-Specific Cleavage of Fusion Proteins
Preparation of Low Density, Collagenase-Digested Splenocytes (Hedrick Lab, UCSD Cancer Center)
A Practical Guide for using the AdEasy System
Transfection of Osteoclasts and Osteoclast Precursors
Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules
Method: Reactivating Cell Lines and Cell Growth for DNA Preparation
Site-Directed Mutagenesis in the Study of Membrane Transporters
Analysis of Proteins from Marine Molluscs
The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Huma
The Use of Calorie Restriction Mimetics to Study Aging
Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures
MMP-9 and TIMP-1 Assays in Keratinocyte Cultures
Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues
PKA系统(protein kinase A system, PKA)
Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems
Measurement of Caspase Activity in Cells Undergoing Apoptosis
Cell Fusion Assays for Yeast Mating Pairs
Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis
Flow Cytometric Analysis of Human Pluripotent Stem Cells
核糖体(ribosome)
Quantifying Hematopoietic Stem and Progenitor Cell Mobilization
Capillary Electrophoresis Separation of Microorganisms
几种常用的细菌计数方法
Protein Expression and Purification of Integrin I Domains and IgSF Ligands for Crystallography
Cell Fusion in Caenorhabditis elegans
Single Channel Analysis of Membrane Proteins in Artificial Bilayer Membranes
ERK-MAP Kinase Signaling in the Cytoplasm
Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics
Adhesion, Migration, and Homing of Murine Hematopoietic Stem Cells and Progenitors
High Sensitivity Measurement of Estrone and Estradiol in Serum and Plasma Using LC-MS/MS
Silencing in Yeast: Identification of Clr4 Targets
Use of the Hollow Fibre Assay for Studies on Tumor Neovasculature
Knockout Mice and Steroid Receptor Research
Ultrastructural Analysis of Cell Fusion in Yeast
Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry
A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-cultures
Monitoring of Mycophenolate Acid in Serum or Plasma Using LC Tandem Mass Spectrometry
Analysis of Changes in Protein Level and Subcellular Localization During Cell Cycle Progression Usin
Purification of Recombinant Poly(ADP-Ribose) Polymerases
de Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination
3D Sample Preparation for Orthopaedic Tissue Engineering Bioreactors
Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing
Efficient Gene Knockdowns in Human Embryonic Stem Cells Using Lentiviral-Based RNAi
Toxicities of Mobilized Stem Cell Infusion
光吸收(light absorption)
Analysis of Phospholipids by High Performance Liquid Chromatography
Isolation and Culture of Primary Chicken Osteoclasts
Gene Transfer Via Nucleofection Into Adult and Embryonic Stem Cells
Immunoprecipitation Analysis to Study RNA-Protein Interactions in Xenopus Oocytes
Proteomic Analysis of Dystrophic Muscle
人末梢血微量全血培养染色体显示法
The Myc World Within Reach
Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic M
Preparative Electrophoresis: Extraction of Nucleic Acids From Gels
The 25A/RNase L Pathway and Inhibition by RNase L Inhibitor (RLI)
Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display
Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease
细胞计数实验中初学者易犯的错误
Primary Culture of Mammalian Taste Epithelium
Whole-Cell Recording Using the Perforated Patch Clamp Technique
Focal Cerebral Ischemia
Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties
Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs
Treatment of Human Pancreatic Tumors Xenografted in Nude Mice by Chemotherapy Combined with Pulsed E
Retroviral Insertion Site Analysis in Dominant Haematopoietic Clones
Enhancer Analysis of the 1(II) and 2(XI) Collagen Genes in Transfected Chondrocytes and Transgenic M
Derivation of Neural Precursors from Bovine Preimplantation Embryos
Gene Transfer Using Targeted Filamentous Bacteriophage
氧化酶(oxidase)
Soft Tissue Reconstruction
Preparation of Fungi for Ultrastructural Investigations and Immunogoldlabelling
pH Sensing Agar Plate Assays for Esterolytic Enzyme Activity
ECM Macromolecules: Height-mapping and Nano-mechanics Using Atomic Force Microscopy
Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplat
电穿孔法进行质体转化步骤
Isolation of Porcine Pancreatic Islets for Xenotransplantation
Production and Purification of Polyclonal Antibodies
High-Resolution Molecular Localization by Freeze-Fracture Replica Labeling
An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue
Assessment of Cardiac Conduction: Basic Principles of Optical Mapping
Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitro
G-蛋白偶联受体(G-protein linked receptor)
Transfection of Adipocytes and Preparation of Nuclear Extracts
Utility of Array CGH in Molecular Diagnosis of Mitochondrial Disorders
Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resin
Simple Experimental and Spontaneous Metastasis Assays in Mice
Functional Genomics by Gene-Trapping in Embryonic Stem Cells
原生质体(potoplast)
Development of ATP-Competitive mTOR Inhibitors
Detecting Expression Patterns of Wnt Pathway Components in Sea Urchin Embryos
Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes
The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins
Measuring Redox Changes In Vivo in Leaves: Prospects and Technical Challenges
Quantitation of Benzodiazepines in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS
A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor
FISH Analysis of Human Pluripotent Stem Cells
Immobilized Metal Ion Affinity Chromatography of Native Proteins
Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction
Measurements of the Intracellular Stability of CPPs
Isolation of Skin Dendritic Cells from Mouse and Man
Application of Fluorescent Measurements for Characterization of RiboswitchLigand Interactions
MicroRNA Knock Down by Cholesterol-Conjugated Antisense Oligos in Mouse Organ Culture
Colorimetric Assays for Screening Laccases
Site-Specific Integration in Human ESC Using Jump-In TI Technology
Preparation and Testing of Synthetic mRNA for Microinjection
Mobilization of Hematopoietic Stem and Progenitor Cells in Mice
Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds
Time-Lapse Imaging of Necrosis
Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from
Transmission Electron Microscopy of Bone
Immune Privilege of Stem Cells
Investigation of Nuclear Envelope Structure and Passive Permeability
Isolation, Identification, and Validation of Microtubule-Associated Proteins from Drosophila Embryos
细胞融合技术的应用研究进展
Assessment of Permeability Barriers to Macromolecules in the Rodent Endometrium at the Onset of Impl
Analysis of the Budding Yeast Saccharomyces cerevisiae Cell Cycle by Morphological Criteria and Flow
Chemical Analysis of Aquatic Pheromones in Fish
Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets
Detection of Impending Graft Rejection and Relapse by Lineage-Specific Chimerism Analysis
Capillary Electrophoresis of Oxidative Dna Damage
Conventional Technologies for Cryopreservation of Human Oocytes and Embryos
Direct Electrical Stimulation of Myogenic Cultures for Analysis of Muscle Fiber Type Control
Single Cell Polymerase Chain Reaction for Preimplantation Genetic Diagnosis: Methods, Strategies, an
Membrane Protein NMR Studies
Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS) Method for Monitoring Methotrexate in the
Visualization of Lipid Domain-Specific Protein Sorting in Giant Unilamellar Vesicles
Microscopic Assessment of Angiogenesis in Tumors
Visualization of Adult Stem Cells Within Their Niches Using the Drosophila Germline as a Model Syste
The Direction of Ribonucleases H by Antisense Oligodeoxynucleotides
端粒顺序(telomeric sequence，TEL)
Detection of Calcium Release via Ryanodine Receptors
狗的克隆
Human Peripheral Blood Mononuclear Cell Preparation
Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration
Culture of Human Brain Tumors on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cel
c-Myb in Smooth Muscle Cells
Umbilical Cord Blood Stem Cells for Myocardial Repair and Regeneration
人工染色体(artificial chromosome)
Immobilization of Whole Cells by Chemical Vapor Deposition of Silica
Isolation and Enrichment of Stro-1 Immunoselected Mesenchymal Stem Cells from Adult Human Bone Marro
Detection of Uncoupling Protein-2 (UCP2) As a Mitochondrial Modulator of Apoptosis
Diagonal Electrophoresis for Detection of Protein Disulphide Bridges
实用哺乳动物细胞培养手册
Cell-Free Antibody Capture Method for Analysis of Detergent-Resistant Membrane Rafts
核糖核酸聚合酶（RNA polymerase）
FISH With and Without COT1 DNA
异固缩(heteropythosis)
Urine Proteins
Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Cho
Design and Analysis of Experiments
Bioinformatics Strategies for the Analysis of Lipids
Computer-Aided Sperm Analysis (CASA) of Sperm Motility and Hyperactivation
Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal Cells
Interspecific Mouse-Chick Chimeras
Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis
Screening for Epigenetic Target Genes that Enhance Reprogramming Using Lentiviral-Delivered shRNA
Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable a
ProteinProtein Interaction Techniques: Dissect PCP Signaling in Xenopus
Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Imm
Gel-Based and Gel-Free Proteomic Technologies
Nuclear Transfer in Nonhuman Primates
Nanosizing by Spatially Modulated Illumination (SMI) Microscopy and Applications to the Nucleus
Imaging Plasma Membrane Proteins by Confocal Microscopy
Homologous Gene Targeting to Study ECM Assembly
Assay for Protein Modification by Poly-ADP-Ribose In Vitro
Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Supp
Expression of EGF and HIV Envelope Glycoprotein
Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry
Extrachromosomal Assay for DNA Double-Strand Break Repair
Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Indiv
Direct Sequencing of PCR Products Using Chemiluminescent Detection
Live Imaging of LFA-1-Dependent T-Cell Motility and Stop Signals
Computer-Assisted Interpretation of Triacylglycerols Mass Spectra
胶原(collagen)
Group Migration and Signal Relay in Dictyostelium
Purifying the Hedgehog Protein and its Variants
Quantitative Visualization of Autophagy Induction by mTOR Inhibitors
Studying Early Hematopoiesis Using Avian Blastoderm Cultures
125I-UdR致C6胶质瘤细胞损伤的模型
Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector
Cationic α-Helical Peptides for Gene Delivery into Cells
Lipoplex Formation Using Liposomes Prepared by Ethanol Injection
植物油凝素的制备
Metalloproteases and the Degradome
Protocol for evaluating vascular permeability in wildtype
动物体内转染试剂
特征性研究：内源性GPCRs 细胞功能图研究
Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementation of Yeast
Preparing 48-Well Plate Feeders
LINE-1 Elements: Analysis by Fluorescence In-Situ Hybridization and Nucleotide Sequences
cos-7的转染方法讨论
Embryonic Stem Cells for Osteo-Degenerative Diseases
Development of Hematopoietic Stem and Progenitor Cells from Mouse Embryonic Stem Cells, In Vitro, Su
Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions
Cell Counting/ Live-Dead Discrimination
Sperm Selection Based on Electrostatic Charge
A Double Nuclear Transfer Technique for Cloning Pigs
Nat.Methods：新型悬浮培养技术可大规模生产干细胞
In Vivo Gene Electroporation in the Mouse Testis
Adult Ventricular Cardiomyocytes: Isolation and Culture
Analyzing DNA Replication Dynamics of Genotoxin-Treated Cells Using Velocity Sedimentation
Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes
Purification of MMPs and TIMPs
Detecting sRNAs by Northern Blotting
Ca2+ Imaging as a Tool to Assess TRP Channel Function in Murine Distal Nephrons
Regulation of Convergent Extension by Non-canonical Wnt Signaling in the Xenopus Embryo
Use of Codon Cassette Mutagenesis for Saturation Mutagenesis
Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos: Optimal Ring and Rol
Mitochondrial DNA Mutations: An Overview of Clinical and Molecular Aspects
Analysis and Classification of Serum Cryoglobulins
Studying Proteolysis Within Mitochondria
Progenitor Cell Mobilization from Extramedullary Organs
Electron Microscopic Imaging of Integrin
Determination of Carbonyl Groups in Oxidized Proteins
Equilibrium Binding and Transport Studies
Reprogramming of Committed Lymphoid Cells by Enforced Transcription Factor Expression
Generation of Chondrocytes From Embryonic Stem Cells
Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo
结晶紫染色法测定细胞数
In Silico Identification of Novel G Protein Coupled Receptors
Doxycycline Regulated Lentiviral Vectors
Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos
Large Animal Model of Heart Failure for Assessment of Stem Cells
嵴(cristae)
Angiogenesis Assays in the Chick CAM
Pharmacogenetics: Defining the Genetic Basis of Drug Action and Inositol Trisphosphate Analysis
Analysis of RNA Structure and RNA-Protein Interactions in Mammalian Cells by Use of Terminal Transfe
Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow
Human Myometrial Smooth Muscle Cells and Cervical Fibroblasts in Culture: A Comparative Study
Analysis of Tubulin Transport in Nerve Processes
RNAi in the Hedgehog Signaling Pathway: pFRiPE, a Vector for Temporally and Spatially Controlled RNA
Purification and Identification of Protein Complexes That Control the Cell Cycle
In Ovo Gene Electroporation into Early Chicken Embryos
Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters
Identification, Characterization, and Differentiation of Human Prostate Cells
Biochemical Methods to Determine Cell-Surface Topography: Part A. Labeling of Oxidized Glycoproteins
Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes
Generation of Mouse Embryos with Small Hairpin RNA-Mediated Knockdown of Gene Expression
Potential Zoonotic Infection of Porcine Endogenous Retrovirus in Xenotransplantation
Microsomal Proteomics
Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation
Simultaneous Immunofluorescence Detection of Cadherin and -Catenin in Mouse Tissue Sections
Hepatic Maturation of hES Cells by Using a Murine Mesenchymal Cell Line Derived from Fetal Livers
人胰岛β细胞酶联免疫分析（ELISA）
Large-Scale Production and Storage of Monoclonal Antibodies and Hybridomas
Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
Using Immuno-Scanning Electron Microscopy for the Observation of Focal Adhesion-substratum interacti
Imaging Cell Movement During Dorsal Closure in Drosophila Embryos
Optical Control of Ligand-Gated Ion Channels
C-Terminal Sequence Analysis of 2DE-Separated Proteins
Amino Acid Regulation of Autophagosome Formation
Cell-Surface ProteinProtein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies:
Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro
γ微管蛋白(γ tubulin)
In Vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation
Activation and Function of Small GTPases Rho, Rac, and Cdc42 During Gastrulation
Heart Xenotransplantation in Primate Models
cDNA Subtraction and Cloning in the Field of Trophoblast/Placental Development
Treatment of a Tumor Model with ECT Using 4+4 Electrode Configuration
配子发生(gametogenesis)
细胞转染后缩为小圆点
Analysis of MMP-Dependent Cell Migration and Invasion
Random Mutagenesis by Using Mixtures of dNTP and dITP in PCR
Quantitative In Situ Analysis of Claudin Expression at the BloodRetinal Barrier
Synchronization of HeLa Cells
Determination of Aliphatic Low-Molecular-Weight and Biogenic Amines by Capillary Zone Electrophoresi
Isolation of Satellite Cells from Single Muscle Fibers from Young, Aged, or Dystrophic Muscles
Silver Sequencing: Nonradioactive Cycle Sequencing of dsDNA
Methodology, Biology and Clinical Applications of Human Mesenchymal Stem Cells
DNA Fragmentation Assays for Apoptosis Protocol
Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-Dimensional Collagen Matrix
Embryonic Stem Cell Differentiation and the Vascular Lineage
Evaluation of Cell Morphology
Use of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent Blood Coagulat
Insulin-Producing Cells from Embryonic Stem Cells Experimental Considerations
Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation
Reconstitution of the Nuclear Transport of the MAP Kinase ERK2
Use of Firefly Luciferase Activity Assays to Monitor Circadian Molecular Rhythms In Vivo and In Vitr
Investigation of KATP Channel Endocytosis by Immunofluorescence
Short-Term Chorionic Villi and Amniotic Fluid Cultures
Internalization of Nucleoside Phosphates into Live Cells by Complex Formation with Different CPPs an
Monitoring Sterol Uptake, Acetylation, and Export in Yeast
Elastic Liposomes for Topical and Transdermal Drug Delivery
New Opportunities for Immobilization of Enzymes
Flow Cytometry Analysis of Cell Cycling and Proliferation in Mouse Hematopoietic Stem and Progenitor
Immunofluorescence Analysis of Circadian Protein Dynamics in Cultured Mammalian Cells
Design of Experiments to Investigate Dynamic Cell Signaling Models
Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodies
CD8+, CD8, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand
Bone Marrow-Derived Mesenchymal Stem Cells: Isolation, Expansion, Characterization, Viral Transducti
MTT方法的使用步骤
糖原的显示―PAS反应（periodic acid schiff’s reaction）
Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC)
Using 2D Crystals to Analyze the Structure of Membrane Proteins
细胞培养基中常用维生素及其作用
Cloning of Potassium Channel Splice Variants from Tissues and Cells
Immunohistochemistry of Xenopus Embryos
Isolation of Hair Follicle Bulge Stem Cells from YFP-Expressing Reporter Mice
Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts
Functional Assays for Hematopoietic Stem Cell Self-Renewal
Biomarkers of Cell Senescence Assessed by Imaging Cytometry
PMEF FEEDER LAYER CONCENTRATION
Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue
ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/M
The Intranuclear Environment
Culture Systems: Mineral Oil Overlay
Studying Mitochondria in an Attractive Model: Schizosaccharomyces pombe
An Integrated Approach for Monitoring Cell Senescence
Analysis of Cell Generation in theTelencephalic Neuroepithelium
染色体扫描电镜标本制备与观察
Characterization of Calcium/Calmodulin- Stimulated Protein Kinase II
Measurement of Calcium Release Due to Inositol Trisphosphate Receptors in Skeletal Muscle
Adipogenic Differentiation of Human Mesenchymal Stem Cells
Lentiviral Vectors to Study the Differential Function of ERK1 and ERK2 MAP Kinases
Methods for Constructing and Producing Retroviral Vectors
Human Peripheral Blood Mononuclear Cell Preparation
Culture and Differentiation of Mouse Tracheal Epithelial Cells
A High Content Imaging-Based Approach for Classifying Cellular Phenotypes
人酸性成纤维细胞生长因子（aFGF）酶联免疫分析（ELISA）
细胞骨架系统(cytoskeletonic system)
Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresi
细胞分化(cell differentiation)
Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative G
Using Endogenous MicroRNA Expression Patterns to Visualize Neural Differentiation of Human Pluripote
Human Vascular Smooth Muscle Cell Culture
Identification of Differentially Expressed Fungal Genes In Planta by Suppression Subtraction Hybridi
Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death
High-Throughput Screening of Mutant -Amylase Libraries for Increased Activity at 129C
Characterization of Cellular Internalization Pathways for CPP-Mediated Oligonucleotide Delivery
In Vitro Study of Nuclear Assembly and Nuclear Import Using Xenopus Egg Extracts
推荐：细胞冻存步骤的专业实验流程
Spermatogonial Stem Cells
Crystal Violet Assay
Three-Dimensional Cultures of Mouse Mammary Epithelial Cells
Microtubule Spindowns for Visual Analysis
稳定转染中的问题:从G418浓度确定到单克隆化鉴定
转染的时候能否用加血清的培养基
Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis
主动运输
实验室各种板子的用法比较
Mimicry Technology: Suppressing Small RNA Activity in Plants
核膜
细胞凋亡有什么特点？
ARS 序列(autonomous replicating sequence)
Novagen公司推出无需IPTG的自动诱导大肠杆菌培养基
In Vitro Stimulation and Detection of IFN Production in Human Plasmacytoid Dendritic Cells
Determination of Oxidized and Reduced CoQ10 and CoQ9 in Human Plasma/Serum Using HPLC-ECD
Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions
Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Str
Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Mem
Endogenous Inhibitory Mechanisms and the Regulation of Platelet Function
关于细胞培养基的几点讨论
Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques
Resources for Genetic and Genomic Studies of Xenopus
The Isolation of Nuclei and Nuclear Membranes from Rat Liver
Functional Analysis of Cell Adhesion Molecules
Liposomal siRNA Delivery
Single-Molecule Methods to Study Cell Adhesion Molecules
Detection and Measurement of Necrosis in Plants
Fluorescence Methods in the Study of Small GTP-Binding Proteins
Antisense Strategies for Treatment of Heart Failure
Gene Expression and Methylation Patterns in Cloned Embryos
Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine
Cell Sorting By FACS
Identifying Myc Interactors
Flow Cytometric Analysis of Drosophila Cells
Measurement of Ion Fluxes and pH Gradients Across Cell Membranes
猪白细胞介素-8（IL-8）酶联免疫分析（ELISA）
山羊脑炎（encephalitis）酶联免疫分析（ELISA）
Applications of Cell-Penetrating Peptides as Signal Transduction Modulators for the Selective Induct
Antisense Therapy of Hepatitis B Virus Infection: In Vivo Analyses in the Duck Hepatitis B Virus Mod
Optimizing Electroporation Conditions for Intracellular Delivery of Morpholino Antisense Oligonucleo
Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis
Assessing Organismal Aging in the Filamentous Fungus Podospora anserina
End-Labeling and Analysis of Spo11-Oligonucleotide Complexes in Saccharomyces cerevisiae
Use of Differentiating Embryonic Stem Cells in the Parkinsonian Mouse Model
Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Biolu
Screening for -Ray Hypersensitive Mutants of Arabidopsis
Cellulose Paper Support for Immobilization
The Dual Use of RNA Aptamer Sequences for Affinity Purification and Localization Studies of RNAs and
Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora
流式细胞术分析血小板
Neural Cell Lines
Quantitative Studies of Neuronal Chemotaxis in 3D
The IUBMB-Endorsed Transporter Classification System
Uses of Telomerase Peptides in Anti-Tumor Immune Therapy
培养293细胞应注意的几个问题
Markers of Cellular Senescence
出胞与入胞
融合蛋白(fusion protein)
Measurement of Filter Paper Bloodspot Lead by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)
A convenient method for the isolation of crude nuclear pellets.
联合作图（association mapping）和单倍体型（haplotype）
扫描隧道显微镜(scanning tunneling microscope，STM)
Wnt-Frizzled Interactions in Xenopus
Spermatogenesis in Cryptorchidism
Analysis of Two Aspects of Left-Right Patterning of the Vertebrate Heart: Heart Tube Position and He
Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Livi
Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts
Hematopoietic Progenitor Cell Apheresis Processing
动物细胞大规模培养用生物反应器的简介与分类
Probing mRNA Structure and sRNAmRNA Interactions in Bacteria Using Enzymes and Lead(II)
Continuous Infusion of Antisense Phosphorothioate Therapeutics
The Measurement of Phospholipase D- Linked Signaling in Cells
Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Cand
Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells
Robust Multiparametric Assessment of Cellular Senescence
Measurement of Cyclic GMP Formation
Directed Differentiation of Pluripotent Stem Cells to Functional Hepatocytes
Steroid Analysis by Micellar Electrokinetic Capillary Chromatography
Analysis of the C. elegans Germline Stem Cell Region
Selection of Peptides Interfering with ProteinProtein Interaction
Localized Sampling, Electrophoresis, and Biosensor Analysis of Xenopus laevis Cytoplasm for Subcellu
Double-Stranded DNA Site-Directed Mutagenesis
History of Antisense Oligonucleotides
Transformation of Fish Cells and Embryos
In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast
细胞调亡的研究方法：琼脂糖凝胶电泳
Plasmid Replicon Typing
Isolation and Manipulation of Mammalian Neural Stem Cells In Vitro
Micro-structured Materials and Mechanical Cues in 3D Collagen Gels
Functional Expression of CXCR4 in Saccharomyces cerevisiae in the Development of Powerful Tools for
Interleukin-27 Induces Interferon-Inducible Genes: Analysis of Gene Expression Profiles Using Affyme
Detection and Activation of Stress-Responsive Tyrosine Kinases
ProteinProtein Interactions in Signaling Cascades
细胞培养基的基本要求、组成及作用
Measurement of Apoptosis by DNA Fragmentation
The Use of PARP Inhibitors in Cancer Therapy: Use as Adjuvant with Chemotherapy or Radiotherapy; Use
L-selectin-PNAd Interactions under Flow Conditions.
Cell Clonogenic Survival Assay
细胞培养中常见的污染情况
细胞培养的一般技术
Flow Cytometric Analysis Of Bcl Family members
电转感受态菌TG1的制备
Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approac
Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts a
干细胞的冻存方法与细胞计数
新生牛血清的筛选
荧光激活的细胞分类仪检测调亡细胞
什么是细胞凋亡？
关于小鼠腹腔巨噬细胞的几个问题
Protocol for intracytoplasmic staining of cytokines for FACS analysis
人可溶性白细胞分化抗原40配体（sCD40L）酶联免疫分析
人源TCTP可调控mTOR信号通路
Tube formation assay
红细胞比容（hematocrit value）
Growing feeder-independent embryonic stem cells§
Measuring mtDNA Damage Using a Supercoiling-Sensitive qPCR Approach
Tumor Cell Invasion Assays
A Novel Method for the Production of Fully Modified K-Ras 4B
Genome-Wide Overexpression Screen for Activators of Antioxidant Gene Transcription
Chemotaxis Assay
酸性磷酸酶法检验细胞增殖
Retroviral Gene Transduction in Limb Bud Micromass Cultures
Retroviral Integration Site Analysis in Hematopoietic Stem Cells
Resonance Raman Spectroscopy of Metalloproteins Using CW Laser Excitation
Canine Models of Gene-Modified Hematopoiesis
Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of RNA
Isolation and Culture of Mouse Intestinal Cells
Examination of Normal and Abnormal Placentation in the Mouse
流式细胞仪主要技术指标
Transfection and Transformation of Human Thyroid Epithelial Cells
细胞粘着(cell adhesion)
$冰冻断裂(freeze fracture)$
冰冻断裂(freeze fracture)
Coculture of Decidua and Trophoblast to Study Proliferation and Invasion
Preparation and Analysis of Uniquely Positioned Mononucleosomes
Screening of Telomerase Inhibitors
Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Gerany
核仁周期(nucleolar cycle)
The Introduction of Proteins into Mammalian Cells by Electroporation
PCR Sequencing with the Aid of Detergents
Real-Time Imaging of Meiotic Chromosomes in Saccharomyces cerevisiae
Methods for Culturing Human Embryonic Stem Cells on Feeders
科学委员会支持南非建最大射电望远镜
Hematopoietic Development of ES Cells in Culture
Nuclear Transplantation in Xenopus
Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization
Imaging Actin Cytoskeleton Dynamics in Dictyostelium Chemotaxis
Analysis of B-Cell Life-Span and Homeostasis
Detergent-Extracted Models for the Study of Cilia or Flagella
The Voltage-Clamp Fluorometry Technique
Remote Loading of Anthracyclines into Liposomes
Cell Lineage Analysis: Applications of Green Fluorescent Protein
Microtubule Binding Assays
Detection of Nuclear Envelope Alterations in Senescence
A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction
母体信息（maternal information）
In situ Hybridization with RNA Probes
Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological condi
Immunostaining of Skeletal Tissues
Analysis of tRNA-Directed Transcription Antitermination in the T Box System In Vivo
Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study
Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies
Histopathologic Assessment of Myocardial Regeneration
Mitochondrial Respiratory Chain: Biochemical Analysis and Criterion for Deficiency in Diagnosis
An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations
Methods for Analysis of Crossover Interference in Saccharomyces cerevisiae
Generation of a Vascular Niche for Studying Stem Cell Homeostasis
Cell Kinetic Analysis in Artificial Skin Using Immunochemical Methods
Effective In Vitro and In Vivo Gene Delivery by the Combination of Liposomal Bubbles (Bubble Liposom
T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences
Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display
A Systems Approach Demonstrating Sphingolipid-Dependent Transcription in Stress Responses
Detection and Quantification of Cytokines and Other Biomarkers
Preparation of GTPases for Structural and Biophysical Analysis
Quantification of Human Cytomegalovirus by Competitive PCR and Capillary Electrophoresis
Detection of Human and Mouse Granzyme B Activity in Cell Extracts
Cloning PCR Products for Sequencing in M13 Vectors
Isolation of Neuroepithelium and Formation of Minispheres
Culture of Human Renal Medullary Interstitial Cells
Atomic Force Microscopy for Analyzing Metaphase Chromosomes: Comparison of AFM Images with Fluoresce
Designing and Utilization of siRNAs Targeting RNA Binding Proteins
Fluorescent Transgenes to Study Interphase Chromosomes in Living Plants
Immunofluorescence Methods in Studies of the GTPase Ran and Its Effectors in Interphase and in Mitot
Cryopreservation of the Sperm of the Pacific Oyster Crassostrea gigas
Basic Analyses of Proteins of Interest for Xenotransplantation
Wingless Signaling in Drosophila Eye Development
A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene
Protein Separation Using Immobilized Phospholipid Chromatography
Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism
Epiggenetics in Dictyostelium
Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells
In Vitro Maturation and In Vitro Fertilization of Human Oocytes
Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation
Frizzled-7-dependent Tissue Separation in the Xenopus Gastrula
Construction of Linker-Scanning Mutations Using PCR
Localization and MHC Class II Presentation of Antigens Targeted for Macroautophagy
Navigation to the Graveyard-Induction of Various Pathways of Necrosis and Their Classification by Fl
Isotope Labeling Strategies for Analysis of an Ion Channel Cytoplasmic Domain by NMR Spectroscopy
Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids
Dissecting Nucleases into Their Structural and Functional Domains: Mapping the RNA-Binding Surface o
TEM Visualization of Microtubules
Zymography and Reverse Zymography for Detecting MMPs, andTIMPs
Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts
Studying Vascular Progenitor Cells in a Neonatal Mouse Model
Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Mu
Synthesis of Polyampholyte Comb-Type Copolymers Consisting of a Poly(L-lysine) Backbone and Hyaluron
Isolation of Rat Intestinal Lymph DC
Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
Studying the Mechanosensitivity of Voltage-Gated Channels Using Oocyte Patches
Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separ
Adipose-Derived Stem Cells for Periodontal Tissue Regeneration
Measurements of Glucose Conversion to its Metabolites
Stabilization of Polycation-DNA Complexes by Surface Modification with Hydrophilic Polymers
Isolation, Culture, and Characterization of Human Umbilical Cord Stroma-derived Mesenchymal Stem Cel
Characterization of STAT Self-Association by Analytical Ultracentrifugation
DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73)
An Easy and Reliable Automated Method to Estimate Oxidative Stress in the Clinical Setting
In Vitro and In Vivo Protein Import Into Plant Mitochondria
Generating stable cell lines in HEK293
Cell Cycles - Introduction
Protein Techniques: Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting
293细胞的大规模培养三种方式
Isolation and Characterization of Distal Lung Progenitor Cells
类囊体(thylakoid)
Electron Microscope Visualization of RNA Transcription and Processing in Saccharomyces cerevisiae by
An In Vitro Assay for the Kinase Activity of mTOR Complex 2
Protein-Thiol Oxidation, From Single Proteins to Proteome-Wide Analyses
Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems
Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Gr
Treatment of Multiple Spontaneous Breast Tumors in Mice Using Electrochemotherapy
Isolation and Culture of Rabbit Embryonic Stem Cells
Transgenic Systems in Nuclear Reprogramming
Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High-Densit
Genetic Manipulation of Keratinocyte Stem Cells with Lentiviral Vectors
Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aort
Studying Nuclear Reprogramming with Cell Hybrids
The Identification of Subcellular Fractions from Mammalian Cells
Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
Methods for Studying Checkpoint Kinases Chk1
Prespliceosome and Spliceosome Isolation and Analysis
Long-Term B-Lymphoid Cultures from Murine Bone Marrow Establishment and Cloning by Using Stromal Cel
Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software
GeneCalling: Transcript Profiling Coupled to a Gene Database Query
Deep Sequencing of Small Chromatin-Associated RNA: Bioinformatic Analysis
Recognition Imaging of Chromatin and Chromatin-Remodeling Complexes in the Atomic Force Microscope
Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines
The Calvarial Injection Assay
Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Cult
愈伤组织(callus， culli)
Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes
Generation of Human Melanocytes from Induced Pluripotent Stem Cells
Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells
Feeder-Layer Free Culture System for Human Embryonic Stem Cells
A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered
Media Composition: Salts and Osmolality
Bioreactor Cultivation of Functional Bone Grafts
Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment L
Trophoblast Differentiation: An In Vitro Model for Trophoblast Giant Cell Development
Direct Sequencing of PCR Products with DNA-Binding Proteins
Visualization of mRNA Expression in the Zebrafish Embryo
Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines
Fourier Transform Infrared Imaging of Bone
Transient Overexpression of Genes in Neurons Using Nucleofection
Isolation and Culture of Mouse Satellite Cells
Differential mRNA Display: Adaption for In Vivo Studies of Diseased Tissues
Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes
Quantification of Cyclobutane Pyrimidine Dimers in Human Epidermal Stem Cells
HPLC/MS/MS-Based Approaches for Detection and Quantification of Eicosanoids
初级溶酶体(primary lysosome)
Assays of Adrenergic Receptors: Including Lipolysis and Binding Measurements
Role of Endothelial CellCell Junctions in Endothelial Permeability
Purification of Plant Ribosomal DNA: Use of Buoyant Density Centrifugation in Cesium Chloride-Actino
Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports
Visualization and Interpretation of Eukaryotic DNA Replication Intermediates In Vivo by Electron Mic
Rat Brain Neuropeptidomics: Tissue Collection, Protease Inhibition, Neuropeptide Extraction, and Mas
Generation of Tissue Organoids by Compaction Reaggregation
Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Ma
Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs)
Murine Bone Marrow-Derived Macrophages
纺锤体（spindle）
Sos蛋白(Sos protein)
Melanosome Motility in Fish Retinal Pigment Epithelial Cells
Visualization of Induced RNA in Single Bacterial Cells
Isolation and Short-Term Culture of Human Epidermal Langerhans Cells
Visualization of Spindle Behavior Using Confocal Microscopy
Yeast Protoplast Fusion
Immobilization of Enzymes and Proteins on Red Blood Cells
The Measurement of Protein Degradation in Response to Oxidative Stress
Cell Viability Assays: Introduction
In Situ Hybridization with Nonradioactive Probes
Analysis of Fungal Gene Expression by Real Time Quantitative PCR
Detection of Protein Glutathionylation
导肽(leading peptide)
心肌细胞培养方法详述
Identification of Genes Involved in Tumor-Like Properties of Embryonic Stem Cells
Analysis of Chromatin Composition of Repetitive Sequences: The ChIP-Chop Assay
Review of Molecular Mechanisms Involved in the Activation of the Nrf2-ARE Signaling Pathway by Chemo
Analysis of Fatty Acids by Gas Liquid Chromatography
Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy
In Vivo Imaging of Tight Junctions Using ClaudinEGFP Transgenic Medaka
Culturing Chick Embryonic Cardiac Myocytes and Fibroblasts
Visualization of PCP Defects in the Eye and Wing of Drosophila melanogaster
Cdc28蛋白(Cdc 28 protein)
Acute Behavioral Responses to Pheromones in C. elegans (Adult Behaviors: Attraction, Repulsion)
Measurement of Caspase Activity: From Cell Populations to Individual Cells
Skeletogenesis: In Vitro Analysis of Bone Cell Differentiation
同源异型框(homeobox)
Use of the Cytomegalovirus Promoter for Transient and Stable Transgene Expression in Mouse Embryonic
Manipulation of Gene Function in Xenopus laevis
Comprehensive mRNA Profiling of Lipid-Related Genes in Microglia and Macrophages Using Taqman Arrays
In a magnetofection procedure, self-assembling complexes of enhancers like cationic lipids with plas
Liposomal Magnetofection
Semi-quantitative Analysis of Transient Single-Cell Gene Expression in Embryonic Stem Cells by Femto
Directed Alteration of Saccharomyces cerevisiae Mitochondrial DNA by Biolistic Transformation and Ho
Primary Culture and Propagation of Human Prostate Epithelial Cells
Overview of Integrin Signaling in the Immune System
Analysis of Cell Shape and Polarity During Zebrafish Gastrulation
Staging of Mouse Seminiferous Tubule Cross-Sections
Genetically Encoded Probes Provide a Window on Embryonic Arrhythmia
The Identification of Signaling Molecules by the Yeast Two-Hybrid System
Assays for Chemotaxis and Chemoattractant-Stimulated TorC2 Activation and PKB Substrate Phosphorylat
Generation and Characterization of Murine Alternatively Activated Macrophages
Primary Mouse Small Intestinal Epithelial Cell Cultures
Isolation of Neural Stem and Precursor Cells from Rodent Tissue
Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium
再生(regeneration)
Adult Neural Stem Cells
Detection and Measurement of Membrane-Bound Protein Tyrosine Kinases in the Zebrafish Egg
Establishing Relationships Between Closely Related Species Using Total Genomic DNA as a Probe
Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp T
Mutagenesis in Arabidopsis
剪接体(spliceosome)
Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression
Working Cell Bank
Covalent Immobilization of Enzymes to Graphitic Particles
Manipulating and Imaging the Early Xenopus laevis Embryo
Derivation and Culture of Canine Embryonic Stem Cells
Direct Sequencing of Differential Display PCR Products
Two-Hybrid Analysis of RasRaf Interactions
Special Stains for Extracellular Matrix
Direct MALDI-TOF Mass Spectrometric Peptide Profiling of Neuroendocrine Tissue of Drosophila
Manipulation of Gene Expression During Zebrafish Embryonic Development Using Transient Approaches
Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells
Restriction Enzymes
Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins
Techniques for the Study of Apoptosis in Bone
Measurement of Low-Frequency DNA Breaks Using Nucleoid Flow Cytometry
Assessing the Involvement of Telomerase in Stem Cell Biology
Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration
Identification and Characterization of Small GTPase-Associated Kinases
Establishing Cell-Based Reporter Systems for the Analysis of hTERT Expression
Raf/MEK/MAPK Signaling Stimulates the Nuclear Translocation and Transactivating Activity of FOXM1
Immobilization of Enzymes on Inorganic Supports by Covalent Methods
Differentiating Embryonic Stem Cells into Embryoid Bodies
Techniques in Protein Methylation
Xenopus tropicalis Oocytes: More Than Just a Beautiful Genome
Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in th
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
磷脂(phospholipids)
Eukaryote Growth Dynamics
植物遗传转化中基因受体的选择
吞噬作用(phagocytosis)
Isolation and Culture of Rhesus Adipose-Derived Stem Cells
Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias latipes
A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptor
肌动蛋纤维去聚合蛋白(actin filament-depolymerizing protein)
Treatment of Tissue Sections for In Situ Hybridization
Imaging Drosophila Pupal Wing Morphogenesis
Analyses of Gene Function in Amphioxus Embryos by Microinjection of mRNAs and Morpholino Oligonucleo
Lipidomics of the Red Cell in Diagnosis of Human Disorders
Nucleocytoplasmic Glycosylation, O-GlcNAc: Identification and Site Mapping
Protocols for Nuclear Transfer in Mice
Hypoxia-Inducible Factors: Post-translational Crosstalk of Signaling Pathways
Female Moth Pheromones
Studying Recombination in Mouse Oocytes
Genetic Mapping of Anti-Apoptosis Pathways in Myeloid Progenitor Cells
Isolation of Actin and Myosin Filaments
Study of Cell Cycle Checkpoints Using Xenopus Cell-Free Extracts
In Vivo Skin-Targeted Gene Delivery by Pulsed Electric Fields
Constitutively Active STAT5 Constructs
Efficient Generation of Dopamine Neurons from Human Embryonic Stem Cells
Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs
Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by
Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Resp
Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations
A High-Throughput Mammalian Cell-Based Transient Transfection Assay
锚定蛋白(ankyrin)
Stepwise Differentiation from Nave State Pluripotent Stem Cells to Functional Primordial Germ Cells
An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells
Vertical Arrays: Microarrays of Complex Mixtures of Nucleic Acids
Fusion Protocol for the Production of Mouse Hybridomas
Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells
An Assay for Human Chemosignals
Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells
Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells
质体(plastid)
Cdc34 蛋白（Cdc34 protein）
Analysis of Mammary Gland Morphogenesis
Immunohistochemical Analysis of mTOR Activity in Tissues
The Animal Cap Assay
Genetic Manipulation of Human Embryonic Stem Cells by Transfection
Micro-CT Technique for Three-Dimensional Visualization of Human Stem Cells
Detection of Gene Expression in Mouse Embryos and Tissue Sections
The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids
Immunostaining of Germline Stem Cells and the Niche in Drosophila Ovaries
Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection
美国JRH公司EX-CELLTM 302无血清培养基配制方法
In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections
Stabilization of Immobilized Enzymes by Chemical Modification with Polyfunctional Macromolecules
Experimental Manipulation and Morphometric Analysis of Neural Tube Development
Quail-Chick Chimeras
酵母遗传学方法2：蛋白质的抽提
数量性状实验
转运蛋白(translocator)
Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Re
Measurement of Phospholipase C Activity in Brain Membranes
PET-Radioimmunodetection of Integrins: Imaging Acute Colitis Using a 64Cu-Labeled Anti-7 Integrin An
阻断FC受体方法
Analyzing CellCell Interactions in 3-Dimensional Adhesion Assays
Human Mesenchymal Stem Cell Culture for Neural Transplantation
局部介质(local mediators)
Mammalian cells are amenable to study the regulation of cell cycle progression in vitro by shifting
Synchronization of Mammalian Cell Cultures by Serum Deprivation
Histological Approaches to Study Tissue Parasitism During the Experimental Trypanosoma cruzi Infecti
Cell-free System for the examination of apoptotic activity in cytoplasmic extracts
Analysis of DNA Topology in Yeast Chromatin
Quantitation of 25-OH-Vitamin D (25OHD) Using Liquid Tandem Mass Spectrometry (LC-MS-MS)
Human Prostate Epithelial Cell Cultures
Monitoring Removal of Cyclobutane Pyrimidine Dimers in Arabidopsis
Flow Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products
Detection of Plasmid by Colony Hybridization
In Situ Hybridization with Radiolabeled cRNA Probes, Using Tissue Sections and Smears
Assessment of Embryotoxicity Using Mouse Embryo Culture
Analysis of CTG Repeats Using DM1 Model Mice
Mitochondrial genome integrity is an important issue in somatic mitochondrial genetics. Development
Collection of Isolated Cells for Studying Mitochondrial DNA Mutations Within Individual Cells
通讯连接(communicating junction)
Motility Signaled From the EGF Receptor and Related Systems
Transgenic Reporter Tools Tracing Endogenous Canonical Wnt Signaling in Xenopus
DNA Ligation and Escherichia coli Transformation
支原体污染的特点及检验的笔记
原代细胞冻存实验操作步骤
Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Re
Production of Avian Chimeras and Germline Transmission
Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System
Microinjection of Antisense Oligonucleotides and Electrophysiological Recording of Whole-Cell Curren
Isolation and Grafting of Single Muscle Fibres
Isolation and Cultivation of Dermal Stem Cells that Differentiate into Functional Epidermal Melanocy
Generation of Multipotent CD34+CD45+ Hematopoietic Progenitors from Human Induced Pluripotent Stem C
Embryonic Stem Cell Derivation from Human Embryos
Cytogenetic Techniques for Human Leukemias
Peptide Recognition Mechanisms of Eukaryotic Signaling Modules
Rat x Rat Hybridomas
Generation of iPS Cells from Human Skin Biopsy
Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movem
Using Small Molecules to Improve Generation of Induced Pluripotent Stem Cells from Somatic Cells
Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration
中国首次研制出流感嗜血杆菌选择性培养基
生物安全柜与通风柜、超净工作台的区别
细胞电融合实验
细胞培养最常见注意事项
Nuclear Gene Defects in Mitochondrial Disorders
Fluorescence-Activated Cell Sorting According to Receptor Density: Application for Isolating Transfe
Xenotransplantation of Embryonic Stem Cell-Derived Motor Neurons into the Developing Chick Spinal Co
Retroviral Delivery of ECM Genes to Cells
Identification of Transplanted Human Cells in Animal Tissues
Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins
Assessing Tumor Angiogenesis in Histological Samples
偶线期(zygotene stage)
Use of Liposomes to Study Vesicular Transport
Claudin-5 Expression in In Vitro Models of the BloodBrain Barrier
Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Developme
Isolation and Characterization of Stem Cell-Enriched Human and Canine Hair Follicle Keratinocytes
Methods to Induce Cell Cycle Checkpoints
Monoclonal Antibodies in the Analysis of Embryonic Development
质体醌(plastoquinone, PQ)
ES Cell Culture and Manipulation
单克隆抗体细胞融合实验操作步骤及注意事项
Adsorbed Cationic Polymer Coatings for Enhanced Capillary Electrophoresis/Mass Spectrometry of Prote
SAPK/Jun kinase assays
联会复合体（synaptonemal complex,SC）
Targeted Mutagenesis Mediated by the Triple Helix Formation
Molecular Profiling of Hematopoietic Stem Cells
Determination of Functional Viral Titer by Drug-Resistance Colony Assay, Expression of Green Fluores
原核细胞(prokaryotic cell)
大鼠肌钙蛋白T(Tn-T)酶联免疫分析（ELISA）
细胞分裂的形态观察
Method: Lymphocyte Transformation
T-Cell Activation Using mAb to CD3
Gain-of-Function and Loss-of-Function Strategies in Xenopus
Arabidopsis Mitochondrial Proteomics
原代神经细胞培养实验方法
Linear RNA Amplification for the Production of Microarray Hybridization Probes
Abnbio dpbs缓冲液 A141905 不含氯化钙、氯化镁和酚红 500ml
Abnbio DMEM高糖培养基,High Glucose（无丙酮酸钠） A119655 500ml
Abnbio M199培养基 EBSS*含L-谷氨酰胺 L-氨基酸 A111505 500ml
Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration, and Tumorigenesis
Abnbio MEM培养基含L- 谷氨酰胺 A110955 500ml
Protein Extraction From Mammalian Tissues
Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors
Oxidative Stress and Plant Mitochondria
植物组织细胞培养
细胞生长检测9：染料染色法测定细胞数
Mass Spectrometry Analysis of Polyisoprenoids Alcohols and Carotenoids via ESI(Li+)-MS/MS
Preparation of Rat Liver Cell Cytosol
Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation
Determination of Tetrahydrozoline in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-
原生质理论（protoplasm theory）
Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protoco
Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in
Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differentiation Factors
Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell Plates
Isolation and Purification of Functionally Intact Mitochondria from Plant Cells
S期促进因子(S phase-promoting factor,SPF)
PEG介导的动物细胞融合技术
细胞分泌(cell secretion)
Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics
Dorsal Axis Duplication as a Functional Readout for Wnt Activity
High-Throughput FACS Method for Directed Evolution of Substrate Specificity
Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemi
Fate of Desmosomal Proteins in Apoptotic Epidermal Cells
ISH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results
Detection of Ubiquitination in 2DE
Redox-Sensitive Green Fluorescent Protein: Probes for Dynamic Intracellular Redox Responses. A Revie
Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection
In Vivo SELEX in Vertebrate Cells
Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedur
In Vitro Maturation (IVM) of Porcine Oocytes
Nuclear Transfer in Cattle
Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins
推荐一种简单实用的细胞冻存方法
Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes
In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tub
Practical Approaches for Implementing Forward Genetic Strategies in Zebrafish
Detection and Measurement of Reactive Oxygen Intermediates in Mitochondria and Cells
Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells
Strategies to Isolate Evolutionarily Conserved Cell Cycle Regulatory Genes
Restriction Site Mapping
人可溶性细胞间粘附分子1(sICAM-1)酶联免疫分析（ELISA）
膜结合核糖体(membrane-bound ribosomes)
Studying Osteocyte Function Using the Cell Lines MLO-Y4 and MLO-A5
动物细胞融合实验
Detection of Apoptosis in Tissue Sections
Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using th
Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy
Adipose-Derived Stem Cells for Skin Regeneration
Tat-Mediated Delivery of Antibodies into Cultured Cells
High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout
Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells
Site-Directed Mutagenesis Using a Rapid PCR-Based Method
Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary
Tips and Tricks for mFISH
Purification and Characterization of Poly(ADP-Ribosyl)ated DNA Replication/Repair Complexes
FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast
High-Resolution NMR of DNA and Drug-DNA Interactions
Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells
Percent Labeled Mitosis Curve Analysis
Electroporation-Mediated Gene Transfer into Hepatocytes
Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids
细胞生物基本方法：神经组织细胞培养
Antisense-Mediated Inhibition of Protein Synthesis: Rational Drug Design, Pharmacokinetics, Intracer
Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium
Protein Extraction for 2DE
Freezer and Cell Line Database
Assay of Phospholipase D Activity in Cell-Free Systems
Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of Mesenchymal Chondrogenesis
Flow Cytometry and Immunoselection of Human Stem Cells
Reprogramming of B Cells
Harvesting Hematopoietic Cells from Mice
Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model
Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cel
Staining Methods for cell death
培养基制备的基础实验
Generation of a Pool of Human Platelet Lysate and Efficient Use in Cell Culture
N-连接糖基化(N-linked glycosylation)
单链断裂重组模型（model of single-strand breaks recombination）
Gel Electrophoresis-Based Proteomics of Senescent Tissues
N-连接糖基化(N-linked glycosylation)
单链断裂重组模型（model of single-strand breaks recombination）
Gel Electrophoresis-Based Proteomics of Senescent Tissues
RAP-PCR Using RNA from Tissue Microdissection
Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1)
Measurement of Activities of Cyclobutane-Pyrimidine-Dimer and (6-4)-Photoproduct Photolyases
体外细胞培养对细胞培养基几点要求
EB病毒诱导淋巴细胞永生化实验方法
Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for th
Ultrasound-Responsive Liposomes
Slug Phototaxis, Thermotaxis, and Spontaneous Turning Behavior
In Situ Localization of Apoptosis Using TUNEL
Quantitative Analysis of miRNA Expression in Epithelial Cells and Tissues
Cell-Free Fetal DNA Plasma Extraction and Real-Time Polymerase Chain Reaction Quantification
Clinical Trials for Solid Tumors Using Electrochemotherapy
Neurotransmitter Receptor Analysis in Transgenic Mouse Models
Primary Culture and Live Imaging of Adult Neural Stem Cells and Their Progeny
Live Imaging, Identifying, and Tracking Single Cells in Complex Populations In Vivo and Ex Vivo
结缔组织类细胞培养
Freeze-Etch Electron Tomography for the Plasma Membrane Interface
Local Application of Bone Morphogenic Protein on Developing Chick Embryos Using Fibrous Glass Matrix
Assessment of Receptor Internalization and Recycling
自我剪接(self-splicing)
Nonradioactive Northern Blotting
Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting
小鼠5羟色胺(5-HT)酶联免疫分析（ELISA）
Quantification of Poly(ADP-Ribose) In Vitro: Determination of the ADP-Ribose Chain Length and Branch
Microexplant Cultures of the Cerebellum
Microexplant Cultures of the Cerebellum
Computer-Assisted Systems for the Analysis of Amoeboid Cell Motility
Culturing and Differentiating Human Mesenchymal Stem Cells for Biocompatible Scaffolds in Regenerati
Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration
Time-Lapse Fluorescence Microscopy of Saccharomyces cerevisiae in Meiosis
Binding Affinity and Kinetic Analysis of Nuclear Receptor/Co-Regulator Interactions Using Surface Pl
Clot Retraction
Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases
Adipogenic Differentiation of Human Induced Pluripotent Stem Cells
Frster Resonance Energy Transfer-Based Imaging at the Cell Surface of Live Cells
Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay
The Isolation of Membranes from Bacteria
G带(Giemsa-banding)
Simultaneous Real-Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
Monitoring Spindle Assembly and Disassembly in Yeast by Indirect Immunofluorescence
Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells
Transplantation of Embryonic Stem Cell-Derived Dopaminergic Neurons in MPTP-Treated Monkeys
Immunoblotting 2DE Membranes
Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease
同源异型基因（homeotic gene）
Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissue
Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors
Continuous-Flow Electrophoresis: Application to the Isolation of Lysosomes and Endosomes
Cos-1细胞暂时转染protocol
Protocol to Enrich and Analyze Plasma Membrane Proteins
Immunohistochemical Detection of Tumour Hypoxia
Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic
Preservation Protocols for Human Adipose Tissue-Derived Adult Stem Cells
Assessing Aging and Senescent Decline in Caenorhabditis elegans: Cohort Survival Analysis
Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas
Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency
Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes
Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics
Myoglobin Analysis
A Review of the Methods for Human iPSC Derivation
抗氧剂BHT知识百科
Methods for the Expression, Purification, Preparation, and Biophysical Characterization of Construct
人血管内皮细胞粘附分子1 (VCAM-1)酶联免疫分析（ELISA）
Plasma Polymer and PEG-Based Coatings for DNA, Protein and Cell Microarrays
Array CGH: Opening New Horizons
A pH-Indicator-Based Screen for Hydrolytic Haloalkane Dehalogenase
Microisolation and Microanalysis of Plasmid DNA
Culture of Human Embryonic Stem Cells (hESC)
Purification and Depletion of RNP Particles by Antisense Affinity Chromatography
Site-Directed Mutagenesis In Vitro by Megaprimer PCR
Models for Gain-of-Function and Loss-of-Function of MMPs: Transgenic and Gene Targeted Mice
Prenatal Diagnostics on Uncultured Amniocytes
Methods for Creating Mutations in C. elegans That Extend Lifespan
Mouse Models of Viral Infection: Influenza Infection in the Lung
Freezing and Thawing cells
Adult Cell Fate Reprogramming: Converting Liver to Pancreas
Molecular Modeling and Molecular Dynamics Simulations of Membrane Transporter Proteins
Oral and Pharyngeal Epithelial Keratinocyte Culture
Analysis of Change
Immunoglobulin Class Switching: In Vitro Induction and Analysis
Gene Delivery to Mesenchymal Stem Cells
Preparation of Template DNA and Labeling Techniques
细胞遗传学(cytogenetics)
胆固醇(cholesterol)
Monitoring Nutrient Signaling Through the Longevity Protein p66SHC1
Regeneration of Skin and Cornea by Tissue Engineering
Measurement of Changes in Intracellular Calcium During Apoptosis
Monitoring the Cycling Activity of Cultured Human Keratinocytes Using a CFSE-Based Dye Tracking Appr
The Evolution of Mass Spectrometry in the Clinical Laboratory
大鼠血小板活化因子（PAF）酶联免疫分析（ELISA）
Activation of SAPK/JNKs In Vitro
One-Tube RT-PCR with Sequence-Specific Primers
The GAL4 System: A Versatile System for the Expression of Genes
The Corneal Pocket Assay
Preparation of Efficient Splicing Extracts From Whole Cells, Nuclei, and Cytoplasmic Fractions
Choosing an Adipose Tissue Depot for Sampling: Factors in Selection and Depot Specificity
Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick Translation
Adipose-Derived Stem Cells
Strategies for Cloning New MMPs and TIMPs
Infection with retroviruses
Imaging Calcium Sparks in Cardiac Myocytes
Introduction to Nuclear Magnetic Resonance
Nestin-Driven Green Fluorescent Protein as an Imaging Marker for Nascent Blood Vessels in Mouse Mode
Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases
Quantification of Lysophosphatidylcholine Species by High-Throughput Electrospray Ionization Tandem
Mechanisms of Neurulation
Isolation and Characterization of the Heat Shock RNA 1
Pre-embedding Electron Microscopy Methods for Glycan Localization in Chemically Fixed Mammalian Tiss
3D Cell Culture: A Review of Current Approaches and Techniques
Genetic Fate-Mapping for Studying Adult Cardiomyocyte Replenishment After Myocardial Injury
Chromatin Immunoprecipitation
Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements During Xenopu
细胞移动(cell locomotion)
Cell Motility and Invasion Assays
3D Structuring of Biocompatible and Biodegradable Polymers Via Stereolithography
Islet-Derived Progenitors as a Source of In Vitro Islet Regeneration
Mesoderm Induction in Xenopus: Oocyte Expression System and Animal Cap Assay
Assays for Vertebrate Sperm Chemotaxis
Isolation and Culture of Human Hepatocytes
内膜(inner membrane)
Detection of Mycoplasma Contaminations
Abnbio IMDM培养基含L-谷氨酰胺 HEPES 不含α-硫代甘油 A124405 500ml
Xenogeneic Lung Transplantation Models
Atomic Force Microscopy Measurements of Intermolecular Binding Forces
Parental Origin Determination FISH: Pod-FISH
E位点(exit site, E site)
Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multipa
Transmembrane Signaling by Receptor Oligomerization
Isolation and Culture of Bovine Embryonic Stem Cells
Isolation of Cytokine-Inducible Genes from Hematopoietic Cells by Differential Display
胞间连丝(plasmodesmata)
Therapeutic Uses of Antioxidant Liposomes
Analysis of Changes in the Expression Pattern of Claudins Using Salivary Acinar Cells in Primary Cul
Carboxyfluorescein as a Marker at Both Light and Electron Microscope Levels to Follow Cell Lineage i
Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Si
Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay
光反应中心复合物(reaction-center complex)
DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules
Direct Labeling of Components in Protein Complexes by Immuno-Electron Microscopy
Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry
Defining Hormone and Matrix Requirements for Differentiated Epithelia
An Integrative Procedure for Apoptosis Identification and Measurement
DNA Methylation Analysis of Germ Cells by Using Bisulfite-Based Sequencing Methods
In Vivo Mechanical Loading
有丝分裂器(mitotic apparatus)
Ultrastructural Analysis of Testicular Tissue and Sperm by Transmission and Scanning Electron Micros
Assaying In Vitro Refolding of RNases by Mass Spectrometry
细胞通讯(cell communication)
G418筛选稳定表达细胞系
Statistical Analysis of Biological Rhythm Data
Isolation of c-Kit+ Human Amniotic Fluid Stem Cells from Second Trimester
Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Ch
Detection of DNA Strand Breakage in the Analysis of Apoptosis and Cell Proliferation by Flow and Las
Approaches to Separate and Identify PolyADP-Ribosylated Proteins Using Poly(ADP-Ribose) Glycohydrola
Ras and Rac as Activators of Reactive Oxygen Species (ROS)
Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation
Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR
Fertilization In Vitro
Stable Integration of Large PAC Constructs in Keratinocytes
Northwestern Screening of Expression Libraries
Analysis of Cell Migration Using Drosophila as a Model System
Sequence Analyses to Study the Evolutionary History and Cis-Regulatory Elements of Hedgehog Genes
Assessing Protein Synthesis by Platelets
Measuring Permeability in Human Retinal Epithelial Cells (ARPE-19): Implications for the Study of Di
Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells
Studying Wnt Signaling in Xenopus
Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and Their Applicat
3H-Thymidine Uptake by Cultured Cells
Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells
着丝点
欧氏平衡盐(粉末型)成分
Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ In Vitro
Chondrogenic Differentiation of hESC in Micromass Culture
Use of I-Sce I to Induce DNA Double-Strand Breaks in Nicotiana
变态(metamorphosis)
Assays for Detection of RNase A Superfamily Ribonucleases
Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells
鬼笔环肽(phalloidin)
RNAi Knockdown of Redox Signaling Protein Ape1 in the Differentiation of Mouse Embryonic Stem Cells
Chromatin Immunoprecipitation Assay for Mammalian Tissues
Consequences of Stress in the Secretary Pathway: The ER Stress Response and Its Role in the Metaboli
你知道基础培养基的成分么？
Cultivation of Keratinocytes from the Outer Root Sheath of Human Hair Follicles
Progress Towards Drosophila Epithelial Cell Culture
Vitrifying and Warming of Human Oocytes, Embryos, and Blastocysts: Vitrification Procedures as an Al
Assessing G1-to-S-Phase Progression After Genotoxic Stress
Bcl-2蛋白(Bcl-2 protein)
Hematopoietic Stem Cell Mobilization with Agents Other than G-CSF
Biosynthesis of Proteins Inside Liposomes
二联管(doublet)
Proteomics of Yeast Mitochondria
Single-Cell Calcium Imaging
Tandem Affinity Purification and Identification of Heterotrimeric G Protein-Associated Proteins
The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients
Quantifying In Vivo Phosphoinositide Turnover in Chemotactically Competent Dictyostelium Cells
A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Seque
In Vitro Studies on Odontogenic Tumors
Paraoxonases (PON1, PON2, PON3) Analyses In Vitro and In Vivo in Relation to Cardiovascular Diseases
WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells
Serum Protein Electrophoresis
Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans
Quantification of Cells in Culture
Noncovalent pADPr Interaction with Proteins and Competition with RNA for Binding to Proteins
Generation of Induced Pluripotent Stem Cells from Human Amnion Cells
Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U
Photoactivatable-GFP--Tubulin as a Tool to Study Microtubule Plus-End Turnover in Living Human Cells
细胞生物学(cell biology)
流式细胞仪检测外周血树突状细胞
小鼠海马神经元培养
Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System
Whole Embryo Imaging of Hematopoietic Cell Emergence and Migration
Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes
Methods for Double Detection of Gene Expression: Combined In Situ Hybridization and Immunocytochemis
Caenorhabditis elegans as a Model System for Triplet Repeat Diseases
Cell Sheet Technology for Tissue Engineering: The Self-Assembly Approach Using Adipose-Derived Strom
Mammalian Preimplantation Embryo Culture
Conjunctiva Organ and Cell Culture
Serial Transplantation of Bone Marrow to Test Self-renewal Capacity of Hematopoietic Stem Cells In V
A First Approach for the Production of Human Adipose Tissue-Derived Stromal Cells for Therapeutic Us
受体交叉(receptor crossover)
A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays
细胞决定(cell determination)
Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members
Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Funct
Assaying Protein Kinase Activity
What Role Does Mitochondrial Stress Play in Neurodegenerative Diseases
Mouse Chimeras and the Analysis of Development
Ethical and Regulatory Issues for Clinical Trials in Xenotransplantation
Analysis of Germline Chromatin Silencing by Double-Stranded RNA-Mediated Interference (RNAi) in Caen
Bisulfite
Preparation of Human Trophoblast Cells for Culture
稳定转染攻略：从G418浓度确定到单克隆化鉴定
Organ Culture of Developing Mouse Skin and Its Application for Molecular Mechanistic Studies of Morp
细胞培养实验用品
Preparation of Mammalian Oocytes for Transmission Electron Microscopy
Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays
Polyamine Block of Inwardly Rectifying Potassium Channels
Sage: The Serial Analysis of Gene Expression
X-Ray Crystallography of Agonist/Antagonist-Bound Receptors
The Development of T Lymphocytes in Fetal Thymus Organ Culture
Exometabolomic Mapping of Caenorhabditis elegans: A Tool to Noninvasively Investigate Aging
Isolation, Cultivation, and Application of Human Alveolar Epithelial Cells
Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Mo
The Nuclear UbiquitinProteasome System: Visualization of Proteasomes, Protein Aggregates, and Proteo
Human Osteoclast Culture From Peripheral Blood Monocytes: Phenotypic Characterization and Quantitati
Capillary Electrophoresis of Sugar Acids
大鼠环磷酸腺苷(cAMP)酶联免疫分析
细胞质膜系统(cytoplasmic membrane system)
Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation
Primary Cultures of Rat Hepatocytes
Isolation of Cutaneous Dendritic Cells
Bioassays of Inductive Interactions in Amphibian Development
Evaluation of Antifungal Susceptibility Using Flow Cytometry
How to Measure Microtubule Dynamics
In Vitro Maturation and Fertilization of Canine Oocytes
Measuring Reactive Oxygen Species in Senescent Cells
Preclinical Testing of Virotherapeutics for Primary and Secondary Tumors of the Liver
Optimized Fixation and Immunofluorescence Staining Methods for Dictyostelium Cells
Zebrafish Immunohistochemistry
Differentiate ES cells into cardiac myocytes
Quantitation of Fentanyl in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)
Combined Bidimensional Electrophoresis and Electron Microscopy to Study Specific Plasmid DNA Replica
Derivation and Characterization of Hepatocytes From Embryonic Stem Cells In Vitro
Intravenous Human Mesenchymal Stem Cells Transplantation in NOD/SCID Mice Preserve Liver Integrity o
Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging
Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells
Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes
Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases
Quantitation of Indomethacin in Serum and Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS)
In Situ Hybridization to Polytene Chromosomes
被膜小窝(clathrin-coated pit)
Differential Screening for the Isolation of Species-Specific Sequences in the Anopheles farauti Comp
精原细胞（spermatogonia）
Practical Considerations for the Analysis of Ionic and Neutral Organic Molecules With Capillary Elec
An Efficient Way of Studying ProteinProtein Interactions Involving HIF-, c-Myc, and Sp1
Neural Crest Migration Methods in the Chicken Embryo
关于细胞培养中的污染
Hepatocyte Xenotransplantation
Quantitative In Situ Hybridization of Tissue Microarrays
Histochemical Localization of Cell Proliferation Using In Situ Hybridization for Histone mRNA
Quantitation of Ibuprofen in Blood Using Gas Chromatography-Mass Spectrometry (GC-MS)
Endothelial Cell Systems - Instructions For Use
红细胞膜的制备及其成份分析(磷脂、胆固醇)
Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentration
NF-Ya Protein Delivery as a Tool for Hematopoietic Progenitor Cell Expansion
Transplantation of Neural Stem/Progenitor Cells into Developing and Adult CNS
In Vivo Quantification of Metastatic Tumor Cell Adhesion in the Pulmonary Microvasculature
Studying Colloidal Aggregation Using Liposomes
Measurement of Mitochondrial Oxygen Consumption Using a Clark Electrode
Cytological Techniques to Study Human Female Meiotic Prophase
Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells
Immunoelectron Microscopy of Cryofixed Freeze-Substituted Saccharomyces cerevisiae
Mechanistic Studies of Skin Electroporation Using Biophysical Methods
Methods to Study Protein-Protein Interactions
Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones
Strategies Employed for the Development of PARP Inhibitors
果胶(pectin)
Yeast Two-Hybrid Screening
High Activity Preparations of Lipases and Proteases for Catalysis in Low Water Containing Organic So
$SDS Gel Electrophoresis of Tubulin\MAPs$
SDS Gel Electrophoresis of Tubulin\MAPs
Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods
Active Protease Mapping in 2DE Gels
Quantitation of Acute Necrosis After Experimental Myocardial Infarction
人胃动素(MTL)酶联免疫分析（ELISA）
Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors
The Chimeric Human/Mouse Model of Angiogenesis
Analysis of Microtubule-Mediated Intracellular Viral Transport
Nonradioactive In Situ Hybridization on Frozen Sections and Whole Mounts
Real-Time Visualization and Quantification of Native Ras Activation in Single Living Cells
Neuroepithelial Differentiation Induced by ECM Molecules
Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules
Analysis of the Degradome with the CLIP-CHIP Microarray
Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma
Genome Mapping Statistics and Bioinformatics
Detection of Intracellular/lntranuclear Antigens: Applications in Leukemia/Lymphoma Analysis
Disc Angiogenesis Assay
Circulating Endothelial Progenitor Cells (CEPC)
人肝细胞生长因子受体（HGFR/ c-MET）酶联免疫分析（ELISA）
冰冻蚀刻(freeze-etching)技术
遗传信息表达结构系统(genetic expression system)
细胞培养用血清保存方法与血清解冻常见问题
Preparation of NeoR Murine Embryonic Fibroblasts
从单个核细胞中分离出T细胞
稳定转染之后细胞荧光强度差异很大
确认过眼神，遇见「物美价廉的」示踪细胞株
Immunofluorescent Staining of Mouse and Rat Leukocytes
免疫荧光样品制备与观察
Detection In Vitro and Quantitative Estimation of Artificial Microterritories Which Promote Osteogen
DelsGate: A Robust and Rapid Method for Gene Deletion
Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells
The CGG Repeat and the FMR1 Gene
Antisense Therapy in Clinical Oncology: Preclinical and Clinical Experiences
Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Hum
Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled
Mutant Generation in Vertebrate Model Organisms by TILLING
Labeling Primitive Myeloid Progenitor Cells in Xenopus
Proliferation Assay: [3H] Thymidine incorporation
Microbial Molecular Epidemiology: An Overview
Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays
Collection, Processing, and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regen
Analysis of DNA Damage and Repair by Comet Fluorescence In Situ Hybridization (Comet-FISH)
Enrichment of Oral Mucosa and Skin Keratinocyte Progenitor/Stem Cells
Human Fetal Brain Cell Culture
Culturing Primitive Hemopoietic Cells: Long-Term Mouse Marrow Cultures and the Establishment of Fact
Immunoassay Measurements of Leptin
Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA)
Methods for Studying Vinca Alkaloid Interactions With Tubulin
Palatal Dysmorphogenesis: Palate Organ Culture
Generating Neurons from Stem Cells
Cardiac Stem Cell Niche, MMP9, and Culture and Differentiation of Embryonic Stem Cells
Induction of Intracellular Signals Through Binding of Adhesive Molecules: Activation of p125FAK Tyro
Whole-Mount Imaging of Responses in Mouse Vomeronasal Neurons
Poly(Carbamoyl Sulfonate) Hydrogels
Activity of MAPK/p90rsk During Fertilization in Mice, Rats, and Pigs
Bovine Retinal Microvascular Pericytes: Isolation, Propagation, and Identification
Saccharomyces cerevisiae as a Model Organism to Study Mitochondrial Biology: General Considerations
Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers
General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds
Embryo/Fetal Topographical Analysis by Fluorescence Microscopy and Confocal Laser Scanning Microscop
Enzymatic Isolation and Serum-Free Culture of Human Renal Cells: Retaining Properties of Proximal Tu
Examining Planar Cell Polarity in the Mammalian Cochlea
Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized L
Assay of Deoxyhypusine Hydroxylase Activity
Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes
Integration of Protein Kinases into Transcription Complexes: Identifying Components of Immobilised I
The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair
Determination of the Activity of Rho-Like GTPases in Cells
Methods to Assess Tissue Permeability
自体组装(self assembly)
核糖体
Nucleotide Metabolism and Cell-Cell Interactions
FISH on Sperms, Spermatocytes and Oocytes
细胞转染方法
Tissue Culture Media
减数分裂
QUALITATIVE ANALYSIS OF DNA FRAGMENTATION BY AGAROSE GEL ELECTROPHORESIS
Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry
Isolation of Murine Adipose-Derived Stem Cells
Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes
Preparation of Isolated Periportal or Perivenous Hepatocytes from Rat Liver
Isolation and Growth of Adipose Tissue-Derived Stem Cells
In Vitro Clonal Culture of Human Hematopoietic Progenitor Cells
Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis
High Throughput Analysis of Nuclear ReceptorCofactor Interactions
Mechanical Stimulation of Bone Cells Using Fluid Flow
高通量细胞筛选技术(high throughput cell-based screening technology)
The Immunodetection of pADPr in Drosophila Tissues: Immunostaining of Thick Sections; Immuno-EM
RNA-Protein Crosslinking with Photoreactive Nucleotide Analogs
Methods to Study Transcription-Coupled Repair in Chromatin
Preparation of Human Partial Chromosome Paints from Somatic Cell Hybrids
造血干细胞移植（HSCT）的适应症
Determination of Unknown Genomic Sequences Without Cloning
Drosophila as a Genetic Tool to Define Vertebrate Pathway Players
Microbodies
Serum-Free Culture of Mid-gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs
Methods for the Study of Redox-Mediated Changes in p53 Structure and Function
Rat Aortic Ring: 3D Model of Angiogenesis In Vitro
PCR产物直接转染细胞并转录siRNA说明
Characterizing ECM Production by Cells Encapsulated in Hydrogels
Studying Head and Brain Development in Drosophila
Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets
Troubleshooting Image Analysis in 2DE
Mouse Hematopoietic Stem Cell Transplantation
Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth
Spermidine/Spermine-N1-Acetyltransferase in Kidney Ischemia Reperfusion Injury
MAP Kinase Activation by Receptor Tyrosine Kinases: In Control of Cell Migration
Plant Protein Extraction
In Vitro Ubiquitination of Cytokine Signaling Components
In Vitro Maturation and Embryo Production in Cattle
Therapeutic Inhibition of Angiogenesis
Membrane Protein Expression in Lactococcus lactis
Assessment of Spermatogenesis Through Staging of Seminiferous Tubules
Fluorescence Recovery After Photobleaching (FRAP) to Study Nuclear Protein Dynamics in Living Cells
Maturation Changes in Purified Phagosomes
大鼠抗缪勒管激素(AMH)酶联免疫分析（ELISA）
Isolation Procedure and Characterization of Multipotent Adult Progenitor Cells from Rat Bone Marrow
High-Resolution Large-Gel 2DE
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secr
In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
The Application of Randomly Amplified DNA Analysis in the Molecular Epidemiology of Microorganisms
Isolation, Growth, and Characterization of Human Renal Epithelial Cells Using Traditional and 3D Met
Ubiquitination of G-Protein-Coupled Receptors
Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates
Analysis of Triplet Repeat Replication by Two-Dimensional Gel Electrophoresis
杀灭黑胶虫的一种方法
Released Chromatin or DNA Fiber Preparations for High-Resolution Fiber FISH
Data Analysis in Cell Kinetics Research
Retroviral Vector-Based Approaches for the Generation of Human Induced Pluripotent Stem Cells from F
Chromosome Sorting and Analysis by FACS
Identification of Genes Associated with Resistance to Photodynamic Therapy-Mediated Oxidative Stress
An In Vitro System for Measuring the Transdermal Voltage and Molecular Flux Across the Skin in Real
Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes
Biochemical Methods Used to Study the Gene Expression and Protein Complexes in the Filamentous Fungu
分子发动机(molecular motor)
Detecting Polymorphisms in MMP Genes
Expression of Epitope-Tagged Proteins in Plants
Cell Sorting of Young and Senescent Cells
Assaying Lipid Phosphate Phosphatase Activities
Analysis of PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays
Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time
Measurements of Active TGF Generated by Culture Cells
Membrane Translocation Assayed by Fluorescence Spectroscopy
单管(singlet)
Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells
Mammalian Fertilization Is Dependent on Multiple Membrane Fusion Events*
Modeling Brain Pathologies Using Neural Stem Cells
Direct Ex Vivo Activation of T cells for Analysis of Dendritic Cells Antigen Presentation
G Protein-Coupled Receptor Dimerization and Signaling
Pheromones in the Fruit Fly
Mobilization of Hematopoietic Stem Cells by Depleting Bone Marrow Macrophages
Separation of Labeled Inositol Phosphate Isomers by High-Pressure Liquid Chromatography (HPLC)
Immunocytochemical Analysis of Neuronal Differentiation
The Role of Hex in Hemangioblast and Hematopoietic Development
The Binding of Protein-Ligands to Cell-Surface Receptors
In Vivo Visualization of RNA Using the U1A-Based Tagged RNA System
Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mapping
Alginate Microbead Release Assay of Angiogenesis
Single-Cell Culture in Microwells
Bioinformatic Approaches to the Identification of Novel Neuropeptide Precursors
Quantitative Fluorescence Microscopy Techniques
Practical Aspects of OCT Imaging in Tissue Engineering
Automated Planar Patch-Clamp
Closed Ampoule Isothermal Microcalorimetry for Continuous Real-Time Detection and Evaluation of Cult
Mapping of Protein Transduction Pathways with Fluorescent Microscopy
Recombinant Adeno-Associated Viral Vector Production and Purification
大鼠半乳糖凝集素-9酶联免疫分析（ELISA）
Preparation of a Multi-antioxidant Formulation
信号转导(signal transduction)
Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In
Isolation and Culture of Primary Endothelial Cells
Zymography and Reverse Zymography for Detecting MMPs and TIMPs
The Mitochondria of Cultured Mammalian Cells: II: Expression and Visualization of Exogenous Proteins
Quantitation of Amobarbital, Butalbital, Pentobarbital, Phenobarbital, and Secobarbital in Urine, Se
Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells
Testicular Germ Cell Tumors in Mice: New Ways to Study a Genetically Complex Trait
In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells
Cloning of Cytokine 3 Untranslated Regions and Posttranscriptional Assessment Using Cell-Based GFP A
Reporter/Functional Gene Transfer in Rat Brain
Looking for the Niche: Substance Delivery into the Lateral Ventricle of the Brain: The Osmotic Minip
The Zebrafish: An Overview of Its Early Development
The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations
Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-Ray Crystallography
The Basic-Helix-Loop-Helix-Leucine Zipper Gene Mitf: Analysis of Alternative Promoter Choice and Spl
Histochemistry and Lipid Profiling Combine for Insights into Aging and Age-Related Maculopathy
Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase
Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Poly
Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins
单克隆抗体的制备与鉴定方法
Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
Glomerular Epithelial and Mesangial Cell Culture and Characterization
Matrix Assembly, Cell Polarization, and Cell Survival: Analysis of Peri-Implantation Development Wit
Isolation and Purification Method of Mouse Fetal Hepatoblasts
Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria
Assays of Lipolytic Enzymes
Preparing Mitochondria from Rat Liver
Isolation and Culture of Bone-Forming Cells (Osteoblasts) from Human Bone
Tracking Nanoparticles in Three-Dimensional Tissue-Engineered Models Using Confocal Laser Scanning M
分子细胞生物学(molecular biology of the cell)
细胞死亡(cell death)
ChIP on Chip Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications
兔白细胞介素6（IL-6）酶联免疫分析（ELISA）
Processing mRNA 3' Ends In Vitro
Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord
Mapping the Lipolytic Proteome of Adipose Tissue Using Fluorescent Suicide Inhibitors
Gene Targeting by Oligonucleotides in Keratinocytes
Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstal
Study of Skeletal Myogenesis in Cultures of Unsegmented Paraxial Mesoderm
Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)
Broad Spectrum Drug Screening Using Electron-Ionization Gas Chromatography-Mass Spectrometry (EI-GCM
Use of Escherichia coli for the Production and Purification of Membrane Proteins
Isolation of Mitochondria From Procyclic Trypanosoma brucei
Engineering Elements for Gene Silencing: The Artificial MicroRNAs Technology
The Stem Cell Laboratory: Design, Equipment, and Oversight
Direct Sequencing by Thermal Asymmetric PCR
Fluorescent Glycerolipid Probes: Synthesis and Use for Examining Intracellular Lipid Trafficking
Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR
Isolation of Sperm Nuclei and Nuclear Matrices from the Mouse, and Other Rodents
Monitoring of the Dynamics of Epithelial Dome Formation Using a Novel Culture Chamber for Long-Term
分子生物学的三部分研究内容
Numerical Modeling for In Vivo Electroporation
Profiling Axonal mRNA Transport
内膜系统(endomembrane systems)
大鼠白细胞介素2（IL-2）酶联免疫分析
Survival Analysis
Viability Cell Count
Dissecting Mesenchymal Stem Cell Movement: Migration Assays for Tracing and Deducing Cell Migration
Detection and Isolation of Trinucleotide Repeat Expansions Using the RED Method
In Vitro Selection of Allosteric Ribozymes
Keratocyte Cell Culture
Anti-claudin-4-Conjugated Highly Luminescent Nanoparticles as Biological Labels for Pancreatic Cance
Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells
The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluoresc
The Purification of Mouse Hematopoietic Stem Cells at Sequential Stages of Maturation
Electrochemical-Based Immobilization of Enzymes
Measuring Proteolysis in Mitosis
Immunizing Schedules for Hybridoma Production
Stem Cells for Myocardial Repair and Regeneration: Where Are We Today
Retroviral delivery of ECM genes
Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells
A Method to Visualize the Actin and Microtubule Cytoskeleton by Indirect Immunofluorescence
Measurement of Cell-Penetrating Peptide-Mediated Transduction of Adult Hematopoietic Stem Cells
Database Setup for Preclinical Studies of Gene-Modified Hematopoiesis
Suprachiasmatic Nucleus Cultures That Maintain Rhythmic Properties In Vitro
Immobilization of Enzymes on Microelectrodes Using Chemical Crosslinking
Selective Detection of Phagocytic Phase of Apoptosis in Fixed Tissue Sections
Determination of mRNA and Protein Expression Patterns in Zebrafish
Live-Cell Fluorescence Imaging of Meiotic Chromosome Dynamics in Schizosaccharomyces pombe
Ratiometric Ca2+ Measurements Using the FlexStation Scanning Fluorometer
Methods to Measure Membrane Potential and Permeability Transition in the Mitochondria During Apoptos
Experimental Identification of MicroRNA Targets by Immunoprecipitation of Argonaute Protein Complexe
Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryoni
Immobilization of Enzymes and Cells: Some Practical Considerations
Structural Analysis of Protein-RNA Interactions With Mass Spectrometry
关于动物血清的常见问题及处理方法
Chemical Assays for Proteins
Generation of Human-Induced Pluripotent Stem Cells (hiPSCs) Using Episomal Vectors on Defined Essent
Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a
Introduction to Nucleocytoplasmic Transport: Molecules and Mechanisms
Analysis of Biomarkers of Caloric Restriction in Aging Cells
常用封闭剂选择的窍门
Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and
Identification of Mutated p53 in Cancers by Nongel-Sieving Capillary Electrophoretic SSCP Analysis
Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture
矽肺(silicosis)
Activation of the Mitogen-Activated Protein Kinase Pathway by TGF
Detection of the Senescence-Associated Secretory Phenotype (SASP)
Stallion Spermatozoa Viability: Comparison of Flow Cytometry with Other Methods
Measurements of Stathmin-Tubulin Interaction in Solution
Biochemical Responses to Chemoattractants in Dictyostelium: Ligand-Receptor Interactions and Downstr
Analysis of Glycosaminoglycans in Stem Cell Glycomics
Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells
Antisense Oligonucleotide Treatment of Inflammatory Bowel Diseases
The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Prote
Quantitation of Cellular and Topical Uptake of LuciferinOligoarginine Conjugates
A Cellular Viability Assay to Monitor Drug Toxicity
The Extraction and Analysis of Glycosphingolipids
Mechanical Properties of Interphase Nuclei Probed by Cellular Strain Application
Studying the Function of Dendritic Cells in Mouse Models of Asthma
Transwell® 通透性支持物的选择和使用手册
The Sponge Implant Model of Angiogenesis
Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells
Antibodies to MMP-Cleaved Aggrecan
Separation of Platelets from Whole Blood
Assay of Cell Cycle Kinetics by Multivariate Flow Cytometry Using the Principle of Stathmokinesis
分子生物学(molecular biology)
Biomedical Applications of Amino Acid Detection by Capillary Electrophoresis
Acetonitrile Stacking: Serum Phenobarbital as an Example
Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning
Imaging Pluripotent Cell Migration in Drosophila
In Vivo and Ex Vivo Gene Transfer in Thymocytes and Thymocyte Precursors
FISH in Food Microbiology
Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models
Micelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase Assays
Peptidomic Approaches to the Identification and Characterization of Functional Peptides in Hydra
Computational Modeling of Signaling Networks for Eukaryotic Chemosensing
Characterization of DNA Condensates by Atomic Force Microscopy
HGF-Induced DU145 Cell Scatter Assay
Using Organotypic Tissue Slices as Substrata for the Culture of Dissociated Cells
Assessment of In Vitro and In Vivo Mitochondrial Function in Friedreich’s Ataxia and Huntington’s Di
Ultracentrifugation Technique for Measuring the Binding of Peptides and Proteins to Sucrose-Loaded P
Mass Measurement of Key Phosphoinositide Cycle Intermediates
Basic Protocols to Investigate hMSC Behavior onto Electrospun Fibers
Isolation of Sea Urchin Sperm Plasma Membranes
Human Thyroid Epithelial Cells
A High-Throughput Sequencing-Based Methodology to Identify All Uncapped and Cleaved RNA Molecules in
Flow Cytometry: Immunofluorescence Staining of Activated and Rest
Using TUNEL in Combination with an Active Caspase-3 Immunoassay to Identify Cells Undergoing Apoptos
Isolation of Human Blood DC Subtypes
Methods for the Analysis of the Sperm Proteome
Pig Neural Cells Derived from Foetal Mesencephalon as Cell Source for Intracerebral Xenotransplantat
Freezing Cells
Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches
The Chick Embryo as a Model System for Analyzing Mechanisms of Development
Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloprotei
Transcriptome Analysis of Drosophila Neural Stem Cells
Isolation of Total RNA from Tissues or Cell Lines: Visualization in Gel
Generation of Protease-Deficient Strains and Their Use in Heterologous Protein Expression
Flow Cytometric Detection of Fluorescent Redistributional Dyes for Measurement of Cell Transmembrane
Epidermal Permeability Barrier Measurement in Mammalian Skin
奥林巴斯倒置显微镜CKX-31操作和维护说明
Microsequencing of Myosins for PCR Primer Design
A Detailed Protocol for Bacterial Artificial Chromosome Recombineering to Study Essential Genes in S
Monitoring DNA Damage During Cell Senescence
Negative Stain Electron Microscopy of Microtubules
Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1
Utilizing DNA Mutations to Trace Epithelial Cell Lineages in Human Tissues
In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages
Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene
Production of Tissue-Engineered Skin and Oral Mucosa for Clinical and Experimental Use
细胞培养中几个错误认识
Monoclonal Antibodies Against Glycosphingolipids (GSLs)-Gangliosides
Primary Culture of Ovarian Cells for Research on Cell Interactions in the Hormonal Control of Steroi
Isolation and Establishment of Hair Follicle Dermal Papilla Cell Cultures
Assessing the Function of mTOR in Human Embryonic Stem Cells
Vitrification of Plant Cell Suspensions
Media Composition: Growth Factors
Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Heterodimerization
Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Chorion, Amniocytes, and Fibroblast
In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors
Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers
Bioinformatic Approaches to Metabolic Pathways Analysis
Use of Capillary Electrophoresis for Polysaccharide Studies and Applications
减数分裂(meiosis)
Immobilized Culture and Transfection Microarray of Non-adherent Cells
Use of Doppler Echocardiography to Monitor Embryonic Mouse Heart Function
Identification of Apoptosis Regulatory Genes Using Insertional Mutagenesis
选择合适的标记方法
Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing
Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays
Measuring Inflammatory Cell Migration in the Zebrafish
姊妹染色单体交换实验
Isolation of Adult Stem Cells and Their Differentiation to Schwann Cells
Gene Targeting in Cultured Human Cells
Magnetic Resonance Imaging and Tracking of Stem Cells
DNA Labeling In Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Fo
微管（microtube）
The Culture and Maintenance of Functional Retinal Pigment Epithelial Monolayers from Adult Human Eye
High-Throughput and Longitudinal Analysis of Aging and Senescent Decline in Caenorhabditis elegans
Therapeutic Applications of Cell-Penetrating Peptides
Using siRNA Knockdown in HaCaT Cells to Study Transcriptional Control of Epidermal Proliferation Pot
小牛血清和胎牛血清的区别
Measuring Transcription of Metalloproteinase Genes: Nuclear Run-Off Assay vs Analysis of hnRNA
Three-Color FISH for the Detection of Individual Radiosensitivity
美国科学家发现一种用处极大的细菌
Genetic Modification of Mesenchymal Stem Cells
Whole Mount Immunohistochemistry and In Situ Hybridization of Larval and Adult Zebrafish Dental Tiss
Analysis of Epithelial Barrier Integrity in Polarized Lung Epithelial Cells
Use of Gene Targeting to Study Recombination in Mammalian DNA Repair Mutants
In Vitro Repair Model of Focal Articular Cartilage Defects in Humans
Telomere Neurobiology
Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers
Aggregating Neural Cell Cultures
Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45 Ca2+ and Fluo-3
血清热灭活的问题
Culturing HEK 293 Cells
微生物污染的排除
The Use of Consensus PCR and Direct Sequence Analysis for the Identification of HPV
Hematopoietic Colony-Forming Cells
The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes: Transcrip
C4途径(C4 pathway)
T cell Activation Protocol
Confocal Laser Scanning Microscopy Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos
ELISPOT (Enzyme-linked ImmunoSPOT)
三羧酸循环(citric acid cycle)
细胞培养中的黑胶虫污染问题的处理
The Cell Environment (including types of culture medium)
杂交瘤细胞的制备方法
红细胞裂解的实验方法步骤
Craniofacial Development of Avian and Rodent Embryos
Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation S
去壁低渗法制备植物染色体标本实验
Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
In Situ Detection of EpsteinBarr Virus and Phenotype Determination of EBV-Infected Cells
The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression
Single-Cell Enzymatic Dissociation of hESC Lines OxF1OxF4 and Culture in Feeder-Free Conditions
Cytotoxicity Assays Protocol
粗面内质网(rough endoplasmic reticulum, RER)
Fluorescence Microscopy of Microtubules in Cultured Cells
Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Bl
Study of Respiratory Cytochromes in Liposomes
Primary Cell Culture of Human Enteric Neurons: Submucosal Plexus
A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplas
Belcher/Knight: Electrocompetent Cells
In Situ PCR Detection of HIV Expression in the Human Placenta
细胞组分和细胞器――细胞膜
In Vitro Reprogramming of Pancreatic Cells to Hepatocytes
High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis
Assays of Bypass Replication of Genotoxic Lesions in Mammalian Disease and Mutant Cell-Free Extracts
Defining Pre-mRNA cis Elements that Regulate Cell-Specific Splicing
DNA Microarrays to Analyze Gene Expression in Animals with Altered Transporter Expression
小鼠白细胞介素-35（IL-35）酶联免疫分析（ELISA）
电穿孔与电融合技术
293细胞的大规模培养的方法
Cellularity Measurements
Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenet
Transdermal Drug Delivery by Skin Electroporation in the Rat
Retroviral-Mediated Transduction and Clonal Integration Analysis of Human Hematopoietic Stem and Pro
Quantification of MicroRNAs, Splicing Isoforms, and Homologous mRNAs With the Invader Assay
动物细胞大规模培养用生物反应器简介
Functional Analysis of Nocturnin: A Circadian Clock-Regulated Gene Identified by Differential Displa
Immunohistochemistry of MMPs and TIMPs
Real-Time Analysis of Integrin-Dependent Transendothelial Migration and Integrin-Independent Interst
Three-Dimensional Human Tissue Models of Wounded Skin
Microscopic Computed Tomography-Based Virtual Histology of Embryos
Intracellular Staining Protocol
Transgenic Mouse Models of Craniofacial Disorders
Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice
High Proliferative Potential Colony Forming Cells
A Human Cell Extract-Based Assay for the Activation of ATM and ATR Checkpoint Kinases
Organ Cultures and Kidney-Capsule Grafting of Tooth Germs
The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells
Liposomes for Drug Delivery to Mitochondria
Demonstration of Mycoplasma Contamination in Cell Cultures by a Mycoplasma Group- Specific Polymeras
关卡(check point)
Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yea
Clonal Analysis in the Examination of Gene Function in Drosophila
Microencapsulation of Stem Cells to Study Cellular Interactions
Chemotaxis and Chemokinesis in 3D Macromolecular Matrices: Relevance to Angiogenesis
Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells
Real-Time Analysis of G Protein-Coupled Receptor Signaling in Live Cells
Measuring Apoptosis in Neural Stem Cells
Treatment of Rat Bladder Cancer With Electrochemotherapy In Vivo
The Post-embedding Method for Immunoelectron Microscopy of Mammalian Tissues: A Standardized Procedu
Tracking Inflammation-Induced Mobilization of Mesenchymal Stem Cells
Purification and Culture of Fetal Mouse Hepatoblasts that Are Precursors of Mature Hepatocytes and B
细胞融合(Cell fusion)实验
Development of Biological Tools to Study Claudins in the Male Reproductive Tract
In Vitro Selection of glmS Ribozymes
Activation of Rayon/Polyester Cloth for Protein Immobilization
Overview: Studying Integrins In Vivo
Immobilization of Proteins on Thionyl Chloride-Activated Controlled-Pore Glass
Quantification of Asymmetric Microtubule Nucleation at Subcellular Structures
bcl-2 原癌基因（bcl-2 protooncogene）
Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy
Adhesion to Matrix Proteins
选择蛋白(selectins)
Recording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration
Testing Smell When It Is Really Vital: Behavioral Assays of Social Odors in the Neonatal Mouse
Analysis of Endothelial Cell Migration Under Flow
Detection of c-Myc ProteinProtein Interactions and Phosphorylation Status by Immunoprecipitation
Culture of Human Endothelial Cells from Umbilical Veins
Genetic Analysis of Cystic Fibrosis Airway Epithelial Cells
Sphingolipids in Macroautophagy
A Genetic Model to Dissect the Role of Tsc-mTORC1 in Neuronal Cultures
Radionuclide-Peptide Nucleic Acid in Diagnosis and Treatment of Pancreatic Cancer
用尼龙毛法分离B细胞
Biophysical Methods to Probe Claudin-Mediated Adhesion at the Cellular and Molecular Level
Regulation of Transcription Factor Function by Targeted Protein Degradation: An Overview Focusing on
Expression of Recombinant MMP-28 in Mammalian Cells
Bioinformatic Identification of Plant Peptides
Epidermal Cell Analysis by RT-PCR
Assessing Mitochondrial Potential, Calcium, and Redox State in Isolated Mammalian Cells Using Confoc
Current Thoughts on the Therapeutic Potential of Stem Cell
Murine Nonvolatile Pheromones: Isolation of Exocrine-Gland Secreting Peptide 1
Determination of Hydroxyurea in Serum or Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS)
Generation of Induced Pluripotent Stem Cells with CytoTune, a Non-Integrating Sendai Virus
Studying Fertilization in Cell-Free Extracts: Focusing on Membrane/Lipid Raft Functions and Proteomi
Cryopreservation of Mammalian Embryos: Slow Cooling
Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cell
Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pe
Minimization of Proteins by Random Fragmentation and Selection
Cytological Analysis of Meiosis in Fixed Drosophila Ovaries
Isolation and Purification of Plant Nucleic Acids: Genomic and Chloroplast DNA
Hoechst 33342/PI双染色法
体内肝损伤模型（酒精）
293T细胞的一点培养心得
Adrenal chromaffin granule (chromaffin vesicle) preparation
人(ASD)酶联免疫分析
Lacrimal Experimental Protocol (4/4/94)
PCR of the Ribosomal DNA Intergenic Spacer Regions as a Method for Identifying Mosquitoes in the Ano
Enrichment of PBMCs with monocytes (The Science Advisory Board)
胚胎干细胞培养标准化操作规程
Production of Neurospheres from CNS Tissue
Isopycnic Centrifugation of DNA: Extraction and Fractionation of Melon Satellite DNA by Buoyant Dens
Immobilization of Enzymes on Ethynyl-Modified Electrodes via Click Chemistry
The Role of Redox in Signal Transduction
Overview of Missing Data Techniques
Maintenance of Chicken Embryonic Stem Cells In Vitro
Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes
Human Tracheal Gland Cells in Primary Culture
Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells
Culture Systems: Air Quality
Overview: Structural Biology of Integrins
Renal Gene Therapy
Analyzing Association of the Endoplasmic Reticulum with the Legionella pneumophilaContaining Vacuole
Enzyme Immobilization Using Chitosan-Xanthan Complexes
Isolation and Expansion of Mesenchymal Stem Cells/Multipotential Stromal Cells from Human Bone Marro
Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli
Histone Deacetylase Activity Assay
Genetic Manipulation to Analyze Pheromone Responses: Knockouts of Multiple Receptor Genes
Measuring Consequences of Protein Misfolding and Cellular Stress Using OMICS Techniques
Multiple Locus Variable Number of Tandem Repeats Analysis
供体
Single-Cell Microinjection Technologies
Pheromone Halo Assay
Functional Liposomal Membranes for Triggered Release
Functional Liposomal Membranes for Triggered Release
Immobilization of Candida rugosa Lipase on Superparamagnetic Fe3O4 Nanoparticles for Biocatalysis in
Isolation and Characterization of Human Hair Follicle Epithelial Cells
DNA and RNA Ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3)
Immunofluorescence Analysis of Human Spermatocytes
Evaluating Spindle Assembly Checkpoint Competence in Mouse Oocytes Using Immunoblotting
Measurement of GFP Expression and DNA Content in Permeabilized Cells
Ex Utero Culture and Live Imaging of Mouse Embryos
Affinity Chromatography: History, Perspectives, Limitations and Prospects
Rho GTPases in Hematopoietic Stem/Progenitor Cell Migration
Analysis of Chromatin Structure at Meiotic DSB Sites in Yeasts
Two-Color In Situ Hybridization of Whole-Mount Mouse Embryos
Bone Marrow-Derived Endothelial Progenitor Cells: Isolation and Characterization for Myocardial Repa
关于RPMI1640细胞培养液的配制
Isolation of Human Male Germ-Line Stem Cells Using Enzymatic Digestion and Magnetic-Activated Cell S
细胞运输(cellular transport)
Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells
Analysis of Oligonucleotides Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry
Imaging mRNAs in Living Mammalian Cells
Quantitative Analysis of Somatic Mitochondrial DNA Mutations by Single-Cell Single-Molecule PCR
Role of Natural Killer Cells and Killer Immunoglobulin-Like Receptor Polymorphisms: Association of H
Large Scale Purification of Brain Tubulin With the Modified Weisenberg Procedure
How to perform automated counts of fluorescently stained cells.
Practical Considerations of Membrane Protein Instability during Purification and Crystallisation
Arginine and Macrophage Activation
High-Yield Isolation and Subcellular Proteomic Characterization of Nuclear and Subnuclear Structures
The Identification of Dengue Virus Using PCR
Neural Differentiation of Human Adipose Tissue-Derived Stem Cells
个别组织细胞的培养
Phylogenomic Resources at the UCSC Genome Browser
Cellular Populations Isolated from Newborn Mouse Skin Including Mesenchymal Stem Cells
Automated Pattern Ranking in Differential Display Data Analysis
Human Mononuclear Phagocytes in Tissue Culture
Spatiotemporal Regulation of Ras-GTPases During Chemotaxis
Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells
Pax3 and Vertebrate Development
PNA-FISH Technique for In Situ Assessment of Aneuploidy
Screening and Selection Strategies for Disulfide Isomerase Activity
Relative Reverse Transcription-Polymerase Chain Reaction
Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis
Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media
膜结合蛋白(membrane-binding proteins)
Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling
Isolation and Purification of Murine Male Germ Cells
Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential
ATP-Loaded Liposomes for Targeted Treatment in Models of Myocardial Ischemia
Designing RNAi Screens to Identify JAK/STAT Pathway Components
Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems
Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology
Confrontation Cultures of Embryonic Stem Cells With Multicellular Tumor Spheroids to Study Tumor-Ind
Phylogenomic Analysis by Chromosome Sorting and Painting
Inducible Expression in Human Cells, Purification, and In Vitro Assays for the Mitochondrial DNA Hel
High Cell-Density Fermentation
Locomotor Activity in Rodents
Chemical Induction of Apoptosis
Human Embryonic Stem (ES) Cell Protocols――Thawing Human ES cells
Co-IP免疫共沉淀实操注意事项
Co-IP免疫共沉淀实验常见问题及解决方法
Northern Blot印迹杂交实验介绍
Northern Blot印迹杂交实验详细操作步骤
Southern Blot印迹杂交实验介绍
Southern Blot印迹杂交实验操作步骤
Southern blot印迹杂交实验常见问题与注意事项
EMSA凝胶迁移实验原理介绍
EMSA实验详细操作步骤
EMSA凝胶迁移实验常见问题与注意事项
石蜡切片实验介绍
石蜡切片制作步骤
石蜡切片制作过程中的注意事项
石蜡切片制作常见问题与解决方法【上】
石蜡切片制作常见问题与解决方法【下】
Microarray Data Analysis in Studies of Membrane Transporters
Gene Expression Profiling of Mouse Epidermal Keratinocytes
Stable Integration of Vectors at High Copy Number for High-Level Expression in Animal Cells
Solid Phase Assays for Studying ECM Protein-Protein Interactions
Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy
Cryoelectron Microscopy of Vitreous Sections: A Step Further Towards the Native State
Exploiting the Liberation of Zn2+ to Measure Cell Viability
将构建的真核表达载体转染进HEK293细胞的方法
Molecular Cytogenetic Applications in Diagnostics and Research: An Overview
Differentiation of Dendritic Cells from Human Embryonic Stem Cells
DNA甲基化实验介绍
The Application of Differential Display to the Brain: Adaptations for the Study of Heterogeneous Tis
DNA甲基化实验操作步骤
Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Built-In Pillar Array
Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes
六种甲基化检测方法
Fluorescent Differential Display
研究新热点之细胞焦亡
Analysis of Janus Tyrosine Kinase Phosphorylation and Activation
细胞焦亡的研究方法与检测方式
EMS Screens: From Mutagenesis to Screening and Mapping
Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells
Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos
细胞焦亡在临床各领域的研究现状
In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET
Quantitative Fluorescence In Situ Hybridization on Paraffin Embedded Tissue
Fluorescence Immunohistochemistry in Combination with Differential Interference Contrast Microscopy
Exploring the Link Between Human Embryonic Stem Cell Organization and Fate Using Tension-Calibrated
白色体(etioplast)
原位杂交实验
Difficult Proteins
Immunocytochemistry on Suprachiasmatic Nucleus Slices
组织FISH实验操作步骤
Methods for Data Analysis
荧光原位杂交实验操作步骤
Micellar Electrokinetic Chromatography of Aminoglycosides
Computational Modeling of Signal Transduction Networks: A Pedagogical Exposition
常用细胞凋亡检测方法（五）
Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology
Identification and Quantitation of Cocaine, Benzoylecgonine, and Cocaethylene in Blood, Serum, and P
Rap1 and Integrin Inside-Out Signaling
Fluorometric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis
High-Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Media
Aging of Cultured Human Skin Fibroblasts
原位杂交实验操作注意事项
Gametic Cell Adhesion and Fusion in the Unicellular Alga Chlamydomonas
原位杂交实验操作常见问题答疑
Fusion and Cloning
裸鼠皮下成瘤实验步骤与介绍
Prokaryotic Phototaxis
裸鼠皮下成瘤实验操作注意事项
Analysis of mRNA Expression During Preimplantation Development
裸鼠皮下成瘤实验常见问题处理方法
Histological Processing of Teeth and Periodontal Tissues for Light Microscopy Analysis
冰冻切片实验介绍
Infection with retroviruses
冰冻切片详细操作步骤
General Linear Models
Chromatin Immunoprecipitation Technique for Study of Transcriptional Dysregulation in Intact Mouse B
冰冻切片操作注意事项
High-Throughput Screen for Aromatic Hydroxylation
Evaluation of Circulating Endothelial Precursor Cells in Cancer Patients
冰冻切片制片的影响因素
冰冻切片与石蜡切片的区别
酵母遗传学方法11：PCR介导基因破坏法
什么是HE染色法？
联会(synapsis)
Promoter Analysis in Zebrafish Embryos
HE染色实操注意事项
In Situ Hybridization to Plant Tissues and Chromosomes
Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro
HE染色常见问题与处理方法
HE染色实验问题答疑
免疫组化实验技术介绍【
免疫组化/IHC实验操作步骤
免疫组化/IHC实验操作注意事项
免疫组化（IHC）染色常见问题及其解决方法上
免疫组化/IHC染色常见问题及其解决方法下
免疫组化和免疫荧光有什么区别？
什么是免疫荧光技术？
细胞/组织免疫荧光实验操作步骤
免疫荧光实操注意事项汇总
免疫荧光常见问题及其解决方法
什么是细胞增殖？
细胞增殖检测方法汇总
Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Nativ
Isolation and Characterization of Cancer Stem Cells In Vitro
Cell-Based Co-transfection Microarrays for Use with HEK293T Cells on a Poly d-Lysine-Coated Polystyr
Mosaic Analysis in Caenorhabditis elegans
Fine Structure of the Autophagosome
Antisense Therapeutics in the Central Nervous System: The Induction of c-fos
Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Subtrates
Determination of Gene Promoter Activity in Skeletal Muscles In Vivo
Single Molecule Tracking for Studying Nucleocytoplasmic Transport and Intranuclear Dynamics
Application of Differential Display in Studying Developmental Processes
Preparation of RNA from Cultured Cells
Fertilization and Sperm Chemotaxis in Ascidians
Derivation of High-Purity Oligodendroglial Progenitors
A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA
Growth and Differentiation of Human Adipose Stromal Cells in Culture
旁分泌细胞（paracrine cell）
Primary Cell Cultures for the Study of Myelination
Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents
Bioluminescence: Imaging Modality for In Vitro and In Vivo Gene Expression
High-Yield Purification of Functionally Active Glucocorticoid Receptor
Genetically-Encoded Fluorescent Probes for Imaging Endogenous mRNA in Living Cells
Gene Therapy Using Neural Stem Cells
Inducible Gene Expression in Transient Transgenic Xenopus Embryos
肌质网(sarcoplasmic reticulum)
猪白细胞介素-2（IL-2）酶联免疫分析（ELISA）
Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes
Studying G2 DNA Damage Checkpoints Using the Fission Yeast Schizosaccharomyces pombe
In Vitro Assays for Tumors Grown In Vivo: A Review of Kinetic Techniques
Intracellular ATP Delivery Using Highly Fusogenic Liposomes
Fluorescence Imaging of Mitochondria in Yeast
Characterizing the Phenotype of Murine Epidermal Progenitor Cells: Complementary Whole-Mount Visuali
Conditional Gene Knockout Using Cre Recombinase
Assays of Lipogenic Enzymes
Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors
Video-EEG Monitoring Methods for Characterizing Rodent Models of Tuberous Sclerosis and Epilepsy
细胞复苏后贴壁细胞较少的问题分析
LPS介导的MAPK信号通路
专为转染HEK 293细胞而设计的转染试剂盒
MAPK级联信号通路图
IP3受体(IP3 receptor)
High Resolution PCR Quantitation by AmpliSensor Assay
Analysis of Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis
In Situ Zymography
An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1
Reversion of Mouse Postimplantation Epiblast Stem Cells to a Nave Pluripotent State by Modulation of
Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases
Detection and Characterization of Leishmania Parasites by DNA-Based Methods: Southern Blotting and P
Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay
氧还电位(oxidation-reduction potentials, redox potentials)
Assessing Bad Sub-cellular Localization Under Conditions Associated with Prevention or Promotion of
Oocytes Microinjection Assay to Study the MAP-Kinase Cascade
细胞复苏之我见－经验总结
几种细胞培养基M199中维生素含量的测定方法
植物多倍体诱发实验
“黑胶虫”问题
Onco-CYTE(TM):不需要FBS的细胞培养基
核小体(nucleosome)
大鼠原代肾小管上皮细胞培养方法
关于细胞培养中支原体污染的经验分享
Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein
细胞核与线粒体的分级分离
Matrigel invasion assays
Quantitation of Iothalamate in Urine and Plasma Using Liquid Chromatography Electrospray Tandem Mass
Metaphase chromosome preparation
Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Act
间隔基因(gap genes)
造血干细胞(hematopoietic stem cell，HSC)
受体钝化(receptor desensitization)
Tunel Procedure in Bovine Embryos 牛胚胎TUNEL检测凋亡
光系统Ⅰ复合物(photosystem Ⅰcomplex, PSⅠcomplex)
ELISA实验中血清标本的采集、保存及注意事项
Transcriptional Regulatory Mechanisms That Govern Embryonic Stem Cell Fate
Polyethylene Glycol-Mediated Cell Fusion
染色体组型分析
Comparative Purification Strategies for Drosophila and Human Mitochondrial DNA Replication Proteins:
红霉素(Erythromycin)酶联免疫分析（ELISA）
Selection of Transfected Suspension Cells
外核膜(outer nuclear membrane)
细胞培养基中的添加剂及其作用
人类人工染色体的构建策略
Apoptosis TUNEL Assay (frozen sections)
奢侈基因(luxury gene)
Membrane Protein Solubilization
Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells
A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Respo
Matrigel invasion assays
Microscale Thermophoresis for the Assessment of Nuclear Protein-Binding Affinities
Cryopreservation of Human Red Blood Cells
A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays
Immobilization of Enzymes on Glyoxyl Agarose: Strategies for Enzyme Stabilization by Multipoint Atta
Bovine Somatic Cell Nuclear Transfer
Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells
Migration Assays for Oligodendrocyte Precursor Cells
self-assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses
Localization of rDNA at Nucleolar Structural Components by Immunoelectron Microscopy
Isolation and Analyses of Enriched Populations of Male Mouse Germ Cells by Sedimentation Velocity: T
Plant Mitochondrial Transcriptomics by Quantitative RT-PCR
Isolation of Mesenchymal Stem Cells from Murine Bone Marrow by Immunodepletion
Solid Tumor Cancer Markers and Applications to Steroid Hormone Research
Specific PCR Primers for the Identification of Endomycorrhizal Fungi
Combined Total Proteomic and Phosphoproteomic Analysis of Human Pluripotent Stem Cells
Forkhead (FOX) Transcription Factors and the Cell Cycle: Measurement of DNA Binding by FoxO and FoxM
Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Rec
Full Sequencing of Viral Genomes: Practical Strategies Used for the Amplification and Characterizati
Culture of Megakaryocytes and Platelets from Subcutaneous Adipose Tissue and a Preadipocyte Cell Lin
CELL CYCLE ANALYSIS
核内不均一RNA
接触抑制(contact inhibition)
Identification of Musashi1-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by
Exploring RNA Virus Replication in Xenopus Oocytes
Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery
In Situ Hybridization to Polytene Chromosomes of Drosophila melanogasterand Other Dipteran Species
Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins
Assessing In Vivo MicroRNA Function in the Germline Stem Cells of the Drosophila Ovary
Analysis of Gene Expression in Skin Using Laser Capture Microdissection
Fluorescence In Situ Hybridization for Molecular Cytogenetic Analysis in Filamentous Fungi
Immunofluorescence Staining of Spindles, Chromosomes, and Kinetochores in Human Oocytes
Free-Hand Bisection of Mouse Oocytes and Embryos
SOLID TUMOR CULTURES FOR CHROMOSOME ANALYSIS
Measurement of Apoptotic Cell Clearance In Vitro
Adenoviral Transduction of Mesenchymal Stem Cells
Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells
Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies: Real-Time Detection
Essentials of Recombinase-Based Genetic Fate Mapping in Mice
Assessing Chronological Aging in Bacteria
Cell-Adhesion Assays: Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Mem
Analysis of Lipoplex Structure and Lipid Phase Changes
In Situ Hybridization Using Riboprobes on Free-Floating Brain Sections
Tyramide Signal Amplification for DNA and mRNA In Situ Hybridization
Induction of Deoxyribonucleic Acid Damage by Irradiation
Messenger RNA Turnover in Cell-Free Extracts from Higher Eukaryotes
Penetratin Story: An Overview
Gene Silencing Using RNA Interference in Embryonic Stem Cells
Bisulfite Treatment of DNA
无血清培养基及其使用方法概述
细胞培养设施和基本条件
Nuclear Receptors: One Big Family
Tissue Engineering and Cell-Populated Collagen Matrices
Monitoring RNALigand Interactions Using Isothermal Titration Calorimetry
Use of Peptide Libraries to Determine Optimal Substrates of Tyrosine Kinases
Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondri
Purification of Human PARP-1 and PARP-1 Domains from Escherichia coli for Structural and Biochemical
Thermodynamics of Lipid Interactions with Cell-Penetrating Peptides
Whole Body MRI and Fluorescent Microscopy for Detection of Stem Cells Labeled with Superparamagnetic
In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method
Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Mu
Treatment of Liver Tumors in Rabbit
Analysis of Chromosome Dynamics and Chromosomal Proteins in Drosophila Spermatocytes
Imaging of Schwann Cells In Vivo
Transplantation of Chimeric Fetal Liver to Study Hematopoiesis
Immobilization of Enzymes Acting on Macromolecular Substrates: Reduction of Steric Problems
Lentiviral Vector-Mediated Gene Transfer and RNA Silencing Technology in Neuronal Dysfunctions
Measuring Ca2+ Oscillations in Mammalian Eggs
Transition Metal Methods for Immobilization of Enzymes and Cells
Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Tech
Cell Fusion in the Filamentous Fungus, Neurospora crassa
Collagen Gel Assay for Angiogenesis: Induction of Endothelial Cell Sprouting
Mesenchymal Stem Cells and Nano-structured Surfaces
Noninvasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs
Tissue Culture Correlational Study of Genetic Cholangiopathy of Autosomal Recessive Polycystic Kidne
From Gene-Scale to Genome-Scale Phylogenetics: the Data Flood In, but the Challenges Remain
Study Design: The Basics
Mouse Models of Triplet Repeat Diseases
Detection of Cytoplasmic and Nuclear Functions of mTOR by Fractionation
Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis
Live Imaging for Studying Asymmetric Cell Division in the C. elegans Embryo
Organotypic raft culture of primary human keratinocytes (PHKs)
Application of Plastic Embedding for Sectioning Whole-Mount Immunostained Early Vertebrate Embryos
Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization
Analysis of Cell Death by Electron Microscopy
Imaging of Embryonic Stem Cell Migration In Vivo
非内共生学说
流式细胞术:标本处理
PI3K-Akt信号通路的抑制位点
Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader
Hematopoietic and Nature Killer Cell Development from Human Pluripotent Stem Cells
Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes
粘着带(adhesion belt)
Entrapment in Calcium Alginate
大鼠成纤维细胞生长因子（FGF）酶联免疫分析
Genetic Complementation Protocols
An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics
Radioligand-Binding Methods for Membrane Preparations and Intact Cells
Quantifying Amplicons with ELISA
Isolation of Microtubules (Bovine Brain)
Screening for Thermostability
外周血培养基的配制
cck8法检测细胞增殖详细操作步骤
细胞增殖检测之MTT法
EDU法检测细胞增殖操作
细胞增殖检测的注意事项
什么是彗星实验？
彗星实验/单细胞凝胶电泳实验操作步骤
彗星实验/单细胞凝胶电泳实验注意事项
彗星实验操作常见问题答疑
细胞衰老的介绍与研究意义是什么？
细胞衰老的检测方法
贴壁细胞、悬浮细胞、组织切片衰老实验操作步骤
什么是实时荧光定量PCR？
荧光定量PCR实验操作步骤
荧光定量PCR结果分析解读
荧光定量PCR实操注意事项
实时荧光定量PCR常见问题及解决方法
实时荧光定量PCR实验技术答疑
什么是SNP/单核苷酸多态性？
SNP/单核苷酸多态性检测最常用的三种方法
SNP检测方法总结
Taqman探针法检测SNP分型详解
直接测序法检测SNP详解——最常用的方法
SNP检测常见问题答疑上篇
SNP检测常见问题答疑下篇
什么是血管生成实验？
血管生成实验操作步骤
什么是细胞培养？
动物细胞原代培养操作步骤
动物细胞传代培养详细步骤
植物组织的培养方法
植物细胞的培养方法总结
细胞培养/细胞实验操作注意事项
细胞培养实常见问题及处理汇总
细胞冻存与复苏的操作步骤
细胞冷冻与复苏操作注意事项
细胞冻存与复苏问题汇总大全
细胞培养基的理化性质和基本成分
细胞操作的无菌环境与操作要点
什么是细胞划痕实验？
细胞划痕实验操作详细步骤
细胞划痕实验操作注意事项
细胞划痕实验操作问题答疑
Tunel染色实验原理与步骤详解
Tunel染色实操注意事项
Tunel染色实验的常见问题与解决方法
什么是酵母双杂系统？
酵母双杂详细操作步骤
酵母双杂实验注意事项
酵母双杂交常见问题及解决方法上
酵母双杂交常见问题及解决方法下
Optical Imaging of Cell Fusion and Fusion Proteins in C aenorhabditis elegans
Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short H
Rat Osteoblast Cultures
Electron Microscopy and In Situ Hybridization: Expression of P2Y2 Receptor mRNA in the Cerebellum
Embryological Methods in Ascidians: The Villefranche-sur-Mer Protocols
半桥粒(hemidesmosomes)
Endogenous Biotin in Rat Brain: Implications for False-Positive Results With AvidinBiotin and Strept
Studying the Structure of Microtubules by Electron Microscopy
Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNA-Lipid Complexes
RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 cells
Monitoring Integrin Activation by Fluorescence Resonance Energy Transfer
光呼吸(photorespiration)
CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors
人脱氧吡啶啉（DPD）酶联免疫分析（ELISA）
In Vitro Derivation and Expansion of Endothelial Cells From Embryonic Stem Cells
Mammalian Cell Microinjection Assay to Study the Function of Rho Family Guanosine Triphosphatases
Assaying Cytochrome c Translocation During Apoptosis
Differential Screening of Differential Display cDNA Products by Reverse Northern
Imaging B-Cell Receptor Signaling by Single-Molecule Techniques
Revised protocol for establishing a mammary cell line from tumors
Analysis of Chaperone Properties of Small Hsp
Ligand Binding and Processing: The Perfused Liver as a Model System
Improving Lipase Activity by Immobilization and Post-immobilization Strategies
Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard
核周腔(perinuclear space)
Double Indirect-Immunofluorescent Labeling of Cultured Cells
Analysis of Lipid Profiles in Skeletal Muscles
Quantification of Genomic Mutations in Murine Hematopoietic Cells
Indirect Immunofluorescence for Monitoring Spindle Assembly and Disassembly in Yeast
Identification of Cryptic Sex Chromosomes and Isolation of X- and Y-Borne Genes
Studies of Proteoglycan Involvement in CPP-Mediated Delivery
Capillary Electrophoretic Analysis of Organic Pollutants
The Use of Embryonic Stem Cells to Study Hedgehog Signaling
Cell Migration: An Overview
Germline Clone Analysis for Maternally Acting Drosophila Hedgehog Components
Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System T
Clustering Assay for Studying the Interaction of Membrane Proteins with PDZ Domain Proteins
Microtiter-Plate Assay and Related Assays for Nonspecific Endonucleases
Deriving Mouse Spermatogonial Stem Cell Lines
Immobilization of Cells Using Electrostatic Droplet Generation
In Vitro Electrophysiological Mapping of Stem Cells
Use of In Vivo Gap Repair for Isolation of Mutant Alleles of a Checkpoint Gene
BacMam-Mediated Gene Delivery into Multipotent Mesenchymal Stromal Cells
Laser Desorption Ionization Mass Spectrometry of Bioorganic Molecules
Mammalian Expression Systems and Transfection Techniques
Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry
Evaluation of Skull and Tooth Morphology and Mineralization Using High-Resolution X-Ray Tomography
Isolation of Common Dendritic Cell Progenitors (CDP) from Mouse Bone Marrow
Hollow Fiber Assay for Tumor Angiogenesis
大规模培养常用方法
Formation of Gut-Like Structures In Vitro From Mouse Embryonic Stem Cells
动物染色体标本的制备
Affinity Chromatography of Phosphorylated Proteins
Immobilization of Enzymes by Selective Adsorption on Biotinylaminopropyl Celite or Glass
Keratinocyte Culture in the Absence of Substrate Attachment
Isolation and Propagation of Colon Cancer Stem Cells
Extracorporeal Assays of Thrombosis
Live Cell Fluorescence Microscopy Techniques
FRAP Analysis of Chemosensory Components of Dictyostelium
Multiplex Gene Expression Analysis by TRAC in Fungal Cultures
Angiogenic Vessel-Targeting DDS by Liposomalized Oligopeptides
Palate Development: In Vitro Procedures
ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy
Evaluation of VEGF-Induced Vascular Permeability in Mice
Comparative Pharmacokinetics of Antisense Oligonucleotides
Health and Neonatal Care of Bovine Clones
Oxidative Damage in Huntington’s Disease
cRNA Probes: Comparison of Isotopic and Nonisotopic Detection Methods
New Protocol for Lentiviral Vector Mass Production
Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation
The Genome of Dictyostelium discoideum
Methods for Assessing Effects of Wnt/-Catenin Signaling in Senescence of Mesenchymal Stem Cells
Imaging Cells Within 3D Cell-Derived Matrix
Purification of Proteins Using Displacement Chromatography
Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Site-Directed Mutagenesis to Study the StructureFunction Relationships of Ion Channels
Preparation of Immobilized Subunits of a Multisubunit Enzyme
MTT Cell Proliferation Assay
Gene Expression Analyzed by Ribonuclease Protection Assay
Methods for Gene Expression Profiling in Dermatology Research Using DermArray Nylon Filter DNA Micro
Serial Section Immunoelectron Microscopy of Algal Cells
Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants
中心粒(centrioles)
小鼠白细胞介素-6（IL-6）酶联免疫分析（ELISA）
Transfection of Mammalian Cells Using Lipofectamine
Redox Property of Ribonucleotide ReductaseSmall Subunit M2 and p53R2
Confocal Microscopy of Adipocytes
Using Intrinsic Fluorescence Emission Spectroscopy to Study Steroid Receptor and Coactivator Protein
细胞培养技术注意事项大汇总
MTT的升级替代产品——CCK-8
Histochemical Localization of Apoptosis With In Situ Labeling of Fragmented DNA
An Affinity Oligonucleotide Displacement Strategy to Purify Ribonucleoprotein Complexes Applied to H
Purification and Mass-Spectrometry Identification of Microtubule-Binding Proteins from Xenopus Egg E
细胞融合实验流程
细胞融合实验流程
Protein Import Into Isolated Mitochondria
In Vivo Optical Imaging of Ischemic BloodBrain Barrier Disruption
Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry
Assessment of Impaired Proteasomal Function in a Cellular Model of Polyglutamine Diseases
微染色体－微管结合实验（Minichromosome
Cell counting/plating
遗传学实验之——染色体组型分析
简单扩散(simple diffusion)
P-Element Mutagenesis
Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phos
Methods for the Assessment of Telomere Status
Utilization of Digital Differential Display to Identify Novel Targets of Oct3/4
Nucleotide Excision Repair Assay in Drosophila melanogaster Using Established Cell Lines
Culturing Human Schwann Cells
Chemokine Receptor Dimerization and Chemotaxis
Application of FISH to Archival Cytogenetic Slides
Interaction of Genetic and Environmental Factors in Saccharomyces cerevisiae Meiosis: The Devil is i
什么是酵母单杂实验？
酵母单杂交实验详细步骤
酵母杂交实验的常见问题—转化篇
酵母杂交实验的常见问题—试剂篇
什么是RNA pulldown实验？
RNA pull-down详细实验步骤
RNA pull-down注意事项与常见问题
RNA pull-down实验问题答疑
DNA pull-dwon实验介绍
DNA pulldown实验操作步骤
DNA pulldown实验问题答疑
GST pulldown实验原理与介绍
GST pulldown实验操作步骤
什么是耐药细胞株？
耐药细胞株构建详细步骤步骤
耐药细胞株构建注意事项
脓毒症动物模型介绍
脓毒症模型构建操作步骤
非酒精性脂肪肝造模原理与方法
非酒精性脂肪肝造模详细步骤
小鼠肠炎造模实验介绍
小鼠肠炎造模操作步骤
什么是糖尿病动物模型？
A Novel Role for Nutrition in the Alteration of Functional Microdomains on the Cell Surface
糖尿病动物模型造模步骤
Insect Pheromone Behavior: Fruit Fly
糖尿病动物造模注意事项
Methods of Telomerase Inhibition
肝纤维化动物造模机制介绍
Screening of Nanoparticle Embryotoxicity Using Embryonic Stem Cells
Reproductive and Developmental Manipulation of the Marsupial, the Tammar Wallaby Macropus eugenii
肝纤维化动物造模步骤上篇
Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry
Analytical Methods for Cancer Stem Cells
Assessing BloodBrain Barrier Function Using In Vitro Assays
Separation of Synthetic (Co)Polymers by Capillary Electrophoresis Techniques
Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR)
合成代谢（anabolism）
Cell Disruption and Subcellular Fractionation
Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye
Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis
Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions
肝纤维化动物造模步骤下篇
羊水细胞染色体标本的制备
Mimicry of Protein Function with Cell-Penetrating Peptides
关节炎动物造模原理与介绍
人褪黑素 (melatonin)酶联免疫分析
关节炎动物造模方法介绍
In Vitro Production of Enucleated Red Blood Cells from Hematopoietic Stem and Progenitor Cells
Cell-Cell Adhesive Interactions in an In Vitro Flow Chamber
关节炎动物模型造模步骤
Efficient Isothermal Amplification of the Entire Genome from Single Cells
脑缺血模型实验介绍
Analysis of Apoptosis by Flow Cytometry
脑缺血模型造模步骤—上
Method: Logging in Specimens and Record Keeping
Monitoring Aggregate Formation in Organotypic Slice Cultures From Transgenic Mice
一些干细胞标志物
Recording Dendritic Ion Channel Properties and Function from Cortical Neurons
Biological Membranes as Protein Aggregation Matrices and Targets of Amyloid Toxicity
Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Developm
Live-Cell Imaging of Microtubule Dynamics in Hyphae of Neurospora crassa
egakaryocyte and Platelet Production from Human Cord Blood Stem Cells
Assessment of Oxidative Stress in Sperm and Semen
Molecular Profiling of Mitochondrial Dysfunction in Caenorhabditis elegans
脑缺血模型造模步骤—下
脑缺血动物造模实操注意事项
活性氧（ROS）检测介绍
活性氧检测荧光法实验步骤
活性氧检测化学发光法实验步骤
什么是支原体检测？
支原体检测方法大全
PCR检测支原体操作步骤
培养法检测支原体操作步骤
DNA染色法检测支原体操作步骤
Dot blot斑点杂交实验原理与方法
RNA Dot Blot斑点杂交实操步骤
淋巴细胞亚群检测实验原理与介绍
Th淋巴细胞亚群检测步骤
什么是粒细胞？
粒细胞检测实验原理与流程
什么巨噬细胞？
Isolation, Characterization, and Culture of Normal Human Intestinal Crypt and Villus Cells
Phosphoinositides, Inositol Phosphates, and Phospholipase C in Embryonic Stem Cells
Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cas
Molecular Cytogenetics: The Standard FISH and PRINS Procedure
Molecular Links Between Autophagy and Apoptosis
Live Imaging of Primitive Endoderm Precursors in the Mouse Blastocyst
Identification of Novel Genes Involved in Adipose Differentiation by Differential Display
Quantitation of Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine Using Gas Chromatog
Association Methods in Human Genetics
Analysis of LINE-1 Expression in Human Pluripotent Cells
小鼠白细胞介素-10（IL-10）酶联免疫分析（ELISA）
Characterization of Constitutively Active Mutants of G Protein-Coupled Receptors
The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components
重复序列(repetitive sequence)
Cell-Specific Aptamers for Targeted Therapies
Adenovirus General Protocol for Infecting Cells
牙周膜细胞和牙髓细胞的培养方法
Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods
Salt-Urea, Sulfopropyl-Sepharose, and Covalent Chromatography Methods for Histone Isolation and Frac
Cell Surface Engineering of Mesenchymal Stem Cells
伪装的mRNA（masked mRNA）
Measuring Programmed Cell Death in Plants
细胞因子信号网络图(5张)
FISH for Mapping Single Copy Genes
Ultrastructural and Immunofluorescent Methods for the Study of the XY Body as a Biomarker
Embryonic Stem Cells as a Model for Studying Melanocyte Development
微丝(microfilament)
Fusion Assays and Models for the Trophoblast
Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Ta
Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal
Separation of Peptides by Capillary Electrophoresis
骨髓细胞染色体标本制备
Yeast Mitochondrial Transcriptomics
Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins
Lentivirus-Based Virus-Like Particles as a New Protein Delivery Tool
Cell Membrane Vesicles as a Tool for the Study of Direct EpithelialStromal Interaction: Lessons from
Fluorescence-Based Tl+Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca
A Method to Isolate and Culture Expand Human Dental Pulp Stem Cells
Cell Culture of Primary Cerebellar Granule Cells
Isolation of Primary Avian Osteocytes
Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cells
Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs
Expression of Tetanus Toxin Fragment C
In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors
Electrical Recordings from the Accessory Olfactory Bulb in VNO-AOB Ex Vivo Preparations
Making cRNA for Microinjection and Expression of Fluorescently Tagged Proteins for Live-Cell Imaging
Ex Vivo Stromal Cell Electroporation of Factor IX cDNA for Treatment of Hemophilia B
Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Bra
Methods for the Study of Entosis
Real-Time Spectrofluorometric Assays for the Lumenal Environment of the Maturing Phagosome
Culture Systems: Sequential
Identification of Target Membrane Proteins as Detected by Phage Antibodies
Nitrocellulose Filter Binding for Determination of Dissociation Constants
Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/A
Detection of Chromatin-Bound PCNA in Cultured Cells Following Exposure to DNA-Damaging Agents
Ex Vivo Organ Culture of Human Hair Follicles: A Model EpithelialNeuroectodermalMesenchymal Interact
Sequencing of Viral Genes
Methods to Determine the Status of Mitochondrial ATP Synthase Assembly
Measurement of Adipose Tissue Blood Flow
大鼠胆固醇（CH）酶联免疫分析
SpermEgg Fusion Assay in Mammals
Preparation, Characterization, and Use of Antioxidant-Liposomes
Genetic Modification of Chondrocytes Using Viral Vectors
三联管(triplet)
Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut
Principles for Derivation of Human Embryonic Stem Cells
Protocols for Studying Antizyme Expression and Function
The efficient generation of mesenchymal cells such as adipocytes, osteoblasts, and chondrocytes from
Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells
Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium
In Vitro Human T Cell Development Directed by NotchLigand Interactions
干细胞(stem cells, SC)
Detecting Expression Patterns of Wnt Pathway Components in Nematostella vectensis Embryos
巨噬细胞培养方法与步骤
巨噬细胞分离与纯化步骤
巨噬细胞极化实验步骤
巨噬细胞流式检测方法
什么是亚硫酸氢钠测序？
亚硫酸氢钠测序（BSP）实验步骤
单细胞悬液制备方法—下
什么是组织芯片？
组织芯片详细制作步骤
组织芯片制作注意事项
甲醇刚果红染色实验介绍
刚果红染色实验步骤
动物组织样本DNA提取步骤
植物组织样本DNA提取步骤
考马斯亮蓝染色法实验介绍
TTC染色实验介绍
TTC染色实验步骤
什么是番红固绿染色？
动物组织番红固绿染色步骤
植物组织番红固绿染色步骤
什么是RIP检测？
RIP检测实验操作步骤
RIP检测样本制备注意事项
动物组织甲苯胺蓝染色步骤
什么是外泌体？
外泌体提取步骤
外泌体鉴定的三个标志与应用
组织切片脱水后不能及时包埋怎么办？
细胞划痕实验最佳设置时间是多久？
taqman探针两段带有什么基因？
实验用的植物组织蛋白样本怎样提取出来？
肿瘤组织怎样提取蛋白？
组织做冰冻切片一般是什么温度比较合适？
做石蜡切片时石蜡带弯曲不直怎么办？
什么是免疫荧光的半定量分析？
石蜡切片碎掉的蜡块可以重新包埋吗？
做石蜡切片时组织总是碎裂怎么办？如何避免？
石蜡切片总是厚薄不均匀怎么办？
石蜡切片染色不均匀模糊怎么办？
什么是单细胞悬液？有什么用？
小鼠成瘤不理想可以多次接种吗？
什么是荧光定量pcr三步法？
QPCR中的CT值是什么意思？
QPCR荧光定量基因表达量太低跑不出来怎么办？
冰冻切片染色模糊不清什么原因？
双荧光素酶实验细胞一般转染多久？
免疫荧光染不上色怎么办？
彗星实验结果怎么分析？
双荧光素酶的两个报告基因载体作共转染应用什么条件呢？
裸鼠成瘤肿瘤为什么长起来又消失了？
High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR
CDK-Activating Kinases: Detection and Activity Measurements
Visualization of mRNA Localization in Xenopus Oocytes
常规组织初代消化培养
The Human Embryo: Ethical and Legal Aspects
MT Spindowns from Extracts
Identification and Quantitation of G-Protein α-Subunits
In Vivo Measurement of Glioma-Induced Vascular Permeability
细胞培养常见问题、可能原因及解决方法
Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extre
Traditional Human Embryonic Stem Cell Culture
Isolation of the Constitutive Heterochromatin from Mouse Liver Nuclei
Single-Channel Analysis of TRPC Channels in the Podocytes of Freshly Isolated Glomeruli
基因疫苗 Gene Vaccine
次级溶酶体(secondary lysosome)
Neural Stem Sphere Method: Induction of Neural Stem Cells and Neurons by Astrocyte-Derived Factors i
Visualisation of RNA by Electron Microscopic In Situ Hybridisation
顶体(acrosome)
Periodic Activation of ERK2 and Partial Involvement of G Protein in ERK2 Activation by cAMP in Dicty
Neoepitope Antibodies Against MMP-Cleaved and Aggrecanase-Cleaved Aggrecan
Simultaneous DNA/RNA Analysis of Solid and Hematoreticular Malignancies
Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports
人角化细胞生长因子(KGF)酶联免疫分析（ELISA）
Archaebacterial Tetraetherlipid Liposomes
Extraction and Immunoblotting of Proteins From Embryos
Isolation of Muscle Stem Cells by Fluorescence Activated Cell Sorting Cytometry
Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronect
Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayers
Analysis of the Cell Cycle in Mouse Embryonic Stem Cells
Basic Bayesian Methods
The Macrophage
In Vitro Construction of 2D and 3D Simulations of the Murine Hematopoietic Niche
Basic Cell Culture
Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cell
Solubilization of Proteins in 2DE: An Outline
Recording of Ion Channel Activity in Planar Lipid Bilayer Experiments
The Use of Degenerate Primers in Conjunction with Strain and Species Oligonucleotides to Classify On
Cripto Signaling in Differentiating Embryonic Stem Cells
Site-Directed Mutagenesis Using Overlap Extension PCR
Identification and Isolation of Rodent Respiratory Tract Dendritic Cells
Determination of DNA Mutation Load in Human Tissues Using Denaturing HPLC-Based Heteroduplex Analysi
The Xenopus Embryo as a Model System for Studies of Cell Migration
Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs)
In Vivo Measurement of Muscle Protein Synthesis Rate Using the Flooding Dose Technique
Paraformaldehyde Fixation of Cells
Cuticle Preparation of Drosophila Embryos and Larvae
NF-kappaB信号通路（10张）
Clinical Uses of Liver Stem Cells
Proteomic Tools for the Analysis of Cytoskeleton Proteins
A Tutorial on Mathematical Modeling of Biological Signaling Pathways
带蛋白(band protein)
Two-Dimensional Separation of Membrane Proteins by 16-BAC-SDS-PAGE
Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies
Identification of Disease Markers by Differential Display: Prion Disease
LC-MS/MS Analysis of 13 Benzodiazepines and Metabolites in Urine, Serum, Plasma, and Meconium
肌球蛋白(myosin)
Live-Cell Imaging of the Cytoskeleton and MitochondrialCytoskeletal Interactions in Budding Yeast
Analysis of Microtubule Dynamics by Polarized Light
Proteomic Analysis of Caenorhabditis elegans
Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells
Myoblast Fusion in Drosophila
Analysis of Mitochondrial Protein Synthesis in Yeast
Treatment of Murine Transplanted Subcutaneous Tumors Using Systemic Drug Administration
Imaging Lipid Membrane Domains with Lipid-Specific Probes
Clonal Analysis of Hedgehog Signaling in Drosophila Somatic Tissues
Characterizing Ligand-Microtubule Binding by Competition Methods
Spoligotyping for Molecular Epidemiology of the Mycobacterium tuberculosis Complex
Analysis of Ascarosides from Caenorhabditis elegans Using Mass Spectrometry and NMR Spectroscopy
In Vitro JAK Kinase Activity and Inhibition Assays
组织工程（histological engineering）
Isolation of Adult Hippocampal Neural Progenitors
In Vivo Cryotechniques for Preparation of Animal Tissues for Immunoelectron Microscopy
SOFT AGAR ASSAY FOR COLONY FORMATION
Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in He
Assays to Measure p53-Dependent and -Independent Apoptosis
Serial Cultivation of Primary Adult Murine Keratinocytes
Mammalian Cell Microinjection Assay to Study the Function of Rac and Rho
Base Excision Repair Assay Using Xenopus laevis Oocyte Extracts
Instrument-Independent Software Tools for the Analysis of MSMS and LCMS Lipidomics Data
Genome-Wide RNAi Screening to Identify Regulators of Oncogene-Induced Cellular Senescence
Functional Purification of Human and Mouse Mammary Stem Cells
Stem Cell and Neuron Co-cultures for the Study of Nerve Regeneration
Maintenance of Pregnancy in Pigs With Limited Viable Embryos
Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells
Mechanism of Transdermal Drug Delivery by Electroporation
Mathematical Investigation of How Oncogenic Ras Mutants Promote Ras Signaling
Parthenogenesis and Nuclear Transfer in Rabbit Oocytes
Parasexual Genetics Using Axenic Cells
Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein
Methods for Detecting Cells in S Phase
Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry
Protein Expression Profiling
Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcripti
A Genome-wide RNAi Screen for Polypeptides that Alter rpS6 Phosphorylation
b-Galactosidase Color Assay for Cultured Cells
Isolation of Oligodendroglial Cells from Cultured Neural Stem/Progenitors
Derivation and Maintenance of Human Embryonic Stem Cells
Derivation of Mouse Embryonic Stem Cell Lines from Blastocysts Produced by Fertilization and Somatic
Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells
Peptide Structure Determination by NMR
A first step in defining the function of a novel gene is to determine its interactions with other ge
Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
Human Embryonic Stem (ES) Cell Protocols――Splitting Human ES cells on MEFs
Vascular Progenitor Cell Mobilization
Cytochemical Probes of Cycling and Quiescent Cells Applicable to Flow Cytometry
Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization
Quantitative Polymerase Chain Reaction
Chromosome Spreading and Immunofluorescence Methods in Saccharomyes cerevisiae
Fluorescence-Activated Cell Sorting Analysis of Mitochondrial Content, Membrane Potential, and Matri
Glycosylation Profiling of Heterologous Proteins
Mouse Epidermal Keratinocyte Culture
In Vitro Spermatogenesis Using an Organ Culture Technique
Serum Thawing Heat Inactivation
Bacterial Phagosome Acidification Within IFN--Activated Macrophages: Role of Host p47 Immunity-Relat
-Tubulin and Microtubule-Binding Assays
Methods for Cancer Stem Cell Detection and Isolation
动物细胞的基本形态观察和显微测量
In Vitro Selection of Protein and Peptide Libraries Using mRNA Display
Single-Cell PCR Methods for Studying Stem Cells and Progenitors
Mathematics of Experimentally Generated Chemoattractant Gradients
Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture
Visual Assessment of Enzyme Immobilization
Quantitation of Methylmalonic Acid in Plasma Using Liquid Chromatography Tandem Mass Spectrometry
Nuclear Transfer in Sheep
Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extracts
Conjunctiva: Organ and Cell Culture
Combing Genomic DNA for Structural and Functional Studies
神经胶质细胞的制备和培养
Generation and Cloning of Antigen-Specific Human T-Cells
Placenta Trophoblast Fusion
Human Female Reproductive Tract Epithelial Cell Culture
Prospective Isolation of Mesenchymal Stem Cells from Mouse Compact Bone
Culture of Glial Cells from Human Brain Biopsies
Bistability in One Equation or Fewer
Selective Antagonism of Receptor Signaling Using Antisense RNA to Deplete G-Protein Subunits
Palatal Dysmorphogenesis: Quantitative RT-PCR
Primary Culture of Human Renal Proximal Tubule Epithelial Cells and Interstitial Fibroblasts
Study of G Protein-Coupled Receptor/-arrestin Interactions Within Endosomes Using FRAP
Blue Native Polyacrylamide Gel Electrophoresis: A Powerful Diagnostic Tool for the Detection of Asse
Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells
Screening for MAPK Modulators Using an In-Cell Western Assay
Efficient PCR Production of Single-Stranded DNA Sequencing Templates
酵母遗传学方法3：RNA的分离
Differentiation of Circulating Monocytes into Fibroblast-Like Cells
PARAFILM PM996封口膜
二甲基亚砜的用途是什么
如何正确的使用封板膜
Parafilm封口膜的使用
Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development
Maintenance of Cell Culture
大鼠氧化低密度脂蛋白(OxLDL)酶联免疫分析（ELISA）
Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase
A Stochastic PCR Approach for RNA Quantification in Multiple Samples
Quantitative Analysis of Human DNA Sequences by PCR and Solid-Phase Minisequencing
FACS Analysis of the Planarian Stem Cell Compartment as a Tool to Understand Regenerative Mechanisms
Culture Systems: Single Step
Fractionation of the Coxiella burnetii Parasitophorous Vacuole
Designing, Testing, and Validating a Microarray for Stem Cell Characterization
Spermine Synthase Deficiency Resulting in X-Linked Intellectual Disability (SnyderRobinson Syndrome)
基因扩增(gene amplification)
Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2
Quantitative Analysis of JAK Binding Using Isothermal Titration Calorimetry and Surface Plasmon Reso
Isolation, Expansion, and Characterization of Human Islet-Derived Progenitor Cells
Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Open
ECM Degradation Assays for Analyzing Local Cell Invasion
Analyzing Import Intermediates of Mitochondrial Proteins by Blue Native Gel Electrophoresis
Managing Apheresis Complications During the Hematopoietic Stem Cell Collection
Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)
A Historical Perspective of the EGF Receptor and Related Systems
Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-T
核纤层(lamina)
磷脂交换蛋白(phospholipid exchang proteins, PEP)
Quantitative X-ray Imaging of Rodent Bone by Faxitron
Mechanical Properties of Bone Ex Vivo
Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis
Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation
Freezing and Thawing Cultured Cells
The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells
In Vitro Culture of Rodent Embryos During the Early Postimplantation Period
Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets
Crosstalk Coregulation Mechanisms of G Protein- Coupled Receptors and Receptor Tyrosine Kinases
Imaging Ca2+ Signals in Xenopus Oocytes
Identification of MetaboliteRiboswitch Interactions Using Nucleotide Analog Interference Mapping and
Reactive Oxygen Species and Upregulation of NADPH Oxidases in Mechanotransduction of Embryonic Stem
In Vitro Excision Repair Assay in Schizosaccharomyces pombe
Generation of iPS Cells from Human Umbilical Vein Endothelial Cells by Lentiviral Transduction and T
Subculturing Cells
Signal Transduction Inhibitors in Cellular Function
抑瘤粉抗肝部细胞实验研究
Harvesting lymphocytes from Peripheral Blood
Making Protein Immunoprecipitates
Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization
Intravital Imaging of Thrombus Formation in Small and Large Mouse Arteries: Experimentally Induced V
Statistical Inference on Categorical Variables
Microfluidic Techniques for the Analysis of Bacterial Chemotaxis
小鼠粒细胞-巨噬细胞集落刺激因子（GM-CSF）酶联免疫分析
Using Surface Electrodes to Monitor the Electric-Pulse-Induced Permeabilization of Porcine Skin
Derivation and Culture of Mouse Trophoblast Stem Cells In Vitro
Investigating the Origins of Somatic Cell Populations in the Perinatal Mouse Ovaries Using Genetic L
Production of Zebrafish Germline Chimeras from Cultured Cells
Cloning and Mutational Analysis of the Shc-Phosphotyrosine Interaction/Phosphotyrosine-Binding Domai
Use of Chemical Mutagenesis in Mouse Embryonic Stem Cells
Electron Spectroscopic Imaging of the Nuclear Landscape
A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity
Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques
StemBase: A Resource for the Analysis of Stem Cell Gene Expression Data
Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing
Methods for Analyzing MicroRNA Expression and Function During Osteogenic Differentiation of Human Ad
Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization
Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging
亮氨酸拉链基元(leucine zipper motif)
Detection of Apoptosis in Drosophila
Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis
The Use of Real-Time Quantitative PCR for the Analysis of Cytokine mRNA Levels
Solid-Phase Screening Using Digital Image Analysis
Practical Protocols for Lipase Immobilization via SolGel Techniques
Methods for Studying Activation of Matrix Metalloproteinases
大鼠血红蛋白(Hb)酶联免疫分析（ELISA）
Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies
Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Bloo
In Situ Hybridization of Suprachiasmatic Nucleus Slices
15种细胞培养中常用培养基及基本特性
Real-Time BRET Assays to Measure G Protein/Effector Interactions
G418原代细胞的筛选的Protocol
Heme Oxygenase Activity Current Methods and Applications
CHO Centrosome Prep
The GTPase Cycle How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases
In Vitro Human Skin Segmentation and Drug ConcentrationSkin Depth Profiles
Generation of Parabiotic Mice for the Study of DC and DC Precursor Circulation
Human Embryonic Stem (ES) Cell Protocols――Freezing Human ES Cells
Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis
Biolistic Mediated Production of Transgenic Oil Palm
Extraction of Yeast Mitochondrial Membrane Proteins by Solubilization and Detergent/Polymer Aqueous
合子或起始减数分裂（Zygotic or initial meiosis）
Processivity of DNA Repair Enzymes
Since the discovery of RNA interference (RNAi) in Caenorhabditis elegans in 1998, this mechanism has
The RNA Silencing Technology Applied by Lentiviral Vectors in Oncology
Methods of Cellular Senescence Induction Using Oxidative Stress
Generation of Human Osteoclasts from Peripheral Blood
Identification and Quantitation of Amphetamine, Methamphetamine, MDMA, Pseudoephedrine, and Ephedrin
ced-9基因（ced-9 gene）
Production of Immortalized Human Neural Crest Stem Cells
Analysis of Properties of Cilia Using Tetrahymena thermophila
Methods to Fuse Macrophages In Vitro
Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
cenM-FISH Approaches
Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium
Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases
Measuring Interactions Between ECM and TGF-Like Proteins
Dynamic Expansion Culture for Mesenchymal Stem Cells
Cell Viability Assay
染色体分带技术
Density Gradient Separation of Sperm for Artificial Insemination
DNA分子标记在三系杂交水稻育种、品种鉴定、遗传性分析上的应
Methods for Identifying and Studying Genetic Alterations in Hormone-Dependent Cancers
Radioactive In Situ Hybridization to Animal Chromosomes
The Transformed Phenotype
胸腹水染色体的标本制备
线粒体荧光探针信息大全
ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays
专门用于冻存哺乳细胞培养基
脂质体2000可以转染Hela细胞吗
Angiogenic Signalling Pathways
GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro
Isolation of Nucleic Acids
Feeder-Free Substrates for Pluripotent Stem Cell Culture
Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-
胞质分裂(cytokinesis)
Screening Expression Libraries with Solution-Based Assay
Ras GTPases Are Both Regulators and Effectors of Redox Agents
Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies
停靠蛋白(docking protein, DP)
Laboratory Guidelines for Animal Care
Differentiate ES cells into cystic embryoid bodies
Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo
Apoptosis: Miniassay
Senescence Regulation by the p53 Protein Family
Molecular Profiling of the Epidermis: A Proteomics Approach
Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies
Phenotypic Characterization of Parp-1 and Parp-2 Deficient Mice and Cells
Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells
Screening for Inhibitors of Microtubule-Associated Motor Proteins
Pathways Implicated in Stem Cell Migration: The SDF-1/CXCR4 Axis
球体细胞培养
Interactions of Amphipathic CPPs with Model Membranes
选择最适合的细胞增殖检测方法
Functionality Screen of Streptavidin Mutants by Non-Denaturing SDSPAGE Using Biotin-4-Fluorescein
细胞的分裂指数
Evaluation of Tankyrase Inhibition in Whole Cells
提高U251的转染效率方法
Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting in
HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminex Techno
Analysis of Meiotic Recombination in Caenorhabditis elegans
ChIP-Enriched in Silico Targets (ChEST), a ChIP-on-Chip Approach Applied to Analyzing Skeletal Muscl
Microspread Ovarian Cell Preparations for the Analysis of Meiotic Prophase Progression in Oocytes wi
In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduc
Lentiviral Vector-Mediated Expression of pre-miRNAs and AntagomiRs
Determination of Phosphoinositide Binding to K+ Channel Subunits Using a ProteinLipid Overlay Assay
Volatile Oxylipins and Related Compounds Formed Under Stress in Plants
Melanoblasts as Multipotent Cells in Murine Skin
Developmental Biology Protocols: Overview II
微管组织中心(microtubule organizing centers, MTOC)
相差观察和摄影
Use of Gene Overexpression to Assess Function in Cell Cycle Control
Using an Inverse PCR Strategy to Clone Large, Contiguous Genomic DNA Fragments
Cell Cycle Analysis of Asynchronous Populations
Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale
Methods for Determining the Proliferation of Cells in Response to EGFR Ligands
Splice Redirection as a Convenient Assay to Monitor CPPON Efficiency and Mechanism
Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue
The Reconstitution of Actin Polymerization on Liposomes
Measuring Angiogenesis in Mice
Peptidomic Analysis of Single Identified Neurons
圆球体(spherosome)
FISH Methods in Cytogenetic Studies
Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells
Sponge Implant Model of Angiogenesis
Microtubule Spindowns for Visual Analysis
The Measurement of Cyclic AMP Levels in Biological Preparations
Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches
Studying Mechanosensitive Ion Channels Using Liposomes
Genetic-Engineered Models of Skeletal Diseases II: Targeting Mutations into Transgenic Mice Chondroc
血影蛋白(spectrin)
PACE Analysis of RNA-Peptide Interactions
分泌（secretion）
Generation and Characterization of Epiblast Stem Cells from Blastocyst-Stage Mouse Embryos
Goat Embryonic Stem-Like Cell Derivation and Characterization
Measurement of Autophagy in Cells and Tissues
Quantification of Redox Conditions in the Nucleus
Methods for Characterization/Manipulation of Human Corneal Stem Cells and their Applications in Rege
Using Multiphoton Excitation to Explore the Murky Depths of Developing Embryos
Mobilization of Hematopoietic Stem/Progenitor Cells: General Principles and Molecular Mechanisms
The Role of Receptor Tyrosine Kinases in Primordial Germ Cell Migration
High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidases
Transdermal Delivery Using Surface Electrodes in Porcine Skin
hTERT Knockdown in Human Embryonic Kidney Cells Using Double-Stranded RNA
Application of Genetically Modified Feeder Cells for Culture of Keratinocytes
Cell Freezing
Membrane Protein Expression in Cell-Free Systems
乙醇酸途径(glycolate pathway)
Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes
Application of Antisense Oligodeoxynucleotides in Developing Chick Embryos
Detection of Meiotic DNA Breaks in Mouse Testicular Germ Cells
Identification, Assay, and Functional Analysis of the Antizyme Inhibitor Family
In Vivo Transplantation Assay at Limiting Dilution to Identify the Intrinsic Tissue Reconstitutive C
Primary Culture of Human Antral Endocrine and Epithelial Cells
Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations
Using the Yeast Genome-Wide Gene-Deletion Collection for Systematic Genetic Screens
Spectrofluorometric Assay for Cell-Tissue Electrofusion
Post-translational Control of ETS Transcription Factors: Detection of Modified Factors at Target Gen
Measurement of Plasma/Serum Acylcarnitines Using Tandem Mass Spectrometry
兔结缔组织生长因子（CTGF）酶联免疫分析（ELISA）
国外细胞株购买指南
Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus
Expression of an Integral Membrane Protein, the 5HT5A Receptor
Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells
Negative Stain Electron Microscopy of Microtubules
Assessment of Laterality Defects in Rodent Embryos
Steroid Hormone Metabolites and Hormone Binding Assays
Live to Dead Cell Imaging
Studies on Coagulation Incompatibilities for Xenotransplantation
无蛋白培养基与限定化学成分培养基的区别
From ES Cells to Mice: The Gene Trap Approach
Amplified Assay for Specific Dual-Labeled DNA Using the Coagulation Cascade (EDNA-ELCA)
Qualitative and Quantitative Analyses of Phospholipids by LCMS for Lipidomics
CO2培养箱的选购和使用技巧
Cultures of Adipose Precursor Cells and Cells of Clonal Lines from Animal White Adipose Tissue
Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives
hESC Engineering by Integrase-Mediated Chromosomal Targeting
Analyzing Planar Cell Polarity During Zebrafish Gastrulation
Analysis of Global Gene Expression Profiles Activated by Chemoattractant Receptors
Analysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cel
Viability Cell Count
Imaging Podosome Dynamics and Matrix Degradation
Isolation of Rat Telencephalic Neural Explants to Assay Shh GABAergic Interneuron Differentiation-In
Two-Photon Fluorescence Imaging and Reactive Oxygen Species Detection Within the Epidermis
Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery
A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in
Analysis of Fiber-Type Differences in Reporter Gene Expression of -Gal Transgenic Muscle
Enhanced Immunohistochemical Resolution of Claudin Proteins in Glycolmethacrylate-Embedded Tissue Bi
食蟹猴白细胞介素-6（IL-6）酶联免疫分析（ELISA）
二羧酸转运载体(dicarboxylate exchange carrier)
Retroviral Transduction of Murine Primary T Lymphocytes
High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450
Lipid Profiling Using Two-Dimensional Heteronuclear Single Quantum Coherence NMR
烟酰胺乙酰胆碱受体在凋亡中的作用通路图
电子载体(electron carriers)
Knock-in and Knock-out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways
大鼠路氏锥虫IgG抗体酶联免疫分析（ELISA）
Culturing Mouse Embryonic Fibroblasts
大鼠肌酸激酶(CK)酶联免疫分析（ELISA）
周期蛋白(cyclin)
Protein (Western) Blotting with Immunodetection
The Role of the Adapter Protein SHB in Embryonic Stem Cell Differentiation Into the Pancreatic -Cell
细胞生物基本方法：上皮细胞培养
Direct tissue explant method for derivation and study of extravillous
腹腔巨噬细胞吞噬功能检测方法
Stock solutions for tissue culture
体细胞Barr小体观察
Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA
Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL
Electron Microscopic Immunogold Localization of Recombination-Related Proteins in Spreads of Synapto
Immunohistochemistry and Detection of Proliferating Cells by BrdU
High-Throughput Screens to Discover Synthetic Riboswitches
Isolation and Characterization of Human Fetal Myoblasts
Hypoxic Gene Regulation in Differentiating ES Cells
Time-Lapse Epifluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oo
基因的连锁与交换
转染细胞不表达怎么补救
Force Generation by Dynamic Microtubules In Vitro
Directing Epidermal Fate Selection by a Novel Co-Culture System
Xenotransplantation: An Overview of the Field
卵母细胞(oocyte)
Modulating and Targeting Meiotic Double-Strand Breaks in Saccharomyces cerevisiae
Use of Differentiating Embryonic Stem Cells in Pharmacological Studies
Protocols for Peptidomic Analysis of Spider Venoms
Invasion Assays and Matrix Metalloproteinases: Quantification of Cellular Invasion Using Propidium I
pc12培养经验
染色体(chromosome)
Umbilical Cord Blood Cells
Electron Microscopic Studies of Receptor Localization
Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters
Measurement of Low-Molecular-Weight Carboxylic Acids in Ambient Air and Vehicle Emission by Capillar
Mouse Tissue Culture Models of Unstable Triplet Repeats
Thin-Layer Chromatography of Phospholipids
Fluorescence In Situ Hybridization on Single Cells: Sex Determination and Chromosome Rearrangements
Release Testing of Retroviral Vectors and Gene-Modified Cells
A Cleanup Method for Mass Spectrometry of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a
裸鼠成瘤肿瘤长不出来怎么办?
做免疫组化时脱片怎么办？
免疫荧光结果背景强怎么办？
巨噬细胞纯化的方法有哪些？
流式分选和磁珠分选有什么区别？
免疫荧光定位不对怎么办？
什么是油红O染色？
油红O染色实验步骤与注意事项
【国自然热点文献】之乳酸化（三）
石蜡切片切不出蜡带怎么办？
什么是细胞瞬时转染？
什么是细胞稳定转染？
细胞稳定转染和瞬时转染有什么区别？
HE染色细胞核暗淡怎么办？
透射电镜看外泌体的哪些表征？
细胞瞬时转染和细胞稳定转染，两者的区别？
什么是质粒
质粒抽提的详细实验步骤是怎样的？
SNP变异后为什么有两种碱基？
单细胞悬液有什么作用？
做组织切片为什要固定？如何固定？
石蜡包埋时要注意什么？
双安荧光素酶转染率低怎么办？
做COIP没有想要的目的条带怎么办？
为什么PAS染色很淡？具体原因及解决方法
免疫荧光信号不真实的原因及解决办法
western blot检测实验常见问题及解决方法
tunel染色结果怎么分析，每个结果的异常原因及调整方法
动物造模血管功能实验步骤
原代细胞转染技巧
细胞焦亡检测金标准
常见的细胞实验有哪些
cck8检测原理和实验步骤
细胞诱导分化是怎样的？
质粒提取的方法以及原理和常见问题
番红固绿染色原理，实验步骤以及需要注意什么？
什么是FISH技术？
FISH染色实验步骤
WB实验原理及流程图
WB实验检测介绍——检测小鼠组织中GSTM2的表达
实时荧光定量 PCR（qPCR）的介绍
实时荧光定量pcr的计算
一看就会的油红O染色实验指南
为什么质粒转染荧光检测会失败？
质粒转染荧光检测失败后的解决办法
做酶联免疫吸附剂测定实验需要注意什么？
关于SNP检测流程和应用
双荧光素酶报告测定的方法
双荧光素酶检测实验步骤
双荧光素酶检测结果是怎么分析的？
什么是CHIP技术？——CHIP技术原理
CHIP实验步骤
GST pulldown实验步骤
GST pulldown实验的需要注意什么？
GST pulldown实验结果分析
酵母双杂交的实验原理和步骤
酵母双杂交实验采用的系统有哪些？
酵母双杂交的实验设计是怎么样的？
酵母双杂交实验技术的作用
RIP检测实验流程
RIP实验设计方案是怎样的？
冰冻切片和石蜡切片有所不同？
怎样进行免疫共沉淀实验的分析？
免疫共沉淀—实验操作流程和步骤
免疫共沉淀（Co-IP）实验技术
GST-puiidown和coip有何区别？
RNA pull-down实验原理介绍+实验步骤
RNA pull-downll实验结果怎么看？
RNA pull-downll实验结果分析
什么是 northern blot技术？
northern blot实验详细操作报告
northern blot实验作用和原理
southern blot实验目的是什么？
southern blot实验流程
southern blot原理和应用
EMSA实验概念和原理
EMSA实验的步骤
在进行EMSA实验为什么看不到迁移带？
EMSA实验结果要怎么看？
EMSA测定需要多少量的蛋白标记的探针？
EMSA实验过程膜没有一直处于湿润状态怎么办？
Dot Blot实验方法以及操作步骤
Dot Blot实验结果分析报告是怎样的？
Dot Blot实验和western blot实验有何区别？
为什么细胞会衰老？主要原因有哪些？
细胞衰老的主要特征
血管生成的基本过程是怎样的？
血管生成的原因和方式
血管生成的相关实验和疾病有哪些？
血管形成实验操作步骤
血管生成的指标
常做的细胞实验有哪些？
肿瘤细胞成球实验
细胞焦亡的机制
常用的细胞焦亡检测方法包括？
细胞焦亡是程序性死亡吗？
细胞早期凋亡和晚期凋亡的区别？
细胞凋亡和焦亡坏死的区别？
免疫表型检测的目的
免疫表型是什么？
免疫表型检测报告怎么看？
细胞融合与培养细胞的形态观察和计数
猴B病毒抗体（BV-Ab）酶联免疫分析（ELISA）
CELL CYCLE ANALYSIS
Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents for Studying Membrane Proteins
Immune Ablation Followed by Autologous Hematopoietic Stem Cell Transplantation for the Treatment of
肌钙蛋白(troponin，Tn)
In Vitro Analyses of Endothelial Cell Permeability
Animal Models of Multiple Sclerosis
中期(metaphase)
The CE Way of Thinking: All is Relative!
Multilocus Enzyme Electrophoresis for Parasites and Other Pathogens
MMPs and TIMPs: An Historical Perspective
Chemotaxis of Slow Migrating Mammalian Cells Analysed by Video Microscopy
Cell Arrays for the Measurement of Organelle Dynamics in Living Cells
Detection and Quantification of Cocaine and Benzoylecgonine in Meconium Using Solid Phase Extraction
Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption
Serial Analysis of Gene Expression in Human Keratinocytes and Epidermis
Apoptosis in the Epidermis
细胞培养中的“黑胶虫”问题
CDC25 Dual-Specificity Protein Phosphatases: Detection and Activity Measurements
细胞遗传学――原位引物启动技术(PRINS)
Blue Native-Gel Electrophoresis Proteomics
Construction and Purification of Adhesion Molecule Immunoglobulin Chimeric Proteins
In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells
Parallel Detection of Crossovers and Noncrossovers in Mouse Germ Cells
人胸腺基质淋巴细胞生成素 (TSLP)酶联免疫分析（ELISA）
Differentiation and Characterization of Human MSCs
Studying Gene Expression in Bone by In Situ Hybridization
RNA Footprinting and Modification Interference Analysis
Analytic Methods for Modeling Stochastic Regulatory Networks
The Double Immunodiffusion Technique: Immunoprecipitation and Analysis of Antigenic Protein in Gel
Cupric Ion Reducing Antioxidant Capacity Assay for Food Antioxidants: Vitamins, Polyphenolics, and F
A Novel Experimental Platform for Investigating Cancer Growth and Anti-Cancer Therapy in a Human Tis
Assay to Measure Oxidized and Reduced Forms of CoQ by LCMS/MS
A Sensitive and Versatile Cytokine Bioassay Based on Type I Interferon Signaling in 2fTGH Cells
Electroporation of Adherent Cells In Situ for the Introduction of Nonpermeant Molecules
Affinity Peptidomics: Peptide Selection and Affinity Capture on Hydrogels and Microarrays
Use of Liposomes Containing Carbohydrates for Production of Monoclonal Antibodies
The chick embryo is easily accessible and has therefore been widely used in developmental biology st
Time-Lapse Imaging of Chick Cardiac Precursor Cells
Quantitative Analysis of Distal Tip Cell Migration in C. elegans
Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens
Immunocytochemistry to Study Myogenesis in Zebrafish
Reversible Covalent Immobilization of Enzymes via Disulfide Bonds
Gene Mapping to Chromosomes by Hybridization in Situ
大鼠细小病毒抗体(RPV)酶联免疫分析
A New Approach to Primary Culture of Human Gastric Epithelium
TAT-Peptide Modified Liposomes: Preparation, Characterization, and Cellular Interaction
Production and Purification of Monoclonal Antibodies
Monitoring and Analyzing Drosophila Circadian Locomotor Activity
Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos
Isolation and Culture of Human Osteoblasts
Detection of Cell Death by Autophagy
Analysis of Rhythmic Gene Expression in Adult Drosophila Using the Firefly Luciferase Reporter Gene
大鼠apelin (AP)酶联免疫分析（ELISA）
Expression and Analysis of Exogenous Proteins in Epidermal Cells
Ribonuclease Inhibitors
囊胚（blastula）
小鼠核因子kb受体活化因子配基（RANKL）酶联免疫分析
Methods to Study MYC-Regulated Cellular Senescence
Isolation of cell nuclei for the application in the cell-free system
无血清培养基与完全培养基体外诱导扩增 CIK细胞
巨噬细胞原代培养
数量性状的统计分析实验
TUNEL assay
人白细胞介素-18（IL-18）酶联免疫分析（ELISA）
Flow Sorting Fibroblasts with GFP and P.I.
Sequencing PCR Products Cloned into M13 Vectors
基质(ground substance)
大批量生产干细胞培养基的具体细节
Proteolytic Enzymes for Peptide Production
Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts
Assay for Neuronal Cell Migration
配体(ligand)
细胞介导细胞毒作用检测法5：用MTT检测法测定CMC
大鼠免疫球蛋白E(IgE)酶联免疫分析（ELISA）
Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse Microscopy
微球体体系
Preparation of Myristoylated Arf1 and Arf6 Proteins
In Situ Localization of PCR-Amplified DNA and cDNA
Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
Differential Expression of TIMP-3 During Neoplastic Progression in the Mouse JB6 Model System
Human Embryogenesis: Overview
Electrophoresis of Cerebrospinal Fluid
胞质环流(cytoplasmic streamting)
TGF- Signaling in Embryonic Stem Cell-Derived Endothelial Cells
Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes
Human Hepatocytes: Isolation, Culture, and Quality Procedures
Transcription Factor Sequestration by Polyglutamine Proteins
Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells
BiotinProtein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applica
Biocompatible Nanoparticle Labeling of Stem Cells and Their Distribution in Brain
免疫表型研究分析
CCK8检测实验报告和详细的实验步骤
CCK-8实验基本步骤以及注意事项
CCK8细胞增殖率怎么计算分析？
CCK8检测细胞增殖的目的以及方法
mtt实验与CCK8实验的区别？
细胞划痕实验原理以及步骤
细胞划痕用什么拍照？
细胞划痕实验说明什么？
细胞划痕实验是怎么分析的？
细胞划痕实验结果计算公式
在进行细胞划痕实验中有什么技巧？
什么是细胞侵袭实验？以及该实验前的准备
细胞侵袭实验步骤
细胞侵袭实验结果怎么读？
轻松鉴别细胞侵袭和迁移
单细胞多组学完整解决方案
细胞侵袭实验常见问题以及解决办法
在细胞实验中还有哪些常见的细胞侵袭实验？
激光共聚焦的优点是什么？以及具体的实验流程
植物各器官冰冻切片最适宜温度是多少？
什么是克隆形成？
克隆形成实验报告和实验操作步骤
克隆形成实验操作注意事项
克隆形成有哪些分类和优点？
原代细胞分离培养与鉴定有哪些优点？
In Vitro Culture of Fetal Ovaries: A Model to Study Factors Regulating Early Follicular Development
Claudins in a Primary Cultured Puffer Fish (Tetraodon nigroviridis) Gill Epithelium
SAMe and HuR in Liver Physiology: Usefulness of Stem Cells in Hepatic Differentiation Research
Microarray Technology for Mutation Analysis of Low-Template DNA Samples
B-Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization
Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and Martigel Gels
High-Throughput Screening of Microtubule-Interacting Drugs
Screen for Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase
流式细胞仪在睡眠性阵发性血红蛋白尿的诊断中的应用
Counting Chromosomes in Intact Eggs
Viral Transformation of Epithelial Cells
The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of P
Genome-Wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq
Determination of Proteasomal Activities
Sequencing-Based Typing of HLA
Tumor Growth Fraction Estimation, Perturbation, and Prognostication
Methods for Studying Spindle Assembly and Chromosome Condensation in Xenopus Egg Extracts
Receptor Knock-Out and Knock-In Strategies
Pre-embedding Nanogold Silver and Gold Intensification
Purification of Native Bone Marrow Megakaryocytes for Studies of Gene Expression
Correlative Transmission Microscopy: Cytochemical Localization and Immunocytochemical Localization i
Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard
Lysosome Isolation in Isotonic Sucrose
Assessment of LiposomeCell Interactions
Phenotypic Analysis and Differentiation of Murine Mesenchymal Stem Cells
通过细胞融合得到B细胞杂交瘤实验
viral immunity pathogenesis group
Identification and Analysis of Epidermal Stem Cells from Primary Mouse Keratinocytes
The Isolation and Culture of Human Cord Blood-Derived Mesenchymal Stem Cells Under Low Oxygen Condit
Intravital Microscopy to Study Leukocyte Recruitment In Vivo
Generation of a Tetracycline Regulated Mouse Model of MYC-Induced T-Cell Acute Lymphoblastic Leukemi
High-Resolution Capillary Gas Chromatography in Combination with Mass Spectrometry for Quantificatio
Diagnosis of Creatine Metabolism Disorders by Determining Creatine and Guanidinoacetate in Plasma an
Neonatal Testicular Gonocytes Isolation and Processing for Immunocytochemical Analysis
原代细胞和传统细胞培养的区别
Isolation of Lipid Rafts From B Lymphocytes
原代细胞培养失败的原因
Cell Senescence as Both a Dynamic and a Static Phenotype
原代细胞培养注意事项
原代细胞分离培养与鉴定有哪些优点？
生物实验设计原则及需警惕的科研逻辑谬误
什么是EDU检测细胞增殖？
EdU检测实验操作步骤——及荧光定量分析数据
EDU检测细胞增殖结果如何分析？
了解细胞增殖的新视角 —— 检测EDU技术的方法
细胞增殖检测之EdU法及其应用
什么是外泌体提取？
外泌体提取的具体方法及步骤
如何正确提取外泌体
外泌体提取实验常见方法以及优缺点
如何构建和生成耐药细胞系？
什么是shRNA载体构建？
shRNA载体构建有哪些不同类型？
shRNA载体构建实验的详细过程
shRNA载体构建的优缺点
shRNA载体构建注意事项
介绍CRISPR/Cas9载体构建
CRISPR/Cas9载体构建的详细步骤和过程
CRISPR/Cas9载体构建技术要点及流程
CRISPR/Cas9载体构建技术特点与应用
CRISPRCas9载体构建相关问题和注意事项
分子克隆的定义
分子克隆：从基本概念到实验流程
解锁核孔蛋白NUP210的力量：通过破坏肿瘤细胞的力学响应，抑制转移
分子克隆里基因到蛋白质的五个关键步骤
分子克隆的方法有哪些？
分子克隆注意事项及应用实例分析
分子克隆和亚克隆的区别？
定点突变的定义
了解PCR定点突变的原理
定点突变技术方法有哪些？
定点突变实验和定向突变的区别
PCR定点突变实验遇到的问题和解决办法
什么是质粒提取？
质粒提取平衡的原理
质粒提取原理及操作步骤
质粒提取和纯化的原理
质粒提取常见问题以及解答
质粒提取的目的
慢病毒包装的原理
慢病毒包装系统介绍
慢病毒包装系统应用和步骤
慢病毒包装常见问题及解决方案
慢病毒包装的注意事项
裸鼠皮下成瘤实验技巧和方法
Isolation of Plant Mitochondrial RNA from Green Leaves
Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embry
Human Myoblasts and Muscle-Derived SP Cells
In Situ Detection of microRNAs in Animals
小鼠骨髓细胞染色体标本制作[细胞遗传]
Gene Silencing for Functional Analysis: Assessing RNAi as a Tool for Manipulation of Gene Expression
A Review of Post-translational Modifications and Subcellular Localization of Ets Transcription Facto
Statistical Topics in the Laboratory Sciences
Investigating Gene Expression: In Situ Hybridization and Reporter Genes
Ribosomal Initiation Complexes Probed by Toeprinting and Effect of trans-Acting Translational Regula
Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis
Electroporation of ES cells
Refolding of TIMP-2 from Escherichia coli Inclusion Bodies
Human Embryo Culture Media Comparisons
Preparation of Intact, Highly Purified Phagosomes from Dictyostelium
Solid Tissue Dispersal for Cytokinetic Analyses
RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues
Transient Expression of Epitope-Tagged Proteins in Mammalian Cells
Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)
染色体CBG标本的制备
Urothelial Cell Culture: Stratified Urothelial Sheet and Three-Dimensional Growth of Urothelial Stru
In Situ Biosynthesis of Peptide Arrays
细胞培养染色体显示
Physical Properties of the Nucleus Studied by Micropipette Aspiration
Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections
李斯特氏菌在细胞间传播方式
Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes
流式样本的固定和保存
Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture
Analysis of Gene-Specific and Genome-Wide Sperm DNA Methylation
关于乳鼠心肌细胞培养的一点收获
Quantitation of Tacrolimus in Whole Blood Using High Performance Liquid Chromatography-Tandem Mass S
In Vivo Imaging of Oligonucleotidic Aptamers
Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes
Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity
Neural Transplantation and Stem Cells
Determination of Gene Expression Patterns by In Situ Hybridization in Sections
Gene Modification with Hapaxoterministic Restriction Enzymes: Easing the Way
Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix
Manipulating the Avian Epiblast and Epiblast-Derived Stem Cells
Isolating Aptamers Using Capillary ElectrophoresisSELEX (CESELEX)
Expansion of Pluripotent Stem Cells in Defined, Xeno-Free Culture System
Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells
配子或终末减数分裂（Gametic or terminal meiosis）
暗反应(dark reaction)
Ultrasound Backscatter Microscopy of Mouse Embryos
Biosensor-Based Evaluation of Liposomal Binding Behavior
Semiquantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Motoneur
Kinetic Analysis of the Inhibition of Matrix Metalloproteinases: Lessons from the Study of Tissue In
The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells
Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis
Screens for Mitochondrial Mutants in Yeast
A number of different viruses interact with the cell cycle in order to subvert host-cell function an
The Cell Cycle and Virus Infection
The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference
斑块连接 (plaque-bearing junction)
组织培养细胞染色体制备方法
In vitro culture of embryonic lungs
In Vitro Generation of Lymphocytes From Embryonic Stem Cells
$冰冻断裂复型(freeze-fracture replication)技术$
冰冻断裂复型(freeze-fracture replication)技术
Enzyme Immobilization on Polyethyleneimine-Coated Magnetite Particles
Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors
Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abun
The Application of Nuclear Magnetic Resonance to Structural Studies of Polysaccharides
裸鼠皮下成瘤实验常见操作问题以及解决办法（下）
如何巧妙设计引物？
设计引物的原则
qPCR引物设计流程
引物设计的依据
PCR设计引物的具体步骤
设计引物中有哪些小技巧？
PCR引物设计以及PCR实验细节详解
酶切位点是什么？
酶切位点是如何确定的？
Mesenchymal Stem Cell Assays and Applications
Light Microscopy in Aquatic Ecology: Methods for Plankton Communities Studies
亲和标记(affinity labeling)
非组蛋白(nonhistone proteins)
The C. elegans Cell Cycle: Overview of Molecules and Mechanisms
Ex Vivo Expansion of Human Mesenchymal Stem Cells on Microcarriers
Methods for Measuring Ligand Dissociation and Nuclear Receptor Turnover in Whole Cells
Synchronization of Medicago sativa Cell Suspension Culture
RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells
如何使昆虫细胞适应新的培养液
Studying Drug-Tubulin Interactions by X-Ray Crystallography
Recommended Experiments with Isolated Mitochondria
$Cuticle Refraction Microscopy: A Rapid and Simple Method for Imaging Drosophila Wing Topography, an$
Cuticle Refraction Microscopy: A Rapid and Simple Method for Imaging Drosophila Wing Topography, an
Polyamine Analysis by LC-MS
Methods for Studying the Nuclei and Chromosomes of Dinoflagellates
Human Endothelial and Osteoblast Co-cultures on 3D Biomaterials
Electron Microscopy and Lac-Z Labeling
RaPID: An Aptamer-Based mRNA Affinity Purification Technique for the Identification of RNA and Prote
细胞增殖的酸性磷酸酶检验法操作步骤
Immunofluorescence Staining of Human Cells by Lysed Whole Blood
Poly(ADP-Ribose) Metabolism Analysis in the Nematode Caenorhabditis elegans
Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes
Live Analysis of the Division Cycles in X-Irradiated Drosophila Embryos
Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Su
Natural Killer Cells and Killer-Cell Immunoglobulin-Like Receptor Polymorphisms: Their Role in Hemat
孢子或中间减数分裂（Sporic or intermediate meiosis）
Use of Intact Xenopus Oocytes in Nucleocytoplasmic Transport Studies
Directed Differentiation and Characterization of Genetically Modified Embryonic Stem Cells for Thera
个体发育(individual development)
Functional Assays for Human Embryonic Stem Cell Pluripotency
Comparative Physical Mapping: Universal Overgo Hybridization Probe Design and BAC Library Hybridizat
Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules: Selective Protocols of Immunization
Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microsc
Small Vectors for Expression Based on Dominant Drug Resistance with Direct Multicopy Selection
Small Animal Models of Xenotransplantation
RNA In Situ Hybridizations on Drosophila Whole Mounts
The Application of Adenoviral Vectors in the Study of Mammalian Cardiovascular Development
Assessment of Permeability in Barrier Type of Endothelium in Brain Using Tracers: Evans Blue, Sodium
肿瘤细胞的软琼脂集落培养和测定
Immunofluorescent Microscopic Study of Meiosis in Zebrafish
Flow Cytokinetics
Identification of Vertebrate Circadian Clock-Regulated Genes by Differential Display
A Quantitative Assay for Measurement of Chemokinesis in Tetrahymena
Ultrasonic Manipulation of Single Cells
Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae
Isolation of Embryonic Hematopoietic Niche Cells by Flow Cytometry and Laser Capture Microdissection
DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
High-Resolution Light Microscopic Characterization of Spermatogonia
Mammalian Target of Rapamycin: A Signaling Kinase for Every Aspect of Cellular Life
Laser Ablation and Fate Mapping
Isolation and Cultivation of Human Scalp Interfollicular Epidermal Stem Cells
Media Composition: Macromolecules and Embryo Growth
大鼠白细胞介素10（IL-10）酶联免疫分析
Genome-Wide Mapping of Meiotic DNA Double-Strand Breaks in Saccharomyces cerevisiae
Nuclear Envelope Formation In Vitro: A Sea Urchin Egg Cell-Free System
DNA Transposons for Modification of Human Primary T Lymphocytes
A Direct-Sequencing-Based Strategy for Identifying and Cloning cDNAs from Differential Display Gels
Transcriptome Analysis of Common Gene Expression in Human Mesenchymal Stem Cells Derived from Four D
Activation of Oocytes After Nuclear Transfer
Application of Sonic Hedgehog to the Developing Chick Limb
Human Thymic Epithelial Cell Cultures
Construction of Radiation Hybrid Panels
Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells
Assessment of Nanomaterials Cytotoxicity and Internalization
Optimization of the Caco-2 Permeability Assay to Screen Drug Compounds for Intestinal Absorption and
愈伤组织的诱导
Establishment and Maintenance of Normal Human Keratinocyte Cultures
同步化(synchronization)
Single Nucleotide Polymorphism Genotyping in MMP Genes: The 5 Nuclease Assay
Proteins Involved in Cell Migration from Glioblastoma Neurospheres Analyzed by Overexpression and si
Treatment of Mice with Retinoids In Vivo and In Vitro: Skeletal Staining
Electrofusion of Mammalian Oocytes and Embryonic Cells
Analysis of Bacterial Proteins by 2DE
Electroporation for Gene Therapy
Human Long-Term Bone Marrow Culture
Morphometric Evaluation of the Spermatogonial Stem Cell Distribution and Niche in Vertebrates
The Use of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embr
Intrathymic Injection for Analysis of T-Cell Progenitor Activity
Long-Term Culture-Initiating Cell Assays for Human and Murine Cells
Analysis of Phosphoinositide Dynamics During Phagocytosis Using Genetically Encoded Fluorescent Bios
High-Throughput Screens Based on NAD(P)H Depletion
Monitoring Rapid Endocytosis in the Electron Microscope via Photoconversion of Vesicles Fluorescentl
细胞转染操作步骤
Probing RNA-Protein Interactions by Psoralen Photocrosslinking
Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction
Peptidomics: Identification of Pathogenic and Marker Peptides
Application of PepFect Peptides for the Delivery of Splice-Correcting Oligonucleotides
Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver
内含信号序列(internal signal sequence)
In Situ Hybridization Using cRNA Probes: Isotopic and Nonisotopic Detection Methods
Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Proc
ENU-Based Phenotype-Driven Screening
Image Analysis of Chromatin Remodelling
Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes
Activation of p53 Protein Function in Response to Cellular Irradiation
Cryopreservation of Mouse Embryos
Isolation of Messenger RNA
Using High-Content Microscopy to Study Gonadotrophin-Releasing Hormone Regulation of ERK
Measuring the Inflammasome
Determination of Total Homocysteine in Plasma Using Liquid Chromatography Coupled to Tandem Mass Spe
Animal Models of Neurodegenerative Diseases
Detection of an mRNA Polymorphism by Differential Display
Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes
Physical Structure of Nuclear Receptor-DNA Complexes
E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression
酶切位点的详细步骤
怎么看质粒上的酶切位点？
酶切位点怎么设计？
酶切位点与识别位点有区别吗？
常见酶切位点以及保护碱基
酶切位点怎么判断？
酶切位点序列表
免疫荧光检测是什么？
免疫荧光检测原理
免疫荧光染色操作步骤以及目的——细胞篇
免疫荧光染色操作步骤以及目的
免疫荧光结果判读
免疫荧光和elisa的区别是什么？
什么是western blot？
WB检测实验报告和详细的操作步骤（中）
Purification of SR Protein Splicing Factors
Engineering of Implantable Liver Tissues
细胞表面抗原染色的组织样本降解表
Cardiac Morphogenesis and Dysmorphogenesis: An Immunohistochemical Approach
Tandem Mass Spectrometry
Purification Techniques of Subcellular Compartments for Analytical and Preparative Purposes
Single Molecule Imaging of RNA In Situ
Preparation of Mitochondria from Rat Liver
Analysis of TGF-Inducible Apoptosis
Liposomes: Applications in Protein-Lipid Interaction Studies
252-Californium Plasma Desorption Time-of-Flight Mass Spectrometry of Peptides and Proteins
Biomedical Applications of Immobilized Enzymes: An Update
Tools for Predicting Binding and Insertion of CPPs into Lipid Bilayers
Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Lay
支原体污染的不同处理方法对比
The Use of Dansyl-Calmodulin to Study Interactions with Channels and Other Proteins
Platelet Shape Change and Spreading
Studying Mycs Role in Metabolism Regulation
Respiratory and Thermogenic Capacities of Cells and Mitochondria from Brown and White Adipose Tissue
Morphometric Analysis of Centrosome Position in Tissues
Transduction of Murine Embryonic Stem Cells by Magnetic Nanoparticle-Assisted Lentiviral Gene Transf
Media Composition: Antioxidants/Chelators and Cellular Function
Using Cells Encapsulated in Agarose Microbeads to Analyse Nuclear Structure and Functions
Enzyme Analysis: Cathepsin D as an Example
Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry
Regulation of Apoptosis by the Unfolded Protein Response
Utility of Antioncogene Ribozymes and Antisense Oligonucleotides in Reversing Drug Resistance
Ex Vivo Generation of Human Red Blood Cells: A New Advance in Stem Cell Engineering
Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton
内体(endosome)
Assessment of Myocardial Angiogenesis and Vascularity in Small Animal Models
Real-Time PCR Quantification of Gene Expression in Embryonic Mouse Tissue
Atomic Force Microscopy for the Characterization of Proteoliposomes
Nuclear Transplantation and Cloning in Mammals
Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level
A High-Throughput siRNA Screening Platform to Identify MYC-Synthetic Lethal Genes as Candidate Thera
Small-Molecule Collection and High-Throughput Colorimetric Assay to Identify PARP1 Inhibitors
组成型分泌途径(constitutive secretory pathway)
Detection of NF-B Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis
Methods for Iodination of Active TGF, TGF Receptor Crosslinking and Immunoprecipitation of TGF-Recep
Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging
Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endot
Covisualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3D DNA Methylation
Methods for Analysis of Ligand-Induced RNA Conformational Changes
Sources and Derivation of Human Embryonic Stem Cells
Master Cell Bank
PNA-In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent D
Recording Ion Channels in Isolated, Split-Opened Tubules
Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test
Tiny Molecular Beacons for in vivo mRNA Detection
Saponin Lysis of RBCs
Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders
Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO
Wnt Signaling During Caenorhabditis elegans Embryonic Development
A combination of forward and reverse genetic approaches in zebrafish has revealed novel roles for ca
Wnt Signaling Mediates Diverse Developmental Processes in Zebrafish
人NK 细胞克隆的制备
Fluorescence Correlation Spectroscopy to Assess the Mobility of Nuclear Proteins
Utilization of Liposomes for Studying Drug Transfer and Uptake
染色体结构显示与检测
Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins: Part A. Preparation
Application of Laser Microdissection to Study PlantFungal Pathogen Interactions
Enzyme Immobilization by Entrapment Within a Gel Network
In Vitro Selection of Random RNA Fragments to Identify Protein-Binding Sites Within Large RNAs
Distribution of Bleomycin in a Rat Model
Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates
Small Molecule Screen in Zebrafish and HSC Expansion
The US and EU Regulatory Perspectives on the Clinical Use of Hematopoietic Stem/Progenitor Cells Gen
Routes of Stem Cell Administration in the Adult Rodent
Removing cells from liquid nitrogen
WB检测实验报告和详细的操作步骤（中）
WB检测实验报告和详细的操作步骤（下）
western blot为什么必须要用内参?
western blot条带如何看？
western blot内参怎么看？
western blot仪器介绍
western blot一抗和二抗是什么？
QPCR检测的是什么？
QPCR检测实验详细的步骤与报告（三）
QPCR原理以及数据处理
QPCR原理和主要的三个步骤
QPCR和PCR区别点和共同点是什么？
QPCR内参是什么？
QPCR实验设计
QPCR数据分析以及作图方法
核质分离实验QPCR数据处理
QPCR目的和意义
流式检测的原理以及应用
流式检测实验报告——细胞周期检测
流式细胞检测实验步骤总结
流式检测细胞周期怎么分析？
流式检测ros结果分析
流式细胞检测报告概述
流式细胞检测那些项目？
流式检测阴行对照意义
活体成像的原理
活体成像技术
小鼠活体成像的实验步骤
活体成像荧光强度定量
活体成像的注意事项
HE染色原理和意义
HE检测实验报告（一）
HE检测实验报告（二）
HE检测实验报告（三）
HE检测实验报告（四）
HE检测实验报告（五）
HE染色实验操作标准
HE染色结果分析
HE染色常见问题分析和解决方法
HE染色和免疫组化的区别是什么？
为什么HE染色切片着色浅？
he染色中出现切片或染液、组织及盖载玻片的污染怎么办？
HE染色过程中切片内有大小不一的气泡怎么办
Transwell细胞迁移
Transwell共培养
Transwell检测实验报告
Transwell迁移实验报告
Transwell侵袭实验报告
Transwell诱导单层细胞屏障
什么是Transwell转运率？
Transwell细胞计数的原理
Transwell迁移和侵袭的区别
Transwell细胞培养分选
Transwell细胞迁移实验注意事项
Transwell侵袭注意事项
什么是原位杂交实验？
荧光原位杂交检测实验报告（三）
原位杂交检测实验报告（四）
原位杂交目的
原位杂交实验分析
原位杂交实验的应用
原位杂交实验操作细节有哪些？
原位杂交常见问题的分析与对策
原位杂交方法
什么是激光共聚焦技术？
激光共聚焦实验步骤详解
激光共聚焦结果分析
激光共聚焦样品制备方法
激光共聚焦实验原理、应用以及发展是什么？
激光共聚焦实验目的
激光共聚焦和荧光共聚焦的区别
ELISA原理、操作以及注意事项
ELISA酶联免疫吸附试验报告
ELISA酶联免疫吸附试验结果分析
ELISA酶联免疫吸附试验实验目的
ELISA实验方法有哪些？
ELISA实验步骤
ELISA酶联免疫吸附试验常见问题以及解决
什么是小鼠肠炎模型？
小鼠肠炎模型构建实验报告展示
小鼠结肠炎诱导方法
小鼠肠炎模型周龄怎么算？
小鼠肠炎模型体温怎么测量？
小鼠肠炎模型对小鼠品种有什么要求吗？
小鼠肠炎模型注意事项
小鼠肠炎模型实验过程中问题和解决方法
EMSA凝胶迁移实验原理
EMSA凝胶迁移实验分类
EMSA凝胶迁移实验步骤
EMSA凝胶迁移实验电泳图分析
EMSA凝胶迁移实验结果分析
EMSA凝胶迁移实验目的
EMSA凝胶迁移实验注意事项
EMSA凝胶迁移蛋白跑不下来是什么原因？
EMSA凝胶迁移实验里BSA的作用是什么？
EMSA凝胶迁移实验过程中常见问题与解答
TUNEL染色原理介绍【组织染色】
TUNEL染色实验原理和实验操作技巧
TUNEL检测实验报告（一）
TUNEL检测实验报告（二）
TUNEL检测实验报告（三）
如何进行TUNEL染色阳性对照实验？
如何正确解读TUNEL染色结果图？
TUNEL染色实验试剂选择和配置方法
TUNEL染色和DAPI染色区别
TUNEL染色和HE染色区别是什么？
Tunnel染色实验注意事项
TUNEL染色实验在凋亡检测中的优势与局限性
TUNEL染色实验与细胞凋亡机制
TUNEL染色实验与荧光显微镜
TUNEL染色实验中的质量控制措施
TUNEL染色实验过程中的常见问题与解决方法分析
深入了解MASSON染色：原理、步骤与实例
如何进行MASSON染色？
Masson染色实验报告分析
MASSON（马松）染色方法及其在实验室的应用
MASSON（马松）染色结果怎么分析的？
MASSON（马松）染色结果怎么看纤维化？
MASSON染色和HE染色区别?
MASSON（马松）染色原理阳性意义
Detection of Copy Number Variation Using SNP Genotyping
Isolation and Culture of Human Alveolar Epithelial Cells
Using Zebrafish (Danio rerio) to Assess Gene Function in Thrombus Formation
细胞生物基本方法：结缔组织类细胞培养
Treatment of Rat Glioma With Electrochemotherapy
紫杉醇(taxol)
原肌球蛋白(tropomyosin, Tm)
Proteomics Analysis of Contact-Initiated Eph ReceptorEphrin Signaling
Identifying and Counting Protein Modifications Triggered by Nitrosative Stress
Measurement and Illustration of Immune Interaction After Stem Cell Transplantation
Antisense Therapeutics: A Promise Waiting to Be Fulfilled
Metal Analysis With Capillary Zone Electrophoresis
Culture of Preimplantation Mouse Embryos
Derivation and Characterization of Thyrocyte-Like Cells From Embryonic Stem Cells In Vitro
Receptor-DNA Interactions: EMSA and Footprinting
细胞的冻存和复苏
Virus Vectors for Gene Therapy of the Nervous System
Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation
Electrotransformation of Chinese Hamster Ovary Cells
Nanoparticles/Dip Stick
Isolation of Stromal Stem Cells From Human Adipose Tissue
Clearance of Mutant Aggregate-Prone Proteins by Autophagy
Stable Transfection Using Episomal Vectors to Create Modified Human Embryonic Stem Cells
Two-Dimensional Electrophoresis: An Overview
Antibodies Against Huntingtin: Production and Screening of Monoclonals and Single-Chain Recombinant
Immunofluorescent Localization of Proteins in Caenorhabditis elegans Muscle
Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos
人白细胞介素23（IL-23）酶联免疫分析（ELISA）
上海市临床科研基本实验技能培训-细胞培养
MASSON（马松）染色实验目的
Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract
Analysis of Soluble Sugar Permease Domains by Solution NMR
Identification and Isolation of Adult Liver Stem/Progenitor Cells
Identification of Differentially Expressed Genes in Sorted Cell Populations by Two-Dimensional Gene-
Evaluating a Screen and Analysis of Mutant Libraries
Protein Complex Analysis of Native Brain Potassium Channels by Proteomics
Large and Small Scale RNA Preparations from Eukaryotic Cells
Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens
MASSON（马松）染色实验中染液的顺序
In Vitro Generation of T Lymphocytes From Embryonic Stem Cells
Differentiation of Human Embryonic Stem Cells Toward the Chondrogenic Lineage
Using RNA FISH to Study Gene Expression During Mammalian Meiosis
Synchronization of Pathogenic Protozoans
MASSON（马松）染色实验技术注意事项
Solubilization of Aggregates Formed by Expanded Polyglutamine Tract Expression in Cultured Cells
Sequencing and Phylogenomic Analysis of Whole Mitochondrial Genomes of Animals
MASSON（马松）染色实验过程中常见问题和解决方法
Improved Chemiluminescence Assay for Measuring Antioxidant Capacity of Seminal Plasma
Transmembrane Signaling by G Protein-Coupled Receptors
PAS染色实验原理介绍
Analysis of ERKs Dimerization by Electrophoresis
Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology
PAS检测实验报告【小鼠肾】
In Vitro Analysis of the Yeast Mitochondrial RNA Polymerase
Inhibition of DNA Synthesis by Ionizing Radiation
PAS染色实验报告【小鼠心肌】
Human and Murine Skeletal Muscle Reserve Cells
Real-Time Quantitative Polymerase Chain Reaction Measurement of Male Fetal DNA in Maternal Plasma
如何获得更清晰的染色效果？
Inducing High Rates of Targeted Mutagenesis in Zebrafish Using Zinc Finger Nucleases (ZFNs)
Transient transfection into 293T cells
PAS染色的实验步骤全攻略
PAS染色结果解读
Migratory Strategies of Normal and Malignant Stem Cells
Mesenchymal Stem Cell Therapy on Murine Model of Nonalcoholic Steatohepatitis
PAS染色实验材料有哪些？
螺旋管
Flow Cytometric Monitoring of Cellular Pharmacokinetics
PAS染色和mpo染色区别是什么？
Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex
Quantitation of Oxycodone in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)
Methods for the Study of Signaling Molecules in Membrane Lipid Rafts and Caveolae
PAS染色实验注意事项
Detection of Focal Proteolysis Using Texas-Red-Gelatin
联会复合体的染色与观察
细胞支原体污染的荧光检测方法[配图]
皮层反应(cortical reaction)
轴突运输(axonal transport)
Growing cells
Media Composition: pH and Buffers
PAS染色临床应用
PAS糖原染色常见问题及解决方案
CCK8实验原理
CCK8检测实验报告（一）
CCK8检测实验报告（二）
Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes
Analyses of ProteinProtein Interactions by In Vivo Photocrosslinking in Budding Yeast
Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Sta
Cloning by Limiting Dilution of Hybridoma
Study of Apoptosis In Vitro Using the Xenopus Egg Extract Reconstitution System
Derivation and Characterization of Neuronal Precursors and Dopaminergic Neurons From Human Embryonic
Procedures for Derivation and Characterisation of Human Embryonic Stem Cells from Odense, Denmark
Multicolor Whole-Mount In Situ Hybridization
Measurement of Cholesterol in Membranes
Grafting Hensens Node
Culture of Human Normal Brain and Malignant Brain Tumors for Cellular, Molecular, and Pharmacologica
Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling
Photoactivatable (Caged) Fluorescein as a Cell Tracer for Fate Mapping in the Zebrafish Embryo
F2-Isoprostanes: Sensitive Biomarkers of Oxidative Stress In Vitro and In Vivo: A Gas Chromatography
Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis
Poly(ADP-ribosyl)ation Pathways in Mammals: The Advantage of Murine PARG Null Mutation
Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
减数分裂压片标本制备和观察实验
Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Compe
Raman Spectroscopy for the Detection of AGEs/ALEs
Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic Cells
Analysis of Gene Expression Using cDNA Microarrays
Some Ethical, Social, and Legal Considerations of Xenotransplantation
巨噬细胞的分离与纯化实验方法
G418筛选实验常遇问题总结
Fluorescein labeling of proteins
FISH Banding Techniques
Blastocyst Culture for Deriving Human Embryonic Stem Cells
Assessing Cell Size and Cell Cycle Regulation in Cells with Altered TOR Activity
Modeling miRNA Regulation in Cancer Signaling Systems: miR-34a Regulation of the p53/Sirt1 Signaling
Aptamers Targeting RNA Molecules
Internal Standards in Differentiating Embryonic Stem Cells In Vitro
Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by Using Small Molecules
Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection
Isolation and Culture of Human Umbilical Vein Endothelial Cells
Nuclear Reprogramming by Cell Fusion
Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template
Isolation and Characterization of Human Prostate Stem/Progenitor Cells
DNA Microarray Studies of Hematopoietic Subpopulations
Analysis of Actin Assembly by In Vitro TIRF Microscopy
Detection of Adhesion Molecules by Immunohistochemistry on Human and Murine Tissue Sections
Identification of mRNA Bound to RNA Binding Proteins by Differential Display
Placental and Fetal Steroidogenesis
Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis
Strategies for the Development of Novel Taxol-Like Agents
Serial Orthotopic Transplantation of Epithelial Tumors in Single-Cell Suspension
Multiple Site-Directed Mutagenesis
Assessing PCP in the Cochlea of Mammalian Ciliopathy Models
蛋白质寻靶(protein targeting)
High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophi
染色体实验技术分析
Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells
Cryopreservation of Shoot-Tips and Meristems
Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells
Generation and Use of Epitope-Tagged Receptors
Cystic Fibrosis Airway Epithelial Cell Culture
Characterization of B-Cell Maturation in the Peripheral Immune System
Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET
Evaluating the Localization and DNA Binding Complexity of Histones in Mature Sperm
Slice Cultures for Study of Microglia
Production of Transgenic Mice with Yeast Artificial Chromosomes
Methods to Monitor Fatty Acid Transport Proceeding Through Vectorial Acylation
Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products
Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors
核仁(nucleolus)
Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures
Structural Probing Techniques on Natural Aptamers
Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and
CCK8实验技巧与改进方案
CCK8实验目的
CCK8实验数据分析
CCK8实验新手教程
CCK8实验在细胞增殖研究中的应用与优势
CCK8实验的图都有哪些？
cck8实验注意事项
CCK8实验的关键步骤与技巧
CCK8实验的过程中的问题剖析和解决方案
CCK-8实验公式
冰冻切片实验原理以及应用
冰冻切片实验步骤
冰冻切片实验结果分析是怎么样的？
冰冻切片实验目的
冰冻切片实验技巧分享
常用的冰冻切片实验仪器有哪些？
冰冻切片和石蜡切片的区别是什么？
冰冻切片实验常见问题以及解决方法
冰冻切片实验注意事项
什么是lncRNA？
lncRNA实验步骤
lncRNA引物设计的方法
如何根据基因检测lncRNA？
lncRNA实验教程
lncRNA实验意义
LncRNA简介以及应用
lncRNA技术分类
透射电镜的结构与成像原理探究
透射电镜实验样品制备技巧
透射电镜观察什么？
如何分析透射电镜实验数据？
Conjugation of Ligands to the Surface of Preformed Liposomes by Click Chemistry
Isolation and Culture of Pig Epiblast Stem Cells
停止转移肽(stop-transfer peptide)
光学显微镜的使用与细胞化学成分的分析
Analysis of Proteins by Capillary Electrophoresis
运输ATPase(transport ATPase)
Transmission and Scanning Electron Microscope Preparation of Whole Cultured Cells
促细胞分裂剂激活单核细胞
渗透(osmosis)
Hydrodynamic Analysis of Human Kinetochore Complexes During Mitosis
Studying Cell Cycle Checkpoints Using Drosophila Cultured Cells
Collagen Degradation Assays
Growing cells
NMR Studies of Three-Dimensional Structure and Positioning of CPPs in Membrane Model Systems
有丝分裂(mitosis)
Analysis of the Mammalian Cell Cycle by Flow Cytometry
DNA-Protein In Situ Covisualization for Chromosome Analysis
小麦与蚕豆的小孢子形成
Size-Selective and In Vitro Assessment of Inner Blood Retina Barrier Permeability
Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse
Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses
Identification and Separation of Normal Hematopoietic Stem Cells and Leukemia Stem Cells from Patien
Regulation of Focal Adhesion Dynamics by Wnt5a Signaling
Comparison of CPP Uptake Methods
人白细胞介素11抗体（IL-11 Ab）酶联免疫分析（ELISA）
Purification and Activation of Cyclic GMP-Dependent Protein Kinase
Crystallization of Membrane Proteins for X-Ray Analysis
人酶联免疫分析（ELISA）
Nature methods发布2013最受关注技术成果：单细胞测序
Analysis of Tissue-Specific DNA Methylation During Development
Isolation and Differentiation of Hair Follicle-Derived Dermal Precursors
Murine Aggregation Chimeras and Wholemount Imaging in Airway Stem Cell Biology
Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol
Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization
Culture of Human Pancreatic Islet Cells
通过优化由软件控制的加、减速率离心分离单核细胞
Human Embryonic Stem (ES) Cell Protocols――Matrigel Aliquoting and Plating
Conditional Gene Mutagenesis in B-Lineage Cells
Lentivirus-Mediated Modification of Pluripotent Stem Cells
Immobilization of Photosynthetic Membranes in an Albumin-Glutaraldehyde Crosslinked Matrix
Cryopreservation of Algae
Gene Targeting in Human Pluripotent Stem Cells
Analysis of Embryonic Vascular Morphogenesis
Assay of Protein Kinases and Protein Phosphorylation
The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Pr
The Isolation of Membrane Proteoglycans
Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases
Profiling Stem Cells Using Quantitative PCR Protein Assays
Evolution of the Wnt Pathways
Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent In Situ Hybridization
Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
Methods to Analyze Cellular Necroptosis
2,2,6,6-Tetramethylpiperidin-1-Oxyl Probes for Evaluating Oxidative Stress on the Cell Membrane and
Efficient Hepatic Differentiation from Human iPS Cells by Gene Transfer
Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg
Detection of Apoptosis in Mammalian Development
Differentiate ES cells into cystic embryoid bodies
Derivation of Human Parthenogenetic Stem Cell Lines
RAPD-PCR with Parasitic Hymenoptera
Screening for Radiation Sensitizers of Drosophila Checkpoint Mutants
Immobilization of Cells in Polyelectrolyte Complexes
Isolation of Fetal Gonads from Embryos of Timed-Pregnant Mice for Morphological and Molecular Studie
Identification of Osteoclasts in Culture
Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and
Glycol Methacrylate Embedding for Improved Morphological, Morphometrical, and Immunohistochemical In
Redox Regulation and Trapping Sulphenic Acid in the Peroxide Sensitive Human Mitochondrial Branched
Immune Properties of Mesenchymal Stem Cells
猴子白细胞介素12（IL-12）酶联免疫分析（ELISA）
透射电镜是如何实现超高分辨率的呢？
透射电镜与扫描电镜的区别
扫描电镜和透射电镜样品的要求有什么区别？
如何选择透射电镜实验设备？
透射电镜在生物技术中的应用
透射电镜常见问题解答
免疫荧光染色技术原理
免疫荧光染色步骤
免疫荧光染色实验方法
免疫荧光染色实验所需材料
免疫荧光染色封片技巧
免疫荧光染色到底有哪些目的呢？
免疫荧光染色与免疫组化的比较
免疫荧光染色与流式细胞仪的比较
免疫荧光染色实验仪器
免疫荧光染色常见问题以及解决方法
基因表达调控的基本原理
基因表达调控基本特点
如何学习基因表达调控？
基因表达调控方式有哪些？
基因表达受哪些因素调控？
基因表达调控在生物制药中的作用
转录因子与基因表达调控
基因表达正调控与负调控
基因表达调控技术有哪些？
基因表达调控教程
巨噬细胞分型基础
巨噬细胞及其多功能性详解
巨噬细胞分型m1m2区别
巨噬细胞分型研究方法
巨噬细胞系有哪些？
Topology Determination of Untagged Membrane Proteins
Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae
Identification and Quantitation of Ketamine in Biological Matrices Using Gas Chromatography-Mass Spe
Gastrulation and Early Mesodermal Patterning in Vertebrates
Monitoring MicroRNA Expression During Embryonic Stem-Cell Differentiation Using Quantitative Real-Ti
Corneal Assay for Angiogenesis
Selection of pH Ranges in 2DE
Transgenic Manipulation of the Sea Urchin Embryo
In Vitro Methods to Culture Primary Human Breast Epithelial Cells
Combined Immunofluorescence, RNA Fluorescent In Situ Hybridization, and DNA Fluorescent In Situ Hybr
Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks
Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture
Producing Soluble Recombinant RNases and Assays to Measure Their Interaction with Interferon- In Vit
兼性异染色质(facultative heterochromatin)
人鳞状上皮细胞癌抗原（SCCAg）酶联免疫分析（ELISA）
Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral Transduction
Epigenome Analysis of Pluripotent Stem Cells
细胞自发转化的若干问题与解决方案
Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation
Immunolabeling of Embryos
Coupling of Nuclear Localization Signals to Plasmid DNA
Ectopic Expression in Drosophila
Derivation and Maintenance of Embryonic Stem Cell Cultures
Use of a Hemacytometer
Derivation and Characterization of Neural Cells From Embryonic Stem Cells Using Nestin Enhancer
细胞培养基无机离子的作用
Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis
Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length
Determination of the Endothelial Barrier Function In Vitro
Introduction to Mass Spectrometry
Reporter Gene Technologies for Imaging Cell Fates in Hematopoiesis
Expression from DNA Injected into Xenopus Embryos
Crystal Violet Assay
Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems
Isolation and Purification of Rabbit Osteoclasts
Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Ba
Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery
Platelet Life Span and Apoptosis
Human Embryonic Stem (ES) Cell Protocols――Embryonic Bodies
Expression and Function of Claudins in Hepatocytes
High-Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes
巨噬细胞分型与免疫治疗
巨噬细胞分型意义
巨噬细胞分型的科研应用
RNA免疫沉淀（RIP）实验原理
RNA免疫沉淀（RIP）实验步骤流程详解
RNA免疫沉淀（RIP）实验数据分析
RNA免疫沉淀（RIP）实验材料的准备
RNA免疫沉淀（RIP）实验在生物学中的应用
RIP实验和pulldown的区别
RNA免疫沉淀（RIP）实验常见问题解答
RNA免疫沉淀（RIP）实验高级技巧
如何优化染色质免疫共沉淀（ChIP）实验条件？
ChIP实验常见问题解答
铁死亡
确保ChIP实验测序结果准确性的关键因素有哪些？
解释ChIP数据解读中需要注意的关键点
染色质免疫共沉淀（CHIP）实验原理详解
什么是ELISA实验？
ELISA实验步骤详解
ELISA实验数据分析与解读
Epithelial Cell Migration in Response to Epidermal Growth Factor
Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions
Exo Ovo Culture of Avian Embryos
Extraction of RNA from Fresh and Frozen Blood
Detection of Herpes Simplex Virus Dependent Apoptosis
The Design and Application of Ribosomal RNA-Targeted, Fluorescent Oligonucleotide Probes for the Ide
Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology
Biochemical Analyses of the Electron Transport Chain Complexes by Spectrophotometry
Flow Cytometric Analysis of Cell Cycle
Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functio
Analysis of Ligand-Dependent Nuclear Accumulation of Smads in TGF- Signaling
Human Embryonic Stem Cell Derivation, Maintenance, and Differentiation to Trophoblast
Overview: Assays for Studying Integrin-Dependent Cell Adhesion
Extraction and Assay of Cyclic Nucleotides
ELISA实验材料
引发体(primosome)
Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells
ELISA的实验报告（一）
In Vivo Imaging of Hematopoietic Stem Cells in the Bone Marrow Niche
Measurement of Mitochondrial dNTP Pools
ELISA检测实验报告（二）
Capabilities and Drawbacks of Phospholipid Analysis by MALDI-TOF Mass Spectrometry
The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability
Methods to Evaluate Alterations in Polyamine Metabolism Caused by Helicobacter pylori Infection
ELISA实验在生物医学研究中的应用案例
Experimental Approaches to the Study of Human Primordial Germ Cells
Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval
快速解读ELISA实验结果
Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells
ELISA实验中的创新技术——加速科研进程的新工具
ELISA试剂盒选择与使用指南
ELISA实验与Western Blot的异同点解析：如何选择更适合实验方法？
ELISA实验注意事项
ELISA实验常见问题解答
shRNA序列设计基因干扰原理
shRNA序列设计的具体步骤是什么？
探讨减少shRNA序列脱靶效应的方法
‌shRNA序列设计的关键因素
shRNA序列设计实战技巧：优化基因表达调控
介绍一些常用的shRNA表达载体
最新shRNA序列设计趋势与策略
构建shRNA序列设计流程
阐述siRNA和shRNA在细胞实验中的使用差异
分析siRNA和shRNA在细胞实验中可能遇到的问题及相应的解决方案
siRNA、shRNA 都是啥？
介绍提高shRNA转染效率的具体方法
解锁shRNA序列设计的秘密【提升实验成功率】
琼脂糖凝胶电泳步骤
提供预防琼脂糖凝胶中核酸降解的方法
琼脂糖凝胶电泳的上样量是多少？
Utility of Animal Models for Human Embryo Culture: Nonhuman Primates
琼脂糖凝胶电泳原理
Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs
Experimental Control and Characterization of Autophagy in Drosophila
COPⅠ被膜小泡(COPⅠcoated vesicle)
Homeoprotein Intercellular Transfer, the Hidden Face of Cell-Penetrating Peptides
关于琼脂糖凝胶电泳未出现条带的原因
Propagation of Human Embryonic Stem Cells on Human Feeder Cells
Methods for Imaging Cell Fates in Hematopoiesis
Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice
Multi-Dimensional Capillary Electrophoresis and Chromatography for Proteomic Analysis
Telomerase Inhibition by Synthetic Nucleic Acids and Chemosensitization in Human Bladder Cancer Cell
给出优化‌琼脂糖凝胶电泳结果分析‌的方法
The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions
探讨琼脂糖凝胶电泳在分子生物学研究中的发展趋势
Primary Human Osteoblast Cultures
细胞工程技术(cell engineering)
Silencing of Rho-GDI by RNAi Promotes the Differentiation of Neural Stem Cells
Induction of Cellular Senescence by Oncogenic RAS
Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer
In Vivo Matrigel Migration and Angiogenesis Assay
Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour of Cancer Cells In Vivo
The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery
Microinjection into Zebrafish Embryos
Descriptive Statistics
Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System
核孔复合物(nuclear pore complexs,NPCs)
Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry
Identification of Clock Genes Using Difference Gel Electrophoresis
Optimizing Stem Cell Grafting into the CNS
Analyzing Double-Strand Repair Events in Drosophila
微管(microtubule)
Novel Designed Probes for the Characterization of Oxidative Stress in Biological Fluids, Cells, and
High-Resolution Fluorescence In Situ Hybridization to Detect mRNAs in Neuronal Compartments In Vitro
Restriction Endonucleases and Their Uses
琼脂糖凝胶电泳试剂
琼脂糖凝胶电泳注意事项
亚细胞定位实验是什么？
亚细胞定位分析
详细描述GFP融合蛋白表达法的实验过程
亚细胞定位实验步骤
详细描述亚细胞定位实验的数据处理方式
列举几个影响亚细胞定位实验结果的关键因素
亚细胞定位实验结果解读
探讨如何优化亚细胞定位实验的设计以提高结果的准确性
对比分析不同亚细胞定位实验技术的优缺点
亚细胞定位实验设备
列举并分析亚细胞定位实验中的常见问题以及解决方案
巨噬细胞原代提取
‌巨噬细胞原代培养实验报告‌
巨噬细胞原代培养前后的区别
巨噬细胞m1m2标志物
详细描述巨噬细胞原代培养中细胞分离与培养的具体步骤
描述巨噬细胞原代培养中细胞贴壁的过程
探讨巨噬细胞原代培养中如何优化细胞活性
coip实验原理
coip实验流程以及步骤
coip实验设计
COIP实验步骤中，去除杂蛋白的具体方法是什么？
实验过程中，如何确保样品的低温处理？
coip实验数据解读
coip实验目的
coip实验后，如何分析实验结果?
分析CO-IP结果时，有哪些常见的误区需要避免？
如何优化CoIP实验以提高其准确性？
coip实验材料
‌coip实验应用
在设计COIP实验时，如何选择合适的抗体？
在进行COIP实验时，有哪些常见的问题和解决方法
CHIP引物设计原则
蛋白质翻译后修饰
RNA干扰技术
增强子及超级增强子序列查找
原核表达载体和真核表达系统及其载体
分子生物学导论
ChIP实验中引物序列的选择策略
在CHIP实验中，如何确保引物的特异性和有效性？
CHIP引物设计步骤
CHIP引物设计技巧
CHIP引物设计与基因表达的研究
CHIP实验引物要求‌
CHIP引物设计结果分析
在设计CHIP引物时，如何确保引物的扩增效率？
CHIP引物设计常见问题
CHIP引物设计教程
Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in
Quantitation of 17-OH-Progesterone (OHPG) for Diagnosis of Congenital Adrenal Hyperplasia (CAH)
Detection of Aromatic -Hydroxyketones with Tetrazolium Salts
Random Mutagenesis of Short Target DNA Sequences via PCR with Degenerate Oligonucleotides
Analysis of Nuclear UracilDNA Glycosylase (nUDG) Turnover During the Cell Cycle
Detection of Cell Death in Drosophila
Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpole
Adult Stem Cells for the Treatment of Neurological Disease
Long-Circulating, pH-Sensitive Liposomes
Detection of Nuclear -catenin in Xenopus Embryos
Zymographical Techniques for Detectionof Matrix Metalloproteinases
Aging Studies in Drosophila Melanogaster
The Mitochondria of Cultured Mammalian Cells: I: Analysis by Immunofluorescence Microscopy, Histoche
Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies
Use of Divalent Metal Ions Chelated to Agarose Derivatives for Reversible Immobilization of Proteins
Analysis of EGF Receptor Interactions With the Subunit of the Stimulatory GTP Binding Protein of Ade
Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of
Analysing the ATP Turnover Cycle of Microtubule Motors
Isolation of mRNAs Encoding Peroxisomal Proteins from Yeast Using a Combined Cell Fractionation and
Isolation, Characterization, and Reaggregate Culture of Thymic Epithelial Cells
Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo
Application of Cre/loxP in Drosophila: Site-Specific Recombination and Transgene Coplacement
Isolation and Culture of Adult Mouse Hepatocytes
Expression of Recombinant ADAMTS in Insect Cells
Analysis of Microtubule Assembly Kinetics Using Turbidimetry
Hematopoietic Progenitor Cell Collection
Direct Sequencing with Highly Degenerate and lnosine-Containing Primers
Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities
培养物的污染及防止
The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its
Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell T
Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress
体外肝细胞损伤模型建立（CCl4和H2O2）
Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells
In Vitro Assays for Neural Stem Cell Differentiation
Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitt
A Transplant Model for Human Epidermal Skin Regeneration
Real-Time RT-PCR for CTG Repeat-Containing Genes
Isolation of Total RNA from Bacteria
The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells
支原体污染后的治疗和处理
Quantitating mRNAs with Relative and Competitive RT-PCR
Reverse Transfected Cell Microarrays in Infectious Disease Research
Copy Number Determination of Genetically-Modified Hematopoietic Stem Cells
High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays
CHIP引物设计四个注意事项详细点
细胞铁死亡的定义
细胞铁死亡的机制
细胞铁死亡造模流程
细胞铁死亡信号通路
细胞铁死亡金标准
细胞铁死亡研究与应用整合
细胞铁死亡实验方法
细胞铁死亡和凋亡的区别
入门细胞铁死亡研究，这些基础知识你都知道吗?
非编码RNA作用原理是什么？
非编码RNA定义
非编码RNA在细胞分裂中有什么作用？
非编码RNA种类和功能
长非编码RNA如何参与基因转录调控？
非编码RNA研究的主要方向是什么？
非编码RNA和编码RNA区别
非编码RNA由什么转录？
Routine Culture and Differentiation of Human Embryonic Stem Cells
An Immunoprecipitation-RNA:rPCR Method for the In Vivo Isolation of Ribonucleoprotein Complexes
非编码RNA的实验室检测技术
Under-Agarose Chemotaxis of Dictyostelium discoideum
加州大学研究人员开发出了“智能培养基
Genetic-Engineered Models of Skeletal Diseases I: Collagen Type X
非编码RNA特点
Analysis by HPLC of Distributive Activities and the Synthetic (Back) Reaction of Pancreatic-Type Rib
Tissue Culture - Thawing Cells from Liquid Nitrogen
细胞自噬大盘点
In Vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth Characteristics
Keratinocyte Culture
细胞自噬机制
BacterialBacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins
Optimized Neuronal Differentiation of Murine Embryonic Stem Cells: Role of Cell Density
细胞自噬与肿瘤的关系
RC4DRestriction Fragment Length Polymorphism-Coupled Domain-Directed Differential Display
Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue
Senescence Regulation by mTOR
GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications
细胞自噬与免疫调节
Analyzing the Angiogenic Potential of Gr-1+CD11b+ Immature Myeloid Cells from Murine Wounds
Optimizing Sporulation Conditions for Different Saccharomyces cerevisiae Strain Backgrounds
细胞自噬与衰老
The Use of Synthetic DNA Probes for the Field Identification of Members of the Anopheles gambiae Com
细胞自噬：从基础到临床，生命科学的深度解析
Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS
Electrofusion of Mouse Embryos to Produce Tetraploids
Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development
多线染色体(polytene chromosome)
Immunofluorescence of Cultured Cells
DNA Damage Response and Mutagenesis in Mouse Embryonic Stem Cells
Tissue Procurement for Molecular Studies Using Laser-Assisted Microdissection
Pharmacology of Antisense Therapeutic Agents: Cancer and Inflamination
Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach
Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Folli
Structural Studies of MAP Kinase Cascade Components
RACE实验是什么？【分子生物学】
Preparation of DRV Liposomes
Procedures to Evaluate the Importance of Dietary Polyamines
RACE实验提高扩增特异性和效率的新方法
RACE实验与基因表达分析
cDNA末端快速扩增技术（RACE）在基因克隆中的实践
脊髓星形胶质细胞机械性损伤的体外培养模型
探索RACE实验在疾病相关基因研究中的应用价值
RACE实验优化策略：提升实验成功率的实用技巧
mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric In Situ Hybridizatio
RACE实验有哪些发展趋势？
Directing Human Embryonic Stem Cells to a Retinal Fate
Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests
Time-lapse Microscopy and Fluorescence Resonance Energy Transfer to Analyze the Dynamics and Interac
Cloning of Exotic/Endangered Species: Desert Bighorn Sheep
Cryo-electron Tomography of Microtubules Assembled In Vitro from Purified Components
TUNEL labeling
RACE实验在基因克隆中的应用：从理论到实践的跨越
Quantification of Protein Carbonylation
Plasmid DNA Purification Via the Use of a Dual Affinity Protein
RACE实验深度探索：从原理到应用的全面解析
Affinity-Based Assays for the Identification and Quantitative Evaluation of Noncovalent Poly(ADP-Rib
Regulation of MAP Kinase Signaling by Calcium
Chemokine Receptor Signaling and HIV Infection
模板组装(template assembly)
Development of Phosphorylation Site-Specific Antibodies to Nuclear Receptors
考马斯亮蓝染色原理
Sequence-Based Typing of HLA: An Improved Group-Specific Full-Length Gene Sequencing Approach
Overview of Cell Synchronization
考马斯亮蓝染色步骤全解析
Isolating mRNA and Small Noncoding RNAs from Human Sperm
Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs
考马斯亮蓝测蛋白浓度步骤
Single-Cell Transcript Profiling of Differentiating Embryonic Stem Cells
养大鼠平滑肌细胞的一点心得
考马斯亮蓝染色原理与传统染色
Viral Infection Protocols
与传统染色相比，考马斯亮蓝染色有哪些具体优势？
Purification of Drosophila Protein Complexes for Mass Spectrometry
Rhythmic Conidiation in Neurospora crassa
考马斯亮蓝染色后，如何保存凝胶？
Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic
Measuring the Formation and Repair of UV Photoproducts by Ligation-Mediated PCR
如何正确进行考马斯亮蓝染色？
Transmitochondrial Cybrids: Tools for Functional Studies of Mutant Mitochondria
Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediat
细胞凋亡与细胞坏死的的区别
Separation and Maintenance of Primary T and B Lymphocytes
The Extraction and Affinity Chromatography of Messenger RNA
考马斯亮蓝染色常见问题解析
Methods for Culturing Mouse and Human Embryonic Stem Cells
A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of S
Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and C
Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems
考马斯亮蓝染色液配方轻松掌握实验技巧
Quantification of Cell Cycle-Arresting Proteins
The Secret Lives of Dictyostelium
考马斯亮蓝染色注意事项
TSA多色免疫荧光原理介绍
TSA多色免疫荧光技术教程
TSA多色免疫荧光结果解读
TSA多色免疫荧光试剂推荐
TSA多色免疫荧光与单色免疫荧光的区别
TSA多色免疫荧光染色方法，轻松掌握多色染色技巧
TSA多色免疫荧光在科研中的应用《科研探索中的新利器》
动物体内转染试剂的作用原理是什么？
首都医科大学报道：治疗对乙酰氨基酚过量造成肝损伤的新策略
动物实验系列篇章之七—— 实验动物的处死
英格恩生物和您一起探究靶向LunX抑制肺癌的生长及转移
同济大学医学院郭传勇教授团队报道：非酒精脂肪肝新疗法
中国医科大学报道：谁在保护我们的脊椎神经？
转基因动物的那些事儿，想说你为什么这么难？
动物体内转染实验的重复性如何？
西安交通大学附属医院：促进心肌损伤的杀手
四川大学口腔医院：一种诱导口腔鳞状细胞癌恶化的信号通路
中国药科大学齐炼文教授团队报道：治疗II型糖尿病的新策略
什么是动物实验中提倡的“3Rs”概念？
动物体内转染技术可以做什么？
Theranostics | 复旦大学儿童医院报道：揭开I型糖尿病的发病机制
基因敲除实验和动物体内转染实验（entranster-invivo）对比
动物实验系列篇章之三——实验动物的麻醉方法
动物体内转染注射技巧
动物体内转染实验的可靠性如何？
南京医科大学孙秀兰教授团队报道：缺血性中风的新型治疗策略
海军医科大学王文忠教授团队报道：改善高血压氧化应激的新策略
郑州大学附属医院：卵巢癌患者或将迎来新的希望
实验动物的遗传学分类——突变系动物
影响实验动物的环境因素有哪些？
厦门大学医学院：如何才能有效缓解神经炎症疼痛？
如何快速实现蝗虫、七鳃鳗、猪等（非小鼠）动物的体内基因敲除/表达？
转基因动物和嵌合体动物
西北农林科技大学报道：如何有效增肌?
实验动物的遗传学分类——近交系动物
软骨肉瘤治疗研究新进展
动物体内转染常见问题与解决方案
EntransterTM-in vivo体内转染和病毒体内转染的比较
如何改善肾间质纤维，防止肾衰老？
动物体内转染试剂entranster-invivo的主要特点
动物实验系列篇章之六——实验动物各种体液、骨髓的采集方法
动物体内转染后，遗传性状能维持多久？
动物实验系列篇章之二——实验动物的编号和分组
动物体内转染实验，如何评估不同组织的干扰效率？
一个抑制脑中风的miRNA
动物体内转染试剂可以进行哪些研究？
动物实验系列篇章之四——实验动物的除毛、给药方法
小鼠体内转染实验，用滴鼻的方式，如何观察液体在肺部的分布
动物体内转染，局部注射后应如何取样？
动物体内转染实验给药剂量
动物实验系列篇章之五——实验动物的采血法
做大鼠慢性肾衰竭（CRF）模型
小鼠尾静脉注射技巧有哪些？
实验动物的遗传学分类——系统杂交动物
Neuropharmacology | 中国药科大学姚文兵教授团队报道：治疗阿尔兹海默症的新视角
免疫组化实验结果的判定原则和分析标准
免疫组化技术大全
石蜡切片制作（苏木精-伊红对染法）详细步骤
包埋剂的配制及注意事项
石蜡包埋法如何选择石蜡？
病毒包装过程中，细胞大量死亡，还有必要收集病毒吗
免疫共沉淀经验总结分享
慢病毒感染，胶体金测出来很高，可感染效果很差的原因
电镜包埋剂应该具备哪些特征？
免疫组化的经验总结－常见问题
亲和层析纯化抗体,请问纯化后收集到的抗体溶液，加甘油或者醇类，糖类的话，浓度一般是加多少
免疫共沉淀实验原理及注意事项
JAM分析
培养基中pH 的调节
细菌生长曲线的测定及其意义
通过MTT 法分析细胞活力
siRNA转染实验的几点建议
细胞siRNA转染有哪些方法？
如何制备含血清的培养基？
mRNA细胞转染实验步骤
细胞电转染实验效率低的原因是什么？
Hela细胞的电转染条件是什么？
细胞培养类型的分类：根据生长习性分类
单层细胞的培养
siRNA与DNA转染有什么不同？
细胞电转染实验效率不理想的原因
细胞电转染实验注意事项
DEAE－葡聚糖介导的转染：提高细胞活力的方案
如何构建siRNA表达载体？
如何达到更好的siRNA转染效率？
细胞转染的方法有哪些？
RNA转染效率影响因素
细胞转染实验的建议
肿瘤细胞的原代培养
实验室小白篇：细胞培养的基本操作
病毒感染的细胞与未感染的对照细胞相比，在相同浓度的嘌呤霉素（puromycin）处理下，病毒感染的细胞死亡更多的原因
siRNA转染效率低的原因有哪些？
如何选择正确的方法进行细胞的支原体检测？
细胞RNA转染实验注意事项
慢病毒感染后，细胞不生长怎么办
细胞转染实验易出现的问题
纳米材料转染试剂（Entranster）优势研究
细胞转染效率重复性差的原因是什么？
常用的细胞株
如何上调或下调特定的miRNA？
电转染实验，如何设置正确的siRNA实验对照组？
细胞转染实验方法有哪些？
细胞DNA转染效率影响因素
影响RNA转染的因素
病毒感染细胞实验整体流程
如何判断细胞是否被污染？
细胞的冷冻和储存
悬浮细胞的分瓶操作步骤
siRNA转染后出现细胞死亡是什么原因？
磷酸钙-DNA共沉淀法的原理和步骤
脂质体细胞转染试剂的缺点
细胞转染实验注意事项
Jurkat E6-1细胞的电转染条件是什么？
细胞转染实验中易出现的问题
怎么样做好siRNA细胞转染实验？
体外培养细胞的一般性质（二）
转化细胞在软琼脂中的生长
实验室小白篇：细胞培养用品及其准备
电转效率受哪些因素影响？
如何能达到较高的细胞转染效率？
改良的Boyden 小室法观察细胞的的运动性
提高RNA转染效率的几点建议
慢病毒感染后，未能实现相关敲降的原因是什么？
实验室小白篇：细胞培养用的液体
RNA转染过程中细胞毒性大怎么办？
人胚胎肺成纤维细胞的培养
慢病毒感染细胞后，细胞长满48小时是否可以先传代，再等到72小时再喷霉素筛选吗
电转染过程中易出现的问题
细胞的支原体检测——扫描电子显微镜法
贴壁细胞的分瓶操作步骤
细胞电转染实验的几点建议
为什么脂质体细胞转染试剂毒性大？
监测慢病毒载体储备液中的可复制型病毒
RNA细胞转染过程注意事项
实验中最常见的英文缩写词汇释义
人胚眙肝细胞的培养
如何能够做好siRNA转染实验？
影响RNA转染实验的因素有哪些？
PBMC细胞可以用电转染吗
慢病毒转染贴壁细胞实验方法
成功转染siRNA的主要关键点有什么？
病毒包装过程中，细胞大量死亡，还有必要收集病毒吗
细胞转染效率低的原因及解决方案
siRNA细胞转染应注意的事项
如何优化RNA转染条件？
细胞的聚集体培养
单层细胞如何用胰蛋白酶处理？
都说RNAi很容易降解，转染效率很低，是真的吗？
做好RNA转染的几点建议
shRNA转染实验步骤
如何解决病毒感染细胞效率低的难题？
细胞培养该不该加抗生素?
siRNA转染常见问题及解决方案
细胞生长速度非常快，现在在密度20-30%时就开始转染，但是到了60小时后细胞密度已经非常高，几乎没有空隙。如果继续等待，是否会导致细胞状态不佳，从而影响到实验中蛋白的表达效果。
质粒DNA转染实验效率影响因素
请问共转染的两种质粒需要选择不同载体吗
质粒DNA转染注意事项
慢病毒感染贴壁细胞实验方法
哪种RNA转染试剂效果更好？ Turbofect or EntransterTM-R4000？
免疫细胞的制备方法和注意事项
如何选择稳定转染的筛选抗生素？
慢病毒感染12孔板种植多少细胞合适
体外培养细胞的一般性质（四）
电转染实验效率影响因素
做好siRNA转染的要点
Abnbio ABP31082 特级胎牛血清盒装血清 FBS（10*50mL）
电转染实验时，细胞密度是多少合适？
慢病毒感染，胶体金测出来很高，可感染效果很差的原因
细胞计数的方法
siRNA细胞转染操作步骤
细胞RNA转染实验的一些建议
Abnbio ABF106 标准胎牛血清 FBS 500ml
细胞转染注意事项
如何利用好慢病毒感染的原理和特点？
细胞转染实验效率影响因素
RNA转染与人鼻病毒1B基因敲除和干扰素应答的再激活研究
如何选择哺乳动物高效表达载体
电转染实验的建议
在慢病毒感染细胞的实验中，感染后细胞状态看起来正常，但传代培养后细胞死亡较多的原因
如何观察细胞？
细胞转染siRNA后，目标基因的表达反而升高的原因是什么？
提高病毒感染细胞实验效率的方法
细胞转染的实验方法都有什么？
Abnbio AB30042 0.25%胰酶细胞消化液含EDTA含酚红 100ml
细胞复苏的步骤
BHK-21细胞的电转染条件是什么？
RNA转染效率低的原因是什么？
shRNA的质粒转录水平敲下来了百分之90的解决办法
Abnbio AB30010 双抗青链霉素混合液(100×)细胞培养专用 100ml
siRNA转染实验的关键点
RNA转染（entranster）后沉默现象不明显怎么办？
视神经星状胶质细胞的培养
siRNA细胞转染实验步骤
siRNA答疑专题总结（上）
Abnbio DMEM低糖培养基含L-谷氨酰胺丙酮酸钠 A118855 500ml
细胞RNA转染的建议
不同类型细胞的生长形态
荧光表达的细胞在冻存后72小时后再测荧光情况，可以吗?
siRNA转染实验注意事项
将siRNA转染入贴壁动物细胞的转染方案及相关检测
兔胸主动脉平滑肌细胞的原代培养
慢病毒感染，铺板后24小时感染，病毒量是不是按铺板时细胞数的二倍计算的？
Abnbio DMEM/F12培养基含L-谷氨酰胺 HEPES 丙酮酸钠 A113305 500ml
细胞DNA转染注意事项
双质粒表达系统，如何选择启动子?
细胞转染效率影响因素
Abnbio RPMI1640培养基含L-谷氨酰胺不含HEPES A118755 500ml
Abnbio DMEM高糖培养基含L-谷氨酰胺丙酮酸钠 A119955 500ml
为什么我的细胞转染实验效率较低？
为什么脂质体细胞转染试剂会造成细胞毒性？
RNA细胞转染实验影响因素
如何达到较高的RNA转染效率？
细胞铺板密度对于siRNA转染为什么很重要？
绿原酸的结构特征及配置
醋酸钠的性质及用途
关于四季青优级胎牛血清相关介绍
硫酸钙的性质及应用
马铃薯淀粉的作用及培养基的配置
脱落酸的作用以及需要注意事项
LB培养基和MS培养基的比较与区别
凝血酶的作用
蛋白酶k配制、使用方法及作用
甲苯与纯苯的价差收窄暗示了未来甲苯调整的可能性
茜素红染色和硝酸银法，有什么区别。
siRNA转染至细胞有哪些方法？
对于难转染的细胞，如何提高其转染效率？
慢病毒滴度的计算
siRNA是从牵牛花过表达发现的吗
哺乳动物细胞同时表达2个质粒，如果采用相同的启动子，会不会形成表达竞争
如何做好细胞电转染实验？
细胞转染应该什么时候加抗生素？
哪些因素会影响细胞转染实验的效率？
为什么entranster试剂可以进行有血清细胞转染？
悬浮细胞的siRNA转染实验步骤
细胞培养的无菌技术
血清对于细胞转染效率有什么影响？
细胞转染实验的不同方法
提高细胞转染效率的要点
体外培养细胞的一般性质（一）
电转染实验时，DNA有什么要求？
siRNA转染效率不理想的原因
细胞电转后，72小时铺板加药筛选是否可行
病毒感染细胞效率低怎么办？
细胞冻存的7个注意事项
怎样做好细胞电转染实验？
电转染实验中易出现的问题
siRNA细胞转染过程中常见问题及解答
双质粒共转染哺乳动物细胞
如何做好DNA转染实验？
为什么我的细胞转染效率低？
怎样提高悬浮细胞的siRNA转染效率？
如何选择慢病毒和腺病毒系统？
关于细胞培养基你需要知道的那些事
电转染实验影响因素
RNA转染过程中常见问题及解决方案
siRNA转染效率不佳的原因
miRNA对划痕愈合（细胞迁移）的影响
使用血清时要注意哪些参数？
RNA转染影响因素
细胞转染需要注意什么事项
siRNA转染实验步骤
细胞培养常用的抗生素有哪些？
细胞转染实验影响因素
电转染时，指数衰减脉冲形式的电转条件是什么？
影响细胞转染效率的因素
细胞转染效率的影响因素
怎样做好siRNA细胞转染实验
如何制备血清减量或无血清培养基？
细胞转染实验的方法都有什么？
细胞周期测定实验有哪些，怎么做?
如何分离成纤维细胞？
siRNA转染后如何在蛋白水平敲低
高滴度反转录病毒和慢病毒载体的制备
慢病毒感染后72小时，效率低，细胞已经长满，有什么办法补救？
Behavioral Methods for the Study of the RasERK Pathway in Memory Formation and Consolidation: Passiv
Bone Marrow-Derived Mesenchymal Progenitor Cells
Skeletal Morphogenesis
Function of the Wingless Signaling Pathway in Drosophila
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: DNase I Digestion
Retroviral and Lentiviral Vector Titration by the Analysis of the Activity of Viral Reverse Transcri
Developmental Genetics in Xenopus tropicalis
Using 3D Culture to Investigate the Role of Mechanical Signaling in Keratinocyte Stem Cells
Analysis of Replicating Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis
Methods for Preparing Crystals of Reversibly Oxidized Proteins: Crystallization of Protein Tyrosine
Use of Simian Immunodeficiency Virus Vectors for Simian Embryonic Stem Cells
Methods to Analyze Subcellular Localization and Intracellular Trafficking of Claudin-16
Directed Differentiation of Human Pluripotent Stem Cells Along the Pancreatic Endocrine Lineage
Statistical Analysis of Arbitrarily Primed PCR Patterns in Molecular Taxonomic Studies
With the development of the technique of autoradiography by Howard and Pelc (16 ), the mammalian cel
Cell Synchrony Techniques: A Comparison of Methods
Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structur
Gene Dosage in Capillary Electrophoresis: Prenatal Diagnosis of Downs Syndrome and Rh D/d Genotyping
Highly Efficient Directed Differentiation of Human Induced Pluripotent Stem Cells into Cardiomyocyte
Chorioallantoic Membrane (CAM) Assay
转决定(transdetermination)
Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA
B-Cell Development and Pre-B-1 Cell Plasticity In Vitro
Assessment and Quantification of Telomerase Enzyme Activity
Functional Screening with a Live Cell Imaging-Based Random Cell Migration Assay
动物细胞微丝束的光学显微镜观察
细胞器的光镜切片和电镜照片观察实验
Assays of Pyruvate Dehydrogenase Complex and Pyruvate Carboxylase Activity
Culture of Human and Mouse Mesenchymal Cells
Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically D
Membrane Lipidomics and the Geometry of Unsaturated Fatty Acids From Biomimetic Models to Biological
大鼠活性氧簇（ROS）酶联免疫分析
M199培养基的正确使用方法
Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle fo
Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms
Are There Any Normal Clones
体节极性基因(segment-polarity genes)
Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay
Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization
Microvessel Endothelial Cells from Human Adipose Tissues: Isolation, Identification, and Culture
Detection of MicroRNAs in Prostate Cancer Cells by MicroRNA Array
Assay for the Isolation of Hepatogenic Factors: Key Molecules in Hepatocyte Formation and Liver Morp
A Dock and Lock Approach to Preparation of Targeted Liposomes
Logistic Regression
Studies on MAP Kinase Signaling in the Immune System
原代软骨细胞分离培养
Claudin Family Proteins in Caenorhabditis elegans
Mitochondrial Division in Caenorhabditis elegans
线粒体膜间隙(intermembrane space)
Generation of Paint Probes by Flow-Sorted and Microdissected Chromosomes
Electroporation of Antibodies into Mammalian Cells
Cryopreservation of Mammalian Embryos: Vitrification
The Use of FRET Microscopy to Elucidate Steady State Channel Conformational Rearrangements and G Pro
Fluorescence Resonance Energy Transfer to Study Receptor Dimerization in Living Cells
无血清培养基培养乳猪肝细胞的效果
Measurement of Wee Kinase Activity
Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of
Techniques for the Measurement of Nitric Oxide
细胞转染后怎么选择抗生素
粗线期(pachytene stage, pachynema)
End-Point Titration-PCR for Quantitation of Cytomegalovirus DNA
Pharmacology, Biodistribution, and Efficacy of GPCR-Based Pepducins in Disease Models
2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics
In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells
Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clo
Lipid Geographical Analysis of the Primate Macula by Imaging Mass Spectrometry
Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena
新型核酸分子荧光探针——分子信标的原理及应用
做无菌实验前，你需要做好这些准备
RNA沉淀方法
如何做好Northern Blot实验？
SDS-PAGE蛋白质样品的制备
质粒DNA细胞转染实验效率影响因素有哪些？
南京中医药大学：忍冬苷靶向EZH2减轻溃疡性结肠炎通过自噬介导NLRP3炎性体失活
双脱氧法和化学测序法如何选择？
单克隆抗体的制备——杂交瘤的建系
RNA提取的注意事项
丁醇抽提法浓缩核酸
PCR、RT-PCR、qRT-PCR实验精髓
如何将胶内物质转移到膜上？
DNA 序列测定——双脱氧( Sanger ) 测序法
蛋白质实验的注意事项
细胞融合与杂交瘤分选
反转录定量PCR 分析小RNA
DNA的乙醇沉淀
DNA 序列测定——化学(Maxam-Gilbert) 测序法
蛋白质的水平测定-含有去污剂的情况
shRNA转染后，什么时候做RT-PCR最好?
聚丙烯酰胺凝胶电泳注意事项
慢病毒感染，胶体金测出来很高，可感染效果很差的原因
Trizol法提取总RNA的原理及注意事项
荧光原位杂交会用到哪些试剂？
western-blot实验常见问题及解答
从革兰氏阴性菌（如E.coli）中分离DNA
分子克隆中常用的其他酶
体外转录法制备dsRNA
DNA分离前你应该知道的那些事
Western-blot化学发光(ECL)时，胶片上条带很弱是什么原因？
EMSA实验步骤
造成western-blot发光时发生荧光淬灭的因素有哪些？
实时荧光定量PCR为什么倍受青睐？
转子的类型及用途
慢病毒感染后，未能实现相关敲降的原因是什么？
降落PCR实验原理及步骤
测定蛋白质浓度前需要知道什么？
Northern Blot原理及所需仪器、试剂
RNA的提取方法及原理
PCR实验步骤及常见问题汇总
western-blot化学发光（ECL）的特异性差怎么办
昼夜节律转录因子BMAL1在生殖内分泌学和生育中的关键作用
western-blot化学发光（ECL）时，胶片上条带很浓怎么办？
动物基因组DNA的提取
如何提高PCR反应的特异性？
什么是反向PCR？
AFLP原理和操作步骤
荧光表达的细胞在冻存后72小时后再测荧光情况，可以吗?
双脱氧法DNA 测序常见问题
Quantification of Genetically Controlled Cell Death in Budding Yeast
The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures
细胞生物基本方法：特殊培养法
TUNEL PROCEDURE IN BOVINE EMBRYOS
Electrofusion of Mammalian Cells
Nuclear Reprogramming: An Overview
双线期(diplotene stage)
Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B and Clonogenic
Construction of Polycompetitors for Competitive PCR
A Rapid and Efficient Platelet Purification Protocol for Platelet Gene Expression Studies
Electrochemotherapy for the Treatment of Soft Tissue Sarcoma in a Rat Model
Analysis of ES Cell Clones by Mini-Southern
Organelle Proteomics
Riboswitch Conformations Revealed by Small-Angle X-Ray Scattering
Investigation of KATP Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blot
Hepatic Differentiation of Mesenchymal Stem Cells: In Vitro Strategies
质粒的不同对同一细胞的G418筛选浓度影响大吗
Analysis of Bone Architecture in Rodents Using Microcomputed Tomography
Yeast Mating: A Model System for Studying Cell and Nuclear Fusion
Patterning Mouse and Human Embryonic Stem Cells Using Micro-contact Printing
Analysis of Autophagosome Membrane Cycling by Fluorescence Microscopy
如何选择和使用细胞培养板
Recommended Experiments with Isolated Mitochondria
Isolation of Peptide Aptamers to Target Protein Function
High-Resolution Confocal Imaging in Tissue
Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipases C an
Laser Capture Microdissection for Analysis of Single Cells
Electrochemotherapy of Murine Melanoma Using Intratumor Drug Administration
Measurement of Adenylyl Cyclase Activity in Cell Membranes
Analyzing RNA-Protein Crosslinking Sites in Unlabeled Ribonucleoprotein Complexes by Mass Spectromet
ssessment of Thymidine Phosphorylase Function: Measurement of Plasma Thymidine (and Deoxyuridine) an
Morphological Assessment of Mitochondrial Respiratory Chain Function on Tissue Sections
Calcium Alginate Film Formed on a Stainless Steel Mesh
Noninvasive Measurement of Hematopoietic Stem Cell Cycling and Turnover by In Vivo Bromodeoxyuridine
胸腹水细胞染色体制备
Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (Milkweed Bug Oncopeltus f
外膜(outer membrane)
Insight into Cell-Entry Mechanisms of CPPs by Electron Microscopy
Comprehensive Quantitative Analysis of Bioactive Sphingolipids by High-Performance Liquid Chromatogr
Proteomic Analysis of Human Neutrophils
生物膜
如何检验细胞是否已经凋亡？
Application of Polymerase Chain Reaction for the Discovery of New Adhesion Molecule Family Members
Inhibition of Tumor Metastasis by Liposomes Containing Glyco-Replica Peptides
Two-Electrode Voltage Clamp
Analysis of Global and Specific Changes in the Disulfide Proteome Using Redox 2D-PAGE
Microautophagy in the Yeast Saccharomyces cerevisiae
Gel Electrophoresis Assays for Analyzing DNA Double-Strand Breaks in Saccharomyces cerevisiae at Var
Protocols for Use of Homologous Recombination Gene Targeting to Produce MicroRNA Mutants in Drosophi
Genetic Modification of Murine Hematopoietic Stem Cells by Retroviruses
Quantitation of Cocaine, Benzoylecgonine, Ecgonine Methyl Ester, and Cocaethylene in Urine and Blood
Assaying Cell-Cell Adhesion
多能干细胞（pluripotent stem cell）
核纤层蛋白(nuclear lamina protein)
Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry
Cell death detection in Xenopus embryos by ELISA
Examination of the Axial Skeleton of Fetal Rodents
Tissue Culture Methods
MAP Kinase Regulation of the Mitotic Spindle Checkpoint
Derivation and Manipulation of Murine Embryonic Stem Cells
Isolation and Characterization of Hepatic Stem Cells, or Oval Cells, from Rat Livers
Biochemical Analysis of Arginine Methylation in Transcription
胸腹水细胞染色体制备方法
Enzyme Immobilization on Nylon
Using Quantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Even
Competitive and Differential RT-PCR (CD-RT-PCR) for Measurement of Normalized Gene Expression Using
Overexpression or Downregulation of mTOR in Mammalian Cells
Control of the Interferon Response in RNAi Experiments
Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi
Screening for Protein-Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells
Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity
A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae
Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. cri
协助扩散
Methods to Study the Ras2 Protein Activation State and the Subcellular Localization of Ras-GTP in Sa
Joining RNA Molecules with T4 DNA Ligase
Semipermeabilized Cells to Study Procollagen Assembly
Approaches and Species in the History of Vertebrate Embryology
Novel Strategies for the Identification of Clock Genes Neurospora With Insertional Mutagenesis
着丝粒(centromere)
不能不知的细胞培养的全攻略
Rapamycin-Induced Enhancement of Vaccine Efficacy in Mice
Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells
Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion
Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila
Analysis of Common Mitochondrial DNA Mutations by Allele-Specific Oligonucleotide and Southern Blot
Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells
应力纤维(stree fibers)
Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line
Conventional Micropipette-Based Patch Clamp Techniques
Scale-Up of Suspension and Anchorage-Dependent Animal Cells
On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis
Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens
Measurement of Geminin Activity in Xenopus Egg Extracts
Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics: Constructing GFP-Tagged Motor Enz
实验室最常用的移液枪你用对了吗？
western-blot实验中ECL发光检测操作步骤
如何做好western-blot实验
蛋白质凝胶电泳注意事项
碱变性法提取质粒DNA
DNA 凝胶电泳注意事项
抗体标记的步骤
去除去污剂的可行性
PCR-SSCP实验步骤
无菌操作的注意事项及常见问题
cDNA文库构建详细步骤
SDS-PAGE的主要应用有哪些？
western-blot实验过程中常见问题及解答
重叠延伸PCR实验
普通PCR的基本原理和注意事项
western-blot化学发光为什么会出现高背景？
异丙醇法沉淀DNA
聚丙烯酰胺凝胶的配制
大肠杆菌转化实验（热击法）
如何对包涵体蛋白进行表达与复性
逆转录-聚合酶链反应(RT-PCR)
启动子甲基化调控的miR-148a-3p通过靶向MAP3K9抑制肺腺癌的进展
western-blot实验时，胶片上条带很弱怎么办？
实时荧光定量PCR实验步骤
基因克隆连接到T载体是不是多此一举？
western-blot实验步骤
染色质免疫共沉淀（ChIP）实验原理
蛋白质分离实验——细胞裂解方法
western-blot化学发光（ECL）出现高背景是什么原因？
实时荧光定量PCR技术简介
常用抗生素溶液有哪些？
western-blot实验常见问题及解决方案（enlight）
导致western Blot发光（ECL）“背景高”的原因有哪些？
蛋白质的长期保存——以冻干固体的形式保存
离心的种类和原理
如何提高qPCR反应的灵敏度与特异性？
磷酸钙转染法实验原理及步骤
琼脂糖凝胶电泳拖尾了怎么办？
寡聚（dT）-纤维素柱层析法分离纯化mRNA
SDS 碱裂解法制备质粒DNA: 少量制备
色谱法分离蛋白质
蛋白质印迹实验过程注意事项
为什么荧光原位杂交备受关注？
电穿孔和电融合技术原理及流程
在果蝇S2细胞中通过反义寡核苷酸抑制miRNA 功能
Western Blot化学发光(ECL)淬灭的原因？
PCR 标记DNA
western-blot化学发光时，出现非特异条带，该怎么办？
PCR实验最常见的9个问题及解决方案
限制酶和DNA 甲基化酶
SDS 碱裂解法制备质粒DNA: 大量制备
RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium
合子基因（zygotic gene）
Phylogenetic Inference and Parsimony Analysis
Knock-Down of Gene Expression in Hematopoietic Cells
Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protei
Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products
A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
Long-Term Cryopreservation of Thylakoid Membranes
Retroviral Transduction of FACS-Purified Hematopoietic Stem Cells
The Teratoma Assay: An In Vivo Assessment of Pluripotency
张力丝（tonofilaments）
Simplified PCR Assay for Detecting Early Stages of Multipotent Mesenchymal Stromal Cell Differentiat
Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Dire
Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay
Isolation of Stem and Precursor Cells from Fetal Tissue
NB2-cell proliferation assay
Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat Lungs
Unbiased Characterization of the Senescence-Associated Secretome Using SILAC-Based Quantitative Prot
Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat Lungs
Unbiased Characterization of the Senescence-Associated Secretome Using SILAC-Based Quantitative Prot
Isolation, Purification, and Cultivation of Murine and Human Keratinocytes
Micro RNA Profiling: An Easy and Rapid Method to Screen and Characterize Stem Cell Populations
Analysis of Telomere Dynamics in Mouse Spermatogenesis
Combining Lipidomics and Proteomics of Human Cerebrospinal Fluids
Assays of Osteogenic Differentiation by Cultured Human Mesenchymal Stem Cells
In Situ Hybridization to Sections of Xenopus Embryos
Kinetochore Microtubules, Analyzed by Correlated Light and Immunoelectron Microscopy
Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry
RLGS protocol
Principles of Membrane Electroporation and Transport of Macromolecules
In Vivo Detection of Oxidized Proteins: A Practical Approach to Tissue-Derived Mitochondria
Protein-Protein Interactions
复合物Ⅲ(complex Ⅲ)
Hydroxymethylated DNA Immunoprecipitation (hmeDIP)
Immortalization of Human Mesenchymal Stromal Cells with Telomerase and Red Fluorescence Protein Expr
Interaction of ES Cell Derived Neural Progenitor Cells with Natural Killer Cells and Cytotoxic T Cel
Lipidomic Analysis of Prostanoids by Liquid ChromatographyElectrospray Tandem Mass Spectrometry
Lipidomic Analysis of Biological Samples by Liquid Chromatography Coupled to Mass Spectrometry
肌节(sarcomere)
Gene-Targeting Strategies
神经递质(neurotransmitters)
In Vitro Hematopoietic Differentiation of Murine Embryonic Stem Cells
Study of Gap Junctions in Human Embryonic Stem Cells
Creation of Human Skin Equivalents for the In vitro Study of Angiogenesis in Wound Healing
Isolation and Culture of Porcine Embryonic Stem Cells
Near-Infrared Optical Proteolytic Beacons for In Vivo Imaging of Matrix Metalloproteinase Activity
Application of Proteomics to Posttransplantational Follow-Up
Preparation and Analysis of Plant and Plastid Proteomes by 2DE.
Comprehensive Transcriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents, and
Lentiviral Vector Gene Transfer into Human T Cells
Analysis of Early Epidermal Development in Zebrafish
Identification, Isolation, Characterization, and Banking of Human Dental Pulp Stem Cells
XC Assay of MoMLV Virus Stocks
Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo
Autogene Selections
RNA Affinity Tags for the Rapid Purification and Investigation of RNAs and RNAProtein Complexes
内含子（intron）
Cryopreservation and Freeze-Drying of Fungi
Preparation and Crystallization of RiboswitchLigand Complexes
成虫盘(imaginal discs)
Analyzing Myc in Cell Transformation and Evolution
Conventional and Immunoelectron Microscopy of Mitochondria
大鼠血管加压素（AVP）酶联免疫分析
Genetic Analysis of Meiotic Recombination in Schizosaccharomyces pombe
Cell-Cell Interaction in the Transcellular Biosynthesis of Novel -3-Derived Lipid Mediators
Pseudo-Immunolabelling With the AvidinBiotinPeroxidase Complex due to the Presence of Endogenous Bio
Studies on Carbohydrate Xenoantigens
Amplification of Fungal Genomes Using Multiple Displacement Amplification
Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Blood Cells in Coculture
Ultraviolet-Induced Apoptosis in Embryonic Stem Cells In Vitro
Automation of Fluorescent Differential Display With Digital Readout
Determination of Nuclear Localization Signal Sequences for Krppel-Like Factor 8
FISH and Immunofluorescence Staining in Chlamydomonas
Preparation of low density, collagenase-digested splenocytes Protocol
Isolation, Characterization, and Culture of Epithelial Stem Cells
Tissue-Specific KO of ECM Proteins
In Vitro Replication Assay with Mammalian Cell Extracts
常用的蛋白水解酶抑制剂
RNA 凝胶电泳注意事项
分子实验什么时候需要无菌操作？
siRNA表达载体的构建
Markers of Adult Neural Stem Cells
Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry
Neutrophil Motility In Vivo Using Zebrafish
OnLine Ozonolysis Methods for the Determination of Double Bond Position in Unsaturated Lipids
Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells
Expression Analysis of Murine Genes Using In Situ Hybridization With Radioactive and Nonradioactivel
细胞融合技术
Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells
Generation of Human Cell Lines Using Lentiviral-Mediated Genetic Engineering
Toxicity Methods for CPPs
The Latest Improvements in the Mouse Sperm Preservation
Culture of Isolated Human Adipocytes and Isolated Adipose Tissue
Integrin-Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs In Vitro and In Vivo
Detection of the Nuclear Poly(ADP-ribose)-Metabolizing Enzymes and Activities in Response to DNA Dam
Production of Membrane Proteins in Escherichia coli and Lactococcus lactis
Automated Video Image Analysis of Larval Zebrafish Locomotor Rhythms
Raman Microscopy of Bone
Detection of Terminal Transferase in Leukemia
In Vivo Assay for Tumor Cell Invasion
Detection of SPO11-Oligonucleotide Complexes from Mouse Testes
Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic An
MT Spindowns from Extracts
Retroviral Gene Transfer into Primary Human Natural Killer Cells
Isolation and Analysis of Lipid Rafts in CellCell Interactions
Differentiation of Embryonic Stem Cells to Retinal Cells In Vitro
Blood Cell Capture on Antibody Microarrays and Monitoring of the Cell Capture Using Surface Plasmon
Coimmobilization of Enzymes and Cells
The Generation of Embryoid Bodies from Feeder-Based or Feeder-Free Human Pluripotent Stem Cell Cultu
Targeted Gene Delivery by Virosomes*
Classical Genetic Manipulation
Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets
in situ Hybridization Protocols for Detection of Viral DNA Using Radioactive and Nonradioactive DNA
Assays for the Calcium Sensitivity of Desmosomes
PCR扩增产物出现杂带怎么办？
Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy
Whole mount TUNEL analysis of Xenopus embryos
Western blot实验最常见的10个问题及解决方案
Class II MHC Antigen Processing in Phagosomes
分子伴侣(molecular chaperones)
慢病毒感染细胞后，细胞长满48小时是否可以先传代，再等到72小时再喷霉素筛选吗
Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells: Genetic Selection and Potentia
Synchronization of Yeast
Qualitatively Monitoring Binding and Expression of the Transcription Factor Sp1 as a Useful Tool to
小鼠白细胞介素22（IL-22）酶联免疫分析
Western blot 中Tween-20的作用是什么？应该怎么用？
Cell Lineage Analysis in Xenopus Embryos
Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration
蛋白质的保存方法如何选择？
胞质膜(cytoplasmic membrane)
ChIP超详细实验步骤
Methods to Study Phosphorylation and Activation of the Hormone-Sensitive Adipocyte Phosphodiesterase
Sequence Analysis of the Whole Mitochondrial Genome and Nuclear Genes Causing Mitochondrial Disorder
粘着连接(adherens junctions, zonula adherens)
Purification of Nuclei and Preparation of Nuclear Envelopes from Skeletal Muscle
Quantitative Determination of Collagen Crosslinks
Telomerase Inhibition and Telomere Targeting in Hematopoietic Cancer Cell Lines with Small Non-Nucle
ELISA不得不懂的实验原理
mRNA的分离纯化原理
蛋白质浓度的测定
UHRF1如何调控AMPK的活性？在细胞代谢中又有哪些关键作用？
WB发光检测中常见的问题及解决方法
型核酸分子荧光探针——分子信标的原理及应用
慢病毒感染后72小时，效率低，细胞已经长满，有什么办法补救？
应用长链dsRNA 抑制基因表达
测序胶的灌制、电泳及处理
亲和层析纯化抗体,请问纯化后收集到的抗体溶液，加甘油或者醇类，糖类的话，浓度一般是加多少
建立透析的方法步骤
微型凝胶电泳后使用溴化乙锭（EB）估算条带中DNA 数量
抗体的使用以及储存方式
缺口翻译标记DNA
RNAi、miRNA及siRNA的区别和联系
如何制备双链siRNA？
影响western-blot实验的因素有哪些？
siRNA表达载体的构建成功要点
真空浓缩仪的使用方法
siRNA是从牵牛花过表达发现的吗
离心机的种类
利用有机溶剂分离纯化高分子质量DNA
DNA 样品的酚抽提
亲和层析纯化抗体，如何减少洗脱后中和沉淀的出现？
封闭问题可大可小，千万不能掉以轻心
常用电泳缓冲液的配制方法
western-blot实验注意事项
PCR （多聚酶链式反应）
用测序酶进行标记／终止测序反应常见问题
早期游泳对Shank3基因敲除的自闭症大鼠模型的行为和纹状体转录组的影响
DNA 的 PCR 扩增
细胞转染siRNA后，目标基因的表达反而升高的原因是什么？
佛手柑素（BM）或可被开发成预防骨质疏松症的食品成分或补充剂
聚丙烯酰胺凝胶电泳注意事项
将DNA 导入细胞和微生物
建立透析的方法步骤
七大热点技术：靶向基因治疗、空间多组学……
蛋白质结构预测
siRNA表达载体的构建成功要点
双向电泳相关试剂配制
怎样做好荧光原位杂交实验（FISH）
细胞伤口愈合实验
硝酸还原酶活力测定（活体法）-磺胺比色法
叶绿体被膜完整性的测定
植物细胞的活体染色与死活鉴定
分光光度法测定叶绿素含量
还原糖含量测定-用斐林试剂热滴定法
还原糖含量测定-砷钼酸比色法
植物体内可溶性糖含量的测定(蒽酮法)
植物原生质体的制备
显微摄影术
细胞凝集反应与细胞膜的渗透性
叶绿体的分离与荧光观察-等渗法
植物细胞结构与植物徒手切片观察试验
植物细胞分裂和植物分生组织实验
茎的形态与初生结构
种子与幼苗的观察
茎的次生结构实验-观察切片
酶的激活和抑制实验
还原糖和总糖的测定实验
淀粉酶活性的测定实验
聚丙烯酰胺凝胶电泳法测定大麦小麦种子纯度实验
淀粉酶的诱导、提取和活性测定实验
植物组织水势的测定实验
植物伤流量的测定实验
根系活力的测定实验
蒸腾强度的容量法测定实验
蒸腾强度的称重伐测定实验
叶绿体色素的提取、分离、性质和定量测定实验
光合强度的测定实验
呼吸强度的测定实验
植物抗逆性的鉴定实验
植物缺水程度的测定实验
过氧化氢酶的活性测定实验
小麦幼根中液泡的形态学观察实验
液泡的分离实验
原生质等电点测定实验
原生质运动的观察实验
植物组织的渗透势测定实验
叶绿素的荧光现象实验
叶绿素皂化实验
叶绿素与类胡萝卜素的吸收光谱实验
氢和铜对叶绿素中镁的代替作用实验
叶绿体色素的分离纸层析法实验
叶绿素的定量测定实验
植物光合强度测定实验
植物呼吸强度测定实验
离体线粒体的氧化作用和磷酸化作用实验
植物去氢酶的简易鉴定实验
植物过氧化氢酶的简易鉴定实验
植物组织小液流法水势测定实验
植物伤流液的成分分析实验
植物根系对矿质元素的选择吸收实验
单盐毒害与混合盐的拮抗作用实验
植物的溶液培养与矿质元素缺乏症实验
种子生活力的快速鉴定实验
红墨水染色法测定种子活性实验
荧光法检测种子实验
植物生长区域分格法测量实验
双子叶植物茎的生长区域分格法测量实验
双子叶植物叶片生长区域测定试验
单子叶植物茎、叶的生长区域测定实验
生长素类物质对根芽生长影响实验
芽鞘伸长法测定生长素类物质的浓度和效价实验
绿豆根形成法测定生长素类物质的浓度或效价实验
赤霉素类物质浓度测定实验
细胞分裂素的保绿与阻止衰老的作用实验
细胞分裂素对菜豆叶片生长和衰老的效应实验
用棉花幼苗外植体法测定脱落酸的活性实验
用萝卜子叶增重法测定细胞分裂素类物质的浓度或效价实验
黄化豌豆幼苗的“三重反应”实验
乙烯的催熟作用实验
乙烯诱导雌花形成实验
生长素和乙烯对叶片脱落的效应实验
开花刺激物在短日植物中通过嫁接的传递实验-第三阶段：嫁接植株的管理及观察记载
开花刺激物在短日植物中通过嫁接的传递实验-第二阶段供体与受体的嫁接
开花刺激物在短日植物中通过嫁接的传递实验-第一阶段：嫁接植株的准备
花粉管的生长及其向化性实验
植物体内游离脯氨酸的测定实验
根际pH的显色测定实验
希尔反应的观察实验
植物离体叶片失水表型观察及其失水率测定实验
植物组织中自由水含量的测定实验
植物组织总含水量的测定实验
环境因子对植物吐水的影响实验
植物蒸腾速率测定实验
小孔扩散观察实验
植物气孔渗入法检测实验
印迹法植物气孔检测实验
固定法植物气孔检测实验
α－萘胺法植物根系活力测定实验
根系体积测定实验
植物根系伤流液中氨基酸总量的测定实验
植物伤流液中氨基酸成分分析实验
植物的溶液培养和缺乏必需元素时的症状实验
单盐毒害作用及离子间的对抗作用实验
离体法植物体内硝酸还原酶活性的测定实验
活体法检测物体内硝酸还原酶活性实验
植物体内硝态氮含量的测定实验
叶绿素的理化性质测定实验
水稻种子萌发对氧的需要实验
Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb
An Overview of HLA Typing for Hematopoietic Stem Cell Transplantation
Identification of Mutations in the Kir6.2 Subunit of the KATP Channel
Detection by TUNEL labeling
Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells
Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells
In Situ Hybridization for Metalloproteinases and Their Inhibitors
Profiling the Metabolic Signature of Senescence
COPⅡ被膜小泡(COPⅡ coated vesicle)
骨髓间充质干细胞向肝细胞诱导
Isolation and Purification of Vertebrate DNAs
Magnetic Resonance Imaging Analysis of Embryos
原代培养(primary culture)
Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays
Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells
In Vitro Fabrication of Engineered Human Skin
Using Somatic-Cell Nuclear Transfer to Study Aging
Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal
Immunosubtraction as a Means of Typing Monoclonal and Other Proteins in Serum and Urine
Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for
Solid Tumor DNA Ploidy Analysis
Pheromone Halo Assay
Assaying Phosphoinositide Phosphatases
Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells
细胞培养――细胞生长和细胞毒性
Transport of Antisense Across the Blood-Brain Barrier
Ier1 p: A Kinase and Site-Specific Endoribonuclease
Measuring CellCell Tugging Forces Using Bowtie-Patterned mPADs (Microarray Post Detectors)
Predictive Models for Cellular Signaling Networks
Dual-Incision Assays for Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site
In Vitro Mutagenesis to Define Functional Domains
A Method for the Generation of Conditional Gene-Targeted Mice
Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial
Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis
Electrically Mediated Reporter Gene Transfer into Normal Rat Liver Tissue
Murine Hematopoietic Stem Cell Transduction Using Retroviral Vectors
Yeast Pre-mRNA Splicing Extracts
In Situ Hybridization to Sections (Nonradioactive)
Experimental Cell Transplantation Therapy in Rat Myocardial Infarction Model Including Nude Rat Prep
Cultivation, Spore Production, and Mating
Fluorescence Microscopy Study of Rap1 Subcellular Localization
自溶作用(autolysis)
Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicro
Xenopus laevis Oocytes
Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions
Culture of Choroid Plexus Epithelial Cells and In Vitro Model of BloodCSF Barrier
Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patte
Comprehensive Identification of miRNA Target Sites in Live Animals
How The Mother Can Help: Studying Maternal Wnt Signaling by Anti-sense-mediated Depletion of Materna
细胞遗传学――染色体
Pre-embedding Immunogold Localization of Antigens in Mammalian Brain Slices
Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell D
Immunohistochemical Approaches for the Study of Spermatogenesis
$Peptidome Analysis of Mouse Liver Tissue by Size Exclusion Chromatography Prefractionation$
Peptidome Analysis of Mouse Liver Tissue by Size Exclusion Chromatography Prefractionation
Functional Analysis of Cytoskeletal Components in the Developing Visual System of Drosophila
Determining Protein Half-Lives
Screening for Positive Clones Generated by Differential Display
Preparation of recombinant fibronectin fragments for functional and structural studies
细胞培养板的选择
Light, Photoreceptors, and Circadian Clocks
Assaying Chromosome Pairing by FISH Analysis of Spread Saccharomyces cerevisiae Nuclei
Solid Phase Assays for Studying ECM ProteinProtein Interactions
Efficient Transfer of HSV-1 Amplicon Vectors Into Embryonic Stem Cells and Their Derivatives
Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids in Urine, Serum, and Plasma U
Covalent Immobilization of Enzymes Using Commercially Available CDI-Activated Agarose
Three-Dimensional Culture of Human Breast Epithelial Cells: The How and the Why
Resistance Loading and Signaling Assays for Oxidative Stress in Rodent Skeletal Muscle
Establishment of Mouse Epithelial Lung Cell Strains and Cell Lines
Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress
An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs
Study of G-Protein-Coupled Receptor-Protein Interactions by Bioluminescence Resonance Energy Transfe
Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin
大鼠铁蛋白(Ferritin)酶联免疫分析
Expression of Foreign Genes in Cultured Insect Cells Using a Recombinant Baculovirus Vector
Detection of Activated STAT Proteins
Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries
Preparation and Direct Automated Cycle Sequencing of PCR Products
Methyl-Combing: Single-Molecule Analysis of DNA Methylation on Stretched DNA Fibers
Visual Markers for Detecting Gene Conversion Directly in the Gametes of Arabidopsis thaliana
Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen
MMP-Mediated Disruption of Claudin-5 in the BloodBrain Barrier of Rat Brain After Cerebral Ischemia
Proside: A Phage-Based Method for Selecting Thermostable Proteins
The Biology of Cyclins and Cyclin-Dependent Protein Kinases: An Introduction
Isolation of Mouse Thymic Dendritic Cell Precursors
Cellular Studies for In Vitro Modeling of Xenogeneic Immune Responses
Oxidative Phosphorylation: Synthesis of Mitochondrially Encoded Proteins and Assembly of Individual
Isolation and Functional Studies of Human Fetal Gastric Epithelium in Primary Culture
Centriole Duplication, Centrosome Maturation and Spindle Assembly in Lysates of Spisula solidissima
位置信息(positional information)
Large-Scale Culture and Preparation of Sea Urchin Embryos for Isolation of Transcriptional Regulator
Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate
PCR-Mediated Chemical Mutagenesis
Proteomic Analysis of Human Mesenchymal Stem Cells
Membrane Permeabilization with Bacterial Toxins
Introduction of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases
Method: Maintaining Lymphoblastoid Cell Lines
In Vivo Transplantation and Tooth Repair
Matrix MetaIIoproteinase Substrate Binding Domains, Modules and Exosites: Overview and Experimental
Isolation and Differentiation of Medaka Embryonic Stem Cells
Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Pro
Single-Strand Conformational Polymorphism (SSCP) for the Identification of Endomycorrhizal Fungi
动粒(kinetochore)
Culture of Adipose Tissue and Isolated Adipocytes
Development of Multiple-Well Plate Biological Assays
Detection of Rhythmic Bioluminescence From Luciferase Reporters in Cyanobacteria
Keeping Two Animal Systems in One Lab A Frog Plus Fish Case Study
Expression and Purification of Membrane-Type MMPs
Chimeric Animals and GermlineTransmission
Creating 2DE Databases for the World Wide Web
Interspecific Chimeras in Avian Embryos
Environmental Scanning Electron Microscope Imaging of Vesicle Systems
Drosophila melanogaster as a Model System to Study Mitochondrial Biology
Methodology of Aniline Blue Staining of Chromatin and the Assessment of the Associated Nuclear and C
Mitochondrial Fusion In Vitro
Enzyme-Linked Immunosorbent Assay for Screening of Antibodies in Hybridoma Supernatants
The Use of JAK-Specific Inhibitors as Chemical Biology Tools
Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration
Ultrastructural Detection of Nucleic Acids on Thin Sections of Tissue Embedded in Hydrophilic Resin
Integrase Defective, Nonintegrating Lentiviral Vectors
Transwell Invasion Assays
Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila
Antisense Efficacy: Site-Restricted In Vivo and Ex Vivo Models
Methods for Human Embryonic Stem Cells Derived Cardiomyocytes Cultivation, Genetic Manipulation, and
In Vitro and In Vivo Methods for Assessing FcRn-Mediated Reverse Transcytosis Across the BloodBrain
Harvesting lymphocytes from Peripheral Blood
Embryological Manipulations in Zebrafish
Transdifferentiation of Mature Hepatocytes into Bile Duct/ductule Cells Within a Collagen Gel Matrix
Functional Analysis of Human Islets of Langerhans Maintained in Culture
Isolation and Purification of Insect DNA
Molecular Control of Mammalian Myoblast Fusion
Isolation and Characterization of Adult Neural Stem Cells
Detection and Identification of the Four Malaria Parasite Species Infecting Humans by PCR Amplificat
Generation, Identification, and Characterization of Repair-Defective Mutants of Arabidopsis
Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons
Epidermal Lineage
小鼠白细胞介素23（IL-23）酶联免疫分析
Measuring Equilibrium and Kinetic Constants Using Gel Retardation Assays
H-Thymidine Uptake by Cultured Cells
Use of Adipose-Derived Stem Cells in High-Throughput Screening to Identify Modulators of Lipogenesis
Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer
Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions
Phage-Displayed Antibodies to Detect Cell Markers
Grb2蛋白(growth factor receptor-bound protein 2)
核型(karyotype)
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
Receptor Mutagenesis Strategies for Examination of Structure-Function Relationships
Composite Electrospun Nanofibers for Influencing Stem Cell Fate
Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells
Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models
Affinity Precipitation of Proteins Using Metal Chelates
Analysing Caspase Activation and Caspase Activity in Apoptotic Cells
膜运输蛋白(membrane transport protein)
In Vitro Migration Assays of Neural Stem Cells
Collagen Gel Culture of the Human Hematopoietic Progenitors CFU-GM, CFU-E, and BFU-E
脂质体转染后的培养基选择
Reconstitution of Membrane Transporters
Retroviral Infection of T-Cell Precursors in Thymic Organ Culture
Examining the Endogenous Antioxidant Response Through Immunofluorescent Analysis of Nrf2 in Tissue
Craniofacial Skeletel Morphogenesis In Vitro
Analysis of Cell Migration Using Caenorhabditis elegans as a Model System
Bacterial Genome Sequencing
侧向扩散(lateral diffusion)
Methods for Culturing Human Embryonic Stem Cells in a Xeno-Free System
纤维切割蛋白(filament-severing protein)
Electrotaxis and Wound Healing: Experimental Methods to Study Electric Fields as a Directional Signa
Proteomic Analysis of Scaffold Proteins in the ERK Cascade
Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells
Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents: Heterologous Expression
Isolation and Characterization of Mesenchymal Stem Cells
猪白细胞介素-6（IL-6）酶联免疫分析（ELISA）
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media
Isolation, Culture, and Potentiality Assessment of Lung Alveolar Stem Cells
Using Antibodies to Analyze Polyglutamine Stretches
The Dorsal Skinfold Chamber: Studying Angiogenesis by Intravital Microscopy
Development of Physiological Models to Study Stress Protein Responses
Cytogenetic Analysis of Cell Lines
The Use of Electroporated Bovine Spermatozoa to Transfer Foreign DNA into Oocytes
Growth and Stratification of Epithelial Cells in Minimal Culture Conditions
Transfer of Eukaryote Suspension Cultures
Endothelial Colony-Forming Progenitor Cell Isolation and Expansion
细胞示踪荧光探针的基础知识概述
Therapeutic Applications of Encapsulated Cells
Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods)
Fluorimetric DNA Assay of Cell Number
Measurement of Environmental Particulate Uptake by Lung Cells Using Flow Cytometry
Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry (SIMS)
Dictyostelium Slug Phototaxis
Following the Fate of Neural Progenitors by Homotopic/Homochronic Grafts in Xenopus Embryos
The Use of Selective Enrichment for the Isolation of Species-Specific DNA Probes for Insects
Protein Kinase-A Directed Antisense Therapy of Tumor Growth In Vivo: The Antisense Effects Outlast A
Immobilization of Enzymes on Monofunctional and Heterofunctional Epoxy-Activated Supports
Isolation of Human and Mouse Hematopoietic Stem Cells
Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System
Cloning of Hybridomas
植物体内抗坏血酸氧化酶活性的测定实验
植物体内多酚氧化酶活性的测定实验
植物蔗糖含量的测定实验
植物体内可溶性蛋白和非可溶性蛋白含量的测定实验
生长素对绿豆苗发根的影响实验
细胞分裂素对离体子叶的保绿效应实验
植物生长延缓剂多效唑的壮苗效应实验
种子生活力的剥皮法快速测定实验
酸性靛兰染色检测种子活力实验
荧光法检测种子实验
光对植物生长的影响实验
植物光周期现象的观察实验
植物体内丙二醛含量的测定实验
植物体内过氧化物酶活性的测定实验
植物细胞质膜透性的测定实验
植物体内氧自由基含量的测定实验
植物花粉母细胞减数分裂的染色体观察实验
植物花粉母细胞减数分裂制片实验
植物根尖压片实验
染色体组型分析实验
连锁基因的遗传分析实验
瓦氏呼吸机植物组织呼吸速率的测定试验
气流法检测植物呼吸速率实验
基因的分离独立分配和互作实验
辐射对植物染色体的诱变作用实验
植物DNA的提取与定量分析实验
染色体数目变异实验
染色体结构变异实验
诱变物质的微核测定实验
水在植物体内的运输途径与速度及维管束运输潜力实验
转基因植株的选择实验-转基因植物的分析
转基因植株的选择实验-转基因植株的生长和选择
基因枪法转化小麦实验-轰击后未成熟盾片培养
基因枪法转化小麦实验-使用 PDS-1000/He 基因枪轰击
基因枪法转化小麦实验-DNA包裹金粉颗粒
基因枪法转化小麦实验-准备供体材料
农杆菌介导的小麦新鲜离体幼胚的转化实验-转化效率的计算
农杆菌介导的小麦新鲜离体幼胚的转化实验-转基因植株的鉴定及种子的收获
农杆菌介导的小麦新鲜离体幼胚的转化实验-选择
农杆菌介导的小麦新鲜离体幼胚的转化实验-胚性愈伤的诱导和再生
农杆菌介导的小麦新鲜离体幼胚的转化实验-幼胚的接种
农杆菌介导的小麦新鲜离体幼胚的转化实验-根癌农杆菌接种外植体的准备（见注 6)
农杆菌介导的小麦新鲜离体幼胚的转化实验-根癌农杆菌菌液的准备
小麦花器官转化实验
植物原位接种介导的小麦高效农杆菌转化方法实验
基因枪法转化大麦实验
农杆菌介导的大麦转化方法实验
燕麦转基因及其在提高渗透胁迫耐受中的应用
基因插入位点和模式
荧光原位杂交技术检测植物基因组中整合的转基因片段
实质等同性（转录组学）
实质等同性（蛋白质组学）
实质等同性（代谢组学）
转基因作物田间试验的设计与管理
三氯乙酸-丙酮法提取植物全蛋白
顽拗性植物组织的蛋白质组学研究（苯酚法提取蛋白质）
谷类植物种子蛋白质提取
木质部和韧皮部汁液蛋白质提取
木本植物蛋白质提取实验
拟南芥叶绿体蛋白质组学分析
植物线粒体制备及其亚结构分级分离
根分生细胞核蛋白质提取
核蛋白提取实验
紫花苜蓿茎细胞壁蛋白质提取
植物质膜蛋白的提取和溶解
双向电泳前去垢剂和离液剂对蛋白质的增溶作用
植物蛋白质组学中的双向电泳技术
双向凝胶荧光染色与成像
二维凝胶的定量分析
蛋白质组数据的多元分析
2D 凝胶的蛋白 Edman 测序
肽质量指纹图谱—MALDI-TOF 法鉴定蛋白质
利用纳升级液相色谱- 串联质谱进行蛋白质鉴定
水稻叶片和根蛋白质的纳升级二维液相色谱串联质谱
膜蛋白的分离、鉴定及其功能分析—GFC/IEC/SDS-PAGE 和 MALDI-TOF-MS 方法
2-DE 蛋白质组学的 PROTICdb 数据库
western blot 检测蛋白激酶磷酸化水平
蛋白质磷酸化
磷酸化蛋白鉴定实验
植物蛋白质组学和糖基化
鉴定分析植物蛋白复合物的蓝绿非变性凝胶电泳
完整蛋白质从 SDS-PAGE 胶中的电洗脱及其 MALDI-TOF MS 分析
植物蛋白芯片的构建和抗原抗体相互作用的研究
利用植物蛋白质芯片研究蛋白磷酸化实验
超薄切片技术
显微化学方法
压片法
组织离析法
整体封藏法
石蜡切片法
徒手切片法
临时装片法
植物标本的采集和制作实验-孢子植物标本采集与制作
植物标本的采集和制作实验-其他标本的制作
植物标本的采集和制作实验-腊叶标本的采集与制作
种子植物鉴定实验
植物细胞中淀粉粒、糊粉粒和油滴的观察和检验实验-三种后含物的综合鉴定
植物细胞中淀粉粒、糊粉粒和油滴的观察和检验实验-脂肪鉴定
植物细胞中淀粉粒、糊粉粒和油滴的观察和检验实验-糊粉粒（蛋白质）鉴定
植物细胞中淀粉粒、糊粉粒和油滴的观察和检验实验-淀粉粒检验
花青素和无机盐结晶体的观察
硅藻门鉴定
黄藻门鉴定
裸藻门鉴定
蓝藻门鉴定
褐藻门鉴定
红藻门的鉴定
轮藻门的鉴定
绿藻门鉴定
真菌门鉴定
黏菌门鉴定
真蕨亚门鉴定
鉴定桉叶亚门
蕨类植物鉴定
金缕梅亚纲鉴定
木兰亚纲鉴定
被子植物(Ⅳ)菊亚纲-菊科鉴定
被子植物(Ⅳ)菊亚纲-忍冬科鉴定
被子植物(Ⅳ)菊亚纲-玄参科鉴定
被子植物(Ⅳ)菊亚纲-木犀科鉴定
被子植物(Ⅳ)菊亚纲-唇形科鉴定
被子植物(Ⅳ)菊亚纲-旋花科鉴定
被子植物(Ⅳ)菊亚纲-茄科鉴定
植物异常结构的观察
植物整体形态结构观察
植物形态多样性的观察实验
藻类植物分离培养
藻类植物采集方法
植物组织和细胞的显微化学染色实验-植物组织中酶的检测方法
植物组织和细胞的显微化学染色实验-检测植物细胞中核酸的方法
植物组织和细胞的显微化学染色实验-显示植物细胞后含物的方法
植物组织和细胞的显微化学染色实验-显示细胞壁化学组成的方法
开花与传粉过程的观测实验
花粉萌发与花粉管生长的观察实验
浮游植物的调查和鉴定实验
植物候期的观察与记录实验
植物花粉形态观察研究实验
植物种群的种群数量与年龄结构调查实验-基本方案
花青素表达的定量
荧光蛋白（FP) 的显微检测
利用冷型 CCD 像机进行 LUC 的活体检测
植物提取物中LUC活性的检测
GUS 活性的组织化学检测
MUG 荧光分析
报告基因实验-利用MUG检测 β-葡萄糖醛酸酶（GUS）
不良环境对植物的伤害实验
番茄叶和根二维差异凝胶电泳（DIGE)
RNA介导的基因沉默
TPS－1植物光合系统测定实验
赤霉素对α－淀粉酶诱导形成实验
TTC法根系活力的测定实验
脂肪浸提——索氏脂肪浸提法实验
植物 DNA 提取实验-一种简单实用的提取植物DNA实验
维生素C含量的测定实验
氯化三苯基四氮唑（TTC）法测定种子实验
裸子植物的鉴定实验-柏科鉴定
裸子植物的鉴定实验-松科鉴定
裸子植物的鉴定实验-银杏科鉴定
裸子植物的鉴定实验-苏铁科鉴定
植物细胞渗透势的测定
苯丙氨酸解氨酶（PAL）
植物气孔开闭状况的测定
钾离子对气孔开度的影响实验
植物蒸腾速率的测定
植物组织中酷类物质含量的测定
植物的溶液培养和缺素症观察
硝酸还原酶活性的测定实验
硝酸还原酶活性的测定
抗坏血酸含量的测定
单盐毒害及离子间拮抗现象
生长素类物质对根芽生长的影响
植物激素对愈伤组织形成和分化的影响
原子吸收法测定植物矿质元素
酶联免疫吸附法测定植物激素含量
高效液相色谱法测定植物的内源激素含量
气相色谱法测定乙烯含量
赤霉素对α-淀粉诱导合成的影响
植物花粉管生长的测定
植物叶绿体的分离制备
植物春化作用和光周期现象的观察
植物组织逆境伤害程度的测定
植物组织种丙二醛含量的测定
植物组织中ATP酶活力的测定
超氧化物歧化酶（SOD）活力测定
希尔反应的观察与测定
过氧化氢酶（POD）活性的测定
过氧化氢酶（CAT ）活性的测定
多酚氧化酶（PPO）活性的测定
叶绿体色素的提取、分离和理化性质观察
叶绿体色素的定量测定-混合液浸提法
核酮糖-1，5-二磷酸羧化酶/加氧酶活性的测定
磷酸烯醇式丙酮酸羧化酶活性的测定
改良半叶法测定叶片光合速率
LI-6400便携式光合仪测定植物叶片的气体交换
植物叶绿素荧光参数的测定
细胞分裂素对萝卜子叶的保绿作用
植物呼吸速率的测定
抗坏血酸过氧化物酶 (AsA-POD)活性的测定
苯丙胺酸解氨酶（PAL）活性的测定
植物体内游离脯氨酸含量的测定
《核酸提取技术》相关实验技术----q-PCR
《核酸提取技术》相关实验技术----DNA提取
植物体内甜菜碱含量的测定
过氧化氢含量的测定
氧自由基产生速率的测定
植物组织中蔗糖酶活力的测定
纤维素酶活力的测定
植物组织中游离氨基酸总量的测定
植物组织中可溶性糖含量的测定
谷物中淀粉含量的测定
果实、蔬菜中有机酸含量的测定
高粱籽粒中单宁含量的测定
植物组织中酚类物质含量的测定
植物生物碱含量的测定
植物黄酮化合物含量的测定
徒手切片实验
植物根茎叶结构观察-叶的形态和结构观察
植物根茎叶结构观察-茎的次生结构观察
植物根茎叶结构观察-茎的形态和初生结构观察
植物根茎叶结构观察-双子叶植物根的次生结构观察
植物根茎叶结构观察-根的初生结构观察
植物根茎叶结构观察-根尖的内部构造观察
植物根茎叶结构观察-根尖的外形与分区观察
植物激素类物质的生物鉴定-赤霉素的生物鉴定
植物激素类物质的生物鉴定-细胞分裂素的生物鉴定
植物激素类物质的生物鉴定-生长素的生物鉴定
种子生活力的快速测定-红墨水染色法测定种子生活力
种子生活力的快速测定-氯化三苯基四氮嗖（TTC）法测定种子生活力
植物根系活力的测定-TTC法测定根系活力
植物根系活力的测定-α-萘胺氧化法测定植物根系活力
花粉活力的测定-氯化三苯基四氮唑（TTC）法测定花粉活力
花粉活力的测定-碘-碘化钾染色测定花粉活力
花粉活力的测定-花粉萌发测定法测花粉活力
光对需光种子萌发的影响实验-有红光和远红光光源对需光种子萌发的影响实验
光对需光种子萌发的影响实验-无红光和远红光光源对需光种子萌发的影响实验
整体透明制片实验-子房整体透明法
整体透明制片实验-乳酸酚透明法制作根尖整体透明封片
组织离析制片技术-根尖的离析压片法
组织离析制片技术-木材的离析制片法
植物石蜡切片技术
被子植物花图式与花程式
植物细胞的基本结构及后含物观察
植物细胞器的荧光标记及观察
植物染色体核型分析
植物细胞程序性死亡的TUNEL检测
植物细胞微丝骨架的活体观察
花粉活力与柱头可授性检测
花粉体外萌发及花粉管生长的观察
植物花粉管向胚珠的定向生长
人工诱导针叶树创伤树脂道的形成
植物分子标记及应用-扩增片段长度多态性标记
植物分子标记及应用-简单序列重复标记
植物分子标记及应用-随机扩增多态DNA标记
植物分子标记及应用-限制性片段长度多态性标记
植物分子标记及应用-同工酶标记
植物遗传多样性检测
植物组织水势的测定
植物分子系统进化树的构建
植物组织含水量的测定
转基因小鼠制备实验
大鼠胰腺移植模型制作
肿瘤动物模型建立实验-悬尾实验（TST）
肿瘤动物模型建立实验-自发型肿瘤动物模型
肿瘤动物模型建立实验-诱发性肿瘤动物模型
藻酸盐包裹实验
激光扫描共聚焦显微镜使用说明
实验动物注射给药法-水中毒实验（water intoxication）
实验动物注射给药法-皮下注射、肌肉注射、静脉注射、动脉注射
实验动物的取血实验
实验动物的麻醉和处死
荧光显微镜观察肿瘤细胞凋亡
人肝癌裸鼠皮下-肝原位移植瘤模型的建立实验-间接原位移植模型
裸鼠前列腺原位肿瘤模型的建立实验
人胰腺癌裸小鼠模型的建立实验
人胃癌裸鼠原位种植转移模型的建立实验-细胞悬液原位种植法
人胃癌裸鼠原位种植转移模型的建立实验-胃囊法/缝挂法
人胃癌裸鼠原位种植转移模型的建立实验-优化胶粘贴法
人胃癌裸鼠原位种植转移模型的建立实验-OB胶粘贴法
人肝恶性淋巴瘤裸小鼠原位和皮下移植模型的建立实验
人胃癌高转移模型的建立实验-肿瘤组织块原位移植法
人胃癌完整组织块裸鼠原位种植高转移模型的建立实验-完整组织块原位种植法
人胃癌组织块裸鼠胃癌原位移植模型的建立实验-纤维蛋白原-凝血酶法
昆明小鼠原位胃癌移植模型的建立实验-皮下移植块原位移植法
人非小细胞肺癌裸鼠原位种植转移模型的建立实验
人肺癌裸鼠原位移植模型的建立实验-外科原位移植法
前列腺原位肿瘤模型的建立实验-原位种植法
人弥漫性大B细胞淋巴瘤小鼠肿瘤模型的建立实验-皮下种植法
动物体内抑瘤实验-生物发光标记法
药物对动物腹腔巨噬细胞的影响实验-流式细胞术法
药物对动物体内淋巴细胞增殖影响实验-细胞培养技术
药物对动物体内溶血素含量的影响实验-细胞培养技术
体外抗药肿瘤细胞模型的建立实验-细胞培养技术
体内耐药细胞株的建立实验-药物耐受实验
抗微管药物实验
成年小鼠的灌流实验
寄生虫标本的包装与邮寄实验
寄生虫标本的保存实验-浓集法
小鼠睾丸、附睾及输精管精子采集、运动能力检测与结构观察实验-血球计数法
小鼠超数排卵及附植前不同发育阶段胚胎采集与结构观察实验-显微镜观察法
尿钠的测定实验-比浊法
实验动物组织标本的制备实验-解剖法
鲤鱼的外形和内部解剖实验
鱼纲分类实验
哺乳纲分类实验
兔的外形及解剖实验
鸟纲分类实验
青蛙（或蟾蜍）的外形和解剖实验
家鸽(或家鸡)的外形及解剖实验
两栖纲及爬行纲分类实验
氯胺酮、硫喷妥钠对家兔呼吸和循环系统的影响实验-颈部皮下浸润麻醉及耳缘静脉注射
抗高血压药的降压作用实验-颈总动脉插管
肌松药对胫前肌的作用实验-电刺激
局部麻醉
局麻药毒性作用的比较实验-腹腔注射
普鲁卡因的椎管麻醉作用实验-椎管内注射
药物对局麻药中毒的保护作用-腹腔注射
泮库溴铵和琥珀胆碱对家兔作用的比较实验-肌肉注射
实验动物的外科技术及急救实验
实验动物的处死及尸体处理实验
实验动物的标记
小白鼠抓取和固定实验
大白鼠抓取实验
家兔的抓取与固定实验
狗的抓取与固定实验
蛙的抓取与固定实验
大小鼠的灌胃实验
兔和狗的灌胃实验
狗静脉注射实验
家兔静脉实验
大小鼠静脉注射实验
蛙类的静脉注射实验
蛙的淋巴囊注射实验
大鼠、小鼠针刺尾静脉取血实验
大鼠、小鼠剪尾尖取血实验
大鼠、小鼠眼部取血实验
大鼠、小鼠大血管取血实验
大鼠、小鼠心脏取血实验
大鼠、小鼠断头取血实验
豚鼠耳缘剪口采血实验
豚鼠心脏采血实验
豚鼠股动脉采血实验
豚鼠背中足静脉取血实验
家兔耳部取血实验
家兔大血管取血实验
家兔心脏取血实验
狗和猫耳缘静脉取血实验
狗和猫前、后肢静脉取血实验
狗和猫颈静脉取血实验
清醒大鼠的血压测定实验
麻醉大鼠动脉血压的测定实验（直接测定法）
蛙或蟾蜍坐骨神经－腓肠肌标本的制备实验
离体蛙心标本的制备实验
离体主动脉条的标本制备实验
豚鼠离体气管标本制备实验
麻醉家兔、大鼠中心静脉压的测定实验
麻醉家兔、大鼠左室内压与左室内压变化速率的测定实验
麻醉家兔、大鼠右室内压的测定实验
麻醉家兔、大鼠肺动脉压的测定实验
麻醉家兔、大鼠心电图的测定实验
刺激强度、频率对骨骼肌收缩的影响实验
尼可刹米对抗度冷丁抑制呼吸作用实验
药物对家兔动脉血压的作用实验
血浆胶体渗透压降低在水肿发生中的作用实验
家兔血液酸碱度变化与血气分析实验
体液改变在家兔急性失血中的代偿作用实验
蟾蜍梭传入冲动的测定实验
苯海拉明对组胺的竞争性拮抗作用实验
药物的镇痛作用实验
有机磷酸酯类中毒与解救实验
磺胺类药物的药代动力学参数测定实验
肺组织结构形态学观察实验
大叶性肺炎形态学观察实验
小叶性肺炎形态学观察实验
胃组织结构形态学观察实验
慢性胃溃疡形态学观察实验
胃癌形态学观察实验
淋巴结转移性腺癌形态学观察实验
肝脏形态学观察实验
急性普通型肝炎形态学观察实验
门脉性肝硬化形态学观察实验
肝细胞癌形态学观察实验
骨骼肌收缩的力学分析实验
负荷与骨骼肌收缩的关系实验
蟾蜍心活动起搏点的观察实验
观察蟾蜍心脏的期前收缩与代偿间歇实验
影响心脏活动的化学因素实验
心泵功能的“双信息图”显示实验
人体心率变异性和压力反射敏感性分析实验
大鼠海马神经细胞钠通道电流的记录实验
八臂迷宫实验（Radial Maze）
Y-迷宫实验
穿梭箱实验（TDPA）
穿梭箱实验
旷场实验（OF）
旷场实验
爬杆回避实验
上皮细胞纤毛运动的形态学观察实验
小鼠肥大细胞的形态学观察实验
小鼠睾丸活精子抑制观察实验
细胞的吞噬活动与矽肺发生的形态学观察实验
早期胚胎培养及形态学观察实验
静脉内皮细胞对组胺反应的形态学实验
The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Co
Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identifica
核酶(ribozyme)
细胞培养常见问题及回答
Generation and Establishment of Murine Adherent Cell Lines
Analysis of Myristoylated and Palmitoylated Src Family Proteins
Analysis of Wnt Signaling During Caenorhabditis elegans Postembryonic Development
Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies
In Vivo and In Vitro Models of Mammalian Angiogenesis
Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans
Differentiate ES cells into glial cells and neurons
Cloning of Apoptosis-Related Genes by Representational Difference Analysis of cDNA
High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy
A Molecular Evolution Approach for Isolation of Intrinsically Active (MEK-Independent) MAP Kinases
Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epitheliu
Treatment of Liver Malignancies with Electrochemotherapy in a Rat Model
Bioluminescence Imaging of Human Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Myoc
Chaperone-Mediated Autophagy
Direct DNA Sequencing of PCR Products Using Magnetic Beads
Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells
In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases
Colorimetric Detection of Senescence-Associated Galactosidase
纤维素(cellulose)
Dynamic and Exchange Processes in Macromolecules Studied by NMR Spectroscopy
Biochemical Fractionation of Drosophila Cells
Decontamination of cells from the yeast
Immunohistochemical Analysis of Human Mesenchymal Stem Cells Differentiating into Chondrogenic, Oste
RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis
Analysis of Reconstituted Chromatin Using a Solid-Phase Approach
Terminal Differentiation of Human Epidermal Stem Cells on Micro-patterned Substrates
The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation
Testing Membrane Interactions of CPPs
Manipulating the Cell Differentiation Through Lentiviral Vectors
Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor
基体(basal body)
有关支原体污染的问答
Animal Probes and ZOO-FISH
Generation of Neural Crest Cells and Peripheral Sensory Neurons from Human Embryonic Stem Cells
Platelet Proteomics: State of the Art and Future Perspective
Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells
Mathematical Modeling of Biochemical Systems with PottersWheel
Culture and Preparation of Human Embryonic Stem Cells for Proteomics-Based Applications
In Vitro Reprogramming of Nuclei and Cells
Identification and Relative Quantification of Neuropeptides from the Endocrine Tissues
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID EMBRYOS
捕光色素(light-harvesting pigment)
cell proliferation assay
Quantitative Neuroproteomics of the Synapse
Detecting the Influence of Cell Cycle Regulatory Proteins on Human Telomeres
Analysis of Single Eukaryotic Cells Using Raman Tweezers
Essential Techniques for Introducing Medaka to a Zebrafish LaboratoryTowards the Combined Use of Med
两栖类骨髓细胞染色体制作方法实验
Human Salivary Gland Stem Cells: Isolation, Propagation, and Characterization
踏车现象(treadmilling)
Detection of Apoptosis by TUNEL Assay
Live Microscopy of Neural Stem Cell Migration in Brain Slices
Expression and Refolding of Full-Length HumanTIMP-1
Assays for the Evaluation of HIV-1 Integrase Inhibitors
细胞增殖的检验方法：BrdU标记法
细胞冻存方法
Cryopreservation of Semen from Domestic Livestock
Trypsinizing cells
Flow Cytometric Characterization of Neural Precursor Cells and Their Progeny
Electron Cryomicroscopy of Fibrillar Collagens
Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM Microscopy
Activity-Based Profiling of Lipases in Living Cells
Generation of Differentially Polyglutamylated Microtubules
Suppression of Pancreatic and Colon Cancer Cells by Antisense K-ras RNA Expression VectorsM. Ian Phi
Culture Systems: Low-Oxygen Culture
Peptidomics: Divide et Impera
Visualizing Receptor Endocytosis and Trafficking
ALDH as a Marker for Enriching Tumorigenic Human Colonic Stem Cells
Assaying Chemotaxis of Dictyostelium Cells
卵原细胞(oogonia)
Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis
Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNA
Megakaryopoiesis and Thrombopoiesis: An Update on Cytokines and Lineage Surface Markers
Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice
Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells
Peptide Interaction with and Insertion into Membranes
Detection of TGF in Body Fluids and Tissues
Application of 2D and 3D DIAS to Motion Analysis of Live Cells in Transmission and Confocal Microsco
Determination of Small Ions With Capillary Electrophoresis and Contactless Conductivity Detection
DNA Isolation and Transformation in Escherichia coli: An Introduction
无血清细胞培养基与血清细胞培养基优缺点比较
Identification of Specific Protein/E-Box-Containing DNA Complexes: Lessons from the Ubiquitously Exp
存活因子(survival factors)
Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmentally Regulated Genes in
Photolithographic Patterning of Enzyme Membranes for the Modification of Microelectrodes
Proteomic Analysis of the Left Ventricle Post-myocardial Infarction to Identify In Vivo Candidate Ma
Monitoring Dynamic GPCR Signaling Events Using Fluorescence Microscopy, FRET Imaging, and Single-Mol
Quantitative DNA Fiber Mapping
Acquisition, Display, and Analysis of Digital Three-Dimensional Time-Lapse (Four-Dimensional) Data S
Improved PCR Methods for Identification of Phytopathogenic Viruses
Preparation of Isolated Rat Liver Hepatocytes
起点(START)
Apoptosis in Development
联会复合体的染色与观察实验
Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake
Vitamin D3 Analog Screening
Autophagosome and Phagosome
Nuclear Transfer in Bovine Embryos
Transfer of Eukaryote Suspension Cultures
In Vivo Live-Analysis of Cell Cycle Checkpoints in Drosophila Early Embryos
Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recom
In Vivo Measurements of Cytosolic Calcium in Dictyostelium discoideum
Measurement of Platelet Microparticles
Radio-Isotopic In Situ Hybridization on Tissue Sections: Practical Aspects and Quantification
对付支原体污染的一点经验
Quantitation of Methylmalonic Acid in Serum or Plasma Using Isotope Dilution-Selected Ion Gas Chroma
休克(shock)
Assays for Alloreactive Responses by PCR
Tol2-Mediated Gene Transfer and In Ovo Electroporation of the Otic Placode: A Powerful and Versatile
Hormone-lnducible Genes in Prostate Cells
Multiple Linear Regression
Retroviral Transduction of Murine Hematopoietic Stem Cells
In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells
全血细胞培养染色体技术常用试剂配制
EGF受体(EGF receptor)
Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentia
Generation and Detection of S-Nitrosothiols
Endothelial and Cardiac Regeneration from Adipose Tissues
Measuring Transport and Accumulation of Radiolabeled Substrates in Oocytes and Embryos
Methods for Detection of ProteinProteinnl and ProteinDNA Interactions Using HaloTag
Convergent Extension Analysis in Mouse Whole Embryo Culture
Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine
Thymic Transplantation in Pig-to-Nonhuman Primates for the Induction of Tolerance Across Xenogeneic
Improving Protein Folding Efficiency by Directed Evolution Using the GFP Folding Reporter
SAPK/Jun kinase assays
Superfused Microcarrier Cultures of BeWo Choriocarcinoma Cells: A Dynamic Model for Studies of Human
Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells
Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosectio
Isolation and Characterization of Mouse Side Population Cells
肿瘤发生(tumorigenesis)
Tissue Culture of PtK1 cells
大鼠缺血修饰白蛋白（IMA）酶联免疫分析（ELISA）
细胞学技术的一般环节简介
Analysis of the Fission Yeast Schizosaccharomyces pombe Cell Cycle
Experimental Models to Analyze Differentiation Functions of Cultured Keratinocytes In Vitro and In V
Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes
Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays
Monolithic Bioreactors for Macromolecules
Enrichment of Cells in Different Phases of the Cell Cycle by Centrifugal Elutriation
Three-Dimensional Interphase Analysis Enabled by Suspension FISH
Quantitative Analysis of Protein-RNA Interactions by Gel Mobility Shift
Primary Culture of Human Antral Endocrine Cells
Expression of Mammalian Membrane Proteins in Mammalian Cells Using Semliki Forest Virus Vectors
细胞培养的环境
Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro
Classical Cytogenetics: Karyotyping Techniques
Long-Term Culture of Chicken Blastodermal Cells (CBCs) and Selection of Transfected CBCs Using Antib
Adhesion of Tumor Cells Under Flow
Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelia
Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically Defined Medium wi
淋巴循环的构成元件及其生理/病理形态学观察实验
溶组织内阿米巴形态学观察实验
结肠内阿米巴形态学观察实验
杜氏利什曼原虫形态学观察实验
锥虫形态学观察实验
阴道毛滴虫形态学观察实验
疟原虫形态学观察实验
卡氏肺孢子虫形态学观察实验
肉孢子虫形态学观察实验
隐孢子虫形态学观察实验
结肠小袋纤毛虫形态学观察实验
中华分支睾吸虫形态学观察实验
布氏姜片吸虫形态学观察实验
卫氏并殖吸虫形态学观察实验
日本血吸虫形态学观察实验
异形吸虫形态学观察实验
裂头绦虫形态学观察实验
带绦虫形态学观察实验
细粒棘球绦虫形态学观察实验
短膜壳绦虫形态学观察实验
钩虫形态学观察实验
似蚓蛔线虫形态学观察实验
蠕形住肠线虫形态学观察实验
旋毛形线虫形态学观察实验
美丽筒线虫形态学观察实验
结膜吸吮线虫形态学观察实验
棘腭口线虫形态学观察实验
猪巨吻棘头虫形态学观察实验
淡色库蚊形态学观察实验
中华按蚊形态学观察实验
蝇的形态学观察实验
白蛉形态学观察实验
蚤形态学观察实验
虱形态学观察实验
臭虫形态学观察实验
蠓形态学观察实验
虻形态学观察实验
蚋形态学观察实验
蜚蠊形态学观察实验
蜱形态学观察实验
革螨形态学观察实验
恙螨形态学观察实验
疥螨形态学观察实验
蠕形螨形态学观察实验
结缔组织观察实验
血涂片的制备实验
肌肉组织的观察实验
神经组织观察实验
骨骼形态学观察实验
骨骼肌观察实验
Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR
Evaluation of Immunogenicity of Rat ES-Cell Derived Endothelial Cells
In Vitro Model of Cartilage Degradation
Peptide Assay of Protein Kinases and Use of Variant Peptides to Determine Recognition Motifs
Analysis of Embryonic Stem Cell-Derived Osteogenic Cultures
Preparation of Embryonic Extract
Electroporation and Agrobacterium-Mediated Spore Transformation
Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells
Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
PCR Detection of HIV
细胞学说(cell theory)
Survey Sequencing and Radiation Hybrid Mapping to Construct Comparative Maps
Comparative Gene Mapping in Exotic Species Using FISH
Multivariate Cell Analysis: Techniques for Correlated Measurements of DNA and Other Cellular Constit
ITS-RFLP Matching for Identification of Fungi
Identification of Chemosensory Receptor Genes from Vertebrate Genomes
Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesi
Co-immunoprecipitation Protocol to Investigate Cytokine Receptor-Associated Proteins, e.g., Janus Ki
Electroporation of Plasmid DNA into Normal Human Fibroblasts
Molecular Identification of Phytopathogenic Viruses
Measurement of Protein Tyrosine Phosphorylation in Cell Adhesion
Clonal and Lineage Analysis of Melanocyte Stem Cells and Their Progeny in the Zebrafish
In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells
Role of Claudins in Oxidant-Induced Alveolar Epithelial Barrier Dysfunction
Flexibility in Cell Targeting by Pseudotyping Lentiviral Vectors
Resuscitation of Frozen Cell Lines
Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression
R-带(Reverse-banding)
Transcript End Mapping and Analysis of RNA Editing in Plant Mitochondria
Yeast Cell Synchronization
Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In Vitro
Immobilization in Carrageenans
Analysis of Circadian Leaf Movement Rhythms in Arabidopsis thaliana
Stem Cell Sources for Regenerative Medicine
Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers
Isolation and Differentiation of MonocytesMacrophages from Human Blood
神经细胞粘着分子(neural cell adhesion molecule,N-CAM)
Multiplex FISH and Spectral Karyotyping
Purification of Adipose Tissue Mesenchymal Stem Cells and Differentiation Toward Hepatic-Like Cells
Permeability of Endothelial Barrier: Cell Culture and In Vivo Models
Investigating the Interactions Between Haemopoietic Stem Cells and Their Niche: Methods for the Anal
Flow Cytometric Analysis of Cell Cycle
Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell
Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
Reconstitution of Nuclear Import in Permeabilized Cells
Cellular Characterization of Human Pluripotent Stem Cells
肌原纤维(myofibril)
The Expanding Tool Kit for Hematopoietic Stem Cell Research
Cataloging Proteins in Cell Cycle Control
Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts
Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2
Fluorescence Activated Cell Sorting (FACS)
Evaluation of Individual-Cell Motility
Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension
兔抗胸腺细胞球蛋白(ATG)酶联免疫分析（ELISA）
Monitoring Changes in the Subcellular Location of Proteins in S. cerevisiae
氧化(oxidation)
Flow Cytometric Analysis of Macrophage Oxidative Metabolism Using DCFH
The Plant Cell Cycle: An Overview
大鼠褪黑素 (melatonin)酶联免疫分析
Scalable Production of Embryonic Stem Cell-Derived Cells
Primary Culture of Human Adipocyte Precursor Cells: Expansion and Differentiation
Optical Coherence Tomography Imaging in Developmental Biology
Two-Dimensional Difference Gel Electrophoresis
Digital Imaging of Stem Cells by Electron Microscopy
Lanthanide-Based Upconversion Nanoparticles for Connexin-Targeted Imaging in Co-cultures
细胞学技术的一般环节
DNA Fragmentation Assays for Apoptosis
Polyamine Transport Systems in Mammalian Cells and Tissues
Detection of c-myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue by Chromogenic In
In Vitro and Ex Vivo Gene Delivery to Cells by Electroporation
Introduction: Why Analyze Single Cells
Methods for Homologous Recombination in Drosophila
Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells
Subtelomeric and/or Subcentromeric Probe Sets
Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for th
小鼠重组胰岛素抗体酶联免疫分析（ELISA）
Oocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and Pesticides
Recycling Tubulin
Overview: Imaging in the Study of Integrins
Use of Disulfide Cationic Lipids in Plasmid DNA Delivery
Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab)2, and Streptavidin
Using Drosophila Larval Imaginal Discs to Study Low-Dose Radiation-Induced Cell Cycle Arrest
Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis
Capillary Electrophoresis of Gene Mutation
Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Ti
Viscometric Analysis of DNA-Lipid Complexes
Genetic Analysis of the Vertebrate Hedgehog-Signaling Pathway Using Muscle Cell Fate Specification i
In Vitro Translation Extracts from Tissue Culture Cells
Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells
良性肿瘤(begign tumor)
Measuring Protein Kinase and Sugar Kinase Activity in Plant Pathogenic Fusarium Species
终变期(diakinesis)
常规组织培养法
Perforated Whole-Cell Patch-Clamp Recording
Apoptosis: A Laboratory Manual of Experimental Methods
High-Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant
Methods for Studying the G2 DNA Damage Checkpoint in Mammalian Cells
全能性(totipotency)
Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as P
Identification of Nuclear Receptor Interacting Proteins Using Yeast Two- Hybrid Technology: Applicat
Cytoplasmic Transfer in Mammalian Eggs and Embryos
Transcriptional Regulation of Epidermal Barrier Formation
看护细胞(nurse cell)
胞质溶胶(cytosol)
Industrial-Scale Manufacturing of a Possible Oligonucleotide Cargo CPP-Based Drug
Wnt Signaling in Cnidarians
培养细胞中RNA检测－Northern Blot
Synthesis and Application of Peptides as Drug Carriers
The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cyt
Dictyostelium discoideum as a Model to Study Host-Pathogen Interactions
Analysis of GPCR/Ion Channel Interactions
Assessment of White Adipose Tissue Metabolism by Measurement of Arteriovenous Differences
细胞凋亡与肝
Cell Surface Markers in Human Embryonic Stem Cells
Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stem
Migration Assay Protocol:
循环系统观察实验
消化系统的大体解剖结构实验
消化系统的显微结构观察实验
呼吸系统观察实验
泌尿系统观察实验
内分泌系统观察实验
感觉器与皮肤解剖观察实验
脊髓的构造与脑干的外形观察实验
脑干的内部结构观察实验
间脑、小脑与端脑解剖观察实验
尸体的收集、消毒、防腐、固定和保存实验
解剖操作的基本实验
实验动物接种途径和接种方法试验-颅内接种法
实验动物接种途径和接种方法试验-消化道接种法、鼻腔接种法及角膜接种法
实验动物接种途径和接种方法试验-静脉接种
Analysis of Apoptosis in Isolated Primary Cardiac Myocytes
细胞化学(cytochemistry)
Developmental Biology Protocols: Overview III
Approaches to Detect PARP-1 Activation In Vivo, In Situ, and In Vitro
Leukocyte Transmigration Through Vascular Endothelium: An In Vitro Method
Detection and Isolation of Human Disseminated Tumor Cells in the Murine Bone Marrow Stem Cell Niche
Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse
In Vivo Germ Line Stem Cell Migration: A Mouse Model
Using Electrostatics to Define the Active Site of Serratia Endonuclease
Analysis of Schizosaccharomyces pombe Meiosis by Nuclear Spreading
Tight Junctions and CellCell Interactions
Human Trials for Neurodegenerative Disease
Embryological and Genetic Manipulation of Chick Development
Genetic Dissection of Caenorhabditis elegans Embryogenesis Using RNA Interference and Flow Cytometry
Chick Spinal Somatic Motoneurons in Culture
Patterned Polymeric Surfaces to Study the Influence of Nanotopography on the Growth and Differentiat
Isolation and Characterization of Resident Mesenchymal Stem Cells in Human Glomeruli
Detection of microRNAs in Plants by In Situ Hybridisation
Microemulsion Electrokinetic Chromatography
Fluorescence Imaging of Osteoclasts Using Confocal Microscopy
Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
Em observations of microsomes
An NMR Method to Study ProteinProtein Interactions
Measurement of Oxidized and Reduced Coenzyme Q in Biological Fluids, Cells, and Tissues: An HPLC-EC
Cryopreservation of cell cultures
实验动物接种途径和接种方法试验-皮下接种法及腹腔接种法
实验动物实验标本的采集实验-实验动物腹水的采集
实验动物实验标本的采集实验-实验动物尿液采集
实验动物实验标本的采集实验-实验动物脑脊液采集
实验动物实验标本的采集实验-动物采血方法
动物的注射方法实验-静脉注射法
动物的注射方法实验-皮内注射法
动物的注射方法实验-腹腔注射法
动物的注射方法实验-皮下注射法
水迷宫实验（MWM）
Identification of ImmediateEarly Gene Targets of the Raf-1 Serine/Threonine Protein Kinase Using an
Morris水迷宫实验
Comparison of Means
肝脏药物代谢酶CYP450含量测定实验
Cytological Analysis of Meiosis in Caenorhabditis elegans
In Vitro Maturation and Fertilization of Pig Oocytes
Cloning Technologies
药物半数致死量LD50的测定实验
Non-invasive Mapping of Lipids in Plant Tissue Using Magnetic Resonance Imaging
成年小鼠空间学习记忆的检测 Morris 水迷宫实验-Y 迷宫实验（Y-Maze）
Detecting and Quantifying pADPr In Vivo
Primary Culture of the Human Olfactory Neuroepithelium
成年小鼠空间学习记忆的检测 Morris 水迷宫实验-Morris水迷宫
Analysis of PCP Defects in Mammalian Eye Lens
小鼠脑片免疫组织化学（ABC 法）实验
Activation of Ras and Rho GTPases and MAP Kinases by G-Protein-Coupled Receptors
Methods to Validate Tooth-Supporting Regenerative Therapies
药物对动物体内 NK 细胞活性的影响实验-瘤细胞悬液皮下接种法
Ras Proteins Signaling in the Early Metazoan Dictyostelium discoideum
肠道菌群检测实验
Supramolecular Self-Assembly of Poly(ethylene glycol)-Block-Poly(L-lysine) Dendrimer with Plasmid DN
血清及血浆制备实验
Analysis of Connexin 43 Expression on Keratinocytes Using Flow Cytometry
血液检测实验-血涂片
Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System
肠道免疫评价实验-流式细胞分析法评价肠道免疫
Silver Staining of Proteins in 2DE Gels
Genome-Wide Analysis of c-MYC-Regulated mRNAs and miRNAs, and c-MYC DNA Binding by Next-Generation S
肠道免疫评价实验-ELISA法评价肠道免疫功能
Flow Cytometry as a Tool for Measurement of Steroid Hormone Receptor Protein Expression in Leukocyte
肠道通透性检测实验
The Derivation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation
小鼠外表观察实验
Detection of Small RNAs and MicroRNAs Using Deep Sequencing Technology
粪尿收集实验
植物染色体的组型分析
Measuring Size-Dependent Permeability of the Tight Junction Using PEG Profiling
Clonogenic Assays for Hematopoietic and Tumor Cells Using Agar-Containing Capillaries
Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP
Quantification of Xenoprotein Electroinsertion in Mammalian Cells
Wound healing assay
Imaging of Transcription Factor Trafficking in Living Cells: Lessons from Corticosteroid Receptor Dy
Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles
Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers
Tracking Gene-Modified T Cells In Vivo
消化系统组织学分析实验-免疫组化（荧光）确定上皮增生及细胞浸润
消化系统组织学分析实验-切片观察实验
小鼠采血实验-腹主动脉采血法
小鼠采血实验-颌下静脉采血法
小鼠采血实验-眼后静脉采血法
小鼠采血实验-尾静脉采血法
小鼠体温测量实验-直肠肛温测量小鼠体温
血管插管术
气管插管术
动物实验外科手术吻合术-神经吻合术
动物实验外科手术吻合术-肌腱吻合术
动物实验外科手术吻合术-肠吻合术
动物实验外科手术吻合术-小血管吻合术
免疫组化（荧光）实验
小鼠常规病理分析实验-石蜡包埋切片
小鼠常规病理分析实验-组织分离及固定
小鼠常规病理分析实验-灌流
增殖凋亡分析实验-TUNEL 检测凋亡实验
增殖凋亡分析实验-BrdU 掺入及增殖检测实验
小鼠卵巢组织切片 HE 染色
HE 染色实验
卵母细胞冷冻保存技术快速冷冻法
卵母细胞冷冻保存技术慢速冷冻法
冻存胚胎、精子、卵巢的复苏
大鼠卵巢冻存保存方法
大鼠精子冻存保存方法
大鼠胚胎冻存保存方法
常规观察实验-瘦肉和脂肪组成检测
常规观察实验-间接法测热量开支
胰岛素功能相关检测实验-分离胰岛的糖刺激下胰岛素分泌检测
胰岛素功能相关检测实验-小鼠胰岛的分离纯化实验
胰岛素功能相关检测实验-胰岛素耐受实验
胰岛素功能相关检测实验-腹腔或口服葡萄糖耐量试验
雌性小鼠生殖生物学分析实验-卵细胞体外受精、发育实验
雌性小鼠生殖生物学分析实验-卵母细胞体外成熟实验
雌性小鼠生殖生物学分析实验-各时期卵泡数量统计实验
雌性小鼠生殖生物学分析实验-超数排卵实验
雌性小鼠生殖生物学分析实验-血清激素检测实验（雌性小鼠生殖生物学分析实验）
雌性小鼠生殖生物学分析实验-生育力检测实验
鼻咽癌动物模型建立-二乙基亚硝胺 (DEN) 法诱发鼻咽癌
鼻咽癌动物模型建立-二甲基胆蒽 (MC) 法诱发鼻咽癌
肺癌动物模型建立-肺癌动物模型
肺癌动物模型建立-气管内注入苯并花、硫酸铵气溶胶、甲基胆蒽等物质法构建肺癌动物模型
肺癌动物模型建立-焦油沥青 (GTP) 法构建肺癌动物模型
Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria
Methods for Assessing Autophagy and Autophagic Cell Death
Cultivation of Normal Human Epidermal Melanocytes
Isolation and Characterization of Human Embryonic Stem Cells
The Application of X-Ray Absorption Spectroscopy to Characterize Metal Centers in Proteins
Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration
Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates
Use of Multiphoton Imaging for Studying Cell Migration in the Mouse
Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromato
程序性细胞死亡(programmed cell death,PCD)
细胞培养常见问题及其解决方法[3]
Assays of Glucose Entry, Glucose Transporter Amount, and Translocation
Large Scale Production of Nuclear Receptor Ligand-Binding Domains
Differentiation and Lineage Commitment of Murine Embryonic Stem Cells into Insulin Producing Cells
Chemotropism During Yeast Mating
Identification of Interacting Proteins Using the Yeast Two-Hybrid Screen
Identification of p53-Regulated Genes by the Method of Differential Display
Adhesion Assay Protocol
细胞系数相关资料
Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells
Techniques for Thick-Section Golgi Impregnation of Formalin-Fixed Brain Tissue
Radioactive Labeling of Mitochondrial Translation Products in Cultured Cells
Tumorcytogenetic Diagnostics and Research on Uncultured Blood or Bone Marrow and Smears
Estimation of Drug Resistance by Flow Cytometry
Freeze-Drying of Yeasts
Measurement of Diacylglycerol: Origins of Receptor- Stimulated Diacylglycerol Accumulation
Reporter Assays
Electroporation of Rat Pituitary Cells
Immunoassay for Collagenase-Mediated Cleavage of Types I and II Collagens
Establishment of a Primary Culture
Flow Cytometric Analysis of STAT Phosphorylation
Isolation, Characterization, and Differentiation of Human Multipotent Dermal Stem Cells
Digital Image Processing of Live/Dead Staining
The Analysis and Assay of Cyclic Nucleotide Phosphodiesterase Isoenzyme Activity
Analysis of Molecular Markers for Staging Peri-gastrulating Bovine Embryos
大鼠心脏纤维原细胞（HCF)酶联免疫分析
Evaluation of Retroviral Ribonuclease H Activity
Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells
Urine Organic Acid Analysis for Inherited Metabolic Disease Using Gas Chromatography-Mass Spectromet
Analysis of Gene Expression in Hypothalamus in Obese and Normal Mice Using Differential Display
MEF小鼠胚胎成纤维细胞富集/消化/传代知识总结
In Vitro PhagosomeEndosome Fusion
The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF
Structure of Mitochondria
Atomic Force Microspopy Measurements of Intermolecular Binding Strength
11 Detection of TGF Type II Receptor Hotspot Mutations: The BAT-RII Assay
Computational Modelling of Kinase Signalling Cascades
食管癌动物模型建立-二氨黄樟素 (dihydrosafrole) 法诱发食管癌
Boyden Chamber
Urinary Proteomics Employing Capillary Electrophoresis Coupled to Mass Spectrometry in the Monitorin
多聚赖氨酸实验中经典问题分析
食管癌动物模型建立-甲基苄基亚硝胺 (MBNA) 法诱发食管癌
血清学检测实验-激素水平检测实验
移植瘤动物模型的建立
大鼠卵巢移植
大鼠胚胎移植
人体异种移植性肿瘤动物模型的建立
制备转基因肿瘤动物
组织学分析实验-TRAP 染色实验
组织学分析实验-骨组织脱钙（快速、慢速）实验
组织学分析实验-小鼠骨骼标本制作及双色染色
新生儿胎粪吸入综合征动物模型的建立
新生儿胆红素脑病动物模型的建立
大鼠嵌合体制备技术-ES细胞显微注射法制作嵌合体大鼠
大鼠嵌合体制备技术-聚集法制作嵌合体大鼠
新生儿缺氧缺血性脑病动物模型的建立
Cell Labeling and Gene Misexpression by Electroporation
Methods to Detect Patterns of Cell Death in Drosophila
Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues
The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes
Assaying Phospholipase A2 Activity
Analyzing p53 Regulated DNA Damage Checkpoints by Flow Cytometry
Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes
Permeability of Differentiated Human Urothelium In Vitro
The Use of Permeabilized Cell Systems to Study Nuclear Transport
Fluorescence Correlation Spectroscopy for the Study of Membrane Dynamics and Organization in Giant U
Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots
Genetic and Biochemical Analysis of Protozoal Polyamine Transporters
In Vitro Nanoparticle-Mediated Intracellular Delivery into Human Adipose-Derived Stem Cells
Micropatterning Cell Adhesion on Polyacrylamide Hydrogels
Tissue Engineering of Cartilage
Craniofacial Development and Patterning
培养用品的清洗与消毒
Assays for the Measurement of Lipid Peroxidation
Analysis of Muscle Gene Transcription in Cultured Skeletal Muscle Cells
核基质(nuclear matrix)
Protein/Peptide Transduction in Metanephric Explant Culture
Cartilage Tissue Engineering: Directed Differentiation of Embryonic Stem Cells in Three-Dimensional
Ligand-Regulated Peptide Aptamers
Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors
Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment, and Following Living Cells in
Isolation and Culture of Embryonic Pancreas and Liver
细胞培养用滤网的基本知识
Interphase FISH: Detection of Intercellular Genomic Variations and Somatic Chromosomal Mosaicism
Differentiation of Adipose-Derived Stem Cells for Tendon Repair
Analysis of Telomeres and Telomerase
Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase
Chromosomal Double-Strand Breaks Introduced in Mammalian Cells by Expression of I-Sce I Endonuclease
A Brief Introduction to RNAi and MicroRNAs in Stem Cells
Microscopic Computed Tomography-Based Skeletal Phenotyping for Genetic Model Organisms
In Vitro Differentiation of Embryonic Stem Cells Into the Pancreatic Lineage
Effects of Altered Gene Expression on ES Cell Differentiation
Measuring the Action of CPPsiRNA Conjugates in the Lung
Detection of Human Papillomavirus DNA in Sperm Using Polymerase Chain Reaction
RNAi in Stem Cells: Current Status and Future Perspectives
Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization
Surface Plasmon Resonance Biosensing in Studies of the Binding Between 2 Integrin I Domains and Thei
Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos
人肝细胞生长因子（HGF）酶联免疫分析（ELISA）
Conversion of Non-endocrine Human Pancreatic Cells to Insulin-Producing Cells for Treatment of Diabe
Engineered Properties and Assays for Human DNase I Mutants
Current Status of the Polyamine Research Field
Electroporation of Muscle Tissue In Vivo
Amphibian Organizer Activity
Cell Senescence Culturing Methods
Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
绝赞二抗使用指南
Mitophagy and Mitoptosis in Disease Processes
Expression of I-Sce I in Drosophila to Induce DNA Double-Strand Breaks
Ex Vivo Expansion of Human and Murine Hematopoietic Stem Cells
Functional Analysis by Inducible RNA Interference in Drosophila melanogaster
我对付支原体污染的一点经验
In Vivo Labeling and Analysis of Epidermal Stem Cells
Small-Angle X-Ray Scattering Studies of Membrane Transporter Peripheral Components and Soluble Domai
Selective Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage
Scanning Electron Microscopy of Bone
Structural Studies on MMPs andTIMPs Wolfram Bode and Klaus Maskos
脐带内皮细胞的分离
Specialized Techniques for Site-Directed Mutagenesis in Cyanobacteria
阻抗与射频技术联合分类法
Recipes for Tissue Culture Selective Drug Stocks
Tissue Culture - Storage of Cell Lines
Chloramphenicol Acetyltransferase as a Reporter in Mammalian Gene Transfer
Epithelial Differentiation of Human Adipose-Derived Stem Cells
Isolation of Muscle-Derived Stem/Progenitor Cells Based on Adhesion Characteristics to Collagen-Coat
Using the mRNA-MS2/MS2CP-FP System to Study mRNA Transport During Drosophila Oogenesis
Simultaneous Determination and Quantification of 12 Benzodiazepines in Serum or Whole Blood Using UP
Detection of Intracellular/lntranuclear Antigens in Tumor Cells
Bioinformatics and Data Integration in Membrane Transporter Studies
In Vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins
Stabilization and Electrophoretic Analysis of Meiotic Recombination Intermediates in Saccharomyces c
Tracing the Endocytosis of Claudin-5 in Brain Endothelial Cells
Manipulating Dynein Genes in Tetrahymena
Necrosis in C. elegans
Modification of Carbon Nanotube Electrodes with 1-Pyrenebutanoic Acid, Succinimidyl Ester for Enhanc
Expression of Recombinant Matrix Metalloproteinases in Yeast
Urinary Oxalate and Citrate
Primary Cultures of Sympathetic Ganglia
RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues
Capillary Electrophoresis of Tropane Alkaloids and Glycoalkaloids Occurring in Solanaceae Plants
Assessing Vascular Senescence in Zebrafish
Detection of Apoptosis in Cell-Free Systems
The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cel
Noninvasive Imaging of Myocardial Blood Flow Recovery in Response to Stem Cell Intervention
Isolation and Maintenance of Mouse Epiblast Stem Cells
Broad Spectrum Drug Screening Using Liquid Chromatography-Hybrid Triple Quadrupole Linear Ion Trap M
Nuclear Remodeling Assay in Xenopus Egg Extract
Reconstitution of the Central Nervous System During Salamander Tail Regeneration from the Implanted
Magnetic Resonance Imaging of Stem Cell Migration
Functional Development of Human Fetal Gastrointestinal Tract
Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry
Studying the Regulation of MAP Kinase by MAP Kinase Phosphatases In Vitro and in Cell Systems
Free-Flow Electrophoresis System for Proteomics Applications
Human Peripheral Blood Mononuclear Cell Culture for Flow Cytometric Analysis of Phosphorylated Mitog
Protein Aggregation Diseases: Toxicity of Soluble Prefibrillar Aggregates and Their Clinical Signifi
微绒毛(microvilli)
Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins
什么是高纯度化学试剂等级
Live Cell Imaging of Kinetochore Capture by Microtubules in Budding Yeast
Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis
Elimination of Cell Types from Mixed Neural Cell Cultures
大鼠甲状腺素(T4)酶联免疫分析
Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Diff
Switching on Lineage Tracers Using Site-Specific Recombination
cdc25和chk1与细胞凋亡
Osteoblast Harvest(Adult Mice)
Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plate
Rapid Analysis of Amplified Double-Stranded DNA by Capillary Electrophoresis with Laser-Induced Fluo
Isolation of Plasmid DNA: The Cleared Lysate and CsCl Gradient Technique
细胞分裂素信号转导的分子遗传学机理
Adhesion of Tumor Cells to Endothelium Under Static Conditions
人可溶性白细胞抗原G(sHLA-G)酶联免疫分析（ELISA）
Direct Differentiation of Human Embryonic Stem Cells into Selective Neurons on Nanoscale Ridge/Groov
有丝分裂（Feulgen染色法）
Electroporation of ES cells
Staining protocols for placental explant cultures
Establishment of a Primary Culture
Isolation of Stem Cells from Human Umbilical Cord Blood
Methods to Study Cancer Therapeutic Drugs That Target Cell Cycle Checkpoints
Ordered Deletions Using Exonuclease III
Chromatin Immunoprecipitation (ChIP) Methodology and Readouts
小鼠巨噬细胞炎性蛋白1α(MIP-1α)酶联免疫分析
Mycoplasma Detection
Skin Stem Cells
Exo utero Surgery
Flow Cytometric Studies on Intracellular Drug Fluorescence
次缢痕(secondary constriction)
新型培养基：大批量生产胚胎干细胞新方法
Time-Lapse Cinemicroscopy
细胞组分和细胞器――线粒体
Stem Cell Migration: A Zebrafish Model
Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging
值得推荐的血涂片制备及白细胞分类计数方法与步骤
Cryopreservation of Semen of the Salmonidae with Special Reference to Large-Scale Fertilization
FISH on Insect Cells Transfected with Heterologous DNA: A Single-Cell-Directed Approach
RNAi in Drosophila S2 Cells as a Tool for Studying Cell Cycle Progression
Detection of Kinase and Phosphatase Activities
Random Sequence Mutagenesis for the Generation of Active Enzymes
Digital Image Analysis of Cells Stained with the Senescence-Associated -Galactosidase Assay
Introduction to Pichia pastoris
Isolation of Human Adipose-derived Stem Cells from Biopsies and Liposuction Specimens
Use of Pichia pastoris as a Model Eukaryotic System: Peroxisome Biogenesis
有色体(chromoplast)
Subnuclear Localization and Intranuclear Trafficking of Transcription Factors
On-Column Ligand/Receptor Derivatization Coupled to Affinity Capillary Electrophoresis
HIV-1-Induced Alterations of Claudin-5 Expression at the BloodBrain Barrier Level
Autophagosomes in GFP-LC3 Transgenic Mice
Profiling MicroRNAs by Real-Time PCR
细胞培养过程中的注意事项及试剂配制
Determination of IC50
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
Methylation in Embryonic Stem Cells In Vitro
Xenoantibodies and Complement Activity Determinations in Pig-to-Primate Xenotransplantation
DCs in Mouse Models of Intracellular Bacterial Infection
Using Total Internal Reflection Fluorescence Microscopy to Observe Ion Channel Trafficking and Assem
Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield
Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Prog
The Cranial Bone Window Model: Studying Angiogenesis of Primary and Secondary Bone Tumors by Intravi
Identification and Quantitation of Zolpidem in Biological Matrices Using Gas Chromatography-Mass Spe
Interactions Between Clostridium perfringens Enterotoxin and Claudins
Analysis of Gene Expression in Xenopus Embryos
Derivation and Characterization of Lentoid Bodies and Retinal Pigment Epithelial Cells From Monkey E
Immunolabeling of Imaginal Discs
Skin-Derived Mesenchymal Stem Cells: Isolation, Culture, and Characterization
Fluorescence Assays to Study Cell Adhesion and Migration In Vitro
Electroporation of Human Lymphoblastoid Cells
Motility Assay
细胞凝集反应实验
GAL4/UAS Targeted Gene Expression for Studying Drosophila Hedgehog Signaling
A Two- and Three-Dimensional Approach for Visualizing Human Embryonic Stem Cell Differentiation
An Escherichia coli-Based Genetic Strategy for Characterizing RNA Binding Proteins
Single Cell Gene Expression Analysis of Pluripotent Stem Cells
Media Composition: Energy Sources and Metabolism
Identification of Cellular MMP Substrates Using Quantitative Proteomics: Isotope-Coded Affinity Tags
Angiogenesis Assays Using Chick Chorioallantoic Membrane
Expression and Assay of Recombinant ATM
Production of Transgenic and Knockout Pigs by Somatic Cell Nuclear Transfer
Cytological Analysis of Arabidopsis thaliana Meiotic Chromosomes
Internet-Based Sequence-Typing Databases for Bacterial Molecular Epidemiology
UV Spectrophotometric Analysis of Ribonucleic Acids
Virus Cryopreservation and Storage
Differentiate ES cells into glial cells and neurons
In Situ Hybridization to RNA in Whole Embryos
大鼠前列腺素Ｅ２（PGE2）酶联免疫分析
粘合连接（adheringjunction）
Long-Term Culture and Coculture of Primary Rat and Human Hepatocytes
Studying MAP Kinase Pathways During Early Development of Xenopus laevis
An Efficient Protocol for DNA Amplification of Multiple Amphibian Skin Antimicrobial Peptide cDNAs
Superparamagnetic Iron Oxide Labeling of Stem Cells for MRI Tracking and Delivery in Cardiovascular
Image Cytometry: Nuclear and Chromosomal DNA Quantification
Aseptic Technique and Good Cell Culture Practice
Trafficking of Stem Cells
Comparison of Molecular Typing Methods Applied to Clostridium difficile
Neurulation and Neural Tube Closure Defects
Complexation of siRNA and pDNA with Cationic Liposomes: The Important Aspects in Lipoplex Preparatio
Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos
Isolation and Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Hum
早产儿视网膜病变动物模型的建立
坏死性小肠结膜炎动物模型的建立
流式细胞术检测实验-流式细胞术检测胎肝细胞凋亡
流式细胞术检测实验-流式细胞术检测检测胎肝 Lin-c-Kit+Sca-1+细胞数
X 线分析实验
大鼠体细胞核移植技术
细胞的集落形成实验
斑马鱼遗传修饰技术-Tol2转座子介导基因转移技术修饰斑马鱼基因
斑马鱼遗传修饰技术-显微注射质粒DNA技术修饰斑马鱼基因
斑马鱼遗传修饰技术-Morpholino修饰斑马鱼基因
骨密度分析实验
创伤愈合实验
小鼠皮肤组织学分析实验-小鼠尾部皮肤的表皮毛囊全标本（whole mount）的标记保留细胞（LRCs）检测
小鼠皮肤组织学分析实验-小鼠尾部皮肤的表皮毛囊全标本（whole mount）的免疫荧光染色实验
小鼠皮肤组织学分析实验-小鼠尾部皮肤的表皮毛囊全标本（wholemount）获得实验
小鼠皮肤组织学分析实验-小鼠皮脂腺的油红染色实验
小鼠皮肤组织学分析实验-小鼠皮肤冷冻包埋实验
新型支气管肺发育不良动物模型的建立
静脉置管法建立静脉置管感染动物模型
气管导管相关性细菌生物膜感染动物模型的建立
情绪状态筛选实验-开场实验
维生素 A 缺乏动物模型的建立
维生素 D 缺乏动物模型的建立
食物过敏动物模型-BN 大鼠制备食物过敏模型
食物过敏动物模型-BALB/c 小鼠制备食物过敏模型
比较基因组常用分析方法-分子系统发生分析
比较基因组常用分析方法-多序列比对
比较基因组常用分析方法-序列比对
斑马鱼精子冻存与复苏技术-斑马鱼精子复苏技术
斑马鱼精子冻存与复苏技术-斑马鱼精子冻存技术
社会行为能力检测实验-小鼠的社会等级实验
社会行为能力检测实验-小鼠的三箱社交
社会行为能力检测实验-食物偏向的社会性改变实验
社会行为能力检测实验-主导性钻管实验
眼部疾病表型分析实验-检眼镜检测视网膜变性
眼部疾病表型分析实验-裂隙灯显微镜检测眼部疾病
神经肌肉功能检测实验-SHIRPA 检测实验
神经肌肉功能检测实验-抓力测定实验
听觉生理功能测试实验-耳声发射测试
听觉生理功能测试实验-听性脑干反应检测
动物胚胎冷冻保存技术-动物胚胎冷冻保存技术玻璃化法
动物胚胎冷冻保存技术-动物胚胎冷冻保存技术一步冷冻法
动物胚胎冷冻保存技术-动物胚胎冷冻保存技术快速冷冻法
动物胚胎冷冻保存技术-动物胚胎冷冻保存技术常规缓慢冷冻法
神经生物学基本组织学检测实验-振荡切片
神经生物学基本组织学检测实验-尼氏染色法检测神经细胞功能
口腔疾病动物模型-正畸牙移动动物模型
口腔疾病动物模型-牙周病动物模型
口腔疾病动物模型-龋病动物模型
口腔疾病动物模型
雄性小鼠生育力检测实验-血清激素检测实验（雄性小鼠生育力检测实验）
雄性小鼠生育力检测实验-流式细胞仪检测睾丸细胞悬液 DNA 含量
雄性小鼠生育力检测实验-睾丸组织细胞形态学检测实验
雄性小鼠生育力检测实验-附睾精子评估实验
雄性小鼠生育力检测实验-交配生育实验
雄性小鼠生育力检测实验-雄性生殖系统发育分析实验
骨髓细胞移植实验
小鼠气道哮喘模型的建立及分析实验-有创式flexiVent体描系统检测气道生理特性
小鼠气道哮喘模型的建立及分析实验-小鼠气道哮喘模型炎症分析实验
小鼠气道哮喘模型的建立及分析实验-气道应答性分析实验
小鼠气道哮喘模型的建立及分析实验-小鼠呼吸道哮喘模型建立实验
耳的形态学检测实验-锇酸染色法
支气管长张力测定实验
小鼠心脏超声波检查实验
小鼠心脏传导和心律失常检测实验-心电图分析小鼠心率
血压测定实验-尾袖法检测小鼠血压
猪卵母细胞的获得
小鼠血管张力测量实验-610M多通道血管张力测定仪测量小鼠血管张力
心血管系统组织学分析实验-油红 O 染色检测血管内脂质沉淀
心血管系统组织学分析实验-Masson 三色染色法检测心肌纤维化
心血管系统组织学分析实验-TTC 染色法检测心肌梗死
猪卵母细胞冻存保存方法
猪精子冻存保存方法
猪胚胎冻存保存方法
小鼠胚胎泌尿系统体外培养实验
小鼠胚胎泌尿系统获取实验
学习认知能力检测实验-Morris 水迷宫检测实验
学习认知能力检测实验-T 形迷宫或 Y 形迷宫检测实验
胚胎期生殖系统检测实验-生殖嵴碱性磷酸酶染色
家兔胚胎冷冻技术
中央处理感官信息能力检测实验-监测昼夜节律实验
中央处理感官信息能力检测实验-前脉冲抑制的惊吓反应检测实验
家兔胚胎移植技术
家兔配子和卵巢冷冻保存技术-家兔卵巢的冷冻保存
家兔配子和卵巢冷冻保存技术-家兔精液的冷冻保存
家兔配子和卵巢冷冻保存技术-家兔卵母细胞的冷冻保存
家兔嵌合体技术
家兔体外受精技术
猪胚胎移植技术
家兔体细胞核移植技术
猪体外受精技术
家兔性别控制技术
胚胎分割
猪显微受精技术
体细胞核移植
猪性别控制技术
显微受精技术
哺乳动物的胚胎移植
囊胚来源ES细胞系的分离和培养
哺乳动物的体外受精
着床前小鼠胚胎的获取与培养
猪体内胚胎的获取
勃起功能障碍动物模型-内分泌性 ED 模型
勃起功能障碍动物模型-肝气郁结症小鼠 ED 模型
勃起功能障碍动物模型-血管性 ED 模型
肠出血性大肠埃希菌动物模型-猪感染模型-肠出血性大肠埃希菌动物模型
肠出血性大肠埃希菌动物模型-小鼠感染模型-肠出血性大肠埃希菌动物模型
肠出血性大肠埃希菌动物模型-兔感染模型-肠出血性大肠埃希菌动物模型
肠出血性大肠埃希菌动物模型-灵长类哺乳动物感染模型-肠出血性大肠埃希菌动物模型
肠出血性大肠埃希菌动物模型-鸡感染模型-肠出血性大肠埃希菌动物模型
肠出血性大肠埃希菌动物模型-反刍动物感染模型-肠出血性大肠埃希菌动物模型
隔离器化学灭菌技术-过氧化氢气体隔离器灭菌法
隔离器化学灭菌技术-二氧化氯气体隔离器灭菌法
隔离器化学灭菌技术-甲醛气体隔离器灭菌法
隔离器化学灭菌技术-过氧化氢和过氧乙酸隔离器灭菌法
隔离器化学灭菌技术-二氧化氯隔离器灭菌法
隔离器化学灭菌技术-过氧乙酸隔离器灭菌法
精索静脉曲张动物模型的建立
去势动物模型的建立
隔离器附属物灭菌技术-隔离器附属物放射灭菌法
隔离器附属物灭菌技术-隔离器附属物蒸汽灭菌法
隐匿阴茎动物模型
隔离器气密性检测技术
隔离器微生物污染检测技术-隔离器病毒污染血清检测法
隔离器微生物污染检测技术-隔离器污染微生物检测法
隔离器微生物污染检测技术-隔离器污染粪便镜检法
乙型肝炎动物模型-非人灵长类动物模型
尿道下裂动物模型
无菌小鼠剖宫产-子宫无菌切除术
无菌小鼠人工哺乳-自动哺乳
无菌小鼠人工哺乳-吮吸法人工哺乳
无菌小鼠人工哺乳-强饲法人工哺乳
无菌小鼠代乳及代孕技术-无菌小鼠代孕技术
无菌小鼠代乳及代孕技术-无菌小鼠代乳技术
小鼠胚胎干细胞的鉴定-特异标记免疫荧光实验鉴定小鼠干细胞
小鼠胚胎干细胞的鉴定-AKP染色鉴定小鼠干细胞
小鼠胚胎干细胞的鉴定-核型分析鉴定小鼠干细胞
小鼠胚胎干细胞的鉴定-形态学观察鉴定小鼠干细胞
金黄色葡萄球菌感染动物模型-金黄色葡萄球菌致心内膜炎模型
金黄色葡萄球菌感染动物模型-金黄色葡萄球菌致败血症和脓毒血症动物模型
金黄色葡萄球菌感染动物模型-金黄色葡萄球菌 L 型致间质性肺炎动物模型
铜绿假单胞菌感染动物模型-铜绿假单胞菌感染大鼠肺炎模型
铜绿假单胞菌感染动物模型-铜绿假单胞菌感染小鼠肺炎模型
铜绿假单胞菌感染动物模型-铜绿假单胞菌感染家兔肺炎模型
显微注射技术制备转基因动物-利用显微注射技术制备转基因兔
显微注射技术制备转基因动物-利用显微注射技术制备转基因小鼠
体细胞核移植技术制备转基因动物-利用体细胞核移植技术制备转基因猪
胚胎移植-细菌人工染色体转基因动物技术
通过重组逆转录病毒（慢病毒）载体感染制备转基因动物
基于胚胎干细胞同源重组的基因敲除技术
神经组织细胞培养
Analysis of Testosterone in Serum Using Mass Spectrometry
Differentiation of Neuroepithelia from Human Embryonic Stem Cells
Analysis of Male Aggressive and Sexual Behavior in Mice
Affinity Processing of Cell-Containing Feeds Using monolithic Macroporous Hydrogels, Cryogels
The Physiology of the Xenopus laevis Ovary
Adsorption of Lipase on Inorganic Supports
Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template
Isotype Determination of Monoclonal Antibodies by Immunodiffusion
ORGANOTYPIC KIDNEY CULTURES
Determination of Acrolein by High-Voltage Capillary Electrophoresis from Oxidized Fatty Acids
Cloning and Expression Analyses of Pig Genes
Directed Differentiation of Human Embryonic Stem Cells to Dendritic Cells
Growth of Human Embryonic Stem Cells in Long-Term Hypoxia
Plasma Nitrite and Nitrate Determination
Analysis of Histone Posttranslational Modifications from Nucleolus-Associated Chromatin by Mass Spec
Determination of Polyamines by Capillary Electrophoresis
染色体早熟凝集(premature chromosome condensation,PCC)
Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Preparation of cytoplasmic extracts for the application in a cell-free system
Differentiate ES cells into cardiac myocytes
无菌操作技术(细胞培养)
Introduction to Cell Culture
Reptile Embryology
倒置显微镜的操作程序及日常维护需知
Routine Culturing of ES Cells
Quantitative Determination of Collagen Cross-links
形态发生素(morphogen)
Transplantation Chimeras: Use in Analyzing Mechanisms of Avian Development
Telomerase RNA Inhibition Using Antisense Oligonucleotide Against Human Telomerase RNA Linked to a 2
In Vitro and In Vivo Assays of B-Lymphocyte Migration
Lipid Raft-Redox Signaling Platforms in Plasma Membrane
Mouse ES Cell-Derived Hematopoietic Progenitor Cells
Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells
Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microsco
桥粒(desmosomes)
Classes and Prediction of Cell-Penetrating Peptides
Extraction of Nucleic Acids from Bone
Scale-Up of Single CellInoculated Suspension Cultures of Human Embryonic Stem Cells
Preparation of Chick Striated Muscle Cultures
Regulator of Epidermal Growth Factor Signaling: Sprouty
NK细胞介导的细胞溶解作用的研究
Chemiluminescence Assays to Investigate Membrane Expression and Clathrin-Mediated Endocytosis of KAT
Use of Murine Embryonic Stem Cells in Embryotoxicity Assays: The Embryonic Stem Cell Test
Isolation and Characterization of Side Population Cells
Mammalian Embryo Culture in a Microfluidic Device
Analyzing the Roles of Rho GTPases in Cancer Cell Migration with a Live Cell Imaging 3D-Morphology-B
Isolation of Intact, Functional Mitochondria From the Model Plant Arabidopsis thaliana
Quantification of Protein Complexes by Blue Native Electrophoresis
Expression of MMPs andTIMPs in Mammalian Cells
萤光素酶载体PGL3转染细胞到底要不要加G418
Characterization of RNAProtein Interactions by Phosphorothioate Footprinting and Its Applications to
Isolation and Culture of Rodent Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells
Imaging the Cytoskeleton in Live Xenopus laevis Embryos
Characterization of Mosaicism in Various Easy-to-Acquire Body Tissues Such as Buccal Smears, Hair Ro
Cell Immobilization with Phosphorylated Polyvinyl Alcohol (PVA) Gel
A Rat Middle Cerebral Artery Occlusion Model and Intravenous Cellular Delivery
Live Cell Calcium Imaging of Dissociated Vomeronasal Neurons
Preparation of RNA Dot-Blots
Molecular Imaging and Tracking Stem Cells in Neurosciences
细胞房工作守则
PCR反应条件的选择
dictyBase and the Dicty Stock Center
Freezing and Thawing of Mammalian Cell Lines
Tips for the Functionalization of Nanoparticles with Antibodies
Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins
Neural Patterning and CNS Functions of Wnt in Zebrafish
Detection of Cells Programmed to Die in Mouse Embryos
Clinical Studies of Antisense Oligonucleotides for Cancer Therapy
Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation
Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells
Drosophila Cell Lines as Model Systems and as an Experimental Tool
Quantitation of Transient Gene Expression
Basics of Flow Cytometry
Quantitative Estimation of Tissue Blood Flow Rate
Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin
Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monola
Cloning of the 3 Noncoding Regions from Several Members of Heat Shock Protein Gene Families by Diffe
Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryo
Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients
Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development
Strategies to Insulate Lentiviral Vector-Expressed Transgenes
Detection of Telomerase Activity in Neural Cells
Assay for the Micrococcal Nuclease
Enrichment of Human Platelet Membranes for Proteomic Analysis
Long-Term Culture-Initiating Cell Assay for Mouse Cells
Cell Disruption and Subcellular Fractionation
Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells into Cardiomyocytes
viral immunity
Assessing Chronological Aging in Saccharomyces cerevisiae
Xenopus Cell-Free Extracts to Study DNA Damage Checkpoints
Prenylation Assays for Small GTPases
Fluorescent In Situ Hybridization of DNA Probes in the Interphase and Metaphase Stages of the Cell C
Live Imaging of Drosophila Myoblast Fusion
Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish
Chromosomal Changes and Cell Cycle Checkpoints in Mammalian Cells
Mesenchymal Stem Cells from Adult Bone Marrow
Evaluating the Therapeutic Potential of mTOR Inhibitors Using Mouse Genetics
How do I decontaminate my tissue culture (Invitrogen)
图示法展示细胞主要成分
微生物检验实验室墨汁染色标准操作规程
微生物实验室常见20种培养基配方大全！
常用细胞培养基 & 完全培养基配置方法
银耳栽培的培养基配方有哪几种？如何选择？
猪内皮素-1（ET-1）酶联免疫分析（ELISA）
Advanced Methodologies in Flow Cytometry
伴肌动蛋白(nebulin)
Synchronization of In Vitro Maturation in Porcine Oocytes
大鼠白细胞介素8（IL-8）酶联免疫分析
Apoptosis: Miniassay
Dye Ligand Chromatography
Quantitative PCR: A Survey of the Present Technology
Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers
Expression Cloning of Signaling Proteins Regulated by Cell Adhesion
Generation of Lentiviral Vectors for Use in Skeletal Muscle Research
Gene Profiling Studies in Skeletal Muscle by Quantitative Real-Time Polymerase Chain Reaction Assay
Histomorphometry in Rodents
水与平衡盐溶液
Derivation and Characterization of Alveolar Epithelial Cells From Murine Embryonic Stem Cells In Vit
Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Cartilage Ma
Comparative Genomic Hybridization on Single Cells
Real-Time PCR Expression Profiling of MMPs and TIMPs
Identification and Sequence Analysis of RNA-Protein Contact Sites by N-Terminal Sequencing and MALDI
Protein Kinase C: Measurement of Translocation, Activation, and Role in Cellular Responses
Lysosome Isolation in Isotonic Sucrose
Cell Migration into a Wounded Area In Vitro
Rationally Designed Ligands for Use in Affinity Chromatography: An Artificial Protein L
Immunoassay for Single-Stranded DNA in Apoptotic Cells
Neural Tube Defects
小鼠淋巴细胞脉络丛脑膜炎(LCM)酶联免疫分析（ELISA）
Isolation of Nucleoli from Ehrlich Ascites Tumor Cells and Dynamics of Nascent RNA within Isolated N
相变(phase transition)
Gene Transfer Techniques in Whole Embryo Cultured Post-implantation Mouse Embryos
Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy
XenopusEgg Extracts as a Model System for Analysis of Microtubule, Actin Filament, and Intermediate
Antibody Production and Immunocytochemical Localization of Amino Acid Transporters
Spatiotemporal Stimulation of Single Cells Using Flow Photolysis
Flow Cytometry of Fibroblast Nuclei for DNA content
In Vitro Assays for Axonal Growth and Targeting
Combinatorial Chemistry in Steroid Receptor Drug Discovery
Three-Dimensional Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells
Tubulin Basics
Production and Validation of Recombinant Adeno-Associated Virus for Channelrhodopsin Expression in N
Isolation of Circulating Angiogenic Cells
Purification of U Small Nuclear Ribonucleoprotein Particles
细胞化学染色分类技术
An Improved Rapid Method of Isolating RNA from Cultured Cells
Cell-Cell Interactions on Solid Matrices
Oligonucleotide-Directed Random Mutagenesis Using the Phosphorothioate Method
Implication of the ERK Pathway on the Post-transcriptional Regulation of VEGF mRNA Stability
Duplex Unwinding and RNP Remodeling With RNA Helicases
In Vitro Delivery of Drugs and Other Molecules to Cells
Histone Modification Profiling in Normal and Transformed Human Embryonic Stem Cells Using Micro Chro
Glutaraldehyde-Mediated Protein Immobilization
Reprogramming of Liver to Pancreas
Assays for Hypermethylation of the BRCA1 Gene Promoter in Tumor Cells to Predict Sensitivity to PARP
Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo
Preparation of tubulin
Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines
Detecting Polymorphisms in Human Longevity Studies: HLA Typing and SNP Genotyping by Amplicon Sequen
Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatograph
In Vivo Tumor Formation From Primate Embryonic Stem Cells
Embryonic Rodent Brain Cells in Culture
Isolation and Culture of Epithelial Stem Cells
Retroviral Cell Lineage Analysis in the Developing Chick Heart
Proteomic Analysis of Dictyostelium discoideum
Derivation of Epidermal Colony-Forming Progenitors From Embryonic Stem Cell Cultures
The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity
Antisense Inhibition: Oligonucleotides, Ribozymes, and siRNAs
细胞表面(cell surface)
Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embr
Activity Assays for Receptor-Interacting Protein Kinase 1:A Key Regulator of Necroptosis
Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Dro
Unbiased Stereological Method to Assess Proliferation Throughout the Subependymal Zone
In Situ End-Labeling of Fragmented DNA and the Localization of Apoptosis
Talin and Signaling Through Integrins
细胞同步化的方法总结
Review of Human DC Subtypes
Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation
常规组织传代培养
Immunochemical Methods for the Peptidomic Analysis of Tachykinin Peptides and Their Precursors
Analysis of Nuclear Export Using Photoactivatable GFP Fusion Proteins and Interspecies Heterokaryons
In Vivo Matrigel Migration and Angiogenesis Assays
Vesicular Phospholipid Gels
Localization of HIV-1 DNA and Tumor Necrosis Factor-α mRNA in Human Brain Using Polymerase Chain Rea
兔子髓过氧化物酶(MPO)酶联免疫分析（ELISA）
Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis
Ciona Genetics
Role of Caveolin-1 in the Regulation of Pulmonary Endothelial Permeability
Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Em
Using Molecular Tracers to Assess the Integrity of the Intestinal Epithelial Barrier In Vivo
血清使用过程中应注意的问题和血清质量的决定因素
小鼠染色体重组技术
鲍曼不动杆菌感染动物模型-甲氨蝶呤免疫抑制小鼠鲍曼不动杆菌感染肺炎动物模型
小鼠胚胎干细胞分化潜能检测-体内分化-畸胎瘤实验验证ES细胞多能性
小鼠胚胎干细胞分化潜能检测-体外分化-拟胚体方法检测ES多能性方法
Restriction Enzyme-Mediated Integration (REMI) Mutagenesis
Cell-Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein -Subunits in Madin-Da
Posttranscriptional Regulation of Ornithine Decarboxylase
嵌合体实验分析ES细胞分化潜能-四倍体补偿方法得到完全来源于ES的嵌合体动物
人白细胞介素-35（IL-35）酶联免疫分析（ELISA）
嵌合体实验分析ES细胞分化潜能-聚合法制作嵌合体小鼠
嵌合体实验分析ES细胞分化潜能-激光破膜系统辅助ES细胞显微注射法制作嵌合体小鼠
单体隔离蛋白(monomer-sequenstering protein)
TALEN 的组装构建-TALEN 多等级连接法
GAMMA IRRADIATION OF PMEF
TALEN 的组装构建-TALEN 单元组装法构建法
Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines
Tissue Culture of Skeletal Muscle
TALEN 的组装构建-磁珠载体 TALEN 组装构建法
Experimental Validation of MicroRNA Targets Using a Luciferase Reporter System
TALEN 的活性检测-限制性内切酶法检测 TALEN 活性
Diagnostic Challenges of Mitochondrial Disorders: Complexities of Two Genomes
TALEN 的活性检测-非配对内切酶法-T7E1 法检测 TALEN 活性
TALEN 的活性检测-luciferase SSA 法检测 TALEN 活性
Cellular Stress and Protein Misfolding During Aging
The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration
TALEN 敲除基因-TALEN 结合核移植技术进行基因敲除
TALEN 敲除基因-受精卵显微注射 TALEN mRNA 敲除基因
基于 CRISPR／Cas9 系统的动物基因敲除技术
iPS细胞制备技术-大鼠iPS细胞的制备
iPS细胞制备技术-小鼠iPS细胞的制备
基于 RNA 干扰的动物基因敲低技术
基于化学物质 ENU 的随机诱变技术
长骨干骨折动物模型-常用中型动物骨折模型
长骨干骨折动物模型-小鼠胫骨打孔模型
长骨干骨折动物模型-小鼠稳定骨折模型
长骨干骨折动物模型-小鼠不稳定骨折模型
大鼠ES细胞技术-大鼠ES细胞的体外培养
大鼠ES细胞技术-大鼠ES的建系
大鼠ES细胞技术-饲养层细胞（feeder）制备
大鼠ES细胞技术-胚胎成纤维细胞（MEFs）的制备
长骨干骨缺损动物模型
动物成体干细胞制备技术-神经干细胞的制备
动物成体干细胞制备技术-造血干细胞的制备
动物成体干细胞制备技术-脐带间充质干细胞的制备
动物成体干细胞制备技术-脂肪干细胞的制备
动物成体干细胞制备技术-密度梯度离心法制备骨髓间充质干细胞
动物成体干细胞制备技术-全骨髓贴壁筛选法制备骨髓间充质干细胞
感染性骨缺损动物模型
膝关节软骨缺损动物模型
胫骨干骺端截骨延长动物模型
股骨头缺血性坏死动物模型
遗传毒性试验-染色体畸变试验
遗传毒性试验-骨髓微核试验
类风湿关节炎动物模型
植入后局部反应试验-肌肉植入试验
植入后局部反应试验-皮下植入试验
植入后局部反应试验-骨植入试验
菌群移植模型实验技术-菌群检测
菌群移植模型实验技术-菌群移植模型动物的饲养管理
菌群移植模型实验技术-菌群移植模型的建立
裸鼠荷人成骨肉瘤模型
活体卵巢采集卵母细胞-活体输卵管采集卵母细胞
Analysis of G-Proteins Regulating Signal Transduction Pathways
细胞生存条件
弹性蛋白(elastin)
Manipulation of Hedgehog Signaling in Xenopus by Means of Embryo Microinjection and Application of C
Recovery, Isolation, Identification, and Preparation of Spermatozoa from Human Testis
Derivation of Contractile Smooth Muscle Cells from Embryonic Stem Cells
Separation of Dna by Capillary Electrophoresis
Serum-Free Media for Neural Cell Cultures: Adult and Embryonic
Differentiation and Dynamic Analysis of Primitive Vessels from Embryonic Stem Cells
Single Muscle-Fiber Isolation and Culture for Cellular, Molecular, Pharmacological, and Evolutionary
Combinatorial Stem Cell Mobilization in Animal Models
Noninvasive Metabolic Assessment of Single Cells
High Performance Liquid Chromatography/Electron Spin Resonance/Mass Spectrometry Analyses of Lipid-D
Protection of Purified Human Hematopoietic Progenitor Cells by Interleukin-1 or Tumor Necrosis Facto
Identification of Oocyte Progenitor Cells in the Zebrafish Ovary
Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction
Laser Microdissection Microscopy: Application to Cell Culture
Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes
Liposomal Reconstitution of Monotopic Integral Membrane Proteins
Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors
Mannosylated Liposomes for Targeted Vaccines Delivery
Endocrinology and Cell Signaling in Human Villous Trophoblast
Laser Ablation of the Microtubule Cytoskeleton: Setting Up and Working with an Ablation System
Identification of Protein Interactions by Yeast Two-Hybrid Screening and Coimmunoprecipitation
Tubulin Preparat
Assay of Matrix Metalloproteinases Against Matrix Substrates
人肝细胞生长因子激活物（HGFA）酶联免疫分析（ELISA）
Neurospora crassa as a Model Organism for Mitochondrial Biogenesis
Electrophysiological Studies of Ion Channels and Therapeutic Potential With Adenovirus-Mediated Gene
PREPARATION OF MICROINJECTION PIPETTES
Application of In Vivo EPR for Tissue pO2 and Redox Measurements
Video-Enhanced Microscopy for Analysis of Cytoskeleton Structure and Function
Isolation and Short-Term Culture of Mouse Spermatocytes for Analysis of Meiosis
Imaging and Tracking of Bone Marrow-Derived Immune and Stem Cells
A Mouse Model of Diet-Induced Obesity and Insulin Resistance
Bacterial Expression, Purification, and Crystallization of Tyrosine Phosphorylated STAT Proteins
Expanding the Horizons for Single-Cell Applications on Lab-on-a-Chip Devices
Isolation of Total RNA From Neurospora Mycelium
Ultraviolet Irradiation of Cells
Electroporation of Cardiac Cells
Isodielectric Separation and Analysis of Cells
Future and Applications of Cloning
Identification and Analysis of Bioactive Peptides in Amphibian Skin Secretions
Human Nasal and Tracheo-Bronchial Respiratory Epithelial Cell Culture
Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Age
Three-Dimensional In Vitro Culture Techniques for Mesenchymal Stem Cells
Brain Phosphoinositide Extraction, Fractionation, and Analysis by MALDI-TOF MS
Forward RNAi Screens in Human Stem Cells
A Yeast Spliceosome Assay
Determination of Protein Turnover Rates in the JAK/STAT Pathway Using a Radioactive Pulse-Chase Appr
Eukaryote Growth Dynamics
Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantita
Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells Using
Cardiac Stem Cell Senescence
Human and Mouse Hematopoietic Colony-Forming Cell Assays
粗肌丝(think filament)
Metabolic Labeling and Click Chemistry Detection of Glycoprotein Markers of Mesenchymal Stem Cell Di
In Vivo Transplantation of Engineered Human Skin
Competitor Calibration and Analysis of Competitive Amplified PCR Products by High-Performance Liquid
Competitor Calibration and Analysis of Competitive Amplified PCR Products by High-Performance Liquid
Defining In Vivo Dendritic Cell Functions Using CD11c-DTR Transgenic Mice
Identifying and Searching for Conserved RNA Localisation Signals
In Vitro Assays for Measuring TGF Growth Stimulation and Inhibition
Histological Analysis of Neurodegeneration in the Mouse Brain
Measurement of Green Fluorescent Protein Expression and DNA Content in Unfixed Cells
Visualization and Manipulation of the Platelet and Megakaryocyte Cytoskeleton
Targeted Magnetic Liposomes Loaded with Doxorubicin
Generation of Mouse Bone Marrow-Derived Macrophages
Molecular Cloning, Tissue Distribution, and Chromosomal Localization of the Human Homolog of the R2/
Use of Kinase Inhibitors to Dissect Signaling Pathways
Tracking the Glycerophospholipid Distribution of Docosahexaenoic Acid by Shotgun Lipidomics
Studying S-Phase DNA Damage Checkpoints Using the Fission Yeast Schizosaccharomyces pombe
Delivery of Genes In Vivo Using Pulsed Electric Fields
Primary Kidney Proximal Tubule Cells
Hammerhead Ribozyme-Mediated Knockdown of mRNA for Fibrotic Growth Factors: Transforming Growth Fact
RNAi Screening in Cultured Drosophila Cells
Isolation of Undifferentiated and Early Differentiating Type A Spermatogonia from Pou5f1-GFP Reporte
Lentiviral Gene Transfer Method to Study Integrin Function in T Lymphocytes
Methods for Meiotic Chromosome Preparation, Immunofluorescence, and Fluorescence in situ Hybridizati
Structural Studies of Proteins in Solution Using Proton Nuclear Magnetic Resonance
MALDI-TOF Mass Spectrometry Approaches to the Characterisation of Insect Neuropeptides
Acetylation of Endogenous STAT Proteins
CSF Extract Prep for Spindle Assembly
受体酪氨酸激酶(receptor tyrosine kinase, RTKs)
细胞器DNA的荧光显微观察
人可溶性细胞间粘附分子3(sICAM-3)酶联免疫分析（ELISA）
T7 RNA Polymerase-Mediated Incorporation of 8-N3AMP Into RNA for Studying Protein-RNA Interactions
细胞的原代和传代培养
LC3 and Autophagy
T Cell Culture for Gammaretroviral Transfer
外排作用
研究T细胞产生的细胞因子的方法
自噬性溶酶体(autolysosome)
蛋白质（protein）
Passaging cells
细胞培养常见问题
常用细胞凋亡检测方法（二）
Stock solutions for tissue culture
HER信号通路图（11张）
心肌细胞流式检测AV/PI个人经验
活体卵巢采集卵母细胞-B型超声波法采集卵母细胞
活体卵巢采集卵母细胞-腹腔镜法采集卵母细胞
脑血管疾病动物模型-蛛网膜下腔出血动物模型
脑血管疾病动物模型-脑出血模型
脑血管疾病动物模型-脑缺血模型
全身毒性试验-重复接触全身毒性试验
全身毒性试验-热原试验
全身毒性试验-全身急性毒性试验
骨质疏松动物模型
坐骨神经缺损动物模型
屠宰后卵巢上采集卵母细胞-剥离法采集卵母细胞
屠宰后卵巢上采集卵母细胞-切割法采集卵母细胞
屠宰后卵巢上采集卵母细胞-抽吸法采集卵母细胞
动物实验外科手术切除术-睾丸摘除术
动物实验外科手术切除术-卵巢子宫切除术
动物实验外科手术切除术-卵巢摘除术
动物实验外科手术切除术-垂体切除术
动物实验外科手术切除术-脾切除术
动物实验外科手术切除术-肝切除术
动物实验外科手术切除术-胃造瘘及胃切除术
皮肤致敏试验-封闭贴敷试验
皮肤致敏试验-豚鼠最大剂量法
刺激试验-阴道黏膜刺激试验
刺激试验-直肠刺激试验
刺激试验-阴茎刺激试验
刺激试验-眼刺激试验
刺激试验-口腔黏膜刺激试验
刺激试验-皮内反应试验
刺激试验-动物皮肤刺激试验
阿尔茨海默病其他动物模型-大鼠侧脑室注入β-淀粉样蛋白（Aβ）模型
阿尔茨海默病其他动物模型-大鼠侧脑室注入选择性胆碱能毒剂模型
骨关节炎动物模型
帕金森病动物模型-基因转染动物模型
帕金森病动物模型-灵长类动物 Parkinson 病模型
帕金森病动物模型-旋转大鼠模型
口腔材料生物学评价动物实验-显性致死试验
口腔材料生物学评价动物实验-吸入毒性试验
口腔材料生物学评价动物实验-短期全身毒性试验经口途径
口腔材料生物学评价动物实验-盖髓试验
口腔材料生物学评价动物实验-牙髓牙本质试验
口腔材料生物学评价动物实验-根管内应用试验
骨髓损伤动物模型-化学损伤模型
骨髓损伤动物模型-横断损伤模型
骨髓损伤动物模型-缺血再灌注损伤模型
骨髓损伤动物模型-压迫损伤模型
骨髓损伤动物模型-可调控的挫伤模型
骨髓损伤动物模型-脊髓挫伤模型
癫痫动物模型-大鼠癫痫持续状态及自发发作模型
癫痫动物模型-大鼠失神动物模型
癫痫动物模型-大鼠最大电休克发作模型
常染色质(euchromatin)
膜蛋白放射性标记法(radioactive labeling procedure)
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
人可溶性细胞间粘附因子(sICAM)酶联免疫分析（ELISA）
显微操作术(micromanipulation)
减数分裂、有丝分裂永久制片的观察
整联蛋白(integrin)
5种MEM细胞培养基成分
内皮细胞培养步骤
Caspase信号级联通路图
Planar Patch Clamp: Advances in Electrophysiology
披网格蛋白小泡(clathrin-coated vesicle)
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
常用细胞凋亡检测方法
人细胞色素C（CytC）酶联免疫分析（ELISA）
胰岛素受体信号通路（5张）
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fi
胎牛血清产品等级介绍
大鼠血糖酶联免疫分析（ELISA）
人碱性成纤维细胞生长因子受体（bFGFR）酶联免疫分析(ELISA)
整合素信号通路图（5张）
原核细胞与真核细胞的区别点
Cryopreservation of Hybridomas
Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation
Transgene-Free Production of Pluripotent Stem Cells Using piggyBac Transposons
关于PI（碘化丙啶）的讨论
细胞增殖检测新技术——EdU 取代BrdU
细胞自噬的研究方法
细胞凋亡的研究进展
过氧化物酶标记测定法检测细胞凋亡
细胞活力测定技术
组织Caspase-3活性测定操作规程
TUNEL细胞凋亡检测程序
细胞分裂指数测定
细胞技术及活力测定
常用细胞凋亡检测方法（图）
细胞凋亡检测及相关分子检测
MTT（四唑盐比色实验）大汇总
细胞凋亡实验步骤及注意事项
Hoechst-PI染色法检测细胞凋亡
SH2B对生长、代谢和寿命调控的功能进化
区分细胞周期中的细胞与凋亡中的细胞
Stewart Laboratory Standard Operating Procedure
ABER在位活细胞分析的运用
凋亡荧光素检测系统常见问题
细胞生长检测8：三磷酸腺苷检测法（ATP法）
细胞周期的测定
线粒体膜势能的检测
关于细胞凋亡的讨论
如何做好细胞爬片
TUNEL法检测细胞凋亡实验原理和方法
DNA片断化检测
细胞凋亡的特点
细胞凋亡：像秋天落叶一样凋落的死亡方式
细胞凋亡的形态学观察
细胞凋亡检测技术与方法
细胞凋亡检测技术与方法
细胞凋亡的简介和检测
细胞凋亡
Programmed Cell Death In Situ Detection Using Terminal deoxynucleotidyl Transfer(TdT)
细胞生长检测5：NAG检测法
流式细胞仪的使用程序
凋亡细胞对胰蛋白酶和核糖核酸酶敏感性分析法
凋亡和细胞周期调控相关基因的应用
细胞凋亡检测：形态学特征的检测方法
培养细胞活力测定
细胞培养板的选择和使用
流式细胞仪
流式细胞仪技术(FCM)
DNA LADDER检测细胞凋亡的讨论
T细胞数量由细胞凋亡过程控制
细胞爬片经验
Flow Cytometry Assay to Measure Internalization of GPCRs
流式检测凋亡的讨论
细胞凋亡的形态学检测
肿瘤多药耐药与细胞凋亡逃逸
癫痫动物模型-大鼠复杂部分性癫痫模型
癫痫动物模型-大鼠部分简单性癫痫模型
椎间盘退变动物模型-结构模型
椎间盘退变动物模型-脊柱运动阶段不稳致椎间盘退变模型
椎间盘退变动物模型-双后肢大鼠动物模型
脑外伤动物模型-可控皮质撞击模型
脑外伤动物模型-液压冲击伤模型
脑外伤动物模型-自由落体冲击模型
乳头瘤病毒感染动物模型-转基因动物模型
乳头瘤病毒感染动物模型-移植瘤动物模型－轮状病毒感染动物模型
脊髓损伤动物模型-静力性压迫慢性损伤模型
脊髓损伤动物模型-钳夹模型
脊髓损伤动物模型-撞击损伤模型
脊髓损伤动物模型-脊髓横断模型
轮状病毒感染动物模型
单器官移植术-睾丸移植术（组织移植）
单器官移植术-肺移植术
单器官移植术-脾脏移植术
单器官移植术-心脏移植术
单器官移植术-肝脏移植术
单器官移植术-肾脏移植术
单器官移植术-小肠移植术
寄生虫病动物模型-包虫病动物模型
寄生虫病动物模型-日本血吸虫病动物模型
寄生虫病动物模型-阿米巴动物模型
寄生虫病动物模型-布鲁属丝虫动物模型
寄生虫病动物模型-疟原虫动物模型
寄生虫病动物模型-隐孢子虫动物模型
寄生虫病动物模型-肺孢子虫动物模型
寄生虫病动物模型
联合移植及多器官联合移植-猪腹部多器官移植
联合移植及多器官联合移植-大鼠原位肝肾联合移植
联合移植及多器官联合移植-大鼠异位心肺联合移植
联合移植及多器官联合移植-胰腺十二指肠联合移植
联合移植及多器官联合移植-胰肾联合移植
自发性前列腺增生动物模型
良性前列腺增生动物模型-雌雄激素联用法
细菌性前列腺炎动物模型-经尿道灌注大肠埃希菌法
细菌性前列腺炎动物模型-前列腺内注射大肠埃希菌法
非细菌性前列腺炎动物模型-乳酸盆底注射法制备非细菌性前列腺炎动物模型
非细菌性前列腺炎动物模型-免疫法制备非细菌性前列腺炎动物模型
非细菌性前列腺炎动物模型-化学法制备非细菌性前列腺炎动物模型
诱发性皮肤疾病动物模型-天疱疮动物模型
诱发性皮肤疾病动物模型-毒性接触性皮炎动物模型
诱发性皮肤疾病动物模型-变应性接触性皮炎动物模型
诱发性皮肤疾病动物模型-麻风动物模型-树鼩模型
诱发性皮肤疾病动物模型-麻风动物模型-裸鼠模型
诱发性皮肤疾病动物模型-麻风动物模型-犰狳模型
诱发性皮肤疾病动物模型-麻风动物模型-新生期摘除胸腺大鼠模型
诱发性皮肤疾病动物模型-麻风动物模型-免疫抑制小鼠模型
胚胎干细胞：胚胎发育早期囊胚中未分化的细胞
单链抗体可变区基因片段五聚体作用于凋亡诱导配体受体-2来增强其对癌症的治疗作用
凋亡形态学图片
胃癌细胞培养心得及真菌感染应对方案
癌细胞特征：不死性、迁移性和失去接触抑制
五大细胞信号通路
五大细胞信号通路研究进展
细胞培养中的支原体污染的预防及处理
关于MTT实验总结
MTT原理、步骤、结果分析方法及注意事项
细胞凋亡与坏死及细胞程序性死亡的区别
细胞凋亡的研究方法
Hoechst染色的讨论
Caspase-3活性的检测
为什么细胞凋亡会有特异性？
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
细胞爬片的保存和抗原修复问题总结
TNF的生物活性测定
细胞生长检测1：直接计数细胞
染色体制片程序
细胞生长检测2：MTT检测法
细胞凋亡检测-形态学
细胞增殖的检验方法：酸性磷酸酶法
FIXATION and DNA Staining for Cell Cycle Analysis
通过酵母老化细胞的副产物定量酵母时序寿命
测定芽殖酵母复制寿命
HMGB1对肝星状细胞细胞外基质合成的影响
Advanced Methodologies in Flow Cytometry
细胞凋亡试验常用的方法（MTT法、荧光法、DNA琼脂糖凝胶电泳法与流式细胞仪检测法）
细胞培养板的选择和使用（二）
MTT实验的一些细节问题
细胞培养板的选择和使用（四）
罗氏公司TUNEL细胞凋亡检测程序
细胞的分裂指数测定
Flow Cytometric Analysis Cell Cycle
The MTT Cell Proliferation Assay
DNA Fragmentation Assays for Apoptosis Protocol
细胞信号通路相关文献
细胞生长检测6：AlamarBlueTM摄入法
流式细胞术发展简史
第43期人类iPS技术短期培训班开始报名！
寻找组织工程的理想种子细胞
iPS细胞建立的多种选择
逆转录病毒载体介导DNA转染
iPS细胞面临的问题
慢病毒载体介导红色荧光蛋白基因转染人脑胶质瘤干细胞
核基质结合区修饰的附着体载体介导的EGFP基因体外表达
iPS细胞的应用
iPS细胞的诞生
重组蛋白诱导iPS细胞
小鼠表皮细胞与真皮细胞的原代获取
挽救细胞污染效果评价：巨噬细胞法效果颇尴尬
小鼠脾脏树突细胞的分离－塑料黏附和EA玫瑰花结富集DC
AUTOMACS系统自动分选体系分选小鼠CD4+ CD25+抑制性细胞
淋巴细胞的分离和激化实验
从小鼠中分离内皮细胞
小鼠脾脏树突细胞的分离－胶原酶消化制备脾脏细胞悬液
细胞分离技术
Isolate or Deplete Human CD4+ T Cells - Whole Blood, Buffy Coat, MNC
培养细胞的保存和复苏
HT1080细胞的传代经验
实验室菜鸟的细胞培养心得
DC培养的几点疑问
骨髓间充质干细胞(MSC)原代培养与传代培养
转管及转瓶培养
干粉培养基的配制
细胞培养基本概念和环境
正常大鼠主动脉内皮细胞的培养
肿瘤细胞/细胞系的培养
肿瘤细胞的原代培养方法
植物细胞培养技术
支原体污染
细胞培养中支原体污染的特点及检验
有关细胞支原体污染的经验讨论
细胞支原体污染的PCR检测
Preparation of low density, collagenase-digested splenocytes Protocol
新购进细胞株的问题
正常小鼠原代脑星形胶质细胞培养
正常小鼠原代骨骼肌细胞培养
诱发性皮肤疾病动物模型-麻风动物模型-免疫正常小鼠感染模型
白细胞减少症动物模型-60Co-γ射线建立小鼠白细胞减少症模型
白细胞减少症动物模型-食物中毒性白细胞缺乏症猫模型
白细胞减少症动物模型-环磷酰胺诱发的白细胞减少症动物模型
白细胞增多症动物模型-白细胞增多症兔模型
白细胞增多症动物模型-白细胞增多症犬模型
急性非淋巴（髓）细胞白血病动物模型-慢性粒细胞白血病小鼠模型
急性非淋巴（髓）细胞白血病动物模型-粒单核细胞白血病（WEHI-3）小鼠模型
遗传工程、自发性皮肤疾病动物模型-异种移植银屑病动物模型
遗传工程、自发性皮肤疾病动物模型-自发性小鼠 SLE 的系统性红斑狼疮动物模型
急性弥散性血管内凝血动物模型-内毒素构建急性弥散性血管内凝血动物模型
缺铁性贫血动物模型-低铁饲料加多次少量放血法构建缺铁性贫血动物模型
特发性血小板减少性紫瘢动物模型-特发性血小板减少性紫瘢小鼠模型
特发性血小板减少性紫瘢动物模型-特发性血小板减少性紫瘢兔模型
血栓性疾病动物模型-多发性脑血栓动物模型
血栓性疾病动物模型-急性脑血栓动物模型
血栓性疾病动物模型-急性肺血栓栓塞症动物模型
血栓性疾病动物模型-电刺激血栓形成动物模型
血栓性疾病动物模型-动－静脉旁路血栓形成动物模型
再生障碍性贫血动物模型-γ射线照射构建再障模型
再生障碍性贫血动物模型-苯致骨髓抑制构建再障模型
再生障碍性贫血动物模型-白消安致骨髓抑制构建再障模型
甲状腺功能减退动物模型-先天性甲减模型
甲状腺功能减退动物模型-抗甲状腺药物诱导甲减模型
药品生物制品成品检定常用动物实验-乳鼠试验
甲状腺肿动物模型-高碘性甲状腺肿动物模型
甲状腺肿动物模型-低碘性甲状腺肿动物模型
抗毒素效价测定法检定药品生物制品成品实验-气性坏疽抗毒素效价测定法（小鼠试验法）
抗毒素效价测定法检定药品生物制品成品实验-白喉抗毒素效价测定法（家兔皮肤试验法）
自身免疫性甲状腺炎动物模型-Tg 致敏树突状细胞诱导自身免疫性甲状腺炎
自身免疫性甲状腺炎动物模型-完全佐剂联合应用诱导自身免疫性甲状腺炎
Graves 病动物模型-转基因致 Graves 病动物模型
Graves 病动物模型-表达 TSHR 的细胞免疫致 Graves 病动物模型
Graves 病动物模型-核酸免疫法致 Graves 病动物模型
疫苗效价测定法检定药品生物制品成品实验-吸附自喉疫苗效价测定法
疫苗效价测定法检定药品生物制品成品实验-吸附破伤风疫苗效价测定法
其它药物检查法检定药品生物制品成品实验-葡萄糖酸锑钠毒力检查法
激素生物测定法检定药品生物制品成品实验-卵泡刺激素生物检定法
激素生物测定法检定药品生物制品成品实验-精蛋白锌胰岛素注射液延缓作用检查法
激素生物测定法检定药品生物制品成品实验-升压物质检查法
激素生物测定法检定药品生物制品成品实验-升压素生物测定法
激素生物测定法检定药品生物制品成品实验-生长激素生物测定法（大鼠体重法）
过敏反应检查法检定药品生物制品成品实验
心阳虚证动物模型-高脂饮食加脑垂体后叶素法建立心阳虚证动物模型
心阳虚证动物模型-普罗帕酮法建立心阳虚证动物模型
心血瘀阻证动物模型-犬冠状动脉结扎法建立心血瘀阻证动物模型
肝郁证动物模型-电刺激加噪声干扰法建立肝郁证动物模型
肝郁证动物模型-慢性应激程序法建立肝郁证动物模型
肝郁证动物模型-限动水浸郁怒法建立肝郁证动物模型
肝郁证动物模型-枷锁模具激怒法建立肝郁证动物模型
IHC-让肿瘤原形毕露【新手入门】
如何做好裸鼠皮下荷瘤【新手入门】
qPCR原理和方法都在这里【新手入门】
原代细胞培养和传代培养该用什么方法？
正常大鼠原代心肌细胞培养
细胞培养中的黑胶虫污染
小鼠脑微血管内皮细胞的体外培养
做细胞冻存的人都经历过的这些致命败笔！你逃过了吗？
细胞培养的一些问题
黑胶虫？你真的了解吗？
黑胶虫
三维细胞培养-无支架培养模式
三维细胞培养-支架培养模式
细胞的复苏经验总结
一种体外扩增人γδΤ细胞的新方法
FRTL－5细胞的传代
C2C12细胞的分化心得
原代细胞计数技术
原代细胞复苏技术
原代细胞复苏的基本操作方法
原代细胞分离和培养基本步骤
原代细胞冻存技术
原代微血管内皮细胞的体外分离培养
Tissue Culture - Storage of Cell Lines
小鼠成骨细胞的培养和传代
A general protocol for staining cell for cytometry analysis
如何把细胞养的更漂亮？
细胞株采购注意事项
菌体/细胞裂解的常用方法和配方汇总
细胞体外暴露方法的一个重要问题及 CULTEX 的优化方案
在进口优质血清缺乏情况下如何选择细胞培养
细胞被污染了，你发现了么？
细胞培养用血清中的纳米细菌和黑胶虫有关系么？
多聚赖氨酸（Poly-L-Lysine）的配制、保存、使用总结
促进干细胞生长并抑制分化新培养基
胰酶使用注意及贴壁细胞的消化
大鼠胰岛细胞原代培养
制备人干细胞无动物血清培养时的有效添加剂—混合人血小板裂解物
动物气管和支气管假复层纤毛柱状上皮细胞的培养
肿瘤细胞体外传代培养及保种
人视网膜色素上皮细胞的传代
原代细胞培养技术
细胞培养技术中的关键因素－细胞操作功夫在手！
失败—成功—体味—收获—思考—交流，讲述你我细胞培养实验中的故事
兔膀胱平滑肌细胞的分离、培养和鉴定
细胞冻存和复苏技术
耗材对细胞培养的影响
细胞传代培养
Cell Suspension Culture of Arabidopsis
原代细胞的培养和维持
细胞单克隆的分离方法
淋巴细胞培养注意事项
血清使用问题的总结
细胞培养技术扫盲——入门必知篇
细胞冻存、解冻方法与细胞计数
大鼠间充质干细胞培养
胚胎干细胞培养标准化操作规程(SOP)
细胞培养中污染的清除和预防
原代细胞传代技术
细胞培养常见问题汇总
体外细胞的原代培养、传代培养、冻存和复苏
牙髄细胞的传代
原代细胞培养实验室组建的基本要求
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
分离肺泡上皮细胞的步骤
原代细胞的取材
培养基手册
大鼠原代足细胞的培养（图）
细胞培养常见问题解答
经验总结：关于细胞消化
细胞培养小技巧---操纵时间的艺术
禽马立克氏病病毒转化的T淋巴细胞系MSB1的传代
血清使用过程中应注意的问题
小鼠树突状细胞培养攻略
传代细胞的培养和维持
细胞培养中常见的污染情况总结
原代培养海马神经元经验
乳鼠心肌细胞培养方法详述
LAK/TIL细胞的制备
细胞培养室的设置、设备和准备工作
细胞培养基大全
神经干细胞的培养
大鼠皮层神经元原代培养
细胞培养的三不要
细胞污染原因
如何把细胞养的更漂亮？
知名专家谈细胞培养耗材表面选择
细胞培养关键技术：培养基选择及成分确定
流式结果怎么看
SDS-PAGE胶配制及注意事项
浓缩胶缓冲液全解析：定义、用途与制备
蛋白电泳分离胶和浓缩胶浓度计算方法详解
离子交换色谱洗脱缓冲液浓度优化
肝郁证动物模型-夹尾激怒法建立肝郁证动物模型
肝血瘀阻证动物模型-鸭乙型肝炎病毒 (DHBV) 诱发肝血瘀阻证动物模型
肝阴虚证动物模型-四氯化碳加温热中药法复制肝阴虚证动物模型
肝阴虚证动物模型-四氯化碳加甲状腺素法复制肝阴虚证动物模型
肝火上炎证动物模型-急性虹膜睫状体炎模型替代法复制肝火上炎证动物模型
肝阳上亢证动物模型-肾动脉狭窄加灌附子法复制肝阳上亢证动物模型
肝癌动物模型建模实验-VX2 移植性肝癌模型法
肝癌动物模型建模实验-二乙基亚硝胺诱导法
肝硬化动物模型建模实验
肝移植动物模型建模实验-二袖套法大鼠原位肝移植模型
急性胰腺炎动物模型建模实验-L-精氨酸腹腔注射法制备胰腺炎模型
胰腺癌动物模型建模实验-裸鼠移植瘤模型
胰腺癌动物模型建模实验-化学因素诱导动物模型
胃癌动物模型建模实验-裸鼠人胃癌原位移植模型
胃癌动物模型建模实验-大鼠诱发胃癌模型
结直肠癌动物模型建模实验-结直肠癌肝转移动物模型
结直肠癌动物模型建模实验
急性梗阻化脓性胆管炎动物模型建模实验
膜迷路积水动物模型建模实验
耳鸣动物模型建模实验
支气管哮喘动物模型建模实验-卵白蛋白诱发法构建支气管哮喘动物模型（支气管哮喘动物模型建模实验）
动脉粥样硬化动物模型-免疫法
系膜增生性肾炎动物模型
药物致心肌坏死模型-垂体后叶素法
药物致心肌坏死模型-儿茶酚胺法
微小病变性肾病动物模型-嘌呤霉素肾病动物模型
低灌注型急性肾衰竭动物模型
诱发性高血压动物模型-高果糖饮水诱导高血压模型
诱发性高血压动物模型-高盐饮食诱发高血压动物模型
诱发性高血压动物模型-手术诱发性高血压动物模型
诱发性高血压动物模型-药物诱发性高血压动物模型
诱发性高血压动物模型-应激性高血压动物模型
TCR人源化动物模型
人源化乙肝感染小鼠模型-人源化Fah-/-Rag2-/-II2rg-/-小鼠模型
心肌缺血引起的心力衰竭动物模型-冠状动脉结扎法
心肌缺血引起的心力衰竭动物模型-冠状动脉微栓塞法
人源化抗体转基因小鼠模型
机械损伤诱导血栓形成模型-结扎法
机械损伤诱导血栓形成模型-电损伤法
诱发性糖尿病动物模型-病毒诱导糖尿病模型
诱发性糖尿病动物模型-手术及药物联合制作糖尿病模型
诱发性糖尿病动物模型-四氧嘧啶（alloxan）诱导糖尿病模型
诱发性糖尿病动物模型-链脲佐菌素（STZ）诱导糖尿病模型
诱发性糖尿病动物模型-结扎胰管加高糖饮食诱导糖尿病模型
诱发性糖尿病动物模型-胰腺切除方法诱导糖尿病模型
肝微粒体制备
自发性糖尿病动物模型-非人灵长类自发性动物模型
自发性糖尿病动物模型-猪自发性糖尿病模型
自发性糖尿病动物模型-猫科动物自发性糖尿病模型
自发性糖尿病动物模型-啮齿类自发性糖尿病动物模型
VivaCell品牌介绍
GraphPad Prism 9.0安装破解激活教程
生命科学细分四大领域，龙头企业盘点！
PDQuest 分析软件安装教程
MestReNova14.0中文版安装教程
生物学常用软件
转基因技术糖尿病动物模型-线粒体糖尿病模型
转基因技术糖尿病动物模型-2型糖尿病模型
妊娠糖尿病模型-四氧嘧啶法制作妊娠糖尿病模型
妊娠糖尿病模型-STZ法制作妊娠糖尿病模型
慢性马兜铃酸肾病动物模型
高尿酸血症及尿酸性肾病动物模型
子宫内膜异位症动物模型-异体移植模型
子宫内膜异位症动物模型-自体移植模型
肝脏灌流实验
药物体内代谢实验
输卵管炎 (盆腔炎)动物模型-化学性输卵管炎动物模型（苯酚胶浆法）
输卵管炎 (盆腔炎)动物模型-衣原体致输卵管炎模型
输卵管炎 (盆腔炎)动物模型-细菌致输卵管炎模型
狼疮样肾炎动物模型
肺气肿动物模型-吸烟诱导法制备肺气肿动物模型
肺气肿动物模型-酶诱导法制备肺气肿动物模型（肺气肿动物模型）
多囊卵巢综合征动物模型-非甾体类芳香化酶抑制剂（来曲吨）造模法
多囊卵巢综合征动物模型-胰岛索 (insulin, INS) 联合绒促性素造模法
多囊卵巢综合征动物模型-孕激素联合绒促性素造模法
多囊卵巢综合征动物模型-雌激素造模法
多囊卵巢综合征动物模型-雄激素造模法
卵巢过度刺激综合征动物模型
庆大霉素肾病动物模型
卵巢早衰动物模型-自身免疫法
卵巢早衰动物模型-物理化学致损模型
烧伤动物模型-吸入性损伤动物模型-离体肺叶吸入性损伤模型
烧伤动物模型-吸入性损伤动物模型-烟雾吸入性损伤动物模型
烧伤动物模型-吸入性损伤动物模型-蒸汽吸入性损伤动物模型
烧伤动物模型-烧伤代谢营养研究的动物模型-贵州小型香猪胃造口、颈静脉以及门静脉和肠系膜静脉插管模型
烧伤动物模型-烧伤代谢营养研究的动物模型-空肠旷置-胃造口-烧伤家兔模型
烧伤动物模型-烧伤代谢营养研究的动物模型-胃肠性持续喂养豚鼠模型
烧伤动物模型-烧伤免疫实验动物模型
烧伤动物模型-烧伤感染动物模型
烧伤动物模型-烧伤休克动物模型
烧伤动物模型-磷烧伤动物模型的模型
烧伤动物模型-小鼠蒸汽烫伤模型
烧伤动物模型-动物皮肤热水烫伤模型
烧伤动物模型-凝固汽油致不同动物皮肤烧伤模型
烧伤动物模型-溴钨灯光辐射致犬皮肤烧伤
烧伤动物模型-溴钨灯光辐射致大白鼠皮肤烧伤实验
肝脏移植动物模型-猪肝移植
肝脏移植动物模型-大鼠肝移植
保留脾脏功能手术动物模型-大鼠脾脏部分或次全切除模型
保留脾脏功能手术动物模型-犬部分脾切除模型
复合伤动物模型-放射复合伤造血干细胞检测模型
复合伤动物模型-放射复合伤造血组织 DNA 含量检测模型
复合伤动物模型
复合伤动物模型-光辐射灯致伤模型
复合伤动物模型-放射损伤模型
肺水肿动物模型-肾上腺素注射法制备肺水肿动物模型
StyleWriter4英语论文润色神器安装使用教程
细胞自噬与肿瘤的关系
细胞自噬机制【小白必看】
SnapGene 6.0.2 中文版安装教程
细胞自噬大盘点
Pull-down实验大家族一览
GST pull-down技术原理及常见问题分析
缓冲液参考中心
生物实验常用缓冲液配方指南
Primer Premier 6下载安装使用激活教程
PyMOL 2.5.7安装教程
DNAClub DNA 处理软件
肺水肿动物模型-氯化铵中毒法制备肺水肿动物模型（肺水肿动物模型）
交通伤动物模型-颌面部撞击伤动物模型
交通伤动物模型-汽车碰撞防护栏致交通伤动物模型
交通伤动物模型-汽车正向碰撞交通伤动物模型
交通伤动物模型-钟摆式颅脑减速伤动物模型
交通伤动物模型-坠落式颅脑减速伤动物模型
交通伤动物模型-胸部撞击伤动物模型
交通伤动物模型-正碰交通伤动物模型
新型尿酸性肾病动物模型-
冲击伤动物模型-水下冲击致伤模型
冲击伤动物模型-冲击波负压致伤模型
冲击伤动物模型-点爆源爆炸冲击伤动物模型
冲击伤动物模型-生物激波管冲击伤动物模型
冲击伤动物模型-爆炸冲击伤动物模型
胎儿宫内生长迟缓动物模型-营养不良造模法
胎儿宫内生长迟缓动物模型-子宫动脉结扎法
胎儿宫内生长迟缓动物模型-被动吸烟法
高速投射物致伤动物模型-坐骨神经震荡伤致伤实验模型
高速投射物致伤动物模型-腹部投射物致伤实验模型
高速投射物致伤动物模型-胸部投射物致伤实验模型
高速投射物致伤动物模型-颅脑投射物致伤动物模型
高速投射物致伤动物模型-肢体软组织伤实验模型
IgA 肾炎动物模型-大肠埃希菌外膜蛋白 (OMPs) 诱发大鼠 IgA 肾炎模型
人或动物脑毛细血管原代内皮细胞的分离
血细胞的分离
有关血清的常见问题
IgA 肾炎动物模型-葡聚糖 G200(dextran G200) 诱发大鼠 IgA 肾炎模型
血清热灭活―您是否在浪费时间？
常见细胞系细胞培养基应用选择
细胞培养基应用选择
细胞培养基选择原则
细胞的传代培养
小鼠单个核细胞分离去除脾脏细胞悬液中的红细胞
细胞污染的一些总结和心得
各类细胞培养小结
IgA 肾炎动物模型-口服牛血清白蛋白 (BSA) 和葡萄球菌肠毒素 B(SEB) 复合法诱发大鼠 IgA 肾炎模型
香樟组培快繁技术研究进展
体外培养细胞成功率分析
细胞大规模培养技术之细胞滚瓶培养
细胞培养过程中有哪些污染
PriCells: 中国人组织源性原代细胞是最适于在中国进行体外检测和药物研发进行细胞培养的科研单位和生物公司
动物细胞的大规模培养技术
Good Cell Banking Practices
SP 2/0细胞的传代经验
正常兔骨髓间充质前体细胞的体外成软骨培养
肺炎动物模型-改良卡氏肺孢子虫肺炎动物模型
正常人表皮朗格汉斯细胞的培养
脐静脉内皮细胞原代培养
肺炎动物模型-麻疹病毒肺炎模型（肺炎动物模型）
The ECACC and its Collections
肺炎动物模型-铜绿假单胞菌肺炎模型
细胞培养基的定义
肺炎动物模型-肺炎支原体肺炎模型
正常大兔胆囊上皮细胞的培养
细胞培养的倍增和细胞培养的代数
Sedimentation Densities
肺炎动物模型-肺炎克雷伯菌肺炎模型（肺炎动物模型）
正常骨内成骨细胞原代培养
长白猪精原细胞的分离和纯化
Separation of Cells by Velocity Sedimentation
EFK（马胎肾细胞）的传代
H-Thymidine Uptake by Cultured Cells
细胞培养基发展趋势和品质
肺炎动物模型-肺炎链球菌肺炎动物模型
肿瘤细胞培养方法简介
牙周膜成纤维细胞PDLC的传代
自然流产动物模型-抗磷脂抗体 (antiphospholipid antibody, APA) 诱导的流产模型
组织块无血清条件培养小鼠的颅神经嵴细胞
自然流产动物模型-溴隐亭致大鼠流产模型
正常大兔主动脉内皮细胞的培养
Cryopreservation and Storage of Cell Lines
原代培养结肠平滑肌细胞
细胞培养的基本原理与技术
细胞支原体污染一般情况及预防措施
细胞冻存复苏基本材料方法和细胞运输
B16细胞的传代
Epstein-Barr Virus Transformation of Lymphoblasts
动物细胞培养实验室构建方案
动物细胞融合
细胞培养升级篇
细胞培养中无菌操作技术
细胞培养技术的注意事项
细胞培养中的污染
细胞培养基本条件
“走进动物细胞体外培养技术”----德国Memmert CO2培养箱
原代细胞中各种组织消化方法集合
细胞实验技术及基本知识（一）
细胞培养液讨论
自然流产动物模型-CBA/J 与 DBA/2 小鼠自然流产模型
抗 Thy 1 肾炎动物模型
原位免疫复合物肾炎动物模型-阳离子化牛血清白蛋白肾炎动物模型
原位免疫复合物肾炎动物模型-凝集素及其抗体诱导肾炎动物模型
原位免疫复合物肾炎动物模型-Heymann 肾炎动物模型
基因改变引起的NAFLD 动物模型-KKAy小鼠构建NAFLD 动物模型
基因改变引起的NAFLD 动物模型-db／db小鼠构建NAFLD 动物模型
基因改变引起的NAFLD 动物模型-ob／ob小鼠构建NAFLD 动物模型
基因改变引起的NAFLD 动物模型-Adiponectin-/-小鼠构建NAFLD 动物模型
基因改变引起的NAFLD 动物模型-CD36-/-小鼠构建NAFLD 动物模型
基因改变引起的NAFLD 动物模型-SREBP-1c转基因小鼠构建NAFLD 动物模型
矽肺动物模型的建立
饮食诱导／药物处理形成获得性NAFLD动物模型-CDAA饮食诱导的NAFLD动物模型
饮食诱导／药物处理形成获得性NAFLD动物模型-HFD合并MCD饮食诱导小鼠形成NAFLD模型
饮食诱导／药物处理形成获得性NAFLD动物模型-蛋氨酸胆碱缺乏饮食诱导的NAFLD模型
饮食诱导／药物处理形成获得性NAFLD动物模型-高脂饮食-药物联合诱导的NAFLD模型
饮食诱导／药物处理形成获得性NAFLD动物模型-高脂饮食诱导的NAFLD模型
早产动物模型-细胞因子诱导的早产模型
早产动物模型-腹腔内重复使用脂多糖诱导早产模型
早产动物模型-宫腔内直接感染诱寻的早产模型
常见胃肠外科手术动物模型-小肠缺血再灌注模型
常见胃肠外科手术动物模型-犬短肠综合征模型
常见胃肠外科手术动物模型-犬全胃切除模型
常见胃肠外科手术动物模型-犬保留幽门的胃大部分切除术模型
常见胃肠外科手术动物模型-犬高选择性迷走神经切断加胃窦黏膜切除术模型
常见胃肠外科手术动物模型-犬高选择性迷走神经切断术模型
糖尿病肾病动物模型
肺肉芽肿性血管炎动物模型
抗肾小球基底膜肾炎动物模型-加速型抗GBM肾炎动物模型
抗肾小球基底膜肾炎动物模型-Masugi肾炎动物模型
羊水栓塞动物模型
胎盘传播疾病动物模型（胎盘屏障模型）
妊娠期肝内胆汁淤积症动物模型-小鼠构建模型
妊娠期肝内胆汁淤积症动物模型-孕兔构建模型
妊娠期高血压疾病动物模型-寒冷刺激诱导法
妊娠期高血压疾病动物模型-血管内皮损伤法
妊娠期高血压疾病动物模型-子宫胎盘缺血诱导法
慢性肾衰竭动物模型
慢性阻塞性肺疾病动物模型建模实验
慢性支气管炎动物模型建模实验-烟熏法构建慢性支气管炎动物模型
急性呼吸窘迫综合征动物模型建模实验-内毒素注射法构建急性呼吸窘迫综合征动物模型
肺纤维化动物模型建模实验-放射线法构建肺纤维化动物模型
肺纤维化动物模型建模实验-博来霉素法构建肺纤维化动物模型
肺肉芽肿性血管炎动物模型建模实验
肺气肿动物模型建模实验-吸烟诱导法构建肺气肿动物模型
肺气肿动物模型建模实验-酶诱导法构建肺气肿动物模型（肺气肿动物模型建模实验）
肺结核动物模型建模实验
肺动脉高压动物模型建模实验-低压低氧构建肺动脉高压动物模型
中医脾系证候动物模型-脾不统血证动物模型
中医脾系证候动物模型-脾阳虚证动物模型
中医脾系证候动物模型-脾阴虚证动物模型
中医脾系证候动物模型-脾气虚证动物模型
Isolation of Primary Fibroblasts from Mouse Embryos
细胞培养基的使用方法（微孔滤膜过滤除菌）
细胞培养基础知识
细胞培养用途
细胞中出现颗粒的原因以及解决办法
细胞培养常见问题分析
支原体预防&去除试剂选购指南
动脉粥样硬化患者VSMC的提取
培养细胞的常规观察
MSC的传代
伤口愈合/细胞划痕实验新方法分享
CHO细胞的传代、培养方法
动物细胞培养：细胞冻存与复苏
细胞冻存和复苏
原代细胞的培养与建系(一)
IBRS-2细胞的传代
Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method
无血清培养基一些基本配方
什么是无血清培养基？
细胞培养基本技术
细胞培养基本技术概述
细胞培养中的若干细节问题
无菌操作基本技术
细胞培养污染的途径、危害及预防措施
细胞培养污染的思考
细胞培养的污染及控制
胃癌三种细胞株（MGC-803、BGC-823、SGC-7901）的培养
MCF-7细胞的传代经验
我培养A549细胞的经验
新手养细胞的心得
A549、ECV304、2BS三种细胞的传代
皮肤成纤维细胞和THP-1细胞的传代经验
几种乳腺癌细胞的培养
膀胱癌T24细胞传代
RAW264.7细胞株的传代
卵巢癌细胞的传代
LLC-PK1(猪的近端肾小管细胞系)消化和传代
原代培养的成骨细胞、骨髓基质细胞、cos7细胞的传代
细胞复苏失败的原因分析
给做细胞爬片的战友一点小窍门
细胞传代是否一定要等24小时呢？
癌细胞的分离培养和接种
有关细胞缺氧实验的相关问题
Alternative Cell Culture Systems
细胞的原代培养
细胞的传代次数和间隔时间
微载体培养技术的介绍
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
关于ADMET—正确选择原代肝细胞
正常大兔主动脉平滑肌细胞培养
牛或猪大血管原代内皮细胞的分离-灌注酶消化法
正常人脐静脉内皮细胞的培养
人绒毛膜癌细胞株JAR的传代
MCF-7的传代
Preparation of NeoR Murine Embryonic Fibroblasts
In vitro culture of embryonic lungs
复苏L929记录
贴壁细胞传代-消化过程(图示)
细胞传代方法
平滑肌细胞培养方法
正常晶状体上皮细胞原代培养
体外细胞培养之细胞培养基的基本要求
如何预防细胞培养的污染问题
个别细胞的培养
特异性抗体免疫磁珠分选、分离、纯化原代细胞
星型胶质细胞原代培养
利用Gene Pulser Mxcell电穿孔系统高效转染原代细胞
兔晶状体上皮细胞培养方法和体会
原代胚胎成纤维细胞培养
ORGANOTYPIC KIDNEY CULTURES
Preparation of Embryonic Extract
基底动脉平滑肌细胞原代培养
正常人角膜上皮细胞培养
正常动物肺肥大细胞的培养
正常大鼠肺泡上皮细胞的培养
正常动物胸腺上皮细胞的培养
正常大鼠皮肤肥大细胞的培养
正常成熟破骨细胞原代培养
正常小鼠前脂肪细胞的培养
正常人肺微血管内皮细胞培养
正常人支气管上皮细胞培养
正常小鼠肾间质成纤维细胞的培养
我培养BHK－21的经验
正常大鼠巨噬细胞的培养
淋巴细胞转化
95-D细胞的传代经验
神经胶质细胞培养
什么是传代细胞培养？
少突胶质细胞原代培养
PriCells: Isolation, culture, and purification of Human Pulmonary Arterial Smooth Muscle Cells
HUVEC分离培养
中医肺系证候动物模型-肺虚痰阻证模型
人胃腺癌细胞MNK-45体内传代及接种实体瘤
细胞培养的一般过程
中医肺系证候动物模型-肺气虚血瘀证动物模型
子宫内膜上皮传代
ibidi：因专业而值得拥有—流体剪切力系统
动物细胞培养方法
细胞实验技术及基本知识
中医肺系证候动物模型-肺阴阳两虚证动物模型
心肌细胞的分离及培养
细胞培养用液的配制与消毒
中医肺系证候动物模型-肺阳虚证动物模型
中医肺系证候动物模型-肺阴虚证动物模型
中医肺系证候动物模型-肺气虚证动物模型
中医肺系证候动物模型-肺热证动物模型
中医肺系证候动物模型-寒饮蕴肺证家兔动物模型
中医肺系证候动物模型-风寒犯肺证动物模型
中医肾系证候动物模型-肾阳虚证动物模型
中医肾系证候动物模型-肾阴虚证动物模型
中医肾系证候动物模型-肾气虚证动物模型
中医温病证候动物模型-D-氨基酸半乳糖注射法
中医温病证候动物模型-细菌注射法
中医温病证候动物模型-内毒素注射法
血虚证候动物模型-免疫介导法
血虚证候动物模型-放射线损伤法
血虚证候动物模型-药物损伤法
血虚证候动物模型-喂饲缺铁饲料法
血虚证候动物模型-综合放血法
血虚证候动物模型-失血性贫血法
甲状腺相关眼病动物模型-TSHR 质粒免疫建立 Graves 眼病的动物模型
甲状腺相关眼病动物模型-TSHR 活化的脾细胞免疫建立 Graves 眼病的动物模型
甲状腺相关眼病动物模型-TSHR 基因转染的腺病毒免疫建立 Graves 眼病的动物模型
甲状腺相关眼病动物模型
眼表及角膜病动物模型-蒸发过强型干眼模型-睑板腺功能障碍性干眼模型
DNASTAR序列分析软件
眼表及角膜病动物模型-泪液分泌不足型干眼动物模型-阿托品药物性干眼模型
眼表及角膜病动物模型-猪-猴异种角膜移植模型
眼表及角膜病动物模型-猪-大鼠异种角膜移植模型
实验中的分离胶与浓缩胶浓度解析
眼表及角膜病动物模型-角膜划痕法真菌性角膜炎动物模型
眼表及角膜病动物模型-基质层注射法真菌性角膜炎动物模型
为什么浓缩胶对蛋白质有浓缩作用?
眼表及角膜病动物模型-细菌性角膜炎动物模型
眼表及角膜病动物模型-HSK复发感染小鼠模型
眼表及角膜病动物模型-HSK 原发感染小鼠模型
眼表及角膜病动物模型-空气携带抗原法诱导变应性结膜炎动物模型
浓缩胶和分离胶的区别
眼表及角膜病动物模型-卵白蛋白诱导变应性结膜炎动物模型
眼表及角膜病动物模型
营养不良动物模型-猪营养不良模型建立
营养不良动物模型-鼠营养不良模型建立
营养不良动物模型
营养性肥胖动物模型
铅中毒动物模型-铅中毒小鼠模型的建立
铅中毒动物模型-铅中毒家兔模型的建立
铅中毒动物模型
缺锌动物模型-缺锌动物模型的建立
新生儿败血症动物模型-肺炎球菌败血症模型的制作
新生儿败血症动物模型-盲肠结扎穿孔复制新生儿败血症动物模型
新生儿败血症动物模型-皮下注射活菌复制新生儿败血症动物模型
新生儿败血症动物模型-静脉注射活菌复制新生儿败血症动物模型
心气虚血瘀证动物模型-冠状动脉结扎法建立心气虚血瘀证动物模型
primer premier6 直装版
SSR标记介绍
人教版全册｜七年级生物必考重点知识，暑期预习必备！
细胞培养常用器材与试剂
细胞培养常用试剂配置
MKN-28胃癌细胞株的消化传代
神经细胞培养基质总结
细胞培养基的基本知识
细胞培养基分类
如何选用培养基
培养基
细胞培养经验
293-T细胞培养的注意事项
血管内皮细胞原代培养
大鼠脑微血管内皮细胞的分离与原代培养
细胞培养用滤网的相关知识
八年级上册生物，必背知识点清单
Gemini 血清：最具性价比的原装进口血清
在微反应器中以芯片为基础的三维细胞培养
大鼠系膜细胞传代
Sourcing of Cell Lines
为什么有人提倡细胞培养液中不要加抗生素
细胞传代的一般方法
差速贴壁纯化心肌细胞的相关问题
你的细胞有做过 STR 鉴定吗？
巨噬细胞培养
分享几种细胞的培养方法
细胞原代培养
培养胶质瘤干细胞
人类胚胎干细胞的冷冻和解冻
新生大鼠大脑皮层星形胶质细胞的原代培养
细胞培养之无菌技术注意事项汇总
大鼠嗅球成鞘细胞分离培养方法
怎么样才能减少污染的机会
细胞培养全攻略
细胞培养培养基
单核细胞分离原理、方法及要点
从小鼠脾脏分离小鼠天然杀伤细胞
Hepes缓冲液配制
正常大鼠唾液腺上皮细胞的培养
原代细胞无血清培养技术
培养细胞形态及形态分析
心气虚小鼠心肌细胞模型-心肌细胞缺氧再给氧损伤法建立心气虚小鼠心肌细胞模型
心气虚证动物模型-高脂性免疫损伤加慢性放血法建立心气虚证动物模型
心气虚证动物模型-控食、力竭、大剂量普蔡洛尔和垂体后叶素法建立心气虚证动物模型
心气虚证动物模型-控食、力竭及大剂量普萘洛尔复合因素法建立心气虚证动物模型
心气虚证动物模型-睡眠剥夺加垂体后叶素注射法建立心气虚证动物模型
心气虚证动物模型-睡眠剥夺法建立心气虚证动物模型
心气虚证动物模型
白内障动物模型-先天性白内障动物模型-药物致畸法
白内障动物模型-先天性白内障动物模型-手术造模法
白内障动物模型-辐射性白内障动物模型
白内障动物模型-紫外线辐射性白内障动物模型
白内障动物模型-穿通伤白内障动物模型
白内障动物模型--钝挫伤白内障动物模型
白内障动物模型-半乳糖性白内障模型
白内障动物模型-亚硒酸盐诱导白内障模型
白内障动物模型
青光眼动物模型-自然青光眼动物模型
青光眼动物模型-烧灼巩膜上静脉诱发的青光眼动物模型
青光眼动物模型-血细胞诱导的青光眼动物模型
Low Speed Separaton of Cells
青光眼动物模型-甲基纤维素诱导的青光眼动物模型
建立细胞系或细胞株
青光眼动物模型-皮质类固醇诱导的青光眼动物模型
细胞传代吹打减少气泡的方法
细胞实验技术及基本知识（二）
人体干细胞成功造出人工牙齿
SK-BR-3（乳腺腺癌细胞系）的传代方法
肿瘤组织源性的原代细胞培养
细胞冻存与复苏技术（一）
关于细胞污染的一些总结和心得
青光眼动物模型-高渗盐水诱导的青光眼动物模型
合成细胞培养基
青光眼动物模型-激光诱导的青光眼动物模型
培养细胞的冷冻保存与复苏
葡萄膜炎动物模型-过继转移诱导葡萄膜炎动物模型法
细胞培养污染拯救及预防方法
葡萄膜炎动物模型-IRBP视网膜抗原诱导葡萄膜炎动物模型法
细胞冻存与复苏技术（二）
葡萄膜炎动物模型
如何优化您的免疫细胞培养？
用于胚胎干细胞中饲养层细胞的原代培养
视神经损伤动物模型-简易视神经损伤动物模型
神经细胞培养
视神经损伤动物模型-眼球钝挫伤冲击视神经损伤动物模型
人肺癌细胞（spc-A1、NCI-H446）传代
视神经损伤动物模型-眼眶钝挫伤冲击视神经损伤动物模型
动物细胞培养技术
大规模动物细胞培养的问题及对策
视神经损伤动物模型
细胞培养资料
细胞爬片中重要的细节问题
膀胱平滑肌细胞原代培养
细胞试验基本方法
大鼠心肌细胞分离
缺氧实验-组织中毒性缺氧法
缺氧实验-血液性缺氧法
缺氧实验-乏氧性缺氧法
急性炎症实验-大鼠炎症法
大鼠卵巢颗粒细胞的分离方法
急性炎症实验-大白鼠急性炎症法
PeproTech细胞因子和重组蛋白溶解必读
急性炎症实验-蟾蜍肠系膜炎症法
HepG2 细胞传代
SACC（腺样囊性癌细胞）的传代
水、电解质代谢紊乱相关实验-高钾血症动物模型
纯化心肌细胞差速贴壁的时间
水、电解质代谢紊乱相关实验-蟾蜍水肿模型
神经元原代培养
新一代纳米三维支架——让细胞重返仿真人体环境
水、电解质代谢紊乱相关实验-家兔实验性肺水肿模型
从单个核细胞中分离T细胞
16HBE14o－细胞株的传代
制取原代CEF的心得
细胞培养实验室
酸碱平衡紊乱-豚鼠酸碱平衡紊乱模型
酸碱平衡紊乱-兔酸碱平衡紊乱模型
心功能不全实验-急性右心衰竭法
心功能不全实验-实验性急性左心衰竭法
呼吸功能不全实验-家兔急性呼吸窘迫综合征法
呼吸功能不全实验-家兔急性人工气胸法
肝功能不全实验-家兔实验性肝细胞性黄疸法
肝功能不全实验-急性中毒性肝损伤法
肝功能不全实验-家兔肝性脑病法
弥散性血管内凝血-家兔急性 DIC 动物模型
实验性缺血-再灌注损伤实验-脑缺血再灌注实验（MCAO）
实验性缺血-再灌注损伤实验-家兔肠缺血-再灌注损伤法
实验性缺血-再灌注损伤实验-大鼠心脏缺血-再灌注损伤法
休克实验-狗感染性休克法
休克实验-家兔失血性休克法
休克实验-大鼠失血性休克法
发热实验-家兔内生致热原性发热法
发热实验-家兔内毒素性发热法
小鼠尾静脉注射方法-小卫星可变重复序列 PCR
小鼠尾静脉注射方法
肾功能不全实验-蟾蜍急性肾功能衰竭法
肾功能不全实验-家兔急性肾功能衰竭法
小鼠解剖取材-【动物剖检】小鼠胸腔检查
小鼠解剖取材-【动物剖检】小鼠腹腔检查
小鼠解剖取材-【动物剖检】小鼠皮下检查
小鼠解剖取材-【动物安死术】小鼠过量麻醉
小鼠解剖取材-【动物安死术】小鼠二氧化碳吸入
小鼠解剖取材-【动物安死术】小鼠麻醉后放血
小鼠解剖取材-【动物安死术】小鼠颈椎脱臼
小鼠解剖取材-【动物影像】小鼠正电子发射型计算机断层显像
小鼠解剖取材-【动物影像】小鼠核磁共振成像
小鼠解剖取材-【动物影像】小鼠超声成像
小鼠解剖取材-【动物影像】小鼠活体光学成像
小鼠解剖取材-【动物影像】小鼠 CT 扫描
小鼠解剖取材-【标本采集】小鼠骨髓采集
小鼠解剖取材-【标本采集】小鼠胸水的采集
小鼠解剖取材-【标本采集】小鼠腹水的采集
小鼠解剖取材-【标本采集】小鼠尾尖采血
小鼠解剖取材-【标本采集】小鼠腹主动脉采血
小鼠解剖取材-【标本采集】小鼠眼眶后静脉丛采血
小鼠解剖取材-【标本采集】小鼠心脏采血
小鼠解剖取材-【标本采集】小鼠颈静脉采血
小鼠给药造模-【动物给药】小鼠脑内注射
小鼠给药造模-【动物给药】小鼠关节腔内注射
小鼠给药造模-【动物给药】小鼠静脉注射
小鼠给药造模-【动物给药】小鼠腹腔注射
小鼠给药造模-【动物给药】小鼠肌内注射
小鼠给药造模-【动物给药】小鼠皮内注射
原代细胞培养和传代培养的方法及其注
常规细胞培养方法（原代培养和传代培养）
常规细胞培养方法
原代培养中成纤维细胞的抑制
悬浮培养工艺与转瓶培养工艺的比较分析
细胞培养从头学
细胞培养完整手册
肺癌细胞SPC-A-1的传代经验
PriCells: Methods of Isolation and Culture of Human Hepatocytes
原代培养人前脂肪细胞
大鼠主动脉内皮细胞的培养
常用原代动物细胞培养——肝星状细胞培养
肝星状细胞培养
大鼠心室肌微血管内皮细胞的原代培养
胚胎小鼠纹状体神经干细胞的分离培养及鉴定
肝细胞原代培养
德发现非基因技术神经干细胞培育新方法
Recipies for Cell Fusion
MDCK细胞培养
细胞冻存和复苏标准操作规程(SOP)
纯化学定义、无异源动物成分的人多能干细胞(hPSC)培养基
按细胞比重分离B细胞
wish细胞的传代
不同实验中 transwell 小室的选择和使用
PriCells: Introduction of Isolation and Culture of Human Primary Hepatocytes
小鼠单核巨噬细胞RAW264.7的消化及传代
R&D Systems支原体污染检测新方法（ELISA）专辑
R&D Systems 适用于神经细胞培养的无血清培养基添加剂
PriCells: 正常人表皮黑色素细胞的培养
小鼠乳腺上皮细胞培养
外周血中嗜碱性粒细胞分离方法
PC12细胞培养
PBS相关资料
家兔椎间盘(intervertebral disc )细胞的体外培养方法
marc 145细胞培养的简单综述
Jurkat细胞（人外周血白血病T细胞）的传代
细胞培养中霉菌污染问题
InSphero 3D微组织—带给你新药测试新发现
如何培养脐静脉内皮细胞（HUVEC）?
HePG2细胞和L-02细胞的传代经验
HepG2细胞培养的相关条件
HepG2细胞培养的条件
悬浮细胞培养方法求心得
BSSub-XF 细胞培养添加剂介绍
Sucrose Fractionation of Castor Bean
B16鼠黑色素瘤细胞培养的经验
我的骨髓间充质细胞传代方法
细胞消化的个人经验
常用培养基配方（七）
原代细胞的培养与建系(二)
293T细胞的培养与传代
293细胞培养技术
Authentication of Cell Lines
293T细胞的培养心得
ibidi：细胞培养、免疫荧光等细胞实验好帮手
肿瘤细胞培养成纤维细胞排除法
How do I decontaminate my tissue culture
原代细胞染色技术
细胞的体外培养检测产品生物性能
细胞株基本知识1：无菌操作基本技术
细胞直接计数
细胞培养常见28个问题解答
细胞培养的经验
PriCells: Isolation of human adipose tissue-derived mesenchymal stem cells (hADSCs)
细胞培养基的选择
Hela细胞传代步骤
细胞培养无菌操作规范
细胞培养基的基本要求
视神经少突胶质细胞体外培养纯化及鉴定
甲醇酵母表达常用溶液及缓冲液的配制
神经胶质细胞传代
PriCells: 正常人羊膜细胞（HAM）原代细胞培养
大鼠前体脂肪细胞的原代培养操作步骤
小鼠给药造模-【动物给药】小鼠皮下注射
小鼠给药造模-【动物给药】小鼠涂布给药
小鼠给药造模-【动物给药】小鼠呼吸道给药
小鼠给药造模-【动物给药】小鼠灌胃给药
小鼠给药造模-【动物给药】小鼠消化道给药
小鼠给药造模-【动物抓取】小鼠的抓取与保定
小鼠饲养繁殖-【动物标记】小鼠剪趾法
小鼠饲养繁殖-【动物标记】小鼠耳孔法
小鼠饲养繁殖-【动物标记】小鼠染色法
TTC 染色实验（TTC）
蔗糖偏好实验
高架十字迷宫实验（Elevated Plus Maze Test）
小鼠饲养繁育-Tet-On/Tet-Off 和 CreER/loxP 诱导系统区别
收到邮寄细胞后的注意事项
Serum Thawing Heat Inactivation
细胞培养注意事项
细胞培养常见问题及其解决
细胞培养常见问题解答(FAQ)
细胞培养常见问题与分析
人脐静脉内皮细胞（HUVEC）培养
培养细胞的细胞生物学
原代细胞的培养方法
细胞培养方法的问答（1）
细胞冷冻保存方法、注意事项
肺癌细胞（人类）消化经验
细胞株的培养、冻存和复苏
猪肺泡巨噬细胞的培养
细胞培养知识和技术
BHK-21细胞传代
CHO细胞表达系统常见问题及解析
细胞培养常见问题及解答
乳鼠心肌细胞培养经验
丁香园网友细胞培养的经验
细胞培养方法的问答（2）
CHO细胞（中国仓鼠卵巢细胞）的传代
乳鼠肝脏细胞原代培养
细胞培养之问题解答
细胞培养目的和用途
人类滑膜原代细胞培养
人PBMC分离和培养
特殊培养法
猪甲状腺细胞原代培养方法
常用培养基配方（二）
MEM培养基说明书中文版
细胞培养常用培养基及基本特性
CHO细胞无血清培养
常用培养基配方（一）
小鼠成肌细胞C2C12和人横纹肌肉瘤A204细胞的消化传代
细胞支原体污染的荧光检测方法
常用培养基配方（四）
常用培养基配方（六）
常用培养基配方（三）
大鼠肺成纤维细胞培养
新生大鼠心肌细胞培养
无蛋白培养基与限定化学成分培养基
常用培养基配方
斑马鱼精子冷冻保存及体外受精的高通量法
大鼠心肌细胞原代培养
由ES细胞衍生神经上皮细胞培养物
组建组织工程化生肌构成物
从老鼠骨髓中制备原代造血细胞培养物用于电穿孔
仓鼠卵母细胞和小鼠胚胎的收集和冷冻保存
三维细胞培养的新进展
大鼠脑皮质微血管内皮细胞的培养
正常滑膜细胞原代培养
DC-CIK细胞的制备方法
CIK细胞的制备方法
天然细胞培养基
3T3成纤维细胞传代
脂肪源干细胞的培养
Cell Culture Protocols
脾脏单个核细胞悬液的制备
常用细胞培养基
Safety Aspects of Cell Culture
细胞培养基的应用选择
Osteoblast Harvest Adult Mice
Abbreviations
胶质细胞培养（astrocyte, oligodendrocyte）
动物淋巴管内皮细胞的分离
细胞培养中的常见污染
Bcap-37和MDA-MB-435细胞的传代
Bel-7402 人肝癌细胞系
vero（非洲绿猴肾细胞)的传代
8 年细胞培养大神的经验指南！
Protocol for Cell Fusion
正常动物肠肥大细胞的培养
How to Make Competent Cells
ES / MEF cell culture and electroporation of targeting construct
小鼠饲养繁育-影响 CDX 荷瘤效果的因素
小鼠饲养繁育-基因鉴定篇：PCR 鉴定常见问题
小鼠饲养繁育-重度免疫缺陷品系 NCG 的繁育问题
小鼠饲养繁育-免疫缺陷品系裸鼠的繁育问题
小鼠饲养繁育-代谢品系 DIO 繁育常见问题
小鼠饲养繁育-代谢品系 NOD/LtJGpt 繁育常见问题
小鼠饲养繁育-代谢品系 BKS-DB 繁育常见问题
小鼠饲养繁育-C57BL/6JGpt 品系问题
小鼠饲养繁育-动物管理篇：小鼠常见异常行为
新物体识别
动物活体光学成像-发光检测实验
动物活体光学成像-动物发光标记法
动物活体光学成像-常规注射方式——皮下注射、肌肉注射、静脉注射、动脉注射
动物活体光学成像-模式动物的麻醉操作与处死方法
动物活体光学成像-动物实验-小鼠活体光学成像
切除伤口夹板模型-小鼠仿人切除伤口夹板模型
小鼠行为学实验-小鼠抓力测试
小鼠行为学实验-小鼠爬杆实验
小鼠行为学实验-小鼠转棒实验
单糖发酵试验
氨基转移反应的定性鉴定
明胶酶谱实验-
掌叶防己碱的提取与分离及延胡索乙素制备-溶解度差异法
虎杖中游离羟基蒽醌成分的提取和分离-萃取法
皂苷的鉴别-比色法
酒剂、酊剂的制备实验-浸渍法
颗粒剂的制备实验-感冒退热颗粒剂的制备
流浸膏、浸膏及煎膏的制备实验-渗漉法
溶液型液体药剂的制备实验-溶解法
散剂的制备实验-示例法
汤剂、中药合剂及口服液的制备实验-煎煮法
有机氯杀虫剂的残留分析实验-气相色谱-电子捕获检测器（GC-ECD）
反应时间对产物形成的影响实验-二硝基水杨酸法
pH 值、温度、抑制剂对蔗糖酶活性的影响-二硝基水杨酸法
平衡盐溶液配制实验-BSS 的配制实验
天然培养基配制实验-胶原的配制
天然培养基配制实验-水解乳蛋白的配制
天然培养基配制实验-鸡胚浸出液的制备
天然培养基配制实验-鸡血浆的制备
合成培养基配制实验-无血清培养基的制备
合成培养基配制实验-合成培养液的配制
组织培养常用液的配制实验-pH调整液的配制实验
组织培养常用液的配制实验-胰蛋白酶溶液的配制法
组织的分离实验-EDTA 消化分离法
组织的分离实验-离心分离法
组织的分离实验-胶原酶消化分离法
组织的分离实验-胰蛋白酶消化消化分离法
组织的分离实验-机械分散法
血糖测定实验-酶法
总糖含量的测定实验-苯酚-硫酸法
酪蛋白的制备实验-等电点沉淀法
琥珀酸脱氢酶及丙二酸的抑制作用实验-甲烯蓝脱色法
药用植物过氧化物酶同工酶分析及活性比较实验-聚丙烯酰胺凝胶电泳法
谷丙转氨酶的活性测定实验-显色法
离子交换法制备肝素实验
mRNA 的分离实验-oligo（dT）-纤维素亲和层析法
有机磷农药残留测定实验
氨基甲酸酯杀虫剂的残留分析实验
拟除虫菊酯杀虫剂的残留分析实验
大米中除草剂残留量分析实验-大米中除草剂残留量分析技术
测定小麦中均三氮类除草剂和取代脲类除草剂残留量实验-测定小麦中均三氮类除草剂和取代脲类除草剂残留量
测定植物性产品中苯氧後酸类除草剂残留量实验-GC-MS/MS法
藻类多糖的结构与生物活性研究实验-高效液相层析（HPLC）法
粮谷及油籽中酰胺类除草剂残留量测定实验-GC-ECD法
糙米中有机氯农药拟除虫菊酯和多氯联苯残留量测定实验-凝胶渗透色谱及固相萃取净化技术
蔬菜中多种农药残留测定实验-气相色谱质谱联用法
测定氨基糖苷类抗生素的残留量实验-HPLC-MS/MS法
测定鱼和肉中大环内酯类抗生素残留量实验-HPLC-ESI-MS-SIM法
测定肝脏或肌肉中爱普菌素、阿维菌素、多拉菌素和伊维菌素残留量实验
动物源食品中四环素类兽药多残留测定实验-高效液相色谱法
测定动物组织中氯霉素及其相关化合物残留量实验-气相色谱-质谱法
动物组织中喳诺酮类残留量的测定方法实验-高效液相色谱法
测定鸡肉、猪肉中硝基咪唑类药物及其代谢物残留量实验-气相色谱/质谱检测法
测定鸡肉、猪肉中硝基咪唑类药物及其代谢物残留量实验-HPLC法
测定动物源性食品中硝基呋喃代谢物残留量实验-HPLC-MS/MS法
测定动物源性食品中激素残留量实验-HPLC-MS/MS法
测定动物源食品中甲状腺抑制剂残留量实验-气相色谱-质谱法
蛋白质与核酸的紫外交联实验-用溴脱氧尿苷（BrdU)取代的探针进行紫外交联
酵母菌基因组转座子的诱变实验-mTn诱变基因产物的表位标记
酵母菌基因组转座子的诱变实验-小载体聚合酶链反应
酵母菌基因组转座子的诱变实验-基本方案
酵母菌载体与克隆基因表达产物的检测
放射自显影原位杂交玻片的复染和压片固定实验-甲苯胺蓝染色
放射自显影原位杂交玻片的复染和压片固定实验-苏木精/伊红染色
放射自显影原位杂交玻片的复染和压片固定实验-姆萨 (Giemsa) 染色
用非同位素探针进行原位杂交和检测实验-地高辛标记探针的信号放大
用非同位素探针进行原位杂交和检测实验-杂交信号的放大
用非同位素探针进行原位杂交和检测实验-荧光原位杂交
细胞凋亡的形态：生化性质实验-吖啶橙或溴化乙锭染色
细胞凋亡的形态：生化性质实验-吖啶橙染色
细胞凋亡的形态：生化性质实验-使用活体荧光染料的显微镜定量凋亡指数和细胞活力（台盼蓝染色）
β-半乳糖苷酶活性的整体封片免疫组化检测实验-准备大组织的厚切片进行β-半乳糖苷酶染色
β-半乳糖苷酶活性的整体封片免疫组化检测实验-半乳糖苷酶活性的整体封片染色和免疫组化检测
核酸透射电镜样品制备实验-核酸样品
乙醇脱氢酶的测定实验-氧化反应测定
乙醇脱氢酶的测定实验-还原反应测定
L-乳酸脱氢酶测定实验-氧化反应测定
L-乳酸脱氢酶测定实验-荧光光度计法测定还原反应
L-乳酸脱氢酶测定实验-分光光度计法测定还原反应
过氧化物酶的测定实验-供体：联二茴香胺
如何选择细胞培养板
细胞是有感觉的
Design and Equipment for the Cell Culture Laboratory
The Cell Environment (including types of culture medium)
Cell Cultures
细胞培养基的保存
神经胶质细胞传代经验
Equilibrium Density Gradient - Percoll
20°C下配制的缓冲液，在较高或较低的温度下PH值会改变吗？
细胞培养技术
PC12细胞培养及实验设计
我们实验室细胞传代的一般方法
细胞培养中的一些试剂
原代神经元培养
原代胎儿肾小球系膜细胞培养
PriCells: Isolation, culture, and purification of Human Pulmonary Microvascular Endothelial Cells
Quality Control Considerations
3T3-L1细胞诱导分化
蛋白marker发展史
转铁蛋白应用
bca法测蛋白浓度步骤
考马斯亮蓝染色原理
PriCells: 正常小鼠腹股沟脂肪垫获取脂肪源干细胞
人类原代滑膜细胞传代
基本培养基的配制及细胞培养的条件
鼠肝细胞原代培养实验方法
脐静脉内皮细胞培养
自行配制DMEM的方法及说明书
SDS-聚丙烯酰胺凝胶电泳检测外源蛋白的表达
蛋白质的SDS-聚丙烯酰胺凝胶电泳实验
蛋白质凝胶电泳的操作方法
SDS聚丙烯酰胺凝胶电泳法—蛋白质的分子量测定
聚丙烯酰胺凝胶圆盘电泳
SDS-PAGE常见问题分析
双向电泳各步骤注意事项
蛋白等电聚焦凝胶电泳技术
聚丙烯酰胺凝胶选择指导
实验技巧：SDS－PAGE 电泳常见问题及指南
SDS-PAGE原理
等电聚焦
电泳概述
聚丙烯酰胺凝胶电泳PAGE胶的配制
固相pH梯度等电聚焦分析讨论
SDS-PAGE电泳过程中常见问题以及解决方法
SDS-PAGE常用参数
SDS-PAGE胶的干燥
聚丙烯酰胺凝胶电泳
SDS-PAGE实验操作
蛋白质双向电泳过程与体会
SDS-PAGE胶染色
SDS-聚丙烯酰胺凝胶电泳法测定蛋白质的分子量（垂直板型电泳）
过氧化物酶同工酶聚丙烯酰胺凝胶电泳
SDS－聚丙烯酰胺凝胶垂直板电泳分离蛋白质
等电聚焦电泳法测定蛋白质的等电点
SDS-PAGE测定蛋白质分子量及蛋白质的纯度鉴定
表达蛋白的SDS-聚丙烯酰胺凝胶电泳分析
等电聚焦电泳介绍
质谱兼容的银染
Comassie Stain of Protein Gel
Protein Electrophoresis
Silver Staining Protocol
聚丙烯酰胺凝胶层析的技术数据
Silver Staining of SDS-PAGE Gels(Ⅰ)
Analysis of total E.coli protein by SDS PAGE
Analysis of total E. coli protein by SDS PAGE
SDS-PAGE
常用蛋白质分子量标准参照物
SDS-PAGE Gels（SDS-聚丙烯酰胺凝胶电泳）
即时可用的蛋白质电泳标准
重组大肠杆菌目的蛋白的表达-蛋白电泳
过氧化物酶的测定实验-供体：愈创木酚
过氧化物酶的测定实验-供体：ABTS
二氢硫辛酰胺脱氢酶的测定实验-硫辛酰胺的还原反应
二氢硫辛酰胺脱氢酶的测定实验-二氢硫辛酰胺的氧化反应
脂肪酶的测定实验-荧光分析法
脂肪酶的测定实验-pH 稳定计（自动滴定器）法
碱性磷酸酶的测定实验-哺乳动物碱性磷酸酶
神经细胞培养基质的预处理实验
蛋白酶的测定实验-Anson 测定法
两种不同的总蛋白提取方法总结
胞嘧啶的性质及与胸腺嘧啶的区别
石蜡切片免疫组化步骤及所需生化试剂列表
α-葡糖苷的测定实验-测定 α-葡糖苷酶
α-葡糖苷的测定实验-测量葡萄糖
蛋白酶的测定实验-茚三酮测定法
蛋白酶的测定实验-偶氮酪蛋白测定法
蛋白酶的测定实验-酪蛋白测定法
脲酶的测定实验-光度计测定法
脲酶的测定实验-pH 稳定计法
尼氏染色实验
LFB 髓鞘染色法
神经系统束路追踪实验
单纤维记录实验-单纤维记录实验
胰凝乳蛋白酶-用 GLUPHEPA 测定
胰凝乳蛋白酶-用 SUPHEPA 测定
在体动物的细胞外记录实验
细胞内记录实验
培养细胞膜片钳记录实验
异构酶的测定实验-葡萄糖异构酶微板测定
异构酶的测定实验-葡萄糖异构酶的测定实验
异构酶的测定实验-D-木糖异构酶微板分析
异构酶的测定实验-D-木糖异构酶测定
神经组织块膜片钳全细胞记录实验
由酶循环确定烟酰胺核苷酸实验-NAD（H）的测定
由酶循环确定烟酰胺核苷酸实验-NADP（H）的测定
组织薄片膜片钳全细胞记录实验
细胞内钙成像实验
蛋白质与溴化氰活化的琼脂糖结合实验
二氨基己基间隔物的结合实验
纤维素的高碘酸活化实验
抗体或蛋白质中硫基的引入实验
蛋白质与二甲基辛二亚酰胺的交联实验
酶在非孔玻璃表面的共价固定化实验-基本方案
脊髓机械性损伤模型的制作实验-大鼠脊髓全横切模型
脊髓机械性损伤模型的制作实验-大鼠脊髓半横断模型
脊髓机械性损伤模型的制作实验-大鼠脊髓挤压伤模型
脊髓机械性损伤模型的制作实验-大鼠脊髓NYU模型
脊髓机械性损伤模型的制作实验-实验前的准备及暴露脊髓
碱水解和由二吲哚碳酰的活化实验-基本方案
脊髓损伤后的运动功能学评价实验-斜板试验(inclined plane test)
脊髓损伤后的运动功能学评价实验-步态分析(gait analysis)
脊髓损伤后的运动功能学评价实验-旷场试验(open field test)BMS评分
脊髓损伤后的运动功能学评价实验-旷场试验(open field test)BBB评分
视神经损伤模型实验-荧光金逆行性标记（上丘及外侧膝状体）
视神经损伤模型实验-坐骨神经移植及荧光金逆行性标记
视神经损伤模型实验-视神经横断模型及荧光金逆行标记
线栓法制备大鼠大脑中动脉栓塞 (MCAO) 模型-基本方案
帕金森病模型的制作实验-基本方案
锂－匹罗卡品大鼠癫病模型制作实验-基本方案
神经系统免疫性疾病模型制作实验-实验性自身免疫性重症肌无力(EAMG)模型
实验性自身免疫性神经炎(EAN)模型
神经系统免疫性疾病模型制作实验-实验性变态反应性脑脊髓炎(EAE)模型
慢性不可预知温和应激 (CUMS) 模型实验-慢性温和不可预测性应激模型实验（CUMS）
慢性不可预知温和应激 (CUMS) 模型实验-基本方案
疼痛实验动物模型制作实验-疾病并发慢性痛模型
疼痛实验动物模型制作实验-组织炎症疼痛模型
疼痛实验动物模型制作实验-神经病理痛模型(neuropathic pain model)
自由基显示实验-细胞化学法
自由基显示实验-H2O2细胞化学法
常用痛行为学检测实验-痛厌恶情绪的检测方法
常用痛行为学检测实验-触刺激诱发痛(allodynia)
常用痛行为学检测实验-痛觉过敏 (hyperalgesia)
常用痛行为学检测实验-自发痛(spontaneous pain)
分光光度计测定溶液的吸光值-分光光度计测定溶液的吸光值
PPO 活性测定-分光光度法
碳酸氢钠、次碳酸铋散（西皮氏散 II 号）的制备实验-散剂药物制备法
糖的硅胶 G 薄层层析实验-薄层层析
维生素 B2 (核黄素) 荧光测定法实验-荧光测定法2
维生素 B2 (核黄素) 荧光测定法实验-荧光测定法-1
温度和 pH 对唾液淀粉酶活性的影响实验-碘液显色法
血清蛋白醋酸纤维素薄膜电泳的若干问题及解决方法
醋酸纤维素薄膜电泳操作要点及常见差错和失败原因分析
醋酸纤维薄膜电泳分离血清蛋白
血红蛋白电泳（hemoglobin electrophoresis）
醋酸纤维素薄膜电泳分离血清蛋白质并定量
血清蛋白醋酸纤维薄膜电泳
蛋白质测定方法之双缩脲法（Biuret法）
蛋白测定方法之Folin—酚试剂法（Lowry法）
羧肽酶的制备及其性质
高通量法全局研究酿酒酵母细胞器形态学
利用酿酒酵母定量蛋白质组学鉴定蛋白质复合物
蛋白质泛素化检测
血清总蛋白质的测定方法
蛋白质的性质实验:蛋白质和氨基酸的呈色反应
四种紫外吸收法测蛋白质含量
组织和细胞总RNA提取（TRIzol法）
RNA抽提之应知应会
蛋白质含量测定法
紫外吸收法测蛋白质含量
蛋白质浓度测定：紫外分光光度法
植物体内可溶性蛋白质含量的测定(福林－酚法的原理）
高效液相色谱之高效排阻液相色谱
离子交换色谱
原位微量BCA蛋白定量法
酵母核糖核酸的提取、水解及组成成分的鉴定
血浆游离血红蛋白测定
使用可逆性生物素化测量质膜蛋白胞吞速率
稳定性同位素及其应用领域
血清中COMP检测及临床意义
P53蛋白检测
Folin-酚法测定蛋白质含量
蛋白质定量
蛋白质的透析
牛乳中蛋白质的提取与鉴定
双缩脲法（biuret法）测蛋白质含量
考马斯亮兰法（bradford法）蛋白质含量测定
考马斯亮兰法（Bradford法）测定蛋白浓度
folin—酚试剂法（lowry法）测蛋白质含量
改良的简易folin—酚试剂法测蛋白质含量
测定蛋白质含量之常规方法介绍
6种方法测定蛋白质含量
植物体内可溶性蛋白质含量的测定(LoWry法与劳里法）
蛋白质含量测定的方法(Bradford 方法)
蛋白质的化学复习笔记
BCA蛋白质定量检测
影响紫外吸收法测定蛋白质/DNA浓度的因素
Quantitation of Protein: Coomassie Bright Blue Dye - binding Method (Bradford Method)
蛋白质技术之“蛋白质浓度测定（一）”
Bicinchoninic Acid (BCA) Protein Assay (Smith)
Bradford Protein Assay
Brandford法测定蛋白浓度
蛋白功能验证的方法
蛋白纯化策略
一个了解蛋白谱（protein profiling）的完整的解决方案
定量蛋白质组学的方法学介绍
蛋白质、多肽提取分离
酵母蛋白的提取方法
实验室蛋白常用试剂配方
膜蛋白分离的方法和原理
标记亲和素生物素法 (BA-ELISA) 实验-检测未知抗体
标记亲和素生物素法 (BA-ELISA) 实验-检测未知抗原
快速筛选测定浓缩苹果汁中 105 种农药残留量实验
测定水果和蔬菜中 446 种农药多残留实验
测定动物源食品中14 种β-内酰胺类兽药残留量实验
测定动物源性食品中 22 种磺胺类残留量实验
测定肝、肾和肉中 b-受体激动剂残留量实验
尿喀啶干扰分析法
甲基化干扰分析法
DNA 酶 I 足迹分析法实验-粗制品的DNA酶足迹分析法
DNA 酶 I 足迹分析法实验-用光密度及数值分析法决定蛋白质结合的平衡常数
DNA 酶 I 足迹分析法实验-DNA酶I足迹分析法
编码 DNA 结合蛋白的 cDNA 克隆的检测、纯化和鉴定-从重组溶源菌中制备粗提物鉴定DNA结合蛋白的活性
编码 DNA 结合蛋白的 cDNA 克隆的检测、纯化和鉴定-用盐酸胍进行干燥膜的变性/复性循环
编码 DNA 结合蛋白的 cDNA 克隆的检测、纯化和鉴定-用位点识别DNA筛选λgtll表达文库
用克隆化基因在体外合成的蛋白质分析 DNA-蛋白质相互作用
序列特异性 DNA 结合蛋白的亲和层析纯化实验-DNA亲和层析法
序列特异性 DNA结合蛋白的亲和层析纯化实验-用制备性凝胶电泳纯化寡核苷酸
序列特异性 DNA 结合蛋白的亲和层析纯化实验-DNA偶联于溴化氰活化的琼脂糖上
序列特异性 DNA 结合蛋白的亲和层析纯化实验-DNA亲和介质的制备
将 DNA 导入酵母细胞实验-单链高分子质量载体DNA的制备
将 DNA导入酵母细胞实验-电穿孔转化
将 DNA 导入酵母细胞实验-乙酸锂转化
荧光素酶的测定实验
merck(Sigma-Aldrich BioReliance Millipore SAFC Milli-Q Supelco)品牌介绍和办事处联系方式
电极缓冲液中甘氨酸的作用
石蜡切片免疫组化步骤及所需生化试剂列表
LB培养基和MS培养基的比较与区别
我国乳业首个乳铁蛋白生产获批，飞鹤科研创新里程碑
6月1日起，这些酒不能再称之为“白酒”
LIMS到底能给实验室带来多大好处？
蜂产品生产许可审查细则（2022版）》来了！
益生菌食品新团标发布：活菌率必须达到50%才满足1级标准
LIMS系统能带来哪些变化和提升？
QC人注意了！GMP拒绝了你的“QA取样，QC化验”模式
低温奶迎来发展新机遇！两部门发文推动冷链物流服务网络向农村延伸
全国首例“COD去除剂”干扰环境监测设施刑事案宣判
检测分包管理程序
北京点名的3家“问题”实验室结局：批捕，吊销执照
中纪委深度关注：第三方核酸检测如何“保质保量”
关于特种设备检测机构核准等有关事项的通知
LIMS在高校实验室的应用
第三方检测认证再迎利好
食品企业过敏原管理与控制（从研发到出厂）
《检验机构认可领域分类》7月1日起发布实施
全国首例消防安全领域危险作业案（非事故类行刑衔接案件）成功判决。
你知道LIMS系统中的电子记录该如何管理吗？
如何利用感官来选购白酒？
新冠病毒实验室检测技术指南
选择LIMS系统要具有哪些优势？
【通关监管】对含大麻食品化妆品说“不”！
洁净室（无菌室）的使用和管理
食品出厂检测，你了解多少？
干货丨一招解决实验室不符合项整改问题
干货丨标准物质的期间核查等实验室典型问题的解答
实验室记录如何编写？有什么要求？
分享｜实验室非常危险的14种试剂
微生物检测实验室的质量控制措施
干货丨食品添加剂“配料带入”与“本底带入”的要点分析
干货丨如何轻松使用pH计？
食品安全抽检抽样流程及注意事项
质量检验分类
各种实验室温度的控制要求
实验室生物安全管理制度
Western Blot 的注意事项
蛋白组学----ITRAQ技术简述
蛋白质提取与制备
Western Blot详解－常见的问题指南（二）
Western Blot详解－常见的问题指南（三）
蛋白质组学同位素标记的亲和标签方法
ABIGEN实验方法
纯化全长/高纯度蛋白方法（ Double-tag）
肾组织提总蛋白的方法
AKTA快速蛋白分离系统和HPLC 的优劣比较
seldi技术介绍
高通量蛋白质组学新时代——iTRAQ技术
Westernblot实验步骤及注意事项
免疫印迹法
Western Blot详解－原理
经典的western方法
电泳和Western转印的常见问题
转录组学和蛋白组学研究
糖尿病相关勃起障碍治疗分子靶标筛选
组织/细胞RNA快速提取
大肠杆菌菌体蛋白残留量（E.coli DH-5α）酶联免疫分析
大鼠血管紧张素1-7（Ang 1-7)酶联免疫分析（ELISA）
大鼠磷酸化蛋白激酶C（P-PKC）酶联免疫分析
蛋白浓缩方法基本
ＷＥＳＴＥＲＮＢＬＯＴＴＩＮＧ心得
蛋白质的纯化
蛋白质特性与分离纯化技术的比较和选择
新一代蛋白研究工具——抗体芯片
R&D Systems 蛋白芯片技术专辑
多肽合成历史
蛋白质组学及研究技术路线
芯片分离蛋白
蛋白质PEG化修饰与纯化
酶的分离纯化方法简介
FIH蛋白可控小鼠体重研发减肥药物新思路
牛血清淀粉样蛋白A（SAA）酶联免疫分析（ELISA）
TCA沉淀方法
western blot试验心得
犬免疫球蛋白M(IgM)酶联免疫分析（ELISA）
犬C-反应蛋白(CRP)酶联免疫分析
犬乳腺癌抗原15-3（CA15-3）酶联免疫分析（ELISA）
人高迁移率族蛋白B1(HMG-B1)酶联免疫分析（ELISA）
人磷脂酰肌醇蛋白聚糖3（GPC-3)酶联免疫分析
人血管内皮钙粘蛋白(VE-Ca)酶联免疫分析（ELISA）
人血栓调节蛋白(TM)酶联免疫分析（ELISA）
人β2-微球蛋白（β2-MG）酶联免疫分析（ELISA）
人层粘蛋白(LN)酶联免疫分析
人正五聚蛋白-3（PTX3）酶联免疫分析（ELISA）
人CREB结合蛋白(CBP)酶联免疫分析（ELISA）
人β淀粉样蛋白1-40（Aβ1-40）酶联免疫分析（ELISA）
权威解答丨关于食品安全抽检的问题，总局答复合集
关于霍乱，这些知识你要知道
【收藏】洁净区空间消毒灭菌常用的杀孢子剂
实施“6S”管理提升药检实验室管理水平
100个实验室常用标准，你想找的都在这里了！
市场监管总局等4部门发布《查处生产经营含金银箔粉食品违法行为规定》
高纯试剂可以当标准物质使用吗？
公司内审、管理评审常见问题点及应对策略！
CNAS扩项需要提交的资料清单
LIMS在现代化食品检验中的重要应用
环境监测和环境检测，到底有什么区别？
资质认定和实验室认可的关系
能力验证相关术语和定义
可以套用的作业指导书编写方法！
人β淀粉样蛋白1-42（Aβ1-42）酶联免疫分析（ELISA）
人蛋白激酶C (PKC)酶联免疫分析
人核基质蛋白22（NMP-22）酶联免疫分析（ELISA）
人基质金属蛋白酶-13（MMP-13）酶联免疫分析
人基质金属蛋白酶-2（MMP-2）酶联免疫分析（ELISA）
人基质金属蛋白酶-3（MMP-3）酶联免疫分析（ELISA）
人基质金属蛋白酶-9（MMP-9）酶联免疫分析（ELISA）
人结合珠蛋白/触珠蛋白前体(pre-Hpt/HAP)酶联免疫分析（ELISA）
人金属蛋白酶组织抑制因子-1(TIMP-1)酶联免疫分析（ELISA）
人硫氧还蛋白相互作用蛋白(TXNIP）酶联免疫分析（ELISA）
人免疫球蛋白G Fc 片段(Fcγ)酶联免疫分析（ELISA）
人尿微量白蛋白（mALB)酶联免疫分析（ELISA）
人视黄醇结合蛋白4(RBP-4)酶联免疫分析（ELISA）
人水通道蛋白4（AQP4）酶联免疫分析（ELISA）
人水通道蛋白4抗体（AQP4 Ab）酶联免疫分析
人胃蛋白酶原（PG）酶联免疫分析（ELISA）
人血清淀粉样蛋白A（SAA）酶联免疫分析（ELISA）
人血小板膜糖蛋白IIbIIIa(GP-IIbIIIa/CD41+CD61)抗体
人胰岛素样生长因子结合蛋白-3(IGFBP-3)酶联免疫分析（ELISA）
人脂蛋白相关磷脂酶A2(Lp-PL-A2)酶联免疫分析（ELISA）
蛋白质组鉴定技术简述
可视蛋白质组学
如何选择凝胶
Western Blot详解－常见的问题指南（一）
使用ProteOn XPR36蛋白相互作用阵列系统
透析袋和透析膜处理方法
酵母双杂交系统的发展和应用
蛋白质组学研究的一般工具与方法
膜蛋白研究的整体解决方案
兔载脂蛋白E（APO-E）酶联免疫分析（ELISA）
质谱蛋白质组学在微生物鉴定中的应用
蛋白质微阵列技术
蛋白质相互作用组学分析技术
关于细菌超声破碎的小总结
SWATH技术——蛋白质组学的又一项革命
如何使用透析袋
论什么才是一个好的 label-free 设计方案？
小鼠干扰素诱导蛋白10（IP-10）酶联免疫分析（ELISA）
小鼠卵白蛋白特异性IgG抗体(OVA-sIgG)酶联免疫分析（ELISA）
标准物质使用管理说明
实验室标准物质购买指南：如何选择与购买适合你的标准物质
PCR新手必看！细节制胜
采用 qRT-PCR 检测细胞炎性因子
食品保质期的法律规制
做审核员必读的十条审核规则！你做到了吗？
抽样检验的标准程序是什么？
啥是盲样考核？该如何准备？
快速溶剂萃取的原理及技术要点
微生物检测结果不准确原因分析
分光光度计测量误差四大原因
环境监测表面取样必须有棉签擦拭取样吗？
实验室各类期间核查，你做全了吗？
实验室印章印签管理程序
分享丨“内标法”相关知识点汇总
紫外分光光度计为什么要校正？如何校正？
菌落总数的错误操作
食品安全标准到底包括哪些内容？
各种检出限及区别
化验室剧毒化学品管理制度，你的管理有漏洞吗？
实验误差，控制和消除的方法
我们为什么需要LIMS？
医用手套的分类、生产与检测要求
食品检测前处理方法、基本要求、净化技术及前处理原则
10类实验室试剂保管要求
实验室废水废气处理措施
实验室玻璃器皿高效管理！值得学习
实验室管理体系、质量控制、方法开发，这些明白了你也可以成为管理者！
表格版|液相色谱法方法验证操作详解，手把手教你液相色谱方法验证！
别小瞧，这一根小小的移液管！
一个标准的实验室配置应该是这样的
标准溶液的正确使用、配制、保存、要求你都会了吗？
GB 2760食品添加剂使用标准修订变化速览
小鼠心肌肌钙蛋白I（cTnI）酶联免疫分析
转录分析的5种方法
蛋白质的蛋白质的提取技术
SWATH技术解决方案及应用
一个正在崛起的新学科——蛋白质组学
WB操作规程
Western bloting 技术
卵清蛋白分离提取
亲和层析纯化胰酶
细胞裂解液的制备
蛋白质组技术
等电聚焦电泳（IEF）分离蛋白及测定蛋白质等电点
多酚氧化酶（PPO）的分离提取
胰酶分离提取
单细胞凝胶电泳标准操作规程(本室SOP)
功能蛋白质组学研究的有力工具：Ettan DIGE荧光差异蛋白表达分析系统
聚乙二醇修饰反应类型
蛋白质组分析中的初步分离
使用胶做western blot的方法
蔗糖酶的提取
Western bloting 综述
2套多肽固相合成装置的设计与应用
双向凝胶电泳分析小鼠子宫内膜胚泡着床点和着床旁组织蛋白质组
猪免疫球蛋白G2a(IgG2a)酶联免疫分析（ELISA）
Western Blot详解－主要试剂
Western 不同转膜方法的取舍
丙烯酰胺胶中肽提取和蛋白消化技术Digestion of Proteins and Extraction of Peptides from an Acrylamide
Small Scale GST-Tag Purification Under Nature Conditions
蛋白质组学-质谱分析
Preparation of Brain Membrane Fractions for Western Blot Analysis
Western Blot
Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots
美国实验室wetern方法
Affinity chromatography (with HIS-tagged proteins)
WESTERN PROTOCOL
蛋白酶抑制剂资料 Protease Inhibitors
HISTONE KINASE ASSAY
Isolation of Non-muscle Actin
Western Blot/Tissue Preparation
一步法甲醛胶连检测蛋白质相互作用Protein Interactions Captured by Chemical Cross-linking: One-Step Cross-linking with
Protein extraction from whole tissues for IEF
Western Blotting with Monoclonal Antibodies
GST Fusion Protein Prep
GSP融合蛋白的准备 GST Fusion Protein Prep
蛋白质和多肽MALDI-MS分析方法 General Method for MALDI-MS Analysis of Proteins and Peptides
Protein Expression and Purification Protocol
蛋白复合体直接酶消化法Direct Enzymatic Digestion of Protein Complexes
Home-made Taq Polymerase Purification
ELISA FOR DETECTING SECRETED ICAM-1
WESTERN BLOTTING USING CHEMILUMINESCENCE
Small scale His-Tag fusion protein purification under denaturative conditions
显微镜日常维护和保养
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
检出限，定量限，仪器检出限，方法检出限，别傻傻分不清了。
分享丨实验室非常危险的14种试剂
分享丨流动相配制过程中应该避免的10个坏习惯！
Western Blot Protocols
Protocol for anti-HA antibody Western Blotting
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
运用肽库筛选磷酸化激酶motif方法 Screening Kinase Phosphorylation Motifs Using Peptide Libraries
Synthesis and Probing of Membrane-bound Peptide Arrays
分享丨实验室样品管理做得好，90%的问题能避免
氰化高铁血红蛋白测定法
动物血中超氧化物歧化酶的提取
外源基因的诱导表达
15种蛋白质单晶培养的方法
原核表达-Novagen产品
Double-tag在蛋白纯化过程中的应用
微生物实验室标准化操作规程！
【实验技巧】WB 上样前如何制样
关于原核表达载体
蛋白质组学入门问题FAQ
【取样】无菌取样技术汇总贴！
奶酪、奶酪棒……正在走进我们每天的生活
绿色荧光蛋白在抗肿瘤药物研究中的应用
Pichia酵母表达系统使用心得
【实验技巧】丽春红染色
静脉用免疫球蛋白在神经系统疾病中的应用
蛋白质的表达、分离和纯化
关于牛奶的那些小知识~
植物基因在大肠杆菌中的原核表达
原核表达：遇到瓶颈怎么办
同样是做实验，为什么有的人效率很高，有的人需要加班？如何高效做有机实验
人类蛋白质组表达谱蛋白质鉴定的分步搜索策略
【化学科普】118种化学元素的名称是咋来的？
His Tag的功与过
His-Tag原核表达――Qiagen
实验室超纯水机的保养方法
原核表达条件优化
小仪器有大学问温度计发展史探究
实验室离心机事故的原因分析
原核表达--宿主菌株选择指南
我们的化验室是这样布局的
表达蛋白的生物学活性的检测
食品中农药残留的检测方法汇总
种子蛋白质系统分析：种子蛋白质组分分析
Cas9 蛋白过表达慢病毒包装
在大肠杆菌（E.Coli）表达蛋白产物
原核表达实验方法
最新融合表达的亲和纯化小标签StrepTaq
毕赤酵母表达系统在外源基因表达中的研究进展及应用
蛋白的裂解与提取方法步骤
体内体外表达――Promega
Determination of target protein solubility
Bacterial Expression of Motors
单克隆抗体特异性识别的野生型谷胱甘肽-S-转移酶进展
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Invitro transcription with yeast nuclear extract
Protein Expression and Purification of RPA70-AB (181-422)
大范围微管蛋白的制备
常用核酸和氨基酸代码
蛋白质凝胶电泳凝胶的制备
Native-PAGE常见问题分析
Native-PAGE实验方法
Native-PAGE和SDS-PAGE的比较
Native-PAGE的基本分类
非变性聚丙烯酰胺凝胶电泳(Native-PAGE)的分类
非变性胶蛋白电泳
血清蛋白琼脂糖凝胶电泳
双向电泳溶液配制方法
DIGE荧光差异凝胶电泳技术特点及应用
抑郁大鼠海马的双向凝胶电泳图谱分析
生物化学实验常用技术:电泳技术
等电聚焦[isoelectric focusing]
电泳技术
植物过氧化物酶同工酶测定
双向电泳操作步骤
双向电泳的样品的制备原则及步骤
双向电泳完整操作步骤
琼脂糖凝胶电泳法分离LDH同工酶及血清LDH总活力的测定
双向电泳操作步骤及相关溶液配置
硝酸银染色
肿瘤琼脂糖凝胶电泳标本的拍摄方法
天麻蛋白质的双向电泳和肽质量指纹谱分析与鉴定
急性脊髓损伤蛋白质的双向电泳分析和质谱鉴定
Sample preparation (analytical gels)
2D Gel Electrophoresis
钠，钾-三磷酸腺苷酶活性测定
Western Blot试验宝典
X射线晶体成像技术测定蛋白质结构
亚硝酸法测定超氧化物歧化酶同工酶活性
化学发光免疫法测定超氧化物歧化酶同工酶活性
大肠杆菌蛋白表达纯化策略
蛋白纯化些常见问题总结
优化生物工艺研发的创新性技术--定量分析蛋白聚集体
考马斯亮蓝法测定蛋白质浓度的原理、步骤及注意事项
谷胱甘肽-S-转移酶活性测定
化学发光法测定超氧化物歧化酶活性之一
TNF-α生物学活性检测方法
极谱氧电极法测定超氧化物歧化酶活性
酵母双杂交的实验步骤【分享】
拉曼在制药及药检中的应用
改良邻苯三酚法测定超氧化物歧化酶活性
总蛋白和膜蛋白提取方法
膜蛋白提取方法
肾上腺素自动氧化测定超氧化物歧化酶活性
免疫沉淀技术的原理和抗体选择
肽的溶解性及溶解方法
细胞色素b5的测定
细胞色素P-450的光谱测定
NI-NTA蛋白提纯的原理、步骤与常遇问题分析
影响蛋白超滤的因素以及使用超滤膜的注意事项
蛋白测序的原理、步骤、以及N端测序服务的注意事项
货比三家,教你选对SDS-PAGE预制胶设备
凯氏定氮法测定蛋白质的含量
SDS-PAGE检测蛋白表实验操作步骤
双缩脲试剂测定蛋白质含量
免疫组化实验过程中的要点和技巧
Western Blot免疫印迹实验操作方法
高通量多肽阵列技术的应用与主要特点
各种各样的蛋白标签从容应对蛋白实验
斐林试剂测定还原糖
原核表达操作步骤及注意事项
甘氨酸的作用
酪氨酸的作用
脯氨酸的作用
通用电气Biacore——蛋白质相互作用研究好帮手
美发明新型固体NMR法检测蛋白结构
影响外源基因在巴氏毕赤酵母中表达的因素
酵母表达外源蛋白
默克公司关于HIS*TAG实验过程中常见问题与解决方法
Native-PAGE电泳的注意事项
外源基因在原核细胞中的诱导表达
蛋白质非共价复合物的电喷雾质谱(ESI
全能核酸酶BENZONASE NUCLEASE
免疫检测的基础知识介绍
Folin—酚法测定血清蛋白的含量
苏氨酸在饲料添加及营养上的用途
Pierce推出更快更彻底的新品去垢剂
TRIzol法同时提取RNA,DNA,蛋白质
Western Blot的原理和所用试剂汇总
氨基酸的生理作用
各种氨基酸在生命代谢活动中的作用
碱性磷酸单酯酶的分离、纯化实验
10种必需氨基酸的作用
蛋白提取操作时的需知事项
细菌蛋白提取与纯化的实验手册
Western转印的优化
毕赤酵母表达实验手册
包涵体表达的蛋白的复性包涵体表达的蛋白的复性
蛋白激酶与磷酸酶在信号转导中的作用
蛋白透析中的沉淀问题分析和解决
蛋白质抽提实验方法大全
蛋白质为什么折叠——电脑程序告诉你
蛋白质分离纯化的一般程序
包含各种氨基酸的食物萝卜真营养
蛋白质提取与制备(Protein Extraction and Preparation)
利用沉淀实验测定蛋白质等电点
小鼠β
仪器校准周期从哪里开始计算？
《食品相关产品质量安全监督管理暂行办法》公布自2023年3月1日起施行
标准物质、标准样品使用的十个注意点
到了食品工厂，你应该看什么？
湿热灭菌的原理以及影响因素
多重疫情下实验室生物安全如何保证？
食品企业 "微生物“传播途径与控制？
【微生物染色】几种常用的微生物染色方法汇总！
如何成为一个优秀的QA?
实验室的压力表到底是要校准？还是检定？
标准溶液配制、赋值、管理的11条注意事项
检测人员的工作质量监督手册
化学反应中，氮气和氩气保护有何不同？
化学滴定法知识点，都给你整理在这了（附：标准滴定溶液的制备参考标准）
QA到底是干嘛的？
实验室人员能力验证的六个方法
你需要的实验室质量控制重点汇总解读!
pH计的维护保养及常见问题的解决方案！
正确使用标准物质的6个方面
检测人看过来，实验室管理规范知识汇总！
实验室溶液配制方法与技巧，基础中的基础！
QC经验总结50条
化学试剂药品取用原则，速看！
实验室耗材到底怎么管？
【指南】8招教你选购标准物质！
方法验证记录和报告的内容都有哪些？
必看！空白试验做不好，其它皆浮云
液相色谱不是堵就是漏，原因很简单！
验室常用的蒸馏水、去离子水、高纯水、超纯水的区别
分光光度计你真的会使用么
每个化验员都是“神枪手”！
化验室职责权限、工作要求及安全操作
你值得拥有的培训教程之仪器、设备管理
食品添加剂使用量该如何计算？
【审核】分享|实验室的内审和外审都有哪些区别？
【干货】实验室计量常见的10个问题
整理好你的化验室！
营养成分表检测，你应该知道的知识！
【菌种使用】菌液的制备！
盲、乱、散、松、空、错，质量记录过程中常见的问题你要学会解决
内卷不能输，有效数字运算规则知多少！
《化妆品抽样检验管理办法》公布！自3月1日起施行
原始记录填写应注意事项
检测人员必须知道的经典检测“三字经”!
实用丨实验室质量控制常用的10大方法，不会的赶紧学！
生物安全柜使用规范！
实验室现场评审需要准备哪些资料
试剂过期了怎么办？直接扔？
化学实验室经验分享：操作15“不”和22个“先后有别”
实验室安全的“四不伤害”
畜禽产品抽样技术和注意事项
掌握好理化实验五大质控要点，不加薪都难！
标准曲线查出样品是负值，如何报告结果？
骆驼奶粉中“塑化剂”超标，检测人怎么看？
日本排放核污水，涉及哪些产品哪些检测？
福林－酚法植物体内可溶性蛋白质含量的测定汇总
谷氨酸的作用
人血浆中缓激肽的SPE LC-MS/MS定量检测方法开发
iTRAQ技术——高通量蛋白质组学新利器
甲硫氨酸的作用
带你看清胶原蛋白的实力所在
酵母单杂交技术的原理、基本操作和用途
被科学家埋没了20年的绿色荧光蛋白
升级Western blot 同时检测大量蛋白方法
免疫球蛋白轻链的检测
单克隆抗体（McAb）和常规免疫血清抗体的特性比较
PALL公司Q hyperCel和S hyperCel应用实例
GST-pulldown检测已知原核蛋白A和真核过表达蛋白B相互作用操作流
真核生物高分子量DNA制备方法
蛋白质鉴定试剂双缩脲的特点及其与Fehling溶液的不同
丝氨酸的作用
蛋白分离措施详述
离子交换柱层析法分离可溶性蛋白的操作规范
重组蛋白溶解必读：PeproTech技术指南
融合蛋白表达系统常遇问题
经典Lowry–Folin法测定液体样品中蛋白质含量
MTT比色法检测影响细胞活力相关蛋白的操作步骤
膜蛋白[membrane protein]的分离
碱性磷酸酶的分离与纯化
植物叶蛋白的提取
原核表达（原理、材料与实验方案）
葡聚糖凝胶柱层析纯化PPO粗酶液
SDS-聚丙烯酰胺凝胶电泳试剂准备与操作步骤
福林酚试剂法测定蛋白质含量
PPO活性测定的原理方法与结果分析
植物蛋白质提取方法大全
植物组织蛋白质提取操作方法大全
Western blot 实验步骤及其注意事项
Science：首次利用新技术在天然条件下成功获得G蛋白偶联受体3D结构
蛋白质电泳－在蛋白质组学中的应用
专家点评：如何预防双向凝胶电泳中的水平条纹
大肠杆菌作为生物反应器获得特异蛋白需注意“N端法则”
常用试剂盒：BCA Protein Assay Kit
蛋白抽提指南（TRIZOL）
紫外分光光度法测定蛋白质含量
蛋白水解酶缘何溶解不了蜘蛛丝
LOWRY法和考马斯亮蓝法测定植物可溶性蛋白含量对比
质谱的试剂用品、实验操作、仪器和样品选择方法
你必须知道的质谱鉴定中14条FAQ
IPTG全面介绍：优点·使用·配制·特点
Western Blot杂交膜转移与免疫显色
纯水机的正确使用的注意事项！
如何解决平行样结果差异大？
碱度与PH值有什么关系？
答疑 | 如果标准是错的，实验室该怎么办？
如何处理实验室废弃物
我国食品安全国家标准体系简介
药品与仪器应该这样摆放
实验室废液要收集
除了“双人双锁”外，剧毒化学品还有“五双”管理
日本强排核污染水！海鲜海盐还能吃吗？日系化妆品还能买吗？
样品空白≠空白样品？样品空白常见疑问解答
加标回收试验做不好度原因是？
化验员日常情绪管理分类你中了几条！
实验室建设有哪些基本要求？这9点你都做对了么
突发！一大学实验室丢失剧毒氰化物，可致上百人死亡
实验室里的处事哲学
实验室比对是不是一定要找3家？
实验台之间安全距离是多少？
实验室环境保护管理程序
也许这份实验室急救指南你需要！
极端天气如何保障实验室安全？
安全生产月|如何做好自身的安全防护？
实验室事故应急指南！
炎炎夏日，实验室安全防范很重要！
高校实验室安全检查要点解读|规章制度
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化学试剂保存“八大防”
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实验室的发展趋势
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油田首个全国重点实验室来了！
投资超500亿，各地新晋“顶流”实验室你知道几个？
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绍芯实验室，宣布大消息！
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教育部发文，高校实验室安全分级分类管理
实验室安全管理的信息化与智能化发展趋势
工信部：围绕生物制造，再建设一批国家创新中心
川渝建筑工程质量检测不需要CMA了？
实验室经常被监督抽查的问题
检验检测机构常见问题和要求汇总
pH里的“p”为什么是小写？
检验检测实验室设计与建设技术要求第3部分：食品实验室
【检测】实验室各种日常记录，繁琐麻烦，如何规范？
你所了解的电波暗室实验室种类有多少？
常用的实验室分析仪器有哪些
实验室废液如何处理？
实验室的不符合项处理的标准流程，你了解多少？
实验室自动化，到底有没有价值？
实验室用品存放建议
实验室频发事故，主要源于哪些因素？如何避免？
一文了解实验室辐射安全知识！
实验室化学试剂存放要点！
划重点！实验室废液这样处理！
这些危化品相关知识你了解吗？
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仪器不接地的危害及预防方法
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消防安全之实验室篇
QC实验室最常见的77个整改不符合项！
气象灾害实验室网址大全
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细胞培养
明舟生物和细胞培养
明舟生物的细胞培养技术
什么是细胞培养技术
细胞培养技术对培养基的要求
什么是生物资源保藏中心
保藏中心是干什么的呢
保藏中心有什么任务
宁波明舟生物保藏中心怎么样
保藏中心都有哪些菌株
胞订购应该注意什么
中国科学家开发全新基因编辑工具，有望更安全应用于临床
国家微生物科学数据中心正式获批
细胞价格的影响因素
您觉得细胞需要多钱？
警示：3万余篇论文使用了身份错误的细胞或失去价值
第八期实验细胞培养及质量控制技术培训班
猿猴细胞病毒SV40转化的人脐静脉内皮细胞系
细胞培养基的基本知识
细胞培养基础问答 1
1.1细胞培养的基本原理与技术
体外培养的概念
细胞培养的一般过程
细胞培养的无菌环境
对成纤维细胞的生物学特性及成骨作用研讨
细胞培养的必备设施、常用器材和培养基
细胞在哪买才有保障
在哪里买细胞最安全
hel细胞株细胞系是什么？
你知道活细胞哪里有呢？
培养细胞的常规检查和观察方法
培养活细胞的观察方法
细胞固定观察法
细胞生长状况有关指标的检测方法
细胞固定观察法
细胞化学检测法
电镜观察法
The brain consumes half of a child's energy -- and that coul
Two new Earth-like planets discovered near Teegarden's Star
New methods from material sciences in physics find their way
Personalized medicine: Testing therapies on mini-tumors of h
Killing the unkillable cancer cells
The evolution of puppy dog eyes
Origin of life: A prebiotic route to DNA
The doctor who beat Ebola — and inspires other survivors to
Infants Choose Those Who Defer in Conflicts
Can deprescribing drugs linked to cognitive impairment actua
Scientists recreate blood-brain barrier defect outside the b
组氨酸的作用
蛋白质提取的方法总汇
植物组织蛋白质提取方法
蛋白质提取与纯化技术
SPA的提取、纯化与鉴定
免疫球蛋白提取技术：IgM的分离与提纯
免疫球蛋白提取技术：IgA的分离与提纯
免疫球蛋白提取技术：禽IgA提取与纯化
免疫球蛋白提取：连续提取免疫球蛋白
氨基酸的离子交换柱色谱分离
常用的标记免疫酶及其底物
一些提高蛋白质的稳定性的方法
NusA技术：显著增强各种“难溶蛋白”可溶性
蛋白浓缩基本方法
蛋白质的定量测定实验
蛋白质的浓缩技术
蛋白的生物活性测定方法
如何做原核表达
非常全面的各个蛋白数据库链接
Folin
酚试剂（Lowry）法测血清蛋白质含量
蛋白质的性质（一）蛋白质及氨基酸的呈色反应
Western Blot Protocol实验步骤
凝胶层析法蛋白质脱盐
酵母实验操作方案
原核表达之宿主菌株选择指南
考马斯蓝染色法原理
考马斯蓝染色法原理
考马斯亮蓝法测蛋白含量：药品的配制
Recreating embryonic conditions at break sites can help bone
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Cancer cells are quick-change artists adapting to their envi
细胞哪里便宜
细胞上哪买
收到细胞的处理方式
无菌操作基本技术
细胞传代培养
细胞计数与存活测试
细胞冷冻保存
细胞培养FAQ：
CELL LINE CELL TYPE SPECIES TISSUE MEDIUM
血清
第二章细胞培养液
第三章细胞培养的基本方法
第四章动物细胞大规模培养技术
第五章常见组织细胞的培养方法
第六章肿瘤细胞的培养
Researchers grow active mini-brain-networks
Translating proteins into music, and back
What made humans 'the fat primate'?
Could coffee be the secret to fighting obesity?
PC-3细胞与DU-145细胞的区别
DC2.4细胞
A549裸鼠移植瘤模型总结
裸鼠皮下成瘤常见问题解答
K562细胞诱导分化的研究进展
HL-60细胞与NB4细胞的区别
人血管内皮细胞模型PUMC-HUVEC-T1的应用情况
细胞怎么买
ATCC细胞购买后会有哪些问题
ATCC细胞是哪里生产的
实验设备使用规则
细胞培养注意事项
细胞培养USEFUL
Possible link between sugary drinks and cancer
Possible link between sugary drinks and cancer
Killing the seeds of cancer: A new finding shows potential i
How trees could save the climate
Widely available antibiotics could be used in the treatment
ATCC细胞是什么
ATCC细胞的种类
ATCC细胞的种类呢？
ATCC细胞发货形式有哪些
ATCC细胞的使用注意事项
ATCC细胞的培养条件
蛋白质定量实验方法大全
New cause of cell aging discovered
Brains work in sync during music therapy
Brain protein mutation from child with autism causes autism-
America's packaged food supply is ultra-processed
Vast majority of dietary supplements don't improve heart hea
Skin in balance: Joint forces of polarity and cell mechanics
Stem cell research sheds new light on the skin
购买细胞
建立细胞库的好方式都是什么？
购买原代细胞的注意事项？
购买293细胞的渠道是什么？
组建细胞所需要什么条件？
购买原代细胞的注意事项？
细胞购买价格受到什么因素影响
了解细胞系与细胞株的作用是什么？
细胞公司哪一个比较好？
上海细胞中心的实力如何？
细胞需要多少钱
细胞培养经验
体外培养的概念
无蛋白培养基与限定化学成分培养基
其他细胞培养用液
细胞分离技术
细胞的冻存与复苏
细胞计数及活力测定
细胞的分裂指数
细胞周期的测定
培养物的污染及防止
微载体培养技术（microcarrier culture technique）
上皮细胞培养
内皮细胞培养
神经细胞培养
肌组织细胞培养
巨噬细胞培养
肾小球分离、移植培养
肿瘤细胞的培养
肿瘤细胞的培养经验
ATCC细胞如何储藏
细胞要多少钱
293t细胞冻存细胞是什么？
lovo细胞株细胞系您了解多少呢？
nk细胞和t细胞的区别是什么
哪里买细胞最靠谱
简单介绍下人原代细胞
如何买细胞最正规
一、ATCC细胞发货形式有哪些
3.ATCC细胞的培养条件
4、ATCC细胞如何培养呢
5、保藏中心可以储存哪些细胞？
分子病理进展—细胞系身份认证
研究用细胞的选择
你还在使用MDA-MB-435D细胞吗？
ATCC菌株保藏方法
SRB菌株培养
TGB菌株培养
DH5a菌株
BL21(DE3) 菌株
干热灭菌
保藏中心的保藏方法有哪些？
菌株的传代-保藏-复壮
湿热灭菌
高压蒸汽灭菌
细胞/细胞系/ATCC细胞/细胞库/菌株
菌株 /质控菌株/对照菌株/ATCC菌株
保藏中心的冷冻保存法有哪些？
大肠杆菌
枯草芽孢杆菌
普通变形菌
产气肠杆菌
保藏中心有哪些
细胞有哪些
维氏气单胞菌菌种说明
链霉菌
葡枝根霉
KGN:人卵巢颗粒细胞
明舟生物细胞目录
明舟生物细胞目录1
黑曲霉
青霉
细胞可以用于肿瘤细胞治疗吗
简述细胞得多钱
简单分析一下细胞得多少钱
噬菌体效价实验
Ames氏致突变和致癌实验
费氏丙酸杆菌
人乳腺癌阿霉素耐药细胞株
粗糙脉孢菌顺序四分子分析
三孢布拉霉结合实验
鸡前脂肪细胞
人Ph+急淋白血病细胞
乙肝功能性治愈！
ATCC部分细胞简介
你的文章使用了正确的细胞吗？
人正常肝细胞（WRL68）
大鼠肺血管平滑肌细胞
大鼠视网膜微血管内皮细胞
如何选择合适的蛋白含量测定方法？
WESTERN BLOT PROTOCOLS
一种潜力无限的基因表达方法——甲醇酵母
毕式酵母表达常见的问题及其解析
大鼠脑胶质瘤细胞(C6)
人肺微血管内皮细胞
E.coli的GST-标记蛋白表达及纯化
肺大动脉内皮细胞
大肠杆菌蛋白表达纯化的策略
毕赤酵母蛋白不表达的原因
Invitrogen公司的Pichia酵母表达系统使用总结
原核表达实验的高招
小鼠骨髓间充质干细胞(mMSCs )
甲醇酵母表达实验的注意若干事项
噬菌体展示技术的原理及噬菌体展示系统
表达蛋白的生物学活性的检测操作步骤
大肠杆菌表达载体/穿梭载体/整合载体
Ecoli原核表达的实验原理/材料/实验方法
真核细胞常见的表达载体
葡聚糖凝胶柱的使用方法
凝胶过滤实验中的注意事项
亲和层析法纯化蛋白实验中需要注意的事项
His.Tag序列蛋白纯化法宝之HIS*BIND的选择
蛋白纯化反相柱的选择
蛋白质盐析分离法
蛋白信息传递功能之G蛋白在信息传递中的作用
蛋白凝血功能之凝血因子
蛋白的催化作用之淀粉酶
蛋白防御功能之免疫球蛋白
蛋白质识别功能作用之糖蛋白
蛋白储存功能之铁蛋白
蛋白转运功能之血红蛋白
蛋白质结构和功能的重难点
蛋白质结构与蛋白质折叠是蛋白生物学功能的基础
溶菌酶的相对分子质量测定方法
胶原蛋白的美容特性
胶原蛋白的分类
什么是胶原蛋白
胶原蛋白粉的的特点和使用方法
胶原蛋白多肽促进软骨组织的再生
酵母双杂交实验常见问题及其解决方法
酵母双杂交实验操作步骤大全
DEPC太危险了还是蛋白酶K吧
七种蛋白质互作实验方法总结
各种蛋白互作检测方法优缺点分析
胶体金标记蛋白A技术攻略
抗体标记及纯化服务
多克隆抗体制备服务
封闭剂小知识总结之封闭剂的种类及选择
BSA使用技巧讨论
BSA的配制方法
关于bsa标准曲线的制作方法讨论
关于BSA封闭液的使用方法大讨论
什么是生物染色剂印度墨水？
BSA在内切酶缓冲液中的作用
DNA分子的限制性内切酶消化操作流程
限制性内切酶的酶切实验原理与步骤
限制性内切酶分类指南
限制性内切酶市场 NEB厂商称雄
分光光度计维护方法说明
IPTG诱导pET载体目的蛋白表达的原因分析
12种非必需氨基酸的作用
LORRY法蛋白定量的实验步骤和需知事项
荧光淬灭法蛋白质定量实验方法与结果分析
蛋白质二维电泳技术原理简介
免疫共沉淀和免疫沉淀的图示和实验步骤
三种融合蛋白表达系统常见问题
二肽的概念与二肽合成的个人心得体会
纸层析法：分离鉴定氨基酸实验
蛋白不表达的原因知识汇总
Bradford法测蛋白质浓度——完整版
精氨酸的作用与副作用
在大肠杆菌中高效表达外源蛋白的全攻略
磷酸化蛋白的免疫印迹检测经验总结
检测蛋白磷酸化的六种方法及其优缺点
原核蛋白表达载体的讨论
心肌肌浆网钙-三磷酸腺苷酶的活性测定
蛋白质的等电点测定和沉淀反应
重组蛋白在E.coli中表达及纯化
豆粕中尿素酶活性的测定
BCA(二喹啉甲酸)蛋白分析法
核蛋白的提取方法
蛋白质组鉴定技术
蛋白质纯化的原理和方法（Protein Purification Principles and Methods）
Western Blot Analysis for Tissue
GST融合蛋白纯化--筛选表达株(英文）
western blotting的一些个人经验
WESTERN PROTOCOL
Western Blot Analysis by SDS
E.coli原核表达条件优化
常用蛋白质分子量标准参照物列表
NEB原核表达系统
羊多克隆抗体制备服务
SDS PAGE and Western Blotting
原核表达--宿主体系选择指南
Western blot and gel overlays（蛋白印记与凝胶覆盖）
蛋白质分离纯化
植物基因在大肠杆菌中的IPTG诱导表达
原核表达载体的重要调控元件
细胞膜蛋白质提取方法
融合蛋白的表达系统常见问题
GST融合蛋白表达与纯化的实验步骤与注意事项
酵母双杂交实验常见问题
凝胶层析法脱盐和分离蛋白质
亲和层析技术
醋酸纤维素薄膜电泳分离血清蛋白
金属螯合亲和层析分离蛋白质
蛋白浓缩方法详解
蛋白质与多肽提取分离
浅析蛋白纯化方法及其优缺点
超滤实验基本步骤
SPA的纯化
凝胶过滤注意事项
凝胶层析的优缺点
亲和层析过程中的注意事项
凝胶过滤的应用
6XHis 标签的特点以及它的使用方法
下游纯化工艺简介
纯化全长/高纯度蛋白方法（Double-tag）
包涵体的纯化
蛋白质纯化胶体过滤法
His-Tag重组蛋白亲和层析标准操作规程（SOP）
离子交换柱纯化蛋白的问题
关于柱层析的实验方法和技巧
超滤原理和优缺点
超滤类型及其原理
磁性微球在蛋白质和多肽的分离与纯化上的应用
蛋白质胶内酶解
如何从脂肪组织中提取总蛋白？
可溶性产物的纯化(融合T7·Tag的表达蛋白)
直击膜蛋白分析全过程——从表达，纯化到结构分析
蛋白质的提取和纯化--选择分离材料及预处理
蛋白质提取与制备的原理和方法
蛋白质抽取
提高超滤收率方法
蛋白质结构解析的方法简介
电泳切胶小窍门
蛋白质纯化离子交换法
蛋白质的提取及粗分离
离子交换层析
离心技术原理
蛋白质相互作用实验方法
无表面活性剂的膜蛋白提取方法
凝胶层析
凝胶过滤原理
葡聚糖凝胶柱使用及注意事项
NusA技术：显著增强大肠杆菌表达可溶、活性蛋白
亲和层析技术研究进展
亲和层析实验方法-溴化氰活化
亲和层析的应用
蛋白质纯化亲和层析法
亲和层析原理
选择纯化凝胶的具体方法
蛋白质纯化的一般原则及方法选择
蛋白质纯化武器—工艺篇
蛋白纯化实验经验总结
目标蛋白粗提方法
蛋白质纯化的方法选择
蛋白质纯化－人人都会用到的实用技术
人皮肤成纤维细胞（HSF细胞）
细胞渗透蛋白工程
大鼠肾上腺嗜铬细胞瘤细胞 PC-12(高分化)
核酸蛋白转移电泳及杂交
人肺癌细胞（Calu-1）
人非小细胞肺癌细胞(A549)
从酵母中大规模自然亲和纯化溶解膜蛋白
人肺腺癌细胞 (Calu-3)
人肺鳞癌细胞（NCI-H1703 ）
动物细胞或组织的蛋白质抽提步骤
人肺腺癌细胞(NCI-H1975)
现代生物分离技术在多肽蛋白质分离纯化中的应用
人肺鳞癌细胞（NCI-H226）
人肺腺癌细胞（NCI-H441）
重组蛋白纯化的基本策略
酪蛋白的提取
酶的提取、分离、纯化及其活力测定
人肺癌细胞(NCI-H292)
人肺癌细胞(PC9)
细菌中蛋白质的提取与纯化技术
人皮肤黑色素瘤细胞（SK-MEL-1）
垂直板聚丙烯酰胺凝胶电泳分离蛋白质
人正常肺上皮细胞(BEAS-2B)
人膜间皮细胞(MeT-5A)
蛋白质纯化之亲和层析法
人肺大动脉平滑肌细胞
蛋白质纯化之离子交换法
人肺大静脉内皮细胞
人食管癌细胞(Eca-109)
PPO活性测定
人食管癌细胞（TE-1）
人胃癌细胞（HGC-27）
蛋白质提取方法大全
人胃腺癌细胞(AGS)
人胃癌细胞(NCI-N87)
人胃腺癌细胞 (SGC-7901)
人结直肠腺癌细胞(Caco-2)
人结肠癌细胞(COLO 205)
人结肠腺癌细胞(COLO-320)
人结肠腺癌细胞(CW-2)
人结直肠腺癌细胞（COLO320 HSR）
人结直肠腺癌细胞（COLO320 DM）
人结肠癌细胞(HCT116)
人结直肠腺癌细胞(HCT-8)
人结直肠腺癌细胞(HCT-15)
人结肠癌细胞(HT-29)
人结直肠腺癌细胞(LoVo)
人十二脂肠腺癌 (HuTu-80)
人结直肠腺癌细胞（LS174T ）
人结肠直肠腺癌细胞（NCI-H508）
小鼠肠神经内分泌肿瘤细胞(STC-1)
人结肠腺癌细胞 (SW1116)
人结直肠腺癌细胞(SW-480)
人结肠癌细胞(SW620)
人肝癌细胞(BEL-7402)
人肝癌细胞(BEL-7405)
人高转移肝癌细胞(HCCLM3)
人肝癌细胞(Hep G2)
人肝癌细胞(HepG2.2.15)
人肝癌细胞(HuH-7)
人肝癌细胞(Huh7.5)
人肝星形细胞(LX-2)
人胚肾细胞（293A ）
高转移潜能肝癌细胞(MHCC-97H)
植物蛋白质提取方法总汇
蛋白纯化经验指南
GST－融合蛋白的表达与纯化
SDS-聚丙烯酰胺凝胶电泳对蛋白大小与纯度的鉴定
常用的层析分析方法
蛋白质沉淀法
蛋白质沉淀浓缩方法原理及详细解析
超滤技术的应用
多肽物质分离与分析方法研究进展
蛋白质浓缩提取和细胞裂解产品介绍
几种分子筛凝胶及应用
Column Buffers: CREB
Purification of TFIIA from E. coli
Procedure to purify protein from cells
Preparation of Dialysis tubing
Myosin Light Chain Preparation
Purification of TFIIIC
Preparation of Affinity Column制备亲和层析柱【UCSF】
Crystallization Trials
Preparation of Cell Lysate
Purification of recombinant sBRF M166L
Preparation of Cell Lysate
Purification of dnEBNA-1/Soft from E. coli BL21 LysS
Rapid Yeast Protein Prep for SDS PAGE and Western
Large scale nuclear extract preparat
Purifying Protein from Inclusion Bodies
Preparation of protein extracts for western blot
Urea Lysis Protocol
TCA protein precipitation
Yeast Nuclear Extract (Small Scale)
Purification of GST fusion proteins in E.coli GST融合蛋白纯化，方法二
Yeast protein prep for SDS PAGE and western (rapid)
Protein Expression and Purification of MBP-T antigen (131-259)
Purification of Calmodulin from Bovine Brain
离子交换层析介质的技术数据
Isolation of Protein from Tissue
Purification of Myosin Light Chain Kinase
Bacterial Protein Extraction (mini-scale)
Fusion Protein Isolation 融合蛋白分离纯化
Preparation of Tonsil Lysate
rat liver cytosol preperation
HIS-tagged protein purification
Yeast mini whole cell extract using glass beads for western analysis
Purification of Gcn4
rat liver cytosol preperation
离子交换纤维素层析法常用数据表及性质
Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
DEAE column: FPLC
Protocols for protein extraction from whole tissues for isoelectric focusing
低转移人肝癌细胞(MHCC97-L)
人肝癌细胞(SMMC-7721)
人肾细胞腺癌细胞(769-P)
人肾透明细胞腺癌细胞(786-O)
人肾癌细胞(A498)
人肾癌细胞(A-704)
人肾细胞腺癌细胞(ACHN)
人肾皮质近曲小管上皮细胞（HK-2)
人肾癌细胞(OS-RC-2)
人膀胱癌细胞（HT-1376 ）
人前列腺癌细胞(LNCAP)
人膀胱癌细胞（EJ）
人膀胱移行细胞癌细胞(J82)
人膀胱移行细胞乳头瘤细胞(RT4)
人膀胱移行细胞癌细胞(T24)
人正常膀胱上皮细胞(SV-HUC-1)
人膀胱移行细胞癌细(UM-UC-3)
人前列腺癌细胞(DU145 )
人眼脉络黑色瘤细胞（C918）
人高转移鼻咽癌细胞（5-8F ）
人胰腺癌细胞(PC3)
人前列腺癌细胞(VCaP)
人正常前列腺基质永生化细胞(WPMY-1)
人眼小梁网细胞(HTMC)
人喉癌细胞（Tu212）
人喉癌细胞（TU 686）
人胰腺癌细胞（Capan-2 ）
人甲状腺癌细胞(B-CPAP)
人甲状腺鳞癌细胞(SW579)
人髓样甲状腺肿瘤细胞（TT）
人胰腺癌细胞(Capan-1)
人胰腺癌细胞（JF-305 ）
人胰腺癌细胞（MIA-PACA-2 ）
人胰腺癌细胞（PANC-1）
人胰腺癌细胞（PATU 8988t）
人胰腺癌细胞(HPAC)
人胰腺癌细胞（PANC 05.04 ）
人胰腺导管腺癌细胞（PL45 ）
人胰腺癌细胞(SW 1990)
人原位胰腺腺癌细胞(BxPC-3)
人卵巢腺癌细胞(Caov-3)
人卵巢癌细胞(SK-OV-3)
人卵巢癌细胞胞(CoC1)
人卵巢透明细胞癌(ES-2)
人子宫颈鳞癌细胞(SiHa)
人卵巢癌细胞(OVCAR-3)

MAPK signaling pathway - Homo sapiens (human)

细胞坏死(necrosis)

Basic Method for Direct Immunofluorescence Labeling

原代软骨细胞分离培养的总结

肿瘤细胞的培养

细胞培养基中常用氨基酸及其作用

溶酶体（lysosome）

人粒细胞-巨噬细胞集落刺激因子（GM-CSF）酶联免疫分析

血小板活化检测方法

电子显微镜的使用与细胞超微结构观察

lipofectamine 2000脂质体转染293ft细胞经验

果蝇的外部形态观察及饲养技术

转染实验需知

最全面的MTT相关技巧大汇总

细胞培养常见26个问题

单克隆抗体技术(monoclonal antibody technique)

如何选用特殊细胞系培养基？

细胞生长检测4：MTS检测法

Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination

Cyclic AMP- Dependent Protein Kinase Activity Ratio Assay

Coimmunoprecipitation Assay

Sperm Morphology Assessment Using Strict (Tygerberg) Criteria

The Perinucleolar Compartment (PNC): Detection by Immunohistochemistry

细胞生物基本方法：肿瘤细胞培养

大鼠III型胶原蛋白（COL-III）酶联免疫分析（ELISA）

同源异型突变(homeotic mutation)

植物原生质体的制备

DEAE-葡聚糖介导的转染步骤

TISSUE CULTURE ON COVERSLIPS

胰岛素受体(insulin receptor)

无血清培养基

Nature子刊上的akt pathway的信号通路图

人成纤维细胞生长因子23（FGF-23）酶联免疫分析

Freezing and Thawing of MEFs

Protocol for Aortic Ring Assay

吞饮作用(pinocytosis)

核糖体（ribosome）

酵母遗传学方法6：羧肽酶Y的平板鉴定

流式细胞仪之细胞凋亡检测的PI双染色法

Use of EIA to Measure MMPs and TIMPs

Apoptosis Induction

激素(hormone)

Meiotic Cytogenetics in Coprinus cinereus

Scaffolds for Tissue Engineering and 3D Cell Culture

PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION (2 METHODS)

AKT信号通路概述

新的高内涵成像系统acumen® hci隆重上市

无水氯化镁黑色液体的特性及应用解析

细胞核(nucleus)

Studying Amino Acid Transport Using Liposomes

从G蛋白偶联受体到MAPK的通路图

Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes

Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Prot

The Production of Monoclonal Antibodies to Membrane Proteins

Use of Liposomes to Study Cellular Osmosensors

质体转染293细胞后细胞漂浮解答

姐妹染色单体分化染色方法

人中性粒细胞弹性蛋白酶（NE）酶联免疫分析（ELISA）

细胞凋亡形态学检测与观察

Preparing chemically competent cells

Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Location

Identification of Target Genes of a Yeast Transcriptional Repressor

Analysis of Skeletal Muscle Development in Drosophila

Studies of Adult Neural Stem Cell Migration

Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays

胸腹水染色体标本制备

Approaches to Cloning of Pain-Related Ion Channel Genes

ES Cell-Mediated Conditional Transgenesis

细胞培养的微生物污染排除

Imaging Lipid Bodies Within Leukocytes with Different Light Microscopy Techniques

小白鼠染色体的制备和观察

交换

Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors

RNA Extraction From Drosophila Heads

Linearization and Purification of the Shuttle Plasmid

Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extract in Semi-Intact MDCK Cells

内膜系统（endomembrane system）

Detection of Tight Junction Barrier Function In Vivo by Biotin

Single Cell Analysis of Lipid Rafts

Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes