所有文章_生物资料网

汽水
最大持续产量（maximum sustained yield）
水的生物净化
蛋白质生物合成的原料
晶格
类别异构
武汉专家利用油菜籽渣炼出生物柴油
辅酶（coenzyme）
巴西呼吁进行非洲生物燃料“革命”
果糖（fructose）
自主序列复制系统
野生大麦(wild barley)
转录依赖的扩增系统
猪生长激素（GH）酶联免疫分析
猪干扰素-γ（IFN-γ）酶联免疫分析（ELISA）
猪传染性胸膜肺炎 (PCP)酶联免疫分析（ELISA）
猪钙调神经磷酸酶(CaN)酶联免疫分析
猪表皮生长因子(EGF)酶联免疫分析（ELISA）
重水
中子
白垩纪（Cretaceous Period，Cretaceous）
赣将建国内首个生物柴油用于车辆行驶项目
海棠脯
质子
香水梨干
鳗鱼火腿
酒心巧克力
红烧鲐鱼
茄汁沙丁鱼罐头
凤尾鱼罐头
茄汁黄鱼
精制巧克力
糖醋瓜缨
进化速度（speed of evolution）
生物元素
植物吲哚乙酸（IAA）酶联免疫分析（ELISA）
层片（synusia）
植物脱落酸(ABA)酶联免疫分析（ELISA）
植物钙调磷酸酶（CaN）酶联免疫分析（ELISA）
植物超氧化物歧化酶（SOD）酶联免疫分析（ELISA）
菠萝果冻
糖桔饼
猕猴桃酸奶
人体气味的色谱分析
蛋白复性（三）-蛋白质的结构转换
RNA操作中小结
海洋牧业—一蓝色革命
臭氧层空洞
离子的极化
鲜味果酱
气体摩尔体积
光谱分析——原子吸收分光光度法
玉米秸饴糖
杏仁牛奶冰霜
桔味棒冰
胡桃冰砖
栗子冰砖
玫瑰杨梅
菠萝酱
山楂饼
仙桃酱
黑加仑子果酱
槟子酱
麻酥糖(上海)
鱼松
鱼松(江南风味)
咸味杨梅
果香醉枣
杨梅干(苏式)
家制桃脯
牛奶苹果泥酥
香草话梅(苏式)
玫瑰蜜饯(川式)
梨子蜜饯(川式)
桂花蜜饯(川式)
去皮酥(福式蜜饯)
蜜橙皮(川式)
西瓜冻
西瓜皮糖酱
香草桃片(广式)
甘草酸枣
糖柿片
腌沙丁鱼
家制山楂糕
干蜜饯樱桃
木瓜脯
栗子糕
橙皮脯
南味花生糖
白糖胡桃
家制苹果酱
广饼(川式蜜饯)
无核蜜枣
桂花橄榄(福式)
花生牛轧糖
开水米花糖
牛奶红果泥
萝卜糖(川式)
山楂薄片
稀土元素
水葫芦(common water hyacinth)
鱼酚氧化酶(PO)酶联免疫分析(ELISA)
鱼唇
栽培稻(cultivated rice)
金属
金属晶体
极性键
鲜红薯制麦芽糖
盆栽果树（fruit culture in pot）
黄芪大枣汤
桐乡循环经济让废物实现“再生”
FISH技术在白血病中的应用
樱桃果汁
延胡索(yanhusuo)
异硫氰酸胍法
胰酶消化修复抗原
电价规则
灵芝黄芪酒
中式冷点
血糖的斐林法测定
种子蛋白质系统分析:种子蛋白质含量测定（凯氏定氮法）
酶切常见问题总结
液化气
分子生物学试验方法探讨一－Northern blots 技术
鸭孕激素/孕酮（PROG）酶联免疫分析（ELISA）
血红素结合蛋白（hemopexin,Hpx）
雪耳白果糖水
布朗运动
玄参(ningpo figwort)
秀丽线虫RNA提取--C. elegans RNA prep
新疆绿葡萄干
英国一家公司用巧克力废料生产生物燃料
葡萄缺锌症
小鱿鱼片
小鼠组织因子(TF)酶联免疫分析
小鼠乙酰胆碱转移酶（ChAT）酶联免疫分析（ELISA）
小鼠维生素D3酶联免疫分析（ELISA）
小鼠透明质酸(HA)酶联免疫分析（ELISA）
小鼠去甲肾上腺素（NA）酶联免疫分析（ELISA）
小鼠前列腺素 E2(PGE2)酶联免疫分析（ELISA）
小鼠凝血因子ⅩⅢ（ⅩⅢ)酶联免疫分析（ELISA）
小鼠淋巴球性脉络丛脑膜炎病毒（LCM）酶联免疫分析
小鼠抵抗素样分子β (RELM-β)酶联免疫分析（ELISA）
小鼠的组织学技术--组织处理，染色
小鼠胆红素(TB)酶联免疫分析（ELISA）
小鼠促肾上腺皮质激素(ACTH)酶联免疫分析（ELISA）
小鼠促卵泡激素（FSH）酶联免疫分析（ELISA）
小鼠M-胆碱能受体结合力(MCBC)酶联免疫分析（ELISA）
小鼠一氧化氮（NO）酶联免疫分析
小鼠血管内皮生长因子（VEGF)酶联免疫分析（ELISA）
小鼠三磷酸肌醇(IP3)酶联免疫分析（ELISA）
小鼠前列环素(PGI2)酶联免疫分析（ELISA）
小鼠脑啡肽(ENK)酶联免疫分析（ELISA）
小鼠免疫球蛋白M(IgM)酶联免疫分析（ELISA）
小鼠Ⅵ型胶原（Col Ⅵ）酶联免疫分析（ELISA）
小鼠8异前列腺素F2α(8-iso-PGF2α)酶联免疫分析
小鼠尿微量白蛋白(ALB）酶联免疫分析（ELISA）
蛋白质谱分析方法特点及其在蛋白组学研究中的应用
大肠杆菌三种DNA聚合酶的性质比较
PCR技术：Taq DNA聚合酶属性
三种DNA聚合酶的作用特点和主要功能简述
气相色谱操作中的注意事项
气相色谱柱的安装操作流程
液相色谱泄漏处理方法
如何延长色谱柱的寿命
色谱仪操作规程之BECKMAN 125型高效液相色谱仪
色谱仪操作规程之P230高效液相色谱仪系统
气相色谱层析技术条件选择、操作方法与结果分析
非同位素原位杂交组化与免疫组化联合法实验步骤
几种特殊薄层层析及应用
NBS（N-溴代丁二酰亚胺）在有机反应中的应用研究进展
中国第一台纳米离子探针的原理和分析方法
不连续SDS
旋光法测定旋光物质含量
核酸的定量测定:紫外吸收法
高效液相色谱仪常规分析操作步骤
国内部分生物芯片公司介绍
DNA: DNA in situ hybridization
吸附柱色谱的实验技术
维生素C的定量测定及碱、热、铜离子对其的破坏作用
血红蛋白凝胶柱层析
双向免疫扩散试验
DNA
液相色谱常见问题及处理办法
2
3% TREVIGEL PROTOCOL for 3 bp resolution
全自动电泳疑问解答FAQs
Agarose gel electrophoresis（琼脂糖凝胶电泳）
五种分子生物学常用贮存液的配制方法
芯片
蛋白质芯片
让芯片“吞食”人类？
琼脂糖凝胶电泳技术
线虫基因组杂交-Worm genomic Southern blots
水花生(slligator alternenthera)
虾酚氧化酶(PO)酶联免疫分析(ELISA)
DNA-chip technologies(DNA芯片技术)
稀土微肥
西瓜子的加工
五香烤鱼
三七(sanchi)
西洋参(American ginseng)
无花果酱
深圳市成品油的行业发展规划出台
凝胶回收常见问题分析
缺疗与氧中毒
急性期反应蛋白
原子结构和分子结构--　原子结构
应激查机体的机能变化
呼吸衰竭的防治原则
防治缺血／再灌注损伤的展望
醛、酮、醌--　醛和酮
应激时的物质代谢变化
呼吸衰竭时机体的主要机能代谢变化
热休克蛋白
DNA重组与基因工程--　工具酶
糖类--　单糖
氧代酸
休克的原因和分类
烯烃
急性肾功能衰竭
基因治疗
电极电位
混合型酸碱平衡障碍
心力衰竭的发病机制
碱中毒
链烃--　烷烃
PH值与缓冲溶液--　水的离子积和溶液的PH值
酸中毒
醚
二烯烃
水肿--　概述
平衡失调的检测指标
黄疸
[附]：高血压
分子生物学实验基础知识简介
脱水菠萝
兔子CD147酶联免疫分析（ELISA）
兔子Ⅱ型胶原(Col Ⅱ)酶联免疫分析（ELISA）
兔肿瘤坏死因子α（TNF-α）酶联免疫分析（ELISA）
兔CD34分子(CD34)酶联免疫分析
兔骨钙素(BGP)酶联免疫分析（ELISA）
天蚕(Japanese tussah silkworm)
栗蚕(camphor silkworm)
透析袋截留分子量选择参照表（各常见物质分子
糯米苕丝糖
桃制果丹皮
蒲桃(rose-apple)
太阳
多味木瓜脯
生物能源：耕种“后石油时代”
水糖
杨柳科(Salicaceae)
柿饼
无花果干
十一金属螯合亲和层析分离蛋白质
糖的性质测定实验方法步骤
鱼糜挂面
太子参(heterophylla falsestarwort)
石蜡切片 Parrafin Sectioning
初级生产量
生化与分子生物学技术在生命医学研究中的运用
气候变化
集雨节灌工程
山茱萸(common macrocarpium)
沙漠生态系统
败血病(septicemia)
乳糖
江苏洁美把“地沟油”炼成生物柴油
人乙酰胆碱转移酶（ChAT）酶联免疫分析（ELISA）
人乙酰胆碱受体抗体(AChR-Ab)酶联免疫分析（ELISA）
人衣原体（Chlamydia）酶联免疫分析（ELISA）
人新喋呤（Neopterin）酶联免疫分析（ELISA）
人心果( sapodilla)
人纤溶酶原激活物抑制因子 (PAI)酶联免疫分析
人脱氢表雄酮硫酸酯(DHEA-S)酶联免疫分析（ELISA）
人铁调素Hepcidin酶联免疫分析（ELISA）
人瘦素（LEP）酶联免疫分析（ELISA）
人溶血磷脂酸(LPA)酶联免疫分析（ELISA）
人醛固酮（ALD）酶联免疫分析（ELISA）
人前铁调素（Pro-Hepc)酶联免疫分析（ELISA）
人前列腺素Ｅ２（PGE2）酶联免疫分析
人凝血酶（Thrombin）酶联免疫分析（ELISA）
人卵磷脂胆固醇酰基转移酶（LCAT）酶联免疫分析（ELISA）
热污染（thermal pollution）
人酪氨酸激酶（TKs）酶联免疫分析（ELISA）
人可溶性CD30(sCD30)酶联免疫分析（ELISA）
人抗磷脂酰胆碱抗体IgG(APC-IgG)酶联免疫分析(ELISA)
人激肽释放酶6(KLK-6)酶联免疫分析（ELISA）
人激肽释放酶10（KLK 10）酶联免疫分析（ELISA）
人骨钙素(BGP)酶联免疫分析（ELISA）
人谷胱甘肽-过氧化物酶（GSH-PX）酶联免疫分析
人的幽门螺杆菌IgM（HP IgM）酶联免疫分析（ELISA）
人不对称二甲基精氨酸(ADMA)酶联免疫分析
人表皮生长因子受体2（Her2）酶联免疫分析（ELISA）
人Toll样受体(TLR)酶联免疫分析
人CYP2D6酶联免疫分析
人组织转谷氨酰胺酶(tTG)酶联免疫分析
人组织因子途径抑制物(TFPI)酶联免疫分析(ELISA)
人重组人表皮生长因子（rh-EGF）酶联免疫分析（ELISA）
人脂蛋白去乙酰化酶D3酶联免疫分析
人游离脂肪酸（FFA)酶联免疫分析
人分泌型磷脂酶A2（sPLA2)酶联免疫分析（ELISA）
人肺部活化调节趋化因子(PARC/CCL18)酶联免疫分析
人对氧磷酶1(PON1)酶联免疫分析
人超氧化物歧化酶（SOD）酶联免疫分析
人β－内啡肽（β－EP）酶联免疫分析（ELISA）
人NADPH氧化酶（NADPH-OX）酶联免疫分析(ELISA)
人CD147酶联免疫分析（ELISA）
人apelin 12(AP12)酶联免疫分析（ELISA）
人25羟基维生素D(25-OH-D)酶联免疫分析（ELISA）
人一氧化氮合成酶(T－NOS)酶联免疫分析（ELISA）
人血管内皮生长因子D(VEGF-D)酶联免疫分析（ELISA）
人血管紧张素Ⅱ（AngⅡ)酶联免疫分析（ELISA）
人沙眼衣原体(CT)酶联免疫分析（ELISA）
人乳腺癌标志物-CA153酶联免疫分析（ELISA）
人内皮型一氧化氮合成酶(eNOS）酶联免疫分析（ELISA）
人可替丁(Cotinine)酶联免疫分析（ELISA）
人降钙素基因相关肽(CGRP)酶联免疫分析（ELISA）
人脊髓灰质炎IgG抗体（PV-IgG）酶联免疫分析（ELISA）
人环磷酸鸟苷(cGMP)酶联免疫分析(ELISA)
人Toll样受体6(TLR6)酶联免疫分析
人I型胶原（COL-I）酶联免疫分析（ELISA）
人HBV PreS2酶联免疫分析（ELISA）
人HBV PreS1酶联免疫分析（ELISA）
犬肿瘤坏死因子α（TNF-α）酶联免疫分析（ELISA）
犬瘦素（leptin）酶联免疫分析
犬醛固酮(ALD)酶联免疫分析
犬凝血因子Ⅶ（FⅦ）酶联免疫分析（ELISA）
犬促卵泡激素（FSH）酶联免疫分析（ELISA）
犬传染性肝炎病毒抗体(ICHV-Ab)酶联免疫分析（ELISA）
犬白三烯D4(LTD4)酶联免疫分析（ELISA）
犬白三烯B4(LTB4)酶联免疫分析（ELISA）
犬(T)酶联免疫分析（ELISA）
犬心钠素(ANP)酶联免疫分析（ELISA）
犬绒毛膜促性腺激素(HCG)酶联免疫分析
犬前列腺素（PG）酶联免疫分析
犬内皮素(ET)酶联免疫分析
犬环磷酸腺苷(cAMP)酶联免疫分析
犬二胺氧化酶(DAO)酶联免疫分析（ELISA）
犬促性腺激素释放激素(GnRH)酶联免疫分析
犬白三烯C4（LTC4）酶联免疫分析（ELISA）
犬D-乳酸(D-Lactate)酶联免疫分析
犬诱导型一氧化合成酶（iNOS）酶联免疫分析（ELISA）
犬一氧化氮（NO）酶联免疫分析
琼脂糖凝胶的浓度与DNA分离范围
氢化物
轻金属
山楂(hawthorn)
芹菜
猪流感
葡萄缺硼症
膨化谷物
蔬菜缺硼症
聚丙烯酰胺凝胶电泳凝胶的配制
牛醛固酮(ALD)酶联免疫分析
牛口蹄疫抗体酶联免疫分析（ELISA）
牛单链DNA(ssDNA)酶联免疫分析（ELISA）
牛促卵泡素（FSH）酶联免疫分析（ELISA）
凝胶层析技术
镍镉电池
有效碰撞
稀溶液的依数性
紫花苜蓿(alfalfa)
果胶软糖
牡蛎干
适应度（fitness）
摩丝
摩尔
主量子数
免疫PCR
蜜青梅(苏式)
美国参议院金融委员会主席概述24亿美元能源税收计划
Cell：人类三分之一基因由小RNA调控
酶法、热修复、微波法修复抗原
城市煤气
麻松条
人工林
LCR
藜谷(quinoa)
冷烫精
天麻(tall gastrodia)
辣椒
昆虫乳酸脱氢酶（LDH）酶联免疫分析
半导体芯片的兄弟——生物芯片
开胃芒果
铁观音(tieguanyin)
菲律宾全岛种植“柴油树”
菲律宾72.53万公顷专门种植生物燃料农作物
法国2008年油菜籽播种面积预计减少10%
菊芋（Jerusalem artichoke）
软饮料soft drink
精子顶体染色 Stain for Sperm Acrosomes
废弃食用油炼柴油泰国警察缓解油荒
NEB　T4连接酶使用说明
浇切糖
姜糖
碱变性法、煮沸法、碘化钠法提取制备模板核酸
甲胎蛋白（α-fetoprotein）
家蚕羧肽酶（CP）酶联免疫分析（ELISA）
柠檬瓜条
基因芯片在临床上的应用
地高辛配基随机标记DNA探针法
鸡腿菇
鸡卵黄免疫球蛋白（IgY）酶联免疫分析（ELISA）
黄螺干
化学试剂的包装一般原则
式量
花生糖
花生酥糖
花色巧克力糖
护发化妆品
猴肿瘤坏死因子α（TNF-α）酶联免疫分析（ELISA）
猴肾素（Renin）酶联免疫分析（ELISA）
猴降钙素(CT)酶联免疫分析
等离子态
芝麻酥糖
合成气
拟高粱
胡萝卜汁
海水生物群落
国内首个生物酶法生物柴油项目动工
造血干细胞自我更新调控及wnt信号在其中的作用
中华医学会北京分会医学病毒学会会议纪要
不同人群血清抗GBV-B的检测及其意义
用微型形状记忆合金骑缝钉治疗牙隐裂的理论及实验研究
双歧杆菌粘附素的提纯
室温对抗丙型肝炎病毒抗体检测ELISA试验的影响
副溶血性弧菌直接溶血毒素基因原核表达载体的构建
生物电阻抗断层成像技术的研究进展
新型病毒TTV感染的初步探讨
丙型肝炎病毒相关肽抗体的测定
丙型肝炎肝外组织内病毒抗原的存在及意义
RNA干涉在基因治疗中的应用
绝灭（extinction）
糖水苹果
糖水籽瓜瓤
糖浆苹果
无核加应子
糖水桔片
苹果脯
无核糖枣
糖浆广柑
乌梅干
糖浆金桔
杏干
糖水金桔
糖水梨
桃果泥
李干
糖水樱桃
黑枣
糖水桃子
西瓜汁
蜜饯西瓜皮
杏酱
猕猴桃干
大枣罐头
糖西瓜条
播种(sowing)
常用缓冲液之各种pH值的Tris缓冲液的配制
各型休克的特点
高温处理＋柠檬酸钠或EDTA修复石蜡包埋样品抗原
佛手梅(苏式)
蜂蜜麻糖
维生素分类
乳中非蛋白氮的测定方法
葡萄白粉虱
多座位同工酶
薇菜(vetch)
苏州丁果糖
电子
酸碱平衡紊乱--　正常平衡的调节
第四纪（Quaternary Period）
二叠纪（PermianPeriod）
地高辛(Digoxin)酶联免疫分析（ELISA）
鱼粉
巴西Petrobras预计08年将仅出口5亿万升乙醇
原子核外电子排布
蛋白质组
蛋白质化学(Chemistry of Protein)
蛋白酶K消化裂解法提取制备模板核酸
大鼠血管舒缓激肽（BK）酶联免疫分析
大鼠胃蛋白酶(Pepsin)酶联免疫分析
大鼠丝裂原激活的蛋白激酶/MAP激酶(MAPK)酶联免疫分析
大鼠瘦素（leptin）酶联免疫分析
大鼠热休克蛋白-72（HSP-72）酶联免疫分析（ELISA）
大鼠去甲肾上腺素（NA）酶联免疫分析
大鼠酒精性脂肪肝（AFL）酶联免疫分析
大鼠胱硫醚-r-裂解酶（CSE）酶联免疫分析（ELISA）
大鼠谷胱甘肽转移酶(GST)酶联免疫分析（ELISA）
大鼠二酰基甘油(DAG)酶联免疫分析
大鼠二氨氧化酶（DAO）酶联免疫分析（ELISA）
大鼠CD8分子（CD8）酶联免疫分析（ELISA）
大鼠17β-羟基类固醇脱氢酶Ⅲ（17βHSDⅢ）酶联免疫分析
大鼠组胺（HIS）酶联免疫分析
大鼠游离脂肪酸（FFA)酶联免疫分析
大鼠乙酰胆碱 (ACh)酶联免疫分析（ELISA）
大鼠总补体（CH50）酶联免疫分析(ELISA)
大鼠一氧化氮合成酶(T－NOS)酶联免疫分析（ELISA）
大鼠心肌肌钙蛋白Ⅰ(cTn-Ⅰ)酶联免疫分析（ELISA）
大鼠三碘甲状腺原氨酸(T3)酶联免疫分析
大鼠羟脯氨酸(Hyp)酶联免疫分析（ELISA）
大鼠抗酒石酸酸性磷酸酶 (TRAP)酶联免疫分析（ELISA）
大鼠谷胱甘肽还原酶（GR）酶联免疫分析(ELISA)
大气污染
菠萝糖
杨桃糖
甘草杨桃
酵母蛋白质的抽提
硝酸盐培养基
缓冲葡萄糖蛋白胨水(MR和VP试验用)
5g/L淀粉指示剂如何配制
糖分析
鞍形花蟹蛛(saddle crab spider)
菠萝脯
菠萝果丹皮
冰糖葫芦
直接裂解法提取制备模板核酸
中国限制外商投资生物燃料
姜汁李
华勃氏呼吸计法测定L—谷氨酸
乳过氧化物酶法（LPO）
粮价上涨引发通胀生物能源是把双刃剑
脂多糖预处理和己酮可可碱对大鼠急性肺损伤的
簿层层析
大气
半乳糖苷酶和碱性磷酸酶染色方法Histochemical Staining
半金属
浙贝母(thunberg fritillary)
白蛋白（albumin）
巴西将实施全国生物柴油计划
铵态氮肥
桉叶药糖
Measurement of -Carotene15,15-Dioxygenase Activity by Reverse-Phase HPLC
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Live Cell Imaging of Zebrafish Leukocytes
Polyclonal Antibody Production
Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Electrophoresis
YAC Library Screening: Hybridization and PCR-Based Screening Protocols
Synthesis of Biodegradable Polymers Using Biocatalysis with Yarrowia lipolytica Lipase
X-Ray Microanalysis in the Scanning Electron Microscope
Crystallographic Studies of Antigen-Antibody Interactions
X-Ray Crystallography
X-Ray Absorption Spectroscopy
无机物相关
人体结构
The Deposition of a Biocide in Wood-Based Material
Compartmentalizing Genetically Encoded Calcium Sensors
Aromatic Hydroxylation: Salicylic Acid as a Probe for Measuring Hydroxyl Radical Production
The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chroni
Cryoelectron Microscopy of Icosahedral Virus Particles
Solid-Phase Guanidinylation of Peptidyl Amines Compatible with Standard Fmoc-Chemistry: Formation of
Synthesis of Coumarin-Based, Esterase-Sensitive Cyclic Prodrugs of Opioid Peptides with Enhanced Mem
Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides
PCR-Based Construction of Long Hammerhead Ribozymes
Database Similarity Searches
Using Quantitative Fluorescence Microscopy to Probe Organelle Assembly and Membrane Trafficking
Lipase Activation and Stabilization in Room-Temperature Ionic Liquids
Nitric Oxide Releasing Nanoparticle Synthesis and Characterization
Enzyme Stabilization and Immobilization by Sol-Gel Entrapment
Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue
Follow-Up of Natural Product Isolation
Assessment of Cyclooxygenase Protein Expression by Western Blotting
Coupling Peptides To Resin For Affinity Purification
The Geisler Method: Tracing Activity-Dependent cGMP Plasticity Changes upon Double Detection of mRNA
Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans
Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect stimu
Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli
Microwave-Assisted Method for Fabrication of Carbohydrate Cluster Microarrays on 3-Dimensional Hydra
Deoxyribozyme-Based, Semisynthetic Access to Stable Peptidyl-tRNAs Exemplified by tRNAVal Carrying a
Computer Simulation of MAPK Signal Transduction
Modulation of human interferon- biosynthesis by antisense oligodeoxynucleotides
Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library
A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1
A method for detecting functional activity related expression in gross brain regions, specific brain
Analyzing ligation mixtures using a PCR based method
The Synthesis of Bicyclic Piperazinone and Related Derivatives
Ligand Binding to Oligonucleotides
Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes
Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds
Force and compliance measurements on living cells using atomic force microscopy (AFM)
Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors
Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction
An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carri
An antibody-lectin sandwich assay for quantifying protein glycoforms
Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Int
Systems for the Cell-Free Synthesis of Proteins
Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the sa
Quantitative Proteomics of Mouse Brain and Specific Protein-Interaction Studies Using Stable Isotope
Protein Processing Characterized by a Gel-Free Proteomics Approach
A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts
Quantification of Proteins and Metabolites by Mass Spectrometry Without Isotopic Labeling
In Vitro O-Antigen Ligase Assay
Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterol
Negative staining and image classification powerful tools in modern electron microscopy
Use of Bimolecular Fluorescence Complementation in Yeast Saccharomyces cerevisiae
Chemokine Receptor Interactions with Virus-Like Particles
Studying the ArfGAP-Dependent Conformational Changes in SNAREs
Polyclonal Antibody Production 简单快捷制备多抗方法，可用于western、ELISA和免疫/b
The Preparation of Phenyl-stilbene Derivatives Using the Safety Catch Linker
Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy
In Vitro Vascularization of Human Connective Microtissues
The Molecular Biology of Furin-Like Proprotein Convertases in Vascular Remodelling
Immunoaffinity, Capillary Electrophoresis, and Statistics for Studying Intact Alpha 1-Acid Glycoprot
Spectral Analyses of Cytochromes P450
Enzymes Acting on d-Amino Acid Containing Peptides
Large-Scale Isolation of Ti Plasmid DNA
Urine Sample Preparation and Protein Profiling by Two-Dimensional Electrophoresis and Matrix-Assiste
Determination of Uric Acid in Urine by Fast-Scan Voltammetry (FSV) Using a Highly Activated Carbon F
Formation of Apolipoprotein AI-AII Heterodimers by Oxidation of High-Density Lipoprotein
Anticonvulsants
Asymmetric Syntheses of Unnatural Amino Acids and Hydroxyethylene Peptide Isosteres
Incorporation of Unnatural Non--Amino Acids into the N-Terminus of Proteins in a Cell-Free Translati
Chemical Cleavage (Maxam and Gilbert) Method for DNA Sequence Determination
DOP-PCR Amplification of Whole Genomic DNA and Microchip-Based Capillary Electrophoresis
Chemogenomic Analysis of Safety Profiling Data
Multiple Embryo Time-Lapse Imaging of Zebrafish Development
Improved Cellular Uptake of Antisense Peptide Nucleic Acids by Conjugation to a Cell-Penetrating Pep
Optimization of naked DNA delivery for interferon subtype immunotherapy in cytomegalovirus infection
Bacterial Genome Mapping by Two-Dimensional Pulsed-Field Gel Electrophoresis (2D-PFGE)
Eukaryotic Cell Labeling and Preparation for 2-D
Computational Methods for Analysis of Two-Dimensional Gels
Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-
Cryopreservation of Mammalian Oocytes
Laser Tweezer Deformation of Giant Unilamellar Vesicles
Transverse Alternating-Field Electrophoresis
New Fluorescent Substrates of Microbial Transglutaminase and Its Application to Peptide Tag-Directed
Elucidating Protein: DNA Complex by Oligonucleotide DNA Affinity Purification
Combined Biophysical and Cell-Based Approaches for the Assessment of Ligand Binding to PPAR
Combined Biophysical and Cell-Based Approaches for the Assessment of Ligand Binding to PPAR
Chromatin Immunoprecipitation Analysis of NFB Transcriptional Regulation by Nuclear IB in Human Macr
A Computational Approach to Predict Suitable Target Sites for trans-Acting Minimal Hammerhead Ribozy
Structural Determination of O-Glycans by Tandem Mass Spectrometry
Trans-Splicing Reactions by Ribozymes
Isotopic Efflux Studies as Indices of Phospholipase Activation
Noninvasive Bioluminescent Imaging of Infections
Fluorescence Approaches to Growing Macromolecule Crystals
Extraction of Cellular and Tissue RNA
EPR Measurements of Nitric Oxide-Induced Chromanoxyl Radicals of Vitamin E: Interactions with Vitami
Experimental Approaches for Measuring Reaction Parameters
Imaging Phosphoinositide Dynamics in Living Cells
Automated Recombinant Protein Expression Screening in Escherichia coli
Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use
An in vitro method to select malignant cells from surgical biopsies of breast cancer patients
How to Perform a Nanoindentation Experiment on a Virus
Inactivation of sortilin (a novel lysosomal sorting receptor) by dominant negative competition and R
组织培养-TISSUE CULTURE STOCK SOLUTIONS AND MEDIA
Fluorescent Sample Labeling for DNA Microarray Analyses
Immunohistochemical Localization of NOS Isoforms
Expression and purification strategies for the production of single-chain antibody and T-cell recept
Method for introducing specific and unmarked mutations into the chromosome of Streptococcus pneumoni
Dereplication and Partial Identification of Compounds
Large-scale immunofluorescence
High-copy cDNA amplification of minimal total RNA quantities for gene expression analyses
Isolation of LDL(Low Density Lipoprotein) From Human Plasma:
Techniques of EMG signal analysis: detection, processing, classification and applications (Correctio
Nutritional Value of d-Amino Acids, d-Peptides, and Amino Acid Derivatives in Mice
Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Protein Staining Procedures
Subtilisin-Catalyzed Glycopeptide Condensation
Novel Eicosanoids: Isoprostanes and Related Compounds
Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis
Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresis
Cryopreservation of Fish Sperm
Using Charge Ladders and Capillary Electrophoresis to Measure the Charge, Size, and Electrostatic In
Solid-Phase Extraction in Packed Beds on Glass Microdevices
Conjugates of Peptides and Proteins to Polyethylene Glycols
Synthesis of -Vinyl Amino Acids
On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electr
The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization
Nucleic Acid Library Construction Using Synthetic DNA Constructs
Microfluidic Chips Designed for Measuring Biomolecules Through a Microbead-Based Quantum Dot Fluores
Synthesis and Use of Pseudopeptides Derived from 1,2,4-Oxadiazole-, 1,3,4-Oxadiazole-, and 1,2,4-Tri
Chemical Production of Bispecific Antibodies
DNA Separation Mechanisms During Electrophoresis
Frozen Protein Arrays
Characterization of E. coli Ribosomal Particles: Combined Analysis of Whole Proteins by Mass Spectro
Ultramicrotomy for Biological Electron Microscopy
The Structural Biology and Genomics Platform in Strasbourg: an Overview
Lyophilization of Proteins
Overview of the Pipeline for Structural and Functional Characterization of Macrophage Proteins at th
Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device
Protein Lipidation
Microscale Integrated Sperm Sorter
Linkers, Resins, and General Procedures for Solid-Phase Peptide Synthesis
Adrenocorticotrophic Hormone and Related Peptides
Phage-Display Libraries of Murine and Human Antibody Fab Fragments
Processing Plant Tissues for Ultrastructural Study
Chemokine Receptor Antagonist Development
Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt
Cryopreservation of Avian Spermatozoa
On-Resin Convergent Synthesis of a Glycopeptide from HIV gp120 Containing a High Mannose Type N-Link
Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprintin
Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes
Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scatte
Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish
Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-Capillary Deriv
Troubleshooting
Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells
Use of Natural or Selected Mutants and Variants for Epitope Mapping
Principles of Liquid Chromatography
Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions
In vitro transcription systems from cultured cells and fat-body tissue of the silkworm,Bombyx mori
Science through the internet: Researching, evaluating and citing websites
Cytofluorescence Techniques for the Visualization of Distinct Pools of Protein Thiols at the Single
GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in
Special Problems with the Extraction of Plants
Simultaneous Measurement of Mitochondrial Function and NO
Array-Based Nuclear Run-On Analysis
Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA
Capillary Gel Electrophoresis
Methods for the Implantation of Liver Cells
Approaches to Investigate the Role of Signaling in ER-to-Golgi Transport
Basic Techniques in Molecular Biology
Sequence Analysis of Oligodeoxyribonucleotides
Effect of Carbon Nanotubes on HepG2 Adhesion and Spreading
One-Step One-Lane Chemical Sequencing of DNA
RCA-Enhanced Protein Detection Arrays
Plasmid Screening Using Single Colony Lysates
Morphological Analysis of the Zebrafish Digestive System
Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy
Measurement of Carotenoids in the Living Primate Eye Using Resonance Raman Spectroscopy
Screening of compounds toxicity against human Monocytic cell line-THP-1 by flow cytometry
In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System
Erythropoietin and Cytoprotective Cytokines in Experimental Traumatic Brain Injury
A Voluntary Oral-feeding Rat Model for Pathological Alcoholic Liver Injury
Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer
Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids
Tricyclic Antidepressants
Synthesis of Peptides Using Tert-Butyloxycarbonyl (Boc) as the -Amino Protection Group
Synthesis of Cyclopropane-Containing Leu-Enkephalin Analogs
Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries
Gene Probes
Laser Ablation as a Fabrication Technique for Microfluidic Devices
Direct DNA Sequencing of Polymerase Chain Reaction Products Using Magnetic Beads
Electron Microscopic Methods for the Demonstration of Lectin-Binding Sites in Cancer Cell Lines
Enhanced DNA transfer into fish bone cells using polyethylenimine
Hybridization of Enzyme-Labeled Probes and Detection by Chemiluminescence
A Conformationally Restricted -Strand HIV Protease Inhibitor
Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA
PNA Openers and Their Applications for Bacterial DNA Diagnostics
A method to identify p62s UBA domain interacting proteins
Yeast Two-Hybrid Library Screening
Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays
Inserting and Manipulating DNA in a Nanopore with Optical Tweezers
Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes
Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
Resin Technologies: Construction and Staining of Resin TMAs
Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products
Detection of ERK1/2 Activities Using Affinity Reagents
Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biologic
On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Ca
The Isolation of Minicells
Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Control
Scale-Up of Natural Products Isolation
Gene Isolation by Exon Trapping
Analysis of Yeast Artificial Chromosome Clones
RNA Expression Analysis on Formalin-Fixed Paraffin-Embedded Tissues in TMA Format by RNA In Situ Hyb
Digoxigenin Labeling of RNA Transcripts from Multi- and Single-Locus DNA Minisatellite Probes
Oligonucleotide PRINS DNA Synthesis
Analysis of Tat Targeting Function and Twin-Arginine Signal Peptide Activity in Escherichia coli
2-D Diagonal Gel Electrophoresis
T7 Transcript Length Determination Using Enzymatic RNA Sequencing
Production of Double-Stranded cDNA for Gene Library Synthesis
2-O,4-C-Methyleneoxymethylene Bridged Nucleic Acids (2,4-BNACOC)
Mitochondrial Membrane Potential () Fluctuations Associated with the Metabolic States of Mitochondri
Cytochemical techniques and energy-filtering transmission electron microscopy applied to the study o
Isolation, growth and identification of colony-forming cells with erythroid, myeloid, dendritic cell
Glycoprotein Analysis by Capillary Zone Electrophoresis-Electrospray Mass Spectrometry
YAC Library Screening: Preparation of Hybridization Filters and Polymerase Chain Reaction Pools
Detection of hCG responsive expression of the steroidogenic acute regulatory protein in mouse leydig
Deletion of Genes From the Mouse Genome Using Cre/loxP Technology
Solid-Phase Supports for Oligonucleotide Synthesis
Whole-Organism Screening: Plants
Preparing 2-D Protein Extracts from Caenorhabditis elegans
O-Glycoproteomics: Site-Specifi c O-Glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem
Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring a
An Asymmetric Synthesis Protocol to Prepare Topographically Constrained -Substituted Aromatic Amino
Analysis of Organelles by On-Line Two-Dimensional Liquid ChromatographyTandem Mass Spectrometry
Organizing Bioactive Compound Discovery in Target Families
Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry
Nucleic Acid Sequence Analysis Using DNAzymes
Messenger RNA Fractionation on Neutral Sucrose Gradients
Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP
Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
S1 Mapping Using Single-Stranded DNA Probes
RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer
Posttranslational Lipid Modification of Rho Family Small GTPases
One-Tube RT-PCR with Sequence-Specific RT Primers
The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotrop
Phosphoinositide (PI) 3-Kinase Assays
FRAP-Based Analysis of Rho GTPase-Dependent Polar Exocytosis in Pollen Tubes
Cleavage of Compounds from Solid Phase by Gaseous Reagents
Absorption Enhancement by Lectin-Mediated Endo- and Transcytosis
Short Double-Stranded Ribonucleic Acid as Inhibitor of Gene Expression by the Interference Mechanism
Quality Aspects of TMA Analysis
Detection of Specific DNA SequencesThe Southern Transfer
Addressing fluorogenic real-time qpcr inhibition using the novel custom excel file system FocusField
Applications of Lectin Histochemistry and Cytochemistry in Diagnosis and Prognosis
Automated Structure Verification of Small Molecules Libraries Using 1D and 2D NMR Techniques
HPLC and HPAEC of Oligosaccharides and Glycopeptides
Lateral Diffusion Coefficients of Raft Lipids From Pulsed Field Gradient NMR
Antibody Purification by Column Chromatography
Manipulation of Baculovirus Vectors
Fractionated Extraction of Total Tissue Proteins from Mouse and Human for 2-D Electrophoresis
In Vitro Screening for Antioxidant Activity
Inhibition of HIV Infection by Lectin Binding to CD4
The production of monoclonal antibody by hybridoma
In Vitro Transcriptions
Hybridization and Detection of Digoxigenin Probes on RNA Blots
Assay of Retinol-Binding ProteinTransthyretin Interaction and Techniques to Identify Competing Ligan
Characterization of RNase P RNA Activity
Chiral Ligand-Exchange Capillary Electrophoresis and Capillary Electrochromatography
LIBS-Based Detection of Antioxidant Elements: A New Strategy
Microwave Paraffin Techniques for Botanical Tissues
Polypyrrole-Oligosaccharide Microarray for the Measurement of Biomolecular Interactions by Surface P
Polymerase Chain Reaction on Microchips
In Situ PCR Amplification of Intracellular mRNA
Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products
Polymerase Chain Reaction: Basic Principles and Routine Practice
Toward Effective PCR-Based Amplification of DNA on Microfabricated Chips
HPLC of DNA and PCR Products
Production of Hybridization Probes by the PCR Utilizing Digoxigenin-Modified Nucleotides
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-1
Purification of PDE6 Isozymes From Mammalian Retina
Inositol 1,4,5-Trisphosphate Mass Assay
Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) Mass Measurement Using a Radioligand Disp
Expression and Purification of Recombinant Proteins Using the pET System
In Vivo Measurement of Tissue Oxygen Using Electron Paramagnetic Resonance Spectroscopy with Oxygen-
Study of MAPK Signaling Using Knockout Mice
Post-electrophoretic identification of oxidized proteins
Immunohistochemical Detection of Protein Oxidation
Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies
Functional Identification of Bacterial Glucosyltransferase WbdN
A High Throughput Platform for Eukaryotic Genes
Virtual Expert Mass Spectrometrist v3.0: An Integrated Tool for Proteome Analysis
Phosphoinositide-Specific Phospholipase C 1 Signal Transduction in the Nucleus
Real-Time, In-Vivo Measurement of Nitric Oxide Using Electron Paramagnetic Resonance Spectroscopic A
Immunocytology using the HA epitope
Derivatization of Carbohydrates
Use of Denaturing High-Performance Liquid Chromatography in Molecular Medicine
Automation of MLST using third-generation liquid-handling technology
Lectin-Triggered Superoxide/H2O2 and Granule Enzyme Release from Cells
Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum
Ras Signaling Pathway for Analysis of Protein-Protein Interactions in Yeast and Mammalian Cells
Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitoch
Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation, and Recovery of Cellular [3H]Ph
Nanodroplet Chemical Microarrays and Label-Free Assays
Trapping Oxidative Folding Intermediates During Translocation to the Intermembrane Space of Mitochon
Targeting mRNAs by Engineered Sequence-Specific RNase P Ribozymes
Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-Oligomers
PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE)
Proteolytic Labeling With 18O for Comparative Proteomics Studies: Preparation of 18O-Labeled Peptide
Use of Radiation Hybrid panels to map genetic loci
Characterization of Protein Glycosylation
Chemical and Enzymatic Release of Glycans from Glycoproteins
Screening of Pirkle-Type Chiral Stationary Phases for HPLC Enantioseparations
Application of Capillary Electrophoresis to Pharmaceutical Analysis
Determining Lipase Subunit Structure by Sucrose Gradient Centrifugation
Antineoplastic Drugs
High-Level Baculoviral Expression of Hormone-Sensitive Lipase
Liquid Chromatography Column Technology
Biochemical determination of natural tumor-associated T-cell epitopes
Epitope Mapping on Extracellular Domains of Cell-Surface Proteins Using Exonuclease III
Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
Isolation of Total Cellular DNA from Tissues and Cultured Cells
The Isolation of High Molecular Weight Eukaryotic DNA
Evaluating phenotype and genotype of drug-resistant strains in herpesviruses
Quantitative TaqMan Real-Time PCR: Diagnostic and Scientific Applications
Invader Assay for Single-Nucleotide Polymorphism Genotyping and Gene Copy Number Evaluation
Generation of Large Insert YAC Libraries
Analysis of Gene Sequences by Hybridization of PCR-Amplified DNA to Covalently Bound Oligonucleotide
A simple method for the production of highly competent cells ofAgrobacterium for transformation via
The metabolic inhibition model which predicts the intestinal absorbability and Metabolizability of d
Internet Resources for the Antibody Engineer
Analysis of Receptor Tyrosine Kinase Internalization Using Flow Cytometry
Capillary DNA-Protein Mobility Shift Assay
Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays: Hydrogel Immunoassay
Nitric Oxide Imaging in Neurons Using Confocal Microscopy
Structural Genomics of Minimal Organisms: Pipeline and Results
Machine Learning: An Indispensable Tool in Bioinformatics
Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation, Purification, and Characterization of
In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polyp
Antibody Affinity Maturation by Random Mutagenesis
Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology
Methods for Analysis of Unusual Forms of O-Glycosylation
In Vitro Translation of mRNA in a Rabbit Reticulocyte Lysate Cell-Free System
Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining
Parkinsons Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin
Nuclear Magnetic Resonance (NMR)-Based Drug Metabolite Profiling
Rapid identification of differentially expressed genes by in situ screening of bacteria
Antibody Epitope Mapping Using Arrays of Synthetic Peptides
Single-Strand Conformation Polymorphism (SSCP) Analysis
High-resolution glycoprotein analysis using capillary electrophoresis
Procedures for the Analysis and Purification of His-Tagged Proteins
Crystallization and Final Stages of Purification
Enhancement of Ribozyme Function by RNA Binding Proteins
Minimized Hammerhead Ribozymes
Design of the Hairpin Ribozyme for Targeting Specific RNA Sequences
Design and Optimization of Sequence-Specific Hairpin Ribozymes
Formation and Characterization of PNA-Containing Heteroquadruplexes
Bioluminescent Assay of the Guanylates
Antibody Humanization by CDR Grafting
Cloning DNA Fragments in M13 Vectors
Analytical Methods and Steps to Sample Preparation for Determination of Molecular Species of Fatty A
马血清在细胞生物学领域的作用
Gene Therapy in Cancer Via Use of a Retrovector Having a Tumor Specificity and Expressing Inducible
Quantitative Measurements
Antibody Conjugation Protocol
DABA Fluorescence Assay for Submicrogram Amounts of DNA
Microfluorometric Measurement of the Formation of All-Trans-Retinol in the Outer Segments of Single
Nitrocellulose-Based Immunoaffinity Chromatography
Application of Direct Aqueous Supercritical Fluid Extraction for the Dynamic Recovery of Testosteron
Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosol
Improvements in the Application of Firefly Luciferase Assays
Quantitating Defective Ribosome Products
Small-Molecule Inhibitors of Proteasome Activity
Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluo
Laurdan Studies of Membrane Lipid-Nicotinic Acetylcholine Receptor Protein Interactions
Detecting, Visualising, and Quantifying Mucins
Antibodies in Proteomics
Photoreceptor Degeneration in Mice: Adeno-Associated Viral Vector-Mediated Delivery of Erythropoieti
Three-Dimensional Reconstruction of Chromosomes Using Electron Tomography
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
T-Cell Epitope Mapping with Synthetic Peptides and Peripheral Blood Mononuclear Cells
Tracking the Transport of E-Cadherin to and From the Plasma Membrane
Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA
Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses
Characterization of HIV gp120 Envelope Glycoprotein by Lectin Analysis
Digital Microscopy for Boosting Database Integration and Analysis in TMA Studies
Real-Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase
Use of NO Donors in Biological Systems
Application of Dual Cyclodextrin Systems in Capillary Electrophoresis Enantioseparations
Synthesis, Mass Spectrometric Characterization, and Analysis of the PPAR Agonist GW1516 and Its Majo
Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling
Determining Allosteric Modulator Mechanism of Action: Integration of Radioligand Binding and Functio
Practical Considerations of Image Analysis and Quantification of Signal Transduction IHC Staining
A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo Oligonucleotide Delivery
Saturation-Recovery Electron Paramagnetic Resonance Discrimination by Oxygen Transport (DOT) Method
The Use of Restriction Endonucleases
A screen to identify drug resistant variants to target-directed anti-cancer agents
In Vitro Selection of Metal Ion-Selective DNAzymes
Photorhabdus luminescens luxCDABE Promoter Probe Vectors
Preparation of Direct, Enzyme-Labeled DNA Probes
Preparation of Horseradish Peroxidase-Labeled Probes
Synthesis of (2R, 3R, 4R, 5S)-5-tert-Butyloxycarbonylamino-3,4-Dihydroxy-2-1sopropyl-3,4-O,O-Isoprop
Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors
Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism
Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis
Chiral Separation Principles: An Introduction
Generation of PDE4 Knockout Mice by Gene Targeting
Automated Liquid-Liquid Extraction and Ion-Exchange Solid-Phase Extraction for Initial Purification
In Vivo ChIP for the Analysis of Microdissected Tissue Samples
In Vivo Screening of Ligand-Dependent Hammerhead Ribozymes
Using the Flp-In T-Rex System to Regulate GPCR Expression
An Overview of CE in Clinical Analysis
On-Line SFE-SFC for the Analysis of Fat-Soluble Vitamins and Other Lipids from Water Matrices
Southern Blotting of Agarose Gels by Capillary Transfer
Double-Label Analysis
Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS
Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains
Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides
Design and Synthesis of Antioxidant -Lipoic Acid Hybrids
tRNA Delivery Systems for Ribozymes
Regulation of MAPK Cascades by Protein Tyrosine Phosphatases
Evaluating Effects of EPO in Rodent Behavioral Assays Related to Depression
Initiation, Maintenance, and Quantification of Human Hematopoietic Cell Cultures
Increasing Protein Crystallization Screening Success with Heterogeneous Nucleating Agents
Cryopreservation of Mammalian Embryos
Cryopreservation of Desiccation-Tolerant Seeds
Visualizing Intracellular Distribution and Activity of Core2 (1,6)N-Acetylglucosaminyltransferase-I
Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format
Tissue Microarrays from Biopsy Specimens
Smart Polymer-Streptavidin Conjugates
Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates
Preparation of Membrane Rafts
Screening Recombinant Libraries by Polymerase Chain Reaction
The Human Genome Project
A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Kno
Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis
Analyzing PPAR/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry
Plasmid DNA Isolation by the Cleared Lysate Method
Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevi
Indirect Enantioseparation by HPLC Using Chiral Benzofurazan-Bearing Reagents
Synthesis by high-efficiency liquid-phase (HELP) method of oligonucleotides conjugated with high-mol
Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes
O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Ran
Affinity Measurement Using Surface Plasmon Resonance
Protocol for Ex Vivo Incubation of Drosophila Primary Post-embryonic Haemocytes for Real-Time Analys
A method for analyzing the ubiquitination and degradation of aurora-A
Design and Preparation of Sequence-Specific RNase P Ribozymes
Blot Analysis with Lectins for the Evaluation of Glycoproteins in Cultured Cells and Tissues
Fluorogenic Analysis of H2O2 in Biological Materials
R Classes and Methods for SNP Array Data
Assembly of PNA-Tagged Small Molecules, Peptides, and Carbohydrates onto DNA Templates: Programming
Affinity Capillary Electrophoresis of Oligosaccharides
Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System
Generation of baculovirus expression vectors
Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments. Part I: Array Printin
Microwave Fixation of Rat Hippocampal Slices
Fluorometric Assay for Detection of Sterol Oxidation in Liposomal Membranes
Precise large deletions by the PCR-based overlap extension method
Microwave Processing Techniques for Biological Samples in a Service Laboratory
Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter Groups
Nucleotide Exchange Factors for Hsp70 Chaperones
Direct application of plasmid DNA containing type I interferon transgenes to vaginal mucosa inhibits
Lipases as Catalysts in Synthesis of Fine Chemicals
Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes
Introduction of Hypermodified Nucleotides in RNA
Northern Blot Analysis
Simultaneous RNA and DNA Extraction from Biopsy Material, Culture Cells, Plants, and Bacteria
RAPD Assay: A Novel Technique for Genetic Diagnostics
Analysis of Carbohydrates by Mass Spectrometry
High-Throughput Genotyping With Energy Transfer-Labeled Primers
$The Amplification Refractory Mutation System$
The Amplification Refractory Mutation System
Detection of NOS Isoforms by Western-Blot Analysis
The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection
Determination of Tyrosine and Tryptophan Metabolites in Body Ruids Using Electrochemical Detection
Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using t
Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel E
Purification and Enzymatic Sequencing of Polymerase Chain Reaction Products
Generation of Epitope-Tagged GPCRs
Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry
The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling
Systemic Delivery of Synthetic siRNAs
Spectrophotometric Analysis of Nucleic Acids
Analysis of Urinary 8-oxo-7,8-dihydro-2-deoxyguanosine by Liquid ChromatographyTandem Mass Spectrome
Directed Mutagenesis of Antibody Variable Domains
Tobramycin Affinity Tag Purification of Spliceosomes
Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template
Improved method for generating microarray probes using submicrogram amounts of total RNA
Peptide Nucleic Acid-Mediated Recombination for Targeted Genomic Repair and Modification
Site-Directed Mutagenesis
Restriction Endonuclease Digestion of DNA
Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels
Target-Site Selection for the 1023 DNAzyme
An efficient ligation method in the making of an in vitro virus for in vitro protein evolution
Application of a colorimetric assay to identify putative ribofuranosylaminobenzene 5-phosphate synth
Analysis of Average Telomere Length in Cultured Human Cells
Introduction to Telomeres and Telomerase
Probing the Telomere Damage Response
Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
Probing Antibody-Antigen Interactions by Mass Spectrometry
Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute
Tandem Mass Spectrometry in the Detection of Inborn Errors of Metabolism for Newborn Screening
Interpretation of Collision-Induced Fragmentation Tandem Mass Spectra of Posttranslationally Modifie
TAL Effector Nuclease (TALEN) Engineering
Integration of Phosphodiesterase-Induced Degradation of Oligonucleotides with Capillary Polymer-Siev
Transcription In Vitro Using Bacteriophage RNA Polymerases
Chemical and Enzymatic Approaches to Construct Modified RNAs
Surfactant-Based Methods for Prevention of Protein Adsorption in Capillary Electrophoresis
Fabrication of Carbohydrate Surfaces by Using Non-derivatised Oligosaccharides
SPR/MS: Recovery from Sensorchips for Protein Identification by MALDI-TOF Mass Spectrometry
CarbohydrateLectin Interactions Assayed by SPR
A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surfa
Surface Plasmon Resonance Spectroscopy for Studying the Membrane Binding of Antimicrobial Peptides
Incorporation of a Transmembrane Protein into a Supported 3D-Matrix of Liposomes for SPR Studies
RNAi Expression Vectors in Plant Cells
Supercritical Fluid Extraction
Supercritical Fluid Extraction of Nitrosamines from Cured Meats
Separation of Racemic 1-(9-Anthryl)-2,2,2-trifluoroethanol by Sub-/Supercritical Fluid Chromatograph
Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
Retroviral Delivery and Anti-HIV Testing of Hammerhead Ribozymes
Studying Extracellular Signaling Utilizing a Glycoproteomic Approach: Lectin Blot Surveys, a First a
The Extraction of Total RNA by the Detergent and Phenol Method
Tissue Sample Collection for Proteomics Analysis
Subtraction Hybridization cDNA Libraries
Analysis of among-site variation in substitution patterns
Artificial Pancreas to Treat Type 1 Diabetes Mellitus
PPAR SUMOylation: Some Useful Experimental Tips
On the Discovery of Precursor Processing
Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded K
Simultaneous Analysis of Multiple Redox-Active Metabolites from Biological Matrices
Lipases as Biocatalysts for the Synthesis of Structured Lipids
Structure-Based Library Design in Efficient Discovery of Novel Inhibitors
Determination of Lipid Spontaneous Curvature From X-Ray Examinations of Inverted Hexagonal Phases
Small-Angle Scattering and Neutron Contrast Variation for Studying Bio-Molecular Complexes
Designability and Disease
Methods for direct determination of mitomycin C in aqueous solutions and in urine
Fluorescence Microscopy of Nanochannel-Confined DNA
Analysis of Heat-Shock Transcription Factor and Element-Binding Activity
Statistical Methods in Research
Whole-Mount Immunoelectron Tomography of Chromosomes and Cells
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
Sphingomyelin Quantitation Post-choline Labeling of HL60 Cells
Sphingomyelin Mass Measurement
电镜样品制备方法 Specimen preparation for electron microscopy
Crosslinking of Single-Stranded DNA to Resins
The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM) Purging and Hematopoietic Progenitor Cell E
Separation and Size Determination of DNA over a 10,200 kbp Range
Solubilization and Assay of Cellular and Tissue Protein
Crystallographic Analyses of Chemically Synthesized Modified Hammerhead RNA Sequences as a General A
Western Immunoblot Analysis
Neurophenotyping Genetically Modified Mice for Social Behavior
High-Throughput Screening of Model Bacteria
Mechanistic Analysis of the Hepatitis Delta Virus (HDV) Ribozyme: Methods for RNA Preparation, Struc
Rho GTPases and Cancer Cell Transendothelial Migration
Preparation of Photo-Cross-Linked Small Molecule Affinity Matrices for Affinity Selection of Protein
Potential Design Rules and Enzymatic Synthesis of siRNAs
RNA Interference (RNAi) With RNase III-Prepared siRNAs
Target-Induced SOFA-HDV Ribozyme
Point Mutation Detection by Temperature-Programmed Capillary Electrophoresis
Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scatter
Yeast exoglycoproteins produced under NaCl-stress conditions as efficient cryoprotective agents
Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast
Chemoselective Protein and Peptide Immobilization on Biosensor Surfaces
Site-Selective Scission of Human Genome Using PNA-Based Artificial Restriction DNA Cutter
Characterization of Interactions Between Proteins Using Site-Directed Spin Labeling and Electron Par
Protein Engineering: Single or Multiple Site-Directed Mutagenesis
Primer-Directed Site-Specific Mutagenesis
Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Al
Single Enzyme Studies: A Historical Perspective
DNA-Templated Native Chemical Ligation of Functionalized Peptide Nucleic Acids: A Versatile Tool for
Ligation of DNA with T4 DNA Ligase
Yeast Artificial Chromosomes
Separation of Enantiomers by Capillary Electrophoresis Using Cyclodextrins
Using a Noncovalent Protection Strategy to Enhance Solid-Phase Synthesis
Weak Affinity Chromatography
Inverse Polymerase Chain Reaction
Development of a High-Throughput Capillary Electrophoresis Protocol for DNA Fragment Analysis
Production of Panels of Monoclonal Antibodies by the Hybridoma Method
Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting
Mapping of Signaling Pathways by Functional Interaction Proteomics
General Consideration on Sialic Acid Chemistry
Shotgun phage display Selection for bacterial receptins or other exported proteins
Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes Using Tempo
Cryopreservation of Shoot Tips and Meristems
Oscillating Probe for Dual Detection of 5'PO4 and 5'OH DNA Breaks in Tissue Sections
Nonradioactive Northern and Southern Analyses From Plant Samples
Use of three-dimensional collagen gels to study mechanotransduction in t47d breast epithelial cells
Dealing with different methods for Kluyveromyces lactis -galactosidase purification
Formation and Characterization of Hepatocyte Spheroids
Ubiquitin Chain Synthesis
High-Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment
Nonreducing 2-D Polyacrylamide Gel Electrophoresis
CE Analysis of -Globulin Mobility and Potential Clinical Utility
Preparation and Assay of Recombinant Serine Racemase
Serglycin: The Master of the Mast Cell
On-Bead and Solution Screening Approaches for Genomically Derived Targets: Discovery of Surrogate Li
Sequence-Specific DNA Labeling Using Methyltransferases
HPLC Analytical Methods for the Separation of Molecular Species of Fatty Acids in Diacylglycerol and
Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry of Amino Acids, Peptides, and Pr
Covalent Coupling of DNA Oligonucleotides and Streptavidin
Oligo(dT)-纤维素层析分离Poly(A)+ RNA
Development of an On-Site Measurement System for Salivary Stress-Related Substances Based on Microch
Gel-Forming and Cell-Associated Mucins: Preparation for Structural and Functional Studies
Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols
Dideoxyfingerprinting for Mutation Detection
Using RNA Interference in Schistosoma mansoni
Evaluation of intra- and interspecific divergence of satellite DNA sequences by nucleotide frequency
Capillary Electrophoresis with Laser-Induced Fluorescence Detection of Proteins from Two Types of Co
Determination of Mono-, Di-, and Oligosaccharides by Capillary Electrophoresis with Capacitively Cou
Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity
Interactions Between Catalytic and Inhibitory Subunits of PDE6
General Design and Construction of RNase P Ribozymes for Gene-Targeting Applications
Genome Wide Full-Length Transcript Analysis Using 5 and 3 Paired-End-Tag Next Generation Sequencing
Probing the Mechanics of the Complete DNA Transcription Cycle in Real-Time Using Optical Tweezers
A rapid and sensitive assay for quantification of siRNA efficiency and specificity
Quantification of siRNAs In Vitro and In Vivo
Nonradioactive Methods for the Detection of RNA-Protein Interaction
Preparation of RNA Gel Blots
Preparation of RNA Dot Blots
Quantitation of Ribozyme Target Abundance by QCPCR
Computer-Aided Calculation of the Local Folding Potential of Target RNA and Its Use for Ribozyme Des
Maxizyme Technology
Ligation of RNA Molecules by the Hairpin Ribozyme
Crystallographic Analysis of Small Ribozymes and Riboswitches
Ribozyme as an approach for growth suppression of human pancreatic cancer
Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide Duplexes
Analysis of the Role of RhoGDI1 and Isoprenylation in the Degradation of RhoGTPases
Analysis of Rho GTPase Activation in Saccharomyces cerevisiae
Assessing Ubiquitylation of Rho GTPases in Mammalian Cells
Rho GTPases: Deciphering the Evolutionary History of a Complex Protein Family
Assessment of Rho GTPase Signaling During Neurite Outgrowth
Biochemical Assays to Characterize Rho GTPases
High-Throughput Flow Cytometry Bead-Based Multiplex Assay for Identification of Rho GTPase Inhibitor
Reversed-Phase HPLC
Analysis of the Citric Acid Cycle Intermediates Using Gas Chromatography-Mass Spectrometry
Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition
Synthesis of 3-Amino-l-CarboxymethyI-Benzodiazepine (BZA) Peptidomimetics
Determination of NO with a Clark-Type Electrode
Purification of the Inducible Nitric Oxide Synthase
Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis
Baculovirus Vectors
Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria
Retroviral-mediated gene transfer in primary murine and human T-lymphocytes
A method for rapidly screening functionality of actin mutants and tagged actins
Site-Specific Immobilization of Antibodies to Protein G-Derivatized Solid Supports
Analysis of Triplet-Repeat DNA by Capillary Electrophoresis
Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphoryl
Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry for Protein and Peptide Profiling of
A Brief Summary of the Different Types of Mass Spectrometers Used in Proteomics
Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation
Nanoporous Silica Glass for the Immobilization of Interactive Enzyme Systems
Methods for analysis of matrix metalloproteinase regulation of neutrophil-endothelial cell adhesion
Differential Display: Theory and Applications
Lipase and Phospholipase Biosensors: A Review
Quantum Dot Labeling Strategies to Characterize Single-Molecular Motors
Improving image analysis in 2DGE-based redox proteomics by labeling protein carbonyl with fluorescen
Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries
Purification of Water-Soluble Natural Products
TaqMan Reverse Transcriptase-Polymerase Chain Reaction Coupled With Capillary Electrophoresis for Qu
Rapid and simple comparison of messenger rna levels using real-time PCR
Real-Time Monitoring the Spatiotemporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cel
In vitro Sphingomyelinase Assay
Indirect Immunofluorescence
Phosphate Assay by Suprya Jaydev
DGK Membrane Preparation
Protein A Purification of Antibody
Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phos
Electron Paramagnetic Resonance Oximetry and Redoximetry
Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo
常用实验动物全身性麻醉药物的使用
三溴乙醇（阿佛丁）麻醉剂问题解答（一）
Detection of Reactive Oxygen Species by Flow Cytometry
Nitrite Determination by Colorimetric and Fluorometric Griess Diazotization Assays: Simple, Reliable
RNA Oligonucleotide Synthesis Via 5-Silyl-2-Orthoester Chemistry
High-Throughput Quantitation of Metabolically Labeled Anionic Glycoconjugates by Scintillation Proxi
Analytical Methods for Optimization and Quality Control of Combinatorial Synthesis
Toxicology Screening
Radioimmunoassay for the Quantification of cGMP Levels in Cells and Tissues
Radiolabeling of Peptides and Proteins
Analysis of Aliphatic Amino Acid Alcohols in Oxidized Proteins
Assessment of the Role for Rho Family GTPases in NADPH Oxidase Activation
Construction of a bacterial autoinducer detection system in mammalian cells
Quantitative TaqMan Assay for the Detection and Monitoring of Cytomegalovirus Infection in Organ Tra
Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardio
Multispectral Imaging and Automated Laser Capture Microdissection of Human Cortical Neurons: A Quant
Capture of Intact Liposomes on Biacore Sensor Chips for ProteinMembrane Interaction Studies
A novel method for preparing single-stranded DNA for pyrosequencing
Radioiodination of protein
分离人单核细胞和中性粒细胞-Purification of human mononuclear cells and neutrophi
The influence of photoperiodic growth condition on isolation of RNA from strawberry (Fragaria ananas
$Purification and cloning of DNA fragments fractionated on agarose gels$
Purification and cloning of DNA fragments fractionated on agarose gels
Theories of Pulsed-Field Gel Electrophoresis
Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA
Protozoan Genomes: Karyotype Analysis, Chromosome Structure, and Chromosome Specific Libraries
Genotyping for Cytochrome P450 Polymorphisms
Site-Directed Mutagenesis with LA-PCR Technology
Identification of Components of Protein Complexes
Sphingomyelin Mass Measurement
免疫荧光操作（共聚焦显微镜）-Immunofluorescence Protocol for Confocal Microscopy
Small Molecule Microarray: Functional-Group Specific Immobilization of Small Molecules
Absolute Quantitation of 2-D Protein Spots
Modulatory Effects of Proteoglycans on Proteinase Activities
Models for Studies of Proteoglycans in Kidney Pathophysiology
Protein Subcellular Localization Prediction Using Artificial Intelligence Technology
Endoplasmic Reticulum-Associated Protein Quality Control and Degradation: Screen for ERAD Mutants Af
Immunohistochemical Techniques to Identify and Localize Proteins of Interest in Paraffin Embedded Ti
Retention Time Prediction and Protein Identification
Combining genetic and biochemical approaches to identify functional molecular contact points
Measuring Ubiquitin Conjugation in Cells
Measurement of S-Glutathionated Hemoglobin in Human Erythrocytes by Isoelectric Focusing Electrophor
In Vitro and In Vivo Approaches to Studying the Bacterial Signal Peptide Processing
Protein Multiple Sequence Alignment
Application of CGE to Virus Identification
Photoaffinity Labeling of Protein O-Mannosyltransferases of the PMT1/PMT2 Subfamily
Peptide Affinity Purification for the Isolation and Identification of GPCR-Associated Protein Comple
Reconstitution of Active and Stoichiometric Multisubunit Lysine Acetyltransferase Complexes in Insec
A Peptide Microarray for Detecting Protein Kinase Activity in Cell Lysates
Using Hidden Markov Models to Discover New Protein Transport Machines
High Density Peptide Microarrays for Proteome-Wide Fingerprinting of Kinase Activities in Cell Lysat
Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzy
Protein Blotting: Principles and Applications
Production of Protein Arrays by Cell-Free Systems
Capillary Coating for Protein Separation Based on Si-O and Si-C Covalent Bond Formation for Capillar
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts
Using N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) to Assay Cyclooxygenase Activity In Vitro
A Proteomic Protocol to Identify Physiological Substrates of Pro-protein Convertases
The 1,5-Disubstituted Tetrazole Ring as a cis-Amide Bond Surrogate
Detection of Apoptosis Using Cyclic Luciferase in Living Mammals
Plasmid Preparation on Sephacryl S1000
RNA-based Studies Processing of Microdissected Tissue for Molecular Analysis
Crude Extract Immunodepletion
Hybridization of Digoxigenin-Labeled Probes to Southern Blots and Detection by Chemiluminescence
Labeling of Double-Stranded DNA Probes with Biotin
Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides
Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins
SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads
Primer Extension Analysis of mRNA
Methods to Isolate, Culture, and Study Osteoblasts
Pressure Perturbation Calorimetry
Preparation of bOG-DOPG mixed micelles
Preparation and use of Zamboni’s-fixed Frozen Sections in Immunohistochemistry
Free-Flow Electrophoresis of the Human Urinary Proteome
Specimen Collection and Handling: Standardization of Blood Sample Collection
Preabsorbing Antisera with C. elegans Acetone Powder
Fluorescence resonance energy transfer (FRET) as a method to calculate the dimerization strength of
An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes
Analyses of PCSK9 Post-translational Modifications Using Time-of-Flight Mass Spectrometry
Identification of SUMO-Binding Motifs by NMR
Assay and Purification of Calmodulin-Dependent Protein Kinase
RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation-Microarray (Chip) Profiling
Gene-Array Analysis of Glioma Cells After Treatment With an Anti-PKC siRNA: A General Protocol
Dissecting GLUT4 Traffic Components in L6 Myocytes by Fluorescence-Based, Single-Cell Assays
Synthesis of Peptidomimics Through Sugar-Based Scaffolds
One-Step Optimization Using Touchdown and Stepdown Polymerase Chain Reaction
POLY-L-LYSINE COATED SLIDES
Polygalacturonase assay
Enzyme Immobilization in Polyelectrolyte Microcapsules
Polyclonal and Monoclonal Antibodies
Supercritical Fluid Extraction of Polychlorinated Biphenyls from Fish Tissue
Radiochemical Estimation of Polyamine Oxidase
Sample Preparation Methods for Mass Spectrometric Peptide Mapping Directly from 2-DE Gels
Processing of Resorbable Poly--Hydroxy Acids for Use as Tissue-Engineering Scaffolds
秀丽线虫基因敲除方法--C. elegans Gene Knockout Protocol
Extraction and Purification of Plasmid DNA
Plant Transformation
Coupling optical and electrical measurements in artificial membranes: Lateral diffusion of lipids an
Expression L19 using Pichia pastoris
Photolysis of Caged Calcium Using a Low-Cost Flash Unit
Interstitial Photodynamic Therapy with Moving Exposure Fiber
Statistical Analysis: Receiver Operating Characteristic (ROC) Curve and Lipid Peroxidation
Optimized Protocol to Make Phospho-Specific Antibodies that Work
Phospholipases: An Overview
Monitoring of Phospholipase A2 Activation in Cultured Cells Using Tritiated Arachidonic Acid
Phosphatidylinositol 4-Kinases: Assays and Product Analysis
High-Throughput Screening of Phosphodiesterase Activity in Living Cells
Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography
Methods to Assess Changes in the Pattern of Nuclear Phosphoinositides
Lipid Domains in Supported Lipid Bilayer for Atomic Force Microscopy
High Throughput Protein Production and Crystallization at NYSGXRC
Antibody Affinity Maturation by Chain Shuffling
Design of Targeted Libraries Against the Human Chk1 Kinase Using PGVL Hub
Specific Knockdown of PPAR Gene in Colon Cancer Cells by Lentivirus-Mediated RNA Interfering
PPAR-Alpha Cloning, Expression, and Characterization
Analyzing Phosphorylation-Dependent Regulation of Subcellular Localization and Transcriptional Activ
Azatides as Peptidomimetics: Solution and Liquid Phase Syntheses
Peptide Microarrays on Coated Silicon Slides for Highly Sensitive Antibody Detection
Synthesis, Characterization, and Evaluation of Radiometal-Containing Peptide Nucleic Acids
Use of Peptide Nucleic Acids (PNAs) for Genotyping by Solution and Surface Methods
Preparation of Metal-Containing Peptide Nucleic Acid Bioconjugates on the Solid Phase
Antisense Effects of PNAs in Bacteria
Pectinase assay
Extracting Monoisotopic Single-Charge Peaks From Liquid Chromatography-Electrospray Ionization-Mass
PCR的历史
Rapid Expansion of Supercritical Solutions Technology: Production of Fine Particles of Steroid Drugs
石蜡加填充剂 Paraffin Embedding
Assay for high glucose-mediated islet cell sensitization to apoptosis induced by streptozotocin and
Approaches for Studying Nucleosome Movement by ATP-Dependent Chromatin Remodeling Complexes
Oxidative Lipidomics of Apoptosis: Quantitative Assessment of Phospholipid Hydroperoxides in Cells a
Protein Carbonyl LevelsAn Assessment of Protein Oxidation
Measurement of 8-Isoprostane in Exhaled Breath Condensate
Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telom
Ubiquinol/Ubiquinone Ratio as a Marker of Oxidative Stress
Electrochemical Detection of 8-Hydroxy-2-Deoxyguanosine Levels in Cellular DNA
Simultaneous Analysis of Expression of Multiple Redox-Sensitive and Apoptotic Genes in Hypothalamic
Isotope Dilution Gas Chromatography-Mass Spectrometric Analysis of Tyrosine Oxidation Products in Pr
Measurement of F4-Neuroprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ioniz
Antioxidant Activity of Low-Density Lipoprotein
Structural Analysis of Naturally Occurring Sialic Acids
Microwave-Assisted Formalin Fixation of Fresh Tissue: A Comparative Study
Single-Molecule Protein Unfolding and Refolding Using Atomic Force Microscopy
Glycosylated Self-Assembled Monolayers for Arrays and Surface Analysis
Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides
7 Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophore
Unraveling Chemokine and Chemokine Receptor Expression Patterns Using Genetically Engineered Mice
Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System
Chemical Functionalization and Bioconjugation Strategies for Atomic Force Microscope Cantilevers
硫酸盐沉淀
离心的基础知识
基因组DNA提取方法
生物氧化复习笔记
动植物组织mRNA提取
不连续氯化铯密度梯度离心技术高度纯化刚地弓形虫卵囊
质粒DNA的小量制备
TRIzol RNA提取方法
离心分离方法
关于大生产中的离心
生物大分子的离心分离实例
细胞离心技术
离心机原理及概况
离心分离的几种方法
离心技术详解
细胞凋亡检测：生化特征的检测方法
等密度离心(isodensity centrifugation)
大豆分离蛋白的提取方法
Ficoll密度梯度离心法分离外周血单个核细胞
为什么离心的时候离心管会碎呢？
细胞离心
蔗糖密度梯度离心
离心问题
离心转数清洗细胞
能离心吗
质粒提取中各步离心时离心力怎么确定？
离心力的换算
超滤离心
病毒离心纯化方法
CSCL梯度离心
RNA提取中的离心问题
离心参数的选择
包含体离心
FICOLL离心
旋流除尘离心机的技术特点
离心法尿红细胞计数结果准确性的初探
难处理乳化油微波破乳—离心分离技术研究
膜分离技术应用于银杏叶提取黄酮类物质的工艺分析
高速冷冻离心机的使用方法
生产用离心机的转速
病毒RNA提取实验方法（protocol）
密度梯度离心基础知识
血清脂蛋白的离心分离技术
差速离心分离植物（动物）细胞核
离心实验技术基础
离心性收缩（讨论）
移动区带离心(moving-zone centrifugation)
蛋白质特性与分离纯化技术的选择
离心分离方法专题介绍
持续发展的离心分离技术
生物大分子离心分离技术
离心技术基础
脂类代谢复习笔记
食品添加剂-肉桂酸甲酯(结晶体)介绍
对羟基肉桂酸（结晶体）
大豆磷脂与蛋黄磷脂的区别
脂类的种类
Sphingomyelin Mass Measurement 鞘磷脂质量分析
用于给药系统的脂质体表征
大孔吸附树脂吸附分离理论新进展
奶粉中亚硝酸盐检测方案
上海月旭为您提供地沟油检测方案
血清总胆固醇的测定——邻苯二甲醛法
胆固醇在脂质体中的应用
溶解在正己烷中的脂类会随着溶剂一起蒸发掉吗
疏水层析中酯类与疏水性强蛋白的分离
关于膜蛋白和膜脂类溶解问题
正丁醇分离出来的东东有可能是油脂类化合物吗？
如何将脂类从蛋白中去除
脂类提取剂的选择及样品预处理
脂类的测定
LIPID EXTRACTION
肉桂醛的应用
脂肪乳在药物载体中的应用与发展
油红O脂类染色法
动植物组织内脂肪和脂类物质的提取技术
脂类染色
种子粗脂肪的提取
Labeled Sphingomyelin Synthesis 合成标记的神经鞘磷脂
粗脂肪含量的测定——索氏抽提法
新型扫描技术，让“沉默”的肝脏发声
Liposome Preparation
Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
Methylation of Fatty Acids (Kropinski Method)脂肪酸甲基化
Folch method Folch法提取脂类
EXTRACTION AND ANALYSIS OF LIPIDS
Arachidonic Acid Labeling 花生死烯酸标记方法
Desalting Acidic Glycosphingolipids 鞘糖脂脱盐
Glycosphingolipid analysis 鞘糖脂分析
In vitro Sphingomyelinase Assay 体外分析神经磷脂酶
Column Purification of Demethylated Sphingomyelin 柱纯化脱甲基鞘磷脂
Purification of Demethylated Sphingomyelin 纯化脱甲基鞘磷脂
Neutralizing Arachidonic Acid 中和花生四烯酸
脂类测定标准化工作的回顾和建议
脂类染色法
血清脂类测定方法
凝胶延长分析二型脂肪酸合成的方法
LIPID EXTRACTION GENERAL METHODOLOGIES
Liposome Swelling Assay
Planar Lipid Bilayer Experiment平面脂双层实验
离心技术
环肽哪里能测氨基酸绝对构型
生物法将成为已二酸合成技术发展趋势
筛选多肽的操作步骤
关于氨基酸的酯化反应
磁性微粒偶联生物分子的原理以及使用
氨基酸标准品制备
培养基中的氨基酸能高温灭菌吗
氨基酸缩合碰到的问题
氨基酸序列的疏水与亲水的疑问
氨基酸的保护
有关氨基酸旋光性的讨论
氨基酸与铜器皿
氨基酸显色的疑问
氨基酸的冻干问题
提取氨基酸的问题
非必需氨基酸配制
小分子水溶性γ位的氨基酸酯如何水解
氨基酸产物的分离
氨基酸的UV含量测定方法
精氨酸出峰时间
32个氨基酸的短肽检测
氨基酸可以固定吗
氨基酸组分分析
多肽中氨基酸置换问题
氨基酸分析的疑问
氨基酸柱的选择和使用
人体内是否含有D-氨基酸
关于必需氨基酸的讨论
氨基酸测定问题
关于boc-氨基酸做成酰氯的疑问
复方氨基酸和支链氨基酸是怎样制备
氨基酸衍生出现沉淀
关于DL-氨基酸的疑问
氨基酸苄酯化的方法的讨论
微生物利用的氨基酸类型
肠外营养（氨基酸）的热卡计算
氨基酸去保护
氨基酸的发现历史
氨基酸的其他氨基酸检查方法
氨基转移反应的定性鉴定
高效液相测定氨基酸（讨论）
糖代谢复习笔记
“人工叶”太阳能电池模拟自然发电
Oasis HLB净化和ACQUITY UPLC/TQD快速分析动物性食品中糖皮质类激素兴奋剂的含量
蒽酮法测定可溶性糖
Voges-Proskauer(V-P) 试验的原理及注意事项
还原糖和总糖的测定3,5-二硝基水杨酸比色法
直链淀粉和支链淀粉的测定(双波长法)
微生物的糖发酵
利用离子液体可使果糖催化脱水成为HMF
生化与细胞所等发现索拉菲尼改善肝纤维化的新机制
用于石蜡切片的骨相关酶（TRAP、ALP）的双重染色法
酶标抗体的质量鉴定与保存
酶的提取和分离纯化
从环境基因组扩增脂肪酶片段的一些问题
酶的生产方法介绍
酶的载体结合法交联法包埋法等固定化方法
他汀类药物市场浅析
激酶磷酸化酶磷酸酯酶有什么区别？
果胶酶用什么溶解
α-淀粉酶的性质
纤维素酶水解黄酮苷的操作
测定脂肪酶活性灵敏方法
巨噬细胞和淋巴细胞非特异性酯酶染色的方法
脂肪酶的检测
关于胰蛋白酶
醛糖还原酶测定
用胰蛋白酶切蛋白需要加氯化钙吗？
纤维素酶酶活的测定方法
蛋白酶活性与PH值的关系
脂肪酶活性测定方法
肝素如何影响a-l-岩藻糖苷酶测定
纤维素酶的纯化方法
关于脂肪酶分析
α淀粉酶标准
丝氨酸蛋白酶的纯化加不加其抑制剂？
几丁质酶系统交流平台
非特异酯酶染色的办法及试剂
果胶酶活性的测定
对硝基苯酚pNPP测脂肪酶酶活
脂肪酶的测活方法
蛋白酶切的基础问题
外源蛋白酶如何除去
鱼类胰蛋白酶的提取与活性
酯酶同工酶差异性分析实验的聚丙稀酰胺凝胶中需要加SDS吗
配胰蛋白酶
弹性蛋白酶活性测定
肌肉组织的乙酰胆碱酯酶染色法
Folin-酚测蛋白酶活
18与19家族几丁质酶
蛋白酶用法疑问
乙酰胆碱酯酶染色
高产纤维素酶菌株的筛选
木瓜蛋白酶的配制
里氏木霉透明圈筛选纤维素酶
纤维素刚果红培养基筛选纤维素酶产生菌
脂肪酶测定的几个问题
纤维素酶是否包含淀粉酶？
基质蛋白酶活性的测定
脂肪酶固定化
EPS-G7与PNP-G7测α－淀粉酶的区别
胰蛋白酶如何稀释？
如何使菌株产蛋白酶的量提高？
a一醋酸萘酯酶（ANAE)染色法步骤
5-脂氧酶测定方法
如何检测细菌的几丁质酶的活性
3-磷酸甘油醛脱氢酶测定方法及材料
酵母蛋白酶的问题
纤维素酶如何灭菌
蛋白酶抑制剂
胰淀粉酶比总淀粉酶高应该吗
晶状体醛糖还原酶测定方法的具体步骤
肝酯酶的生理功能及其测定方法
脂肪酶活性测定的摩尔消光系数
土壤脱氢酶测定方法
木瓜蛋白酶和胰蛋白酶的区别
什么是抗胆碱酯酶抑制作用
磷酸二酯酶（phosphodiesterase，PDE）的亚型
环糊精柱子用于分离α-淀粉酶
检验科常用的碱性磷酸酶测定方法的精确度
红细胞膜上的乙酰胆碱酯酶的检测
真菌几丁质酶和蛋白酶检测
什么是“胆碱酯酶活性百分数”？
碱性磷酸酯酶标记的蛋白A和IgG二者在elisa中的区别
关于几丁质酶实验的几个问题
蛋白酶解肽段的纯化
关于蛋白酶底物
筛选纤维素酶的实验
淀粉酶测定
牛胰蛋白酶序列
大豆胰蛋白酶抑制剂
纤维素酶的分离纯化方法
胆碱酯酶单位换算
胆碱酯酶
蛋白酶活性的测定方法
关于丝氨酸蛋白酶和弹性蛋白酶的关系
测定微生物的脂肪酶活力时，油脂培养基中的花生油可以用豆油代替吗？
荧光共振能量转移技术的基本原理和应用
酶法分析在果汁检测中的应用
凝乳酶简介
过氧化氢酶的活性测定——高锰酸钾滴定法
DNA的酶切反应心得
纤维素酶的应用进展
功能性内耳毛细胞成功制造为耳聋治疗带来福音
当代最活跃的学科：生命科学与生物学的关系
走进自噬，研究自噬
真核细胞表达系统
如何运输细胞
细胞转染问题
PPARγ基因的序列
苏木素染料的配制
Methylation-Specific Polymerase Chain Reaction
TNFα Promoter Polymorphisms
The Androgen Receptor CAG Repeat and Prostate Cancer Risk
Adenovirus Expressing p53
Gene Expression Analysis Using Filter cDNA Microarrays
lncRNA 通过调控 CD56 参与自然杀伤细胞发育过程
Gene Expression Profiling in Cancer Using cDNA Microarrays
外源基因在真核细胞表达步骤
sf9，空斑试验，滴度测定
Lung Cancer and Exposure to Metals: The Epidemiological Evidence
Epigenetic Markers of Early Tumor Development
Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Anal
Collagen Invasion Assay
用于细胞分析的机器视觉系统简介
Use of Splicing Reporter Minigene Assay to Evaluate the Effect on Splicing of Unclassified Genetic V
G-Rich Oligonucleotides for Cancer Treatment
Construction and Characterization of Minibodies for Imaging and Therapy of Colorectal Carcinomas
软琼脂克隆形成实验
真核生物翻译的调控
d-Glucaric Acid as a Prospective Tumor Marker
Diet, Epigenetics, and Cancer
Interaction of p53 with Cellular Proteins
有限稀释法
Enhanced Permeability and Retention (EPR) Effect for Anticancer Nanomedicine Drug Targeting
Multicolor Spectral Karyotyping in Multiple Myeloma
luciferase检测的标准品问题
吉凯基因分享：结直肠癌研究体内外模型综述
MicroRNA Biogenesis and Cancer
Southern Blotting of IgH Rearrangements in B-Cell Disorders
Human Solid Tumors: Cytogenetic Techniques
细胞组分的分析方法
Assays for Cyclin-Dependent Kinase Inhibitors
单克隆抗体制备-液体石蜡的处理
肿瘤细胞侵袭试验（Tumour Invasion Assay）
A Scientific Basis for Cancer Prevention: Defining the Role of Individual Cytosolic GST Isozyme
Expression Cloning of Human B Cell Immunoglobulins
CV1000活细胞成像分析 HCV 病毒颗粒的内吞作用
稳定转染:从G418浓度确定到单克隆化鉴定
人和哺乳动物原代细胞的蛋白质抽提步骤
Mutational Analysis of the Neurofibromatosis Type 1 Gene in Childhood Myelodysplastic Syndromes Usin
如何得到稳定转染的克隆
TP53 Mutation Detection by SSCP and Sequencing
基因细胞内导入技术
Surface-Epitope Masking (SEM): An Immunological Subtraction Approach for Developing Monoclonal Antib
跨越世纪的生命赞歌（第二部分）
用EBV转染淋巴细胞建立永生化细胞株的方法
细胞房工作守则及注意事项
Telomerase Assay in Renal Cancer
Gold Nanorods: Multifunctional Agents for Cancer Imaging and Therapy
无白血病抑制因子条件下利用SC1（pluripotin）维持小鼠胚胎干细胞的自我更新
Immunohistochemical Localization of TNFα and Its Receptors in the Rodent Central Nervous System
Epigenetics in Myeloid Malignancies
Detection of ras Gene Mutations Using Oligonucleotide Ligation Technology
p53-Induced Gene Expression Analysis
N-Glycosylation Analysis by HPAEC-PAD and Mass Spectrometry
细胞核内显微注射DNA至游离成年哺乳动物神经细胞
原位杂交的难题
电穿孔
Eradication of c-erbB-2-Positive Ovarian Cancer Cells Mediated by Intracellular Expression of Anti-c
昆虫杆状病毒表达系统的研究进展
Single Nucleotide Polymorphism Microarray Analysis of Genetic Alterations in Cancer
无动物成分植物源细胞因子
报告基因检测技术
成人干细胞和再生（第三部分）
CHO细胞表达系统
Bisulfite Pyrosequencing
Tumor Suppression Through Angiogenesis Inhibition
Flow Cytometric Monitoring of Fluorescent Drug Retention and Efflux
Expression and Function of P-Glycoprotein in Normal Tissues: Effect on Pharmacokinetics
5-Fluorouracil Metabolizing Enzymes
Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma
抑制癌细胞生长和血管生成的合成抗体“出世”
科学家首次利用人体干细胞培育出颚骨
Analysis of Glycans on Serum Proteins Using Antibody Microarrays
转染细胞的问题
Analysis of RB Action in DNA Damage Checkpoint Response
FLT3 Mutations in Acute Myeloid Leukemia
动物细胞培养技术平台
Utility of Array Comparative Genomic Hybridization in Cytogenetic Analysis
Use of Tumor-Activated Hepatic Stellate Cell as a Target for the Preclinical Testing of Anti-Angioge
Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells
Clonality Detection of Expanded T-Cell Populations in Patients With Multiple Myeloma
Overcoming Multidrug Resistance in Cancer: Clinical Studies of P-Glycoprotein Inhibitors
Overview of Novel Anticancer Drug Targets
PCR Analysis of Microsatellite Instability
MHC 四聚体助力细胞治疗基础研究：CTL 表位筛选
Human Recombinant Fab Antibodies with T-Cell Receptor-Like Specificities Generated from Phage Displa
Markers for Anti-cytotoxic T-lymphocyte Antigen 4 (CTLA-4) Therapy in Melanoma
Measuring Cyclin-Dependent Kinase Activity
Transgenic Animal Models
Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR
galactosidase assay for cells transfected with pSV- -Galactosidase Vector
Tailoring Kinetics of Antibodies Using Focused Combinatorial Libraries
用LIPO2000转染HepG2细胞的问题
腺病毒能在293中复制吗？
Cytogenetics in Myelodysplastic Syndromes
Detection and Quantification of Leukemia-Specific Rearrangements
细胞因子的检测方法
Idiotype Gene Rescue in Follicular Lymphoma
Clinical Protocol for p53 Gene Therapy for Liver Tumors
Tailoring Natural Effector Functions: Antibody Engineering Beyond Humanization
抑制大肠癌向肝脏转移的新方法
Molecular Characterization of Inflammation-Induced JNK/c-Jun Signaling Pathway in Connection with Tu
Single-Domain VH Antibody Fragments from a Phage Display Library
Galectin-3 Binding and Metastasis
细胞因子和重组蛋白溶解必读(一）
转染细胞不表达怎么办
萤光素酶载体PGL3转染细胞要不要加G418
g418如何配制
细胞接种密度
质体转染293细胞后,细胞漂浮死亡？
Intercellular Adhesion Molecules (ICAM-1, VCAM-1, and LFA-1) in Adenocarcinoma of Lung: Technical As
caspase-3的检测方法
做稳定转染选择何种质粒提取试剂盒
请教 luciferase 的具体操作PROTOCOL
GFP转染后克隆筛选
免疫磁珠分选的问题
细胞稳定转染建系后再转染的问题
如何提高U251的转染效率
免疫荧光双标
Immunohistochemical Analysis of 8-Nitroguanine, A Nitrative DNA Lesion, in Relation to Inflammation-
转染过程
稳定转染细胞株的鉴定
同一种细胞，不同质粒转染，那G418筛选浓度都一样吗
关于CHO-K1细胞稳定转染和瞬转COS的问题
悬浮细胞转染后筛选问题求助
逆转录病毒包装
G418筛选实验相关问题
cos-7的转染
Gene Gun Delivery Systems for Cancer Vaccine Approaches
微胶囊造粒技术在细胞移植，药物和食品研究中的成功应用
细胞凋亡后beta－actin会降解吗？
Clonogenic Cell Survival Assay
SDS -PAGE and Western Blotting Techniques
Sulforhodamine B Assay and Chemosensitivity
The Concept of Multiple-Target Anti-miRNA Antisense Oligonucleotide Technology
Molecular Characterization of Human Papillomaviruses by PCR and In Situ Hybridization
Measuring MDR-1 by Quantitative RT-PCR
The Use of M-FISH and M-BAND to Define Chromosome Abnormalities
Antibody Targeting of Nanoparticles to Tumor-Specific Receptors: Immunoliposomes
荧光法研究啶虫脒与活性污泥胞外聚合物的相互作用取得进展
Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas
光调控细胞功能——新的研究方向
Nonviral Jet-Injection Technology for Intratumoral In Vivo Gene Transfer of Naked DNA
数据的显示与分析
科学家创造出由“细胞”驱动的晶体管
Cell Migration and Invasion Assays
挑蚀斑的方法
Polymer-Based Delivery of RNA-Based Therapeutics in Ovarian Cancer
Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
PCR-Based Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes by GeneScan Analysis or Het
Immunoglobulin Gene Mutation Patterns and Heterogeneity of Marginal Zone Lymphoma
Immunostaining Human Paraffin-Embedded Sections for Mismatch Repair Proteins
pIRES2-eGFP转染细胞的经验
Immunoprecipitation to Determine JAK Kinase Activation in Response to Interleukins in Ovarian Cancer
四唑盐（MTT）比色法
细胞株构建新技术——IOS(Integration Operating System)
Molecular Diagnosis of the 5q Deletion in Malignant Myeloid Disorders
小鼠椎体感光器单细胞吸入记录
用于组织工程研究的水凝胶三维包裹细胞法
甲醇酵母表达注意事项
肾癌的survivin mRNA 基因序列
细胞电融合的原理和方法
荧光激活细胞分选术分离特定细胞群
成人干细胞和再生（第四部分）
正常人胚肺成纤维细胞的培养
Production of Antibody Fragments in a Bioreactor
HSV-tk/IL-2 Gene Therapy for Glioblastoma Multiforme
Electron Microscopic Assessment of Adenovirus-Mediated Transfer
Epigenetics of Colon Cancer
Cationic Liposome Gene Transfer
Sphere-Forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells
跨越世纪的生命赞歌（第三部分）
Cell Migration and the Boyden Chamber
人肝癌细胞与抑癌基因的关系
转染细胞全死了的原因
Saporin Suicide Gene Therapy
Fluorescence In Situ Hybridization on Formalin-Fixed, Paraffin-Embedded Tissue Sections
Preclinical Testing of Antileukemic Drugs Using an In Vivo Model of Systemic Disease
Detection of Mitochondrial Localization of p53
Construction of Semisynthetic Antibody Libraries
茜素红染液的配制
The PFP/RAG2 Double-Knockout Mouse in Metastasis Research: Small-Cell Lung Cancer and Prostate Cance
B7-H Abnormalities in Melanoma and Clinical Relevance
Antibody Techniques Used in the Study of Anaplastic Lymphoma Kinase-Positive ALCL
A Phase II Trial of Intratumoral Injection with a Selectively Replicating Adenovirus (ONYX-015) in P
Tumor-Specific Metastasis to Lung Using Reporter Gene-Tagged Tumor Cells
研究通过转分化获得神经元限制性前体细胞
免疫细胞化学SP法与SABC法的区别
Abrogation of p53-Induced Apoptosis by the Hepatitis B Virus X Gene
美开发出成体细胞转化为干细胞新方法
【生物美学入门课程】如何快速简单地绘制论文插图
美研究用猪膀胱促人体肌肉再生
CHO细胞表达系统存在的问题及前景展望
肿瘤形成的外部化学生物和物理致癌物因素
PNAS：真核生物囊泡转运机理新思路
真核细胞表达的转录水平调控
甲醇酵母系统表达的影响因素
骨髓瘤细胞的体内接种问题
[D-Arg6, D-Trp7,9, NmePhe8]-Substance P (6-11) Activates JNK and Induces Apoptosis in Small Cell Lun
Generation and Screening of a Modular Human scFv Expression Library from Multiple Donors
基于细胞的酶联免疫检测实验
Purification of Primary Malignant B-Cells and Immunoblot Analysis of Bcl-2 Family Proteins
Predictive Mathematical Modeling in Metastasis
Determination of Peak Serum Levels and Immune Response to the Humanized Anti-Ganglioside Antibody-In
miRNA Profiling on High-Throughput OpenArray System
Identification and Assembly of V Genes as Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma
A Nested RT-PCR Assay to Detect BCR/abl
细胞系鉴定你 get 了么？
肝素，硫酸类肝素，低分子肝素的区别
平板细胞克隆形成试验
Analyzing the Regulation and Function of ATM
Chromogenic In Situ Hybridization in Tumor Pathology
CHO细胞表达系统在疫苗研制中的应用
Southern Blotting of Genomic DNA from Lung and Its Tumors: Application to Analysis of Allele Loss on
RT-PCR Quantitation of HSP60 mRNA Expression
Selective Immortalization of Tumor-Specific T Cells to Establish Long-Term T-Cell Lines Maintaining
Phosphatidylinositol Kinase Activity in Ovarian Cancer Cells
华人博士发明单个神经细胞及突触体内标识技术
Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunopre
FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay
Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cell
Simultaneous Humanization and Affinity Optimization of Monoclonal Antibodies
Control of Mitotic Entry After DNA Damage in Drosophila
Apoptotic Caspase Activation and Activity
DNA-Mediated Gene Transfer
Analyzing the Spindle Checkpoint in Yeast and Frogs
Purification of Growth Factor mRNA in Renal Tissues:bFGF-2, FGF-2, TGFα, and EGFR
Flow Cytometric Analysis of p53-Induced Apoptosis
Microdissection of Histologic Sections: Manual and Laser Capture Microdissection Techniques
Isolation of Human Fab Fragments Against Ovarian Carcinoma Using Guided Selection
干细胞和衰老的终结（第四部分）
外源基因在真核细胞表达技术
哺乳动物细胞表达系统及其研究进展
Direct tissue explant method for derivation and study of extravillous trophoblas
Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Us
G418原代细胞的筛选
xCELLigence系统内源性GPCRs细胞功能研究
xCELLigence系统实时检测神经毒性
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models
原代上皮细胞与原代成纤维细胞培养的分离纯化
微砌体技术构建细胞群
PriCells: 原代血管内皮细胞、Angiogenesis、VEGF
正常大鼠原代主动脉平滑肌细胞培养
甲醇酵母表达载体及其元件
NIH3T3 TK-是什么意思
GenBank使用的疑问
CHO细胞与酵母细胞比较
乳糖操纵子（Lac Operon）
跨越世纪的生命赞歌（第一部分）
诱导多能干细胞
Exosomes as a Potential Tool for a Specific Delivery of Functional Molecules
Reversing Agents for ATP-Binding Cassette Drug Transporters
Classification of AML Using a Monoclonal Antibody Microarray
流式细胞术的基本理论
甲醇酵母表达系统的应用及前景
毕式酵母表达常见问题及解析
脾细胞和骨髓瘤细胞的融合
Enzyme Immunoassay of Urinary -Core Fragment of Human Chorionic Gonadotropin as a Tumor Marker for O
蚀斑试验琼脂dmem凝固的解决方案
真核细胞常见表达载体
细胞生长曲线的测定这些问题你遇到过吗？
应用胚胎干细胞衍生内皮细胞治疗后肢缺血
利用gentleMACS Dissociator制备小鼠脾脏单细胞悬液
骨髓造血干细胞归巢
成年心肌细胞的分离、遗传操作和共聚焦成像
成人干细胞和再生（第二部分）
用24孔板做过成骨细胞的钙结节染色计数
细胞-基质粘附实验
fugene做转染的疑问？
悬浮细胞的免疫组化
钙离子浓度变化数据的处理
检测RAS、MAPK、p38、p21
细胞内钙离子浓度测定的问题
启动子片断插入到了pgl3basic载体时未出现荧光的原因
免疫细胞化学固定液的选择
免疫磁珠分选后阳性细胞极少的问题
淋巴细胞分离的关键点
请问淋巴细胞分离液分离的淋巴细胞需要设门吗
血管生成实验的设计
测定物质的亲水性和疏水性问题
葡萄糖转运实验
TGF-Beta，FN酶联免疫（ELISA）试剂盒的选择
钙离子的测定
细胞上清液胰岛素的测定
测定细胞内钙离子浓度的方法
测定NF-κB 活性
如何检测不同细胞siRNA转染效率
MALDI-MS分析
HEK293细胞中是否能查到p53蛋白
ELISA检测中的问题
ELISA测细胞上清肿瘤坏死因子TNF-a
THP1核酸代谢
2009年SCI论文投稿步骤及其经验总结
流式细胞术的应用
Transfection protocol for 6-well dish
Interferon Alpha, GM-CSF-Activated T Cells, and IL-6 in Renal Cell Carcinoma
Breast Tissue Microarrays
Photodynamic Therapy in Lung Cancer: A Review
Quantitation of FISH Signals in Archival Tumors
液体闪烁仪的探测下限
成人干细胞和再生（第五部分）
干细胞和衰老的终结（第二部分）
干细胞和衰老的终结（第一部分）
Ultrasound-Mediated Gene Transfection
Single Cell and Spheroid Collagen Type I Invasion Assay
Assessment of mRNA Splice Variants by qRT-PCR
8 Tumor Models for Evaluation of P450 Gene Therapy In Vivo
Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment
Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination
Strain-Dependent Differences in the Expression of the Oncofetal Protein p65 in Mice Susceptible and
Cross-linking Subtractive Hybridization to Identify Tumor Suppressor Genes
The Use of Denaturing High Performance Liquid Chromatography (DHPLC) for Mutation Scanning of Heredi
In Vitro Functional Study of miR-126 in Leukemia
Silicon Nanowire Biosensor for Ultrasensitive and Label-Free Direct Detection of miRNAs
The Characterization of Chromosomal Abnormalities Using Fluorescence In SituHybridization Procedures
Sequential Fluorescence In Situ Hybridization Analysis for Trisomy 12 in B-Cell Chronic Lymphocytic
Analysis of p21CDKN1A Recruitment to DNA Excision Repair Foci in the UV-Induced DNA Damage Response
Proteolytic Systems: Constructing Degradomes
Magnetic Aerosol Targeting of Nanoparticles to Cancer: Nanomagnetosols
High-Throughput Analysis of Serum Antigens Using Sandwich ELISAs on Microarrays
Polymerase Chain Reaction Detection of DNA Sequence Deletions
Multimodal Nanoparticulate Bioimaging Contrast Agents
Immunoglobulin Fusion Proteins as a Tool for Evaluation of T-Cell Costimulatory Molecules
Differential Display Techniques to Identify Tumor Suppressor Gene Pathways
Detection of ErbB Receptor Family Tyrosyl Phosphorylation in Ovarian Carcinoma Cells
The 5T2MM Murine Model of Multiple Myeloma: Maintenance and Analysis
Measurement of Microvessel Density in Primary Tumors
Introduction to the Use of Regression Models in Epidemiology
The Immunohistochemical Method
Ultrasound-Guided Intracardial Injection and In Vivo Magnetic Resonance Imaging of Single Cells in M
Statistical Design and Evaluation of Biomarker Studies
PKR in Innate Immunity, Cancer, and Viral Oncolysis
Magnetic Resonance Imaging of Tumor Response to Chemotherapy
TNF Signaling: Key Protocols
In Vitro Testing of Chemosensitivity in Physiological Hypoxia
Role of Tumor Suppressors in DNA Damage Response
Expression Profiling of Lung Cancer Based on Suppression Subtraction Hybridization (SSH)
Synthesis, Characterization, and Functionalization of Gold Nanoparticles for Cancer Imaging
Mutational Analysis of the Androgen Receptor Using Laser Capture Microdissection and Direct Sequenci
Assessment of Resistance to Anoikis in Ovarian Cancer
Detection of BCR-ABL in Hematological Malignancies by RT-PCR
T-Cell Receptor Molecular Diagnosis of T-Cell Lymphoma
Assembling a Tumor Progression Model
Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements
Targeted Delivery of Expression Plasmids to the Lung via Macroaggregated Polyethylenimine-Albumin Co
Epigenetic Regulation of MicroRNA Expression in Cancer
Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/In Situ Assay
Assessing Expression of Apoptotic Markers Using Large Cohort Tissue Microarrays
Quantitative Analysis of PRAME for Detection of Minimal Residual Disease in Leukemia
Silencing Epidermal Growth Factor Receptor by RNA Interference in Glioma
Novel Insights/Translational Implication from the Emerging Biology of Melanoma
Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, an
Matrix Metalloproteinase Expression in Lung Cancer
Hereditary Breast and Ovarian Cancer Syndrome: The Impact of Race on Uptake of Genetic Counseling an
Metabolic Monitoring of Chemosensitivity with 18FDG PET
Studying Drug Resistance Using Genetically Engineered Mouse Models for Breast Cancer
Application of Silicon Sensor Technologies to Tumor Tissue In Vitro: Detection of Metabolic Correlat
Detection of Chromosome Abnormalities in Leukemia Using Fluorescence In Situ Hybridization
Choline Metabolic Profiling by Magnetic Resonance Spectroscopy
Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma
Comparative Multiplex PCR and Allele-Specific Expression Analysis in Human Lung Cancer: Tools to Fac
Tumor Deprivation of Oxygen and Tumor Suppressor Gene Function
Antisense Oligonucleotides Targeting RI Subunit of Protein Kinase A: In Vitro and In Vivo Anti-Tumor
Proteomic Profiling of Ovarian Cancer Models Using TMT-LC-MS/MS
Immunohistochemical Detection of Ornithine Decarboxylase as a Measure of Chemosensitivity Testing
9 Intracellular Single-Chain Antibodies for Gene Therapy
Multiple Labeling Techniques for Fluorescence Microscopy
On-Demand Cleavable Linkers for Radioimmunotherapy
Intratumoral Gene Transfer of the Cytosine Deaminase Gene for the Treatment of Breast Cancer
Screening for miRNA Expression Changes Using Quantitative PCR (Q-PCR)
Genetic Epidemiology Studies in Hereditary Non-Polyposis Colorectal Cancer
Detection of Telomerase Enzyme Activity by TRAP Assay
Ex Vivo Cytokine Gene Transfer in Melanomas by Using Particle Bombardment
YKL-40: A Novel Marker Shared by Chronic Inflammation and Oncogenic Transformation
Fluorescence In Situ Hybridization (FISH) to Metaphase and Interphase Chromosomes
In Situ Hybridization of Cells and Tissue Sections
TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s)
Molecular Cytogenetic Applications in Analysis of the Cancer Genome
Analysis of the TLR/NF-B Pathway in Antigen-Presenting Cells in Malignancies Promoted by Inflammatio
Conversion Technology and Cancer Predispositions
PARP Cleavage and Caspase Activity to Assess Chemosensitivity
Molecular Mechanism of ATP-Dependent Solute Transport by Multidrug Resistance-Associated Protein 1
Chick Heart Invasion Assay
Statistical Methods in Cancer Epidemiological Studies
Flavonoid Compounds in the Prevention and Treatment of Prostate Cancer
The Role of MicroRNAs in the Management of Liver Cancer
Pathological Staging of Melanoma
Flow Cytometric Analysis of Cell Cycle Control by Tumor Suppressor Genes
Selecting Patients for KIT Inhibition in Melanoma
Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR
Myocardial Infarction: Animal Models
Single Nucleotide Polymorphisms in DNA Repair Genes and Prostate Cancer Risk
Approaches to Determine Expression of Inflammatory Cytokines
Gene Therapy for Treatment of Brain Tumors (HSV-tKIn Vivo Gene Transfer): A Case Study
In Vivo Xenograft Models of Breast Cancer Metastasis
Monitoring Methylation and Gene Expression in Cancer
20Telomere Length Changes in Human Cancer
Microarrays for Protease Detection in Tissues and Cells
Identifying the Origin and Phenotype of Cells in Tumor Xenografts
Application of Proteomics in Cancer Gene Profiling: Two-Dimensional Difference in Gel Electrophoresi
High-Throughput RNA Sequencing in B-Cell Lymphomas
Serial Biopsies/Fine-Needle Aspirates and Their Assessment
Histoculture Drug Response Assay to Monitor Chemoresponse
HSV-1 Vectors for Gene Therapy of Experimental CNS Tumors
Growth of Prostatic Epithelial and Stromal Cells In Vitro
Determination of Cancer Allelotype
Mutation Analysis of Large Genomic Regions in Tumor DNA Using Single-Strand Conformation Polymorphis
Induction of Programmed Cell Death with an Antisense Bcl-2 Oligonucleotide
Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma
Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic C
High-Sensitivity Cytotoxicity Assays for Nonadherent Cells
Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells
Yeast Two-Hybrid Screening as a Means of Deciphering Tumor Suppressor Pathways
Adoptive Immunotherapy
Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity
Green Fluorescent Protein Retroviral Vector: Generation of High-Titer Producer Cells and Virus Super
Utilizing Saccharomyces Cerevisiae to Identify Aneuploidy and Cancer Susceptibility Genes
Isolation and Culture of Melanoma and Naevus Cells and Cell Lines
DNA Methylation in Pancreatic Cancer: Protocols for the Isolation of DNA and Bisulfite Modification
Analysis of Genome-Wide DNA Methylation Profiles by BeadChip Technology
Panhandle PCR Approaches to Cloning MLL Genomic Breakpoint Junctions and Fusion Transcript Sequences
DNA Methylation in Promoter Region as Biomarkers in Prostate Cancer
Digital Candidate Gene Approach (DigiCGA) for Identification of Cancer Genes
Microdissection and Extraction of DNA From Archival Tissue
Isolation of Inflammatory Cells from Human Tumours
Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth
Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography
Utility of Fine-Needle Aspiration Biopsy for Prospective Analysis of Patients Undergoing Therapy for
Conformation-Sensitive Gel Electrophoresis for Detecting BRCA1 Mutations
Cancer Gene Therapy With Heat Shock Protein-65 Gene
Angiogenesis, Metastasis, and Lung Cancer: An Overview
Karyotyping Lymph Node Biopsies in Non-Hodgkins Lymphoma
IGF-1 Antisense Strategies for Cancer Treatment
Tissue Microarrays as a Tool in the Discovery and Validation of Tumor Markers
Breast Cancer and the Role of Exercise in Women
Genome-Wide Determination of DNA Methylation by Hpa II Tiny Fragment Enrichment by Ligation-Mediated
In Vitro Models of Early Neoplastic Transformation of Human Mammary Epithelial Cells
Phosphotyrosine Phosphatase Activity in Ovarian Carcinoma Cells: Stimulation by GnRH in Plasma Membr
Inflammation-Related Aberrant Patterns of DNA Methylation: Detection and Role in Epigenetic Deregula
Chemoprevention of Lung Cancer
Tumor Suppressor Gene Therapy
Identification of Human Pancreatic Cancer Stem Cells
Conventional Cytogenetic Techniques in the Diagnosis of Childhood Acute Lymphoblastic Leukemia
Identification of Steroid Hormone-Regulated Genes in Breast Cancer
Surgical Techniques for Treatment of Primary Cutaneous Melanoma
In Situ Hybridization Combined With Immunohistochemistry to Localize Gene Expression
Contribution of Alcohol and Tobacco Use in Gastrointestinal Cancer Development
Aberrant Crypt Foci in Colon Cancer Epidemiology
In Vitro Invasion Assay Using Matrigel: A Reconstituted Basement Membrane Preparation
Defining Cancer Stem Cells by Xenotransplantation in Zebrafish
Molecular Alterations in Lung Cancer: Impact on Prognosis
Clinical Development of Antisense Oligonucleotides as Anti-Cancer Therapeutics
Molecular Characterization of Melanoma-Derived Antigens
Chemosensitization of Tumor Cells: Inactivation of Nuclear Factor-Kappa B Associated with Chemosensi
Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes
Risk Factors and Gene Expression in Esophageal Cancer
Discovery of Antibody Biomarkers Using Protein Microarrays of Tumor Antigens Cloned in High Throughp
Phosphatidylinositol 4-Kinase Assay in Ovarian Carcinoma Cells
Clinically Accurate Orthotopic Mouse Models of Cancer
Cancer Cohort Consortium Approach: Cancer Epidemiology in Immunosuppressed Groups
Epidemiology of Stomach Cancer
In Vivo Footprinting and DNA Affinity Chromatography for Analysis of p53 DNA Binding Ability
Cytogenetic Studies Using FISH: Background
Fluorescence In Situ Hybridization: Application in Cancer Research and Clinical Diagnostics
Inflammatory Bowel Disease: A Model of Chronic Inflammation-Induced Cancer
Detection of Telomerase hTERT Gene Expression and Its Splice Variants by RT-PCR
The Use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells
Mining Expressed Sequence Tag (EST) Libraries for Cancer-Associated Genes
Assays for HSP90 and Inhibitors
Regulatory Aspects for Translating Gene Therapy Research into the Clinic
99mTc-Annexin A5 Uptake and Imaging to Monitor Chemosensitivity
Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas
Frozen Biopsy Collection and Storage: Frozen Biopsy Samples
Chromosomal Copy Number Analysis in Melanoma Diagnostics
Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer Cells
DNA Methylation Changes in Prostate Cancer
Detection of MicroRNAs in Cultured Cells and Paraffin-Embedded Tissue Specimens by In Situ Hybridiza
2-DE-Based Proteomics for the Analysis of Metastasis-Associated Proteins
Targeting the Mitochondria to Enhance Tumor Suppression
High-Throughput Technology: Green Fluorescent Protein to Monitor Cell Death
A High-Throughput Methodology for Identifying Molecular Targets Overexpressed in Lung Cancers
Assessment of Insulin-Like Growth Factors and Mutagen Sensitivity as Predictors of Lung Cancer Risk
Some Difficult Choices in Cytogenetics
Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo
Animal Models of Endotoxic Shock
Coculture of Prostate Cancer Cells
Multiple Myeloma: Challenges and Opportunities
Construction and Characterization of RNase-Based Targeted Therapeutics
The Effects of Butyrate and the Role of c-myc in N.1 Ovarian Carcinoma Cells Determined by Northern
RNase Protection Assay Analysis of mRNA for TGF1-3 in Ovarian Tumors
Serum and Tissue Biomarkers in the Prognosis and Treatment of Breast Cancer
The rapid progress in understanding the molecular biology of cancer cells has made a large impact on
Recombinant Single-Chain and Disulfide-Stabilized Fv Immunotoxins for Cancer Therapy
Bioactive Interleukin-6 Levels in Serum and Ascites as a Prognostic Factor in Patients with Epitheli
Basic Principles for the Study of Metastasis Using Animal Models
RFLP Molecular Analysis of the Urokinase-Type Plasminogen Activator Gene
ERBB4 Mutation Analysis: Emerging Molecular Target for Melanoma Treatment
Histopathological Assessment of Metastasis
Methods for Use of Peptide-MHCTetramers in Tumor Immunology
溶解方法及溶剂问题
Lectin Array-Based Strategies for Identifying Metastasis-Associated Changes in Glycosylation
Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal
Interaction Between the Retinoblastoma Protein and Protein Phosphatase 1 During the Cell Cycle
Correlation of Caspase Activity and In Vitro Chemo-Response in Epithelial Ovarian Cancer Cell Lines
Measuring DNA Adducts by Immunoassay (ELISA)
Detection of Telomerase Activity in Patients with Pancreatic Cancer
Cancer Occurrence
COLD-PCR Enriches Low-Level Variant DNA Sequences and Increases the Sensitivity of Genetic Testing
SAGE as a Strategy to Isolate Cancer-Related Genes
Models of Hormone Resistance In Vitro and In Vivo
Evaluation of Environmental and Personal Susceptibility Characteristics That Modify Genetic Risks
Angiogenesis Assays
Antisense Oligonucleotides as Research Tools
Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues
Apoptosis: A Technical Overview
Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH)
Determination of the Catalytic Activities of mTOR and Other Members of the Phosphoinositide-3-Kinase
Signal Transduction: A Technical Overview
Real-Time RT-PCR (Taqman) of Tumor mRNA to Predict Sensitivity of Specimens to 5-Fluorouracil
Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach
Identification and Characterization of Hematopoietic Stem and Progenitor Cell Populations in Mouse B
Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate
RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molec
Lymphatic Invasion as a Prognostic Biomarker in Primary Cutaneous Melanoma
Kinetworks Protein Kinase Multiblot Analysis
Organizational Issues in Providing High-Quality Human Tissues and Clinical Information for the Suppo
Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells
The Hollow Fiber Assay
Determinants of Incidence of Primary Fallopian Tube Carcinoma (PFTC)
以细胞为靶目标的噬菌体肽筛选材料与方法
Flow Cytometric Evaluation of Multidrug Resistance Proteins
Clinical, Pathologic, and Imaging Features and Biological Markers of Uveal Melanoma
Xenografting Human Colon Cancers
Immune System Functional Pathway Analysis Using Single Cell Network Profiling (SCNP): A Novel Tool i
Detection of Telomerase Activity in Lung Cancer Tissues
Assays for Raf-1 Kinase Phosphorylation and Activity in Human Small Cell Lung Cancer Cells
Selection and Development of Biomarkers for Bladder Cancer
Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis
Metaphase Cytogenetic Techniques in Multiple Myeloma
Differential Display to Define Molecular Markers and Genes That Mediate Malignancy
Therapeutic Strategies Using Inhibitors of Angiogenesis
Blocking Survivin to Kill Cancer Cells
Transgenic Mouse Models for Prostate Cancer: Identification of an Androgen-Dependent Promoter and Cr
HER2 FISH in Breast Cancer
Myeloperoxidase Promoter Region Polymorphism and Lung Cancer Risk
7 In Vitro Methods for Evaluation of P450-Based Anticancer Gene Therapy
Targeted Alpha Therapy of Prostate Cancer
Binding Assay for Selectins
Analysis of Alterations in a Base-Excision Repair Gene in Lung Cancer
The SCID-hu Myeloma Model
Cyclooxygenase 2-Dependent Regulation of Antitumor Immunity in Lung Cancer
Markers of Tumor Burden: An Overview
17 Direct Sequencing for Peutz-Jeghers Gene LKB1 (STK11) Mutations
Characterization of Signalling Pathways by Reverse Phase Protein Arrays
Targets in Apoptosis Signaling: Promise of Selective Anticancer Therapy
Approaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells
Analysis of the Mouse Placenta as a Hematopoietic Stem Cell Niche
MicroRNA Regulation of Growth Factor Receptor Signaling in Human Cancer Cells
SPR1: An Early Molecular Marker for Bronchial Carcinogenesis
Gold Nanocages for Cancer Imaging and Therapy
Proteomic Evaluation of Cancer Cells: Identification of Cell Surface Proteins
Flow Cytometric Analysis of Tumor Suppressor Gene-Induced Apoptosis
Applications of Gold Nanorods for Cancer Imaging and Photothermal Therapy
Immunohistochemical Detection and Quantitation of Cell Surface Receptors for Prostanoids
Principles of Cancer Cell Culture
Advantages and Disadvantages of Multiple Different Methods of Adenoviral Vector Construction
EWS Gene Fusions as Diagnostic Markers in Sarcomas: Principles and Guidelines
Studies on Androgen Receptor Mutations and Amplification in Human Prostate Cancer
Cellular Predictive Factors of Drug Resistance in Non-Small Cell Lung Carcinomas
p53Transfectants in Ovarian Cancer
The Guideline of the Design and Validation of MiRNA Mimics
Optical Proteolytic Beacons for In Vivo Detection of Matrix Metalloproteinase Activity
In Vivo Vascular Leakage Assay
The Identification of Colon Cancer Susceptibility Genes by Using Genome-Wide Scans
Immunocompetent Mouse Model of Ovarian Cancer for In Vivo Imaging
Stability and Ubiquitination of the Tumor Suppressor Protein p53
The Clinical Use of PET/CT in the Evaluation of Melanoma
Adhesion of Tumor Cells to Matrices and Endothelium
Representational Difference Analysis of Gene Expression
Signal Transduction Study Using Gene-Targeted Embryonic Stem Cells
Characterization of Nonmalignant and Malignant Prostatic Stem/Progenitor Cells by Hoechst Side Popul
Designing Plasmid Vectors
Immunohistochemistry and In Situ Hybridization in Pancreatic Neoplasia
Identification of Senescence in Cancer Cells
Cell Aggregation Assays
What Is Cancer Nanotechnology
Analysis of Cyclin-Dependent Kinase Activity
Use of Nucleotide Excision Repair-Deficient Mice as a Model For Chemically Induced Lung Cancer
Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia
Prostate Epithelial Stem Cell Isolation and Culture
Chemotherapy-Inducible Vector for Gene Therapy of Cancer
The NF-B Transcription Factor Pathway as a Therapeutic Target in Cancer: Methods for Detection of NF
Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR
Racial Differences in Clinical Outcome After Prostate Cancer Treatment
Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues
In Vivo Models of Human Prostate Cancer Bone Metastasis
Thriving for Clues in Variations Seen in Mortality and Incidence of Cancer: Geographic Patterns, Tem
Spontaneous and Experimental Metastasis Models: Nude Mice
Analysis of Nuclear and Cytoplasmic Degradation of p53 in Cells after Stress
The Epidemiology of Ovarian Cancer
Genetic Analysis Using Microarrays
Computer Simulation of the Metastatic Progression
Chromosome Preparations from Bone Marrow in Acute Lymphoblastic Leukemia: Cytogenetic Techniques
Chemoprotection by Transfer of Resistance Genes
In Vitro Invasion Assay Using Matrigel
Familial Ovarian Cancer
Green Fluorescent Protein for Metastasis Research
Utilization of Thyroid Transcription Factor-1 Immunostaining in the Diagnosis of Lung Tumors
Dendritic Cell/Myeloma Hybrid Vaccine
Cellular Chemosensitivity Assays: An Overview
Representational Difference Analysis as a Tool in the Search for New Tumor Suppressor Genes
Role of Receptor Tyrosine Kinases in Lung Cancer
Purification of Annexin V and Its Use in the Detection of Apoptotic Cells
Modulation of MicroRNAs for Potential Cancer Therapeutics
A Molecular Technique Useful in the Detection of Occult Metastases in Patients with Melanoma: RT-PCR
Sequencing of the Tumor Suppressor Gene TP 53
Advances in Adjuvant Therapy: Potential for Prognostic and Predictive Biomarkers
Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inv
Role of Xenobiotic Metabolic Enzymes in Cancer Epidemiology
Altered Surface Markers in Lung Cancer: Lack of Cell-Surface Fas/APO-1 Expression in Pulmonary Adeno
Construction and Analysis of Multiparameter Prognostic Models for Melanoma Outcome
Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems
Analysis of Tumor-Associated Mucin Glycotopes by Western Transfer Methods
Use of Minicircle Plasmids for Gene Therapy
Combined Adenoviral Transfer of Tumor Suppressor and Cell-Cycle Genes for Tumor-Cell Apoptosis
Interphase Cytogenetics in Paraffin-Embedded Ovarian Tissue
Polymeric Micelles: Polyethylene Glycol-Phosphatidylethanolamine (PEG-PE)-Based Micelles as an Examp
Small-Cell Lung Cancer (SCLC) Cell Adhesion on E- and P-Selectin Under Physiological Flow Conditions
Analysis of Apoptosis in Tissue Sections
High-Resolution Quantitative Methylation Analysis of MicroRNA Genes Using Pyrosequencing
The Application of Differential Display as a Gene Profiling Tool
Detection of Differentially Expressed Genes in Cancer Using Differential Display
DNA Methylation Changes in Cervical Cancers
Complete Open Reading Frame (C-ORF) Technique: Rapid and Efficient Method for Obtaining Complete Pro
A Xenopus Cell-Free System for Analysis of the Chfr Ubiquitin Ligase Involved in Control of Mitotic
Detection of Chondroitin Sulfate Proteoglycan 4 (CSPG4) in Melanoma
Isolation of Novel Markers for Hepatocellular Carcinoma by a Subtraction-Enhanced Display Technique
Induction of c-fos Gene Expression by Urokinase-Type Plasminogen Activator in Human Ovarian Cancer C
Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma
Antisense IGF-I for Hepatocellular Carcinoma
Purification of the Mitotic Checkpoint Complex, an Inhibitor of the APC/C From HeLa Cells
PPAR/ Agonist Increases the Expression of PGE2 Receptor Subtype EP4 in Human Lung Carcinoma Cells
Methods to Analyze the Effects of the Urokinase System on Cancer Cell Adhesion, Proliferation, Migra
Regulation of Angiostatin Mobilization by Tumor-Derived Matrix Metalloproteinase-2
Intratumoral Gene Transfer of the HLA-B7 Gene Into Colon Carcinoma Metastases
Identification of Tumor-Associated Antigens as Diagnostic and Predictive Biomarkers in Cancer
Detection of Low Levels of DNA Fragmentation in Ovarian Tumor Cell Lines
Polymerase Chain Reaction Based Methods for Assessing Chimerism Following Allogeneic Bone Marrow Tra
Genetic Modification of Cell Lines to Enhance Their Metastatic Capability
Tumor Necrosis Factor as a Pharmacological Target
Practical Methods for Tissue Microarray Construction
Quantitative, Competitive RT-PCR Analysis of Biomarkers in the Study of Neoplasia
Detection of Chromosomal Aberrations in Lung Tissue and Peripheral Blood Lymphocytes Using Interphas
Tumor-Targeted Salmonella: Strain Development and Expression of the HSV-tK Effector Gene
Use of Diphenylamine in the Detection of Apoptosis
Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-
Isolation and Culture of Renal Cancer Cell Lines
Prognostic Relevance of Angiogenic, Proliferative, and Apoptotic Factors in Lung Carcinomas: A Case
Immunohistochemical Diagnostic and Prognostic Markers for Melanoma
Transfection and DNA-Mediated Gene Transfer
Genetic Engineering in the Mouse: Tuning TNF/TNFR Expression
Polymeric Nanoparticles for Drug Delivery
Collagen-Induced Arthritis as a Model for Rheumatoid Arthritis
Determination of Cell-Specific Receptor Binding Using a Combination of Immunohistochemistry and In V
PEGylated Nanocarriers for Systemic Delivery
Imaging Metastatic Cell Trafficking at the Cellular Level In Vivo with Fluorescent Proteins
Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene
Transforming Growth Factor-1 as a Novel Marker of Response to Therapy for Renal Cell Carcinoma
Quantitation of RNA by Ribonuclease Protection Assay
PCR Diagnosis of T-Cell Lymphoma in Paraffin-Embedded Bone Marrow Biopsies
Parental Smoking and Childhood Leukemia
Retroviral/Lentiviral Transduction and Transformation Assay
Neoadjuvant Endocrine Therapy Models
Immunizing Potential of Cytokine-Transduced Tumor Cells
Clinical and Biological Relevance of Recently Defined Categories of Pulmonary Neoplasia
Ovarian Cancer Genome
Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions
Molecular Characterization of STAT Signaling in Inflammation and Tumorigenesis
Flow Cytometry Enumeration of Apoptotic Cancer Cells by Apoptotic Rate
Functional Analysis of CDK Inhibitor p21WAF1
Essential Techniques of Cancer Cell Culture
Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage
Vascular Endothelial Growth Factor Expression in Non-Small Cell Lung Cancer
Imageable Clinically Relevant Mouse Models of Metastasis
Identifying Associations Between Genomic Alterations in Tumors
Ligand-Coupled Lipoprotein for Ovarian Cancer-Specific Drug Delivery
Automated Fluorescent Differential Display for Cancer Gene Profiling
Electroporative Gene Transfer
MRI and MRS of Human Brain Tumors
Phenotypic and Functional Differences of Dendritic Cells Generated Under Different In Vitro Conditio
cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-B Related Genes in Squamous Cell Carcin
Animal Models of Prostate Cancer
Isolation of Cells Entering Different Programmed Cell Death Pathways Using a Discontinuous Percoll G
p53 Plasmid Preparation and Techniques for Analysis of Gene Transfer and Expression
Screening of Mutations in the ras Family of Oncogenes by Polymerase Chain Reaction-Based Ligase Chai
Origin and Pathogenesis of B Cell Lymphomas
Direct DNA Injection (p53) into HCC Tumors
Detection of HER2 Gene Amplification by Fluorescence In Situ Hybridization in Breast Cancer
Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression
Alterations in Oncogenes, Tumor Suppressor Genes, and Growth Factors Associated with Epithelial Ovar
SSCP and Sequence Analysis of p53 Mutations in Ovarian Tumors
Analysis of Tumor Suppressor Gene-Induced Senescence
Epigenome and DNA Methylation in Oral Squamous Cell Carcinoma
Drug Ratio-Dependent Antagonism: A New Category of Multidrug Resistance and Strategies for Its Circu
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
All-Liquid Separations, Protein Microarrays, and Mass Spectrometry to Interrogate Serum Proteomes: A
23 Assessment of T-Cell Immune Dysfunction in Patients with Renal Cell Carcinoma
Photoaffinity Detection of cAMP Binding Proteins in Ovarian Cancer
Diagnostic Markers in Hepatocellular Carcinoma Using Immunohistochemical Techniques
The Use of Matrigel in the Establishment of Ovarian Carcinoma Cell Lines as Xenografts
Isolation of RNA and DNA From Leukocytes and cDNA Synthesis
Inhibition of Cell Growth by Antisense Oligonucleotides Targeting the Growth-Related Protein Kinase
FISH, CGH, and SKY in the Diagnosis of Childhood Acute Lymphoblastic Leukemia
Delineating Protease Functions During Cancer Development
Background, Methods, and Protocols for the Histopathological Diagnosis of Prostate Carcinoma
Static and Flow Cytometry
Dendritic Cell Vaccines for Cancer Stem Cells
An Overview of Chemosensitivity Testing
In Vitro and In Vivo Assays for the Proliferative and Vascular Permeabilization Activities of Vascul
Assays for In Vitro and In Vivo Synergy
The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies
Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophi
Analysis of Drug Interactions
Subcloning of Ovarian Cancer Cell Lines
Breast Cancer in Asia
Spheroids of Prostate Tumor Cell Lines
High-Throughput and Reliable Protocols for Animal MicroRNA Library Cloning
Nude Mouse Lung Metastases Models of Osteosarcoma and Ewing s Sarcoma for Evaluating New Therapeutic
Chromatin Immunoprecipitation (ChIP) for Analysis of Histone Modifications and Chromatin-Associated
Construction of P450-Expressing Tumor Cell Lines Using Retroviruses
Acquired Risk Factors for Colorectal Cancer
Antiangiogenic Approaches to Renal Cell Carcinoma: Assays for Inhibitors and Clinical Trials
Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Mal
Applications of Tissue Microdissection in Molecular Pathology: Principles and Guidelines
Sensitive Assays for Detection of Lung Cancer: Molecular Markers in Blood Samples
Sentinel Lymph Node Biopsy in Breast Cancer
Cancer Registry Databases: An Overview of Techniques of Statistical Analysis and Impact on Cancer Ep
Culture and Immortalization of Pancreatic Ductal Epithelial Cells
Pooled shRNA Screenings: Experimental Approach
Methods and Approaches in Using Secondary Data Sources to Study Race and Ethnicity Factors
Suppression Subtractive Hybridization for Identification and Functional Analysis of Tumor Suppressor
Gene Immunotherapy for Non-Small Cell Lung Cancer
Detection of MetastaticTumor Cells in Blood by Reverse Transcriptase-Polymerase Chain Reaction
Cytokine Multiplex Analysis
The APC Tumor Suppressor Pathway
Chromosome Microdissection for Detection of Subchromosomal Alterations by FISH
Epidemiology, Pathology, and Genetics of Prostate Cancer Among African Americans Compared with Other
Analysis of Targets and Functions Coregulated by MicroRNAs
Prognostic and Predictive Factors
Neuroendocrine Phenotype of Small Cell Lung Cancer
Current Status of Diagnostic and Prognostic Markers in Melanoma
Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay
Multiplex Caspase Activity and Cytotoxicity Assays
Mixture-Based Peptide Libraries for Identifying Protease Cleavage Motifs
Computational Identification of Cancer Susceptibility Loci
Recent Insights into Drug Resistance in Ovarian Cancer
Dideoxy DNA sequencing is routinely used in research and, increasingly, in clinical care for the det
DNA Sequencing of Cancer-Related Genes for Biomarker Discovery
Biochemical Analysis of the Cell Cycle and Cell Cycle Checkpoints in Transiently Transfected Cells A
The MTT Assay to Evaluate Chemosensitivity
Polymorphisms of DNA Repair Genes: ADPRT, XRCC1, and XPD and Cancer Risk in Genetic Epidemiology
Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer
Differential Expression of Apoptosis-Associated Genes bax and bcl-2 in Ovarian Cancer
Gene Therapy in Melanoma
Chemopreventive Therapeutics: Inhalation Therapies for Lung Cancer and Bronchial Premalignancy
Detecting and Modulating the NF-kB Activity in Human Immune Cells: Generation of Human Cell Lines wi
Molecular Genetic Analysis of Flow-Sorted and Microdissected Ovarian Tumor Cells Improved Detection
Different Study Designs in the Epidemiology of Cancer: Case-Control vs. Cohort Studies
Identification of Mutations in the Retinoblastoma Gene
Isolation of Melanoma Cell Subpopulations Using Negative Selection
WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes
Induction of Tumor Cell Apoptosis by TRAIL Gene Therapy
Topoisomerase I and II Activity Assays
Fluorescence itIn Situ Hybridization
Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer
Mitochondria Potential, Bax Activation, and Programmed Cell Death
Investigating Macrophage and Malignant Cell Interactions In Vitro
Identification of Protein Interaction Partners by the Yeast Two-Hybrid System
Immunity of Lentiviral Vector-Modified Dendritic Cells
Cell Cycle and Drug Sensitivity
Development of Orthotopic Pancreatic Tumor Mouse Models
Analysis of Cell Cycle by Flow Cytometry
Fluorescence In Situ Hybridization for BCR-ABL
Measurement of Ceramide and Sphingolipid Metabolism in Tumors: Potential Modulation of Chemotherapy
Isolation of Tumor Suppressor Genes in Melanoma by cDNA Microarray
The Oncofetal Protein p65 in Breast Cancer Detection
Development of a Cell-Based Assay to Quantify the Inflammatory Potential of Test Substances and Scre
Recombinant Vaccinia Virus MVA for Generation and Analysis of T Cell Responses Against Tumor Associa
Screening for Molecular Markers of Cervical Papillomavirus Infection: Overview of Methods and Their
Cell Motility, Adhesion, Homing, and Migration Assays in the Studies of Tyrosine Kinases
LHRH-Targeted Nanoparticles for Cancer Therapeutics
Mutational Analysis of the Wilms' Tumor (WTI) Gene
Hormonal and Differentiation Agents in Cancer Growth Suppression
Ovarian Cancer Models: Technical Review
A Decade of Cancer Gene Profiling: From Molecular Portraits to Molecular Function
Introduction to the Analysis of the Human G-Banded Karyotype
Wilms Tumor as a Model for Cancer Biology
Tumor Suppressor Gene-Inducible Cell Lines
Cytogenetic Analysis of Cancer Cell Lines
Markers of Apoptosis
p53 Adenovirus as Gene Therapy for Ovarian Cancer
Colony Growth Suppression by Tumor Suppressor Genes
Serological Analysis of Expression cDNA Libraries (SEREX): An Immunoscreening Technique for Identify
Model Systems for Examining Effects of Leukemia Associated Oncogenes in Primary Human CD34+ Cells vi
Gene Transfer into the Colonic Mucosa
mRNA Analyses: A Technical Overview
Magnetic Resonance Spectroscopy of Living Tissues
Cytogenetics of Ovarian Cancer Technical Overview
Establishment of Ovarian Cancer Cell Lines
Preparation of DNA-Protein Complexes Suitable for Spectroscopic Analysis
Enhancer Trap Method Using a Green Fluorescent Protein Reporter Plasmid for Cloning Tissue-Specific
Designing Adenoviral Vectors for Tumor-Specific Targeting
Measurement of Protein Expression A Technical Overview
Detection of Mismatch Repair Gene Expression in Urologic Malignancies
Tumor Marker Discovery by Expression Profiling RNA from Formalin Fixed Paraffin Embedded Tissues
Nanoshells for Photothermal Cancer Therapy
Metabolomic Profiling of Ovarian Carcinomas Using Mass Spectrometry
Aberrant Crypt Foci System to Study Cancer Preventive Agents in the Colon: Principles and Guidelines
Examination of CYP3A and P-Glycoprotein-Mediated DrugDrug Interactions Using Animal Models
DNA Footprinting
Progression Model of Prostate Cancer
Search for and Identification of Novel Tumor-Associated Autoantigens
Induction of Lung Cancer by Passive Smoking in an Animal Model System
Diphenylamine Assay of DNA Fragmentation for Chemosensitivity Testing
Intratumoral Therapy with Cytokine Gene-Modified Dendritic Cells in Murine Lung Cancer Models
Microarray-Based CGH in Cancer
Large Scale Production of Recombinant Antibodies by Utilizing Cellulose-Binding Domains
Methodologies in Assaying Prostate Cancer Stem Cells
In Vitro Invasion Assays
Dissecting the Urokinase Activation Pathway Using Urokinase-Activated Anthrax Toxin
Comparative Genomic Hybridization Analysis
Gene Therapy Vectors Harboring AFP Regulatory Sequences: Preparation of an Adenoviral Vector
Bcl-2 Family Immunohistochemistry
Comparative Genomic Hybridization Technique
Novel Effective Tumor Vaccines for Hepatocellular Carcinoma
Genome-Wide DNA Methylation Profiling: The mDIP-Chip Technology
Hybrid Capture of Putative Tumor Suppressor Genes
Zoom-In Array Comparative Genomic Hybridization (aCGH) to Detect Germline Rearrangements in Cancer S
Analysis of the Spindle-Assembly Checkpoint in HeLa Cells
Assessing Matrix Metalloproteinase Expression and Activity in Hepatocellular Carcinomas
Glycoproteomic Analysis by Two-Dimensional Electrophoresis
Application of Recombinant Antibodies in Cancer Patients
Fluorescence In Situ Hybridization for Cancer-Related Studies
Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour Cells
Somatic Cell Knockouts of Tumor Suppressor Genes
Clonality Analysis by T-Cell Receptor PCR and High-Resolution Electrophoresis in the Diagnosis of Cu
Production of Tumor-Specific Antibodies in Tobacco
Primary Surgical Management of Ovarian Cancer
Nutrigenomics: Implications for Breast and Colon Cancer Prevention
Identification of Anaplastic Lymphoma Kinase Variant Translocations Using 5RACE
Tissue Microarrays
Oligonucleotide-Directed Microarray Gene Profiling of Pancreatic Adenocarcinoma
Treatment Strategies in Metastatic Renal Cell Carcinoma: Cytokines, Vaccines, and Gene Therapy
Studying MicroRNAs in Lymphoma
Measuring Drug-DNA Adducts in Individual Cells
Medical Management of Hepatocellular Carcinoma
The Epigenetics of Brain Tumors
Radioimmunohistochemistry: Quantitative Analysis of Cell Surface Receptors in Frozen Sections
Study Designs in Genetic Epidemiology
aking Sense of DNA Microarray Data
Determination of Biological Parameters on Fine-Needle Aspirates from Non-Small Cell Lung Cancer
LDI-PCR: Identification of Known and Unknown Gene Fusions of the Human MLL Gene
Pharmacogenomics
Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers
Single-Nucleotide Polymorphism (SNP) Analysis to Associate Cancer Risk
Pharmacokinetic and Pharmacodynamic Implications of P-Glycoprotein Modulation
CHK1 Kinase Activity Assay
Epidemiology of Brain Tumors
TUNEL Assay as a Measure of Chemotherapy-Induced Apoptosis
Molecular Analysis of Genetic Instability Caused by Chronic Inflammation
Inhibiting Proinflammatory NF-B Signaling Using Cell-Penetrating NEMO Binding Domain Peptides
Assessment of Gelatinases (MMP-2 and MMP-9 by Gelatin Zymography
Detection of t(2;5)(p23;q35)Translocation by Long-Range PCR of Genomic DNA
The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts
The Contribution of Niche-Derived Factors to the Regulation of Cancer Cells
Extraction of Nucleic Acid Templates
Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions
Inflammation and Tumor Progression: A Lesson from TNF--Dependent FAK Signaling in Cholangiocarcinoma
Economical Protocol for Combined Single-Strand Conformation Polymorphism and Heteroduplex Analysis o
Clonality Studies in Cancer Based on X Chromosome Inactivation Phenomenon
Gene Profiling Uncovers Retinoid Target Genes
Microarray-Based Transcriptome Profiling of Ovarian Cancer Cells
Newcastle Disease Virus: A Promising Vector for Viral Therapy, Immune Therapy, and Gene Therapy of C
Ribonomic and Short Hairpin RNA Gene Silencing Methods to Explore Functional Gene Programs Associate
Genetic Testing in Familial Melanoma: Epidemiologic/Genetic Assessment of Risks and Role of CDKN2A A
Overcoming Multidrug Resistance by RNA Interference
Functional Profiling Methods in Cancer
Design and Testing of Novel Oncolytic Vaccinia Strains
Hereditary Colon Cancer Genes
Xenotransplantation of Breast Cancers
Xenograft Models in Immunodeficient Animals: I. Nude Mice: Spontaneous and Experimental Metastasis M
Cytogenetic Studies in Hematologic Malignancies: An Overview
Flow Cytometric Analysis of MDM2-Mediated Growth Arrest
Breast Cancer Screening and Biomarkers
Comparative Genomic Hybridization and Fluorescence In Situ Hybridization in Chronic Lymphocytic Leuk
Orthotopic Model of Renal Cell Carcinoma
Assaying Cdc25 Phosphatase Activity
Application of Gene Microarrays in the Study of Prostate Cancer
Gene Expression Profiling of Leukemia Stem Cells
Orthotopic, Syngeneic Mouse Model to Study the Effects of EpithelialStromal Interaction
Pharmacogenetic Analysis of Clinically Relevant Genetic Polymorphisms
A Simple Method for Profiling miRNA Expression
Production and Characterization of Antipeptide Kallikrein 4 Antibodies: Use of Computer Modeling to
Phosphotyrosine Kinase Assays as a Prescreen for Inhibitors of EGFr
Metastatic Orthotopic Mouse Models of Lung Cancer
Elucidation of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes
An Epidemiologic and Clinicopathologic Overview of AIDS-Associated Pulmonary Kaposis Sarcoma
Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for
Anti-c-erb1B2 Ribozyme for Gene Therapy of Breast Cancer
Surrogate Markers of Angiogenesis and Metastasis
Identification of Lymphoma-Associated Antigens Using SEREX
Recombinant Adenoviruses for In Vivo Expression of Antibody Fragments
Quantitative Determination of p16 Gene Expression by RT-PCR
Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations
Tumor Suppressor Genes in Lung Cancer
Cell Separations by Flow Cytometry
Transforming Growth Factor Beta: A Plasma Tumor Marker
Strategies for the Use of Site-Specific Recombinases in Genome Engineering
Molecular Mechanisms of Drug Resistance in Single-Step and Multi-Step Drug-Selected Cancer Cells
In Vitro Model of Spontaneous Mouse OSE Transformation
Quantitative and Spatial Image Analysis of Tumor and Draining Lymph Nodes Using Immunohistochemistry
Engineering Hot Spots for Affinity Enhancement of Antibodies
Gene Expression Profile Analysis of Tumors
Use of the Polymerase Chain Reaction Technique to Detect the t(14;18) Translocation in Lymphoid Tiss
Nanoparticle Characterization for Cancer Nanotechnology and Other Biological Applications
Direct Kinase Assay Screening for Inhibitors of MAP Kinase
Using In Vivo Biopanning for the Development of Radiation-Guided Drug Delivery Systems
Analysis of Growth Properties and Cell Cycle Regulation Using Mouse Embryonic Fibroblast Cells
In Situ Hybridization in Cancer and Normal Tissue
DNA Immunization as a Means to Generate Antibodies to Proteins
Measurement of Estrogen Receptor Status by Immunocytochemistry in Paraffin Wax Sections
Quantitative PCR Detection of c-erbB-2 Gene Amplification
Identification and Analysis of Precursors to Invasive Pancreatic Cancer
RNA Purification and Quantification Methods
Use of siRNA to Study the Function of MDC1 in DNA Damage Responses
Complete Array of HOX Gene Expression by RQ-PCR
Production of Plasmid DNA as a Pharmaceutical
Functional Analysis of Tumor Suppressor Genes in Mice
Microarray-Based Comparative Genomic Hybridization (Array-CGH) as a Useful Tool for Identifying Gene
Immunoscope Analysis of T-Lymphocytes Infiltrating Melanocytic Tumors
The Chick Embryo Metastasis Model
The Regulation of Tumor Suppressor Genes by Oncogenes
Cancer Gene Profiling in Prostate Cancer
Detection and Analysis of DNA Methylation by Pyrosequencing
Non-RI Protocols for L-myc Allelotyping and Deletion Mapping of Chromosome 1p in Primary Lung Cancer
Cyclooxygenase Inhibitors Induce Colon Cancer Cell Apoptosis Via PPAR 14-3-3 Pathway
Apoptosis Assessment by the DNA Diffusion Assay
Epigenetic Targets in Cancer Epidemiology
Molecular and Genetic Aspects of Lung Cancer
Genetic Analysis of Drug Resistance by Fluorescence In Situ Hybridization
Detection of Apoptosis by the TUNEL Assay
Backtracking of Leukemic Clones to Birth
Integrating Molecular Biomarkers into Current Clinical Management in Melanoma
Proteomics and Cancer Epidemiology
Ovarian Cancer Stem Cells Enrichment
Epidemiological Investigation of Prostate Cancer
Immunohistochemical Evaluation of Biomarkers in Prostatic and Colorectal Neoplasia: Principles and G
Procedures for the Quantitative Protein Determination of Urokinase and Its Inhibitor, PAI-1, in Huma
Detection of the Transcripts and Proteins for the Transforming Growth Factor- Isoforms and Receptors
Use of Proteomics to Study Chemosensitivity
Analyzing Cell Cycle Checkpoints After Ionizing Radiation
Manipulating Genes and Gene Copy Number by Bacterial Artificial Chromosome Transfection
DNA Methylation and Histone Modifications in Breast Cancer
Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitati
Generation and Characterization of p53 Mutant Mice
G1 and S-Phase Checkpoints, Chromosome Instability, and Cancer
Flow Cytometric Measurement of Cell Proliferation
Incomplete DJH Rearrangements
The Impact of Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV): From Research to Clinical Practic
Identifying Obstacles to Viral Gene Therapy for Lung Cancer: Malignant Pleural Effusions as a Paradi
18 Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations
UroVysion Multiprobe FISH in Urinary Cytology
The Polymerase Chain Reaction
Analyzing the G2/M Checkpoint
Mutation of p53 Tumor Suppressor Gene in Hepatocellular Carcinoma
Identifying Genes Related to Chemosensitivity Using Support Vector Machine
Approaches to Proteomic Analysis of Human Tumors
Quantitative DNA Methylation Profiling on a High-Density Oligonucleotide Microarray
One-Dimensional Genome Scanning-Detection of Genomic Changes in Ovarian Carcinoma
s-RT-MELT: A Novel Technology for Mutation Screening
Microarray for Epigenetic Changes: Gene Expression Arrays
Chromosome Staining and Banding Techniques
In Situ Hybridization to Localize mRNAs
Utilization of SSCprofiler to Predict a New miRNA Gene
Detecting Mechanisms of Acquired BRAF Inhibitor Resistance in Melanoma
Cancer Gene Profiling in Pancreatic Cancer
Morphological Assessment of Apoptosis in Human Lung Cells
Mutation Detection inTGF- Receptors
Generation and Characterization of Bispecific Tandem Diabodies for Tumor Therapy
Pathology of Kidney Tumors
Developing Anti-HER2/neu Single-Chain Fv Fragments from Phage Display Libraries
Cytogenetic Analysis and Reporting
Array-Based Comparative Genomic Hybridization as a Tool for Analyzing the Leukemia Genome
Methylation Analysis of CpG Islands
Chromatin Immunoprecipitation Assays: Molecular Analysis of Chromatin Modification and Gene Regulati
Analysis of Transplanted Bone Marrow-Derived Cells in Chronic Pancreatitis
Isolation of a Purified Epithelial Cell Population from Human Colon
Inflammatory Response of Tumor-Activated Hepatic Sinusoidal Endothelium as a Target for the Screenin
Analyzing Markers of Apoptosis In Vitro
Measuring Gene Expression from Cell Cultures by Quantitative Reverse-Transcriptase Polymerase Chain
Quantitative Angiogenesis in Breast Cancer
FISH Techniques
Genetic Suppressor Elements in the Characterization and Identification of Tumor Suppressor Genes
Direction of the Recognition Specificity of Cytotoxic T Cells Toward Tumor Cells by Transduced, Chim
Transactivation and Transrepression Studies with p53
Energy Intake, Physical Activity, Energy Balance, and Cancer: Epidemiologic Evidence
Lymphoid Disorders Other than Common Acute Lymphoblastic Leukemia: Background
MicroRNA Profiling in Ovarian Cancer
PTEN and Cancer
Isolation and Culture of Leukemia Cell Lines
TP53, hChk2, and the Li-Fraumeni Syndrome
Caspase Activity Assays
Selection of Cytochrome P450 Genes for Use in Prodrug Activation-Based Cancer Gene Therapy
Electrophoretic Mobility Shift Analysis of the DNA Binding of Tumor Suppressor Gene Products
Application of SNP Genotype Arrays to Determine Somatic Changes in Cancer
Detection of K-ras Mutations by a Microelectronic DNA Chip
Integrating Multiple Clinical Tests to Increase Predictive Power
Generation and Application of Phospho-specific Antibodies for p53 and pRB
Tumor Vascular Therapy With TNF: Critical Review on Animal Models
Quantitation of CD39 Gene Expression in Pancreatic Tissue by Real-Time Polymerase Chain Reaction
Authentication of Cancer Cell Lines by DNA Fingerprinting
Tumor-Infiltrating Lymphocytes and Their Significance in Melanoma Prognosis
Aerosol Gene Therapy for Metastatic Lung Cancer Using PEI-p53 Complexes
Molecular Analysis of the von Hippel-Lindau Disease Gene
Recent Advances and Dilemmas in the Radiotherapeutic Management of Locally Advanced Non-Small Cell L
Generation of Recombinant Multimeric Antibody Fragments for Tumor Diagnosis and Therapy
Detecting DNA Methylation Using the Methylated CpG Island Amplification and Microarray Technique
Targeted Chemotherapy in Drug-Resistant Tumors, Noninvasive Imaging of P-Glycoprotein-Mediated Funct
Production and Characterization of Recombinant Human and Murine TNF
The Use of Phage Display Peptide Libraries for Basic and Translational Research
Basic Immunocytochemistry for Light Microscopy
Identification of DNA-Binding of Tumor Suppressor Genes by Chromatin Immunoprecipitation
Genetic Polymorphisms in the Transforming Growth Factor-β Signaling Pathways and Breast Cancer Risk
Quantitative Immunohistochemistry of Estrogen and Progesterone Receptor Positivity in Ovarian Tumors
Detection of Circulating Tumor Cells by Photoacoustic Flowmetry
Development of Bacterial Vectors for Tumor-Targeted Gene Therapy
Analysis of EMT by Flow Cytometry and Immunohistochemistry
Prediction of the Biological Effect of Polymorphisms Within MicroRNA Binding Sites
Comparative Genomic Hybridization
Breast Cancer Proteomics Using Two-Dimensional Electrophoresis: Studying the Breast Cancer Proteome
PCR Microsatellite Analysis of LOH in Ovarian Tumors
The EGFR Pathway as an Example for Genotype: Phenotype Correlation in Tumor Genes
Transfection Assays for Transformation of Model Colonic Cell Lines
Solving Problems in Multiplex FISH
Marker Utility for Combination Therapy
Compartmentalized Protein Kinase C Activation in Ovarian Carcinoma Cells
Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia
Molecular Diagnosis of Acute Myeloid Leukemia with Maturation, FAB-Type M2
Neurosphere Culture and Human Organotypic Model to Evaluate Brain Tumor Stem Cells
Matrix Metalloproteinases and Their Inhibitors
Laser Microdissection Sample Preparation for RNA Analyses
Functional Analysis of the Antimitogenic Activity of Tumor Suppressors
NanoparticleAptamer Conjugates for Cancer Cell Targeting and Detection
Gene Expression Profile Analysis of Lymphomas
Quantitative, Fluorescence-Based In-Situ Assessment of Protein Expression
Detection of TNF and TNF Receptor mRNA in Cells and Tissues
Manual Microdissection
Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD): In Vitro Microassays, Analysis of
Simultaneous Flow Cytometric Detection of DNA and Cellular Protein Molecules
Zebrafish as a Model for Pancreatic Cancer Research
Development of a Scoring System to Screen for BRCA1/2 Mutations
Primary Mouse Endothelial Cell Culture for Assays of Angiogenesis
Methods for Chemoprotection and Chemosensitization: MDR-1 For Chemoprotection Using Retroviruses to
Fluorescence In Situ Hybridization and Comparative Genomic Hybridization
Ethics of Cancer Gene Transfer Clinical Research
Lung-Specific Expression of Mutant p53 as Mouse Model for Lung Cancer
Metabolomics of Cancer
Classification of AML by DNA-Oligonucleotide Microarrays
Determining Mutational Status of Immunoglobulin V Genes in Chronic Lymphocytic Leukemia: A Useful Pr
Positional Approaches to Cancer Genetics
Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity
Tissue Resources for Clinical Use and Marker Studies in Melanoma
Analysis of Cancer Stem Cell Metastasis in Xenograft Animal Models
Clinical Implications of Epigenetic Alterations in Human Thoracic Malignancies: Epigenetic Alteratio
Generation of Genetically Modified Embryonic Stem Cells for the Development of Knockout Mouse Animal
Genome-Wide Methylation Analysis
High-Throughput Mutation Screening Using a Single Amplification Condition
Methods in Cancer Epigenetics and Epidemiology
Generation of Immortal Human Prostate Cell Lines for the Study of Prostate Cancer
In Vivo Particle-Mediated Gene Transfer for Cancer Therapy
In Vitro and In Vivo Apoptosis Detection Using Membrane Permeant Fluorescent-Labeled Inhibitors of C
Transposable Elements as Plasmid-Based Vectors for Long-Term Gene Transfer into Tumors
Energy Balance, Physical Activity, and Cancer Risk
Genetically Modified Clostridium for Gene Therapy of Tumors
Homogeneous, Bioluminescent Proteasome Assays
Adhesion Signaling Through Integrins
RT-PCR Analysis of Breakpoints Involving the MLL Gene Located at 11q23 in Acute Leukemia
Factors Affecting Immunohistochemical Evaluation of Biomarker Expression in Neoplasia
Storage of Cell Lines
Assessment of Local Proteolytic Milieu as a Factor in Tumor Invasiveness and Metastasis Formation: I
Bisulfite Methylation Analysis of Tumor Suppressor Genes in Prostate Cancer from Fresh and Archival
Developing Classifiers for the Detection of Cancer Using Multi-Analytes
Assaying the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae
Detection of Rearrangements in the bcl-2 Gene Using the Polymerase Chain Reaction
Meta-analysis of Cancer Gene-Profiling Data
Demonstration of a Germinal Center Immunophenotype in Lymphomas by Immunocytochemistry and Flow Cyto
Humanization of Monoclonal Antibodies by CDR Grafting
Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistry
Detection of Telomerase Activity by PCR-Based Assay
Epigenetics in Ovarian Cancer
Use of DNA Fingerprinting to Detect Genetic Rearrangements in Human Cancer
Analyzing the Function of Tumor Suppressor Genes Using a Drosophila Model
Clinical Implications of p53 Mutations in Lung Cancer
Detection of Apoptosis in Ovarian Cells In Vitro and In Vivo Using the Annexin V-Affinity Assay
Drug Delivery Approaches for Ovarian Cancer Therapy
Current Applications of Nanotechnology for Magnetic Resonance Imaging of Apoptosis
Genetic Manipulation of Yeast to Identify Genes Involved in Regulation of Chemosensitivity
Analysis of Cell Cycle Progression and Genomic Integrity in Early Lethal Knockouts
Multidrug Resistance in Cancer
Immunosuppressors as Multidrug Resistance Reversal Agents
Biochemical Analysis of the Native TRAIL Death-Inducing Signaling Complex
Targeting Damage-Associated Molecular Pattern Molecules (DAMPs) and DAMP Receptors in Melanoma
Pathological Assessment of Ovarian Cancer
Estrogen-Responsive Ovarian Cancer Xenografts
Reverse Phase Protein Microarrays for Monitoring Biological Responses
Multifactorial Etiology of Gastric Cancer
The Principles of MiRNA-Masking Antisense Oligonucleotides Technology
Flow Cytometric Detection of Activated Caspase-3
MUC1 Expression in Lung Cancer
Detection and Genotyping Analysis of Human Papillomavirus Isolates from Liquid-Based Cervical Cytolo
Colloidal Gold: A Novel Nanoparticle for Targeted Cancer Therapeutics
Bispecific Diabodies for Cancer Therapy
An Overview: From Discovery of Candidate Mutations to Disease Modeling and Transformation Mechanisms
Surgical Treatment of Lung Cancer: Past And Present
A Method for Conducting Highly Sensitive MicroRNA In Situ Hybridization and Immunohistochemical Anal
Cloning of Immunoglobulin Chromosomal Translocations by Long-Distance Inverse Polymerase Chain React
Clinical Application of Tissue and Serum Markers in Breast Cancer
Novel Approaches to Screen for Anticancer Drugs Using Saccharomyces cerevisiae
Prioritizing Candidate Genetic Modifiers of BRCA1 and BRCA2 Using a Combinatorial Analysis of Global
Abrogation of the RB-p16Tumor Suppressor Pathway in Human Lung Cancer
Challenges in Experimental Modeling of Ovarian Cancerogenesis
Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microd
Co-immunoprecipitation of Tumor Suppressor Protein-Interacting Proteins
Detection of Chromosomal Deletions by Microsatellite Analysis
Immunocytochemical Detection and Characterization of Individual Micrometastatic Tumor Cells
Microsatellite Instability Testing
Ultrasound Techniques for the Detection of Tumors and Metastases in Small Animals
Reconstruction of Geno-Phenotypic Evolutionary Sequences from Intracellular Patterns of Molecular Ab
Cytogenetics of Solid Tumours
Experimental Models and Methods in Antibody Targeting of Renal Cell Carcinoma
Cancer Biology
Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma
Methods for the Study of Protein-Protein Interactions in Cancer Cell Biology
Serial Analysis of Gene Expression (SAGE)
Immunophenotyping of Plasma Cells in Multiple Myeloma
Cytogenetic Methods in Chronic Lymphocytic Leukemia
Epigenetic Regulation of Multidrug Resistance 1 Gene Expression: Profiling CpG Methylation Status Us
Cytogenetic Analysis in Acute Myeloid Leukaemia
Ovarian Cancer Gene Therapy with BRCA1An Overview
Culture and Characterization of Human Ovarian Surface Epithelium
TNF in Experimental Diseases of the CNS
Cclooxygenase-2 (COX-2) Protein Expression by Western Blotting
Mesoporous Silicon Particles for Sustained Gene Silencing
Realizing the Potential of Ejaculate/Seminal Fluid in Detecting and Predicting Natural History
Renal Cell Carcinoma: Clinical Features and Management
Fluorescence In Situ Hybridization Methods and Troubleshooting Applied to Fixed Cell Suspensions
Analysis of the PML/RAR- Fusion Gene in Acute Promyelocytic Leukemia by Reverse-Transcription Polyme
Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay
Determination of Tumor Ferritin Concentration in Breast Cancer
Pooled ShRNA Screenings: Computational Analysis
Overview of Linkage Analysis: Application to Pancreatic Cancer
Immunohistochemistry of Pancreatic Neoplasia
Determination of Cyclin D1 Expression by Quantitative Real-Time, Reverse-Transcriptase Polymerase Ch
Xenografting and Harvesting Human Ductal Pancreatic Adenocarcinomas for DNA Analysis
Isolation of RNA From Tumor Samples: Single-Step Guanidinium Acid-Phenol Method
Use of Antisense Oligonucleotides for Therapy: Manipulation of Apoptosis Inhibitors for Destruction
Antigen-Pulsed Dendritic Cell Approach to Melanoma
Isolation of Endothelial Cells from Human Tumors
p53 Detection in Breast Cancer
Apoptosis Measurement by Annexin V Staining
Detection of Chromosome Abnormalities Using Cytoplasmic Immunoglobulin Staining and FISH in Myeloma
Screening for EpsteinBarr Virus in Hodgkin's Lymphoma
NPM-ALK Reverse Transcriptase-Polymerase Chain Reaction Analysis for Detection of the t(2;5) Translo
Main Principles and Outcomes of DNA Methylation Analysis
Energy Metabolism and Changes in Cellular Composition in Ovarian Cancer
Diagnostic and Prognostic Significance of the Methylation Status of Myf-3 in Lymphoproliferative Dis
Conventional Cytogenetics in Myeloma
Preparation and Characterization of Doxorubicin Liposomes
Cell Culture Contamination
Use of Gene Gun for Genetic Immunotherapy: In Vitro and In Vivo Methods
Ex Vivo Assays to Study Self-Renewal and Long-Term Expansion of Genetically Modified Primary Human A
The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following
In Vitro Differentiation of Embryonic Stem Cells as a Model of Early Hematopoietic Development
T-Cell Receptor Clonotype Mapping Using Denaturing Gradient Gel Electrophoresis: Analysis of Clonal
Patched, Hedgehog, and Skin Cancer
Preparation of Metaphase Chromosomes for Cytogenetic Analysis
Measurement of Reduced Glutathione Using High-Pressure Liquid Chromatography
The INK4a/ARF Locus and Human Cancer
c-myb Antisense Oligonucleotide Therapeutics for Hematologic Malignancies
DNA Methylation Analysis in Human Cancer
In Vitro Micronucleus Technique to Predict Chemosensitivity
Genes Involved in the Metastatic Cascade of Medullary Thyroid Tumours
Mutation Analysis of Cancer Using Automated Sequencing
Immunodiagnosis of Childhood Malignancies
Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics
Molecular Basis of Histone Deacetylase Inhibitors as New Drugs for the Treatment of Inflammatory Dis
Anti-MDR1 Ribozyme Gene Therapy
MicroRNA Profiling Using Fluorescence-Labeled Beads: Data Acquisition and Processing
Cytogenetic Characterization of Tumor Cell Lines
Overview of Tumor Cell Chemoresistance Mechanisms
Deep Transcriptome Profiling of Ovarian Cancer Cells Using Next-Generation Sequencing Approach
Analysis of Checkpoint Responses to Histone Deacetylase Inhibitors
Genetic Abnormalities in Non-Hodgkins Lymphoma as Revealed by Conventional and Molecular Cytogenetic
Measurement of Apoptotic Cells by Flow Cytometry (Tunnel Assay)
Sensitive Detection of Hypermethylated p16INK4a Alleles in Exfoliative Tissue Material
RNA Networks in Ovarian Cancer
红细胞脆性的测试
Anti-HMdU Autoantibodies in Human Sera as a Biomarker of Cancer Risk
Other Lymphoid Malignancies: Cytogenetic Techniques
Oncolytic Adenoviral Vectors
Isolation and Culture of Human Brain Tumor Cells
Image Analysis Using the Fluorochromasia Assay to Quantify Tumor Drug Sensitivity
Assessment of Cellular Proliferation by Calculation of Mitotic Index and by Immunohistochemistry
Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning
Angiogenesis Links Chronic Inflammation with Cancer
Measurement of P-glycoprotein Function
Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay
Flow Cytometric Measurement of Functional and Phenotypic P-Glycoprotein
Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors
Epigenetic Biomarkers in Cancer Epidemiology
Strategies for Improving the Anti-Neoplastic Activity of TNF by Tumor Targeting
Antisense Libraries to Isolate Tumor Suppressor Genes
Tumor Vaccination with Cytokine-Encapsulated Microspheres
Humanized Model to Study Leukemic Stem Cells
Survival Prediction Based on Inherited Gene Variation Analysis
Wilms Tumor Gene WT1 as a: Tumor Marker for Leukemic Blast Cells and Its Role in Leukemogenesis
Isolation and Culture of Squamous Cell Carcinoma Lines
Epidemiology of Human Papilloma Virus (HPV) in Cervical Mucosa
Hormones as Biomarkers: Practical Guide to Utilizing Luminex Technologies for Biomarker Research
Detection of the Replication Error Phenotype in Ovarian Cancer-PCR Analysis of Microsatellite Instab
Immunohistochemical Assays of Farnesyltransferase Inhibition in Patient Samples
AIDS-Associated Pulmonary Cancers
Laser-Capture Microdissection
Production and Characterization of Receptor-Specific TNF Muteins
Functional Analysis of the Spindle-Checkpoint Proteins Using an In Vitro Ubiquitination Assay
Quality Assurance of Predictive Markers in Breast Cancer
Development of Rituximab-Resistant B-NHL Clones: An In Vitro Model for Studying Tumor Resistance to
The Prevention and Genetics of Pancreatic Cancer: A Programmatic Approach
MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Transloca
Circumventing Tumor Resistance to Chemotherapy by Nanotechnology
Mechanisms of Multidrug Resistance in Cancer
Syngeneic Murine Metastasis Models: B16 Melanoma
Establishment of Primary Cultures from Ovarian Tumor Tissue and Ascites Fluid
Tumor Markers in Screening for Ovarian Cancer
Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of
An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors
Analyzing Checkpoint Controls in Human Skin
Role of Cytokines in Pathology of Melanoma and Use of Biologic Response Modifiers in Melanoma
Mapping of Tumor Suppressor Genes in Ovarian Cancers
Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction
Measurement of Telomerase Activity in Human Hematopoietic Cells and Neoplastic Disorders
Obesity Provides a Permissive Milieu in Inflammation-Associated Carcinogenesis: Analysis of Insulin
Multiplex Amplifiable Probe Hybridization (MAPH) Methodology as an Alternative to Comparative Genomi
Evaluation of Chemosensitivity of Micrometastases with Green Fluorescent Protein Gene-Tagged Tumor M
Development of a Cytokine-Modified Allogeneic Whole Cell Pancreatic Cancer Vaccine
Use of RNA Aptamers for the Modulation of Cancer Cell Signaling
Clinically Relevant Metastatic Breast Cancer Models to Study Chemosensitivity
Virus Production for Clinical Gene Therapy
Cell Cycle Checkpoint Control Mechanisms That Can Be Disrupted in Cancer
Expression Analysis of Homeobox Genes in Leukemia/Lymphoma Cell Lines
Antisense Oligonucleotide Inhibitors of MDM2 Oncogene Expression
Use of Nanoparticles for Targeted, Noninvasive Thermal Destruction of Malignant Cells
Digital Single-Nucleotide Polymorphism Analysis for Allelic Imbalance
Single-Chain Fv-Based Affinity Purification of the Cellular Stress Protein gp96 for Vaccine Developm
Isolation of Circulating MicroRNAs from Microvesicles Found in Human Plasma
Epigenetics of Solid Cancer Stem Cells
Enhanced Mismatch Mutation Analysis: Simultaneous Detection of Point Mutations and Large Scale Rearr
Rapid Identification of Chromosomes Using Primed In Situ Labeling (PRINS)
Generating Inhibitors of P-Glycoprotein: Where to, Now
Generation, Expression, and Monitoring of Recombinant Immune Receptors for Use in Cellular Immunothe
Immunological and Gene Therapy for Ovarian Cancer A Technical Overview
Identification of Murine and Human Acute Myeloid Leukemia Stem Cells
Preparation of Retroviral Vectors for Cell-Cycle-Targeted Gene Therapy of Lung Cancer
Sodium Dodecyl SulphatePolyacrylamide Denaturing Gel Electrophoresis and Western Blotting Techniques
Cancer Screenings, Diagnostic Technology Evolution, and Cancer Control
The Tissue Microenvironment as an Epigenetic Tumor Modifier
Adenovirus-Mediated Targeted Gene Therapy for Breast Cancer and for Purging Hematopoietic Stem-Cell
Do Viruses Cause Breast Cancer
Immunologic Monitoring of Cancer Vaccine Trials Using the ELISPOT Assay
Monoclonal Antibodies: Generation and Clinical Use
Adenovirus-Mediated Drug-Sensitivity Gene Therapy for Hepatocellular Carcinoma
Understanding Metastasis Through Integrin Expression
Circulating Tumor Cells as Prognostic Biomarkers in Cutaneous Melanoma Patients
Fluorescence In Situ Hybridization to Chromosomes
Alterations in the Expression of Insulin-Like Growth Factors and Their Binding Proteins in Lung Canc
Identification of Pancreatic Cancer-Specific Cell-Surface Markers for Development of Targeting Ligan
Application of Phage Display to Discovery of Tumor-Specific Homing Peptides: Developing Strategies f
Identification of Malignant Plasma Cells by mRNA In Situ Hybridization
Pharmacogenetics of ATP-Binding Cassette Transporters and Clinical Implications
Mutational Analysis of Oncogenes and Tumor Suppressor Genes in Human Cancer Using Denaturing Gradien
Promoter Methylation in Head and Neck Tumorigenesis
Microarray Approaches for Analysis of Tumor Suppressor Gene Function
Impact of Breast Cancer Resistance Protein on Cancer Treatment Outcomes
Characterization and Authentication of Cancer Cell Lines: An Overview
Detection of K-ras Point Mutations in Sputum from Patients with Adenocarcinoma of the Lung by Point-
Gene Expression Profiling in Cervical Cancer: Identification of Novel Markers for Disease Diagnosis
Assessment of Predictive Values of Tumor Markers
Liposomal Doxorubicin and nab-Paclitaxel: Nanoparticle Cancer Chemotherapy in Current Clinical Use
Interaction of p53 with the Adenovirus E1B-55 kDa Protein
Isolation of DNA From Tumor Samples
The Rat Aortic Ring Assay for In Vitro Study of Angiogenesis
Basic Principles of Cancer Cell Culture
Detection of K-ras and p53 Mutations by Mutant-Enriched PCR-RFLP
In Vivo and In Vitro Properties of Ovarian Cancer Cells
Methods for Analyzing Checkpoint Responses in Caenorhabditis elegans
Cancer Epidemiology in the United States: Racial, Social, and Economic Factors
More Than One Way to Die: Methods to Determine TNF-Induced Apoptosis and Necrosis
In Situ Hybridization Detection of TGF- mRNA
Anti-K-ras1 Ribozyme Adenoviral Vector for Gene Therapy of Non-Small Cell Lung Cancer
Cell Adhesion Assays
Radial Monolayer Cell Migration Assay
Characteristic Genetic Alterations in Lung Cancer
Detection and Analysis of Lung Cancer Cells from Body Fluids Using a Rare Event Imaging System
Integrative Prediction of Gene Function and Platinum-Free Survival from Genomic and Epigenetic Featu
c-erbB-2 Immunohistochemistry in Paraffin Tumors
cDNA Microarray Analysis of Inflammatory Breast Cancer Signatures
A Prognostic Test to Predict the Risk of Metastasis in Uveal Melanoma Based on a 15-Gene Expression
Polymerase Chain Reaction for Detection of the t(14;18) Translocation in Lymphomas
Peptide YY Mediates Inhibition of Tumor Growth and Inflammation
Melanoma Susceptibility Genes and Risk Assessment
Modulation of Apoptosis to Reverse Chemoresistance
Detection of Small Cell Lung Cancer by RT-PCR for Neuropeptides, Neuropeptide Receptors, or a Splice
Pituitary Adenoma Stem Cells
A Hepatitis C Virus Xenograft Mouse Efficacy Model
Acute Lymphoblastic Leukaemia
Spectral Karyotyping in Cancer Cytogenetics
Instrumentation, Accuracy, and Quality Control Issues in Development of Quantitative Fluorescence-Im
Fluorescence In Situ Hybridization (FISH) as a Tool for the Detection of Significant Chromosomal Abn
Feeder Layers: Co-culture with Nonneoplastic Cells
Evolution of Cytogenetic Methods in the Study of Cancer
Oncolytic Viral Therapy Using Reovirus
The Mammalian Two-Hybrid Assay for Detection of Coactivator-Nuclear Receptor Interactions
Manual Microdissection Combined with Antisense RNALongSAGE for the Analysis of Limited Cell Numbers
Methods to Study p53-Repressed Promoters
Molecular-Targeted Therapy for Cancer and Nanotechnology
Adenovirus-Mediated Wild-Type p53 Gene Transfer into Head and Neck Cancers
Assaying for BRAF V600E in Tissue and Blood in Melanoma
Assays for Neoplastic Cell Enrichment in Bone Marrow Samples
Development of Drug-Resistant Models
Modeling Pancreatic Cancer in Animals to Address Specific Hypotheses
Introduction
Retroviral Transfer of the Herpes Simplex Virus-Thymidine Kinase (HSV-tK) Gene for the Treatment of
Gene Therapy for Antitumor Vaccination
Detection of Prostate-Cancer Cells in Blood and Bone Marrow by RT-PCR
Photoacoustic Tomography for Imaging Nanoparticles
Activation of Caspase Protease During Apoptosis in Ovarian Cancer Cells
Flow Cytometric Methods for Detection and Quantification of Apoptosis
Isolation and Culture of Ovarian Cancer Cells and Cell Lines
Phase I Clinical Trial of Locoregional Administration of the Oncolytic Adenovirus ONYX-015 in Combin
Membrane Invasion Culture System
Detailed Analysis of Promoter-Associated RNA
Comparative Genomic Hybridization for the Analysis of Unbalanced Chromosomal Abnormalities in Ovaria
Quality Assurance and Good Laboratory Practice
E1A-Mediated Gene Therapy
Microtiter Plate Technique for the Measurement of Glutathione in Fresh and Cryopreserved Lymphoblast
Assessing Growth and Response to Therapy in Murine Tumor Models
Detection of Cancer-Related Proteins in Fresh-Frozen Ovarian Cancer Samples Using Laser Capture Micr
Differential Staining Cytotoxicity Assay: A Review
Purification of TNF Binding Proteins
Detection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage Assay
Molecular Genetics of Ovarian Cancer: A Technical Overview
Cultures of Surgical Material from Lung Cancers: A Kinetic Approach
Hybrid Cell Vaccination in Patients With Metastatic Melanoma
Effects of Fixation on Tissues
Bispecific Antibody MDX-210 for Treatment of Advanced Ovarian and Breast Cancer
Establishment and Culture of LeukemiaLymphoma Cell Lines
Gene Therapy for Lung Cancer: An Introduction
Cytogenetic and Genetic Studies in Solid Tumors: Background
Generation of Recombinant Immunotoxins for Specific Targeting of Tumor-Related Peptides Presented by
MicroRNA Northern Blotting, Precursor Cloning, and Ago2-Improved RNA Interference
Determination of Telomerase Activity and Telomere Length
Helicobacter -Based Mouse Models of Digestive System Carcinogenesis
Mutational Screening of hMLH1 and hMSH2 that Confer Inherited Colorectal Cancer Susceptibility Using
Mammographic Density: A Heritable Risk Factor for Breast Cancer
Xenograft Models in Immunodeficient Animals: II. The Use of SCID Mice in Metastasis Research: Breast
Hereditary Breast and Ovarian Cancer Genes
Generation and Analysis of Brca1 Conditional Knockout Mice
Autoantibodies Against Cancer Antigens
Proteomics in the Analysis of Prostate Cancer
Gene Amplification of c-erbB-2 Detected by FISH
Emerging Clinical Issues in Melanoma in the Molecularly Targeted Era
Primary Explant Cultures of Adult and Embryonic Pancreas
Regulation of NF-B by Oncoproteins and Tumor Suppressor Proteins
Localization of Cyclooxygenase-2 Protein Expression in Lung Cancer Specimens by Immunohistochemical
MTT Growth Assays in Ovarian Cancer
Apoptosis Detection by DNA Analysis
Xenopus Cell-Free Extracts to Study the DNA Damage Response
Anchorage-Independent Growth of Prostate Cancer Stem Cells
Mutation Detection in Colorectal Cancers: Direct Sequencing of DNA Mismatch Repair Genes
Gene Expression Microarrays
Detection of Apoptosis in Renal Cell Carcinoma
I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer
Reciprocal Subtraction Differential RNA Display (RSDD): An Efficient Technology for Cloning Differen
Invasion and Motility Assays
Pathological Evaluation of Axillary Sentinel Lymph Nodes in Breast Cancer
Intratumoral Injection of Naked DNA
Measurement of IGF-1 Receptor Content in Tissues and Cell Lines by Radioimmunoassay (RIA) and ELISA
VHL and Kidney Cancer
Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assa
Integrating Multiple Types of Data to Identify MicroRNAGene Co-modules
Generation of Antibody Molecules Through Antibody Engineering
Interphase Cytogenetics in Frozen Ovarian Tumor Tissue
Radioreceptor Measurement of ER/PR Giovanni Scambia, Gabriella Ferrandina, G. DAgostino,
Linking the Kaposi's Sarcoma-Associated Herpesvirus (KSHV/HHV-8) to Human Malignancies
Real-Time Cytotoxicity Assays
Single-Strand Conformation Polymorphism Mutation Analysis of the p53 Gene
MRD Detection in Acute Lymphoblastic Leukemia Patients Using Ig/TCR Gene Rearrangements as Targets f
Flow Cytometric DNA Analysis of Human Cancers and Cell Lines
Assessment of TNFα-Induced Endothelial Damage Through the Loss of Its Barrier Function
Transformation of the Human Ovarian Surface Epithelium with Genetically Defined Elements
Molecular Epidemiology of DNA Repair Genes in Bladder Cancer
Reverse-Phase Protein Microarrays for Theranostics and Patient Tailored Therapy
Analysis of HSV Oncolytic Virotherapy in Organotypic Cultures
Analysis of Telomerase Activity and Telomere Function in Cancer
Murine Animal Model
Purification of Recombinant p53 from Sf9 Insect Cells
Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinog
Functional Analysis of Human BRCA2 Variants Using a Mouse Embryonic Stem Cell-Based Assay
Identification of p53 Target Genes by Fluorescent Differential Display
Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization
The Use of Early Sea Urchin Embryos in Anticancer Drug Testing
Construction and Characterization of an Oncolytic HSV Vector Containing a Fusogenic Glycoprotein and
Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation
Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cell Culture
Assessment of Angiogenic Factors The Chick Chorioallantoic Membrane Assay
Computational Identification of miRNAs Involved in Cancer
Human Prostate Cancer Cell Lines
Elimination of Mycoplasmas from Infected Cell Lines Using Antibiotics
Molecular Karyotyping for Detection of Prognostic Markers in Fine Needle Aspiration Biopsy Samples o
PCR of Gene Rearrangements for the Detection of Minimal Residual Disease in Childhood ALL
A Novel and Simple Method to Assay the Activity of Individual Protein Kinases in a Crude Tissue Extr
Assessing the Interaction of Particulate Delivery Systems with Lung Surfactant
Analysis of Loss of Heterozygosity in Circulating DNA
Genetic Alterations in Esophageal Cancer
Identification of Altered Gene Expression Associated with Pigmentary Lesions by Differential Display
Cell Culture Contamination: An Overview
The Molecular Pathogenesis of Ovarian Cancer
Multiplex PCR (MPCR) Screening Can Detect Small Intragenic p53 Deletion and Insertion Mutations
Statistical Considerations in the Analysis of Tumor Markers
Relationship of EGFR Signal-Transduction Modulation by Tyrosine Kinase Inhibitors to Chemosensitivit
Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex
Imaging Specific Cell Surface Protease Activity in Living Cells Using Reengineered Bacterial Cytotox
Molecular Epidemiology of Human Cancer Risk: Gene-Environment Interactions and p53 Mutation Spectrum
Purification and Analysis of Checkpoint Protein Complexes From Saccharomyces cerevisiae
Magnetic Resonance Imaging of Metastases in Xenograft Mouse Models of Cancer
Targeting of Nanoparticles: Folate Receptor
Laser-Assisted Microdissection and Isolation of DNA and RNA
Induced Nitric Oxide Synthase as a Major Player in the Oncogenic Transformation of Inflamed Tissue
Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-Induced Apoptosis
Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies
Immunomagnetic Cell Separation
Molecular Analysis of Cancer: An Overview
Detecting Mismatch Repair Defects in Myeloma
Gene Expression Profiling to Characterize Anticancer Drug Sensitivity
Immunohistochemistry of p53, Bcl-2, and Ki-67 as Predictors of Chemosensitivity
DNA-Dependent Protein Kinase in Apoptosis
PARP Cleavage as a Means of Assessing Apoptosis
Dietary and Lifestyle Factors of DNA Methylation
Biomarkers of Cell Proliferation in Carcinomas: Detection of Angiogenesis and Infiltrated Leukocytes
Traditional and Established Indicators of Prognosis and Treatment Success
Calibration of Microarray Gene-Expression Data
Gene Targeting to Hepatomas (AFP)
Measurement of Protein Expression of p53, p21WAF1, and Rb in Patients with Surgically Treated Hepato
Assays for Proteasome Inhibition
Direct Analysis for Familial Adenomatous Polyposis Mutations
Gene Therapy of Glioblastoma Multiforme with a Bicistronic Retroviral Vector Expressing Human IL-2 a
Rescue and Isolation of Rb-deficient Prostate Epithelium by Tissue Recombination
Double- and Competitive-Differential PCR for Gene Dosage Quantitation
Real Time In Vivo Quantitation of Tumor Angiogenesis
稳转用什么型号的脂质体
EB病毒诱导淋巴细胞
胰腺上皮内瘤变和胰腺癌组织中TGF-f3/Smad信号通路相关蛋白表达
肝刺激因子对人肝癌细胞BEL-7402 p21ras表达的影响
一种实用的遗传诱导预定命运法：可视化引导细胞的标记与追踪
实时细胞分析系统有望为中药检测提供可量化依据
膜片钳记录和分析技术
流式细胞术应用 | 囊泡检测步骤详解
利用生物反馈技术测定心理生理压力
利用可光合作用的海藻细胞产生电流
Di-8-ANEPPS测定感应膜电压
免疫研究新方法：IGF信号通路抗体芯片筛选出肝癌靶生物标志物
细胞信号传导通路最优模型
细胞信号传导通路最优新模型
细胞通信
在体多通道记录技术的应用及实验流程
中性粒细胞细胞外捕获——如何产生和观察
Wnt/β-catenin信号通路在实验性大鼠肝癌形成过程中的作用
CHO细胞的稳定表达
实时监测单个细胞间相互作用
突破传统的一分钟转染--QuickShuttle系列转染试剂
我的信号通路研究
白血病干细胞最新研究成果
cAMP、cGMP 信号转导通路
利用全内反射荧光显微镜对视网膜双级细胞进行胞吐作用的成像
p53抑癌基因的基因观
实验秘笈 | 细胞冻存与复苏
基因打靶细胞系的制备及应用
浅谈一般的细胞传代方法
细胞分离密度梯度离心原理
正常人外周血白细胞的分离
原代肺泡上皮细胞的体外分离培养
骨髓细胞染色体标本的制备及观察
人调节性T细胞分析
HeLa细胞裂解+细胞核提取+分离纯化线粒体
PriCells: 正常人角膜上皮细胞和干细胞的分离
小鼠单个核细胞的分离
从血液和组织中提取RNA的方法及其区别
细胞培养液中蛋白的浓缩收集方法
细胞破碎的方法
如何收集细胞（用于western blot等）
加多了细胞裂解液是否有补救措施
肺泡上皮细胞的分离
测定细胞乳酸脱氢酶释放率的若干问题
PriCells: 正常人原代角膜间质细胞的分离
Sysmex R-3500全自动血细胞分析仪使用
软骨细胞培养
细胞裂解
关于DNA LADDR提取柱子的选择
Tris饱和苯酚（Tris saturated phenol）也称Tris平衡酚（Tris balanced phenol），是添加了抗氧化剂8-羟基喹啉，tris pH8.0充分平衡的苯酚，呈黄色。
Tris饱和酚和重蒸酚的区别和配制方法
PriCells: 从人关节软骨中分离软骨祖细胞
RIPA裂解液配方
细胞/组织裂解液
如何验证提取出的褐色物质是线粒体
细胞破碎
不用Trizol即可裂解细胞提取RNA的方法
人CD40活化B细胞的分离
分离纯化酵母细胞线粒体
提取细胞RNA的方法
细胞凋亡提取DNA ladder的操作规程
《Handbook of Industrial Cell Culture》
血细胞分析的参考值
固定蠕虫的油红染色
用FDA染色测定花粉的育性和活力
转化生长因子-β（TGF-β）
间接免疫荧光法测抗体
激素、神经肽及神经递质对免疫功能的影响
HLA的临床应用
HLA与器官、骨髓移植
粘附分子的功能
CD3γ、δ和 ε链
磷脂酶C
MHC对免疫应答中免疫细胞相互作用的限制（约束）作用
其它细胞因子
MHC中的单体型
免疫功能在神经及内分泌组织中的体现
免疫球蛋白基因的结构和多样性
同种限制因子
T淋巴细胞亚群
蛋白酪氨酸激酶
细胞因子对神经内分泌系统的影响
人HLA基因图及其遗传特征
Ⅱ型补体受体（CD21）
免疫网络的其它模型
C2分子
PKC的转位与激活
H因子
肿瘤坏死因子
C8分子
B因子
NK细胞的功能
T淋巴细胞及其亚群
MHC抗原的结构及检测
免疫荧光技术之染色方法
CD、粘附分子与Ig超家族的关系
应激对免疫系统的影响
可溶性细胞因子受体
信号转导中第二信使含量的测定
细胞因子受体中的共用链
T细胞库的多样性
十、D因子
C3分子
三种细胞免疫荧光染色操作步骤
补体的遗传学特征
CD3ζ和η链
Ⅴ型补体受体
selectin家族
Ⅳ型补体受体
细胞核转录因子的分离和鉴定
膜辅蛋白（CD46）
条件反射对免疫功能的影响
补体的结构及遗传学特征
促衰变因子（CD55）
C4分子
NK细胞的表型
CD45在信号转导中的调节作用
SH-2结构域的调节作用
P因子
MHC 四聚体应用：TCR 样抗体研发
蛋白酪氨酸磷酸酯酶
核酸标记技术检测法
C5分子
细胞因子的结构和生物学特征
神经系统定位损毁对免疫功能的影响
神经和内分泌（或神经内分泌）系统对免疫系统的调控
免疫球蛋白超家族--　免疫球蛋白超家族的组成和特点
B细胞的膜表面分子
TCR基因的重排
免疫荧光技术之荧光显微镜检查方法
免疫荧光实验的几个问题
抗独特型的分类
BCR相关联的分子
粘附分子与细胞内信号
Ⅲ型补体受体（CD11b/CD18）
白细胞分化抗原--　白细胞分化抗原的分类
免疫网络学说
T细胞受体基因
T细胞基因的转录激活及其表达
抗独特型抗体在医学中的应用
过敏毒素灭活剂
I型补体受体（CD35）
干扰素（IFN）
神经、免疫及内分泌系统间的关系
C6和C7
MHC对免疫应答的遗传控制
胸腺肽对神经内分泌功能的影响
S蛋白
肿瘤浸润淋巴细胞
粘附分子--　粘附分子的种类和结构
C4结合蛋白
神经免疫内分泌调节环路
SP40/40
细胞因子基因
MHC参与免疫细胞识别抗原
二、 Ig超家族的特点
神经、免疫、内分泌系统间的关系
淋巴细胞群及其亚群--　B淋巴细胞的分化和表面标志
三、细胞因子受体基因
蛋白激酶C
补体受体的结构及功能
I因子
补体型
MHC的生物学功能
免疫球蛋白基因表达的调节
LAK细胞
神经免疫内分泌学引论--　神经免疫内分泌学的发展简史
主要组织相容性复合体--　MHC基因图及其遗传特征
HLA抗原的检测
免疫球蛋白的多样性
粘附分子的表达的调节
CD59
免疫网络学说及其在医海陆空中的应用--　独特型和抗独特型
趋化因子
三、独特型的分布
p59fyn
补体调节分子的结构及功能
细胞因子及其受体--　细胞因子的概念和作用特点
粘附分子参与免疫细胞的识别作用
抗独特型抗体及其在医学研究中的应用
免疫细胞中的凋谢
独特的分类
淋巴细胞信号转导研究中常用方法
C9分子
补体的分子生物学
经B淋巴细胞抗原受体介导的信号转导分子基础
HLA其他临床意义
MHC扩展单体型
免疫系统对神经内分泌系统的调控
G蛋白和磷脂酶C
胞浆或胞膜细胞因子（或受体）的检测
PCR介导的信号转导途径
自然杀伤细胞
支原体污染以及检测
细胞自噬的研究方法介绍
杂交瘤细胞株稳定性的检查
支原体之检测：直接培养法
如何用台盼兰计数活细胞
Ammonium Sulfate Binding Assay for Cells
荧光标记法检测细胞凋亡
肿瘤原代细胞鉴定技术
原代细胞鉴定技术
细胞调亡的几种检测方法
细胞消化的个人总结
MTT试验——细节决定成败（三）
细胞中的微丝染色及微丝与细胞形态的实验观察
CELLULAR ELECTRON MICROSCOPY
显微摄影术
人源细胞表达高活性天然细胞因子：细胞因子表达技术新趋势
“黏着斑”的分子架构被确定
你的细胞受到支原体污染了么？
斑马鱼神经元、神经嵴胚胎干细胞细胞运动和肌动蛋白的动态成像
HeLa细胞的形态问题
细胞形态不好的原因分析
L929培养过程中的异常形态问题
A549细胞到底是如何形态
用有机溶剂固定细胞爬片、甩片或切片
肿瘤细胞培养
细胞计数方法
细胞凝集反应与细胞膜的渗透性
扫描电子显微镜
PI染色法（活细胞染色）检测细胞凋亡
血小板的活化、荧光染色与流式细胞仪分析
人脐带平滑肌细胞培养
In situ hybridization of mammalian cells (RNA and Oligonucleotide probes)
电子显微镜检测法检测细胞凋亡
凋亡指数和细胞活力的定量测定（荧光染色法）
原位同时观察病毒特异性CD8+ T细胞和病毒感染细胞
51Cr释放试验测定NK细胞活性
各种荧光检测方法简介
组织和细胞的固定方法
DNA ladder法检测细胞凋亡
DAPI染色法检测细胞凋亡
末端转移酶标记技术（TUNEL法）检测细胞凋亡
癌症常见症状大盘点
Caspase 3 活性测定（FIENA法）检测细胞凋亡
细胞免疫荧光步骤
激光共聚焦的前期步骤
白细胞计数法（试管法）
单细胞凝胶电泳步骤
Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets
红细胞形态学诊断技巧（一）
如何让支原体滚远点
Optical Imaging Techniques in Cell Biology
Paraformaldehyde Fixation of Cells（多聚甲醛固定细胞）
靶细胞DNA片段的定量分析与杀伤细胞介导的细胞溶解试验
Trypan blue viability test
组织化学凝集素的标记物
组织常用固定剂和固定方法
采用 CytoFLEX 对 PBMC 的 T 细胞受体进行表征分析
荧光组织化学与组织细胞荧光分类
乙酰胆碱酯酶和胆碱酯酶组织化学染色法
乙醇苏丹黑B脂类染色法
一氧化氮合酶组织化学染色法
Hoechst染色
溴化乙啶染色
邻苯二醛显示组胺荧光法
Falck-Hillarp甲醛诱发荧光法
三磷酸胶瞽酶钙―钴法
酸性磷酸酶金属盐―铅法
酶组织化学的基本原理
细胞色素氧化酶二氨基联苯胺显示法（DAB法）
乙醛酸诱发生物单胺荧光法
酸性磷酸酶重氮盐后偶联法
【视频】手把手教你做碘化丙啶染色
生物素标记凝集素的组织化学染色程序
乳酸脱氢酶硝基蓝四唑盐法
亲和素―生物素―过氧化物酶复合物法
葡萄糖-6-磷酸酶组织化学染色法
培养细胞常用固定剂和固定方法
灭活内源性酶及封闭内源性生物素
链霉菌抗生物素蛋白―过氧化物酶法
碱性磷酸酶偶氮―偶联法
碱性磷酸酶钙―钴法
过氧化物酶TMB法
过氧化物酶DAB法
非特异性酯酶组织化学染色法
碘化丙啶染色
免疫组织化学抗原修复
吖啶橙原值区分组织细胞DNA和RNA的荧光组织化学染色法
吖啶橙区分活细胞DNA和RNA的荧光组织化学染色法
细胞染色方法总结
Hoechst 33258染色
FITC标记凝集素的组织化学染色程序
Feulgen反应原理及Sohiff液的配制
细胞的碘化丙啶染色用于FACS分析
DAPI染色
脂类组织化学与酶组织化学
如何有效提高转染效率
酶组织化学对组织处理的基本要求
十五种细胞染色技术
琥珀酸脱氢酶硝基蓝四唑盐法
肝脏的结构与肝炎、肝硬化和肝细胞癌的观察
常规切片的制作过程
胃的组织结构和胃癌的观察
肺组织结构与大小叶性肺炎的观察
显微放射自显影
病毒转化细胞技术
影响转染效率的主要因素
关血清使用中的常见问题解答
肿瘤细胞细胞系的培养
细胞凋亡相关基因与人类肿瘤
细胞分化与肿瘤
TIL （肿瘤浸润淋巴细胞）细胞的培养
质膜及其表面结构
植物细胞大规模培养技术
Percoll密度梯度离心法分离嗜碱性粒细胞
表皮细胞培养
昆虫细胞低MOI杆病毒的感染
MG63细胞
细胞凋亡检测-形态学特征的检测方法
Use of the Hemacytometer for the Determination of Cell Numbers
HeLa细胞株
甲醇酵母表达的试验步骤
3种方法测定血液中微量白细胞计数效果的比较研究
细胞核
细胞伤口愈合实验
原代细胞的脂类染色方法苏丹红Ⅲ染色法
染色体G-带的分带技术
传代培养及其操作步骤
什么是原代细胞、细胞株、细胞系？
原代肝细胞培养、肝非实质细胞、肝脏干细胞
原代培养及其操作步骤
细胞系（cell line）或细胞株（cell strain）的建立、鉴定、管理
其他细胞培养用液
实验性细胞移植：临床前及临床研究
细胞培养污染对策及预防方法
Vicia Faba and Chemical Damage
提醒有关购买国外细胞株应该注意的事项
染色技术
比血球计数板更方便准确的细胞计数方法推荐
培养细胞的生物学特性检查
细胞因子检测方法现状
培养基的制备
PAS染色法
细胞凋亡过程中c-erbB-2基因的表达
细胞周期（Cell cycle）
流感病毒细胞分离培养
为什么大多数细胞培养需要用二氧化碳培养箱
磷酸钙法转染HEK293T细胞
细胞计数及活力测试操作步骤
组织培养基母液的配制
细胞凋亡蛋白在肝癌发生过程中的作用
Mouse B cell isolation with magnetic beads
细胞骨架的光学显微镜观察
细胞器的光镜切片和电镜照片观察
原代细胞的概述
明胶
组织培养绪论和基本理论知识
培养细胞的生物学特性
液泡系的活体染色及电镜照片观察
电子显微镜[electron microscope]
角质形成细胞的原代培养方法
血小板功能检测在临床中的应用
正常大兔脑微血管内皮细胞的培养
MTT测定CMC
神经干动作电位实验
细胞组分的化学反应实验
构建多元化的细胞生物学PBL教学模式
MEF小鼠胚胎成纤维细胞知识总结
动物胃黏膜上皮细胞的培养
动植物细胞培养
孚尔根反应（Feulgen Reaction）
Grasshopper Spermatogenesis
细胞骨架系统的显示和观察
不连续密度梯度分离法分离NK细胞
动物子宫颈部上皮细胞的培养
IFN-α逆转白血病细胞耐药性的实验
肾血管支的分离和血管平滑肌细胞培养
FCM 医学生物学应用和进展
巨噬细胞的分离与纯化
ABi病毒
PEG介导的动物细胞融合(cell fusion)技术
细胞培养:特殊培养法
内分泌干扰物诱导睾丸生精细胞凋亡的研究进展
肌组织细胞培养
细胞冻存、解冻方法以及细胞计数
巨噬细胞吞噬现象的观察
染色体制备体会
细胞培养技术细胞生物学应用
肿瘤细胞培养方法
微粒体的分离
细胞培养（cell culture）概述
人外周血淋巴细胞培养
应用于活细胞成像的一次性细胞培养芯片
NAG检测法检测细胞生长
培养细胞的冻存及复苏
监控丙型肝炎病毒感染新技术
体内细胞培养及其操作步骤
细胞增殖的检验方法-酸性磷酸酶法
细胞凝集反应
FITC标记抗体-改良法
体细胞胚诱导
人的外周血淋巴细胞培养实验
人或动物肺毛细血管原代内皮细胞的分离
细胞分析术快讯
细胞周期基础知识
叶绿体的分离与荧光观察
细胞生物学名词解释（2）
细胞质
组织培养细胞生物学
细胞及亚细胞组分的分离
细胞培养技术在肿瘤方面的研究
细胞的基本结构
细胞生物学实验技术
细胞生物学历史与展望
细胞培养中边缘效应和解决办法
实用哺乳动物细胞培养手册（下）
动物细胞培养及外源基因的导入实验
实用哺乳动物细胞培养手册（上）
实用哺乳动物细胞培养手册（中）
细胞膜
293细胞
细胞核的分离
植物原生质体的分离、融合和培养
内皮细胞培养
肝细胞培养
原代细胞富尔根（Feulgen）反应显示DNA
细胞的冷冻保存与复苏技术
重组人TFAR19蛋白对羟基喜树碱诱导人7721肝癌细胞凋亡的增敏作用
氯化铯密度梯度离心纯化轮状病毒
悬浮细胞与贴壁细胞的冻存与复苏
用支持物培养法培养贴壁细胞
细胞介导细胞毒作用检测法-荧光法检测CMC
红细胞计数
Blood Cells: A Practical Guide
Timing of Cycles
染色质小分子多肽的提取
细胞生物学实验教学改革探索
[激光显微细胞分离技术]
肿瘤细胞的标记及其活体荧光成像
细胞表面抗原染色的组织样本制备方法
各细胞系生物学特性汇总
人真皮复合培养法
线立体DNA的快速制备
生物技术干细胞工程
体内可见光成像技术用于干细胞研究
细胞衰老机制学说
弹性蛋白的分离
研究T细胞产生的细胞因子的3种方法
昆虫细胞的培养液适应[4阶段]
原代细胞培养实验
单克隆抗体细胞融合实验
Ascaris Oogenesis
原代悬浮细胞吉姆萨染色法
细胞损伤模型
细胞的衰老
树突状细胞
乳腺组织培养
H9C2心肌细胞系的特性
细胞凝集反应的实验原理和方法
透射电子显微镜的原理与演示
细胞培养的基本技术
植物多倍体诱导及其细胞学鉴定
动脉平滑肌细胞 (Arterial smooth muscle cells)
正常小鼠原代破骨细胞培养方案
人角质形成细胞的分离培养
正常人包皮成纤维细胞的培养
正常人乳腺上皮细胞培养
正常角膜内皮细胞培养
细胞学技术
动物肠黏膜上皮细胞的培养
正常视网膜色素上皮细胞原代培养
酸性磷酸酶（ACP）的显示
动物组织肿瘤细胞
基因操控制造人体胚胎干细胞将不再困难
正常人食管黏膜上皮细胞培养
正常人关节软骨细胞的长期培养
正常人骨膜内成骨细胞原代培养
树突细胞的分离-用小鼠骨髓前体细胞培养树突细胞
利用FFNs观察单个突触前端多巴胺的释放
外周血干细胞移植简介
溶酶体的分离
细胞凋亡检测-生化特征的检测方法
细胞凋亡检测方法
苏木精-伊红（HE）染色
原代贴壁细胞吉姆萨染色法
正常大鼠前脂肪细胞的培养
收到细胞株包裹后的细胞活化
细胞培养基种类与基本成分
羊水细胞培养
细胞冷冻和复苏[2]
细胞工程基础实验技术
影响转染实验的因素
原代细胞甲基绿-哌咯宁法显示DNA和RNA
制备高效率感受态
线立体的分离
荧光探针标记法测定细胞膜流动性
减数分裂实验原理和步骤
Mechanisms of Lymphocyte Activation and Immune Regulation XI: B Cell Biology
成人骨髓基质细胞体外培养及鉴定
跨膜运输
Nature Medicine：诱导细胞重编程新方法
细胞介导细胞毒作用检测法-单细胞CMC试验
冷冻细胞活化
酵母表达系统
细胞培养基础篇
小鼠单个核细胞分离一步梯度法去除死细胞
细胞内糖类染色方法—过碘酸席夫反应法
原代细胞DNA与RNA的吖啶橙荧光染色法
FITC标记抗体-Marsshall氏法
PriCells: 磁珠法分离小鼠T细胞和B细胞
细胞介导细胞毒作用检测法-[3H]脱氧胸苷释放法
移液器的正确操作和注意事项
293/293T细胞
原核细胞和真核细胞的演化过程
细胞系或细胞株的建立
细胞系的建立
革兰氏染色法(原理、方法和意义)
动物细胞培养技术（大规模培养、灌注培养、无血清培养）
干细胞老化的表现和处理
干细胞的“完全手册”
密度梯度离心基础
肾小球分离、移植培养
FITC标记抗体-Chadwick氏法
细胞培养常见问题及其解决方法
甲醇酵母细胞表达系统
细胞器的分离实验
实验：细胞显微注射技术
细胞内功能区隔与蛋白质分选
用多聚甲醛固定细胞爬片、甩片或切片
推荐：染色体的标本制作及其组型实验
PriCells: 动物小血管内皮细胞的分离
一次性细胞培养皿等标明Tissue Culture Treated是什么意思
磁化细胞分离器分离法（MACS）分离NK细胞
细胞骨架
细胞培养从头学起
细胞转染技术原理及应用（瞬时转染和稳定转染）
层粘连蛋白（Laminin，LN）的分离
细胞周期
细胞重量测定技术
细胞培养实验
Basic Fluorescent in situ Hybridization (FISH)
免疫磁性微珠分离法分离T淋巴细胞和B淋巴细胞
细胞划痕实验
台盼蓝染色法
细胞因子的生物活性检测法
补体的生物学功能
细胞的显微与亚显微结构观察
哺乳动物细胞表达系统
用荧光激活的细胞分类仪检测调亡细胞
I125相关问题总结
正常视网膜米勒细胞原代培养
细胞培养抗生素
细胞外基质
几种转染方法的比较：DEAE葡聚糖、磷酸钙共沉淀法、脂质体法、电穿孔法
细胞凋亡与疾病
吖啶橙/溴化乙锭双荧光染色
牛或猪大血管原代内皮细胞的分离-消化刮脱法
Cell Culture Procedure
实用临床麻醉学上下册北京科技出版社 2017
翟中和《细胞生物学》（第4版）笔记和课后习题（含考研真题）详解
现代麻醉学第五版（第5版）上下共两册精装邓小明，姚尚龙主编人民卫生出版社正版临床医生必读
摩根临床麻醉学第6版北京大学医学出版社 2020年
Morgan & Mikhail’s Clinical Anesthesiology《摩根临床麻醉学》（第5版）
动物细胞培养—基本技术和特殊应用指南第7版章静波译l 2019-完美版
细胞生物学发展史
胚胎干细胞有何研究意义
杂交瘤技术（实验原理、用品、方法）
磁珠分选柱手工操作分离小鼠CD4+ CD25+抑制性细胞
植物脱毒
原生质体分离与体细胞杂交
细胞悬浮系的建立与种细胞筛选
2007年工具书Cell Biology, 2nd ed (Pollard Earnshaw)
血清热灭活―浪费时间？
细胞生物学名词解释（1）
细胞问题解答集锦
细胞线粒体提取方法
巨噬细胞移动抑制因子活性机制与炎症性疾病
干细胞的分类
细胞中的微丝染色及微丝与细胞形态的观察实验
正常小梁细胞原代培养
细胞生长的ATP检测
线粒体肿胀的检测方法
利用细胞学标本单个分散细胞的优势制作细胞蜡块
红细胞
电穿孔和电融合技术
细胞增殖的检验方法-结晶紫法
白细胞计数
阿司匹林对人γδT细胞杀伤消化系统肿瘤细胞的影响
磷酸钙细胞转染技术
线粒体和液泡系的超活染色与观察
细胞培养基本技术（免疫细胞分离、肿瘤细胞株传代培养）
补体系统（缺陷）与疾病
Hemacytometer Reference Guide
Cell counting with an hemacytometer.
血细胞计数及大小测量
一株抗人内皮细胞培养及单克隆抗体的制备与鉴定方法
毕氏酵母感受态细胞制备及转化
肝脏的组织化学
细胞培养常用设备及实验技巧
正常细胞和肿瘤细胞常规核型标本制备
DNA荧光法染色检测支原体试剂盒
经典参考书《Cell Biology: A Laboratory Handbook》
昆虫细胞：高MOI杆病毒感染
组织培养细胞染色体制备
CHO细胞株的部分特性
细胞培养[cell culture]
细胞培养在临床诊断中应用
正常兔食管上皮细胞培养
流式检测细胞周期
原代细胞生长的基本体系
细胞凋亡的机制
推荐：肿瘤细胞的培养
DMEM细胞培养基的成分
细胞诊断要诀
细胞生长检测:染料染色法测定细胞数
供体-受体原生质体融合与胞质杂种的形成
毛细血管内皮细胞培养
线粒体的分离与观察
致癌化学物转化细胞：转化叙利亚地鼠胚胎细胞实验
细胞培养无菌操作基本技术
Transfecting Suspension Cells(悬浮细胞转染)
上皮细胞培养技术
胶原蛋白的分离
细胞融合法提高HBsAg表达量方法研究
Carcinosarcome Walker 256特性
豚鼠耳蜗微血管内皮细胞的分离培养与鉴定
MTT Cell Proliferation Assay
骨肉瘤细胞UMR106
正常关节软骨细胞的短期培养
核酸(DNA和RNA)的细胞核定位观察
原体污染后的治疗与处理
光学显微镜的使用与细胞形态观察
L-02细胞系特性
MAPK信号转导通路与疼痛敏化调控研究进展
大规模培养技术的操作方式
原代心肌细胞培养方法探讨
p19cl6细胞
细胞质流动及其影响因素
细胞活性测定方法专题讨论
动物细胞大规模培养技术
Cycling Tubulin
应用细胞融合技术制备染色体提前凝集标本
血涂片的制备和细胞大小的测量
Making Microtubule Seeds
Dissociation of Cells from Primary Tissue
细胞融合与单株抗体
picking cloned cells-挑克隆
BrdU标记法-细胞增殖的检验方法
DNA显示和细胞有丝分裂相的观察实验
动物组织正常及及永生化细胞
细胞学技术概述及注意事项
人类组织肿瘤细胞
正常大兔骨间充质前体细胞的体外成软骨培养
流式细胞术检测细胞凋亡
原代细胞骨架的染色方法
BAF CELL的特性和用途
实验室常用细胞株
LAK(Lymphokine activated killer cell)细胞的培养
上海细胞库
用DAPI进行细胞染色
中国科学院细胞库可供应细胞目录
血球计数板计数误差的来源及解决方案 ——自动化计数
PBMC 细胞的精确计数和活性分析
白细胞计数参考值[WBC]
Human Embryonic Stem (ES) Cell Protocols――Splitting Human ES cells on Matrige
核孔复合体
Laboratory Methods in the Study of Endometrial Claudin-4
Tools to Investigate ROS Sensitive Signalling Proteins
蛋白质检测－Western Blot
Rule-Based Modeling of Signal Transduction: A Primer
Epitope Mapping
Techniques to Measure Nucleic Acid-Protein Binding and Specificity: Nuclear Extract Preparations, DN
Immunoelectron Microscopy of Cryofixed Freeze-Substituted Mammalian Tissue Culture Cells
Transcriptome-Based Identification of Candidate Membrane Proteins
Sequence-Specific Primed PCR (PCR-SSP) Typing of HLA Class I and Class II Alleles
Transcriptome-Based Identification of Candidate Membrane Proteins
Sequence-Specific Primed PCR (PCR-SSP) Typing of HLA Class I and Class II Alleles
Treatment of Spontaneous Soft Tissue Sarcomas in Cat
侧群干细胞（sp细胞）的分离与分选
转铁蛋白(transferrin)
内分泌信号(endocrine signaling)
转胞吞作用(transcytosis)
显微镜放射自显影样品的制备及观察
Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells
Confirmation of the Presence of Drugs of Abuse in Urine
小分子RNA(small RNA)
Thin-Layer Gel Filtration: Determination of Protein Molecular Weights
Oocyte Extracts for the Study of Meiotic M-M Transition
胚胎干细胞培养操作规程
Rockland Dr.Moncada 分享：如何获得更漂亮的流式结果
Imaging Hematopoietic Stem Cells in the Marrow of Long Bones In Vivo
In Situ Hybridization of Whole-Mount Embryos
Autoradiography of 1-and 2-Adrenoceptors
胚胎干细胞培养
肌动蛋白(actin)
A Mammalian Cell-Based Assay for Screening Inhibitors of RNA Cleavage
细胞培养中血清使用的常见问题
Amnis公司的ImageStreamX图像流式细胞仪
血细胞的细胞化学染色
核仁
周期蛋白依赖性蛋白激酶(cyclin-dependent protein kinases, Cdks)
Granzyme A、B介导的凋亡信号通路
真核细胞(eucaryotic cell)
Collagen gel containing 3T3 fibroblasts (dermal equivalent for raft culture)
C带(C-banding)
Multicolor BiFC Analysis of G Protein Complex Formation and Localization
荧光检测细胞支原体污染
Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen
载脂蛋白(apolipoprotein B)
Fluorescence Techniques for Studying Membrane Transport Proteins: The P-Glycoprotein Multidrug Trans
Cell-Scatter Assay
Cytoskeletal-Antigen Specific Immunoliposomes: Preservation of Myocardial Viability
MTT的替代产品—XTT，WST-1，CCK-8（WST-8）
叶绿体被膜(membrane envelope)
光系统(photosystem)
内共生学说(endosymbiont hypothesis)
培养细胞染色体显色法
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
Simplified Calcium Phosphate Coprecipitation
肽酰转移酶(peptidyl transferase)
MudPIT Analysis: Application to Human Heart Tissue
Assays to Measure Stress-Activated MAPK Activity
Classical Human Epidermal Keratinocyte Cell Culture
MTS检测细胞生长
Measurements of White Adipose Tissue Metabolism by Microdialysis Technique
Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis
In Vitro Matrigel Angiogenesis Assays
Locked Nucleic Acid Aptamers
核仁组织区（nucleolus organiting region，NOR）
原核融合(pronuclei fussion)
分裂间期
细胞遗传学――原位杂交(ISH)
Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis Assay
激光显微细胞分离技术
Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damage
Keratinocyte Transient Transfections
Quantitation of Sirolimus Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS)
Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry
细胞生物基本方法：常规组织培养法
Well-Differentiated Human Airway Epithelial Cell Cultures
Deep Sequencing of Small Chromatin-Associated RNA: Isolation and Library Preparation
多聚核糖体(polyribosomes)
Over the past few years, the view that little or no cell turnover or replacement takes place within
Cultures of Stem Cells of the Central Nervous System
CELL ADHESION
EB Differentiation
胞内运输(intracellular transport)
支原体检测：DNA萤光染色法
宿根福禄考组织培养及快繁技术
PAIR Technology: Exon-Specific RNA-Binding Protein Isolation in Live Cells
细菌培养倒平板的小技巧
In Situ Immunofluorescence Analysis: Immunofluorescence Microscopy
常用细胞凋亡检测方法（三）
GTP结合蛋白(GTP binding protein, G蛋白)
MSCs的分离
染色体骨架(scaffold)
原位末端转移酶标记技术检测凋亡
Cell Quantification
细胞培养实验中常用器材和试剂的配制
凝集素(lectin)
M6P受体蛋白(M6P receptor protein)
Gas Chromatography-Mass Spectrometry Method for the Determination of Methadone and 2-Ethylidene-1,5-
Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interaction
Post-translational Modification of p53 by Ubiquitin
Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
293-T细胞培养实验中注意事项
Ribonuclease Protection Analysis of Gene Expression in Xenopus
Observational Study Design
Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices
Immobilization of Enzymes on Thermo-Responsive Polymers
Analysis of Rho GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy
Maintenance of Cell Culture
Cell Lineage Analysis: X-Inactivation Mosaics
Targeting Repair Proteins to the Mitochondria of Mammalian Cells Through Stable Transfection, Transi
Electroporation of Antigen-Presenting Cells for T-Cell Recognition and Cytotoxic T-Lymphocyte Primin
Cell Grafting and Fate Mapping of the Early-Somite-Stage Mouse Embryo
Renal and Cardiac Na+K+-ATPase and Aconitase in a Rat Model of Fetal Programming
酵母菌落直接质粒转化法
Generating Tamoxifen-Inducible Cre Alleles to Investigate Myogenesis in Mice
G418筛选慢病毒感染稳定细胞株
Colony Forming Unit Assays for MSCs
低毒性X-tremeGENE DNA转染检测
Biochemical Isolation of mtDNA Nucleoids from Animal Cells
向导RNA(guide RNA, gRNAs)
受体( receptor)
啤酒酵母菌营养缺陷型菌株的筛选
微粒体(microsomes)
Capillary Electrophoresis With Laser-Induced Fluorescence: Environmental Applications
成体干细胞(somatic stem cell)
大蒜细胞SOD的提取与沉淀分离
Regulation of AP-1 Activity by TGF
细胞培养级别二甲基亚砜DMSO
Isolation and Culture of Human Macrophages
真核细胞转染操作步骤
ABI推出的第一台声波聚焦流式细胞仪Attune™
如何添加MEM培养基中补充成分
细胞融合(cell fusion)
钙粘着蛋白(cadherin)
Anionic pH Sensitive Lipoplexes
细胞衰老(cellular aging，cell senescence)
Cardiac Morphogenesis and Dysmorphogenesis I. Normal Development
The cDNA Sequencing Project
血清保存时必须注意的事项
细胞组分的分析方法――线粒体的分离与观察
发表SCI论文的一些小技巧
Cell-Spreading Assays
细胞膜受体
人造血干细胞扩增的研究进展
PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE
植物体内可溶性糖含量的测定(蒽酮法)
BrdU标记法检测细胞增殖
O-连接的糖基化(O-linked glycosylation)
Immunofluorescence Analysis Using Epitope-Tagged Proteins: In Vitro System
流式细胞仪检测细胞凋亡――Annexin V/PI双染色法
T and B cell Isolation
内吞作用
交叉
Measurement of Agonist-Stimulated [35S]GTPγS Binding to Assess Total G-Protein and Gα-Subtype-Specif
信号传导
细胞培养常用的溶液（之一）——消化液
染色体显微切割及新进展
细胞介导细胞毒作用检测法3：LDH释放法
细胞介导细胞毒作用检测法6：荧光法检测CMC
MEDIA FOR EMBRYO CULTURE AND MANIPULATION
中膜体(mesosome)
血清使用知识点介绍
线粒体的活体染色及电镜照片观察
Stewart Laboratory Standard Operating Procedure
Identification of Homing Peptides Using the In Vivo Phage Display Technology
对控基因(pair-rule genes)
细胞膜的通透性观察实验原理和操作步骤
脂肪酸的β-氧化
细胞色素C的制备及含量、活力测定
脂肪干细胞（ASCs）的提取及鉴定
结晶紫法检测细胞增殖
细胞培养中支原体污染的特点及检验的笔记
秋水仙素(colchicine)
组织切片的原位杂交细胞化学方法
Extraction of Plant RNA
Differential Display Using Random Hexamer-Primed cDNA, Motif Primers, and Agarose Gel Electrophoresi
检测细胞凋亡的几种方法
多线染色体
核被膜(nuclear envelope)
Activity Assays for Extracellular Signal-Regulated Kinase 5
细胞增殖与毒性检测：RTCA技术法
Adipose Stem Cell Differentiation into Smooth Muscle Cells
大鼠辛型肝炎病毒（TTV)酶联免疫分析（ELISA）
细胞固定、染色原理与方法
Assessment of Urinary Pheromone Discrimination, Partner Preference, and Mating Behaviors in Female M
细胞组分和细胞器――细胞器分离
细胞吸附试验
核仁（nucleolus）
流式细胞仪高级技巧（Purdue）
Transwell侵袭实验总结之操作步骤
一氧化氮（NO）信号通路研究
细胞培养技术常见问题解答
TISSUE CULTURE STOCK SOLUTIONS AND MEDIA
信号级联放大(signaling cascade)
TUNEL 方法
染色体核型分析实验
ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
光脱色荧光恢复技术(fluorescence recovery after photobleaching FRAP)
A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidi
白细胞的吞噬作用
血细胞的分离方法（中性粒细胞、嗜碱性粒细胞
荧光标记法检测细胞凋亡的操作步骤
细胞计数和台盼蓝染色
菌体/细胞裂解法汇总
抗血清保存的三种方法
Mapping the Regions of RNase P Catalytic RNA That Are Potentially in Close Contact With Its Protein
动物细胞转染方法
Large Scale Tubulin Preparation
Assays for Human DNase I Activity in Biological Matrices
Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryoni
STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination
Cell-Surface ProteinProtein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies
Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood
Serum Lamotrigine Analysis
Generation, Use, and Validation of Receptor-Selective Antibodies
细胞膜结构的物质组成及作用
CHO Centrosome Prep:
特异性免疫功能
Calcium Imaging in Cell-Cell Signaling
人中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫分析
食蟹猴白细胞介素-2（IL-2）酶联免疫分析（ELISA）
FISHing for Cytokines: Methodology Combining Flow Cytometry and In Situ Hybridization
精母细胞（spermatocyte）
药物/毒物作用后的细胞数量测定
Expression of Human Collagenase I (MMP-1) andTIMP-1 in a Baculovirus-Based Expression System
用尼龙毛法分离B细胞实验
几种转染方法对比
细胞株HLA-A*02检测方法
Carboxypeptidase Y (EC 3.4.16.1)
细胞培养中污染的清除与预防
细胞周期的测定原理与操作步骤
血型糖蛋白(glycophorin )
Construction of Linker-Scanning Mutations by Oligonucleotide Ligation
细胞裂解液（Cell Lysis Solution）的配置方法
自行配制DMEM的方法
微载体培养技术（microcarrier culture technique）
酵母菌杂交
siRNA转染的相关问题解答
$载体做共转染时的复制起点\启动子问题$
载体做共转染时的复制起点\启动子问题
罗氏公司新推出两款高性能的DNA转染试剂
A位点(A site)
Percoll
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Electrophoretic Mobility Shift
载体
流式细胞仪技术专辑
Adaptive Treg Generation by DCs and Their Functional Analysis
Purification of human mononuclear cells and neutrophils
Power and Sample Size
HepG2转染的方法讨论
[3H]脱氧胸苷摄入细胞计数
TEM Visualization of Microtubules
HBSS的配制和使用攻略
配制RPMI1640培养基13点需知
Preparation of Dual-Color Polarity-Marked Fluorescent Microtubule Seeds
腺病毒感染过程
微管蛋白(tubulin)
支持物培养法培养贴壁细胞
动物细胞培养技术及hanks液的配制
α螺旋-转角-α螺旋基元(helix-turn-helix motif)
减数分裂过程的识别
Sperm Chromatin Structure Assay (SCSA)
细胞器的光镜切片与电镜照片观察
Cell Cycle Staining Protocol-DAPI
Measurement of the GTPase Activity of Signal-Transducing G-Proteins in Neuronal Membranes
猪白细胞介素-12（IL-12）酶联免疫分析（ELISA）
最小化非特异性染色方法
In Situ Immunofluorescence Analysis: Analyzing RNA Synthesis by 5-Bromouridine-5-Triphosphate Labeli
乳鼠心肌细胞的微血管内皮细胞培养
全球CO2培养箱生产商网站
线粒体基质(matrix)
癌基因(oncogene)
流式细胞术在高等植物研究中的应用
细胞生长检测1：[3H]脱氧胸苷摄入法测定细胞数
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyd
Transmigration of Human CD34+ Cells
Mass-Spectrometric Characterization of Phospholipids and Their Hydroperoxide Derivatives In Vivo: Ef
Tissue Culture of PtK1 Cells
Millipore独家推出表皮角质形成细胞的3D培养基
Method: Lymphoblastoid Cell Lines from Frozen Whole Blood
src基因(sarcoma gene)
Invitrogen推出首个间充质干细胞的无血清培养基
植物多倍体鉴定技术
Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-
溶酶体与细胞凋亡
细胞被(cell coat)
昆虫细胞的培养液适应
细胞凋亡检测
半纤维素(hemicellulose)
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
FRET Imaging of Cells Transfected with siRNA/Liposome Complexes
细胞培养基的选择及常用数据库
Trypan blue viability test
T细胞分离用尼龙毛柱
细胞转染成功与否的八大要素
人脐静脉内皮细胞的体外培养、鉴定及形态观察
The Use of P-Element Transposons to Generate Transgenic Flies
光合磷酸化(photophosphorylation)
细胞色素(cytochromes)
细胞培养中常用培养基及基本特性
利用细胞计数板测定血细胞数量及大小
线粒体凋亡的信号通路图
转移(metastasis)
培养基母液的配置注意事项
Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal S
Akt/Pkb信号通路图
细胞同步化
昆虫细胞的低MOI杆病毒感染
细胞凋亡早期检测
Fas/Fas L信号通路图
细胞培养――细胞保藏
高尔基体（Golgi apparatus，Golgi complex）
细胞凋亡的3种途径是什么？
超净台工作原理及使用方法
Rapid Identification of Unknown Heteroplasmic Mitochondrial DNA Mutations with Mismatch-Specific Sur
分化抑制（differential inhibition）
细胞计数及活力测定方法
当冻存管储存在液氮液相中而没有使用CryoFlex T
Biological coating procedures for glass coverslips
美国LONZA无血清培养基技术及产品介绍
Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes
您所不知道的Treg——“NAD+诱导细胞凋亡”途径
神经细胞培养大全
细胞凋亡晚期检测
Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6)
流动镶嵌模型
BD发布新一代流式细胞仪FACSAria III细胞分选系统
Signal Amplification for DNA and mRNA
流式细胞实验方法操作总结
细胞培养用溶液的配制与消毒实验方法
双链断裂重组模型（model of double-strand breaks recombination）
差别基因表达(differential gene expression)
肿瘤细胞培养之成纤维细胞的消除
Detection of BrdU Incorporation in DNA Synthesizing Cells
5S rRNA基因(5S rRNAgene)
微纤维体系
紧跟诺贝尔奖热点：锐博生物解析囊泡“exosome”重大发现
Mouse Models of MMP and TIMP Function
离子霉素的基本信息和使用需知
Statistical Methods in G-Protein-Coupled Receptor Research
Tubulin Polymerization with GTP/GMPCPP/Taxol
促进扩散(facilitated diffusion)
Detection of Apoptosis in Paraffin-embedded tissues by ISEL (In Situ End Labeling)
细胞化学
细胞增殖周期
细胞膜的基本化学组成及跨膜物质运输
氰化高铁血红蛋白测定法及红细胞计数的方法与注意事项
达人推荐：23条多聚赖氨酸实验的经验分享
什么是MTT，什么是MTT法
灯刷染色体(lampbrush chromosome)
人类体细胞染色体标本制备与核型分析
微体
细胞解冻的实验流程和几点需知
酵母遗传学方法8：酵母活体染色
p38 MAPK信号通路图
JNK/SAPK信号通路级联图
动物细胞培养中Hanks液、D-Hanks液和消化液的配制方法
Immunoassays for Collagenase-Mediated Cleavage of Type I and II Collagens
细胞膜渗透性实验原理与操作步骤
细胞组分的分析方法――定量细胞化学分析技术
HEK-293 293A 293S 293T 293FT区别比较
IFN
小GTP结合蛋白(small GTP binding protein)
反式作用因子(trans-acting factor)
Alkaline phosphatase staining
一个信号两种功能
Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein That Provides a Cell
人类X染色质的观察
Western Blot实验中转膜和封闭的注意事项
MTT所有问题大汇总
BrdU标记法
用MTS检测细胞生长
细胞裂解液的配置方法
细胞介导细胞毒作用检测法4：时间分辩荧光分析法
Analysis of Multisite Phosphorylation of the p53 Tumor-Suppressor Protein byTryptic Phosphopeptide M
植物原生质体融合
Calmodulin-Tagged Phage and Two-Filter Sandwich Assays for the Identification of Enzymatic Activitie
驱动蛋白(kinesins)
ICBR Flow Cytometry Core Laboratory Paraformaldehyde Fixation of Cells
表面受体(surface receptor)
The Cobblestone-Area-Forming Cell Assay
光反应(light reaction)
抑癌基因(Tumor suppressor gene)
受体介导的内吞作用(receptor-mediated endocytosis)
HEPES缓冲液的配制和使用方法
Thawing Cells (Schreiber protocol)
细胞培养室的建立
Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
DNA疫苗实验方法
自由扩散
Tips for Tissue Culture
Akt信号通路总况
Classical and Molecular Cytogenetic Analysis
外周血培养基配制
Isoelectric Focusing (IEF) of Proteins in Thin-Layer Polyacrylamide Gels
真核细胞蛋白表达疑难求解!
Master Cell Bank
4小时51Cr释放法检测CMC活性
The Generation of Multicopy Recombinant Strains
真细菌(Bacteria, eubacteria)
T细胞亚群的分离
DNA Polymerases (EC 2.7.7.7)
扩散(diffusion)
Measurement of Mitochondrial ROS Production
Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells
Working with a Statistician
人白细胞介素7（IL-7）酶联免疫分析（ELISA）
The BAL 31 Nucleases (EC 3.1.11)
大鼠碱性磷酸酶(ALP)酶联免疫分析（ELISA）
酵母遗传学方法7：随机孢子分析
TUNEL法检测细胞凋亡的实验原理、试剂配制及操作步骤
293T细胞的培养与传代心得
大肠杆菌营养缺陷型菌株的筛选
肌组织培养
染色体CBG标本制备
II型内含子(group II introns)
Dorsal Root Ganglion Neuron Culture from Adult Rats
孚尔根[Feulgen]核反应染色法
细胞生长检测3：XTT检测法
Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach
Lentiviral Vector Engineering for Anti-HIV RNAi Gene Therapy
小鼠胚胎成纤维细胞MEF培养相关知识总结
紧密连接
Staining Methods for cell death Z. Xia 10/2/95
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections
Online Tools for Predicting Integral Membrane Proteins
核酸酶保护分析简介
Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipase D
转基因――材料和试剂
细胞凋亡物质释放的反馈调节
RNA-Dependent RNA Switches in Bacteria
研究细胞凋亡的意义
用于融合试验的主要骨髓瘤细胞系
贴壁细胞的消化方法介绍
Quantitation of Murine and Human Hematopoietic Stem Cells by Limiting-Dilution Analysis in Competiti
叶绿体(chloroplast)
装配反应因子(assembly reaction factor, ARF)
General Guide for Cryogenically Storing Animal Cell Cultures
CEN 序列(centromeric sequence)
微碳纤电极与细胞量子化分泌的记录方法
黄素蛋白(flavoproteins)
MTT法检测细胞生长
N-端规则(N-end rule)
卵子发生(oogenesis)
离子通道偶联受体(ino-channel linked receptor)
2%多聚甲醛固定剂配方二则
细胞培养环境
纤维粘连蛋白(fibronectin, FN)
Methods for Determining Myc-Induced Apoptosis
组蛋白(histone)
人细胞间粘附分子1(ICAM-1)酶联免疫分析（ELISA）
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion
Detection of Replication Competent Retrovirus and Lentivirus
Reactive Oxygen Species Production by Mitochondria
Using the Textpresso Site-Specific Recombinases Web Server to Identify Cre Expressing Mouse Strains
细胞学说
细胞凋亡如何检测？
培养基的配制和灭菌
细胞裂解实验方法总结
培养乳鼠心肌细胞的缺氧/复氧(A/R)损伤模型和缺氧预处理(APC)模型
细胞冻存的程序降温盒的使用注意事项
Strategies to Generate Induced Pluripotent Stem Cells
免疫酶标技术
基因转染之磷酸钙-DNA共沉淀技术
AAV-293细胞的传代心得
Isolation of Primary Fibroblasts from Mouse Embryos
GOLGI-VESICLE PREPARATION FROM PEA HYPOCOTYLS
自噬作用(autophagy)
Hanging drop aggregation assay
昆虫细胞--高MOI杆病毒感染
Isolation and Differentiation of Amnion-Derived Stem Cells (ADSCs)
母体基因(maternal gene)
细线期(leptotene stage)
翻译后转运(post-translational translocation)
Solutions for Tissue/Cell Digestion
流式细胞仪结构与原理
脂质体介导的稳定转染
表皮生长因子(epidermal growth factor, EGF)
酶联受体(enzyme linked receptor)
Actin Capture Assay
Protein-Protein Interactions in Signaling Cascades
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
细胞计数
非循环式电子传递途径(noncyclic electron transfer pathway)
细胞培养基本参数和基础知识解答
细胞坏死和凋亡的形态变化
Peptide Aptamers for Small Molecule Drug Discovery
大鼠氨基脲敏感性胺氧化酶(SSAO)酶联免疫分析（ELISA）
星形胶质细胞的一些经验
MEM培养基的配制步骤
染色体结构显示和检测
Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis
SDS-PAGE凝胶的制备过程
MAPK信号通路介绍
细胞爬片实验经验总结
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
微丝（microfilament）
SDS Polyacrylamide Gel Electrophoresis of Proteins: Determination of Protein Molecular Weights and H
如何得到稳定转染的细胞
流式细胞仪原理及及其在植物上的应用和选用
Recombinant Collagen Trimers from Insect Cells and Yeast
细胞连接(cell junction)
Adipocytes
脂质体(liposome)
细胞内受体(intracellular receptor)
动物细胞培养技术（大规模培养、灌注培养、无
Quantitation of Morphine, Codeine, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone, and 6-Monoace
Slice and Explant Culture Protocols Hevner lab 2002
Method: Cell Counts Using a Hemacytometer
Use of the B.D. FACS or Calibur Flow Cytometers
Isolation of Resident Cardiac Progenitor Cells by Hoechst 33342 Staining
水通道蛋白(aquaporin)
细胞染色制片方法
如何进行血细胞的分离实验
细菌原生质体融合
果蝇细胞的培养实验方法（Culturing Drosophila cells)
MTT的两个用途及原理
In-Vitro Adipocytes Differentiation
翻转酶(flippase)
原代细胞的培养与建系
细胞培养血清
双向琼脂扩散试验（Double immunodiffusion test）
alpha-MEM培养基成分及其与MEM培养基的比较
Genetic Cell Ablation
Determination of IC50
人体间期细胞X―小体的制备
Fluidigm推出首个单细胞捕获和制备的平台
Testing for Bacteria and Fungi
细胞膜的凋亡检测的基础原理
A rapid, quantitative and inexpensive method for detecting apoptosis by flow cytometry in transientl
Coupling of Dephosphorylation and Nuclear Export of Smads in TGF- Signaling
膜结合细胞器(membrane-bound organelles)
DMEM培养基中DMEM粉剂配制方法步骤
Human Placental Alkaline Phosphatase Stain
CD3/ CD4 /CD8 T cell subset counts
染色体扫描电镜标本制备及观察
RPMI-1640(A) 细胞培养基（粉末型）成分
ELISA法检测细胞凋亡
MTT实验最全指南
核小体（nucleosome）
细胞编程性死亡
Affinity-Based Assay of Rho Guanosine Triphosphatase Activation
基膜(basal lamina，basement membrane)
Protocol for Cell Fusion
线粒体(mitochondrion)
Measurement of Mitochondrial DNA Copy Number
核蛋白(nuclear protein)
活细胞培养观察方法
Evaluation of Rapamycin-Induced Cell Death
内质网与细胞凋亡
离心技术与叶绿体的分离
MAPK ERK Pathway
大鼠核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫分析
16sDNA简介及其应用于细菌鉴定的优点
Positional Cloning
后期促进复合物(anaphase-promoting complex,APC)
细胞生长检测7：碱性磷酸酶检测法（AKP法）
Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cy
核酸酶超敏感位点(nuclease-supersensitive site)
细胞转染实验之如何做好脂质体介导DNA转染
动物细胞凋亡和植物细胞凋亡有区别吗？
条件突变体(conditional mutants)
Multifunctional CPP Polymer System for Tumor-Targeted pDNA and siRNA Delivery
Culture Systems: Embryo Co-Culture
293细胞的传代
协同运输(cotransport)
核质蛋白(nucleoplasmin)
表面受体超家族(surface receptor superfamilies)
干细胞实验技术入门
Cr Release Cytotoxicity assay
诱导多能干细胞iPSC培养基质如何转换注意事项
如何配制干粉培养基
Boyden Chamber Assay
细胞生长检测：MTS检测法
显微镜技术――荧光显微技术
联会复合体
Native Polytene Chromosomes of Drosophila melanogaster for Light and Electron Microscopic Observatio
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
Ras蛋白(Ras protein)
Working Cell Bank
冷冻蚀刻技术介绍
流式细胞术定量检测细胞凋亡的3种方法研究比较
Hanks液的配制方法步骤及其注意事项
Mesoderm Cell Development from ES Cells
Phospholipase A2 Activity
细胞染色常用染色试剂及细胞染色方法
外被体蛋白质Ⅰ(coatmer proteinⅠ, COPⅠ)
Claudin-4: Functional Studies Beyond the Tight Junction
细胞培养中死活细胞测定及细胞活力测定实验
放线菌（Actinomycete）
人B细胞活化因子（BAFF）酶联免疫分析（ELISA）
Tissue Culture Methods
Detection of Senescence-Associated Heterochromatin Foci (SAHF)
卵裂(cleavage)
天冬氨酸特异性半胱氨酸蛋白酶(cysteine-containing aspartate-specific proteases，caspase)
如何对细胞组分进行分级分离
人的外周血淋巴细胞培养及染色体标本制备
细胞冻存与复苏技术
裸小鼠移植瘤单细胞分离培养
EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/OOH and Immunospin-Trapping with Anti-DMPO
什么是bsa？bsa的分子量？
细胞生物基本方法：微生物污染的排除
Cryopreservation of Cell Lines
古细菌(archaebacteria)
七种常见细胞凋亡检测的方法与注意事项
经典信号通路之PI3K/AKT信号通路
Notch信号通路图(5张)
Analysis of Meiotic Recombination Products from Human Sperm
配体-门控通道(ligand gated channel)
第二信使(second messengers)
Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studies
胁迫门控通道(stretch-gated channel)
细胞凋亡的检测
CLIP: Crosslinking and ImmunoPrecipitation of In Vivo RNA Targets of RNA-Binding Proteins
缬氨霉素(valinomycin)
细胞粘着分子(cell adhesion molecule, CAM)
Generation of Human-Induced Pluripotent Stem Cells by Lentiviral Transduction
人类巴氏小体的观察
叶绿素(chlorophylls)
棉花室内考种
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
细胞质(cell plasma)
Determination of ERK Activity: Anti-phospho-ERK Antibodies and In Vitro Phosphorylation
选择最适合自己的3D培养系统
目的基因克隆的筛选与鉴定
人白细胞介素6(IL-6)酶联免疫分析（ELISA）
Immobilization of Enzymes by Bioaffinity Layering
Purification of Fibronectin from Human Plasma
顺式作用元件(cis-acting element)
细胞株培养细胞
电子自旋共振谱技术(electron spin-resonance spectroscopy,ESR)
用流式测细胞周期的一点总结
外周蛋白(peripheral protein)
Authentication of Cell Lines
Quantitative Analysis of Oxidized Guanine, 8-Oxoguanine, in Mitochondrial DNA by Immunofluorescence
细胞周期的DNA检测
Generation of a Bioengineered Tooth by Using a Three-Dimensional Cell Manipulation Method (Organ Ger
细胞计数及活力测定
Studying the Replicative Life Span of Yeast Cells
Kinetics of Riboswitch Regulation Studied By In Vitro Transcription
MEM培养基加入胎牛血清后出现沉淀是怎么回事？
Studies of Calmodulin-Dependent Regulation
HPLC-Based mRNA Differential Display
兔细胞间粘附分子-1(ICAM-1）酶联免疫分析（ELISA）
常规的琼脂糖凝胶电泳检测细胞凋亡
In Vitro Measurement of DNA Base Excision Repair in Isolated Mitochondria
细胞色素P-450(cytochrome P-450)
Capillary Electrophoresis for Pharmaceutical Analysis
胎牛血清使用中的常见问题
Annexin V/7-AAD Staining in Keratinocytes
复合物Ⅱ(complex Ⅱ)
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
红细胞沉降速率（erythrocyte sedimentation rate）
X和Y染色质标本的制作与观察
MTT实验前注意事项及贴壁细胞/悬浮细胞实验大汇总
细胞爬片的方法与心得
Global Analysis of Gene Expression by Differential Display: A Mathematical Model
成熟促进因子(maturation promoting factor，MPF)
Modeling Spatiotemporal Dynamics of Bacterial Populations
Imaging and Quantification of Recycled KATP Channels
NP40以及菌体/细胞裂解法
光系统Ⅱ(photosystem Ⅱ complex, PSⅡcomplex)
细胞冻存与复苏及细胞计数存活测试的操作方法与步骤
短杆菌肽A(gramicidin A)
细胞集落形成实验
细胞活性测定方法介绍和使用探讨
Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activ
效应物(effector)
植物有丝分裂临时制片(压片法)
ARF GTPase-Activating Protein 1
Strategies for Cloning Mammalian DNA Repair Genes
Assay for Antitumor and Lectin Activity in RNase Homologs
细胞凋亡的分子基础图
Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions
细胞凋亡过程中c
动物细胞培养中细胞的冻存与细胞复苏
细胞培养转染Wnt配体的生产和纯化
SH结构域(SH domain)
转染小分子RNAInvivofectamine 2.0和Neon系统
Establishment of Stable Transfectant of CHO Lec Cells
RTCA技术在肿瘤学中的应用（一） ——RTCA实时监测肿瘤细胞迁移及浸润
Isolation of T4 cells from xenograft tumors
通道蛋白(channel protein)
转染
Structural Probing of RNA Thermosensors
mRNA/siRNA转染技术及影响因素
内毒素激活巨噬细胞的信号传导
内收蛋白(adducin)
Polynucleotide Kinase (EC 2.7.1.78)
Preparing and using bacterial cells for electroporation
Immunocytochemistry is the most diffuse technique to visualize and localize specific biochemical com
Single and Double Colloidal Gold Labeling in Postembedding Immunoelectron Microscopy
细胞和细胞内含物的观察
细胞冻存复苏材料与方法步骤
G-Protein-Coupled Receptor Phosphorylation and Palmitoylation
细胞凋亡与坏死的区别以及细胞形态变化
人白细胞介素17（IL-17）酶联免疫分析（ELISA）
用淋巴细胞分离液分离单个核细胞
脂肪细胞凋亡与肥胖的关系
台盼蓝使用最佳浓度及时间是多少
细胞计数过程,用或者不用台盼蓝染色计数的区别
一种新型阿尔茨海默病动物模型的建立
Antisense Inhibition of Protein Synthesis and Function: Rabbit Retinal Protein
人抗中性粒细胞抗体（ANA)酶联免疫分析（ELISA）
Single-Cell Semiconductor Sequencing
微载体细胞培养法
Assaying NF-KB and AP-1 DNA-Binding and Transcriptional Activity
Gestation-Induced Uterine Vascular Remodeling
Histological Analysis of the Embryonic and Adult Tooth
原代培养与传代培养
Expressing Self-Incompatibility RNases (S-RNases) in Transgenic Plants
Neural Cell Counting: Cell Number Determination in Microplate Cultures
醌(uniquinone UQ)或辅酶Q(coenzyme Q)
细胞稳转建系后再转染遇到的问题
封闭剂在细胞免疫荧光中的应用
培养基的历史
如何提高细胞的转染效率
结构域(domain)
I型内含子(group I intron)
载体蛋白(carrier protein)
细胞识别(cell recognition)
MTT实验原理、步骤、结果分析方法及注意事项攻略
细胞增殖的检验方法：结晶紫法
翻译（translation）
用51Cr铬酸钠标记靶细胞
有丝分裂实验
血清在细胞培养中的基本作用
N2 supplement的配置和N2 Supplement操作手册
血清使用过程中的QA
动物细胞常用的五种培养方式
植物信号转导中的MAPK级联途径
网格蛋白(clathrin)
Proliferation Assay: [3H] Thymidine incorporation
Quantitation of 4-Hydroxynonenal Protein Adducts
复合物Ⅳ(complex Ⅳ)
流体镶嵌模型（fluid mosaic model）
关于2-D电泳的关键步骤和溶液配制
流式细胞仪技术概述
培养血管平滑肌细胞得一点体会
Human Embryonic Stem (ES) Cell Protocols――Media and Reagents
由Bcl-2家族介导的细胞凋亡图
原代细胞培养
Fibroblast Cell Systems Instructions for Use
器官培养
巨噬细胞的分离和纯化
RB基因 (Retinoblastoma gene)
Quantification of Busulfan in Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectromet
Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation
Mitochondrial DNA Oxidative Damage and Mutagenesis in Saccharomyces cerevisiae
组织和细胞的固定的两种方法
A Non-Covalent Peptide-Based Strategy for siRNA Delivery
猪白细胞介素1（IL-1）酶联免疫分析（ELISA）
Programmed Cell Death In Situ Detection Using Terminal deoxynucle
PCR-Based Site-Directed Mutagenesis
血清热灭活您是否在浪费时间？
信号趋异(divergence)
GE推出首个符合GMP制备单核细胞培养基Ficoll-Paque™ PREMIUM
酵母遗传学方法9：酵母免疫荧光
腺苷酸环化酶(adenylate cyclase, AC)
流动镶嵌模型(fluid mosaic model)
Cell-Free Assay System for Ras- and Rap1-Dependent Activation of MAP-Kinase Cascade
原核细胞
T 细胞克隆的制备
Immuno-Laser Capture Microdissection
体外哺乳类细胞（V79/HGPRT）基因突变试验－中华仓鼠肺细胞染色体突变实验
细胞介导细胞毒作用检测法1：Cr释放法
发动蛋白(dynamin)
动物细胞培养及外源基因的导入
bsa的性质与作用
体外荧光法检测核内体早期动力学
肿瘤坏死因子（tumor necrosis factor,TNF）
TISSUE FIBROBLAST CULTURES FOR CHROMOSOME ANALYSIS
Expression of Recombinant Matrix Metalloproteinases in Escherichia coli
胚胎干细胞(embryonic stem cell，ES)
Taq Polymerase (EC 2.7.7.7): With Particular Emphasis on Its Use in PCR Protocols
不同细胞siRNA转染效率检测讨论
MEM培养液出现沉淀问题解析
关于293/293T细胞基本信息
Freezing and Thawing cells
选择基因(selector genes)
DNA Cell Cycle
过氧化物酶体(peroxisome)
细胞培养――培养基和试剂
SOC、TB、YPD培养基配制
核被膜及核孔复合体
Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase
单能干细胞（unipotent stem cell）
基因性状的其它检测方法
PTEN介导的细胞生长抑制和细胞凋亡信号图
常用细胞凋亡检测方法（一）
质粒DNA的提取与纯化
Hepes缓冲液配置方法
调节型分泌途径(regulated secretory pathway)
Stabilizing Antibody Secretion of Human Epstein Barr Virus-Activated B-Lymphocytes with Hybridoma Fo
Mechanism-Based Profiling of MMPs
Liposome-Based Vaccines
初代组织块培养法
Phycoerythrin conjugation protocol
Subculture of Semi-Adherent Cell Lines
肌联蛋白(titin)
An Efficient Transfection Method for Mouse Embryonic Stem Cells
溶酶体(lysosome)
mtt实验前应明确的8个问题
Polymer Microarrays for Cellular High-Content Screening
Multilocus Sequence Typing
Purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaio
怎样使昆虫细胞适应新的培养液
自己总结的MTT方法原理
Raft culture media (aka Green’s media)
粘着斑(adhesion plaque，focal adhesion)
Lineage Tracing of Hair Follicle Stem Cells in Epidermal Whole Mounts
生物膜结构体系(biomembrane system)
蛋白质分选(protein sorting)
细胞分离中如何降低污染专家有妙招
细胞免疫荧光的操作步骤
MTT配制、原理、操作步骤、结果分析、注意事项
ORGANOTYPIC LIMB CULTURES
Phosphorylation-Dependent Regulation of SATB1, the Higher-Order Chromatin Organizer and Global Gene
293细胞的大规模培养方式
细胞建株(系)
减数分裂实验
Flow cytometric determination of leukocyte surface antigens in whol
Immunofluorescent Localization of Tubulin
细胞凋亡的过程
FACS Procedures for Apoptosis Detection
Analysis of B-Cell Signaling Using DT40 B-Cell Line
无血清培养基对体外激活的A-NK细胞的支持作用
Measurement of Spatial Proximity and Accessibility of Chromosomal Loci in Saccharomyces cerevisiae U
Photoablation of Cells Expressing β-Galactosidase
细胞介导细胞毒作用检测法2：[3H]脱氧胸苷释放法
原核细胞的种类与结构简介
Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS)
G418筛选稳定表达细胞系PROTOCOL
体外细胞的原代培养、传代培养、冻存和复苏实验方法步骤
生物安全柜安全性能解惑
The Under-Agarose Migration Assay
孔蛋白(porin)
细胞的冷冻保存
T 细胞克隆的制备
NAG检测细胞生长
胚胎干细胞培养技术大全
如何让干细胞不分化新型干细胞培养基来帮忙
孚尔根反应的方法和原理
DNA Laddering
CSF Extract Prep for Spindle Assembly
巨大染色体(giant chromosome)
Sphingosine-1-Phosphate Signaling in Neural Progenitors
细胞的冻存与复苏
哺乳动物MAPK信号通路途径图
Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display
MEM培养基使用方法
关于稳定转染的几个问题
脂质体转染的实验原理与操作步骤大全
TGFβ信号传导
Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction
人NK 细胞克隆的制备
人成纤维细胞生长因子-21（FGF-21）酶联免疫分析
人可溶性髓系细胞触发受体-1(sTREM-1)酶联免疫分析
受精作用(fertillization)
细胞质膜(plasma membrane)
通过细胞计数对疾病模型小鼠进行免疫监测
Time-Resolved NMR Spectroscopy: Ligand-Induced Refolding of Riboswitches
CD34阳性细胞计数
Methylation-Sensitive Polymerase Chain Reaction
Construction and Characterization of a Cell Line Deficient in Repair of Mitochondrial, but Not Nucle
Method: Preparation of Lymphocyte Cell Pellet for Storage
细胞培养中的污染问题小汇总
核糖体核糖核酸（ribosomal RNA，rRNA）
Physical Monitoring of HO-Induced Homologous Recombination
看家基因(house-keeping gene)
Sperm DNA Fragmentation Analysis Using the TUNEL Assay
Quantitation of Total 11-Nor-9-Carboxy-Delta 9-Tetrahydrocannabinol in Urine and Blood Using Gas Chr
Flow Cytometry Analysis of Transcription Factors in T Lymphocytes
Purification of the Poly-ADP-Ribose Polymerase-Like Thermozyme from the Archaeon Sulfolobus solfatar
精子发生（spermatogenesis）
Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or St
Quantitation of Argatroban in Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectromet
Protamine Extraction and Analysis of Human Sperm Protamine 1/Protamine 2 Ratio Using Acid Gel Electr
alamarBlue的用法
如何选择合适的细胞培养基
细胞培养污染之支原体污染检测
做MTT时药不溶于培养基怎么办
Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Sa
超声波细胞破碎仪指南
2型糖尿病大鼠动物模型
Chromosome Spreads with Centromere Staining in Mouse Oocytes
蟾蜍骨髓细胞染色体观察
核仁组织区(nucleolar organizing region, NOR)
无血清培养基的优点和种类说明
氨基酸营养缺陷型突变株的筛选
酶细胞化学技术(enzyme cytochemistry)
三种199细胞培养基成分
Somite Unit Chronometry to Analyze Teratogen Phase Specificity in the Paraxial Mesoderm
时间分辩荧光分析法
各等级BSA使用说明
Xenopus Egg Extracts: A Model System to Study Proprotein Convertases
Flow Cytometry Assay to Measure Internalization of GPCR's
去壁低渗法制备植物染色体标本
Cryopreservation of Pathogenic and Nonpathogenic Free-Living Amoebae
OPTI-MEM进行贴壁细胞培养的实验方法
Cell子刊：科学家新研发一种技术快速将iPSC转化成为老化神经细胞
Approaches to Define the Involvement of Reactive Oxygen Species and Iron in Ultraviolet-A Inducible
细胞质决定子(cytoplasmic determinants)
离子载体(ionophore)
Nucleofection
610A 采用 CytoFLEX 流式细胞仪上的紫光侧向散射光，有利于进行亚微颗粒分析和计数
蛋白酶体(proteasomes)
In Vitro Protein Synthesis
细胞培养室的设置和设备
胞外基质
电转感受态细胞的制备
CaCl2感受态细胞的制备
大鼠白细胞介素1（IL-1）酶联免疫分析
中科院青岛能源所在布朗葡萄藻培养研究中取得新进展
细胞质动力蛋白(cytoplasmic dyneins)
线粒体肿胀检测方法
Dynamic Flow Assay in a Parallel Plate Flow Chamber
Analysis of ProteinDNA Interactions During Meiosis by Quantitative Chromatin Immunoprecipitation (qC
转基因――基因标靶
吖啶橙和溴化乙啶双重染色
细胞培养常用器材与试剂配制
Cadherin-Mediated Cell-Cell Interactions
PC12 Cell Culture and Fusion
αβ微管蛋白二聚体的临界浓度
流式细胞术应用 | 漂亮完成细胞因子检测
细胞培养规程
293细胞培养特性与培养基选择
整合蛋白(integral protein)
蛋白质谱分析方法特点及其在蛋白组学研究领域中的应用
小鼠心肌细胞原代培养
Mung-Bean Nuclease 1 (EC 3.1.30.1)
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
细胞生长检测10：直接计数细胞
MTT检测细胞生长
染色体G
细胞培养和转染
细胞生长的ATP检测[荧光素酶法]
做稳定转染如何选择质粒提取试剂盒
蛋白激酶C(protein kinase C，PKC)
Sperm Selection for ICSI Using Annexin V
单位膜模型(unit membrane model)
PKC系统(protein kinase C system，PKC system)
TaqMan OpenArray High-Throughput Transcriptional Analysis of Human Embryonic and Induced Pluripotent
死亡受体的信号通路图
Laser Capture Microdissection (LCM)
细胞培养入门教程
细胞复苏方法
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) for the Genotyping of Bacterial
Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons
Immobilization of Liposomes and Proteoliposomes in Gel Beads
细胞自噬概念及研究
细胞融合
HLA-B*27基因检测在强直性脊柱炎诊断中的重要意义
死亡受体3，4，5诱导的细胞凋亡通路图
片层结构模型(Lamella structure model)
正确的细胞复苏需知事项
TNF成品生物活性的测定的操作流程
细胞凋亡的简介和常用检测方法
酵母遗传学方法10：固定化细胞的肌动蛋白染色
Differentiation of Endothelial Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Ce
Ultra-Fast and Optimized Method for the Preparation of Rodent Testicular Cells for Flow Cytometric A
细胞凋亡信号与线粒体膜
Gamma Irradiation of PMEF
生物安全柜定购指南
如何作好软琼脂实验分析
Collagen Gel Contraction Assay
Keratin 19 as a Stem Cell Marker In Vivo and In Vitro
Analysis of Mitogen-Activated Protein Kinase Activity in Yeast
凋亡小体(apoptotic body)
SD序列(SD sequence)
Exploring Functional In Vivo Consequences of the Selective Genetic Ablation of mTOR Signaling in T H
AMNIOTIC FLUID CULTURES ON COVERSLIPS
输出蛋白(exportin)
人血涂片的制作
T细胞尼龙毛柱分离法
Detecting ATM-Dependent Chromatin Modification in DNA Damage and Heat Shock Response
细胞培养基的几个问题
Arf6 and its Role in Cytoskeletal Modulation
Analysis of Amplified DNA Molecules by Capillary Electrophoresis and Laser Induced Fluorescence
Antisense rel A in Cancer
异染色质(heterochromatin)
人类若干性状的遗传特性及其调查分析
细胞外基质(extracellular matrix,ECM)
In Vivo and In Vitro Tools to Identify and Study Transcriptional Regulation of USF-1 Target Genes
Detection of a Lipid-Lysine Adduct Family with an Amide Bond as the Linkage: Novel Markers for Lipid
Counting ES cell Chromosomes
Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos
生命科学中的FISH （“钓鱼”）技术
人白细胞介素-10（IL-10）酶联免疫分析（ELISA）
Hayflick界限(Hayflick life span)
293细胞培养的经验总结
Generation of Antibodies That Are Externally Acting Isoform-Specific Inhibitors of Ion Channels
小鼠白细胞介素21（IL-21）酶联免疫分析（ELISA）
大鼠白细胞介素6（IL-6）酶联免疫分析
肿瘤细胞培养技术要点
Lentivirus的包装和感染
层粘连蛋白(laminin, LN)
Simultaneous analysis of DNA content and surface
Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free C
Isolation of a Membrane-Bound Enzyme, 5-Nucleotidase
Generation and Hepatic Differentiation of Human iPS Cells
Covalent Modification of G-Proteins by Affinity Labeling
ES and TS cell freezing/thawing
Application of a Fusiogenic Peptide GALA for Intracellular Delivery
大鼠内皮细胞的培养方案
Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold
Monitoring Oncogenic B-RAF-Induced Senescence in Melanocytes
人白细胞介素1β（IL-1β）酶联免疫分析
Thawing Cells (Schreiber protocol)
MAPK信号通路精美图片
细胞的复苏个人经验总结
EMBRYONIC STEM CELLS PROTOCOL
膜骨架(membrane skeleton)
跨膜运输(across membrane transport)
GT-AG 规则(GT-AG rule)
Hemacytometer Workbook
Cell Fusion-Induced Reprogramming
Human CD4+CD25highCD127low/neg Regulatory T Cells
细胞增殖的检验方法
微管结合蛋白(microtubule-associated proteins, MAPs)
人白细胞介素1(IL-1)酶联免疫分析（ELISA）
骨髓间质干细胞的分离和培养
Measurement of Intracellular pH
小鼠白细胞介素33（IL-33）酶联免疫分析
ERK5 MAPK信号通路图
Fingerprinting by Arbitrarily Primed PCR
推荐：细胞融合实验
细胞培养中的化学污染和生物污染
Assays to Monitor Degradation of the EGF Receptor
Microtubule (MT)/Organelle Motility Assays
抗体和样品要求上海西唐生物蛋白印迹 Western Blotting 实验服务， 021-55229872 1. 为保证质量，抗体最好为进口单克隆抗体； 2. 样本要求：尽可能新鲜； 3.
Western Blotting,实验标本处理
细胞培养板的常见问题解答
Identification of Novel Long Noncoding RNA Transcripts in Male Germ Cells
无血清技术及其培养基
Using Microchip Gel Electrophoresis to Probe DNADrug Binding Interactions
An In Vitro Assay for Cdc20-Dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast
植物多倍体细胞的诱发实验及多倍体细胞观察
Proteinase K (EC 3.4.21.14)
细胞荧光化学实验
Culture of endometrial explants
Purification and Analysis of Variant and Modified Histones Using 2D PAGE
Aptamers and Biosensors
Yeast as a Model for Ras Signalling
A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells
原肠胚(gastrula)
Retrovirus Production
细胞凋亡检测：流式细胞仪检测技术
Hemacytometer Workbook
细胞电泳
蛋白激酶A (protein kinase A，PKA)
Lyophilization of Nonviral Gene Delivery Systems
Definitions and Criteria for Stem Cells
Isolating primary B cells from human blood
线粒体膜势能检测细胞凋亡
大肠杆菌杂交
嘌呤霉素(puromycin)
小鼠的R1胚胎干细胞系培养实验步骤
兔子白细胞介素10（IL-10）酶联免疫分析
Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
Induction of Tumor Cell Apoptosis and Chemosensitization by Antisense Strategies
细胞组分和细胞器――染色体
Intracellular Delivery of Nanoparticles with CPPs
红细胞裂解液的若干配制方法
毕式酵母表达常见问题及回答
旁分泌信号(paracrine signaling)
如何区分细胞周期中的细胞与凋亡中的细胞
Isolation of Rat Liver Hepatocytes
异噬性溶酶体(heterolysosome)
KARYOTYPING ES CELLS
人白细胞介素-32（IL-32）酶联免疫分析（ELISA）
Monitoring MMP andTIMP mRNA Expression by RT-PCR
细胞凋亡有何意义？
组成细胞的基本元素和基本物质
显微镜技术――光学显微技术
血清灭活/制备/保存/使用问题的总结
Use of the Hemacytometer for the Determination of Cell Numbers
Functional Studies of Dual-Specificity Phosphatases
Detection of Mycoplasma by Culture
BrdU法细胞周期检测实验原理与操作步骤
限制点（restriction point）
Apoptosis TUNEL assay (Paraffin Sections)
研究细胞凋亡的几种光镜形态学检测方法比较
大鼠免疫球蛋白M(IgM)酶联免疫分析（ELISA）
转基因
细胞周期测定
生长因子（growth factor）
Lentivirus-Expressed siRNA Vectors Against Alzheimer Disease
果蝇遗传性状系列杂交实验
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Ligation-Mediated Polymerase C
人可溶性黏膜地址素细胞黏附分子1(sMAdCAM-1)
Multiplexing Cell Viability Assays
Origin and Progress of Nuclear Transfer in Nonmammalian Animals
Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and Cult
Determination of MiRNA Targets in Skeletal Muscle Cells
重组节(recombination nodules)
细胞凋亡是否属于分化
Stem Cell Identification by DyeCycle Violet Side Population Analysis
关于人退变椎间盘细胞原代培养的一点体会
Analysing Steroid Modulation of BKCa Channels Reconstituted into Planar Lipid Bilayers
流式细胞仪常用的几种检测方法
【视频】流式细胞术启动与清洗详解
磷酸钙转染法
卡尔文循环(Calvin cycle)
MAPK信号通路大图
造血干细胞移植及其相关危险因素
完全桥粒(desmosome)
呼吸链(respiratory chain)
生长曲线测定（计数法）
ATP合酶(ATP synthase)
Altered Expression of NFkB in Ex Vivo Differentiated Dendritic Cells from the Aged Subjects: Implica
Q带(Q-banding)
单向琼脂扩散试验（Single immunodiffusion test）
Quantification of mtDNA Mutation Heteroplasmy (ARMS qPCR)
骨骼肌细胞损伤模型的建立
293T细胞的培养实验步骤
用于免疫印迹的细胞裂解液的制备
Cell Thawing/Cell Freezing Protocol
The Ras Superfamily of Small GTPases: The Unlocked Secrets
我国“胚胎植入前胚胎遗传学诊断/筛查”达国际领先水平
幽门螺杆菌改良无血培养基的研制
染色体制片操作流程
Monitoring Blood for CD34+ Cells to Determine Timing of Hematopoietic Progenitor Cells Apheresis
Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Ext
大鼠血管内皮细胞生长因子（VEGF)酶联免疫分析
Methods to Assay Stress-Activated Protein Kinases
shRNA-Induced Interferon-Stimulated Gene Analysis
游离核糖体(free ribosomes)
关于LIPO2000转染HepG2细胞的问题
Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identi
Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization
人脐静脉内皮细胞的体外培养、鉴定与形态观察
Viral Transduction of Adipose-Derived Stem Cells
Isolation of Microtubules (Bovine Brain)
Feeder-Free Culture of hESCs
Immunofluorescent Localization of Tubulin
Ultrarapid Vitrification of Mouse Oocytes and Embryos
Using the Fluorescent Styryl Dye FM1-43 to Visualize Synaptic Vesicles Exocytosis and Endocytosis in
Liposome Formulations of Hydrophobic Drugs
Isolation of DNA Structure-Dependent Checkpoint Mutants in S. pombe
In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination
小鼠胚胎干细胞培养实验步骤
干细胞成骨诱导后的染色方法和步骤
Measuring FRET Using Time-Resolved FLIM
Multiphoton Intravital Microscopy to Study Lymphocyte Motility in Lymph Nodes
Regulation of Krpple-Like Factor 5 by Targeted Protein Degradation
间隙连接(gap junction)
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
FACS Analysis Using Peripheral Blood Cells
Staining Methods for cell death
A Tale of Two Interferon Bioassays: How Frustration with Discrepant Results from Slightly Dissimilar
羊水细胞染色体标本制备
P位点(P site)
海底地层中发现“超节能”微生物
Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay
半透性膜（semipermeable membrance）
转染真核表达载体后需要将载体线性化吗
血浆中C5a的ELISA测定
蝗虫减数分裂过程观察
Cloning Mice and ES Cells by Nuclear Transfer from Somatic Stem Cells and Fully Differentiated Cells
Polymorphisms Within Epithelial Receptors: NOD2/CARD15
p53基因
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
shRNA-Mediated Gene Knockdown in Skeletal Muscle
Human Chromosome Analysis and Sorting
Wnt信号通路图（9张）
划痕实验方法总结：如何解决划痕宽度不一致的问题
线粒体的活体染色及电镜照片观察实验
细胞凋亡及其检测技术的进展
FEEDER PREPARATION BY GAMMA IRRADIATION
Mapping the Specificity of SH3 Domains with Phage-Displayed Random-Peptide Libraries
Preparation of Collagen-Coated Gels that Maximize In Vitro Myogenesis of Stem Cells by Matching the
Crystallization of the glmS Ribozyme-Riboswitch
Cell-Based Microarrays: Overview
瘢痕组织中成纤维细胞培养
Use of Human Mesenchymal Stem Cells as Alternative Source of Smooth Muscle Cells in Vessel Engineeri
大家来谈谈CIK细胞
中心体(centrosome)
肾小球分离与移植培养
绒毛染色体制备
The Ames Test
Culture of Avian Embryos
成斑(patching)反应
乳鼠心肌的分离方法
诱变物质的微核测试
北京大学陈英玉：细胞凋亡检测方法
红细胞裂解液的配制
Adipogenic Differentiation of Adipose-Derived Stem Cells
Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining
非循环式光合磷酸化(non-cyclic photophosphorylation)
成核反应(nucleation)
前期(prophase)
核糖核酸酶P (ribonuclease P)
小鼠白细胞介素8（IL-8）酶联免疫分析（ELISA）
Protein Overexpression in Skeletal Muscle Using Plasmid-Based Gene Transfer to Elucidate Mechanisms
Functional Identification of Neural Stem Cell-Derived Oligodendrocytes
Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver as a Source of Li
核定位信号(nuclear localization signal, NLS)
Platelet Preparation
FIXATION and DNA Staining for Cell Cycle Analysis
Neural Differentiation of Human Embryonic Stem Cells
核输出信号(nuclear expotr-signal,NES)
Cell-Adhesion Assays
7- 3D Assay in Matrigel : S1, T4-2, T4-2R
Isoelectric Focusing and Immunofixation of Monoclonal Antibodies
大鼠肾素（Renin）酶联免疫分析（ELISA）
Two-Dimensional Blue Native/Blue Native Polyacrylamide Gel Electrophoresis for the Characterization
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
染色体Ｃ带技术
猴白细胞介素2（IL-2）酶联免疫分析（ELISA）
骨髓间充质干细胞分离培养经验
Method: Removal of Yeast Contamination from Lymphoblast Cultures
酵母遗传学方法12：酵母菌落PCR方法
电子显微镜生物标本的制备及观察
MINICHROMOSOME MICROTUBULE BINDING ASSAY
Preparation and Characterization of Posttranslationally Modified Tubulins From Artemia franciscana
大鼠平滑肌细胞的另一种培养方法
T淋巴细胞的分离技术-尼龙毛法
肝枯否细胞分离的个人经验
流式细胞术测效靶细胞杀伤实验
磁性转染：一种新型的基因转染技术
Culturing Chick Embryonic Myoblasts/Myotubes and Fibroblasts
抗纤维化药物临床实验的新趋势：策略与方法
Langendorff离体心脏缺血再灌注方案
Pronase (EC 3.4.24.4)
NK-cell cytotoxicity assay
单细胞CMC试验
枯草杆菌细胞固定化
线粒体膜与促细胞凋亡蛋白
酵母遗传学方法4：质粒DNA的羟基突变
细胞爬片中重点注意事项
Derivation and Characterization of Hematopoietic Cells From Human Embryonic Stem Cells
小鼠白细胞介素-12（IL-12）酶联免疫分析（ELISA）
Target Validation of Plant microRNAs
细胞周期(cell cycles)
Inducible raptor and rictor Knockout Mouse Embryonic Fibroblasts
Rapamycin as Immunosuppressant in Murine Transplantation Model
固定细胞的PI单染色法
Amplified Fragment Length Polymorphism Analysis
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
染色质(chromatin)
Colorimetric Screen for Aliphatic Hydroxylation by Cytochrome P450 Using p-Nitrophenyl-Substituted A
Cambrex推出前脂肪细胞生长培养基试剂盒PGM-2
流式细胞凋亡检测--疑难解答
大豆β-伴球蛋白(β-conglycinin)酶联免疫分析（ELISA）
人白细胞介素-8（IL-8）酶联免疫分析（ELISA）
Capillary Electrophoresis of Neutral Carbohydrates: Mono-, Oligosaccharides, Glycosides
反义RNA(antisense RNA)
Analyzing Mammalian Female Meiosis
细胞计数和存活测试
Human Alkaline Phosphatase staining of transgenic embryo/tissue
Coimmunoprecipitation on Drosophila Cells in Culture
无菌操作注意事项
Cell Proliferation Assay
Cdc2蛋白(Cdc 2 protein)
糖原的显示—PAS反应（periodic acid schiff’s reaction）
Evaluation of mTOR-Regulated mRNA Translation
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion
XTT
猴白细胞介素17（IL-17）酶联免疫分析（ELISA）
Chemotaxis Assay
Studying Meiosis-Specific Cohesins in Mouse Embryonic Oocytes
小鼠基质细胞衍生因子1(SDF-1)酶联免疫分析
细胞培养反应器的几种操作模式
人类淋巴母细胞建株要点
由IGF-1R导致BAD磷酸化的细胞凋亡信号通路
磷酸钙法转染HEK293T细胞实验方法
MTT测定CMC的操作步骤
核仁组织者区的AgNO3染色
Rapid Sequencing of cDNA Clones Direct Sequencing Using Sequential Linear/Asymmetric PCR
Culture of BEND Cells (Bovine Endometrial Cells)
Cajal Body Isolation Protocol
Labeling Tubulin and Quantifying Labeling Stoichiometry
利用哺乳动物细胞表达外源蛋白的研究进展
交联蛋白(cross-linking protein)
细胞介导的细胞毒作用（CMC）检测法
流式细胞术应用 | PBMC T 细胞受体表征检测
流式细胞仪操作规程
细胞遗传学――比较基因组杂交(CGH)
A Method to Isolate and Purify Human Bone Marrow Stromal Stem Cells
上皮细胞培养
Homology Modeling and Simulations of Nuclease Structures
定位候选克隆
骨髓细胞染色体的畸变试验
Cryopreservation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
MTT溶液的配制方法
老年痴呆的动物模型
FACS Enrichment of Human Keratinocyte Stem Cells
Investigating 5-Hydroxymethylcytosine (5hmC): The State of the Art
Effects of Collection, Processing, and Storage on RNA Detection and Quantification
随体(satellite)
16-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electropho
人胱抑素C（CysC）酶联免疫分析（ELISA）
In Vitro Chemiluminescence Assay to Measure Excision Repair in Cell Extracts
Culture Systems: Embryo Density
Recommended working medium, trypsin volume and cell inoculation
DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOS
Mapping Origins of DNA Replication in Eukaryotes
HOW TO USE THE COULTER COUNTER TO COUNT CELLS
ES and TS cell freezing/thawing
血吸虫型肝硬化模型制备
In Vitro Fertilization and Embryo Transfer in Felids
神经干细胞(neural stem cell，NSCs)
大规模培养技术应用简介
Retrovirus-Mediated RNA Interference
Protocols for Investigating microRNA Functions in Human Neural Progenitor Cells
避免牛血清沉淀的小技巧
The Scientist：活细胞病毒观察指南
圆球体
Propagation and Differentiation of Human Whartons Jelly Stem Cells on Three-Dimensional Nanofibrous
Mitochondrial DNA Isolation from Somatic Embryogenic Cell Cultures of Larix (Excerpt from Thesis).
Oligonucleotide-Targeted RNase H Protection Analysis of RNA-Protein Complexes
Detection of Carbonylated Proteins in 2-D SDS Page Separations
Biochemical and Pharmacological Inhibition of mTOR by Rapamycin and an ATP-Competitive mTOR Inhibito
衔接蛋白(adaptin, AP)
Chemiluminescent Detection of Senescence-Associated Galactosidase
Routine Splitting and freezing of cells
Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo
单一序列(unique sequence)
小鼠骨保护素（OPG）酶联免疫分析（ELISA）
Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells
Methods for Detecting Mutations in the Human JAK2 Gene
Cloning by Limiting Dilution of Hybridoma
胞吐作用(exocytosis)
Xeno-Free Culture of Human Pluripotent Stem Cells
锌指结构基元(zinc finger motif)
Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins
In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C
人白细胞介素33(IL-33)酶联免疫分析（ELISA）
Engineering of a 3D Nanostructured Scaffold Made of Functionalized Self-Assembling Peptides and Enca
Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR
细胞培养常见问题解析
Freezing cells in liquid nitrogen
Analysis of Pyroptosis in Bacterial Infection
DNA导入哺乳动物细胞常见的方法
Chlamydomonas reinhardtii: The Model of Choice to Study Mitochondria From Unicellular Photosynthetic
Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Ce
内吞（endocytosis）
传代培养细胞染色体显示法
信号分子(signaling molecules)
Monitoring Neuropeptides In Vivo via Microdialysis and Mass Spectrometry
RNA Interference Using a Plasmid Construct Expressing Short-Hairpin RNA
Micro-CGH: Microdissection-Based Comparative Genomic Hybridization
细胞培养中的无菌操作技术
Components and Supplementation of Various Culture Media
大鼠白细胞介素4（IL-4）酶联免疫分析
Integrin-Targeted Nanoparticles for siRNA Delivery
中心体循环(centrosome cycle)
PI3K/Akt通路的负性调节因子-PTEN
Identification of Epithelial Stem Cells In Vivo and In Vitro Using Keratin 19 and BrdU
Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography
复合物I( complex I)
Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemical
Reverse Transcriptase (EC 2.7.7.49): The Use of Cloned Maloney Murine Leukemia Virus Reverse Transcr
细胞系与细胞株的区别
Tissue-Specific Ablation of Tsc1 in Pancreatic Beta-Cells
Secretion of scFv Antibody Fragments
人类外周血染色体制备
人可溶性细胞间粘附分子2(sICAM-2)酶联免疫分析（ELISA）
Osteoclast Formation in Mouse Co-cultures
Ficoll密度梯度离心法
起始转移信号(start-transfer signal)
银染核仁形成区与近端着丝粒染色体随体联合
胚胎诱导(embryonic induction)
细胞松弛素B(cytochalasins B)
Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31)
Staining Procedure for Flow Cytometric Detection of Human Cyclins
如何做好磷酸钙-DNA共沉淀法实验？
别再让你培养的细胞睡在硬板床上了！！
神经细胞培养的注意事项大总结
细胞介导细胞毒作用检测法7：单细胞CMC试验
细胞冷冻保存
自分泌信号(autocrine signaling)
转染细胞后如何配制筛选培养液
三种DMEM细胞培养基成分
DNA的细胞化学:孚尔根反应（Feulgen Reaction）
Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNA Interference
Method for Conducting Microarray Study of Oxidative Stress Induced Gene Expression
Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene Repression
Isolation of Extracellular Membranous Vesicles for Proteomic Analysis
Cytofluorometric Assessment of Cell Cycle Progression
动物细胞大规模培养工艺进展与相关专利介绍
Transwell侵袭实验总结－概念
小鼠白细胞介素-12抗体（IL-12 Ab）酶联免疫分析
信号识别颗粒(signal recognition partical, SRP)
转染与转化的联系与区别
293细胞特性概述
Yeast Two-Hybrid Screening for Proteins Interacting With the Anti-Apoptotic Protein A20
细胞HE染色法实验
Ａβ损伤模型
Structural Characterization of the Carbohydrate Moieties of Glycoproteins by High-Resolution 1H-NMR
人神经元培养protocol
胰岛素受体底物(insulin receptor substrate，IRSs)
Colorimetric Dehydrogenase Screen Based on NAD(P)H Generation
染色体的标本制作及其组型实验
犬白细胞介素 1(IL-1)酶联免疫分析（ELISA）
细胞介导的细胞毒作用（CMC）检测法：细胞的制备
流式细胞仪PI染色死细胞方法
24孔板里293细胞转染后面的孔里细胞死亡原因
Analysis of Endothelial Barrier Function In Vitro
高尔基复合体(Golgi complex)
细胞生理活动的实验观察
大分子导入细胞技术
窜扰(crosstalk)
MTT实验两年心得
Direct/Indirect Staining Protocol
MAPK/ERK信号通路图及简介
SPRi实时检测淋巴细胞和抗体芯片的相互作用
各种感受态细胞的区别用途特征
大肠杆菌细胞超声波破碎
Immunoelectrophoretic Techniques: 2-D Immunoelectrophoresis and Rocket Immunoelectrophoresis
冻存管撒进液氮中了怎样取出来
Senescent-associated b-Gal Assay
R&D Systems 验证神经祖细胞多能性的试剂盒
输入蛋白(importin)
细胞形态观察及大小测量
Gomori染色(骨型碱性磷酸酶染色)
Foxp3: Treg的主要调节因子
基因转染技术应注意事项
如何看流式细胞术结果中的图
铁硫蛋白(iron-sulfur proteins, Fe/S protein)
染色单体
ELISA实验中的封闭液等常用试剂的配制
人白细胞介素-4（IL-4）酶联免疫分析（ELISA）
常用细胞凋亡检测方法（四）
High-Throughput RNAi Screen in Drosophila
MTT法和CCK－8测定细胞毒性和增值的优劣比较
Rodent Osteoclast Cultures
MTT实验中关于细胞接种的方法讨论
CCK-8较MTT法―测定细胞增殖/毒性实验
pIRES2－eGFP转染细胞经验交流
Microfluidic Image Cytometry
养293、293T系列细胞的3点体会
细胞膜电位的测定方法
细胞显微摄影
Restriction Endonuclease Digestion and Agarose Gel Electrophoresis of DNA
我的干细胞DAPI染色方法
植物多倍体的诱导与鉴定
3D细胞悬滴培养板，让您的细胞有回家的感觉
进行真核转染的一般程序
稳定表达细胞株的建立
结构性异染色质(constitutive heterochromatin)
细胞的生长参数
关于HEK293细胞转染的问题
Human Embryonic Stem Cells Derived in Xeno-Free Conditions
DNA重排(DNA rearrangement)
MMI新推出单细胞获取完美方案
检测DNA分析细胞周期
磷酸化运输(phosphorylating transport)
Multiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte Research
Introduction to Fluorescence Microscopy
JM109，DH5a，BL21这些感受态有何区别
JM109，DH5a，BL21感受态有何区别
MAPK信号通路研究工具
细胞生物学实验室操作规范
293转染没有表达荧光经验交流
钙调蛋白(calmodulin)
细胞培养技术扫盲
Annexin V/PI双染色法
小鼠白细胞介素1（IL-1）酶联免疫分析（ELISA）
Regulation of Chromatin Structure by PARP-1
DH5a感受态细胞的制备
Measurement of Cell Adhesion Under Static Conditions
【值得推荐】一种简单实用的细胞冻存方法
稳定转染：关于G 418各种信息与心得
NK-cell cytotoxicity assay
细胞培养用液
几种细胞转染技术原理和特点分析
40种常用细胞培养基配方
单层贴壁细胞-细胞毒性试验-四唑盐（MTT）比色法-实战篇
Image Analysis and Quantitative Morphology
肾小管细胞氧化性损伤模型
In Vitro T Cell Activation
细胞传代实验步骤
细胞消化的个人经验小结
低密度的脂蛋白(low-density lipoprotein, LDL)
光面内质网(smooth endoplasmic reticulum, SER)
大鼠心肌肌钙蛋白Ⅰ(CTn-Ⅰ)酶联免疫分析(ELISA)
荧光检测CMC
Transwell侵袭实验总结－Transwell的其他应用的实验步骤
几种休克动物模型得建立
维生素B2和维生素C的含量测定
Cell Staining for Senescence-Associated beta-Galactosidase (SA-beta-Gal) Activity
Identifying Different Types of Chromatin Using Giemsa Staining
流式细胞仪做血细胞
TE或水溶的质粒对转染效率的影响
收到细胞的处理方式
细胞培养技术在细胞学、胚胎学和药学方面应用
结晶紫染色测定细胞数
LDH（乳酸脱氢酶）法细胞毒性检测
细胞凋亡检测操作步骤之ELISA法
PARP1 Genomics: Chromatin Immunoprecipitation Approach Using Anti-PARP1 Antibody (ChIP and ChIP-seq)
腺病毒载体的优点
血清正确的使用方法
第一代腺病毒载体重组的常见方法
Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy
细胞活化与细胞复苏
Measurement of Oxidized/Reduced Glutathione Ratio
Experiments on Enzyme Induction in the Unicellular Green Alga Chlorella fusca
细胞组分和细胞器――细胞骨架
内质网（endoplasmic reticulum，ER）
酸性磷酸酶的显示方法
细胞培养实验的经验总结
脂锚定蛋白(lipid-anchored)
原位末端标记技术检测细胞凋亡
Spectral Karyotyping and Fluorescence In Situ Hybridization of Murine Cells
Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
化学渗透假说(chemiosmotic coupling hypothesis)
Screening of Antigen-Specific Antibody-Secreting Cells
293细胞图片
细胞凋亡检测-流式细胞仪检测技术
细胞调亡的研究方法：用荧光激活的细胞分类仪
大鼠可溶性细胞间粘附分子1(sICAM-1)酶联免疫分析（ELISA）
流式细胞分析分选术
几种检测凋亡的方法
酵母遗传学方法5：酵母β－半乳糖苷酶的测定
各类上皮细胞培养
Genetic Dissection of PARylation in the Filamentous Fungus Neurospora crassa
细胞介导细胞毒作用检测法
流式细胞术简介
肺泡2型细胞的分离培养
Protein Antigen Expression in Escherichia coli for Antibody Production
细胞培养的血清血清解冻灭活等问题
帕金森体外模型
细胞凋亡检测的各个阶段的对应方案
Hela细胞传代培养的操作步骤
电化学梯度(electrochemical gradient)
内质网滞留信号(ER retention signal)
TOP10 chemically competent cells
Extraction of Chromatin
人白细胞介素5（IL-5）酶联免疫分析（ELISA）
Chromatin Immunoprecipitation Assays for Myc and N-Myc
Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dyna
Fluorescence Recovery After Photobleaching
Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the Raclure Method
Simultaneous analysis of DNA content and surface immunophenotyp
UVB致皮肤角质形成细胞的损伤模型的制作方法
兔成骨细胞与骨髓基质干细胞混合培养的实验研究
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
Analysis of Signal Transduction: Formation of cAMP, cGMP, and Ins(1,4,5)P3 In Vivo and In Vitro
Cytoplasmic mRNA Polyadenylation and Translation Assays
重链结合蛋白(heavy-chain binding protein, Bip)
Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells
Immunohistochemical Staining of Cyclooxygenases with Monoclonal Antibodies
Preparation of cytospin from single cell suspension.
细胞凋亡实验
Noble Agar Assay
骨髓间充质干细胞的主要表面标志
Staining Methods for cell
实验：用淋巴细胞分离液分离单个核细胞
细胞自发转化spontenous transformation
支原体(mycoplasma)
Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfe
Modulation of the Host Skeletal Muscle Niche for Donor Satellite Cell Grafting
Measuring the Absolute Abundance of the Smad Transcription Factors Using Quantitative Immunoblotting
Tissue Culture Media
液态镶嵌模型
Preparation of Segmented and Polarity Marked Microtubules
MAPK signaling pathway - Homo sapiens (human)
细胞坏死(necrosis)
Basic Method for Direct Immunofluorescence Labeling
原代软骨细胞分离培养的总结
肿瘤细胞的培养
细胞培养基中常用氨基酸及其作用
溶酶体（lysosome）
人粒细胞-巨噬细胞集落刺激因子（GM-CSF）酶联免疫分析
血小板活化检测方法
电子显微镜的使用与细胞超微结构观察
lipofectamine 2000脂质体转染293ft细胞经验
果蝇的外部形态观察及饲养技术
转染实验需知
最全面的MTT相关技巧大汇总
细胞培养常见26个问题
单克隆抗体技术(monoclonal antibody technique)
如何选用特殊细胞系培养基？
细胞生长检测4：MTS检测法
Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination
Cyclic AMP- Dependent Protein Kinase Activity Ratio Assay
Coimmunoprecipitation Assay
Sperm Morphology Assessment Using Strict (Tygerberg) Criteria
The Perinucleolar Compartment (PNC): Detection by Immunohistochemistry
细胞生物基本方法：肿瘤细胞培养
大鼠III型胶原蛋白（COL-III）酶联免疫分析（ELISA）
同源异型突变(homeotic mutation)
植物原生质体的制备
DEAE-葡聚糖介导的转染步骤
TISSUE CULTURE ON COVERSLIPS
胰岛素受体(insulin receptor)
无血清培养基
Nature子刊上的akt pathway的信号通路图
人成纤维细胞生长因子23（FGF-23）酶联免疫分析
Freezing and Thawing of MEFs
Protocol for Aortic Ring Assay
吞饮作用(pinocytosis)
核糖体（ribosome）
酵母遗传学方法6：羧肽酶Y的平板鉴定
流式细胞仪之细胞凋亡检测的PI双染色法
Use of EIA to Measure MMPs and TIMPs
Apoptosis Induction
激素(hormone)
Meiotic Cytogenetics in Coprinus cinereus
Scaffolds for Tissue Engineering and 3D Cell Culture
PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION (2 METHODS)
AKT信号通路概述
新的高内涵成像系统acumen® hci隆重上市
无水氯化镁黑色液体的特性及应用解析
细胞核(nucleus)
Studying Amino Acid Transport Using Liposomes
从G蛋白偶联受体到MAPK的通路图
Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes
Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Prot
The Production of Monoclonal Antibodies to Membrane Proteins
Use of Liposomes to Study Cellular Osmosensors
质体转染293细胞后细胞漂浮解答
姐妹染色单体分化染色方法
人中性粒细胞弹性蛋白酶（NE）酶联免疫分析（ELISA）
细胞凋亡形态学检测与观察
Preparing chemically competent cells
Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Location
Identification of Target Genes of a Yeast Transcriptional Repressor
Analysis of Skeletal Muscle Development in Drosophila
Studies of Adult Neural Stem Cell Migration
Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays
胸腹水染色体标本制备
Approaches to Cloning of Pain-Related Ion Channel Genes
ES Cell-Mediated Conditional Transgenesis
细胞培养的微生物污染排除
Imaging Lipid Bodies Within Leukocytes with Different Light Microscopy Techniques
小白鼠染色体的制备和观察
交换
Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors
RNA Extraction From Drosophila Heads
Linearization and Purification of the Shuttle Plasmid
Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extract in Semi-Intact MDCK Cells
内膜系统（endomembrane system）
Detection of Tight Junction Barrier Function In Vivo by Biotin
Single Cell Analysis of Lipid Rafts
Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes
Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells
Isolation and Culture of Primary Human Hepatocytes
Expression of Recombinant Membrane-Type MMPs
Computational Prediction of RNA Structural Motifs Involved in Post-Transcriptional Regulatory Proces
Identification of Urine Organic Acids for the Detection of Inborn Errors of Metabolism Using Urease
Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta
Purification Through Affinity Chromatography and Microsequencing
Local Application of Antisense for Prevention of Restenosis
小泡运输(transport by vesicles)
前中期(prometaphase)
The Measurement of Nitric Oxide and Its Metabolites in Biological Samples by Ozone-Based Chemilumine
Protein Degradation Via the Proteosome
Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans
Effects of Temperature Generated from the Holmium: YAG Laser on Human Osteoblasts in Monolayer Tissu
Beta-gal staining of eukaryotic cells in vitro
Assays of MSCs with Microarrays
Oligonucleotide FISH Probes
Detecting Tagged Hedgehog with Intracellular and Extracellular Immunocytochemistry for Functional An
动物细胞大规模微载体培养技术
Transforming chemically competent cells
The Analysis of tRNA Import Into Mammalian Mitochondria
Quantitation of Phencyclidine (PCP) in Urine and Blood Using Gas Chromatography-Mass Spectrometry (G
Comparison of lysis methods
Amniote Phylogenomics: Testing Evolutionary Hypotheses with BAC Library Scanning and Targeted Clone
Application of Adenoviral Vectors: Analysis of Eye Development
Interspecific Chimeras: Transplantation of Neural Crest Between Mouse and Chick Embryos
细胞生物基本方法：冻存
The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies
Pharmacogenomics of Membrane Transporters: An Overview
Ⅱ型糖原贮积症(glycogen storage disease type Ⅱ)
动物细胞培养实验室必备仪器
Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos
In Vitro Generation of Megakaryocytes and Platelets from Human Embryonic Stem Cells and Induced Plur
Notochord Grafts
An Assay to Monitor the Membrane Integration of Single-Span Proteins
Spectral Bio-Imaging and Confocal Imaging of the Intracellular Distribution of Lipoplexes
Ex Vivo Megakaryocyte Expansion and Platelet Production from Human Cord Blood Stem Cells
细胞生理学(cytophysiology)
基因导入细胞的常用方法
Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence
Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells
Quantification of Cell Fusion by Flow Cytometry
Analysis of Unstable Triplet Repeats Using Small-Pool Polymerase Chain Reaction
Proteomics Studies After Hematopoietic Stem Cell Transplantation
A Functional Genomics Approach to Identify and Characterize Oxidation Resistance Genes
Progress Toward a Synthetic Virus: A Multicomponent System for Liver-Directed DNA Delivery
Hematopoietic Colony-Forming Cell Assays
In Vitro Two-Dimensional Endothelial Differentiation of Human Embryonic Stem Cells
Subculture of Suspension Cell Lines
Triplet Repeats and DNA Repair: Germ Cell and Somatic Cell Instability in Transgenic Mice
Characterization of Megakaryocyte Development in the Native Bone Marrow Environment
Activity-Based Protein Profiling of Protein Tyrosine Phosphatases
Mismatch Repair Assay
Color-Changing Karyotyping (CCK), an M-FISH/SKY Alternative
Cell Death in Myoblasts and Muscles
Cryopreservation of Seeds
Hematopoietic Development of Human Embryonic Stem Cells in Culture
纤毛动力蛋白(ciliary dynein)
副节(parasegment)
Electroporation of Drosophila Embryos
Directed Evolution of the Substrate Specificities of a Site-Specific Recombinase and an Aminoacyl-tR
人白细胞介素32γ(IL-32γ)酶联免疫分析（ELISA）
Comprehensive Hematopoietic Stem Cell Isolation Methods
生物显微摄影(Microphotography)
细胞分化的调节
正常细胞与肿瘤细胞常规核型的标本制备
Measurement of Neutrophil Elastase, Proteinase 3, and Cathepsin G Activities using Intramolecularly
Investigating Microtubule Dynamic Instability Using Microtubule-Targeting Agents
Induced Pluripotent Stem Cells (iPSC) from Cord Blood CD133+ Cells Using Oct4 and Sox2
Development of Immortalized Hepatocyte-Like Cells from hMSCs
Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants
Primary Culture and Differentiation of Human Adipocyte Precursor Cells
DNA Damage Quantitation by Alkaline Gel Electrophoresis
Patch-Clamping Drosophila Sensory Neurons
In Vivo Quantitative Proteomics: The SILAC Mouse
A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors
Nonclinical and Clinical Experiences with CPP-Based Self-Assembling Peptide Systems in Topical Drug
Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice
Assisted Reproductive Technology in Nonhuman Primates
Imaging of Intracellular Hydrogen Peroxide Production with Hyper Upon Stimulation of Hela Cells with
Methods for the Purification of HQ-Tagged Proteins
Culturing HEK 293 Cells
细胞生物基本方法：复苏
Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions
High Resolution Fluorescent In Situ Hybridization in Drosophila
An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells
Cultures of Human Adipose Precursor Cells
Primary Mouse Keratinocyte Culture
Gene Expression in Mouse Oocytes by RNA-Seq
Localization of mRNA in Vertebrate Axonal Compartments by In Situ Hybridization
The In Vivo Evaluation of Active-Site TOR Inhibitors in Models of BCR-ABL+ Leukemia
Analysis of Small Hsp Phosphorylation
Electrical Impedance Spectroscopy for Rapid and Noninvasive Analysis of Skin Electroporation
Preparation of Segmented and Polarity Marked Microtubules
Chemotaxis in Neutrophil-Like HL-60 Cells
Immunolocalization in the Epidermis
In Vivo Analysis of Ribonucleoprotein Complexes Using Nucleotide Analog Interference Mapping
Noncovalent Protein Interaction with Poly(ADP-Ribose)
Expression Profiling Using Quantitative Hybridization on Macroarrays
Isolation of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammali
Methylated Polyamines as Research Tools
Subcellular Fractionation of Adipocytes and 3T3-L1 Cells
Manipulating Claudin Expression in Avian Embryos
Deriving Metabolically Active Hepatic Endoderm from Pluripotent Stem Cells
Antisense Tumor Therapy: Activated C-Ha-ras Oncogene in the Mouse
Immunocytochemical Analysis of Human Pluripotent Stem Cells
Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells
Antisense Strategies for Redirection of Drug Metabolism: Using Paclitaxel as a Model
滤泡细胞(follicle cell)
大鼠血小板活化因子受体（PAFR）酶联免疫分析（ELISA）
Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts
Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells
Instrumentation and Electrodes for In Vivo Electroporation
Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes
Recording Single-Channel Currents Using Smart Patch-Clamp Technique
Identifying the Spatial Distribution of Vitamin E, Pulmonary Surfactant and Membrane Lipids in Cells
膜(membrane)
Retroviral Transduction of Hematopoietic Progenitors Derived From Human Embryonic Stem Cells
Multifluorescence Labeling and Colocalization Analyses
Site-Directed Mutagenesis Using Positive Antibiotic Selection
The Coculture Method to Examine Interactions Between Claudin Isoforms in Tight Junction-Free HEK293
Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spi
Monitoring Patterns of Gene Expression in Dictyostelium by -galacotsidase Staining
Skin Morphogenesis: Embryonic Chicken Skin Explant Cultures
Labeling and Purification of RNA Synthesized by In Vitro Transcription
Neural Differentiation of Embryonic Stem Cells
A rapid and highly sensitive method for somatic mutation profiling using the Sequenom MassARRAY® Sys
Gene Transfer into Cystic Fibrosis Airway Epithelial Cells
Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies
Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival
Protoplast Transformation of Filamentous Fungi
Purification of Peripheral Blood Natural Killer Cells
Detection of Microsatellite Instability in Cancers by Means of Nongel-Sieving Capillary Electrophore
ES Cell Culture and Manipulation
Peptide Nanofiber Scaffolds for Multipotent Stromal Cell Culturing
Use of Polyamine Metabolites as Markers for Stroke and Renal Failure
Plastic dishes for growing cells
Simultaneous Fluorescence Immunostaining and FISH
The Basis of Electrochemotherapy
Isolation and Functional Analysis of Mitochondria From the Nematode Caenorhabditis elegans
ATP Production in Isolated Mitochondria of Procyclic Trypanosoma brucei
Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics)
Liposomes for Specific Depletion of Macrophages from Organs and Tissues
Isolation of a Sequence-Specific RNA Binding Protein, Polypyrimidine Tract Binding Protein, Using RN
Using Schizosaccharomyces pombe Meiosis to Analyze DNA Recombination Intermediates
How to Assess and Study Cell Death in Dictyostelium discoideum
Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic
Ultrastructural Imaging of Cell Fusion in Caenorhabditis elegans
Proteomics Method for Identification of Pseudopodium Phosphotyrosine Proteins
MITOMYCIN C TREATMENT OF PMEFs
Quantitation of Orotic Acid in Urine Using Isotope Dilution-Selected Ion Gas Chromatography-Mass Spe
Identification of Specific Protein-RNA Target Sites Using Libraries of Natural Sequences
Characterization of Human Adipose-Derived Stem Cells Using Flow Cytometry
Microalgal Immobilization Methods
In Vitro Selection of Aptamers from RNA Libraries
Antioxidant QSAR Modeling as Exemplified on Polyphenols
Mesenchymal Cell Fusion in the Sea Urchin Embryo
Photoorientation in Photosynthetic Flagellates
Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistoche
Production of Chimeric Chickens
Electrochemotherapy: Animal Model Work Review
Micro-injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes
Panel Development for Multicolor Flow-Cytometry Testing of Proliferation and Immunophenotype in hMSC
Culture Technique of Human Pituitary Adenoma Cells
Detection of Expression Patterns in Hydra Pattern Formation
Culture Systems: Physiological and Environmental Factors That Can Affect the Outcome of Human ART
Embryo Culture and Epigenetics
Gene Expression in Polytene Nuclei
SopE-Mediated Recruitment of Host Rab5 on Phagosomes Inhibits Salmonella Transport to Lysosomes
Mapping Cis- and Trans- Chromatin Interaction Networks Using Chromosome Conformation Capture (3C)
Combined Peptidomics and Genomics Approach to the Isolation of Amphibian Antimicrobial Peptides
Using Drosophila S2 Cells to Measure S phase-Coupled Protein Destruction via Flow Cytometry
PTDDRBD siRNA Delivery
细胞培养中的黑胶虫问题
Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins
Isolation, Purification, and Growth of Human Skeletal Muscle Cells
Live Imaging Mouse Embryonic Development: Seeing Is Believing and Revealing
Promoter Analysis of TGF-Responsive Genes by Transient Transfection and Deletional/Mutational Analys
Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for
Induction of Ca2+ Flux by Adhesion Molecules in Lymphocytes
Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxyg
Determination of the Clinical Significance of an Unclassified Variant
A Live Cell Micro-imaging Technique to Examine Platelet Calcium Signaling Dynamics Under Blood Flow
DNA laddering assay for treated cells
Detecting Recruitment of DNA Damage Response Factors Through the eChIP Approach
Transfection and Promoter Analysis in Embryonic Stem Cells
Methods to Analyze Transglutamination of Proteins Involved in Apoptosis
Scanning for Transcription Factor Binding by a Variant EMSA
A Rapid and Selective Mass Spectrometric Method for the Identification of Nitrated Proteins
Analysis of Tissue Interactions in Ectodermal Organ Culture
Use of Repetitive Element Palindromic PCR (rep-PCR) for the Epidemiologic Discrimination of Foodborn
Antioxidant Activity of Biotransformed Sex Hormones Facilitated by Bacillus stearothermophilus
The Mycobacterium tuberculosis Phagosome
活体成像中荧光色素标记细胞的方法举例
Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture
Bioluminescence Imaging of Bone Metastasis in Rodents
Post-transplant Monitoring of Chimerism by Lineage-Specific Analysis
Analyzing Cell Death Events in Cultured Leukocytes
一氧化氮、总抗氧化能力对大鼠隐睾生殖细胞凋亡的影响
293细胞的大规模培养
Detection and Analysis of (O-linked -N-Acetylglucosamine)-Modified Proteins
Application of Cell Traction Force Microscopy for Cell Biology Research
The Dansyl-Edman Method for Peptide Sequencing
Cotransfection Assays and Steroid Receptor Biology
ECM Macromolecules: Rotary Shadowing and Scanning Transmission Electron Microscopy
Culture Systems: Embryo Culture and Monozygotic Twinning
Isolation of Membrane Rafts and Signaling Complexes
Alkaline Phosphatase (EC 3.1.3.1)
内含子(intron)
Enhanced Cellular Delivery of Cell-Penetrating PeptidePeptide Nucleic Acid Conjugates by Photochemic
A Dot Blot Immunoassay for UV Photoproducts
Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro
In Vitro Histone Demethylase Assays
Methods for Functional Analysis of Stem Cells
Mutation Detection in the TGF Receptors and SMAD Genes: RT-PCR and Sequencing
Human Pluripotent Stem Cells: The Development of High-Content Screening Strategies
Environment-Responsive Multifunctional Liposomes
Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs
Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin
Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs
Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin
Detection and Characterization of Receptor Interactions with PDZ Domains
Investigating Myc-Dependent Translational Regulation in Normal and Cancer Cells
Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
Chromatophores as Tools for the Study of Organelle Transport
Reliable Method for Detection of Programmed Cell Death in Yeast
Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos
Hematopoietic Stem Cell Mobilization: A Clinical Protocol
Fibrin Gel Model for Assessment of Cellular Contractility
Discrete Dynamic Modeling of Signal Transduction Networks
Quantification of Antidepressants Using Gas Chromatography-Mass Spectrometry
Isolation, Culture, and Osteogenic/Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal S
RIP: An mRNA Localization Technique
Crystallization of Membrane Proteins
Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteins
Peptidomics Analysis of Lymphoblastoid Cell Lines
Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells
电位-门控通道(voltage-gated channels)
“夸娥”因子的作用介绍
Inhibition of PI3K-Akt-mTOR Signaling in Glioblastoma by mTORC1/2 Inhibitors
Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand
In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Character
Revealing the Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling Microarray
Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern
Quality Control Considerations
Purification of the Ndc80 Kinetochore Subcomplex From Xenopus Eggs
In Vitro Genetic Analysis of RNA-Binding Proteins Using Phage Display
Human Embryonic Stem (ES) Cell Protocols――General notes on ES cell culture
Endothelial wound healing (cell migration) assay
Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues
Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent
Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications
Aminopeptidases: Aminopeptidase M (EC 3.4.11.2), Pyroglutamate Aminopeptidase (EC 3.4.19.3), and Pro
Chicken Embryonic Stem Cells: Establishment and Characterization
Current Trends in Liposome Research
Inflammation and Wound Healing in Drosophila
Utility of Animal Models for Human Embryo Culture Development: Rodents
Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview
Analyzing the Developmental Expression of Sigma Factors with S1-Nuclease Mapping
Pooling Data When Analyzing Biomarkers Subject to a Limit of Detection
Developmental Control of Growth and Cell Cycle Progression in Drosophila
细肌丝(thin filament)
Isolating RNA with the Cationic Surfactant, Catrimox-14
Use of Necrotic Markers in the Drosophila Ovary
细胞实验原创经验
Screening for Mutations in Cartilage ECM Genes
Pronuclear Microinjection of Mouse Zygotes
分子内伴侣(intramolecular chaperones)
大鼠内皮型NO合酶(eNOS）酶联免疫分析（ELISA）
Biochemical Analysis of Claudin-Binding Compatibility
Cryopreservation of Human Gametes
Ovariectomy/Orchidectomy in Rodents
Biologically Based Risk Assessment Models for Developmental Toxicity
Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids
Polo-Like Kinase-1: Activity Measurement and RNAi-Mediated Knockdown
Gene Expression Analysis by In Situ Hybridization: Radioactive Probes
Cryofixation Methods for Ultrastructural Studies of Dictyostelium discoideum
Retroposons: Genetic Footprints on the Evolutionary Paths of Life
Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors
Visualization of Complex Processes in Lipid Systems Using Computer Simulations and Molecular Graphic
The Use of RNA Probes for the Analysis of Gene Expression: Northern Blot Hybridization and Ribonucle
Directed Differentiation of Dopamine Neurons from Human Pluripotent Stem Cells
Lineage Selection for Generation and Amplification of Neural Precursor Cells
Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity
Analysis of Skeletal Muscle Hypertrophy in Models of Increased Loading
Crystallization and Crystal Structure Determination of Ribonuclease A-Ribonuclease Inhibitor Protein
Lipidomic Analysis of Human Meibum Using HPLCMSn
The Use of RAPD for Isolate Identification of Arbuscular Mycorrhizal Fungi
Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells
Colony Cultures: Plating Efficiency Assay and Cloning
Preparation of Feeder Layers And SNL Stocks
In Vivo Transplantation of Genetically Modified Mouse Embryonic Epidermis
Restriction Fragment Length Polymorphism (RFLP) Analyses: Distinguishing African and European Honey
Calcium and Membrane Repair
The Yeast Three-Hybrid System As a Tool to Study Caspases
Freezing Harvested hMSCs and Recovery of hMSCs from Frozen Vials for Subsequent Expansion, Analysis,
细胞生物学名词解释
Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular Membranes
Analysis of the Enantiomers of Chiral Pesticides and Other Pollutants in Environmental Samples by Ca
Isolation of Centrosomes from Cultured Cells
Development and Evaluation of Classifiers
Generation of Lung Epithelial-Like Tissue from hESC by AirLiquid Interface Culture
Humanized Mice for Studying Human Leukocyte Integrins In Vivo
Labeled Stem Cells as Disease Models and in Drug Discovery
Chromosomal Mosaicism in Neural Stem Cells
Expression of Epitope-Tagged Proteins in Arabidopsis Leaf Mesophyll Protoplasts
染色体分带(chromosome banding)
Light-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors
spa Typing for Epidemiological Surveillance of Staphylococcus aureus
ght-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors
Performing Comparative Peptidomics Analyses of Salmonella from Different Growth Conditions
Use of Stem Cell Markers in Dissociated Mammary Populations
Methods for Culture of Human Corneal and Conjunctival Epithelia
Transfection and Transduction of Primary Human Endothelial Cells
A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Prese
Flow Cytometry-Based Apoptosis Detection
Subculture of Adherent Cell Lines
Quantitation of Opioids in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)
Serum Apolipoproteins
Antisense Therapeutics for Hypertension: Targeting the Renin-Angiotensin System
In Vitro Phosphorylation and Kinase Assays in Neurospora crassa
抑郁症的体外模型
Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone
Cell-Penetrating Penta-Peptides and Bax-Inhibiting Peptides: Protocol for Their Application
人抗胰岛细胞抗体(AICA)酶联免疫分析（ELISA）
细胞培养过程中常见问题及其解答
Analysis of Point Mutations by Use of Amber Stop Codon Suppression
High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed
Manufacture of Clinical Grade Human Placenta-Derived Multipotent Mesenchymal Stromal Cells
Microarray Analysis
Measuring DNA Precursor Pools in Mitochondria
Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly
Interface Organotypic Hippocampal Slice Cultures
Freezing Cells
肌纤维(myofibers)
Mutagenesis With Drosophila
RNA Extraction From Mammalian Tissues
PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes
Tissue-Selective Knockouts of Steroid Receptors: A Novel Paradigm in the Study of Steroid Action
绒毛染色体制备方法
FISH on Plant Chromosomes
Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotides
Computational Reconstruction of Ancestral DNA Sequences
Characterization of Genes for Polyamine Modulon
Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture
Assessing Sequence-Specific DNA Binding and Transcriptional Activity of STAT1 Transcription Factor
Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells
T Cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T Cells in Chronic Inte
Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis
Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications
Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay
Assessment of Apoptotic Cell Phagocytosis by Macrophages
Mapping Protein-DNA Interactions Using In Vivo Footprinting
Assessment of Apoptotic Cell Phagocytosis by Macrophages
Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay
Strategies for Imaging Microtubules in Plant Cells
Eradication of Mycoplasma Contaminations
Exploring Polyamine Biosynthetic Diversity Through Comparative and Functional Genomics
Immunocytochemical Techniques for Studying Apoptosis in Primary Sympathetic Neurons
后期(anaphase)
Isolation and In Vitro Binding of Mating Type Plus Fertilization Tubules From Chlamydomonas
Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides
Humanized Mouse Models to Study the Human Haematopoietic Stem Cell Compartment
Handmade Somatic Cell Cloning in Cattle
Drosophila melanogaster and the Development of Biology in the 20th Century
A Simple Crosslinking Method, CLAMP, to Map the Sites of RNA-Contacting Domains Within a Protein
RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells
Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium
Assay of Deoxyhypusine Synthase Activity
核孔运输(transport through nuclear pore)
Electrotransfection with Intracellular Buffer
Multidimensional Proteomic Analysis of Proteolytic Pathways Involved in Cell Cycle Control
Effects of Rho Family GTPases on Cell-Cell Adhesion
中心体
Modulation of Potassium Ion Channel Proteins Utilising Antibodies
Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation
Purification of Murine Monoclonal Antibodies
Characterization of Enzyme Activity, Protein Content, and Thiol Groups in Immobilized Enzymes
Oocyte Isolation and Enucleation
Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein
Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization
Detection of Apoptosis in the Central Nervous System
Serum-Resistant Lipoplexes in the Presence of Asialofetuin
Isolation and Characterization of Primitive Hematopoietic Cells Based on Their Position in the Cell
Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading
Epithelial-Mesenchymal Interactions
自由基(free radical)
DNA Microarray for Molecular Epidemiology of Salmonella
Nuclear Reprogramming of Somatic Nucleus Hybridized With Embryonic Stem Cells by Electrofusion
Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis
液泡(vacuoles)
Generation of Transgene-Free iPSC Lines from Human Normal and Neoplastic Blood Cells Using Episomal
Developmental Biology Protocols: Overview I
Immunogold Labelling for Scanning Electron Microscopy
Human Long-Term Culture Initiating Cell Assay
Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Li
Electroporation Strategies for Genetic Manipulation and Cell Labeling
Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development
Primary Marrow-Derived Stromal Cells: Isolation and Manipulation
Transplantation of Human Embryonic Stem Cells to the Chick Embryo
Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography,
缝隙连接（gap junction）
Microinjection into Xenopus Oocytes and Embryos
M-Current Recording from Acute DRG Slices
前质体(proplastid)
The Problems and Pitfalls of Radioligand Binding
Differentiation of B Lymphocytes From Hematopoietic Stem Cells
贴壁细胞培养过程中支原体污染的几种救治方案
High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation
Disrupting Protein Complexes Using Tat-Tagged Peptide Mimics
细胞培养目的与用途
细胞培养器械的清洗与消毒方法
细胞生物基本方法：肌组织细胞培养
Autophagy in Disease
Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research
Mammalian In Vitro Splicing Assays
核剪接(nuclear splicing)
Creating a Hopeful Monster: Mouse Forward Genetic Screens
猴白细胞介素-4（IL-4）酶联免疫分析（ELISA）
Activation of p38 and Determination of Its Activity
细胞培养基的基本成分
Cell and Tissue Transplantation in Zebrafish Embryos
Direct PCR Sequencing with Denaturants (Formamide)
Long-Term B Lymphoid Cultures from Murine Bone Marrow: Establishment and Cloning by Using Stromal Ce
Generation of Inner Ear Cell Types From Embryonic Stem Cells
Fluorescent Biosensors for the Detection of HMGB1 Release
Media Composition: Amino Acids and Cellular Homeostasis
Overview
Light Microscopy to Image and Quantify Cell Movement
Culture of BEND Cells (Bovine Endometrial Cells)
乳制品中激素的快速检测
线粒体膜蛋白与细胞凋亡
Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Embryos Usi
Methods to Detect Apoptotic-Like Cell Death in Filamentous Fungi
Methods to Analyze the Homing Efficiency and Spatial Distribution of Hematopoietic Stem and Progenit
Immunolocalization of Poly ADP-Ribose on Drosophila Polytene Chromosomes
Hematopoietic Stem Cell Mobilization with G-CSF
Incorporation of Genetically Modified Cells in Chicken Chimeras
Quantitative Assessment of Pheromone-Induced Dauer Formation in Caenorhabditis elegans
Tube Formation: an In Vitro Matrigel Angiogenesis Assay
Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID
Large-Scale Mitotic Cell Synchronization
Cartilage Proteoglycan Release Assay
Isolation of Song-Regulated Genes in the Brain of Songbirds
Chick Embryos: Incubation and Isolation
Identification of Neural Stem Cells in the Drosophila Larval Brain
转细胞运输(transcellular transport)
Immunogold Labeling for Electron Microscopy
Lentiviral Gene Transduction of Mouse and Human Stem Cells
Application of Functional Blocking Antibodies: N-Cadherin and Chick Embryonic Limb Development
The Mammalian Cell Cycle: An Overview
Ultrastructural Analysis of Myoblast Fusion in Drosophila
Determination of Fatty Acid Profiles and TAGs in Vegetable Oils by MALDI-TOF/MS Fingerprinting
Manual Methods for Sperm Motility Assessment
Identification and Characterization of Programmed Cell Death Markers in Bacterial Models
Preparation of Substrata for In Vitro Culture of Neurons
Cloning Differentially Expressed Genes by Using Differential Display and Subtractive Hybridization
Genomic Amplification with Transcript Sequencing (GAWTS)
Chondrogenic Differentiation of Menstrual Blood-Derived Stem Cells on Nanofibrous Scaffolds
MAPK Signaling Pathway-Aviva
Claudin-16/Paracellin-1, Cloning, Expression, and Its Role in Tight Junction Functions in Cancer and
Protein Extraction From Drosophila Heads
Defrosting Eukaryotic Cells Frozen In Liquid N2
形态建成(morphogenesis)
Live Imaging of Bone Cell and Organ Cultures
The Molecular Identification of Trypanosomes
Epigenetic Control in Male Germ Cells: A Transillumination-Assisted Microdissection Method for the A
Expanding Human T Regulatory Cells with the mTOR-Inhibitor Rapamycin
Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-P
The Use of In Vivo Microdialysis Techniques to Detect Extracellular ROS in Resting and Contracting S
Investigations of Neuronal Migration in the Central Nervous System
Integrins and Vascular Development in Differentiated Embryonic Stem Cells In Vitro
Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders
Protein Extraction, Fractionation, and Purification From Cyanobacteria
Predicting Riboswitch Regulation on a Genomic Scale
Whole Cell Entrapment Techniques
Acid-Labile Liposome/pDNA Complexes
Lentiviral Vector-Mediated Transgenesis in Human Embryonic Stem Cells
Purification of PCR Products from Agarose Gels for Direct Sequencing
水稻根尖染色体标本制备
Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography
Ex Vivo Expansion of Haematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation
Sequencing of Antibodies
How do I decontaminate my tissue culture
Peptide-Induced Fusion of Liposomes
Modeling Spatial and Temporal Dynamics of Chemotactic Networks
Site-Specific Cleavage of Fusion Proteins
Preparation of Low Density, Collagenase-Digested Splenocytes (Hedrick Lab, UCSD Cancer Center)
A Practical Guide for using the AdEasy System
Transfection of Osteoclasts and Osteoclast Precursors
Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules
Method: Reactivating Cell Lines and Cell Growth for DNA Preparation
Site-Directed Mutagenesis in the Study of Membrane Transporters
Analysis of Proteins from Marine Molluscs
The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Huma
The Use of Calorie Restriction Mimetics to Study Aging
Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures
MMP-9 and TIMP-1 Assays in Keratinocyte Cultures
Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues
PKA系统(protein kinase A system, PKA)
Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems
Measurement of Caspase Activity in Cells Undergoing Apoptosis
Cell Fusion Assays for Yeast Mating Pairs
Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis
Flow Cytometric Analysis of Human Pluripotent Stem Cells
核糖体(ribosome)
Quantifying Hematopoietic Stem and Progenitor Cell Mobilization
Capillary Electrophoresis Separation of Microorganisms
几种常用的细菌计数方法
Protein Expression and Purification of Integrin I Domains and IgSF Ligands for Crystallography
Cell Fusion in Caenorhabditis elegans
Single Channel Analysis of Membrane Proteins in Artificial Bilayer Membranes
ERK-MAP Kinase Signaling in the Cytoplasm
Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics
Adhesion, Migration, and Homing of Murine Hematopoietic Stem Cells and Progenitors
High Sensitivity Measurement of Estrone and Estradiol in Serum and Plasma Using LC-MS/MS
Silencing in Yeast: Identification of Clr4 Targets
Use of the Hollow Fibre Assay for Studies on Tumor Neovasculature
Knockout Mice and Steroid Receptor Research
Ultrastructural Analysis of Cell Fusion in Yeast
Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry
A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-cultures
Monitoring of Mycophenolate Acid in Serum or Plasma Using LC Tandem Mass Spectrometry
Analysis of Changes in Protein Level and Subcellular Localization During Cell Cycle Progression Usin
Purification of Recombinant Poly(ADP-Ribose) Polymerases
de Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination
3D Sample Preparation for Orthopaedic Tissue Engineering Bioreactors
Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing
Efficient Gene Knockdowns in Human Embryonic Stem Cells Using Lentiviral-Based RNAi
Toxicities of Mobilized Stem Cell Infusion
光吸收(light absorption)
Analysis of Phospholipids by High Performance Liquid Chromatography
Isolation and Culture of Primary Chicken Osteoclasts
Gene Transfer Via Nucleofection Into Adult and Embryonic Stem Cells
Immunoprecipitation Analysis to Study RNA-Protein Interactions in Xenopus Oocytes
Proteomic Analysis of Dystrophic Muscle
人末梢血微量全血培养染色体显示法
The Myc World Within Reach
Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic M
Preparative Electrophoresis: Extraction of Nucleic Acids From Gels
The 25A/RNase L Pathway and Inhibition by RNase L Inhibitor (RLI)
Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display
Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease
细胞计数实验中初学者易犯的错误
Primary Culture of Mammalian Taste Epithelium
Whole-Cell Recording Using the Perforated Patch Clamp Technique
Focal Cerebral Ischemia
Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties
Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs
Treatment of Human Pancreatic Tumors Xenografted in Nude Mice by Chemotherapy Combined with Pulsed E
Retroviral Insertion Site Analysis in Dominant Haematopoietic Clones
Enhancer Analysis of the 1(II) and 2(XI) Collagen Genes in Transfected Chondrocytes and Transgenic M
Derivation of Neural Precursors from Bovine Preimplantation Embryos
Gene Transfer Using Targeted Filamentous Bacteriophage
氧化酶(oxidase)
Soft Tissue Reconstruction
Preparation of Fungi for Ultrastructural Investigations and Immunogoldlabelling
pH Sensing Agar Plate Assays for Esterolytic Enzyme Activity
ECM Macromolecules: Height-mapping and Nano-mechanics Using Atomic Force Microscopy
Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplat
电穿孔法进行质体转化步骤
Isolation of Porcine Pancreatic Islets for Xenotransplantation
Production and Purification of Polyclonal Antibodies
High-Resolution Molecular Localization by Freeze-Fracture Replica Labeling
An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue
Assessment of Cardiac Conduction: Basic Principles of Optical Mapping
Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitro
G-蛋白偶联受体(G-protein linked receptor)
Transfection of Adipocytes and Preparation of Nuclear Extracts
Utility of Array CGH in Molecular Diagnosis of Mitochondrial Disorders
Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resin
Simple Experimental and Spontaneous Metastasis Assays in Mice
Functional Genomics by Gene-Trapping in Embryonic Stem Cells
原生质体(potoplast)
Development of ATP-Competitive mTOR Inhibitors
Detecting Expression Patterns of Wnt Pathway Components in Sea Urchin Embryos
Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes
The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins
Measuring Redox Changes In Vivo in Leaves: Prospects and Technical Challenges
Quantitation of Benzodiazepines in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS
A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor
FISH Analysis of Human Pluripotent Stem Cells
Immobilized Metal Ion Affinity Chromatography of Native Proteins
Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction
Measurements of the Intracellular Stability of CPPs
Isolation of Skin Dendritic Cells from Mouse and Man
Application of Fluorescent Measurements for Characterization of RiboswitchLigand Interactions
MicroRNA Knock Down by Cholesterol-Conjugated Antisense Oligos in Mouse Organ Culture
Colorimetric Assays for Screening Laccases
Site-Specific Integration in Human ESC Using Jump-In TI Technology
Preparation and Testing of Synthetic mRNA for Microinjection
Mobilization of Hematopoietic Stem and Progenitor Cells in Mice
Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds
Time-Lapse Imaging of Necrosis
Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from
Transmission Electron Microscopy of Bone
Immune Privilege of Stem Cells
Investigation of Nuclear Envelope Structure and Passive Permeability
Isolation, Identification, and Validation of Microtubule-Associated Proteins from Drosophila Embryos
细胞融合技术的应用研究进展
Assessment of Permeability Barriers to Macromolecules in the Rodent Endometrium at the Onset of Impl
Analysis of the Budding Yeast Saccharomyces cerevisiae Cell Cycle by Morphological Criteria and Flow
Chemical Analysis of Aquatic Pheromones in Fish
Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets
Detection of Impending Graft Rejection and Relapse by Lineage-Specific Chimerism Analysis
Capillary Electrophoresis of Oxidative Dna Damage
Conventional Technologies for Cryopreservation of Human Oocytes and Embryos
Direct Electrical Stimulation of Myogenic Cultures for Analysis of Muscle Fiber Type Control
Single Cell Polymerase Chain Reaction for Preimplantation Genetic Diagnosis: Methods, Strategies, an
Membrane Protein NMR Studies
Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS) Method for Monitoring Methotrexate in the
Visualization of Lipid Domain-Specific Protein Sorting in Giant Unilamellar Vesicles
Microscopic Assessment of Angiogenesis in Tumors
Visualization of Adult Stem Cells Within Their Niches Using the Drosophila Germline as a Model Syste
The Direction of Ribonucleases H by Antisense Oligodeoxynucleotides
端粒顺序(telomeric sequence，TEL)
Detection of Calcium Release via Ryanodine Receptors
狗的克隆
Human Peripheral Blood Mononuclear Cell Preparation
Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration
Culture of Human Brain Tumors on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cel
c-Myb in Smooth Muscle Cells
Umbilical Cord Blood Stem Cells for Myocardial Repair and Regeneration
人工染色体(artificial chromosome)
Immobilization of Whole Cells by Chemical Vapor Deposition of Silica
Isolation and Enrichment of Stro-1 Immunoselected Mesenchymal Stem Cells from Adult Human Bone Marro
Detection of Uncoupling Protein-2 (UCP2) As a Mitochondrial Modulator of Apoptosis
Diagonal Electrophoresis for Detection of Protein Disulphide Bridges
实用哺乳动物细胞培养手册
Cell-Free Antibody Capture Method for Analysis of Detergent-Resistant Membrane Rafts
核糖核酸聚合酶（RNA polymerase）
FISH With and Without COT1 DNA
异固缩(heteropythosis)
Urine Proteins
Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Cho
Design and Analysis of Experiments
Bioinformatics Strategies for the Analysis of Lipids
Computer-Aided Sperm Analysis (CASA) of Sperm Motility and Hyperactivation
Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal Cells
Interspecific Mouse-Chick Chimeras
Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis
Screening for Epigenetic Target Genes that Enhance Reprogramming Using Lentiviral-Delivered shRNA
Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable a
ProteinProtein Interaction Techniques: Dissect PCP Signaling in Xenopus
Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Imm
Gel-Based and Gel-Free Proteomic Technologies
Nuclear Transfer in Nonhuman Primates
Nanosizing by Spatially Modulated Illumination (SMI) Microscopy and Applications to the Nucleus
Imaging Plasma Membrane Proteins by Confocal Microscopy
Homologous Gene Targeting to Study ECM Assembly
Assay for Protein Modification by Poly-ADP-Ribose In Vitro
Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Supp
Expression of EGF and HIV Envelope Glycoprotein
Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry
Extrachromosomal Assay for DNA Double-Strand Break Repair
Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Indiv
Direct Sequencing of PCR Products Using Chemiluminescent Detection
Live Imaging of LFA-1-Dependent T-Cell Motility and Stop Signals
Computer-Assisted Interpretation of Triacylglycerols Mass Spectra
胶原(collagen)
Group Migration and Signal Relay in Dictyostelium
Purifying the Hedgehog Protein and its Variants
Quantitative Visualization of Autophagy Induction by mTOR Inhibitors
Studying Early Hematopoiesis Using Avian Blastoderm Cultures
125I-UdR致C6胶质瘤细胞损伤的模型
Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector
Cationic α-Helical Peptides for Gene Delivery into Cells
Lipoplex Formation Using Liposomes Prepared by Ethanol Injection
植物油凝素的制备
Metalloproteases and the Degradome
Protocol for evaluating vascular permeability in wildtype
动物体内转染试剂
特征性研究：内源性GPCRs 细胞功能图研究
Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementation of Yeast
Preparing 48-Well Plate Feeders
LINE-1 Elements: Analysis by Fluorescence In-Situ Hybridization and Nucleotide Sequences
cos-7的转染方法讨论
Embryonic Stem Cells for Osteo-Degenerative Diseases
Development of Hematopoietic Stem and Progenitor Cells from Mouse Embryonic Stem Cells, In Vitro, Su
Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions
Cell Counting/ Live-Dead Discrimination
Sperm Selection Based on Electrostatic Charge
A Double Nuclear Transfer Technique for Cloning Pigs
Nat.Methods：新型悬浮培养技术可大规模生产干细胞
In Vivo Gene Electroporation in the Mouse Testis
Adult Ventricular Cardiomyocytes: Isolation and Culture
Analyzing DNA Replication Dynamics of Genotoxin-Treated Cells Using Velocity Sedimentation
Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes
Purification of MMPs and TIMPs
Detecting sRNAs by Northern Blotting
Ca2+ Imaging as a Tool to Assess TRP Channel Function in Murine Distal Nephrons
Regulation of Convergent Extension by Non-canonical Wnt Signaling in the Xenopus Embryo
Use of Codon Cassette Mutagenesis for Saturation Mutagenesis
Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos: Optimal Ring and Rol
Mitochondrial DNA Mutations: An Overview of Clinical and Molecular Aspects
Analysis and Classification of Serum Cryoglobulins
Studying Proteolysis Within Mitochondria
Progenitor Cell Mobilization from Extramedullary Organs
Electron Microscopic Imaging of Integrin
Determination of Carbonyl Groups in Oxidized Proteins
Equilibrium Binding and Transport Studies
Reprogramming of Committed Lymphoid Cells by Enforced Transcription Factor Expression
Generation of Chondrocytes From Embryonic Stem Cells
Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo
结晶紫染色法测定细胞数
In Silico Identification of Novel G Protein Coupled Receptors
Doxycycline Regulated Lentiviral Vectors
Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos
Large Animal Model of Heart Failure for Assessment of Stem Cells
嵴(cristae)
Angiogenesis Assays in the Chick CAM
Pharmacogenetics: Defining the Genetic Basis of Drug Action and Inositol Trisphosphate Analysis
Analysis of RNA Structure and RNA-Protein Interactions in Mammalian Cells by Use of Terminal Transfe
Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow
Human Myometrial Smooth Muscle Cells and Cervical Fibroblasts in Culture: A Comparative Study
Analysis of Tubulin Transport in Nerve Processes
RNAi in the Hedgehog Signaling Pathway: pFRiPE, a Vector for Temporally and Spatially Controlled RNA
Purification and Identification of Protein Complexes That Control the Cell Cycle
In Ovo Gene Electroporation into Early Chicken Embryos
Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters
Identification, Characterization, and Differentiation of Human Prostate Cells
Biochemical Methods to Determine Cell-Surface Topography: Part A. Labeling of Oxidized Glycoproteins
Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes
Generation of Mouse Embryos with Small Hairpin RNA-Mediated Knockdown of Gene Expression
Potential Zoonotic Infection of Porcine Endogenous Retrovirus in Xenotransplantation
Microsomal Proteomics
Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation
Simultaneous Immunofluorescence Detection of Cadherin and -Catenin in Mouse Tissue Sections
Hepatic Maturation of hES Cells by Using a Murine Mesenchymal Cell Line Derived from Fetal Livers
人胰岛β细胞酶联免疫分析（ELISA）
Large-Scale Production and Storage of Monoclonal Antibodies and Hybridomas
Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
Using Immuno-Scanning Electron Microscopy for the Observation of Focal Adhesion-substratum interacti
Imaging Cell Movement During Dorsal Closure in Drosophila Embryos
Optical Control of Ligand-Gated Ion Channels
C-Terminal Sequence Analysis of 2DE-Separated Proteins
Amino Acid Regulation of Autophagosome Formation
Cell-Surface ProteinProtein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies:
Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro
γ微管蛋白(γ tubulin)
In Vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation
Activation and Function of Small GTPases Rho, Rac, and Cdc42 During Gastrulation
Heart Xenotransplantation in Primate Models
cDNA Subtraction and Cloning in the Field of Trophoblast/Placental Development
Treatment of a Tumor Model with ECT Using 4+4 Electrode Configuration
配子发生(gametogenesis)
细胞转染后缩为小圆点
Analysis of MMP-Dependent Cell Migration and Invasion
Random Mutagenesis by Using Mixtures of dNTP and dITP in PCR
Quantitative In Situ Analysis of Claudin Expression at the BloodRetinal Barrier
Synchronization of HeLa Cells
Determination of Aliphatic Low-Molecular-Weight and Biogenic Amines by Capillary Zone Electrophoresi
Isolation of Satellite Cells from Single Muscle Fibers from Young, Aged, or Dystrophic Muscles
Silver Sequencing: Nonradioactive Cycle Sequencing of dsDNA
Methodology, Biology and Clinical Applications of Human Mesenchymal Stem Cells
DNA Fragmentation Assays for Apoptosis Protocol
Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-Dimensional Collagen Matrix
Embryonic Stem Cell Differentiation and the Vascular Lineage
Evaluation of Cell Morphology
Use of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent Blood Coagulat
Insulin-Producing Cells from Embryonic Stem Cells Experimental Considerations
Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation
Reconstitution of the Nuclear Transport of the MAP Kinase ERK2
Use of Firefly Luciferase Activity Assays to Monitor Circadian Molecular Rhythms In Vivo and In Vitr
Investigation of KATP Channel Endocytosis by Immunofluorescence
Short-Term Chorionic Villi and Amniotic Fluid Cultures
Internalization of Nucleoside Phosphates into Live Cells by Complex Formation with Different CPPs an
Monitoring Sterol Uptake, Acetylation, and Export in Yeast
Elastic Liposomes for Topical and Transdermal Drug Delivery
New Opportunities for Immobilization of Enzymes
Flow Cytometry Analysis of Cell Cycling and Proliferation in Mouse Hematopoietic Stem and Progenitor
Immunofluorescence Analysis of Circadian Protein Dynamics in Cultured Mammalian Cells
Design of Experiments to Investigate Dynamic Cell Signaling Models
Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodies
CD8+, CD8, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand
Bone Marrow-Derived Mesenchymal Stem Cells: Isolation, Expansion, Characterization, Viral Transducti
MTT方法的使用步骤
糖原的显示―PAS反应（periodic acid schiff’s reaction）
Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC)
Using 2D Crystals to Analyze the Structure of Membrane Proteins
细胞培养基中常用维生素及其作用
Cloning of Potassium Channel Splice Variants from Tissues and Cells
Immunohistochemistry of Xenopus Embryos
Isolation of Hair Follicle Bulge Stem Cells from YFP-Expressing Reporter Mice
Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts
Functional Assays for Hematopoietic Stem Cell Self-Renewal
Biomarkers of Cell Senescence Assessed by Imaging Cytometry
PMEF FEEDER LAYER CONCENTRATION
Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue
ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/M
The Intranuclear Environment
Culture Systems: Mineral Oil Overlay
Studying Mitochondria in an Attractive Model: Schizosaccharomyces pombe
An Integrated Approach for Monitoring Cell Senescence
Analysis of Cell Generation in theTelencephalic Neuroepithelium
染色体扫描电镜标本制备与观察
Characterization of Calcium/Calmodulin- Stimulated Protein Kinase II
Measurement of Calcium Release Due to Inositol Trisphosphate Receptors in Skeletal Muscle
Adipogenic Differentiation of Human Mesenchymal Stem Cells
Lentiviral Vectors to Study the Differential Function of ERK1 and ERK2 MAP Kinases
Methods for Constructing and Producing Retroviral Vectors
Human Peripheral Blood Mononuclear Cell Preparation
Culture and Differentiation of Mouse Tracheal Epithelial Cells
A High Content Imaging-Based Approach for Classifying Cellular Phenotypes
人酸性成纤维细胞生长因子（aFGF）酶联免疫分析（ELISA）
细胞骨架系统(cytoskeletonic system)
Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresi
细胞分化(cell differentiation)
Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative G
Using Endogenous MicroRNA Expression Patterns to Visualize Neural Differentiation of Human Pluripote
Human Vascular Smooth Muscle Cell Culture
Identification of Differentially Expressed Fungal Genes In Planta by Suppression Subtraction Hybridi
Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death
High-Throughput Screening of Mutant -Amylase Libraries for Increased Activity at 129C
Characterization of Cellular Internalization Pathways for CPP-Mediated Oligonucleotide Delivery
In Vitro Study of Nuclear Assembly and Nuclear Import Using Xenopus Egg Extracts
推荐：细胞冻存步骤的专业实验流程
Spermatogonial Stem Cells
Crystal Violet Assay
Three-Dimensional Cultures of Mouse Mammary Epithelial Cells
Microtubule Spindowns for Visual Analysis
稳定转染中的问题:从G418浓度确定到单克隆化鉴定
转染的时候能否用加血清的培养基
Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis
主动运输
实验室各种板子的用法比较
Mimicry Technology: Suppressing Small RNA Activity in Plants
核膜
细胞凋亡有什么特点？
ARS 序列(autonomous replicating sequence)
Novagen公司推出无需IPTG的自动诱导大肠杆菌培养基
In Vitro Stimulation and Detection of IFN Production in Human Plasmacytoid Dendritic Cells
Determination of Oxidized and Reduced CoQ10 and CoQ9 in Human Plasma/Serum Using HPLC-ECD
Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions
Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Str
Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Mem
Endogenous Inhibitory Mechanisms and the Regulation of Platelet Function
关于细胞培养基的几点讨论
Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques
Resources for Genetic and Genomic Studies of Xenopus
The Isolation of Nuclei and Nuclear Membranes from Rat Liver
Functional Analysis of Cell Adhesion Molecules
Liposomal siRNA Delivery
Single-Molecule Methods to Study Cell Adhesion Molecules
Detection and Measurement of Necrosis in Plants
Fluorescence Methods in the Study of Small GTP-Binding Proteins
Antisense Strategies for Treatment of Heart Failure
Gene Expression and Methylation Patterns in Cloned Embryos
Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine
Cell Sorting By FACS
Identifying Myc Interactors
Flow Cytometric Analysis of Drosophila Cells
Measurement of Ion Fluxes and pH Gradients Across Cell Membranes
猪白细胞介素-8（IL-8）酶联免疫分析（ELISA）
山羊脑炎（encephalitis）酶联免疫分析（ELISA）
Applications of Cell-Penetrating Peptides as Signal Transduction Modulators for the Selective Induct
Antisense Therapy of Hepatitis B Virus Infection: In Vivo Analyses in the Duck Hepatitis B Virus Mod
Optimizing Electroporation Conditions for Intracellular Delivery of Morpholino Antisense Oligonucleo
Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis
Assessing Organismal Aging in the Filamentous Fungus Podospora anserina
End-Labeling and Analysis of Spo11-Oligonucleotide Complexes in Saccharomyces cerevisiae
Use of Differentiating Embryonic Stem Cells in the Parkinsonian Mouse Model
Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Biolu
Screening for -Ray Hypersensitive Mutants of Arabidopsis
Cellulose Paper Support for Immobilization
The Dual Use of RNA Aptamer Sequences for Affinity Purification and Localization Studies of RNAs and
Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora
流式细胞术分析血小板
Neural Cell Lines
Quantitative Studies of Neuronal Chemotaxis in 3D
The IUBMB-Endorsed Transporter Classification System
Uses of Telomerase Peptides in Anti-Tumor Immune Therapy
培养293细胞应注意的几个问题
Markers of Cellular Senescence
出胞与入胞
融合蛋白(fusion protein)
Measurement of Filter Paper Bloodspot Lead by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)
A convenient method for the isolation of crude nuclear pellets.
联合作图（association mapping）和单倍体型（haplotype）
扫描隧道显微镜(scanning tunneling microscope，STM)
Wnt-Frizzled Interactions in Xenopus
Spermatogenesis in Cryptorchidism
Analysis of Two Aspects of Left-Right Patterning of the Vertebrate Heart: Heart Tube Position and He
Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Livi
Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts
Hematopoietic Progenitor Cell Apheresis Processing
动物细胞大规模培养用生物反应器的简介与分类
Probing mRNA Structure and sRNAmRNA Interactions in Bacteria Using Enzymes and Lead(II)
Continuous Infusion of Antisense Phosphorothioate Therapeutics
The Measurement of Phospholipase D- Linked Signaling in Cells
Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Cand
Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells
Robust Multiparametric Assessment of Cellular Senescence
Measurement of Cyclic GMP Formation
Directed Differentiation of Pluripotent Stem Cells to Functional Hepatocytes
Steroid Analysis by Micellar Electrokinetic Capillary Chromatography
Analysis of the C. elegans Germline Stem Cell Region
Selection of Peptides Interfering with ProteinProtein Interaction
Localized Sampling, Electrophoresis, and Biosensor Analysis of Xenopus laevis Cytoplasm for Subcellu
Double-Stranded DNA Site-Directed Mutagenesis
History of Antisense Oligonucleotides
Transformation of Fish Cells and Embryos
In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast
细胞调亡的研究方法：琼脂糖凝胶电泳
Plasmid Replicon Typing
Isolation and Manipulation of Mammalian Neural Stem Cells In Vitro
Micro-structured Materials and Mechanical Cues in 3D Collagen Gels
Functional Expression of CXCR4 in Saccharomyces cerevisiae in the Development of Powerful Tools for
Interleukin-27 Induces Interferon-Inducible Genes: Analysis of Gene Expression Profiles Using Affyme
Detection and Activation of Stress-Responsive Tyrosine Kinases
ProteinProtein Interactions in Signaling Cascades
细胞培养基的基本要求、组成及作用
Measurement of Apoptosis by DNA Fragmentation
The Use of PARP Inhibitors in Cancer Therapy: Use as Adjuvant with Chemotherapy or Radiotherapy; Use
L-selectin-PNAd Interactions under Flow Conditions.
Cell Clonogenic Survival Assay
细胞培养中常见的污染情况
细胞培养的一般技术
Flow Cytometric Analysis Of Bcl Family members
电转感受态菌TG1的制备
Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approac
Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts a
干细胞的冻存方法与细胞计数
新生牛血清的筛选
荧光激活的细胞分类仪检测调亡细胞
什么是细胞凋亡？
关于小鼠腹腔巨噬细胞的几个问题
Protocol for intracytoplasmic staining of cytokines for FACS analysis
人可溶性白细胞分化抗原40配体（sCD40L）酶联免疫分析
人源TCTP可调控mTOR信号通路
Tube formation assay
红细胞比容（hematocrit value）
Growing feeder-independent embryonic stem cells§
Measuring mtDNA Damage Using a Supercoiling-Sensitive qPCR Approach
Tumor Cell Invasion Assays
A Novel Method for the Production of Fully Modified K-Ras 4B
Genome-Wide Overexpression Screen for Activators of Antioxidant Gene Transcription
Chemotaxis Assay
酸性磷酸酶法检验细胞增殖
Retroviral Gene Transduction in Limb Bud Micromass Cultures
Retroviral Integration Site Analysis in Hematopoietic Stem Cells
Resonance Raman Spectroscopy of Metalloproteins Using CW Laser Excitation
Canine Models of Gene-Modified Hematopoiesis
Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of RNA
Isolation and Culture of Mouse Intestinal Cells
Examination of Normal and Abnormal Placentation in the Mouse
流式细胞仪主要技术指标
Transfection and Transformation of Human Thyroid Epithelial Cells
细胞粘着(cell adhesion)
$冰冻断裂(freeze fracture)$
冰冻断裂(freeze fracture)
Coculture of Decidua and Trophoblast to Study Proliferation and Invasion
Preparation and Analysis of Uniquely Positioned Mononucleosomes
Screening of Telomerase Inhibitors
Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Gerany
核仁周期(nucleolar cycle)
The Introduction of Proteins into Mammalian Cells by Electroporation
PCR Sequencing with the Aid of Detergents
Real-Time Imaging of Meiotic Chromosomes in Saccharomyces cerevisiae
Methods for Culturing Human Embryonic Stem Cells on Feeders
科学委员会支持南非建最大射电望远镜
Hematopoietic Development of ES Cells in Culture
Nuclear Transplantation in Xenopus
Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization
Imaging Actin Cytoskeleton Dynamics in Dictyostelium Chemotaxis
Analysis of B-Cell Life-Span and Homeostasis
Detergent-Extracted Models for the Study of Cilia or Flagella
The Voltage-Clamp Fluorometry Technique
Remote Loading of Anthracyclines into Liposomes
Cell Lineage Analysis: Applications of Green Fluorescent Protein
Microtubule Binding Assays
Detection of Nuclear Envelope Alterations in Senescence
A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction
母体信息（maternal information）
In situ Hybridization with RNA Probes
Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological condi
Immunostaining of Skeletal Tissues
Analysis of tRNA-Directed Transcription Antitermination in the T Box System In Vivo
Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study
Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies
Histopathologic Assessment of Myocardial Regeneration
Mitochondrial Respiratory Chain: Biochemical Analysis and Criterion for Deficiency in Diagnosis
An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations
Methods for Analysis of Crossover Interference in Saccharomyces cerevisiae
Generation of a Vascular Niche for Studying Stem Cell Homeostasis
Cell Kinetic Analysis in Artificial Skin Using Immunochemical Methods
Effective In Vitro and In Vivo Gene Delivery by the Combination of Liposomal Bubbles (Bubble Liposom
T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences
Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display
A Systems Approach Demonstrating Sphingolipid-Dependent Transcription in Stress Responses
Detection and Quantification of Cytokines and Other Biomarkers
Preparation of GTPases for Structural and Biophysical Analysis
Quantification of Human Cytomegalovirus by Competitive PCR and Capillary Electrophoresis
Detection of Human and Mouse Granzyme B Activity in Cell Extracts
Cloning PCR Products for Sequencing in M13 Vectors
Isolation of Neuroepithelium and Formation of Minispheres
Culture of Human Renal Medullary Interstitial Cells
Atomic Force Microscopy for Analyzing Metaphase Chromosomes: Comparison of AFM Images with Fluoresce
Designing and Utilization of siRNAs Targeting RNA Binding Proteins
Fluorescent Transgenes to Study Interphase Chromosomes in Living Plants
Immunofluorescence Methods in Studies of the GTPase Ran and Its Effectors in Interphase and in Mitot
Cryopreservation of the Sperm of the Pacific Oyster Crassostrea gigas
Basic Analyses of Proteins of Interest for Xenotransplantation
Wingless Signaling in Drosophila Eye Development
A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene
Protein Separation Using Immobilized Phospholipid Chromatography
Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism
Epiggenetics in Dictyostelium
Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells
In Vitro Maturation and In Vitro Fertilization of Human Oocytes
Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation
Frizzled-7-dependent Tissue Separation in the Xenopus Gastrula
Construction of Linker-Scanning Mutations Using PCR
Localization and MHC Class II Presentation of Antigens Targeted for Macroautophagy
Navigation to the Graveyard-Induction of Various Pathways of Necrosis and Their Classification by Fl
Isotope Labeling Strategies for Analysis of an Ion Channel Cytoplasmic Domain by NMR Spectroscopy
Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids
Dissecting Nucleases into Their Structural and Functional Domains: Mapping the RNA-Binding Surface o
TEM Visualization of Microtubules
Zymography and Reverse Zymography for Detecting MMPs, andTIMPs
Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts
Studying Vascular Progenitor Cells in a Neonatal Mouse Model
Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Mu
Synthesis of Polyampholyte Comb-Type Copolymers Consisting of a Poly(L-lysine) Backbone and Hyaluron
Isolation of Rat Intestinal Lymph DC
Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
Studying the Mechanosensitivity of Voltage-Gated Channels Using Oocyte Patches
Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separ
Adipose-Derived Stem Cells for Periodontal Tissue Regeneration
Measurements of Glucose Conversion to its Metabolites
Stabilization of Polycation-DNA Complexes by Surface Modification with Hydrophilic Polymers
Isolation, Culture, and Characterization of Human Umbilical Cord Stroma-derived Mesenchymal Stem Cel
Characterization of STAT Self-Association by Analytical Ultracentrifugation
DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73)
An Easy and Reliable Automated Method to Estimate Oxidative Stress in the Clinical Setting
In Vitro and In Vivo Protein Import Into Plant Mitochondria
Generating stable cell lines in HEK293
Cell Cycles - Introduction
Protein Techniques: Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting
293细胞的大规模培养三种方式
Isolation and Characterization of Distal Lung Progenitor Cells
类囊体(thylakoid)
Electron Microscope Visualization of RNA Transcription and Processing in Saccharomyces cerevisiae by
An In Vitro Assay for the Kinase Activity of mTOR Complex 2
Protein-Thiol Oxidation, From Single Proteins to Proteome-Wide Analyses
Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems
Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Gr
Treatment of Multiple Spontaneous Breast Tumors in Mice Using Electrochemotherapy
Isolation and Culture of Rabbit Embryonic Stem Cells
Transgenic Systems in Nuclear Reprogramming
Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High-Densit
Genetic Manipulation of Keratinocyte Stem Cells with Lentiviral Vectors
Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aort
Studying Nuclear Reprogramming with Cell Hybrids
The Identification of Subcellular Fractions from Mammalian Cells
Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
Methods for Studying Checkpoint Kinases Chk1
Prespliceosome and Spliceosome Isolation and Analysis
Long-Term B-Lymphoid Cultures from Murine Bone Marrow Establishment and Cloning by Using Stromal Cel
Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software
GeneCalling: Transcript Profiling Coupled to a Gene Database Query
Deep Sequencing of Small Chromatin-Associated RNA: Bioinformatic Analysis
Recognition Imaging of Chromatin and Chromatin-Remodeling Complexes in the Atomic Force Microscope
Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines
The Calvarial Injection Assay
Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Cult
愈伤组织(callus， culli)
Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes
Generation of Human Melanocytes from Induced Pluripotent Stem Cells
Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells
Feeder-Layer Free Culture System for Human Embryonic Stem Cells
A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered
Media Composition: Salts and Osmolality
Bioreactor Cultivation of Functional Bone Grafts
Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment L
Trophoblast Differentiation: An In Vitro Model for Trophoblast Giant Cell Development
Direct Sequencing of PCR Products with DNA-Binding Proteins
Visualization of mRNA Expression in the Zebrafish Embryo
Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines
Fourier Transform Infrared Imaging of Bone
Transient Overexpression of Genes in Neurons Using Nucleofection
Isolation and Culture of Mouse Satellite Cells
Differential mRNA Display: Adaption for In Vivo Studies of Diseased Tissues
Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes
Quantification of Cyclobutane Pyrimidine Dimers in Human Epidermal Stem Cells
HPLC/MS/MS-Based Approaches for Detection and Quantification of Eicosanoids
初级溶酶体(primary lysosome)
Assays of Adrenergic Receptors: Including Lipolysis and Binding Measurements
Role of Endothelial CellCell Junctions in Endothelial Permeability
Purification of Plant Ribosomal DNA: Use of Buoyant Density Centrifugation in Cesium Chloride-Actino
Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports
Visualization and Interpretation of Eukaryotic DNA Replication Intermediates In Vivo by Electron Mic
Rat Brain Neuropeptidomics: Tissue Collection, Protease Inhibition, Neuropeptide Extraction, and Mas
Generation of Tissue Organoids by Compaction Reaggregation
Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Ma
Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs)
Murine Bone Marrow-Derived Macrophages
纺锤体（spindle）
Sos蛋白(Sos protein)
Melanosome Motility in Fish Retinal Pigment Epithelial Cells
Visualization of Induced RNA in Single Bacterial Cells
Isolation and Short-Term Culture of Human Epidermal Langerhans Cells
Visualization of Spindle Behavior Using Confocal Microscopy
Yeast Protoplast Fusion
Immobilization of Enzymes and Proteins on Red Blood Cells
The Measurement of Protein Degradation in Response to Oxidative Stress
Cell Viability Assays: Introduction
In Situ Hybridization with Nonradioactive Probes
Analysis of Fungal Gene Expression by Real Time Quantitative PCR
Detection of Protein Glutathionylation
导肽(leading peptide)
心肌细胞培养方法详述
Identification of Genes Involved in Tumor-Like Properties of Embryonic Stem Cells
Analysis of Chromatin Composition of Repetitive Sequences: The ChIP-Chop Assay
Review of Molecular Mechanisms Involved in the Activation of the Nrf2-ARE Signaling Pathway by Chemo
Analysis of Fatty Acids by Gas Liquid Chromatography
Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy
In Vivo Imaging of Tight Junctions Using ClaudinEGFP Transgenic Medaka
Culturing Chick Embryonic Cardiac Myocytes and Fibroblasts
Visualization of PCP Defects in the Eye and Wing of Drosophila melanogaster
Cdc28蛋白(Cdc 28 protein)
Acute Behavioral Responses to Pheromones in C. elegans (Adult Behaviors: Attraction, Repulsion)
Measurement of Caspase Activity: From Cell Populations to Individual Cells
Skeletogenesis: In Vitro Analysis of Bone Cell Differentiation
同源异型框(homeobox)
Use of the Cytomegalovirus Promoter for Transient and Stable Transgene Expression in Mouse Embryonic
Manipulation of Gene Function in Xenopus laevis
Comprehensive mRNA Profiling of Lipid-Related Genes in Microglia and Macrophages Using Taqman Arrays
In a magnetofection procedure, self-assembling complexes of enhancers like cationic lipids with plas
Liposomal Magnetofection
Semi-quantitative Analysis of Transient Single-Cell Gene Expression in Embryonic Stem Cells by Femto
Directed Alteration of Saccharomyces cerevisiae Mitochondrial DNA by Biolistic Transformation and Ho
Primary Culture and Propagation of Human Prostate Epithelial Cells
Overview of Integrin Signaling in the Immune System
Analysis of Cell Shape and Polarity During Zebrafish Gastrulation
Staging of Mouse Seminiferous Tubule Cross-Sections
Genetically Encoded Probes Provide a Window on Embryonic Arrhythmia
The Identification of Signaling Molecules by the Yeast Two-Hybrid System
Assays for Chemotaxis and Chemoattractant-Stimulated TorC2 Activation and PKB Substrate Phosphorylat
Generation and Characterization of Murine Alternatively Activated Macrophages
Primary Mouse Small Intestinal Epithelial Cell Cultures
Isolation of Neural Stem and Precursor Cells from Rodent Tissue
Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium
再生(regeneration)
Adult Neural Stem Cells
Detection and Measurement of Membrane-Bound Protein Tyrosine Kinases in the Zebrafish Egg
Establishing Relationships Between Closely Related Species Using Total Genomic DNA as a Probe
Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp T
Mutagenesis in Arabidopsis
剪接体(spliceosome)
Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression
Working Cell Bank
Covalent Immobilization of Enzymes to Graphitic Particles
Manipulating and Imaging the Early Xenopus laevis Embryo
Derivation and Culture of Canine Embryonic Stem Cells
Direct Sequencing of Differential Display PCR Products
Two-Hybrid Analysis of RasRaf Interactions
Special Stains for Extracellular Matrix
Direct MALDI-TOF Mass Spectrometric Peptide Profiling of Neuroendocrine Tissue of Drosophila
Manipulation of Gene Expression During Zebrafish Embryonic Development Using Transient Approaches
Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells
Restriction Enzymes
Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins
Techniques for the Study of Apoptosis in Bone
Measurement of Low-Frequency DNA Breaks Using Nucleoid Flow Cytometry
Assessing the Involvement of Telomerase in Stem Cell Biology
Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration
Identification and Characterization of Small GTPase-Associated Kinases
Establishing Cell-Based Reporter Systems for the Analysis of hTERT Expression
Raf/MEK/MAPK Signaling Stimulates the Nuclear Translocation and Transactivating Activity of FOXM1
Immobilization of Enzymes on Inorganic Supports by Covalent Methods
Differentiating Embryonic Stem Cells into Embryoid Bodies
Techniques in Protein Methylation
Xenopus tropicalis Oocytes: More Than Just a Beautiful Genome
Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in th
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
磷脂(phospholipids)
Eukaryote Growth Dynamics
植物遗传转化中基因受体的选择
吞噬作用(phagocytosis)
Isolation and Culture of Rhesus Adipose-Derived Stem Cells
Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias latipes
A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptor
肌动蛋纤维去聚合蛋白(actin filament-depolymerizing protein)
Treatment of Tissue Sections for In Situ Hybridization
Imaging Drosophila Pupal Wing Morphogenesis
Analyses of Gene Function in Amphioxus Embryos by Microinjection of mRNAs and Morpholino Oligonucleo
Lipidomics of the Red Cell in Diagnosis of Human Disorders
Nucleocytoplasmic Glycosylation, O-GlcNAc: Identification and Site Mapping
Protocols for Nuclear Transfer in Mice
Hypoxia-Inducible Factors: Post-translational Crosstalk of Signaling Pathways
Female Moth Pheromones
Studying Recombination in Mouse Oocytes
Genetic Mapping of Anti-Apoptosis Pathways in Myeloid Progenitor Cells
Isolation of Actin and Myosin Filaments
Study of Cell Cycle Checkpoints Using Xenopus Cell-Free Extracts
In Vivo Skin-Targeted Gene Delivery by Pulsed Electric Fields
Constitutively Active STAT5 Constructs
Efficient Generation of Dopamine Neurons from Human Embryonic Stem Cells
Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs
Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by
Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Resp
Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations
A High-Throughput Mammalian Cell-Based Transient Transfection Assay
锚定蛋白(ankyrin)
Stepwise Differentiation from Nave State Pluripotent Stem Cells to Functional Primordial Germ Cells
An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells
Vertical Arrays: Microarrays of Complex Mixtures of Nucleic Acids
Fusion Protocol for the Production of Mouse Hybridomas
Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells
An Assay for Human Chemosignals
Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells
Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells
质体(plastid)
Cdc34 蛋白（Cdc34 protein）
Analysis of Mammary Gland Morphogenesis
Immunohistochemical Analysis of mTOR Activity in Tissues
The Animal Cap Assay
Genetic Manipulation of Human Embryonic Stem Cells by Transfection
Micro-CT Technique for Three-Dimensional Visualization of Human Stem Cells
Detection of Gene Expression in Mouse Embryos and Tissue Sections
The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids
Immunostaining of Germline Stem Cells and the Niche in Drosophila Ovaries
Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection
美国JRH公司EX-CELLTM 302无血清培养基配制方法
In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections
Stabilization of Immobilized Enzymes by Chemical Modification with Polyfunctional Macromolecules
Experimental Manipulation and Morphometric Analysis of Neural Tube Development
Quail-Chick Chimeras
酵母遗传学方法2：蛋白质的抽提
数量性状实验
转运蛋白(translocator)
Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Re
Measurement of Phospholipase C Activity in Brain Membranes
PET-Radioimmunodetection of Integrins: Imaging Acute Colitis Using a 64Cu-Labeled Anti-7 Integrin An
阻断FC受体方法
Analyzing CellCell Interactions in 3-Dimensional Adhesion Assays
Human Mesenchymal Stem Cell Culture for Neural Transplantation
局部介质(local mediators)
Mammalian cells are amenable to study the regulation of cell cycle progression in vitro by shifting
Synchronization of Mammalian Cell Cultures by Serum Deprivation
Histological Approaches to Study Tissue Parasitism During the Experimental Trypanosoma cruzi Infecti
Cell-free System for the examination of apoptotic activity in cytoplasmic extracts
Analysis of DNA Topology in Yeast Chromatin
Quantitation of 25-OH-Vitamin D (25OHD) Using Liquid Tandem Mass Spectrometry (LC-MS-MS)
Human Prostate Epithelial Cell Cultures
Monitoring Removal of Cyclobutane Pyrimidine Dimers in Arabidopsis
Flow Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products
Detection of Plasmid by Colony Hybridization
In Situ Hybridization with Radiolabeled cRNA Probes, Using Tissue Sections and Smears
Assessment of Embryotoxicity Using Mouse Embryo Culture
Analysis of CTG Repeats Using DM1 Model Mice
Mitochondrial genome integrity is an important issue in somatic mitochondrial genetics. Development
Collection of Isolated Cells for Studying Mitochondrial DNA Mutations Within Individual Cells
通讯连接(communicating junction)
Motility Signaled From the EGF Receptor and Related Systems
Transgenic Reporter Tools Tracing Endogenous Canonical Wnt Signaling in Xenopus
DNA Ligation and Escherichia coli Transformation
支原体污染的特点及检验的笔记
原代细胞冻存实验操作步骤
Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Re
Production of Avian Chimeras and Germline Transmission
Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System
Microinjection of Antisense Oligonucleotides and Electrophysiological Recording of Whole-Cell Curren
Isolation and Grafting of Single Muscle Fibres
Isolation and Cultivation of Dermal Stem Cells that Differentiate into Functional Epidermal Melanocy
Generation of Multipotent CD34+CD45+ Hematopoietic Progenitors from Human Induced Pluripotent Stem C
Embryonic Stem Cell Derivation from Human Embryos
Cytogenetic Techniques for Human Leukemias
Peptide Recognition Mechanisms of Eukaryotic Signaling Modules
Rat x Rat Hybridomas
Generation of iPS Cells from Human Skin Biopsy
Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movem
Using Small Molecules to Improve Generation of Induced Pluripotent Stem Cells from Somatic Cells
Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration
中国首次研制出流感嗜血杆菌选择性培养基
生物安全柜与通风柜、超净工作台的区别
细胞电融合实验
细胞培养最常见注意事项
Nuclear Gene Defects in Mitochondrial Disorders
Fluorescence-Activated Cell Sorting According to Receptor Density: Application for Isolating Transfe
Xenotransplantation of Embryonic Stem Cell-Derived Motor Neurons into the Developing Chick Spinal Co
Retroviral Delivery of ECM Genes to Cells
Identification of Transplanted Human Cells in Animal Tissues
Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins
Assessing Tumor Angiogenesis in Histological Samples
偶线期(zygotene stage)
Use of Liposomes to Study Vesicular Transport
Claudin-5 Expression in In Vitro Models of the BloodBrain Barrier
Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Developme
Isolation and Characterization of Stem Cell-Enriched Human and Canine Hair Follicle Keratinocytes
Methods to Induce Cell Cycle Checkpoints
Monoclonal Antibodies in the Analysis of Embryonic Development
质体醌(plastoquinone, PQ)
ES Cell Culture and Manipulation
单克隆抗体细胞融合实验操作步骤及注意事项
Adsorbed Cationic Polymer Coatings for Enhanced Capillary Electrophoresis/Mass Spectrometry of Prote
SAPK/Jun kinase assays
联会复合体（synaptonemal complex,SC）
Targeted Mutagenesis Mediated by the Triple Helix Formation
Molecular Profiling of Hematopoietic Stem Cells
Determination of Functional Viral Titer by Drug-Resistance Colony Assay, Expression of Green Fluores
原核细胞(prokaryotic cell)
大鼠肌钙蛋白T(Tn-T)酶联免疫分析（ELISA）
细胞分裂的形态观察
Method: Lymphocyte Transformation
T-Cell Activation Using mAb to CD3
Gain-of-Function and Loss-of-Function Strategies in Xenopus
Arabidopsis Mitochondrial Proteomics
原代神经细胞培养实验方法
Linear RNA Amplification for the Production of Microarray Hybridization Probes
Abnbio dpbs缓冲液 A141905 不含氯化钙、氯化镁和酚红 500ml
Abnbio DMEM高糖培养基,High Glucose（无丙酮酸钠） A119655 500ml
Abnbio M199培养基 EBSS*含L-谷氨酰胺 L-氨基酸 A111505 500ml
Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration, and Tumorigenesis
Abnbio MEM培养基含L- 谷氨酰胺 A110955 500ml
Protein Extraction From Mammalian Tissues
Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors
Oxidative Stress and Plant Mitochondria
植物组织细胞培养
细胞生长检测9：染料染色法测定细胞数
Mass Spectrometry Analysis of Polyisoprenoids Alcohols and Carotenoids via ESI(Li+)-MS/MS
Preparation of Rat Liver Cell Cytosol
Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation
Determination of Tetrahydrozoline in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-
原生质理论（protoplasm theory）
Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protoco
Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in
Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differentiation Factors
Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell Plates
Isolation and Purification of Functionally Intact Mitochondria from Plant Cells
S期促进因子(S phase-promoting factor,SPF)
PEG介导的动物细胞融合技术
细胞分泌(cell secretion)
Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics
Dorsal Axis Duplication as a Functional Readout for Wnt Activity
High-Throughput FACS Method for Directed Evolution of Substrate Specificity
Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemi
Fate of Desmosomal Proteins in Apoptotic Epidermal Cells
ISH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results
Detection of Ubiquitination in 2DE
Redox-Sensitive Green Fluorescent Protein: Probes for Dynamic Intracellular Redox Responses. A Revie
Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection
In Vivo SELEX in Vertebrate Cells
Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedur
In Vitro Maturation (IVM) of Porcine Oocytes
Nuclear Transfer in Cattle
Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins
推荐一种简单实用的细胞冻存方法
Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes
In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tub
Practical Approaches for Implementing Forward Genetic Strategies in Zebrafish
Detection and Measurement of Reactive Oxygen Intermediates in Mitochondria and Cells
Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells
Strategies to Isolate Evolutionarily Conserved Cell Cycle Regulatory Genes
Restriction Site Mapping
人可溶性细胞间粘附分子1(sICAM-1)酶联免疫分析（ELISA）
膜结合核糖体(membrane-bound ribosomes)
Studying Osteocyte Function Using the Cell Lines MLO-Y4 and MLO-A5
动物细胞融合实验
Detection of Apoptosis in Tissue Sections
Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using th
Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy
Adipose-Derived Stem Cells for Skin Regeneration
Tat-Mediated Delivery of Antibodies into Cultured Cells
High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout
Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells
Site-Directed Mutagenesis Using a Rapid PCR-Based Method
Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary
Tips and Tricks for mFISH
Purification and Characterization of Poly(ADP-Ribosyl)ated DNA Replication/Repair Complexes
FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast
High-Resolution NMR of DNA and Drug-DNA Interactions
Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells
Percent Labeled Mitosis Curve Analysis
Electroporation-Mediated Gene Transfer into Hepatocytes
Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids
细胞生物基本方法：神经组织细胞培养
Antisense-Mediated Inhibition of Protein Synthesis: Rational Drug Design, Pharmacokinetics, Intracer
Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium
Protein Extraction for 2DE
Freezer and Cell Line Database
Assay of Phospholipase D Activity in Cell-Free Systems
Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of Mesenchymal Chondrogenesis
Flow Cytometry and Immunoselection of Human Stem Cells
Reprogramming of B Cells
Harvesting Hematopoietic Cells from Mice
Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model
Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cel
Staining Methods for cell death
培养基制备的基础实验
Generation of a Pool of Human Platelet Lysate and Efficient Use in Cell Culture
N-连接糖基化(N-linked glycosylation)
单链断裂重组模型（model of single-strand breaks recombination）
Gel Electrophoresis-Based Proteomics of Senescent Tissues
N-连接糖基化(N-linked glycosylation)
单链断裂重组模型（model of single-strand breaks recombination）
Gel Electrophoresis-Based Proteomics of Senescent Tissues
RAP-PCR Using RNA from Tissue Microdissection
Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1)
Measurement of Activities of Cyclobutane-Pyrimidine-Dimer and (6-4)-Photoproduct Photolyases
体外细胞培养对细胞培养基几点要求
EB病毒诱导淋巴细胞永生化实验方法
Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for th
Ultrasound-Responsive Liposomes
Slug Phototaxis, Thermotaxis, and Spontaneous Turning Behavior
In Situ Localization of Apoptosis Using TUNEL
Quantitative Analysis of miRNA Expression in Epithelial Cells and Tissues
Cell-Free Fetal DNA Plasma Extraction and Real-Time Polymerase Chain Reaction Quantification
Clinical Trials for Solid Tumors Using Electrochemotherapy
Neurotransmitter Receptor Analysis in Transgenic Mouse Models
Primary Culture and Live Imaging of Adult Neural Stem Cells and Their Progeny
Live Imaging, Identifying, and Tracking Single Cells in Complex Populations In Vivo and Ex Vivo
结缔组织类细胞培养
Freeze-Etch Electron Tomography for the Plasma Membrane Interface
Local Application of Bone Morphogenic Protein on Developing Chick Embryos Using Fibrous Glass Matrix
Assessment of Receptor Internalization and Recycling
自我剪接(self-splicing)
Nonradioactive Northern Blotting
Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting
小鼠5羟色胺(5-HT)酶联免疫分析（ELISA）
Quantification of Poly(ADP-Ribose) In Vitro: Determination of the ADP-Ribose Chain Length and Branch
Microexplant Cultures of the Cerebellum
Microexplant Cultures of the Cerebellum
Computer-Assisted Systems for the Analysis of Amoeboid Cell Motility
Culturing and Differentiating Human Mesenchymal Stem Cells for Biocompatible Scaffolds in Regenerati
Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration
Time-Lapse Fluorescence Microscopy of Saccharomyces cerevisiae in Meiosis
Binding Affinity and Kinetic Analysis of Nuclear Receptor/Co-Regulator Interactions Using Surface Pl
Clot Retraction
Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases
Adipogenic Differentiation of Human Induced Pluripotent Stem Cells
Frster Resonance Energy Transfer-Based Imaging at the Cell Surface of Live Cells
Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay
The Isolation of Membranes from Bacteria
G带(Giemsa-banding)
Simultaneous Real-Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
Monitoring Spindle Assembly and Disassembly in Yeast by Indirect Immunofluorescence
Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells
Transplantation of Embryonic Stem Cell-Derived Dopaminergic Neurons in MPTP-Treated Monkeys
Immunoblotting 2DE Membranes
Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease
同源异型基因（homeotic gene）
Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissue
Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors
Continuous-Flow Electrophoresis: Application to the Isolation of Lysosomes and Endosomes
Cos-1细胞暂时转染protocol
Protocol to Enrich and Analyze Plasma Membrane Proteins
Immunohistochemical Detection of Tumour Hypoxia
Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic
Preservation Protocols for Human Adipose Tissue-Derived Adult Stem Cells
Assessing Aging and Senescent Decline in Caenorhabditis elegans: Cohort Survival Analysis
Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas
Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency
Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes
Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics
Myoglobin Analysis
A Review of the Methods for Human iPSC Derivation
抗氧剂BHT知识百科
Methods for the Expression, Purification, Preparation, and Biophysical Characterization of Construct
人血管内皮细胞粘附分子1 (VCAM-1)酶联免疫分析（ELISA）
Plasma Polymer and PEG-Based Coatings for DNA, Protein and Cell Microarrays
Array CGH: Opening New Horizons
A pH-Indicator-Based Screen for Hydrolytic Haloalkane Dehalogenase
Microisolation and Microanalysis of Plasmid DNA
Culture of Human Embryonic Stem Cells (hESC)
Purification and Depletion of RNP Particles by Antisense Affinity Chromatography
Site-Directed Mutagenesis In Vitro by Megaprimer PCR
Models for Gain-of-Function and Loss-of-Function of MMPs: Transgenic and Gene Targeted Mice
Prenatal Diagnostics on Uncultured Amniocytes
Methods for Creating Mutations in C. elegans That Extend Lifespan
Mouse Models of Viral Infection: Influenza Infection in the Lung
Freezing and Thawing cells
Adult Cell Fate Reprogramming: Converting Liver to Pancreas
Molecular Modeling and Molecular Dynamics Simulations of Membrane Transporter Proteins
Oral and Pharyngeal Epithelial Keratinocyte Culture
Analysis of Change
Immunoglobulin Class Switching: In Vitro Induction and Analysis
Gene Delivery to Mesenchymal Stem Cells
Preparation of Template DNA and Labeling Techniques
细胞遗传学(cytogenetics)
胆固醇(cholesterol)
Monitoring Nutrient Signaling Through the Longevity Protein p66SHC1
Regeneration of Skin and Cornea by Tissue Engineering
Measurement of Changes in Intracellular Calcium During Apoptosis
Monitoring the Cycling Activity of Cultured Human Keratinocytes Using a CFSE-Based Dye Tracking Appr
The Evolution of Mass Spectrometry in the Clinical Laboratory
大鼠血小板活化因子（PAF）酶联免疫分析（ELISA）
Activation of SAPK/JNKs In Vitro
One-Tube RT-PCR with Sequence-Specific Primers
The GAL4 System: A Versatile System for the Expression of Genes
The Corneal Pocket Assay
Preparation of Efficient Splicing Extracts From Whole Cells, Nuclei, and Cytoplasmic Fractions
Choosing an Adipose Tissue Depot for Sampling: Factors in Selection and Depot Specificity
Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick Translation
Adipose-Derived Stem Cells
Strategies for Cloning New MMPs and TIMPs
Infection with retroviruses
Imaging Calcium Sparks in Cardiac Myocytes
Introduction to Nuclear Magnetic Resonance
Nestin-Driven Green Fluorescent Protein as an Imaging Marker for Nascent Blood Vessels in Mouse Mode
Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases
Quantification of Lysophosphatidylcholine Species by High-Throughput Electrospray Ionization Tandem
Mechanisms of Neurulation
Isolation and Characterization of the Heat Shock RNA 1
Pre-embedding Electron Microscopy Methods for Glycan Localization in Chemically Fixed Mammalian Tiss
3D Cell Culture: A Review of Current Approaches and Techniques
Genetic Fate-Mapping for Studying Adult Cardiomyocyte Replenishment After Myocardial Injury
Chromatin Immunoprecipitation
Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements During Xenopu
细胞移动(cell locomotion)
Cell Motility and Invasion Assays
3D Structuring of Biocompatible and Biodegradable Polymers Via Stereolithography
Islet-Derived Progenitors as a Source of In Vitro Islet Regeneration
Mesoderm Induction in Xenopus: Oocyte Expression System and Animal Cap Assay
Assays for Vertebrate Sperm Chemotaxis
Isolation and Culture of Human Hepatocytes
内膜(inner membrane)
Detection of Mycoplasma Contaminations
Abnbio IMDM培养基含L-谷氨酰胺 HEPES 不含α-硫代甘油 A124405 500ml
Xenogeneic Lung Transplantation Models
Atomic Force Microscopy Measurements of Intermolecular Binding Forces
Parental Origin Determination FISH: Pod-FISH
E位点(exit site, E site)
Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multipa
Transmembrane Signaling by Receptor Oligomerization
Isolation and Culture of Bovine Embryonic Stem Cells
Isolation of Cytokine-Inducible Genes from Hematopoietic Cells by Differential Display
胞间连丝(plasmodesmata)
Therapeutic Uses of Antioxidant Liposomes
Analysis of Changes in the Expression Pattern of Claudins Using Salivary Acinar Cells in Primary Cul
Carboxyfluorescein as a Marker at Both Light and Electron Microscope Levels to Follow Cell Lineage i
Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Si
Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay
光反应中心复合物(reaction-center complex)
DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules
Direct Labeling of Components in Protein Complexes by Immuno-Electron Microscopy
Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry
Defining Hormone and Matrix Requirements for Differentiated Epithelia
An Integrative Procedure for Apoptosis Identification and Measurement
DNA Methylation Analysis of Germ Cells by Using Bisulfite-Based Sequencing Methods
In Vivo Mechanical Loading
有丝分裂器(mitotic apparatus)
Ultrastructural Analysis of Testicular Tissue and Sperm by Transmission and Scanning Electron Micros
Assaying In Vitro Refolding of RNases by Mass Spectrometry
细胞通讯(cell communication)
G418筛选稳定表达细胞系
Statistical Analysis of Biological Rhythm Data
Isolation of c-Kit+ Human Amniotic Fluid Stem Cells from Second Trimester
Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Ch
Detection of DNA Strand Breakage in the Analysis of Apoptosis and Cell Proliferation by Flow and Las
Approaches to Separate and Identify PolyADP-Ribosylated Proteins Using Poly(ADP-Ribose) Glycohydrola
Ras and Rac as Activators of Reactive Oxygen Species (ROS)
Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation
Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR
Fertilization In Vitro
Stable Integration of Large PAC Constructs in Keratinocytes
Northwestern Screening of Expression Libraries
Analysis of Cell Migration Using Drosophila as a Model System
Sequence Analyses to Study the Evolutionary History and Cis-Regulatory Elements of Hedgehog Genes
Assessing Protein Synthesis by Platelets
Measuring Permeability in Human Retinal Epithelial Cells (ARPE-19): Implications for the Study of Di
Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells
Studying Wnt Signaling in Xenopus
Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and Their Applicat
3H-Thymidine Uptake by Cultured Cells
Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells
着丝点
欧氏平衡盐(粉末型)成分
Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ In Vitro
Chondrogenic Differentiation of hESC in Micromass Culture
Use of I-Sce I to Induce DNA Double-Strand Breaks in Nicotiana
变态(metamorphosis)
Assays for Detection of RNase A Superfamily Ribonucleases
Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells
鬼笔环肽(phalloidin)
RNAi Knockdown of Redox Signaling Protein Ape1 in the Differentiation of Mouse Embryonic Stem Cells
Chromatin Immunoprecipitation Assay for Mammalian Tissues
Consequences of Stress in the Secretary Pathway: The ER Stress Response and Its Role in the Metaboli
你知道基础培养基的成分么？
Cultivation of Keratinocytes from the Outer Root Sheath of Human Hair Follicles
Progress Towards Drosophila Epithelial Cell Culture
Vitrifying and Warming of Human Oocytes, Embryos, and Blastocysts: Vitrification Procedures as an Al
Assessing G1-to-S-Phase Progression After Genotoxic Stress
Bcl-2蛋白(Bcl-2 protein)
Hematopoietic Stem Cell Mobilization with Agents Other than G-CSF
Biosynthesis of Proteins Inside Liposomes
二联管(doublet)
Proteomics of Yeast Mitochondria
Single-Cell Calcium Imaging
Tandem Affinity Purification and Identification of Heterotrimeric G Protein-Associated Proteins
The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients
Quantifying In Vivo Phosphoinositide Turnover in Chemotactically Competent Dictyostelium Cells
A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Seque
In Vitro Studies on Odontogenic Tumors
Paraoxonases (PON1, PON2, PON3) Analyses In Vitro and In Vivo in Relation to Cardiovascular Diseases
WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells
Serum Protein Electrophoresis
Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans
Quantification of Cells in Culture
Noncovalent pADPr Interaction with Proteins and Competition with RNA for Binding to Proteins
Generation of Induced Pluripotent Stem Cells from Human Amnion Cells
Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U
Photoactivatable-GFP--Tubulin as a Tool to Study Microtubule Plus-End Turnover in Living Human Cells
细胞生物学(cell biology)
流式细胞仪检测外周血树突状细胞
小鼠海马神经元培养
Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System
Whole Embryo Imaging of Hematopoietic Cell Emergence and Migration
Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes
Methods for Double Detection of Gene Expression: Combined In Situ Hybridization and Immunocytochemis
Caenorhabditis elegans as a Model System for Triplet Repeat Diseases
Cell Sheet Technology for Tissue Engineering: The Self-Assembly Approach Using Adipose-Derived Strom
Mammalian Preimplantation Embryo Culture
Conjunctiva Organ and Cell Culture
Serial Transplantation of Bone Marrow to Test Self-renewal Capacity of Hematopoietic Stem Cells In V
A First Approach for the Production of Human Adipose Tissue-Derived Stromal Cells for Therapeutic Us
受体交叉(receptor crossover)
A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays
细胞决定(cell determination)
Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members
Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Funct
Assaying Protein Kinase Activity
What Role Does Mitochondrial Stress Play in Neurodegenerative Diseases
Mouse Chimeras and the Analysis of Development
Ethical and Regulatory Issues for Clinical Trials in Xenotransplantation
Analysis of Germline Chromatin Silencing by Double-Stranded RNA-Mediated Interference (RNAi) in Caen
Bisulfite
Preparation of Human Trophoblast Cells for Culture
稳定转染攻略：从G418浓度确定到单克隆化鉴定
Organ Culture of Developing Mouse Skin and Its Application for Molecular Mechanistic Studies of Morp
细胞培养实验用品
Preparation of Mammalian Oocytes for Transmission Electron Microscopy
Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays
Polyamine Block of Inwardly Rectifying Potassium Channels
Sage: The Serial Analysis of Gene Expression
X-Ray Crystallography of Agonist/Antagonist-Bound Receptors
The Development of T Lymphocytes in Fetal Thymus Organ Culture
Exometabolomic Mapping of Caenorhabditis elegans: A Tool to Noninvasively Investigate Aging
Isolation, Cultivation, and Application of Human Alveolar Epithelial Cells
Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Mo
The Nuclear UbiquitinProteasome System: Visualization of Proteasomes, Protein Aggregates, and Proteo
Human Osteoclast Culture From Peripheral Blood Monocytes: Phenotypic Characterization and Quantitati
Capillary Electrophoresis of Sugar Acids
大鼠环磷酸腺苷(cAMP)酶联免疫分析
细胞质膜系统(cytoplasmic membrane system)
Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation
Primary Cultures of Rat Hepatocytes
Isolation of Cutaneous Dendritic Cells
Bioassays of Inductive Interactions in Amphibian Development
Evaluation of Antifungal Susceptibility Using Flow Cytometry
How to Measure Microtubule Dynamics
In Vitro Maturation and Fertilization of Canine Oocytes
Measuring Reactive Oxygen Species in Senescent Cells
Preclinical Testing of Virotherapeutics for Primary and Secondary Tumors of the Liver
Optimized Fixation and Immunofluorescence Staining Methods for Dictyostelium Cells
Zebrafish Immunohistochemistry
Differentiate ES cells into cardiac myocytes
Quantitation of Fentanyl in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)
Combined Bidimensional Electrophoresis and Electron Microscopy to Study Specific Plasmid DNA Replica
Derivation and Characterization of Hepatocytes From Embryonic Stem Cells In Vitro
Intravenous Human Mesenchymal Stem Cells Transplantation in NOD/SCID Mice Preserve Liver Integrity o
Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging
Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells
Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes
Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases
Quantitation of Indomethacin in Serum and Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS)
In Situ Hybridization to Polytene Chromosomes
被膜小窝(clathrin-coated pit)
Differential Screening for the Isolation of Species-Specific Sequences in the Anopheles farauti Comp
精原细胞（spermatogonia）
Practical Considerations for the Analysis of Ionic and Neutral Organic Molecules With Capillary Elec
An Efficient Way of Studying ProteinProtein Interactions Involving HIF-, c-Myc, and Sp1
Neural Crest Migration Methods in the Chicken Embryo
关于细胞培养中的污染
Hepatocyte Xenotransplantation
Quantitative In Situ Hybridization of Tissue Microarrays
Histochemical Localization of Cell Proliferation Using In Situ Hybridization for Histone mRNA
Quantitation of Ibuprofen in Blood Using Gas Chromatography-Mass Spectrometry (GC-MS)
Endothelial Cell Systems - Instructions For Use
红细胞膜的制备及其成份分析(磷脂、胆固醇)
Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentration
NF-Ya Protein Delivery as a Tool for Hematopoietic Progenitor Cell Expansion
Transplantation of Neural Stem/Progenitor Cells into Developing and Adult CNS
In Vivo Quantification of Metastatic Tumor Cell Adhesion in the Pulmonary Microvasculature
Studying Colloidal Aggregation Using Liposomes
Measurement of Mitochondrial Oxygen Consumption Using a Clark Electrode
Cytological Techniques to Study Human Female Meiotic Prophase
Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells
Immunoelectron Microscopy of Cryofixed Freeze-Substituted Saccharomyces cerevisiae
Mechanistic Studies of Skin Electroporation Using Biophysical Methods
Methods to Study Protein-Protein Interactions
Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones
Strategies Employed for the Development of PARP Inhibitors
果胶(pectin)
Yeast Two-Hybrid Screening
High Activity Preparations of Lipases and Proteases for Catalysis in Low Water Containing Organic So
$SDS Gel Electrophoresis of Tubulin\MAPs$
SDS Gel Electrophoresis of Tubulin\MAPs
Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods
Active Protease Mapping in 2DE Gels
Quantitation of Acute Necrosis After Experimental Myocardial Infarction
人胃动素(MTL)酶联免疫分析（ELISA）
Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors
The Chimeric Human/Mouse Model of Angiogenesis
Analysis of Microtubule-Mediated Intracellular Viral Transport
Nonradioactive In Situ Hybridization on Frozen Sections and Whole Mounts
Real-Time Visualization and Quantification of Native Ras Activation in Single Living Cells
Neuroepithelial Differentiation Induced by ECM Molecules
Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules
Analysis of the Degradome with the CLIP-CHIP Microarray
Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma
Genome Mapping Statistics and Bioinformatics
Detection of Intracellular/lntranuclear Antigens: Applications in Leukemia/Lymphoma Analysis
Disc Angiogenesis Assay
Circulating Endothelial Progenitor Cells (CEPC)
人肝细胞生长因子受体（HGFR/ c-MET）酶联免疫分析（ELISA）
冰冻蚀刻(freeze-etching)技术
遗传信息表达结构系统(genetic expression system)
细胞培养用血清保存方法与血清解冻常见问题
Preparation of NeoR Murine Embryonic Fibroblasts
从单个核细胞中分离出T细胞
稳定转染之后细胞荧光强度差异很大
确认过眼神，遇见「物美价廉的」示踪细胞株
Immunofluorescent Staining of Mouse and Rat Leukocytes
免疫荧光样品制备与观察
Detection In Vitro and Quantitative Estimation of Artificial Microterritories Which Promote Osteogen
DelsGate: A Robust and Rapid Method for Gene Deletion
Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells
The CGG Repeat and the FMR1 Gene
Antisense Therapy in Clinical Oncology: Preclinical and Clinical Experiences
Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Hum
Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled
Mutant Generation in Vertebrate Model Organisms by TILLING
Labeling Primitive Myeloid Progenitor Cells in Xenopus
Proliferation Assay: [3H] Thymidine incorporation
Microbial Molecular Epidemiology: An Overview
Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays
Collection, Processing, and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regen
Analysis of DNA Damage and Repair by Comet Fluorescence In Situ Hybridization (Comet-FISH)
Enrichment of Oral Mucosa and Skin Keratinocyte Progenitor/Stem Cells
Human Fetal Brain Cell Culture
Culturing Primitive Hemopoietic Cells: Long-Term Mouse Marrow Cultures and the Establishment of Fact
Immunoassay Measurements of Leptin
Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA)
Methods for Studying Vinca Alkaloid Interactions With Tubulin
Palatal Dysmorphogenesis: Palate Organ Culture
Generating Neurons from Stem Cells
Cardiac Stem Cell Niche, MMP9, and Culture and Differentiation of Embryonic Stem Cells
Induction of Intracellular Signals Through Binding of Adhesive Molecules: Activation of p125FAK Tyro
Whole-Mount Imaging of Responses in Mouse Vomeronasal Neurons
Poly(Carbamoyl Sulfonate) Hydrogels
Activity of MAPK/p90rsk During Fertilization in Mice, Rats, and Pigs
Bovine Retinal Microvascular Pericytes: Isolation, Propagation, and Identification
Saccharomyces cerevisiae as a Model Organism to Study Mitochondrial Biology: General Considerations
Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers
General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds
Embryo/Fetal Topographical Analysis by Fluorescence Microscopy and Confocal Laser Scanning Microscop
Enzymatic Isolation and Serum-Free Culture of Human Renal Cells: Retaining Properties of Proximal Tu
Examining Planar Cell Polarity in the Mammalian Cochlea
Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized L
Assay of Deoxyhypusine Hydroxylase Activity
Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes
Integration of Protein Kinases into Transcription Complexes: Identifying Components of Immobilised I
The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair
Determination of the Activity of Rho-Like GTPases in Cells
Methods to Assess Tissue Permeability
自体组装(self assembly)
核糖体
Nucleotide Metabolism and Cell-Cell Interactions
FISH on Sperms, Spermatocytes and Oocytes
细胞转染方法
Tissue Culture Media
减数分裂
QUALITATIVE ANALYSIS OF DNA FRAGMENTATION BY AGAROSE GEL ELECTROPHORESIS
Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry
Isolation of Murine Adipose-Derived Stem Cells
Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes
Preparation of Isolated Periportal or Perivenous Hepatocytes from Rat Liver
Isolation and Growth of Adipose Tissue-Derived Stem Cells
In Vitro Clonal Culture of Human Hematopoietic Progenitor Cells
Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis
High Throughput Analysis of Nuclear ReceptorCofactor Interactions
Mechanical Stimulation of Bone Cells Using Fluid Flow
高通量细胞筛选技术(high throughput cell-based screening technology)
The Immunodetection of pADPr in Drosophila Tissues: Immunostaining of Thick Sections; Immuno-EM
RNA-Protein Crosslinking with Photoreactive Nucleotide Analogs
Methods to Study Transcription-Coupled Repair in Chromatin
Preparation of Human Partial Chromosome Paints from Somatic Cell Hybrids
造血干细胞移植（HSCT）的适应症
Determination of Unknown Genomic Sequences Without Cloning
Drosophila as a Genetic Tool to Define Vertebrate Pathway Players
Microbodies
Serum-Free Culture of Mid-gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs
Methods for the Study of Redox-Mediated Changes in p53 Structure and Function
Rat Aortic Ring: 3D Model of Angiogenesis In Vitro
PCR产物直接转染细胞并转录siRNA说明
Characterizing ECM Production by Cells Encapsulated in Hydrogels
Studying Head and Brain Development in Drosophila
Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets
Troubleshooting Image Analysis in 2DE
Mouse Hematopoietic Stem Cell Transplantation
Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth
Spermidine/Spermine-N1-Acetyltransferase in Kidney Ischemia Reperfusion Injury
MAP Kinase Activation by Receptor Tyrosine Kinases: In Control of Cell Migration
Plant Protein Extraction
In Vitro Ubiquitination of Cytokine Signaling Components
In Vitro Maturation and Embryo Production in Cattle
Therapeutic Inhibition of Angiogenesis
Membrane Protein Expression in Lactococcus lactis
Assessment of Spermatogenesis Through Staging of Seminiferous Tubules
Fluorescence Recovery After Photobleaching (FRAP) to Study Nuclear Protein Dynamics in Living Cells
Maturation Changes in Purified Phagosomes
大鼠抗缪勒管激素(AMH)酶联免疫分析（ELISA）
Isolation Procedure and Characterization of Multipotent Adult Progenitor Cells from Rat Bone Marrow
High-Resolution Large-Gel 2DE
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secr
In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
The Application of Randomly Amplified DNA Analysis in the Molecular Epidemiology of Microorganisms
Isolation, Growth, and Characterization of Human Renal Epithelial Cells Using Traditional and 3D Met
Ubiquitination of G-Protein-Coupled Receptors
Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates
Analysis of Triplet Repeat Replication by Two-Dimensional Gel Electrophoresis
杀灭黑胶虫的一种方法
Released Chromatin or DNA Fiber Preparations for High-Resolution Fiber FISH
Data Analysis in Cell Kinetics Research
Retroviral Vector-Based Approaches for the Generation of Human Induced Pluripotent Stem Cells from F
Chromosome Sorting and Analysis by FACS
Identification of Genes Associated with Resistance to Photodynamic Therapy-Mediated Oxidative Stress
An In Vitro System for Measuring the Transdermal Voltage and Molecular Flux Across the Skin in Real
Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes
Biochemical Methods Used to Study the Gene Expression and Protein Complexes in the Filamentous Fungu
分子发动机(molecular motor)
Detecting Polymorphisms in MMP Genes
Expression of Epitope-Tagged Proteins in Plants
Cell Sorting of Young and Senescent Cells
Assaying Lipid Phosphate Phosphatase Activities
Analysis of PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays
Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time
Measurements of Active TGF Generated by Culture Cells
Membrane Translocation Assayed by Fluorescence Spectroscopy
单管(singlet)
Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells
Mammalian Fertilization Is Dependent on Multiple Membrane Fusion Events*
Modeling Brain Pathologies Using Neural Stem Cells
Direct Ex Vivo Activation of T cells for Analysis of Dendritic Cells Antigen Presentation
G Protein-Coupled Receptor Dimerization and Signaling
Pheromones in the Fruit Fly
Mobilization of Hematopoietic Stem Cells by Depleting Bone Marrow Macrophages
Separation of Labeled Inositol Phosphate Isomers by High-Pressure Liquid Chromatography (HPLC)
Immunocytochemical Analysis of Neuronal Differentiation
The Role of Hex in Hemangioblast and Hematopoietic Development
The Binding of Protein-Ligands to Cell-Surface Receptors
In Vivo Visualization of RNA Using the U1A-Based Tagged RNA System
Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mapping
Alginate Microbead Release Assay of Angiogenesis
Single-Cell Culture in Microwells
Bioinformatic Approaches to the Identification of Novel Neuropeptide Precursors
Quantitative Fluorescence Microscopy Techniques
Practical Aspects of OCT Imaging in Tissue Engineering
Automated Planar Patch-Clamp
Closed Ampoule Isothermal Microcalorimetry for Continuous Real-Time Detection and Evaluation of Cult
Mapping of Protein Transduction Pathways with Fluorescent Microscopy
Recombinant Adeno-Associated Viral Vector Production and Purification
大鼠半乳糖凝集素-9酶联免疫分析（ELISA）
Preparation of a Multi-antioxidant Formulation
信号转导(signal transduction)
Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In
Isolation and Culture of Primary Endothelial Cells
Zymography and Reverse Zymography for Detecting MMPs and TIMPs
The Mitochondria of Cultured Mammalian Cells: II: Expression and Visualization of Exogenous Proteins
Quantitation of Amobarbital, Butalbital, Pentobarbital, Phenobarbital, and Secobarbital in Urine, Se
Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells
Testicular Germ Cell Tumors in Mice: New Ways to Study a Genetically Complex Trait
In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells
Cloning of Cytokine 3 Untranslated Regions and Posttranscriptional Assessment Using Cell-Based GFP A
Reporter/Functional Gene Transfer in Rat Brain
Looking for the Niche: Substance Delivery into the Lateral Ventricle of the Brain: The Osmotic Minip
The Zebrafish: An Overview of Its Early Development
The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations
Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-Ray Crystallography
The Basic-Helix-Loop-Helix-Leucine Zipper Gene Mitf: Analysis of Alternative Promoter Choice and Spl
Histochemistry and Lipid Profiling Combine for Insights into Aging and Age-Related Maculopathy
Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase
Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Poly
Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins
单克隆抗体的制备与鉴定方法
Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
Glomerular Epithelial and Mesangial Cell Culture and Characterization
Matrix Assembly, Cell Polarization, and Cell Survival: Analysis of Peri-Implantation Development Wit
Isolation and Purification Method of Mouse Fetal Hepatoblasts
Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria
Assays of Lipolytic Enzymes
Preparing Mitochondria from Rat Liver
Isolation and Culture of Bone-Forming Cells (Osteoblasts) from Human Bone
Tracking Nanoparticles in Three-Dimensional Tissue-Engineered Models Using Confocal Laser Scanning M
分子细胞生物学(molecular biology of the cell)
细胞死亡(cell death)
ChIP on Chip Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications
兔白细胞介素6（IL-6）酶联免疫分析（ELISA）
Processing mRNA 3' Ends In Vitro
Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord
Mapping the Lipolytic Proteome of Adipose Tissue Using Fluorescent Suicide Inhibitors
Gene Targeting by Oligonucleotides in Keratinocytes
Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstal
Study of Skeletal Myogenesis in Cultures of Unsegmented Paraxial Mesoderm
Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)
Broad Spectrum Drug Screening Using Electron-Ionization Gas Chromatography-Mass Spectrometry (EI-GCM
Use of Escherichia coli for the Production and Purification of Membrane Proteins
Isolation of Mitochondria From Procyclic Trypanosoma brucei
Engineering Elements for Gene Silencing: The Artificial MicroRNAs Technology
The Stem Cell Laboratory: Design, Equipment, and Oversight
Direct Sequencing by Thermal Asymmetric PCR
Fluorescent Glycerolipid Probes: Synthesis and Use for Examining Intracellular Lipid Trafficking
Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR
Isolation of Sperm Nuclei and Nuclear Matrices from the Mouse, and Other Rodents
Monitoring of the Dynamics of Epithelial Dome Formation Using a Novel Culture Chamber for Long-Term
分子生物学的三部分研究内容
Numerical Modeling for In Vivo Electroporation
Profiling Axonal mRNA Transport
内膜系统(endomembrane systems)
大鼠白细胞介素2（IL-2）酶联免疫分析
Survival Analysis
Viability Cell Count
Dissecting Mesenchymal Stem Cell Movement: Migration Assays for Tracing and Deducing Cell Migration
Detection and Isolation of Trinucleotide Repeat Expansions Using the RED Method
In Vitro Selection of Allosteric Ribozymes
Keratocyte Cell Culture
Anti-claudin-4-Conjugated Highly Luminescent Nanoparticles as Biological Labels for Pancreatic Cance
Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells
The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluoresc
The Purification of Mouse Hematopoietic Stem Cells at Sequential Stages of Maturation
Electrochemical-Based Immobilization of Enzymes
Measuring Proteolysis in Mitosis
Immunizing Schedules for Hybridoma Production
Stem Cells for Myocardial Repair and Regeneration: Where Are We Today
Retroviral delivery of ECM genes
Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells
A Method to Visualize the Actin and Microtubule Cytoskeleton by Indirect Immunofluorescence
Measurement of Cell-Penetrating Peptide-Mediated Transduction of Adult Hematopoietic Stem Cells
Database Setup for Preclinical Studies of Gene-Modified Hematopoiesis
Suprachiasmatic Nucleus Cultures That Maintain Rhythmic Properties In Vitro
Immobilization of Enzymes on Microelectrodes Using Chemical Crosslinking
Selective Detection of Phagocytic Phase of Apoptosis in Fixed Tissue Sections
Determination of mRNA and Protein Expression Patterns in Zebrafish
Live-Cell Fluorescence Imaging of Meiotic Chromosome Dynamics in Schizosaccharomyces pombe
Ratiometric Ca2+ Measurements Using the FlexStation Scanning Fluorometer
Methods to Measure Membrane Potential and Permeability Transition in the Mitochondria During Apoptos
Experimental Identification of MicroRNA Targets by Immunoprecipitation of Argonaute Protein Complexe
Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryoni
Immobilization of Enzymes and Cells: Some Practical Considerations
Structural Analysis of Protein-RNA Interactions With Mass Spectrometry
关于动物血清的常见问题及处理方法
Chemical Assays for Proteins
Generation of Human-Induced Pluripotent Stem Cells (hiPSCs) Using Episomal Vectors on Defined Essent
Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a
Introduction to Nucleocytoplasmic Transport: Molecules and Mechanisms
Analysis of Biomarkers of Caloric Restriction in Aging Cells
常用封闭剂选择的窍门
Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and
Identification of Mutated p53 in Cancers by Nongel-Sieving Capillary Electrophoretic SSCP Analysis
Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture
矽肺(silicosis)
Activation of the Mitogen-Activated Protein Kinase Pathway by TGF
Detection of the Senescence-Associated Secretory Phenotype (SASP)
Stallion Spermatozoa Viability: Comparison of Flow Cytometry with Other Methods
Measurements of Stathmin-Tubulin Interaction in Solution
Biochemical Responses to Chemoattractants in Dictyostelium: Ligand-Receptor Interactions and Downstr
Analysis of Glycosaminoglycans in Stem Cell Glycomics
Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells
Antisense Oligonucleotide Treatment of Inflammatory Bowel Diseases
The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Prote
Quantitation of Cellular and Topical Uptake of LuciferinOligoarginine Conjugates
A Cellular Viability Assay to Monitor Drug Toxicity
The Extraction and Analysis of Glycosphingolipids
Mechanical Properties of Interphase Nuclei Probed by Cellular Strain Application
Studying the Function of Dendritic Cells in Mouse Models of Asthma
Transwell® 通透性支持物的选择和使用手册
The Sponge Implant Model of Angiogenesis
Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells
Antibodies to MMP-Cleaved Aggrecan
Separation of Platelets from Whole Blood
Assay of Cell Cycle Kinetics by Multivariate Flow Cytometry Using the Principle of Stathmokinesis
分子生物学(molecular biology)
Biomedical Applications of Amino Acid Detection by Capillary Electrophoresis
Acetonitrile Stacking: Serum Phenobarbital as an Example
Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning
Imaging Pluripotent Cell Migration in Drosophila
In Vivo and Ex Vivo Gene Transfer in Thymocytes and Thymocyte Precursors
FISH in Food Microbiology
Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models
Micelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase Assays
Peptidomic Approaches to the Identification and Characterization of Functional Peptides in Hydra
Computational Modeling of Signaling Networks for Eukaryotic Chemosensing
Characterization of DNA Condensates by Atomic Force Microscopy
HGF-Induced DU145 Cell Scatter Assay
Using Organotypic Tissue Slices as Substrata for the Culture of Dissociated Cells
Assessment of In Vitro and In Vivo Mitochondrial Function in Friedreich’s Ataxia and Huntington’s Di
Ultracentrifugation Technique for Measuring the Binding of Peptides and Proteins to Sucrose-Loaded P
Mass Measurement of Key Phosphoinositide Cycle Intermediates
Basic Protocols to Investigate hMSC Behavior onto Electrospun Fibers
Isolation of Sea Urchin Sperm Plasma Membranes
Human Thyroid Epithelial Cells
A High-Throughput Sequencing-Based Methodology to Identify All Uncapped and Cleaved RNA Molecules in
Flow Cytometry: Immunofluorescence Staining of Activated and Rest
Using TUNEL in Combination with an Active Caspase-3 Immunoassay to Identify Cells Undergoing Apoptos
Isolation of Human Blood DC Subtypes
Methods for the Analysis of the Sperm Proteome
Pig Neural Cells Derived from Foetal Mesencephalon as Cell Source for Intracerebral Xenotransplantat
Freezing Cells
Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches
The Chick Embryo as a Model System for Analyzing Mechanisms of Development
Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloprotei
Transcriptome Analysis of Drosophila Neural Stem Cells
Isolation of Total RNA from Tissues or Cell Lines: Visualization in Gel
Generation of Protease-Deficient Strains and Their Use in Heterologous Protein Expression
Flow Cytometric Detection of Fluorescent Redistributional Dyes for Measurement of Cell Transmembrane
Epidermal Permeability Barrier Measurement in Mammalian Skin
奥林巴斯倒置显微镜CKX-31操作和维护说明
Microsequencing of Myosins for PCR Primer Design
A Detailed Protocol for Bacterial Artificial Chromosome Recombineering to Study Essential Genes in S
Monitoring DNA Damage During Cell Senescence
Negative Stain Electron Microscopy of Microtubules
Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1
Utilizing DNA Mutations to Trace Epithelial Cell Lineages in Human Tissues
In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages
Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene
Production of Tissue-Engineered Skin and Oral Mucosa for Clinical and Experimental Use
细胞培养中几个错误认识
Monoclonal Antibodies Against Glycosphingolipids (GSLs)-Gangliosides
Primary Culture of Ovarian Cells for Research on Cell Interactions in the Hormonal Control of Steroi
Isolation and Establishment of Hair Follicle Dermal Papilla Cell Cultures
Assessing the Function of mTOR in Human Embryonic Stem Cells
Vitrification of Plant Cell Suspensions
Media Composition: Growth Factors
Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Heterodimerization
Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Chorion, Amniocytes, and Fibroblast
In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors
Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers
Bioinformatic Approaches to Metabolic Pathways Analysis
Use of Capillary Electrophoresis for Polysaccharide Studies and Applications
减数分裂(meiosis)
Immobilized Culture and Transfection Microarray of Non-adherent Cells
Use of Doppler Echocardiography to Monitor Embryonic Mouse Heart Function
Identification of Apoptosis Regulatory Genes Using Insertional Mutagenesis
选择合适的标记方法
Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing
Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays
Measuring Inflammatory Cell Migration in the Zebrafish
姊妹染色单体交换实验
Isolation of Adult Stem Cells and Their Differentiation to Schwann Cells
Gene Targeting in Cultured Human Cells
Magnetic Resonance Imaging and Tracking of Stem Cells
DNA Labeling In Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Fo
微管（microtube）
The Culture and Maintenance of Functional Retinal Pigment Epithelial Monolayers from Adult Human Eye
High-Throughput and Longitudinal Analysis of Aging and Senescent Decline in Caenorhabditis elegans
Therapeutic Applications of Cell-Penetrating Peptides
Using siRNA Knockdown in HaCaT Cells to Study Transcriptional Control of Epidermal Proliferation Pot
小牛血清和胎牛血清的区别
Measuring Transcription of Metalloproteinase Genes: Nuclear Run-Off Assay vs Analysis of hnRNA
Three-Color FISH for the Detection of Individual Radiosensitivity
美国科学家发现一种用处极大的细菌
Genetic Modification of Mesenchymal Stem Cells
Whole Mount Immunohistochemistry and In Situ Hybridization of Larval and Adult Zebrafish Dental Tiss
Analysis of Epithelial Barrier Integrity in Polarized Lung Epithelial Cells
Use of Gene Targeting to Study Recombination in Mammalian DNA Repair Mutants
In Vitro Repair Model of Focal Articular Cartilage Defects in Humans
Telomere Neurobiology
Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers
Aggregating Neural Cell Cultures
Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45 Ca2+ and Fluo-3
血清热灭活的问题
Culturing HEK 293 Cells
微生物污染的排除
The Use of Consensus PCR and Direct Sequence Analysis for the Identification of HPV
Hematopoietic Colony-Forming Cells
The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes: Transcrip
C4途径(C4 pathway)
T cell Activation Protocol
Confocal Laser Scanning Microscopy Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos
ELISPOT (Enzyme-linked ImmunoSPOT)
三羧酸循环(citric acid cycle)
细胞培养中的黑胶虫污染问题的处理
The Cell Environment (including types of culture medium)
杂交瘤细胞的制备方法
红细胞裂解的实验方法步骤
Craniofacial Development of Avian and Rodent Embryos
Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation S
去壁低渗法制备植物染色体标本实验
Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
In Situ Detection of EpsteinBarr Virus and Phenotype Determination of EBV-Infected Cells
The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression
Single-Cell Enzymatic Dissociation of hESC Lines OxF1OxF4 and Culture in Feeder-Free Conditions
Cytotoxicity Assays Protocol
粗面内质网(rough endoplasmic reticulum, RER)
Fluorescence Microscopy of Microtubules in Cultured Cells
Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Bl
Study of Respiratory Cytochromes in Liposomes
Primary Cell Culture of Human Enteric Neurons: Submucosal Plexus
A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplas
Belcher/Knight: Electrocompetent Cells
In Situ PCR Detection of HIV Expression in the Human Placenta
细胞组分和细胞器――细胞膜
In Vitro Reprogramming of Pancreatic Cells to Hepatocytes
High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis
Assays of Bypass Replication of Genotoxic Lesions in Mammalian Disease and Mutant Cell-Free Extracts
Defining Pre-mRNA cis Elements that Regulate Cell-Specific Splicing
DNA Microarrays to Analyze Gene Expression in Animals with Altered Transporter Expression
小鼠白细胞介素-35（IL-35）酶联免疫分析（ELISA）
电穿孔与电融合技术
293细胞的大规模培养的方法
Cellularity Measurements
Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenet
Transdermal Drug Delivery by Skin Electroporation in the Rat
Retroviral-Mediated Transduction and Clonal Integration Analysis of Human Hematopoietic Stem and Pro
Quantification of MicroRNAs, Splicing Isoforms, and Homologous mRNAs With the Invader Assay
动物细胞大规模培养用生物反应器简介
Functional Analysis of Nocturnin: A Circadian Clock-Regulated Gene Identified by Differential Displa
Immunohistochemistry of MMPs and TIMPs
Real-Time Analysis of Integrin-Dependent Transendothelial Migration and Integrin-Independent Interst
Three-Dimensional Human Tissue Models of Wounded Skin
Microscopic Computed Tomography-Based Virtual Histology of Embryos
Intracellular Staining Protocol
Transgenic Mouse Models of Craniofacial Disorders
Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice
High Proliferative Potential Colony Forming Cells
A Human Cell Extract-Based Assay for the Activation of ATM and ATR Checkpoint Kinases
Organ Cultures and Kidney-Capsule Grafting of Tooth Germs
The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells
Liposomes for Drug Delivery to Mitochondria
Demonstration of Mycoplasma Contamination in Cell Cultures by a Mycoplasma Group- Specific Polymeras
关卡(check point)
Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yea
Clonal Analysis in the Examination of Gene Function in Drosophila
Microencapsulation of Stem Cells to Study Cellular Interactions
Chemotaxis and Chemokinesis in 3D Macromolecular Matrices: Relevance to Angiogenesis
Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells
Real-Time Analysis of G Protein-Coupled Receptor Signaling in Live Cells
Measuring Apoptosis in Neural Stem Cells
Treatment of Rat Bladder Cancer With Electrochemotherapy In Vivo
The Post-embedding Method for Immunoelectron Microscopy of Mammalian Tissues: A Standardized Procedu
Tracking Inflammation-Induced Mobilization of Mesenchymal Stem Cells
Purification and Culture of Fetal Mouse Hepatoblasts that Are Precursors of Mature Hepatocytes and B
细胞融合(Cell fusion)实验
Development of Biological Tools to Study Claudins in the Male Reproductive Tract
In Vitro Selection of glmS Ribozymes
Activation of Rayon/Polyester Cloth for Protein Immobilization
Overview: Studying Integrins In Vivo
Immobilization of Proteins on Thionyl Chloride-Activated Controlled-Pore Glass
Quantification of Asymmetric Microtubule Nucleation at Subcellular Structures
bcl-2 原癌基因（bcl-2 protooncogene）
Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy
Adhesion to Matrix Proteins
选择蛋白(selectins)
Recording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration
Testing Smell When It Is Really Vital: Behavioral Assays of Social Odors in the Neonatal Mouse
Analysis of Endothelial Cell Migration Under Flow
Detection of c-Myc ProteinProtein Interactions and Phosphorylation Status by Immunoprecipitation
Culture of Human Endothelial Cells from Umbilical Veins
Genetic Analysis of Cystic Fibrosis Airway Epithelial Cells
Sphingolipids in Macroautophagy
A Genetic Model to Dissect the Role of Tsc-mTORC1 in Neuronal Cultures
Radionuclide-Peptide Nucleic Acid in Diagnosis and Treatment of Pancreatic Cancer
用尼龙毛法分离B细胞
Biophysical Methods to Probe Claudin-Mediated Adhesion at the Cellular and Molecular Level
Regulation of Transcription Factor Function by Targeted Protein Degradation: An Overview Focusing on
Expression of Recombinant MMP-28 in Mammalian Cells
Bioinformatic Identification of Plant Peptides
Epidermal Cell Analysis by RT-PCR
Assessing Mitochondrial Potential, Calcium, and Redox State in Isolated Mammalian Cells Using Confoc
Current Thoughts on the Therapeutic Potential of Stem Cell
Murine Nonvolatile Pheromones: Isolation of Exocrine-Gland Secreting Peptide 1
Determination of Hydroxyurea in Serum or Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS)
Generation of Induced Pluripotent Stem Cells with CytoTune, a Non-Integrating Sendai Virus
Studying Fertilization in Cell-Free Extracts: Focusing on Membrane/Lipid Raft Functions and Proteomi
Cryopreservation of Mammalian Embryos: Slow Cooling
Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cell
Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pe
Minimization of Proteins by Random Fragmentation and Selection
Cytological Analysis of Meiosis in Fixed Drosophila Ovaries
Isolation and Purification of Plant Nucleic Acids: Genomic and Chloroplast DNA
Hoechst 33342/PI双染色法
体内肝损伤模型（酒精）
293T细胞的一点培养心得
Adrenal chromaffin granule (chromaffin vesicle) preparation
人(ASD)酶联免疫分析
Lacrimal Experimental Protocol (4/4/94)
PCR of the Ribosomal DNA Intergenic Spacer Regions as a Method for Identifying Mosquitoes in the Ano
Enrichment of PBMCs with monocytes (The Science Advisory Board)
胚胎干细胞培养标准化操作规程
Production of Neurospheres from CNS Tissue
Isopycnic Centrifugation of DNA: Extraction and Fractionation of Melon Satellite DNA by Buoyant Dens
Immobilization of Enzymes on Ethynyl-Modified Electrodes via Click Chemistry
The Role of Redox in Signal Transduction
Overview of Missing Data Techniques
Maintenance of Chicken Embryonic Stem Cells In Vitro
Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes
Human Tracheal Gland Cells in Primary Culture
Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells
Culture Systems: Air Quality
Overview: Structural Biology of Integrins
Renal Gene Therapy
Analyzing Association of the Endoplasmic Reticulum with the Legionella pneumophilaContaining Vacuole
Enzyme Immobilization Using Chitosan-Xanthan Complexes
Isolation and Expansion of Mesenchymal Stem Cells/Multipotential Stromal Cells from Human Bone Marro
Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli
Histone Deacetylase Activity Assay
Genetic Manipulation to Analyze Pheromone Responses: Knockouts of Multiple Receptor Genes
Measuring Consequences of Protein Misfolding and Cellular Stress Using OMICS Techniques
Multiple Locus Variable Number of Tandem Repeats Analysis
供体
Single-Cell Microinjection Technologies
Pheromone Halo Assay
Functional Liposomal Membranes for Triggered Release
Functional Liposomal Membranes for Triggered Release
Immobilization of Candida rugosa Lipase on Superparamagnetic Fe3O4 Nanoparticles for Biocatalysis in
Isolation and Characterization of Human Hair Follicle Epithelial Cells
DNA and RNA Ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3)
Immunofluorescence Analysis of Human Spermatocytes
Evaluating Spindle Assembly Checkpoint Competence in Mouse Oocytes Using Immunoblotting
Measurement of GFP Expression and DNA Content in Permeabilized Cells
Ex Utero Culture and Live Imaging of Mouse Embryos
Affinity Chromatography: History, Perspectives, Limitations and Prospects
Rho GTPases in Hematopoietic Stem/Progenitor Cell Migration
Analysis of Chromatin Structure at Meiotic DSB Sites in Yeasts
Two-Color In Situ Hybridization of Whole-Mount Mouse Embryos
Bone Marrow-Derived Endothelial Progenitor Cells: Isolation and Characterization for Myocardial Repa
关于RPMI1640细胞培养液的配制
Isolation of Human Male Germ-Line Stem Cells Using Enzymatic Digestion and Magnetic-Activated Cell S
细胞运输(cellular transport)
Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells
Analysis of Oligonucleotides Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry
Imaging mRNAs in Living Mammalian Cells
Quantitative Analysis of Somatic Mitochondrial DNA Mutations by Single-Cell Single-Molecule PCR
Role of Natural Killer Cells and Killer Immunoglobulin-Like Receptor Polymorphisms: Association of H
Large Scale Purification of Brain Tubulin With the Modified Weisenberg Procedure
How to perform automated counts of fluorescently stained cells.
Practical Considerations of Membrane Protein Instability during Purification and Crystallisation
Arginine and Macrophage Activation
High-Yield Isolation and Subcellular Proteomic Characterization of Nuclear and Subnuclear Structures
The Identification of Dengue Virus Using PCR
Neural Differentiation of Human Adipose Tissue-Derived Stem Cells
个别组织细胞的培养
Phylogenomic Resources at the UCSC Genome Browser
Cellular Populations Isolated from Newborn Mouse Skin Including Mesenchymal Stem Cells
Automated Pattern Ranking in Differential Display Data Analysis
Human Mononuclear Phagocytes in Tissue Culture
Spatiotemporal Regulation of Ras-GTPases During Chemotaxis
Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells
Pax3 and Vertebrate Development
PNA-FISH Technique for In Situ Assessment of Aneuploidy
Screening and Selection Strategies for Disulfide Isomerase Activity
Relative Reverse Transcription-Polymerase Chain Reaction
Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis
Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media
膜结合蛋白(membrane-binding proteins)
Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling
Isolation and Purification of Murine Male Germ Cells
Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential
ATP-Loaded Liposomes for Targeted Treatment in Models of Myocardial Ischemia
Designing RNAi Screens to Identify JAK/STAT Pathway Components
Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems
Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology
Confrontation Cultures of Embryonic Stem Cells With Multicellular Tumor Spheroids to Study Tumor-Ind
Phylogenomic Analysis by Chromosome Sorting and Painting
Inducible Expression in Human Cells, Purification, and In Vitro Assays for the Mitochondrial DNA Hel
High Cell-Density Fermentation
Locomotor Activity in Rodents
Chemical Induction of Apoptosis
Human Embryonic Stem (ES) Cell Protocols――Thawing Human ES cells
Co-IP免疫共沉淀实操注意事项
Co-IP免疫共沉淀实验常见问题及解决方法
Northern Blot印迹杂交实验介绍
Northern Blot印迹杂交实验详细操作步骤
Southern Blot印迹杂交实验介绍
Southern Blot印迹杂交实验操作步骤
Southern blot印迹杂交实验常见问题与注意事项
EMSA凝胶迁移实验原理介绍
EMSA实验详细操作步骤
EMSA凝胶迁移实验常见问题与注意事项
石蜡切片实验介绍
石蜡切片制作步骤
石蜡切片制作过程中的注意事项
石蜡切片制作常见问题与解决方法【上】
石蜡切片制作常见问题与解决方法【下】
Microarray Data Analysis in Studies of Membrane Transporters
Gene Expression Profiling of Mouse Epidermal Keratinocytes
Stable Integration of Vectors at High Copy Number for High-Level Expression in Animal Cells
Solid Phase Assays for Studying ECM Protein-Protein Interactions
Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy
Cryoelectron Microscopy of Vitreous Sections: A Step Further Towards the Native State
Exploiting the Liberation of Zn2+ to Measure Cell Viability
将构建的真核表达载体转染进HEK293细胞的方法
Molecular Cytogenetic Applications in Diagnostics and Research: An Overview
Differentiation of Dendritic Cells from Human Embryonic Stem Cells
DNA甲基化实验介绍
The Application of Differential Display to the Brain: Adaptations for the Study of Heterogeneous Tis
DNA甲基化实验操作步骤
Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Built-In Pillar Array
Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes
六种甲基化检测方法
Fluorescent Differential Display
研究新热点之细胞焦亡
Analysis of Janus Tyrosine Kinase Phosphorylation and Activation
细胞焦亡的研究方法与检测方式
EMS Screens: From Mutagenesis to Screening and Mapping
Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells
Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos
细胞焦亡在临床各领域的研究现状
In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET
Quantitative Fluorescence In Situ Hybridization on Paraffin Embedded Tissue
Fluorescence Immunohistochemistry in Combination with Differential Interference Contrast Microscopy
Exploring the Link Between Human Embryonic Stem Cell Organization and Fate Using Tension-Calibrated
白色体(etioplast)
原位杂交实验
Difficult Proteins
Immunocytochemistry on Suprachiasmatic Nucleus Slices
组织FISH实验操作步骤
Methods for Data Analysis
荧光原位杂交实验操作步骤
Micellar Electrokinetic Chromatography of Aminoglycosides
Computational Modeling of Signal Transduction Networks: A Pedagogical Exposition
常用细胞凋亡检测方法（五）
Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology
Identification and Quantitation of Cocaine, Benzoylecgonine, and Cocaethylene in Blood, Serum, and P
Rap1 and Integrin Inside-Out Signaling
Fluorometric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis
High-Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Media
Aging of Cultured Human Skin Fibroblasts
原位杂交实验操作注意事项
Gametic Cell Adhesion and Fusion in the Unicellular Alga Chlamydomonas
原位杂交实验操作常见问题答疑
Fusion and Cloning
裸鼠皮下成瘤实验步骤与介绍
Prokaryotic Phototaxis
裸鼠皮下成瘤实验操作注意事项
Analysis of mRNA Expression During Preimplantation Development
裸鼠皮下成瘤实验常见问题处理方法
Histological Processing of Teeth and Periodontal Tissues for Light Microscopy Analysis
冰冻切片实验介绍
Infection with retroviruses
冰冻切片详细操作步骤
General Linear Models
Chromatin Immunoprecipitation Technique for Study of Transcriptional Dysregulation in Intact Mouse B
冰冻切片操作注意事项
High-Throughput Screen for Aromatic Hydroxylation
Evaluation of Circulating Endothelial Precursor Cells in Cancer Patients
冰冻切片制片的影响因素
冰冻切片与石蜡切片的区别
酵母遗传学方法11：PCR介导基因破坏法
什么是HE染色法？
联会(synapsis)
Promoter Analysis in Zebrafish Embryos
HE染色实操注意事项
In Situ Hybridization to Plant Tissues and Chromosomes
Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro
HE染色常见问题与处理方法
HE染色实验问题答疑
免疫组化实验技术介绍【
免疫组化/IHC实验操作步骤
免疫组化/IHC实验操作注意事项
免疫组化（IHC）染色常见问题及其解决方法上
免疫组化/IHC染色常见问题及其解决方法下
免疫组化和免疫荧光有什么区别？
什么是免疫荧光技术？
细胞/组织免疫荧光实验操作步骤
免疫荧光实操注意事项汇总
免疫荧光常见问题及其解决方法
什么是细胞增殖？
细胞增殖检测方法汇总
Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Nativ
Isolation and Characterization of Cancer Stem Cells In Vitro
Cell-Based Co-transfection Microarrays for Use with HEK293T Cells on a Poly d-Lysine-Coated Polystyr
Mosaic Analysis in Caenorhabditis elegans
Fine Structure of the Autophagosome
Antisense Therapeutics in the Central Nervous System: The Induction of c-fos
Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Subtrates
Determination of Gene Promoter Activity in Skeletal Muscles In Vivo
Single Molecule Tracking for Studying Nucleocytoplasmic Transport and Intranuclear Dynamics
Application of Differential Display in Studying Developmental Processes
Preparation of RNA from Cultured Cells
Fertilization and Sperm Chemotaxis in Ascidians
Derivation of High-Purity Oligodendroglial Progenitors
A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA
Growth and Differentiation of Human Adipose Stromal Cells in Culture
旁分泌细胞（paracrine cell）
Primary Cell Cultures for the Study of Myelination
Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents
Bioluminescence: Imaging Modality for In Vitro and In Vivo Gene Expression
High-Yield Purification of Functionally Active Glucocorticoid Receptor
Genetically-Encoded Fluorescent Probes for Imaging Endogenous mRNA in Living Cells
Gene Therapy Using Neural Stem Cells
Inducible Gene Expression in Transient Transgenic Xenopus Embryos
肌质网(sarcoplasmic reticulum)
猪白细胞介素-2（IL-2）酶联免疫分析（ELISA）
Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes
Studying G2 DNA Damage Checkpoints Using the Fission Yeast Schizosaccharomyces pombe
In Vitro Assays for Tumors Grown In Vivo: A Review of Kinetic Techniques
Intracellular ATP Delivery Using Highly Fusogenic Liposomes
Fluorescence Imaging of Mitochondria in Yeast
Characterizing the Phenotype of Murine Epidermal Progenitor Cells: Complementary Whole-Mount Visuali
Conditional Gene Knockout Using Cre Recombinase
Assays of Lipogenic Enzymes
Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors
Video-EEG Monitoring Methods for Characterizing Rodent Models of Tuberous Sclerosis and Epilepsy
细胞复苏后贴壁细胞较少的问题分析
LPS介导的MAPK信号通路
专为转染HEK 293细胞而设计的转染试剂盒
MAPK级联信号通路图
IP3受体(IP3 receptor)
High Resolution PCR Quantitation by AmpliSensor Assay
Analysis of Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis
In Situ Zymography
An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1
Reversion of Mouse Postimplantation Epiblast Stem Cells to a Nave Pluripotent State by Modulation of
Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases
Detection and Characterization of Leishmania Parasites by DNA-Based Methods: Southern Blotting and P
Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay
氧还电位(oxidation-reduction potentials, redox potentials)
Assessing Bad Sub-cellular Localization Under Conditions Associated with Prevention or Promotion of
Oocytes Microinjection Assay to Study the MAP-Kinase Cascade
细胞复苏之我见－经验总结
几种细胞培养基M199中维生素含量的测定方法
植物多倍体诱发实验
“黑胶虫”问题
Onco-CYTE(TM):不需要FBS的细胞培养基
核小体(nucleosome)
大鼠原代肾小管上皮细胞培养方法
关于细胞培养中支原体污染的经验分享
Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein
细胞核与线粒体的分级分离
Matrigel invasion assays
Quantitation of Iothalamate in Urine and Plasma Using Liquid Chromatography Electrospray Tandem Mass
Metaphase chromosome preparation
Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Act
间隔基因(gap genes)
造血干细胞(hematopoietic stem cell，HSC)
受体钝化(receptor desensitization)
Tunel Procedure in Bovine Embryos 牛胚胎TUNEL检测凋亡
光系统Ⅰ复合物(photosystem Ⅰcomplex, PSⅠcomplex)
ELISA实验中血清标本的采集、保存及注意事项
Transcriptional Regulatory Mechanisms That Govern Embryonic Stem Cell Fate
Polyethylene Glycol-Mediated Cell Fusion
染色体组型分析
Comparative Purification Strategies for Drosophila and Human Mitochondrial DNA Replication Proteins:
红霉素(Erythromycin)酶联免疫分析（ELISA）
Selection of Transfected Suspension Cells
外核膜(outer nuclear membrane)
细胞培养基中的添加剂及其作用
人类人工染色体的构建策略
Apoptosis TUNEL Assay (frozen sections)
奢侈基因(luxury gene)
Membrane Protein Solubilization
Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells
A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Respo
Matrigel invasion assays
Microscale Thermophoresis for the Assessment of Nuclear Protein-Binding Affinities
Cryopreservation of Human Red Blood Cells
A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays
Immobilization of Enzymes on Glyoxyl Agarose: Strategies for Enzyme Stabilization by Multipoint Atta
Bovine Somatic Cell Nuclear Transfer
Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells
Migration Assays for Oligodendrocyte Precursor Cells
self-assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses
Localization of rDNA at Nucleolar Structural Components by Immunoelectron Microscopy
Isolation and Analyses of Enriched Populations of Male Mouse Germ Cells by Sedimentation Velocity: T
Plant Mitochondrial Transcriptomics by Quantitative RT-PCR
Isolation of Mesenchymal Stem Cells from Murine Bone Marrow by Immunodepletion
Solid Tumor Cancer Markers and Applications to Steroid Hormone Research
Specific PCR Primers for the Identification of Endomycorrhizal Fungi
Combined Total Proteomic and Phosphoproteomic Analysis of Human Pluripotent Stem Cells
Forkhead (FOX) Transcription Factors and the Cell Cycle: Measurement of DNA Binding by FoxO and FoxM
Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Rec
Full Sequencing of Viral Genomes: Practical Strategies Used for the Amplification and Characterizati
Culture of Megakaryocytes and Platelets from Subcutaneous Adipose Tissue and a Preadipocyte Cell Lin
CELL CYCLE ANALYSIS
核内不均一RNA
接触抑制(contact inhibition)
Identification of Musashi1-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by
Exploring RNA Virus Replication in Xenopus Oocytes
Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery
In Situ Hybridization to Polytene Chromosomes of Drosophila melanogasterand Other Dipteran Species
Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins
Assessing In Vivo MicroRNA Function in the Germline Stem Cells of the Drosophila Ovary
Analysis of Gene Expression in Skin Using Laser Capture Microdissection
Fluorescence In Situ Hybridization for Molecular Cytogenetic Analysis in Filamentous Fungi
Immunofluorescence Staining of Spindles, Chromosomes, and Kinetochores in Human Oocytes
Free-Hand Bisection of Mouse Oocytes and Embryos
SOLID TUMOR CULTURES FOR CHROMOSOME ANALYSIS
Measurement of Apoptotic Cell Clearance In Vitro
Adenoviral Transduction of Mesenchymal Stem Cells
Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells
Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies: Real-Time Detection
Essentials of Recombinase-Based Genetic Fate Mapping in Mice
Assessing Chronological Aging in Bacteria
Cell-Adhesion Assays: Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Mem
Analysis of Lipoplex Structure and Lipid Phase Changes
In Situ Hybridization Using Riboprobes on Free-Floating Brain Sections
Tyramide Signal Amplification for DNA and mRNA In Situ Hybridization
Induction of Deoxyribonucleic Acid Damage by Irradiation
Messenger RNA Turnover in Cell-Free Extracts from Higher Eukaryotes
Penetratin Story: An Overview
Gene Silencing Using RNA Interference in Embryonic Stem Cells
Bisulfite Treatment of DNA
无血清培养基及其使用方法概述
细胞培养设施和基本条件
Nuclear Receptors: One Big Family
Tissue Engineering and Cell-Populated Collagen Matrices
Monitoring RNALigand Interactions Using Isothermal Titration Calorimetry
Use of Peptide Libraries to Determine Optimal Substrates of Tyrosine Kinases
Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondri
Purification of Human PARP-1 and PARP-1 Domains from Escherichia coli for Structural and Biochemical
Thermodynamics of Lipid Interactions with Cell-Penetrating Peptides
Whole Body MRI and Fluorescent Microscopy for Detection of Stem Cells Labeled with Superparamagnetic
In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method
Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Mu
Treatment of Liver Tumors in Rabbit
Analysis of Chromosome Dynamics and Chromosomal Proteins in Drosophila Spermatocytes
Imaging of Schwann Cells In Vivo
Transplantation of Chimeric Fetal Liver to Study Hematopoiesis
Immobilization of Enzymes Acting on Macromolecular Substrates: Reduction of Steric Problems
Lentiviral Vector-Mediated Gene Transfer and RNA Silencing Technology in Neuronal Dysfunctions
Measuring Ca2+ Oscillations in Mammalian Eggs
Transition Metal Methods for Immobilization of Enzymes and Cells
Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Tech
Cell Fusion in the Filamentous Fungus, Neurospora crassa
Collagen Gel Assay for Angiogenesis: Induction of Endothelial Cell Sprouting
Mesenchymal Stem Cells and Nano-structured Surfaces
Noninvasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs
Tissue Culture Correlational Study of Genetic Cholangiopathy of Autosomal Recessive Polycystic Kidne
From Gene-Scale to Genome-Scale Phylogenetics: the Data Flood In, but the Challenges Remain
Study Design: The Basics
Mouse Models of Triplet Repeat Diseases
Detection of Cytoplasmic and Nuclear Functions of mTOR by Fractionation
Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis
Live Imaging for Studying Asymmetric Cell Division in the C. elegans Embryo
Organotypic raft culture of primary human keratinocytes (PHKs)
Application of Plastic Embedding for Sectioning Whole-Mount Immunostained Early Vertebrate Embryos
Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization
Analysis of Cell Death by Electron Microscopy
Imaging of Embryonic Stem Cell Migration In Vivo
非内共生学说
流式细胞术:标本处理
PI3K-Akt信号通路的抑制位点
Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader
Hematopoietic and Nature Killer Cell Development from Human Pluripotent Stem Cells
Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes
粘着带(adhesion belt)
Entrapment in Calcium Alginate
大鼠成纤维细胞生长因子（FGF）酶联免疫分析
Genetic Complementation Protocols
An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics
Radioligand-Binding Methods for Membrane Preparations and Intact Cells
Quantifying Amplicons with ELISA
Isolation of Microtubules (Bovine Brain)
Screening for Thermostability
外周血培养基的配制
cck8法检测细胞增殖详细操作步骤
细胞增殖检测之MTT法
EDU法检测细胞增殖操作
细胞增殖检测的注意事项
什么是彗星实验？
彗星实验/单细胞凝胶电泳实验操作步骤
彗星实验/单细胞凝胶电泳实验注意事项
彗星实验操作常见问题答疑
细胞衰老的介绍与研究意义是什么？
细胞衰老的检测方法
贴壁细胞、悬浮细胞、组织切片衰老实验操作步骤
什么是实时荧光定量PCR？
荧光定量PCR实验操作步骤
荧光定量PCR结果分析解读
荧光定量PCR实操注意事项
实时荧光定量PCR常见问题及解决方法
实时荧光定量PCR实验技术答疑
什么是SNP/单核苷酸多态性？
SNP/单核苷酸多态性检测最常用的三种方法
SNP检测方法总结
Taqman探针法检测SNP分型详解
直接测序法检测SNP详解——最常用的方法
SNP检测常见问题答疑上篇
SNP检测常见问题答疑下篇
什么是血管生成实验？
血管生成实验操作步骤
什么是细胞培养？
动物细胞原代培养操作步骤
动物细胞传代培养详细步骤
植物组织的培养方法
植物细胞的培养方法总结
细胞培养/细胞实验操作注意事项
细胞培养实常见问题及处理汇总
细胞冻存与复苏的操作步骤
细胞冷冻与复苏操作注意事项
细胞冻存与复苏问题汇总大全
细胞培养基的理化性质和基本成分
细胞操作的无菌环境与操作要点
什么是细胞划痕实验？
细胞划痕实验操作详细步骤
细胞划痕实验操作注意事项
细胞划痕实验操作问题答疑
Tunel染色实验原理与步骤详解
Tunel染色实操注意事项
Tunel染色实验的常见问题与解决方法
什么是酵母双杂系统？
酵母双杂详细操作步骤
酵母双杂实验注意事项
酵母双杂交常见问题及解决方法上
酵母双杂交常见问题及解决方法下
Optical Imaging of Cell Fusion and Fusion Proteins in C aenorhabditis elegans
Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short H
Rat Osteoblast Cultures
Electron Microscopy and In Situ Hybridization: Expression of P2Y2 Receptor mRNA in the Cerebellum
Embryological Methods in Ascidians: The Villefranche-sur-Mer Protocols
半桥粒(hemidesmosomes)
Endogenous Biotin in Rat Brain: Implications for False-Positive Results With AvidinBiotin and Strept
Studying the Structure of Microtubules by Electron Microscopy
Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNA-Lipid Complexes
RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 cells
Monitoring Integrin Activation by Fluorescence Resonance Energy Transfer
光呼吸(photorespiration)
CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors
人脱氧吡啶啉（DPD）酶联免疫分析（ELISA）
In Vitro Derivation and Expansion of Endothelial Cells From Embryonic Stem Cells
Mammalian Cell Microinjection Assay to Study the Function of Rho Family Guanosine Triphosphatases
Assaying Cytochrome c Translocation During Apoptosis
Differential Screening of Differential Display cDNA Products by Reverse Northern
Imaging B-Cell Receptor Signaling by Single-Molecule Techniques
Revised protocol for establishing a mammary cell line from tumors
Analysis of Chaperone Properties of Small Hsp
Ligand Binding and Processing: The Perfused Liver as a Model System
Improving Lipase Activity by Immobilization and Post-immobilization Strategies
Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard
核周腔(perinuclear space)
Double Indirect-Immunofluorescent Labeling of Cultured Cells
Analysis of Lipid Profiles in Skeletal Muscles
Quantification of Genomic Mutations in Murine Hematopoietic Cells
Indirect Immunofluorescence for Monitoring Spindle Assembly and Disassembly in Yeast
Identification of Cryptic Sex Chromosomes and Isolation of X- and Y-Borne Genes
Studies of Proteoglycan Involvement in CPP-Mediated Delivery
Capillary Electrophoretic Analysis of Organic Pollutants
The Use of Embryonic Stem Cells to Study Hedgehog Signaling
Cell Migration: An Overview
Germline Clone Analysis for Maternally Acting Drosophila Hedgehog Components
Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System T
Clustering Assay for Studying the Interaction of Membrane Proteins with PDZ Domain Proteins
Microtiter-Plate Assay and Related Assays for Nonspecific Endonucleases
Deriving Mouse Spermatogonial Stem Cell Lines
Immobilization of Cells Using Electrostatic Droplet Generation
In Vitro Electrophysiological Mapping of Stem Cells
Use of In Vivo Gap Repair for Isolation of Mutant Alleles of a Checkpoint Gene
BacMam-Mediated Gene Delivery into Multipotent Mesenchymal Stromal Cells
Laser Desorption Ionization Mass Spectrometry of Bioorganic Molecules
Mammalian Expression Systems and Transfection Techniques
Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry
Evaluation of Skull and Tooth Morphology and Mineralization Using High-Resolution X-Ray Tomography
Isolation of Common Dendritic Cell Progenitors (CDP) from Mouse Bone Marrow
Hollow Fiber Assay for Tumor Angiogenesis
大规模培养常用方法
Formation of Gut-Like Structures In Vitro From Mouse Embryonic Stem Cells
动物染色体标本的制备
Affinity Chromatography of Phosphorylated Proteins
Immobilization of Enzymes by Selective Adsorption on Biotinylaminopropyl Celite or Glass
Keratinocyte Culture in the Absence of Substrate Attachment
Isolation and Propagation of Colon Cancer Stem Cells
Extracorporeal Assays of Thrombosis
Live Cell Fluorescence Microscopy Techniques
FRAP Analysis of Chemosensory Components of Dictyostelium
Multiplex Gene Expression Analysis by TRAC in Fungal Cultures
Angiogenic Vessel-Targeting DDS by Liposomalized Oligopeptides
Palate Development: In Vitro Procedures
ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy
Evaluation of VEGF-Induced Vascular Permeability in Mice
Comparative Pharmacokinetics of Antisense Oligonucleotides
Health and Neonatal Care of Bovine Clones
Oxidative Damage in Huntington’s Disease
cRNA Probes: Comparison of Isotopic and Nonisotopic Detection Methods
New Protocol for Lentiviral Vector Mass Production
Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation
The Genome of Dictyostelium discoideum
Methods for Assessing Effects of Wnt/-Catenin Signaling in Senescence of Mesenchymal Stem Cells
Imaging Cells Within 3D Cell-Derived Matrix
Purification of Proteins Using Displacement Chromatography
Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Site-Directed Mutagenesis to Study the StructureFunction Relationships of Ion Channels
Preparation of Immobilized Subunits of a Multisubunit Enzyme
MTT Cell Proliferation Assay
Gene Expression Analyzed by Ribonuclease Protection Assay
Methods for Gene Expression Profiling in Dermatology Research Using DermArray Nylon Filter DNA Micro
Serial Section Immunoelectron Microscopy of Algal Cells
Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants
中心粒(centrioles)
小鼠白细胞介素-6（IL-6）酶联免疫分析（ELISA）
Transfection of Mammalian Cells Using Lipofectamine
Redox Property of Ribonucleotide ReductaseSmall Subunit M2 and p53R2
Confocal Microscopy of Adipocytes
Using Intrinsic Fluorescence Emission Spectroscopy to Study Steroid Receptor and Coactivator Protein
细胞培养技术注意事项大汇总
MTT的升级替代产品——CCK-8
Histochemical Localization of Apoptosis With In Situ Labeling of Fragmented DNA
An Affinity Oligonucleotide Displacement Strategy to Purify Ribonucleoprotein Complexes Applied to H
Purification and Mass-Spectrometry Identification of Microtubule-Binding Proteins from Xenopus Egg E
细胞融合实验流程
细胞融合实验流程
Protein Import Into Isolated Mitochondria
In Vivo Optical Imaging of Ischemic BloodBrain Barrier Disruption
Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry
Assessment of Impaired Proteasomal Function in a Cellular Model of Polyglutamine Diseases
微染色体－微管结合实验（Minichromosome
Cell counting/plating
遗传学实验之——染色体组型分析
简单扩散(simple diffusion)
P-Element Mutagenesis
Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phos
Methods for the Assessment of Telomere Status
Utilization of Digital Differential Display to Identify Novel Targets of Oct3/4
Nucleotide Excision Repair Assay in Drosophila melanogaster Using Established Cell Lines
Culturing Human Schwann Cells
Chemokine Receptor Dimerization and Chemotaxis
Application of FISH to Archival Cytogenetic Slides
Interaction of Genetic and Environmental Factors in Saccharomyces cerevisiae Meiosis: The Devil is i
什么是酵母单杂实验？
酵母单杂交实验详细步骤
酵母杂交实验的常见问题—转化篇
酵母杂交实验的常见问题—试剂篇
什么是RNA pulldown实验？
RNA pull-down详细实验步骤
RNA pull-down注意事项与常见问题
RNA pull-down实验问题答疑
DNA pull-dwon实验介绍
DNA pulldown实验操作步骤
DNA pulldown实验问题答疑
GST pulldown实验原理与介绍
GST pulldown实验操作步骤
什么是耐药细胞株？
耐药细胞株构建详细步骤步骤
耐药细胞株构建注意事项
脓毒症动物模型介绍
脓毒症模型构建操作步骤
非酒精性脂肪肝造模原理与方法
非酒精性脂肪肝造模详细步骤
小鼠肠炎造模实验介绍
小鼠肠炎造模操作步骤
什么是糖尿病动物模型？
A Novel Role for Nutrition in the Alteration of Functional Microdomains on the Cell Surface
糖尿病动物模型造模步骤
Insect Pheromone Behavior: Fruit Fly
糖尿病动物造模注意事项
Methods of Telomerase Inhibition
肝纤维化动物造模机制介绍
Screening of Nanoparticle Embryotoxicity Using Embryonic Stem Cells
Reproductive and Developmental Manipulation of the Marsupial, the Tammar Wallaby Macropus eugenii
肝纤维化动物造模步骤上篇
Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry
Analytical Methods for Cancer Stem Cells
Assessing BloodBrain Barrier Function Using In Vitro Assays
Separation of Synthetic (Co)Polymers by Capillary Electrophoresis Techniques
Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR)
合成代谢（anabolism）
Cell Disruption and Subcellular Fractionation
Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye
Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis
Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions
肝纤维化动物造模步骤下篇
羊水细胞染色体标本的制备
Mimicry of Protein Function with Cell-Penetrating Peptides
关节炎动物造模原理与介绍
人褪黑素 (melatonin)酶联免疫分析
关节炎动物造模方法介绍
In Vitro Production of Enucleated Red Blood Cells from Hematopoietic Stem and Progenitor Cells
Cell-Cell Adhesive Interactions in an In Vitro Flow Chamber
关节炎动物模型造模步骤
Efficient Isothermal Amplification of the Entire Genome from Single Cells
脑缺血模型实验介绍
Analysis of Apoptosis by Flow Cytometry
脑缺血模型造模步骤—上
Method: Logging in Specimens and Record Keeping
Monitoring Aggregate Formation in Organotypic Slice Cultures From Transgenic Mice
一些干细胞标志物
Recording Dendritic Ion Channel Properties and Function from Cortical Neurons
Biological Membranes as Protein Aggregation Matrices and Targets of Amyloid Toxicity
Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Developm
Live-Cell Imaging of Microtubule Dynamics in Hyphae of Neurospora crassa
egakaryocyte and Platelet Production from Human Cord Blood Stem Cells
Assessment of Oxidative Stress in Sperm and Semen
Molecular Profiling of Mitochondrial Dysfunction in Caenorhabditis elegans
脑缺血模型造模步骤—下
脑缺血动物造模实操注意事项
活性氧（ROS）检测介绍
活性氧检测荧光法实验步骤
活性氧检测化学发光法实验步骤
什么是支原体检测？
支原体检测方法大全
PCR检测支原体操作步骤
培养法检测支原体操作步骤
DNA染色法检测支原体操作步骤
Dot blot斑点杂交实验原理与方法
RNA Dot Blot斑点杂交实操步骤
淋巴细胞亚群检测实验原理与介绍
Th淋巴细胞亚群检测步骤
什么是粒细胞？
粒细胞检测实验原理与流程
什么巨噬细胞？
Isolation, Characterization, and Culture of Normal Human Intestinal Crypt and Villus Cells
Phosphoinositides, Inositol Phosphates, and Phospholipase C in Embryonic Stem Cells
Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cas
Molecular Cytogenetics: The Standard FISH and PRINS Procedure
Molecular Links Between Autophagy and Apoptosis
Live Imaging of Primitive Endoderm Precursors in the Mouse Blastocyst
Identification of Novel Genes Involved in Adipose Differentiation by Differential Display
Quantitation of Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine Using Gas Chromatog
Association Methods in Human Genetics
Analysis of LINE-1 Expression in Human Pluripotent Cells
小鼠白细胞介素-10（IL-10）酶联免疫分析（ELISA）
Characterization of Constitutively Active Mutants of G Protein-Coupled Receptors
The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components
重复序列(repetitive sequence)
Cell-Specific Aptamers for Targeted Therapies
Adenovirus General Protocol for Infecting Cells
牙周膜细胞和牙髓细胞的培养方法
Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods
Salt-Urea, Sulfopropyl-Sepharose, and Covalent Chromatography Methods for Histone Isolation and Frac
Cell Surface Engineering of Mesenchymal Stem Cells
伪装的mRNA（masked mRNA）
Measuring Programmed Cell Death in Plants
细胞因子信号网络图(5张)
FISH for Mapping Single Copy Genes
Ultrastructural and Immunofluorescent Methods for the Study of the XY Body as a Biomarker
Embryonic Stem Cells as a Model for Studying Melanocyte Development
微丝(microfilament)
Fusion Assays and Models for the Trophoblast
Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Ta
Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal
Separation of Peptides by Capillary Electrophoresis
骨髓细胞染色体标本制备
Yeast Mitochondrial Transcriptomics
Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins
Lentivirus-Based Virus-Like Particles as a New Protein Delivery Tool
Cell Membrane Vesicles as a Tool for the Study of Direct EpithelialStromal Interaction: Lessons from
Fluorescence-Based Tl+Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca
A Method to Isolate and Culture Expand Human Dental Pulp Stem Cells
Cell Culture of Primary Cerebellar Granule Cells
Isolation of Primary Avian Osteocytes
Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cells
Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs
Expression of Tetanus Toxin Fragment C
In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors
Electrical Recordings from the Accessory Olfactory Bulb in VNO-AOB Ex Vivo Preparations
Making cRNA for Microinjection and Expression of Fluorescently Tagged Proteins for Live-Cell Imaging
Ex Vivo Stromal Cell Electroporation of Factor IX cDNA for Treatment of Hemophilia B
Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Bra
Methods for the Study of Entosis
Real-Time Spectrofluorometric Assays for the Lumenal Environment of the Maturing Phagosome
Culture Systems: Sequential
Identification of Target Membrane Proteins as Detected by Phage Antibodies
Nitrocellulose Filter Binding for Determination of Dissociation Constants
Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/A
Detection of Chromatin-Bound PCNA in Cultured Cells Following Exposure to DNA-Damaging Agents
Ex Vivo Organ Culture of Human Hair Follicles: A Model EpithelialNeuroectodermalMesenchymal Interact
Sequencing of Viral Genes
Methods to Determine the Status of Mitochondrial ATP Synthase Assembly
Measurement of Adipose Tissue Blood Flow
大鼠胆固醇（CH）酶联免疫分析
SpermEgg Fusion Assay in Mammals
Preparation, Characterization, and Use of Antioxidant-Liposomes
Genetic Modification of Chondrocytes Using Viral Vectors
三联管(triplet)
Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut
Principles for Derivation of Human Embryonic Stem Cells
Protocols for Studying Antizyme Expression and Function
The efficient generation of mesenchymal cells such as adipocytes, osteoblasts, and chondrocytes from
Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells
Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium
In Vitro Human T Cell Development Directed by NotchLigand Interactions
干细胞(stem cells, SC)
Detecting Expression Patterns of Wnt Pathway Components in Nematostella vectensis Embryos
巨噬细胞培养方法与步骤
巨噬细胞分离与纯化步骤
巨噬细胞极化实验步骤
巨噬细胞流式检测方法
什么是亚硫酸氢钠测序？
亚硫酸氢钠测序（BSP）实验步骤
单细胞悬液制备方法—下
什么是组织芯片？
组织芯片详细制作步骤
组织芯片制作注意事项
甲醇刚果红染色实验介绍
刚果红染色实验步骤
动物组织样本DNA提取步骤
植物组织样本DNA提取步骤
考马斯亮蓝染色法实验介绍
TTC染色实验介绍
TTC染色实验步骤
什么是番红固绿染色？
动物组织番红固绿染色步骤
植物组织番红固绿染色步骤
什么是RIP检测？
RIP检测实验操作步骤
RIP检测样本制备注意事项
动物组织甲苯胺蓝染色步骤
什么是外泌体？
外泌体提取步骤
外泌体鉴定的三个标志与应用
组织切片脱水后不能及时包埋怎么办？
细胞划痕实验最佳设置时间是多久？
taqman探针两段带有什么基因？
实验用的植物组织蛋白样本怎样提取出来？
肿瘤组织怎样提取蛋白？
组织做冰冻切片一般是什么温度比较合适？
做石蜡切片时石蜡带弯曲不直怎么办？
什么是免疫荧光的半定量分析？
石蜡切片碎掉的蜡块可以重新包埋吗？
做石蜡切片时组织总是碎裂怎么办？如何避免？
石蜡切片总是厚薄不均匀怎么办？
石蜡切片染色不均匀模糊怎么办？
什么是单细胞悬液？有什么用？
小鼠成瘤不理想可以多次接种吗？
什么是荧光定量pcr三步法？
QPCR中的CT值是什么意思？
QPCR荧光定量基因表达量太低跑不出来怎么办？
冰冻切片染色模糊不清什么原因？
双荧光素酶实验细胞一般转染多久？
免疫荧光染不上色怎么办？
彗星实验结果怎么分析？
双荧光素酶的两个报告基因载体作共转染应用什么条件呢？
裸鼠成瘤肿瘤为什么长起来又消失了？
High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR
CDK-Activating Kinases: Detection and Activity Measurements
Visualization of mRNA Localization in Xenopus Oocytes
常规组织初代消化培养
The Human Embryo: Ethical and Legal Aspects
MT Spindowns from Extracts
Identification and Quantitation of G-Protein α-Subunits
In Vivo Measurement of Glioma-Induced Vascular Permeability
细胞培养常见问题、可能原因及解决方法
Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extre
Traditional Human Embryonic Stem Cell Culture
Isolation of the Constitutive Heterochromatin from Mouse Liver Nuclei
Single-Channel Analysis of TRPC Channels in the Podocytes of Freshly Isolated Glomeruli
基因疫苗 Gene Vaccine
次级溶酶体(secondary lysosome)
Neural Stem Sphere Method: Induction of Neural Stem Cells and Neurons by Astrocyte-Derived Factors i
Visualisation of RNA by Electron Microscopic In Situ Hybridisation
顶体(acrosome)
Periodic Activation of ERK2 and Partial Involvement of G Protein in ERK2 Activation by cAMP in Dicty
Neoepitope Antibodies Against MMP-Cleaved and Aggrecanase-Cleaved Aggrecan
Simultaneous DNA/RNA Analysis of Solid and Hematoreticular Malignancies
Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports
人角化细胞生长因子(KGF)酶联免疫分析（ELISA）
Archaebacterial Tetraetherlipid Liposomes
Extraction and Immunoblotting of Proteins From Embryos
Isolation of Muscle Stem Cells by Fluorescence Activated Cell Sorting Cytometry
Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronect
Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayers
Analysis of the Cell Cycle in Mouse Embryonic Stem Cells
Basic Bayesian Methods
The Macrophage
In Vitro Construction of 2D and 3D Simulations of the Murine Hematopoietic Niche
Basic Cell Culture
Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cell
Solubilization of Proteins in 2DE: An Outline
Recording of Ion Channel Activity in Planar Lipid Bilayer Experiments
The Use of Degenerate Primers in Conjunction with Strain and Species Oligonucleotides to Classify On
Cripto Signaling in Differentiating Embryonic Stem Cells
Site-Directed Mutagenesis Using Overlap Extension PCR
Identification and Isolation of Rodent Respiratory Tract Dendritic Cells
Determination of DNA Mutation Load in Human Tissues Using Denaturing HPLC-Based Heteroduplex Analysi
The Xenopus Embryo as a Model System for Studies of Cell Migration
Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs)
In Vivo Measurement of Muscle Protein Synthesis Rate Using the Flooding Dose Technique
Paraformaldehyde Fixation of Cells
Cuticle Preparation of Drosophila Embryos and Larvae
NF-kappaB信号通路（10张）
Clinical Uses of Liver Stem Cells
Proteomic Tools for the Analysis of Cytoskeleton Proteins
A Tutorial on Mathematical Modeling of Biological Signaling Pathways
带蛋白(band protein)
Two-Dimensional Separation of Membrane Proteins by 16-BAC-SDS-PAGE
Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies
Identification of Disease Markers by Differential Display: Prion Disease
LC-MS/MS Analysis of 13 Benzodiazepines and Metabolites in Urine, Serum, Plasma, and Meconium
肌球蛋白(myosin)
Live-Cell Imaging of the Cytoskeleton and MitochondrialCytoskeletal Interactions in Budding Yeast
Analysis of Microtubule Dynamics by Polarized Light
Proteomic Analysis of Caenorhabditis elegans
Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells
Myoblast Fusion in Drosophila
Analysis of Mitochondrial Protein Synthesis in Yeast
Treatment of Murine Transplanted Subcutaneous Tumors Using Systemic Drug Administration
Imaging Lipid Membrane Domains with Lipid-Specific Probes
Clonal Analysis of Hedgehog Signaling in Drosophila Somatic Tissues
Characterizing Ligand-Microtubule Binding by Competition Methods
Spoligotyping for Molecular Epidemiology of the Mycobacterium tuberculosis Complex
Analysis of Ascarosides from Caenorhabditis elegans Using Mass Spectrometry and NMR Spectroscopy
In Vitro JAK Kinase Activity and Inhibition Assays
组织工程（histological engineering）
Isolation of Adult Hippocampal Neural Progenitors
In Vivo Cryotechniques for Preparation of Animal Tissues for Immunoelectron Microscopy
SOFT AGAR ASSAY FOR COLONY FORMATION
Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in He
Assays to Measure p53-Dependent and -Independent Apoptosis
Serial Cultivation of Primary Adult Murine Keratinocytes
Mammalian Cell Microinjection Assay to Study the Function of Rac and Rho
Base Excision Repair Assay Using Xenopus laevis Oocyte Extracts
Instrument-Independent Software Tools for the Analysis of MSMS and LCMS Lipidomics Data
Genome-Wide RNAi Screening to Identify Regulators of Oncogene-Induced Cellular Senescence
Functional Purification of Human and Mouse Mammary Stem Cells
Stem Cell and Neuron Co-cultures for the Study of Nerve Regeneration
Maintenance of Pregnancy in Pigs With Limited Viable Embryos
Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells
Mechanism of Transdermal Drug Delivery by Electroporation
Mathematical Investigation of How Oncogenic Ras Mutants Promote Ras Signaling
Parthenogenesis and Nuclear Transfer in Rabbit Oocytes
Parasexual Genetics Using Axenic Cells
Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein
Methods for Detecting Cells in S Phase
Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry
Protein Expression Profiling
Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcripti
A Genome-wide RNAi Screen for Polypeptides that Alter rpS6 Phosphorylation
b-Galactosidase Color Assay for Cultured Cells
Isolation of Oligodendroglial Cells from Cultured Neural Stem/Progenitors
Derivation and Maintenance of Human Embryonic Stem Cells
Derivation of Mouse Embryonic Stem Cell Lines from Blastocysts Produced by Fertilization and Somatic
Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells
Peptide Structure Determination by NMR
A first step in defining the function of a novel gene is to determine its interactions with other ge
Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
Human Embryonic Stem (ES) Cell Protocols――Splitting Human ES cells on MEFs
Vascular Progenitor Cell Mobilization
Cytochemical Probes of Cycling and Quiescent Cells Applicable to Flow Cytometry
Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization
Quantitative Polymerase Chain Reaction
Chromosome Spreading and Immunofluorescence Methods in Saccharomyes cerevisiae
Fluorescence-Activated Cell Sorting Analysis of Mitochondrial Content, Membrane Potential, and Matri
Glycosylation Profiling of Heterologous Proteins
Mouse Epidermal Keratinocyte Culture
In Vitro Spermatogenesis Using an Organ Culture Technique
Serum Thawing Heat Inactivation
Bacterial Phagosome Acidification Within IFN--Activated Macrophages: Role of Host p47 Immunity-Relat
-Tubulin and Microtubule-Binding Assays
Methods for Cancer Stem Cell Detection and Isolation
动物细胞的基本形态观察和显微测量
In Vitro Selection of Protein and Peptide Libraries Using mRNA Display
Single-Cell PCR Methods for Studying Stem Cells and Progenitors
Mathematics of Experimentally Generated Chemoattractant Gradients
Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture
Visual Assessment of Enzyme Immobilization
Quantitation of Methylmalonic Acid in Plasma Using Liquid Chromatography Tandem Mass Spectrometry
Nuclear Transfer in Sheep
Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extracts
Conjunctiva: Organ and Cell Culture
Combing Genomic DNA for Structural and Functional Studies
神经胶质细胞的制备和培养
Generation and Cloning of Antigen-Specific Human T-Cells
Placenta Trophoblast Fusion
Human Female Reproductive Tract Epithelial Cell Culture
Prospective Isolation of Mesenchymal Stem Cells from Mouse Compact Bone
Culture of Glial Cells from Human Brain Biopsies
Bistability in One Equation or Fewer
Selective Antagonism of Receptor Signaling Using Antisense RNA to Deplete G-Protein Subunits
Palatal Dysmorphogenesis: Quantitative RT-PCR
Primary Culture of Human Renal Proximal Tubule Epithelial Cells and Interstitial Fibroblasts
Study of G Protein-Coupled Receptor/-arrestin Interactions Within Endosomes Using FRAP
Blue Native Polyacrylamide Gel Electrophoresis: A Powerful Diagnostic Tool for the Detection of Asse
Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells
Screening for MAPK Modulators Using an In-Cell Western Assay
Efficient PCR Production of Single-Stranded DNA Sequencing Templates
酵母遗传学方法3：RNA的分离
Differentiation of Circulating Monocytes into Fibroblast-Like Cells
PARAFILM PM996封口膜
二甲基亚砜的用途是什么
如何正确的使用封板膜
Parafilm封口膜的使用
Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development
Maintenance of Cell Culture
大鼠氧化低密度脂蛋白(OxLDL)酶联免疫分析（ELISA）
Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase
A Stochastic PCR Approach for RNA Quantification in Multiple Samples
Quantitative Analysis of Human DNA Sequences by PCR and Solid-Phase Minisequencing
FACS Analysis of the Planarian Stem Cell Compartment as a Tool to Understand Regenerative Mechanisms
Culture Systems: Single Step
Fractionation of the Coxiella burnetii Parasitophorous Vacuole
Designing, Testing, and Validating a Microarray for Stem Cell Characterization
Spermine Synthase Deficiency Resulting in X-Linked Intellectual Disability (SnyderRobinson Syndrome)
基因扩增(gene amplification)
Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2
Quantitative Analysis of JAK Binding Using Isothermal Titration Calorimetry and Surface Plasmon Reso
Isolation, Expansion, and Characterization of Human Islet-Derived Progenitor Cells
Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Open
ECM Degradation Assays for Analyzing Local Cell Invasion
Analyzing Import Intermediates of Mitochondrial Proteins by Blue Native Gel Electrophoresis
Managing Apheresis Complications During the Hematopoietic Stem Cell Collection
Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)
A Historical Perspective of the EGF Receptor and Related Systems
Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-T
核纤层(lamina)
磷脂交换蛋白(phospholipid exchang proteins, PEP)
Quantitative X-ray Imaging of Rodent Bone by Faxitron
Mechanical Properties of Bone Ex Vivo
Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis
Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation
Freezing and Thawing Cultured Cells
The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells
In Vitro Culture of Rodent Embryos During the Early Postimplantation Period
Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets
Crosstalk Coregulation Mechanisms of G Protein- Coupled Receptors and Receptor Tyrosine Kinases
Imaging Ca2+ Signals in Xenopus Oocytes
Identification of MetaboliteRiboswitch Interactions Using Nucleotide Analog Interference Mapping and
Reactive Oxygen Species and Upregulation of NADPH Oxidases in Mechanotransduction of Embryonic Stem
In Vitro Excision Repair Assay in Schizosaccharomyces pombe
Generation of iPS Cells from Human Umbilical Vein Endothelial Cells by Lentiviral Transduction and T
Subculturing Cells
Signal Transduction Inhibitors in Cellular Function
抑瘤粉抗肝部细胞实验研究
Harvesting lymphocytes from Peripheral Blood
Making Protein Immunoprecipitates
Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization
Intravital Imaging of Thrombus Formation in Small and Large Mouse Arteries: Experimentally Induced V
Statistical Inference on Categorical Variables
Microfluidic Techniques for the Analysis of Bacterial Chemotaxis
小鼠粒细胞-巨噬细胞集落刺激因子（GM-CSF）酶联免疫分析
Using Surface Electrodes to Monitor the Electric-Pulse-Induced Permeabilization of Porcine Skin
Derivation and Culture of Mouse Trophoblast Stem Cells In Vitro
Investigating the Origins of Somatic Cell Populations in the Perinatal Mouse Ovaries Using Genetic L
Production of Zebrafish Germline Chimeras from Cultured Cells
Cloning and Mutational Analysis of the Shc-Phosphotyrosine Interaction/Phosphotyrosine-Binding Domai
Use of Chemical Mutagenesis in Mouse Embryonic Stem Cells
Electron Spectroscopic Imaging of the Nuclear Landscape
A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity
Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques
StemBase: A Resource for the Analysis of Stem Cell Gene Expression Data
Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing
Methods for Analyzing MicroRNA Expression and Function During Osteogenic Differentiation of Human Ad
Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization
Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging
亮氨酸拉链基元(leucine zipper motif)
Detection of Apoptosis in Drosophila
Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis
The Use of Real-Time Quantitative PCR for the Analysis of Cytokine mRNA Levels
Solid-Phase Screening Using Digital Image Analysis
Practical Protocols for Lipase Immobilization via SolGel Techniques
Methods for Studying Activation of Matrix Metalloproteinases
大鼠血红蛋白(Hb)酶联免疫分析（ELISA）
Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies
Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Bloo
In Situ Hybridization of Suprachiasmatic Nucleus Slices
15种细胞培养中常用培养基及基本特性
Real-Time BRET Assays to Measure G Protein/Effector Interactions
G418原代细胞的筛选的Protocol
Heme Oxygenase Activity Current Methods and Applications
CHO Centrosome Prep
The GTPase Cycle How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases
In Vitro Human Skin Segmentation and Drug ConcentrationSkin Depth Profiles
Generation of Parabiotic Mice for the Study of DC and DC Precursor Circulation
Human Embryonic Stem (ES) Cell Protocols――Freezing Human ES Cells
Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis
Biolistic Mediated Production of Transgenic Oil Palm
Extraction of Yeast Mitochondrial Membrane Proteins by Solubilization and Detergent/Polymer Aqueous
合子或起始减数分裂（Zygotic or initial meiosis）
Processivity of DNA Repair Enzymes
Since the discovery of RNA interference (RNAi) in Caenorhabditis elegans in 1998, this mechanism has
The RNA Silencing Technology Applied by Lentiviral Vectors in Oncology
Methods of Cellular Senescence Induction Using Oxidative Stress
Generation of Human Osteoclasts from Peripheral Blood
Identification and Quantitation of Amphetamine, Methamphetamine, MDMA, Pseudoephedrine, and Ephedrin
ced-9基因（ced-9 gene）
Production of Immortalized Human Neural Crest Stem Cells
Analysis of Properties of Cilia Using Tetrahymena thermophila
Methods to Fuse Macrophages In Vitro
Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
cenM-FISH Approaches
Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium
Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases
Measuring Interactions Between ECM and TGF-Like Proteins
Dynamic Expansion Culture for Mesenchymal Stem Cells
Cell Viability Assay
染色体分带技术
Density Gradient Separation of Sperm for Artificial Insemination
DNA分子标记在三系杂交水稻育种、品种鉴定、遗传性分析上的应
Methods for Identifying and Studying Genetic Alterations in Hormone-Dependent Cancers
Radioactive In Situ Hybridization to Animal Chromosomes
The Transformed Phenotype
胸腹水染色体的标本制备
线粒体荧光探针信息大全
ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays
专门用于冻存哺乳细胞培养基
脂质体2000可以转染Hela细胞吗
Angiogenic Signalling Pathways
GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro
Isolation of Nucleic Acids
Feeder-Free Substrates for Pluripotent Stem Cell Culture
Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-
胞质分裂(cytokinesis)
Screening Expression Libraries with Solution-Based Assay
Ras GTPases Are Both Regulators and Effectors of Redox Agents
Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies
停靠蛋白(docking protein, DP)
Laboratory Guidelines for Animal Care
Differentiate ES cells into cystic embryoid bodies
Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo
Apoptosis: Miniassay
Senescence Regulation by the p53 Protein Family
Molecular Profiling of the Epidermis: A Proteomics Approach
Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies
Phenotypic Characterization of Parp-1 and Parp-2 Deficient Mice and Cells
Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells
Screening for Inhibitors of Microtubule-Associated Motor Proteins
Pathways Implicated in Stem Cell Migration: The SDF-1/CXCR4 Axis
球体细胞培养
Interactions of Amphipathic CPPs with Model Membranes
选择最适合的细胞增殖检测方法
Functionality Screen of Streptavidin Mutants by Non-Denaturing SDSPAGE Using Biotin-4-Fluorescein
细胞的分裂指数
Evaluation of Tankyrase Inhibition in Whole Cells
提高U251的转染效率方法
Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting in
HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminex Techno
Analysis of Meiotic Recombination in Caenorhabditis elegans
ChIP-Enriched in Silico Targets (ChEST), a ChIP-on-Chip Approach Applied to Analyzing Skeletal Muscl
Microspread Ovarian Cell Preparations for the Analysis of Meiotic Prophase Progression in Oocytes wi
In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduc
Lentiviral Vector-Mediated Expression of pre-miRNAs and AntagomiRs
Determination of Phosphoinositide Binding to K+ Channel Subunits Using a ProteinLipid Overlay Assay
Volatile Oxylipins and Related Compounds Formed Under Stress in Plants
Melanoblasts as Multipotent Cells in Murine Skin
Developmental Biology Protocols: Overview II
微管组织中心(microtubule organizing centers, MTOC)
相差观察和摄影
Use of Gene Overexpression to Assess Function in Cell Cycle Control
Using an Inverse PCR Strategy to Clone Large, Contiguous Genomic DNA Fragments
Cell Cycle Analysis of Asynchronous Populations
Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale
Methods for Determining the Proliferation of Cells in Response to EGFR Ligands
Splice Redirection as a Convenient Assay to Monitor CPPON Efficiency and Mechanism
Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue
The Reconstitution of Actin Polymerization on Liposomes
Measuring Angiogenesis in Mice
Peptidomic Analysis of Single Identified Neurons
圆球体(spherosome)
FISH Methods in Cytogenetic Studies
Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells
Sponge Implant Model of Angiogenesis
Microtubule Spindowns for Visual Analysis
The Measurement of Cyclic AMP Levels in Biological Preparations
Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches
Studying Mechanosensitive Ion Channels Using Liposomes
Genetic-Engineered Models of Skeletal Diseases II: Targeting Mutations into Transgenic Mice Chondroc
血影蛋白(spectrin)
PACE Analysis of RNA-Peptide Interactions
分泌（secretion）
Generation and Characterization of Epiblast Stem Cells from Blastocyst-Stage Mouse Embryos
Goat Embryonic Stem-Like Cell Derivation and Characterization
Measurement of Autophagy in Cells and Tissues
Quantification of Redox Conditions in the Nucleus
Methods for Characterization/Manipulation of Human Corneal Stem Cells and their Applications in Rege
Using Multiphoton Excitation to Explore the Murky Depths of Developing Embryos
Mobilization of Hematopoietic Stem/Progenitor Cells: General Principles and Molecular Mechanisms
The Role of Receptor Tyrosine Kinases in Primordial Germ Cell Migration
High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidases
Transdermal Delivery Using Surface Electrodes in Porcine Skin
hTERT Knockdown in Human Embryonic Kidney Cells Using Double-Stranded RNA
Application of Genetically Modified Feeder Cells for Culture of Keratinocytes
Cell Freezing
Membrane Protein Expression in Cell-Free Systems
乙醇酸途径(glycolate pathway)
Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes
Application of Antisense Oligodeoxynucleotides in Developing Chick Embryos
Detection of Meiotic DNA Breaks in Mouse Testicular Germ Cells
Identification, Assay, and Functional Analysis of the Antizyme Inhibitor Family
In Vivo Transplantation Assay at Limiting Dilution to Identify the Intrinsic Tissue Reconstitutive C
Primary Culture of Human Antral Endocrine and Epithelial Cells
Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations
Using the Yeast Genome-Wide Gene-Deletion Collection for Systematic Genetic Screens
Spectrofluorometric Assay for Cell-Tissue Electrofusion
Post-translational Control of ETS Transcription Factors: Detection of Modified Factors at Target Gen
Measurement of Plasma/Serum Acylcarnitines Using Tandem Mass Spectrometry
兔结缔组织生长因子（CTGF）酶联免疫分析（ELISA）
国外细胞株购买指南
Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus
Expression of an Integral Membrane Protein, the 5HT5A Receptor
Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells
Negative Stain Electron Microscopy of Microtubules
Assessment of Laterality Defects in Rodent Embryos
Steroid Hormone Metabolites and Hormone Binding Assays
Live to Dead Cell Imaging
Studies on Coagulation Incompatibilities for Xenotransplantation
无蛋白培养基与限定化学成分培养基的区别
From ES Cells to Mice: The Gene Trap Approach
Amplified Assay for Specific Dual-Labeled DNA Using the Coagulation Cascade (EDNA-ELCA)
Qualitative and Quantitative Analyses of Phospholipids by LCMS for Lipidomics
CO2培养箱的选购和使用技巧
Cultures of Adipose Precursor Cells and Cells of Clonal Lines from Animal White Adipose Tissue
Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives
hESC Engineering by Integrase-Mediated Chromosomal Targeting
Analyzing Planar Cell Polarity During Zebrafish Gastrulation
Analysis of Global Gene Expression Profiles Activated by Chemoattractant Receptors
Analysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cel
Viability Cell Count
Imaging Podosome Dynamics and Matrix Degradation
Isolation of Rat Telencephalic Neural Explants to Assay Shh GABAergic Interneuron Differentiation-In
Two-Photon Fluorescence Imaging and Reactive Oxygen Species Detection Within the Epidermis
Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery
A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in
Analysis of Fiber-Type Differences in Reporter Gene Expression of -Gal Transgenic Muscle
Enhanced Immunohistochemical Resolution of Claudin Proteins in Glycolmethacrylate-Embedded Tissue Bi
食蟹猴白细胞介素-6（IL-6）酶联免疫分析（ELISA）
二羧酸转运载体(dicarboxylate exchange carrier)
Retroviral Transduction of Murine Primary T Lymphocytes
High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450
Lipid Profiling Using Two-Dimensional Heteronuclear Single Quantum Coherence NMR
烟酰胺乙酰胆碱受体在凋亡中的作用通路图
电子载体(electron carriers)
Knock-in and Knock-out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways
大鼠路氏锥虫IgG抗体酶联免疫分析（ELISA）
Culturing Mouse Embryonic Fibroblasts
大鼠肌酸激酶(CK)酶联免疫分析（ELISA）
周期蛋白(cyclin)
Protein (Western) Blotting with Immunodetection
The Role of the Adapter Protein SHB in Embryonic Stem Cell Differentiation Into the Pancreatic -Cell
细胞生物基本方法：上皮细胞培养
Direct tissue explant method for derivation and study of extravillous
腹腔巨噬细胞吞噬功能检测方法
Stock solutions for tissue culture
体细胞Barr小体观察
Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA
Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL
Electron Microscopic Immunogold Localization of Recombination-Related Proteins in Spreads of Synapto
Immunohistochemistry and Detection of Proliferating Cells by BrdU
High-Throughput Screens to Discover Synthetic Riboswitches
Isolation and Characterization of Human Fetal Myoblasts
Hypoxic Gene Regulation in Differentiating ES Cells
Time-Lapse Epifluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oo
基因的连锁与交换
转染细胞不表达怎么补救
Force Generation by Dynamic Microtubules In Vitro
Directing Epidermal Fate Selection by a Novel Co-Culture System
Xenotransplantation: An Overview of the Field
卵母细胞(oocyte)
Modulating and Targeting Meiotic Double-Strand Breaks in Saccharomyces cerevisiae
Use of Differentiating Embryonic Stem Cells in Pharmacological Studies
Protocols for Peptidomic Analysis of Spider Venoms
Invasion Assays and Matrix Metalloproteinases: Quantification of Cellular Invasion Using Propidium I
pc12培养经验
染色体(chromosome)
Umbilical Cord Blood Cells
Electron Microscopic Studies of Receptor Localization
Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters
Measurement of Low-Molecular-Weight Carboxylic Acids in Ambient Air and Vehicle Emission by Capillar
Mouse Tissue Culture Models of Unstable Triplet Repeats
Thin-Layer Chromatography of Phospholipids
Fluorescence In Situ Hybridization on Single Cells: Sex Determination and Chromosome Rearrangements
Release Testing of Retroviral Vectors and Gene-Modified Cells
A Cleanup Method for Mass Spectrometry of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a
裸鼠成瘤肿瘤长不出来怎么办?
做免疫组化时脱片怎么办？
免疫荧光结果背景强怎么办？
巨噬细胞纯化的方法有哪些？
流式分选和磁珠分选有什么区别？
免疫荧光定位不对怎么办？
什么是油红O染色？
油红O染色实验步骤与注意事项
【国自然热点文献】之乳酸化（三）
石蜡切片切不出蜡带怎么办？
什么是细胞瞬时转染？
什么是细胞稳定转染？
细胞稳定转染和瞬时转染有什么区别？
HE染色细胞核暗淡怎么办？
透射电镜看外泌体的哪些表征？
细胞瞬时转染和细胞稳定转染，两者的区别？
什么是质粒
质粒抽提的详细实验步骤是怎样的？
SNP变异后为什么有两种碱基？
单细胞悬液有什么作用？
做组织切片为什要固定？如何固定？
石蜡包埋时要注意什么？
双安荧光素酶转染率低怎么办？
做COIP没有想要的目的条带怎么办？
为什么PAS染色很淡？具体原因及解决方法
免疫荧光信号不真实的原因及解决办法
western blot检测实验常见问题及解决方法
tunel染色结果怎么分析，每个结果的异常原因及调整方法
动物造模血管功能实验步骤
原代细胞转染技巧
细胞焦亡检测金标准
常见的细胞实验有哪些
cck8检测原理和实验步骤
细胞诱导分化是怎样的？
质粒提取的方法以及原理和常见问题
番红固绿染色原理，实验步骤以及需要注意什么？
什么是FISH技术？
FISH染色实验步骤
WB实验原理及流程图
WB实验检测介绍——检测小鼠组织中GSTM2的表达
实时荧光定量 PCR（qPCR）的介绍
实时荧光定量pcr的计算
一看就会的油红O染色实验指南
为什么质粒转染荧光检测会失败？
质粒转染荧光检测失败后的解决办法
做酶联免疫吸附剂测定实验需要注意什么？
关于SNP检测流程和应用
双荧光素酶报告测定的方法
双荧光素酶检测实验步骤
双荧光素酶检测结果是怎么分析的？
什么是CHIP技术？——CHIP技术原理
CHIP实验步骤
GST pulldown实验步骤
GST pulldown实验的需要注意什么？
GST pulldown实验结果分析
酵母双杂交的实验原理和步骤
酵母双杂交实验采用的系统有哪些？
酵母双杂交的实验设计是怎么样的？
酵母双杂交实验技术的作用
RIP检测实验流程
RIP实验设计方案是怎样的？
冰冻切片和石蜡切片有所不同？
怎样进行免疫共沉淀实验的分析？
免疫共沉淀—实验操作流程和步骤
免疫共沉淀（Co-IP）实验技术
GST-puiidown和coip有何区别？
RNA pull-down实验原理介绍+实验步骤
RNA pull-downll实验结果怎么看？
RNA pull-downll实验结果分析
什么是 northern blot技术？
northern blot实验详细操作报告
northern blot实验作用和原理
southern blot实验目的是什么？
southern blot实验流程
southern blot原理和应用
EMSA实验概念和原理
EMSA实验的步骤
在进行EMSA实验为什么看不到迁移带？
EMSA实验结果要怎么看？
EMSA测定需要多少量的蛋白标记的探针？
EMSA实验过程膜没有一直处于湿润状态怎么办？
Dot Blot实验方法以及操作步骤
Dot Blot实验结果分析报告是怎样的？
Dot Blot实验和western blot实验有何区别？
为什么细胞会衰老？主要原因有哪些？
细胞衰老的主要特征
血管生成的基本过程是怎样的？
血管生成的原因和方式
血管生成的相关实验和疾病有哪些？
血管形成实验操作步骤
血管生成的指标
常做的细胞实验有哪些？
肿瘤细胞成球实验
细胞焦亡的机制
常用的细胞焦亡检测方法包括？
细胞焦亡是程序性死亡吗？
细胞早期凋亡和晚期凋亡的区别？
细胞凋亡和焦亡坏死的区别？
免疫表型检测的目的
免疫表型是什么？
免疫表型检测报告怎么看？
细胞融合与培养细胞的形态观察和计数
猴B病毒抗体（BV-Ab）酶联免疫分析（ELISA）
CELL CYCLE ANALYSIS
Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents for Studying Membrane Proteins
Immune Ablation Followed by Autologous Hematopoietic Stem Cell Transplantation for the Treatment of
肌钙蛋白(troponin，Tn)
In Vitro Analyses of Endothelial Cell Permeability
Animal Models of Multiple Sclerosis
中期(metaphase)
The CE Way of Thinking: All is Relative!
Multilocus Enzyme Electrophoresis for Parasites and Other Pathogens
MMPs and TIMPs: An Historical Perspective
Chemotaxis of Slow Migrating Mammalian Cells Analysed by Video Microscopy
Cell Arrays for the Measurement of Organelle Dynamics in Living Cells
Detection and Quantification of Cocaine and Benzoylecgonine in Meconium Using Solid Phase Extraction
Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption
Serial Analysis of Gene Expression in Human Keratinocytes and Epidermis
Apoptosis in the Epidermis
细胞培养中的“黑胶虫”问题
CDC25 Dual-Specificity Protein Phosphatases: Detection and Activity Measurements
细胞遗传学――原位引物启动技术(PRINS)
Blue Native-Gel Electrophoresis Proteomics
Construction and Purification of Adhesion Molecule Immunoglobulin Chimeric Proteins
In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells
Parallel Detection of Crossovers and Noncrossovers in Mouse Germ Cells
人胸腺基质淋巴细胞生成素 (TSLP)酶联免疫分析（ELISA）
Differentiation and Characterization of Human MSCs
Studying Gene Expression in Bone by In Situ Hybridization
RNA Footprinting and Modification Interference Analysis
Analytic Methods for Modeling Stochastic Regulatory Networks
The Double Immunodiffusion Technique: Immunoprecipitation and Analysis of Antigenic Protein in Gel
Cupric Ion Reducing Antioxidant Capacity Assay for Food Antioxidants: Vitamins, Polyphenolics, and F
A Novel Experimental Platform for Investigating Cancer Growth and Anti-Cancer Therapy in a Human Tis
Assay to Measure Oxidized and Reduced Forms of CoQ by LCMS/MS
A Sensitive and Versatile Cytokine Bioassay Based on Type I Interferon Signaling in 2fTGH Cells
Electroporation of Adherent Cells In Situ for the Introduction of Nonpermeant Molecules
Affinity Peptidomics: Peptide Selection and Affinity Capture on Hydrogels and Microarrays
Use of Liposomes Containing Carbohydrates for Production of Monoclonal Antibodies
The chick embryo is easily accessible and has therefore been widely used in developmental biology st
Time-Lapse Imaging of Chick Cardiac Precursor Cells
Quantitative Analysis of Distal Tip Cell Migration in C. elegans
Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens
Immunocytochemistry to Study Myogenesis in Zebrafish
Reversible Covalent Immobilization of Enzymes via Disulfide Bonds
Gene Mapping to Chromosomes by Hybridization in Situ
大鼠细小病毒抗体(RPV)酶联免疫分析
A New Approach to Primary Culture of Human Gastric Epithelium
TAT-Peptide Modified Liposomes: Preparation, Characterization, and Cellular Interaction
Production and Purification of Monoclonal Antibodies
Monitoring and Analyzing Drosophila Circadian Locomotor Activity
Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos
Isolation and Culture of Human Osteoblasts
Detection of Cell Death by Autophagy
Analysis of Rhythmic Gene Expression in Adult Drosophila Using the Firefly Luciferase Reporter Gene
大鼠apelin (AP)酶联免疫分析（ELISA）
Expression and Analysis of Exogenous Proteins in Epidermal Cells
Ribonuclease Inhibitors
囊胚（blastula）
小鼠核因子kb受体活化因子配基（RANKL）酶联免疫分析
Methods to Study MYC-Regulated Cellular Senescence
Isolation of cell nuclei for the application in the cell-free system
无血清培养基与完全培养基体外诱导扩增 CIK细胞
巨噬细胞原代培养
数量性状的统计分析实验
TUNEL assay
人白细胞介素-18（IL-18）酶联免疫分析（ELISA）
Flow Sorting Fibroblasts with GFP and P.I.
Sequencing PCR Products Cloned into M13 Vectors
基质(ground substance)
大批量生产干细胞培养基的具体细节
Proteolytic Enzymes for Peptide Production
Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts
Assay for Neuronal Cell Migration
配体(ligand)
细胞介导细胞毒作用检测法5：用MTT检测法测定CMC
大鼠免疫球蛋白E(IgE)酶联免疫分析（ELISA）
Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse Microscopy
微球体体系
Preparation of Myristoylated Arf1 and Arf6 Proteins
In Situ Localization of PCR-Amplified DNA and cDNA
Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
Differential Expression of TIMP-3 During Neoplastic Progression in the Mouse JB6 Model System
Human Embryogenesis: Overview
Electrophoresis of Cerebrospinal Fluid
胞质环流(cytoplasmic streamting)
TGF- Signaling in Embryonic Stem Cell-Derived Endothelial Cells
Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes
Human Hepatocytes: Isolation, Culture, and Quality Procedures
Transcription Factor Sequestration by Polyglutamine Proteins
Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells
BiotinProtein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applica
Biocompatible Nanoparticle Labeling of Stem Cells and Their Distribution in Brain
免疫表型研究分析
CCK8检测实验报告和详细的实验步骤
CCK-8实验基本步骤以及注意事项
CCK8细胞增殖率怎么计算分析？
CCK8检测细胞增殖的目的以及方法
mtt实验与CCK8实验的区别？
细胞划痕实验原理以及步骤
细胞划痕用什么拍照？
细胞划痕实验说明什么？
细胞划痕实验是怎么分析的？
细胞划痕实验结果计算公式
在进行细胞划痕实验中有什么技巧？
什么是细胞侵袭实验？以及该实验前的准备
细胞侵袭实验步骤
细胞侵袭实验结果怎么读？
轻松鉴别细胞侵袭和迁移
单细胞多组学完整解决方案
细胞侵袭实验常见问题以及解决办法
在细胞实验中还有哪些常见的细胞侵袭实验？
激光共聚焦的优点是什么？以及具体的实验流程
植物各器官冰冻切片最适宜温度是多少？
什么是克隆形成？
克隆形成实验报告和实验操作步骤
克隆形成实验操作注意事项
克隆形成有哪些分类和优点？
原代细胞分离培养与鉴定有哪些优点？
In Vitro Culture of Fetal Ovaries: A Model to Study Factors Regulating Early Follicular Development
Claudins in a Primary Cultured Puffer Fish (Tetraodon nigroviridis) Gill Epithelium
SAMe and HuR in Liver Physiology: Usefulness of Stem Cells in Hepatic Differentiation Research
Microarray Technology for Mutation Analysis of Low-Template DNA Samples
B-Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization
Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and Martigel Gels
High-Throughput Screening of Microtubule-Interacting Drugs
Screen for Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase
流式细胞仪在睡眠性阵发性血红蛋白尿的诊断中的应用
Counting Chromosomes in Intact Eggs
Viral Transformation of Epithelial Cells
The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of P
Genome-Wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq
Determination of Proteasomal Activities
Sequencing-Based Typing of HLA
Tumor Growth Fraction Estimation, Perturbation, and Prognostication
Methods for Studying Spindle Assembly and Chromosome Condensation in Xenopus Egg Extracts
Receptor Knock-Out and Knock-In Strategies
Pre-embedding Nanogold Silver and Gold Intensification
Purification of Native Bone Marrow Megakaryocytes for Studies of Gene Expression
Correlative Transmission Microscopy: Cytochemical Localization and Immunocytochemical Localization i
Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard
Lysosome Isolation in Isotonic Sucrose
Assessment of LiposomeCell Interactions
Phenotypic Analysis and Differentiation of Murine Mesenchymal Stem Cells
通过细胞融合得到B细胞杂交瘤实验
viral immunity pathogenesis group
Identification and Analysis of Epidermal Stem Cells from Primary Mouse Keratinocytes
The Isolation and Culture of Human Cord Blood-Derived Mesenchymal Stem Cells Under Low Oxygen Condit
Intravital Microscopy to Study Leukocyte Recruitment In Vivo
Generation of a Tetracycline Regulated Mouse Model of MYC-Induced T-Cell Acute Lymphoblastic Leukemi
High-Resolution Capillary Gas Chromatography in Combination with Mass Spectrometry for Quantificatio
Diagnosis of Creatine Metabolism Disorders by Determining Creatine and Guanidinoacetate in Plasma an
Neonatal Testicular Gonocytes Isolation and Processing for Immunocytochemical Analysis
原代细胞和传统细胞培养的区别
Isolation of Lipid Rafts From B Lymphocytes
原代细胞培养失败的原因
Cell Senescence as Both a Dynamic and a Static Phenotype
原代细胞培养注意事项
原代细胞分离培养与鉴定有哪些优点？
生物实验设计原则及需警惕的科研逻辑谬误
什么是EDU检测细胞增殖？
EdU检测实验操作步骤——及荧光定量分析数据
EDU检测细胞增殖结果如何分析？
了解细胞增殖的新视角 —— 检测EDU技术的方法
细胞增殖检测之EdU法及其应用
什么是外泌体提取？
外泌体提取的具体方法及步骤
如何正确提取外泌体
外泌体提取实验常见方法以及优缺点
如何构建和生成耐药细胞系？
什么是shRNA载体构建？
shRNA载体构建有哪些不同类型？
shRNA载体构建实验的详细过程
shRNA载体构建的优缺点
shRNA载体构建注意事项
介绍CRISPR/Cas9载体构建
CRISPR/Cas9载体构建的详细步骤和过程
CRISPR/Cas9载体构建技术要点及流程
CRISPR/Cas9载体构建技术特点与应用
CRISPRCas9载体构建相关问题和注意事项
分子克隆的定义
分子克隆：从基本概念到实验流程
解锁核孔蛋白NUP210的力量：通过破坏肿瘤细胞的力学响应，抑制转移
分子克隆里基因到蛋白质的五个关键步骤
分子克隆的方法有哪些？
分子克隆注意事项及应用实例分析
分子克隆和亚克隆的区别？
定点突变的定义
了解PCR定点突变的原理
定点突变技术方法有哪些？
定点突变实验和定向突变的区别
PCR定点突变实验遇到的问题和解决办法
什么是质粒提取？
质粒提取平衡的原理
质粒提取原理及操作步骤
质粒提取和纯化的原理
质粒提取常见问题以及解答
质粒提取的目的
慢病毒包装的原理
慢病毒包装系统介绍
慢病毒包装系统应用和步骤
慢病毒包装常见问题及解决方案
慢病毒包装的注意事项
裸鼠皮下成瘤实验技巧和方法
Isolation of Plant Mitochondrial RNA from Green Leaves
Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embry
Human Myoblasts and Muscle-Derived SP Cells
In Situ Detection of microRNAs in Animals
小鼠骨髓细胞染色体标本制作[细胞遗传]
Gene Silencing for Functional Analysis: Assessing RNAi as a Tool for Manipulation of Gene Expression
A Review of Post-translational Modifications and Subcellular Localization of Ets Transcription Facto
Statistical Topics in the Laboratory Sciences
Investigating Gene Expression: In Situ Hybridization and Reporter Genes
Ribosomal Initiation Complexes Probed by Toeprinting and Effect of trans-Acting Translational Regula
Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis
Electroporation of ES cells
Refolding of TIMP-2 from Escherichia coli Inclusion Bodies
Human Embryo Culture Media Comparisons
Preparation of Intact, Highly Purified Phagosomes from Dictyostelium
Solid Tissue Dispersal for Cytokinetic Analyses
RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues
Transient Expression of Epitope-Tagged Proteins in Mammalian Cells
Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)
染色体CBG标本的制备
Urothelial Cell Culture: Stratified Urothelial Sheet and Three-Dimensional Growth of Urothelial Stru
In Situ Biosynthesis of Peptide Arrays
细胞培养染色体显示
Physical Properties of the Nucleus Studied by Micropipette Aspiration
Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections
李斯特氏菌在细胞间传播方式
Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes
流式样本的固定和保存
Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture
Analysis of Gene-Specific and Genome-Wide Sperm DNA Methylation
关于乳鼠心肌细胞培养的一点收获
Quantitation of Tacrolimus in Whole Blood Using High Performance Liquid Chromatography-Tandem Mass S
In Vivo Imaging of Oligonucleotidic Aptamers
Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes
Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity
Neural Transplantation and Stem Cells
Determination of Gene Expression Patterns by In Situ Hybridization in Sections
Gene Modification with Hapaxoterministic Restriction Enzymes: Easing the Way
Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix
Manipulating the Avian Epiblast and Epiblast-Derived Stem Cells
Isolating Aptamers Using Capillary ElectrophoresisSELEX (CESELEX)
Expansion of Pluripotent Stem Cells in Defined, Xeno-Free Culture System
Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells
配子或终末减数分裂（Gametic or terminal meiosis）
暗反应(dark reaction)
Ultrasound Backscatter Microscopy of Mouse Embryos
Biosensor-Based Evaluation of Liposomal Binding Behavior
Semiquantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Motoneur
Kinetic Analysis of the Inhibition of Matrix Metalloproteinases: Lessons from the Study of Tissue In
The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells
Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis
Screens for Mitochondrial Mutants in Yeast
A number of different viruses interact with the cell cycle in order to subvert host-cell function an
The Cell Cycle and Virus Infection
The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference
斑块连接 (plaque-bearing junction)
组织培养细胞染色体制备方法
In vitro culture of embryonic lungs
In Vitro Generation of Lymphocytes From Embryonic Stem Cells
$冰冻断裂复型(freeze-fracture replication)技术$
冰冻断裂复型(freeze-fracture replication)技术
Enzyme Immobilization on Polyethyleneimine-Coated Magnetite Particles
Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors
Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abun
The Application of Nuclear Magnetic Resonance to Structural Studies of Polysaccharides
裸鼠皮下成瘤实验常见操作问题以及解决办法（下）
如何巧妙设计引物？
设计引物的原则
qPCR引物设计流程
引物设计的依据
PCR设计引物的具体步骤
设计引物中有哪些小技巧？
PCR引物设计以及PCR实验细节详解
酶切位点是什么？
酶切位点是如何确定的？
Mesenchymal Stem Cell Assays and Applications
Light Microscopy in Aquatic Ecology: Methods for Plankton Communities Studies
亲和标记(affinity labeling)
非组蛋白(nonhistone proteins)
The C. elegans Cell Cycle: Overview of Molecules and Mechanisms
Ex Vivo Expansion of Human Mesenchymal Stem Cells on Microcarriers
Methods for Measuring Ligand Dissociation and Nuclear Receptor Turnover in Whole Cells
Synchronization of Medicago sativa Cell Suspension Culture
RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells
如何使昆虫细胞适应新的培养液
Studying Drug-Tubulin Interactions by X-Ray Crystallography
Recommended Experiments with Isolated Mitochondria
$Cuticle Refraction Microscopy: A Rapid and Simple Method for Imaging Drosophila Wing Topography, an$
Cuticle Refraction Microscopy: A Rapid and Simple Method for Imaging Drosophila Wing Topography, an
Polyamine Analysis by LC-MS
Methods for Studying the Nuclei and Chromosomes of Dinoflagellates
Human Endothelial and Osteoblast Co-cultures on 3D Biomaterials
Electron Microscopy and Lac-Z Labeling
RaPID: An Aptamer-Based mRNA Affinity Purification Technique for the Identification of RNA and Prote
细胞增殖的酸性磷酸酶检验法操作步骤
Immunofluorescence Staining of Human Cells by Lysed Whole Blood
Poly(ADP-Ribose) Metabolism Analysis in the Nematode Caenorhabditis elegans
Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes
Live Analysis of the Division Cycles in X-Irradiated Drosophila Embryos
Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Su
Natural Killer Cells and Killer-Cell Immunoglobulin-Like Receptor Polymorphisms: Their Role in Hemat
孢子或中间减数分裂（Sporic or intermediate meiosis）
Use of Intact Xenopus Oocytes in Nucleocytoplasmic Transport Studies
Directed Differentiation and Characterization of Genetically Modified Embryonic Stem Cells for Thera
个体发育(individual development)
Functional Assays for Human Embryonic Stem Cell Pluripotency
Comparative Physical Mapping: Universal Overgo Hybridization Probe Design and BAC Library Hybridizat
Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules: Selective Protocols of Immunization
Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microsc
Small Vectors for Expression Based on Dominant Drug Resistance with Direct Multicopy Selection
Small Animal Models of Xenotransplantation
RNA In Situ Hybridizations on Drosophila Whole Mounts
The Application of Adenoviral Vectors in the Study of Mammalian Cardiovascular Development
Assessment of Permeability in Barrier Type of Endothelium in Brain Using Tracers: Evans Blue, Sodium
肿瘤细胞的软琼脂集落培养和测定
Immunofluorescent Microscopic Study of Meiosis in Zebrafish
Flow Cytokinetics
Identification of Vertebrate Circadian Clock-Regulated Genes by Differential Display
A Quantitative Assay for Measurement of Chemokinesis in Tetrahymena
Ultrasonic Manipulation of Single Cells
Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae
Isolation of Embryonic Hematopoietic Niche Cells by Flow Cytometry and Laser Capture Microdissection
DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
High-Resolution Light Microscopic Characterization of Spermatogonia
Mammalian Target of Rapamycin: A Signaling Kinase for Every Aspect of Cellular Life
Laser Ablation and Fate Mapping
Isolation and Cultivation of Human Scalp Interfollicular Epidermal Stem Cells
Media Composition: Macromolecules and Embryo Growth
大鼠白细胞介素10（IL-10）酶联免疫分析
Genome-Wide Mapping of Meiotic DNA Double-Strand Breaks in Saccharomyces cerevisiae
Nuclear Envelope Formation In Vitro: A Sea Urchin Egg Cell-Free System
DNA Transposons for Modification of Human Primary T Lymphocytes
A Direct-Sequencing-Based Strategy for Identifying and Cloning cDNAs from Differential Display Gels
Transcriptome Analysis of Common Gene Expression in Human Mesenchymal Stem Cells Derived from Four D
Activation of Oocytes After Nuclear Transfer
Application of Sonic Hedgehog to the Developing Chick Limb
Human Thymic Epithelial Cell Cultures
Construction of Radiation Hybrid Panels
Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells
Assessment of Nanomaterials Cytotoxicity and Internalization
Optimization of the Caco-2 Permeability Assay to Screen Drug Compounds for Intestinal Absorption and
愈伤组织的诱导
Establishment and Maintenance of Normal Human Keratinocyte Cultures
同步化(synchronization)
Single Nucleotide Polymorphism Genotyping in MMP Genes: The 5 Nuclease Assay
Proteins Involved in Cell Migration from Glioblastoma Neurospheres Analyzed by Overexpression and si
Treatment of Mice with Retinoids In Vivo and In Vitro: Skeletal Staining
Electrofusion of Mammalian Oocytes and Embryonic Cells
Analysis of Bacterial Proteins by 2DE
Electroporation for Gene Therapy
Human Long-Term Bone Marrow Culture
Morphometric Evaluation of the Spermatogonial Stem Cell Distribution and Niche in Vertebrates
The Use of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embr
Intrathymic Injection for Analysis of T-Cell Progenitor Activity
Long-Term Culture-Initiating Cell Assays for Human and Murine Cells
Analysis of Phosphoinositide Dynamics During Phagocytosis Using Genetically Encoded Fluorescent Bios
High-Throughput Screens Based on NAD(P)H Depletion
Monitoring Rapid Endocytosis in the Electron Microscope via Photoconversion of Vesicles Fluorescentl
细胞转染操作步骤
Probing RNA-Protein Interactions by Psoralen Photocrosslinking
Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction
Peptidomics: Identification of Pathogenic and Marker Peptides
Application of PepFect Peptides for the Delivery of Splice-Correcting Oligonucleotides
Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver
内含信号序列(internal signal sequence)
In Situ Hybridization Using cRNA Probes: Isotopic and Nonisotopic Detection Methods
Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Proc
ENU-Based Phenotype-Driven Screening
Image Analysis of Chromatin Remodelling
Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes
Activation of p53 Protein Function in Response to Cellular Irradiation
Cryopreservation of Mouse Embryos
Isolation of Messenger RNA
Using High-Content Microscopy to Study Gonadotrophin-Releasing Hormone Regulation of ERK
Measuring the Inflammasome
Determination of Total Homocysteine in Plasma Using Liquid Chromatography Coupled to Tandem Mass Spe
Animal Models of Neurodegenerative Diseases
Detection of an mRNA Polymorphism by Differential Display
Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes
Physical Structure of Nuclear Receptor-DNA Complexes
E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression
酶切位点的详细步骤
怎么看质粒上的酶切位点？
酶切位点怎么设计？
酶切位点与识别位点有区别吗？
常见酶切位点以及保护碱基
酶切位点怎么判断？
酶切位点序列表
免疫荧光检测是什么？
免疫荧光检测原理
免疫荧光染色操作步骤以及目的——细胞篇
免疫荧光染色操作步骤以及目的
免疫荧光结果判读
免疫荧光和elisa的区别是什么？
什么是western blot？
WB检测实验报告和详细的操作步骤（中）
Purification of SR Protein Splicing Factors
Engineering of Implantable Liver Tissues
细胞表面抗原染色的组织样本降解表
Cardiac Morphogenesis and Dysmorphogenesis: An Immunohistochemical Approach
Tandem Mass Spectrometry
Purification Techniques of Subcellular Compartments for Analytical and Preparative Purposes
Single Molecule Imaging of RNA In Situ
Preparation of Mitochondria from Rat Liver
Analysis of TGF-Inducible Apoptosis
Liposomes: Applications in Protein-Lipid Interaction Studies
252-Californium Plasma Desorption Time-of-Flight Mass Spectrometry of Peptides and Proteins
Biomedical Applications of Immobilized Enzymes: An Update
Tools for Predicting Binding and Insertion of CPPs into Lipid Bilayers
Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Lay
支原体污染的不同处理方法对比
The Use of Dansyl-Calmodulin to Study Interactions with Channels and Other Proteins
Platelet Shape Change and Spreading
Studying Mycs Role in Metabolism Regulation
Respiratory and Thermogenic Capacities of Cells and Mitochondria from Brown and White Adipose Tissue
Morphometric Analysis of Centrosome Position in Tissues
Transduction of Murine Embryonic Stem Cells by Magnetic Nanoparticle-Assisted Lentiviral Gene Transf
Media Composition: Antioxidants/Chelators and Cellular Function
Using Cells Encapsulated in Agarose Microbeads to Analyse Nuclear Structure and Functions
Enzyme Analysis: Cathepsin D as an Example
Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry
Regulation of Apoptosis by the Unfolded Protein Response
Utility of Antioncogene Ribozymes and Antisense Oligonucleotides in Reversing Drug Resistance
Ex Vivo Generation of Human Red Blood Cells: A New Advance in Stem Cell Engineering
Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton
内体(endosome)
Assessment of Myocardial Angiogenesis and Vascularity in Small Animal Models
Real-Time PCR Quantification of Gene Expression in Embryonic Mouse Tissue
Atomic Force Microscopy for the Characterization of Proteoliposomes
Nuclear Transplantation and Cloning in Mammals
Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level
A High-Throughput siRNA Screening Platform to Identify MYC-Synthetic Lethal Genes as Candidate Thera
Small-Molecule Collection and High-Throughput Colorimetric Assay to Identify PARP1 Inhibitors
组成型分泌途径(constitutive secretory pathway)
Detection of NF-B Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis
Methods for Iodination of Active TGF, TGF Receptor Crosslinking and Immunoprecipitation of TGF-Recep
Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging
Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endot
Covisualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3D DNA Methylation
Methods for Analysis of Ligand-Induced RNA Conformational Changes
Sources and Derivation of Human Embryonic Stem Cells
Master Cell Bank
PNA-In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent D
Recording Ion Channels in Isolated, Split-Opened Tubules
Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test
Tiny Molecular Beacons for in vivo mRNA Detection
Saponin Lysis of RBCs
Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders
Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO
Wnt Signaling During Caenorhabditis elegans Embryonic Development
A combination of forward and reverse genetic approaches in zebrafish has revealed novel roles for ca
Wnt Signaling Mediates Diverse Developmental Processes in Zebrafish
人NK 细胞克隆的制备
Fluorescence Correlation Spectroscopy to Assess the Mobility of Nuclear Proteins
Utilization of Liposomes for Studying Drug Transfer and Uptake
染色体结构显示与检测
Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins: Part A. Preparation
Application of Laser Microdissection to Study PlantFungal Pathogen Interactions
Enzyme Immobilization by Entrapment Within a Gel Network
In Vitro Selection of Random RNA Fragments to Identify Protein-Binding Sites Within Large RNAs
Distribution of Bleomycin in a Rat Model
Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates
Small Molecule Screen in Zebrafish and HSC Expansion
The US and EU Regulatory Perspectives on the Clinical Use of Hematopoietic Stem/Progenitor Cells Gen
Routes of Stem Cell Administration in the Adult Rodent
Removing cells from liquid nitrogen
WB检测实验报告和详细的操作步骤（中）
WB检测实验报告和详细的操作步骤（下）
western blot为什么必须要用内参?
western blot条带如何看？
western blot内参怎么看？
western blot仪器介绍
western blot一抗和二抗是什么？
QPCR检测的是什么？
QPCR检测实验详细的步骤与报告（三）
QPCR原理以及数据处理
QPCR原理和主要的三个步骤
QPCR和PCR区别点和共同点是什么？
QPCR内参是什么？
QPCR实验设计
QPCR数据分析以及作图方法
核质分离实验QPCR数据处理
QPCR目的和意义
流式检测的原理以及应用
流式检测实验报告——细胞周期检测
流式细胞检测实验步骤总结
流式检测细胞周期怎么分析？
流式检测ros结果分析
流式细胞检测报告概述
流式细胞检测那些项目？
流式检测阴行对照意义
活体成像的原理
活体成像技术
小鼠活体成像的实验步骤
活体成像荧光强度定量
活体成像的注意事项
HE染色原理和意义
HE检测实验报告（一）
HE检测实验报告（二）
HE检测实验报告（三）
HE检测实验报告（四）
HE检测实验报告（五）
HE染色实验操作标准
HE染色结果分析
HE染色常见问题分析和解决方法
HE染色和免疫组化的区别是什么？
为什么HE染色切片着色浅？
he染色中出现切片或染液、组织及盖载玻片的污染怎么办？
HE染色过程中切片内有大小不一的气泡怎么办
Transwell细胞迁移
Transwell共培养
Transwell检测实验报告
Transwell迁移实验报告
Transwell侵袭实验报告
Transwell诱导单层细胞屏障
什么是Transwell转运率？
Transwell细胞计数的原理
Transwell迁移和侵袭的区别
Transwell细胞培养分选
Transwell细胞迁移实验注意事项
Transwell侵袭注意事项
什么是原位杂交实验？
荧光原位杂交检测实验报告（三）
原位杂交检测实验报告（四）
原位杂交目的
原位杂交实验分析
原位杂交实验的应用
原位杂交实验操作细节有哪些？
原位杂交常见问题的分析与对策
原位杂交方法
什么是激光共聚焦技术？
激光共聚焦实验步骤详解
激光共聚焦结果分析
激光共聚焦样品制备方法
激光共聚焦实验原理、应用以及发展是什么？
激光共聚焦实验目的
激光共聚焦和荧光共聚焦的区别
ELISA原理、操作以及注意事项
ELISA酶联免疫吸附试验报告
ELISA酶联免疫吸附试验结果分析
ELISA酶联免疫吸附试验实验目的
ELISA实验方法有哪些？
ELISA实验步骤
ELISA酶联免疫吸附试验常见问题以及解决
什么是小鼠肠炎模型？
小鼠肠炎模型构建实验报告展示
小鼠结肠炎诱导方法
小鼠肠炎模型周龄怎么算？
小鼠肠炎模型体温怎么测量？
小鼠肠炎模型对小鼠品种有什么要求吗？
小鼠肠炎模型注意事项
小鼠肠炎模型实验过程中问题和解决方法
EMSA凝胶迁移实验原理
EMSA凝胶迁移实验分类
EMSA凝胶迁移实验步骤
EMSA凝胶迁移实验电泳图分析
EMSA凝胶迁移实验结果分析
EMSA凝胶迁移实验目的
EMSA凝胶迁移实验注意事项
EMSA凝胶迁移蛋白跑不下来是什么原因？
EMSA凝胶迁移实验里BSA的作用是什么？
EMSA凝胶迁移实验过程中常见问题与解答
TUNEL染色原理介绍【组织染色】
TUNEL染色实验原理和实验操作技巧
TUNEL检测实验报告（一）
TUNEL检测实验报告（二）
TUNEL检测实验报告（三）
如何进行TUNEL染色阳性对照实验？
如何正确解读TUNEL染色结果图？
TUNEL染色实验试剂选择和配置方法
TUNEL染色和DAPI染色区别
TUNEL染色和HE染色区别是什么？
Tunnel染色实验注意事项
TUNEL染色实验在凋亡检测中的优势与局限性
TUNEL染色实验与细胞凋亡机制
TUNEL染色实验与荧光显微镜
TUNEL染色实验中的质量控制措施
TUNEL染色实验过程中的常见问题与解决方法分析
深入了解MASSON染色：原理、步骤与实例
如何进行MASSON染色？
Masson染色实验报告分析
MASSON（马松）染色方法及其在实验室的应用
MASSON（马松）染色结果怎么分析的？
MASSON（马松）染色结果怎么看纤维化？
MASSON染色和HE染色区别?
MASSON（马松）染色原理阳性意义
Detection of Copy Number Variation Using SNP Genotyping
Isolation and Culture of Human Alveolar Epithelial Cells
Using Zebrafish (Danio rerio) to Assess Gene Function in Thrombus Formation
细胞生物基本方法：结缔组织类细胞培养
Treatment of Rat Glioma With Electrochemotherapy
紫杉醇(taxol)
原肌球蛋白(tropomyosin, Tm)
Proteomics Analysis of Contact-Initiated Eph ReceptorEphrin Signaling
Identifying and Counting Protein Modifications Triggered by Nitrosative Stress
Measurement and Illustration of Immune Interaction After Stem Cell Transplantation
Antisense Therapeutics: A Promise Waiting to Be Fulfilled
Metal Analysis With Capillary Zone Electrophoresis
Culture of Preimplantation Mouse Embryos
Derivation and Characterization of Thyrocyte-Like Cells From Embryonic Stem Cells In Vitro
Receptor-DNA Interactions: EMSA and Footprinting
细胞的冻存和复苏
Virus Vectors for Gene Therapy of the Nervous System
Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation
Electrotransformation of Chinese Hamster Ovary Cells
Nanoparticles/Dip Stick
Isolation of Stromal Stem Cells From Human Adipose Tissue
Clearance of Mutant Aggregate-Prone Proteins by Autophagy
Stable Transfection Using Episomal Vectors to Create Modified Human Embryonic Stem Cells
Two-Dimensional Electrophoresis: An Overview
Antibodies Against Huntingtin: Production and Screening of Monoclonals and Single-Chain Recombinant
Immunofluorescent Localization of Proteins in Caenorhabditis elegans Muscle
Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos
人白细胞介素23（IL-23）酶联免疫分析（ELISA）
上海市临床科研基本实验技能培训-细胞培养
MASSON（马松）染色实验目的
Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract
Analysis of Soluble Sugar Permease Domains by Solution NMR
Identification and Isolation of Adult Liver Stem/Progenitor Cells
Identification of Differentially Expressed Genes in Sorted Cell Populations by Two-Dimensional Gene-
Evaluating a Screen and Analysis of Mutant Libraries
Protein Complex Analysis of Native Brain Potassium Channels by Proteomics
Large and Small Scale RNA Preparations from Eukaryotic Cells
Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens
MASSON（马松）染色实验中染液的顺序
In Vitro Generation of T Lymphocytes From Embryonic Stem Cells
Differentiation of Human Embryonic Stem Cells Toward the Chondrogenic Lineage
Using RNA FISH to Study Gene Expression During Mammalian Meiosis
Synchronization of Pathogenic Protozoans
MASSON（马松）染色实验技术注意事项
Solubilization of Aggregates Formed by Expanded Polyglutamine Tract Expression in Cultured Cells
Sequencing and Phylogenomic Analysis of Whole Mitochondrial Genomes of Animals
MASSON（马松）染色实验过程中常见问题和解决方法
Improved Chemiluminescence Assay for Measuring Antioxidant Capacity of Seminal Plasma
Transmembrane Signaling by G Protein-Coupled Receptors
PAS染色实验原理介绍
Analysis of ERKs Dimerization by Electrophoresis
Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology
PAS检测实验报告【小鼠肾】
In Vitro Analysis of the Yeast Mitochondrial RNA Polymerase
Inhibition of DNA Synthesis by Ionizing Radiation
PAS染色实验报告【小鼠心肌】
Human and Murine Skeletal Muscle Reserve Cells
Real-Time Quantitative Polymerase Chain Reaction Measurement of Male Fetal DNA in Maternal Plasma
如何获得更清晰的染色效果？
Inducing High Rates of Targeted Mutagenesis in Zebrafish Using Zinc Finger Nucleases (ZFNs)
Transient transfection into 293T cells
PAS染色的实验步骤全攻略
PAS染色结果解读
Migratory Strategies of Normal and Malignant Stem Cells
Mesenchymal Stem Cell Therapy on Murine Model of Nonalcoholic Steatohepatitis
PAS染色实验材料有哪些？
螺旋管
Flow Cytometric Monitoring of Cellular Pharmacokinetics
PAS染色和mpo染色区别是什么？
Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex
Quantitation of Oxycodone in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)
Methods for the Study of Signaling Molecules in Membrane Lipid Rafts and Caveolae
PAS染色实验注意事项
Detection of Focal Proteolysis Using Texas-Red-Gelatin
联会复合体的染色与观察
细胞支原体污染的荧光检测方法[配图]
皮层反应(cortical reaction)
轴突运输(axonal transport)
Growing cells
Media Composition: pH and Buffers
PAS染色临床应用
PAS糖原染色常见问题及解决方案
CCK8实验原理
CCK8检测实验报告（一）
CCK8检测实验报告（二）
Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes
Analyses of ProteinProtein Interactions by In Vivo Photocrosslinking in Budding Yeast
Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Sta
Cloning by Limiting Dilution of Hybridoma
Study of Apoptosis In Vitro Using the Xenopus Egg Extract Reconstitution System
Derivation and Characterization of Neuronal Precursors and Dopaminergic Neurons From Human Embryonic
Procedures for Derivation and Characterisation of Human Embryonic Stem Cells from Odense, Denmark
Multicolor Whole-Mount In Situ Hybridization
Measurement of Cholesterol in Membranes
Grafting Hensens Node
Culture of Human Normal Brain and Malignant Brain Tumors for Cellular, Molecular, and Pharmacologica
Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling
Photoactivatable (Caged) Fluorescein as a Cell Tracer for Fate Mapping in the Zebrafish Embryo
F2-Isoprostanes: Sensitive Biomarkers of Oxidative Stress In Vitro and In Vivo: A Gas Chromatography
Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis
Poly(ADP-ribosyl)ation Pathways in Mammals: The Advantage of Murine PARG Null Mutation
Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
减数分裂压片标本制备和观察实验
Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Compe
Raman Spectroscopy for the Detection of AGEs/ALEs
Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic Cells
Analysis of Gene Expression Using cDNA Microarrays
Some Ethical, Social, and Legal Considerations of Xenotransplantation
巨噬细胞的分离与纯化实验方法
G418筛选实验常遇问题总结
Fluorescein labeling of proteins
FISH Banding Techniques
Blastocyst Culture for Deriving Human Embryonic Stem Cells
Assessing Cell Size and Cell Cycle Regulation in Cells with Altered TOR Activity
Modeling miRNA Regulation in Cancer Signaling Systems: miR-34a Regulation of the p53/Sirt1 Signaling
Aptamers Targeting RNA Molecules
Internal Standards in Differentiating Embryonic Stem Cells In Vitro
Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by Using Small Molecules
Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection
Isolation and Culture of Human Umbilical Vein Endothelial Cells
Nuclear Reprogramming by Cell Fusion
Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template
Isolation and Characterization of Human Prostate Stem/Progenitor Cells
DNA Microarray Studies of Hematopoietic Subpopulations
Analysis of Actin Assembly by In Vitro TIRF Microscopy
Detection of Adhesion Molecules by Immunohistochemistry on Human and Murine Tissue Sections
Identification of mRNA Bound to RNA Binding Proteins by Differential Display
Placental and Fetal Steroidogenesis
Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis
Strategies for the Development of Novel Taxol-Like Agents
Serial Orthotopic Transplantation of Epithelial Tumors in Single-Cell Suspension
Multiple Site-Directed Mutagenesis
Assessing PCP in the Cochlea of Mammalian Ciliopathy Models
蛋白质寻靶(protein targeting)
High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophi
染色体实验技术分析
Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells
Cryopreservation of Shoot-Tips and Meristems
Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells
Generation and Use of Epitope-Tagged Receptors
Cystic Fibrosis Airway Epithelial Cell Culture
Characterization of B-Cell Maturation in the Peripheral Immune System
Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET
Evaluating the Localization and DNA Binding Complexity of Histones in Mature Sperm
Slice Cultures for Study of Microglia
Production of Transgenic Mice with Yeast Artificial Chromosomes
Methods to Monitor Fatty Acid Transport Proceeding Through Vectorial Acylation
Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products
Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors
核仁(nucleolus)
Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures
Structural Probing Techniques on Natural Aptamers
Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and
CCK8实验技巧与改进方案
CCK8实验目的
CCK8实验数据分析
CCK8实验新手教程
CCK8实验在细胞增殖研究中的应用与优势
CCK8实验的图都有哪些？
cck8实验注意事项
CCK8实验的关键步骤与技巧
CCK8实验的过程中的问题剖析和解决方案
CCK-8实验公式
冰冻切片实验原理以及应用
冰冻切片实验步骤
冰冻切片实验结果分析是怎么样的？
冰冻切片实验目的
冰冻切片实验技巧分享
常用的冰冻切片实验仪器有哪些？
冰冻切片和石蜡切片的区别是什么？
冰冻切片实验常见问题以及解决方法
冰冻切片实验注意事项
什么是lncRNA？
lncRNA实验步骤
lncRNA引物设计的方法
如何根据基因检测lncRNA？
lncRNA实验教程
lncRNA实验意义
LncRNA简介以及应用
lncRNA技术分类
透射电镜的结构与成像原理探究
透射电镜实验样品制备技巧
透射电镜观察什么？
如何分析透射电镜实验数据？
Conjugation of Ligands to the Surface of Preformed Liposomes by Click Chemistry
Isolation and Culture of Pig Epiblast Stem Cells
停止转移肽(stop-transfer peptide)
光学显微镜的使用与细胞化学成分的分析
Analysis of Proteins by Capillary Electrophoresis
运输ATPase(transport ATPase)
Transmission and Scanning Electron Microscope Preparation of Whole Cultured Cells
促细胞分裂剂激活单核细胞
渗透(osmosis)
Hydrodynamic Analysis of Human Kinetochore Complexes During Mitosis
Studying Cell Cycle Checkpoints Using Drosophila Cultured Cells
Collagen Degradation Assays
Growing cells
NMR Studies of Three-Dimensional Structure and Positioning of CPPs in Membrane Model Systems
有丝分裂(mitosis)
Analysis of the Mammalian Cell Cycle by Flow Cytometry
DNA-Protein In Situ Covisualization for Chromosome Analysis
小麦与蚕豆的小孢子形成
Size-Selective and In Vitro Assessment of Inner Blood Retina Barrier Permeability
Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse
Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses
Identification and Separation of Normal Hematopoietic Stem Cells and Leukemia Stem Cells from Patien
Regulation of Focal Adhesion Dynamics by Wnt5a Signaling
Comparison of CPP Uptake Methods
人白细胞介素11抗体（IL-11 Ab）酶联免疫分析（ELISA）
Purification and Activation of Cyclic GMP-Dependent Protein Kinase
Crystallization of Membrane Proteins for X-Ray Analysis
人酶联免疫分析（ELISA）
Nature methods发布2013最受关注技术成果：单细胞测序
Analysis of Tissue-Specific DNA Methylation During Development
Isolation and Differentiation of Hair Follicle-Derived Dermal Precursors
Murine Aggregation Chimeras and Wholemount Imaging in Airway Stem Cell Biology
Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol
Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization
Culture of Human Pancreatic Islet Cells
通过优化由软件控制的加、减速率离心分离单核细胞
Human Embryonic Stem (ES) Cell Protocols――Matrigel Aliquoting and Plating
Conditional Gene Mutagenesis in B-Lineage Cells
Lentivirus-Mediated Modification of Pluripotent Stem Cells
Immobilization of Photosynthetic Membranes in an Albumin-Glutaraldehyde Crosslinked Matrix
Cryopreservation of Algae
Gene Targeting in Human Pluripotent Stem Cells
Analysis of Embryonic Vascular Morphogenesis
Assay of Protein Kinases and Protein Phosphorylation
The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Pr
The Isolation of Membrane Proteoglycans
Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases
Profiling Stem Cells Using Quantitative PCR Protein Assays
Evolution of the Wnt Pathways
Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent In Situ Hybridization
Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
Methods to Analyze Cellular Necroptosis
2,2,6,6-Tetramethylpiperidin-1-Oxyl Probes for Evaluating Oxidative Stress on the Cell Membrane and
Efficient Hepatic Differentiation from Human iPS Cells by Gene Transfer
Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg
Detection of Apoptosis in Mammalian Development
Differentiate ES cells into cystic embryoid bodies
Derivation of Human Parthenogenetic Stem Cell Lines
RAPD-PCR with Parasitic Hymenoptera
Screening for Radiation Sensitizers of Drosophila Checkpoint Mutants
Immobilization of Cells in Polyelectrolyte Complexes
Isolation of Fetal Gonads from Embryos of Timed-Pregnant Mice for Morphological and Molecular Studie
Identification of Osteoclasts in Culture
Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and
Glycol Methacrylate Embedding for Improved Morphological, Morphometrical, and Immunohistochemical In
Redox Regulation and Trapping Sulphenic Acid in the Peroxide Sensitive Human Mitochondrial Branched
Immune Properties of Mesenchymal Stem Cells
猴子白细胞介素12（IL-12）酶联免疫分析（ELISA）
透射电镜是如何实现超高分辨率的呢？
透射电镜与扫描电镜的区别
扫描电镜和透射电镜样品的要求有什么区别？
如何选择透射电镜实验设备？
透射电镜在生物技术中的应用
透射电镜常见问题解答
免疫荧光染色技术原理
免疫荧光染色步骤
免疫荧光染色实验方法
免疫荧光染色实验所需材料
免疫荧光染色封片技巧
免疫荧光染色到底有哪些目的呢？
免疫荧光染色与免疫组化的比较
免疫荧光染色与流式细胞仪的比较
免疫荧光染色实验仪器
免疫荧光染色常见问题以及解决方法
基因表达调控的基本原理
基因表达调控基本特点
如何学习基因表达调控？
基因表达调控方式有哪些？
基因表达受哪些因素调控？
基因表达调控在生物制药中的作用
转录因子与基因表达调控
基因表达正调控与负调控
基因表达调控技术有哪些？
基因表达调控教程
巨噬细胞分型基础
巨噬细胞及其多功能性详解
巨噬细胞分型m1m2区别
巨噬细胞分型研究方法
巨噬细胞系有哪些？
Topology Determination of Untagged Membrane Proteins
Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae
Identification and Quantitation of Ketamine in Biological Matrices Using Gas Chromatography-Mass Spe
Gastrulation and Early Mesodermal Patterning in Vertebrates
Monitoring MicroRNA Expression During Embryonic Stem-Cell Differentiation Using Quantitative Real-Ti
Corneal Assay for Angiogenesis
Selection of pH Ranges in 2DE
Transgenic Manipulation of the Sea Urchin Embryo
In Vitro Methods to Culture Primary Human Breast Epithelial Cells
Combined Immunofluorescence, RNA Fluorescent In Situ Hybridization, and DNA Fluorescent In Situ Hybr
Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks
Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture
Producing Soluble Recombinant RNases and Assays to Measure Their Interaction with Interferon- In Vit
兼性异染色质(facultative heterochromatin)
人鳞状上皮细胞癌抗原（SCCAg）酶联免疫分析（ELISA）
Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral Transduction
Epigenome Analysis of Pluripotent Stem Cells
细胞自发转化的若干问题与解决方案
Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation
Immunolabeling of Embryos
Coupling of Nuclear Localization Signals to Plasmid DNA
Ectopic Expression in Drosophila
Derivation and Maintenance of Embryonic Stem Cell Cultures
Use of a Hemacytometer
Derivation and Characterization of Neural Cells From Embryonic Stem Cells Using Nestin Enhancer
细胞培养基无机离子的作用
Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis
Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length
Determination of the Endothelial Barrier Function In Vitro
Introduction to Mass Spectrometry
Reporter Gene Technologies for Imaging Cell Fates in Hematopoiesis
Expression from DNA Injected into Xenopus Embryos
Crystal Violet Assay
Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems
Isolation and Purification of Rabbit Osteoclasts
Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Ba
Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery
Platelet Life Span and Apoptosis
Human Embryonic Stem (ES) Cell Protocols――Embryonic Bodies
Expression and Function of Claudins in Hepatocytes
High-Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes
巨噬细胞分型与免疫治疗
巨噬细胞分型意义
巨噬细胞分型的科研应用
RNA免疫沉淀（RIP）实验原理
RNA免疫沉淀（RIP）实验步骤流程详解
RNA免疫沉淀（RIP）实验数据分析
RNA免疫沉淀（RIP）实验材料的准备
RNA免疫沉淀（RIP）实验在生物学中的应用
RIP实验和pulldown的区别
RNA免疫沉淀（RIP）实验常见问题解答
RNA免疫沉淀（RIP）实验高级技巧
如何优化染色质免疫共沉淀（ChIP）实验条件？
ChIP实验常见问题解答
铁死亡
确保ChIP实验测序结果准确性的关键因素有哪些？
解释ChIP数据解读中需要注意的关键点
染色质免疫共沉淀（CHIP）实验原理详解
什么是ELISA实验？
ELISA实验步骤详解
ELISA实验数据分析与解读
Epithelial Cell Migration in Response to Epidermal Growth Factor
Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions
Exo Ovo Culture of Avian Embryos
Extraction of RNA from Fresh and Frozen Blood
Detection of Herpes Simplex Virus Dependent Apoptosis
The Design and Application of Ribosomal RNA-Targeted, Fluorescent Oligonucleotide Probes for the Ide
Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology
Biochemical Analyses of the Electron Transport Chain Complexes by Spectrophotometry
Flow Cytometric Analysis of Cell Cycle
Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functio
Analysis of Ligand-Dependent Nuclear Accumulation of Smads in TGF- Signaling
Human Embryonic Stem Cell Derivation, Maintenance, and Differentiation to Trophoblast
Overview: Assays for Studying Integrin-Dependent Cell Adhesion
Extraction and Assay of Cyclic Nucleotides
ELISA实验材料
引发体(primosome)
Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells
ELISA的实验报告（一）
In Vivo Imaging of Hematopoietic Stem Cells in the Bone Marrow Niche
Measurement of Mitochondrial dNTP Pools
ELISA检测实验报告（二）
Capabilities and Drawbacks of Phospholipid Analysis by MALDI-TOF Mass Spectrometry
The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability
Methods to Evaluate Alterations in Polyamine Metabolism Caused by Helicobacter pylori Infection
ELISA实验在生物医学研究中的应用案例
Experimental Approaches to the Study of Human Primordial Germ Cells
Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval
快速解读ELISA实验结果
Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells
ELISA实验中的创新技术——加速科研进程的新工具
ELISA试剂盒选择与使用指南
ELISA实验与Western Blot的异同点解析：如何选择更适合实验方法？
ELISA实验注意事项
ELISA实验常见问题解答
shRNA序列设计基因干扰原理
shRNA序列设计的具体步骤是什么？
探讨减少shRNA序列脱靶效应的方法
‌shRNA序列设计的关键因素
shRNA序列设计实战技巧：优化基因表达调控
介绍一些常用的shRNA表达载体
最新shRNA序列设计趋势与策略
构建shRNA序列设计流程
阐述siRNA和shRNA在细胞实验中的使用差异
分析siRNA和shRNA在细胞实验中可能遇到的问题及相应的解决方案
siRNA、shRNA 都是啥？
介绍提高shRNA转染效率的具体方法
解锁shRNA序列设计的秘密【提升实验成功率】
琼脂糖凝胶电泳步骤
提供预防琼脂糖凝胶中核酸降解的方法
琼脂糖凝胶电泳的上样量是多少？
Utility of Animal Models for Human Embryo Culture: Nonhuman Primates
琼脂糖凝胶电泳原理
Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs
Experimental Control and Characterization of Autophagy in Drosophila
COPⅠ被膜小泡(COPⅠcoated vesicle)
Homeoprotein Intercellular Transfer, the Hidden Face of Cell-Penetrating Peptides
关于琼脂糖凝胶电泳未出现条带的原因
Propagation of Human Embryonic Stem Cells on Human Feeder Cells
Methods for Imaging Cell Fates in Hematopoiesis
Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice
Multi-Dimensional Capillary Electrophoresis and Chromatography for Proteomic Analysis
Telomerase Inhibition by Synthetic Nucleic Acids and Chemosensitization in Human Bladder Cancer Cell
给出优化‌琼脂糖凝胶电泳结果分析‌的方法
The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions
探讨琼脂糖凝胶电泳在分子生物学研究中的发展趋势
Primary Human Osteoblast Cultures
细胞工程技术(cell engineering)
Silencing of Rho-GDI by RNAi Promotes the Differentiation of Neural Stem Cells
Induction of Cellular Senescence by Oncogenic RAS
Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer
In Vivo Matrigel Migration and Angiogenesis Assay
Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour of Cancer Cells In Vivo
The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery
Microinjection into Zebrafish Embryos
Descriptive Statistics
Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System
核孔复合物(nuclear pore complexs,NPCs)
Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry
Identification of Clock Genes Using Difference Gel Electrophoresis
Optimizing Stem Cell Grafting into the CNS
Analyzing Double-Strand Repair Events in Drosophila
微管(microtubule)
Novel Designed Probes for the Characterization of Oxidative Stress in Biological Fluids, Cells, and
High-Resolution Fluorescence In Situ Hybridization to Detect mRNAs in Neuronal Compartments In Vitro
Restriction Endonucleases and Their Uses
琼脂糖凝胶电泳试剂
琼脂糖凝胶电泳注意事项
亚细胞定位实验是什么？
亚细胞定位分析
详细描述GFP融合蛋白表达法的实验过程
亚细胞定位实验步骤
详细描述亚细胞定位实验的数据处理方式
列举几个影响亚细胞定位实验结果的关键因素
亚细胞定位实验结果解读
探讨如何优化亚细胞定位实验的设计以提高结果的准确性
对比分析不同亚细胞定位实验技术的优缺点
亚细胞定位实验设备
列举并分析亚细胞定位实验中的常见问题以及解决方案
巨噬细胞原代提取
‌巨噬细胞原代培养实验报告‌
巨噬细胞原代培养前后的区别
巨噬细胞m1m2标志物
详细描述巨噬细胞原代培养中细胞分离与培养的具体步骤
描述巨噬细胞原代培养中细胞贴壁的过程
探讨巨噬细胞原代培养中如何优化细胞活性
coip实验原理
coip实验流程以及步骤
coip实验设计
COIP实验步骤中，去除杂蛋白的具体方法是什么？
实验过程中，如何确保样品的低温处理？
coip实验数据解读
coip实验目的
coip实验后，如何分析实验结果?
分析CO-IP结果时，有哪些常见的误区需要避免？
如何优化CoIP实验以提高其准确性？
coip实验材料
‌coip实验应用
在设计COIP实验时，如何选择合适的抗体？
在进行COIP实验时，有哪些常见的问题和解决方法
CHIP引物设计原则
蛋白质翻译后修饰
RNA干扰技术
增强子及超级增强子序列查找
原核表达载体和真核表达系统及其载体
分子生物学导论
ChIP实验中引物序列的选择策略
在CHIP实验中，如何确保引物的特异性和有效性？
CHIP引物设计步骤
CHIP引物设计技巧
CHIP引物设计与基因表达的研究
CHIP实验引物要求‌
CHIP引物设计结果分析
在设计CHIP引物时，如何确保引物的扩增效率？
CHIP引物设计常见问题
CHIP引物设计教程
Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in
Quantitation of 17-OH-Progesterone (OHPG) for Diagnosis of Congenital Adrenal Hyperplasia (CAH)
Detection of Aromatic -Hydroxyketones with Tetrazolium Salts
Random Mutagenesis of Short Target DNA Sequences via PCR with Degenerate Oligonucleotides
Analysis of Nuclear UracilDNA Glycosylase (nUDG) Turnover During the Cell Cycle
Detection of Cell Death in Drosophila
Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpole
Adult Stem Cells for the Treatment of Neurological Disease
Long-Circulating, pH-Sensitive Liposomes
Detection of Nuclear -catenin in Xenopus Embryos
Zymographical Techniques for Detectionof Matrix Metalloproteinases
Aging Studies in Drosophila Melanogaster
The Mitochondria of Cultured Mammalian Cells: I: Analysis by Immunofluorescence Microscopy, Histoche
Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies
Use of Divalent Metal Ions Chelated to Agarose Derivatives for Reversible Immobilization of Proteins
Analysis of EGF Receptor Interactions With the Subunit of the Stimulatory GTP Binding Protein of Ade
Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of
Analysing the ATP Turnover Cycle of Microtubule Motors
Isolation of mRNAs Encoding Peroxisomal Proteins from Yeast Using a Combined Cell Fractionation and
Isolation, Characterization, and Reaggregate Culture of Thymic Epithelial Cells
Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo
Application of Cre/loxP in Drosophila: Site-Specific Recombination and Transgene Coplacement
Isolation and Culture of Adult Mouse Hepatocytes
Expression of Recombinant ADAMTS in Insect Cells
Analysis of Microtubule Assembly Kinetics Using Turbidimetry
Hematopoietic Progenitor Cell Collection
Direct Sequencing with Highly Degenerate and lnosine-Containing Primers
Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities
培养物的污染及防止
The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its
Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell T
Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress
体外肝细胞损伤模型建立（CCl4和H2O2）
Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells
In Vitro Assays for Neural Stem Cell Differentiation
Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitt
A Transplant Model for Human Epidermal Skin Regeneration
Real-Time RT-PCR for CTG Repeat-Containing Genes
Isolation of Total RNA from Bacteria
The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells
支原体污染后的治疗和处理
Quantitating mRNAs with Relative and Competitive RT-PCR
Reverse Transfected Cell Microarrays in Infectious Disease Research
Copy Number Determination of Genetically-Modified Hematopoietic Stem Cells
High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays
CHIP引物设计四个注意事项详细点
细胞铁死亡的定义
细胞铁死亡的机制
细胞铁死亡造模流程
细胞铁死亡信号通路
细胞铁死亡金标准
细胞铁死亡研究与应用整合
细胞铁死亡实验方法
细胞铁死亡和凋亡的区别
入门细胞铁死亡研究，这些基础知识你都知道吗?
非编码RNA作用原理是什么？
非编码RNA定义
非编码RNA在细胞分裂中有什么作用？
非编码RNA种类和功能
长非编码RNA如何参与基因转录调控？
非编码RNA研究的主要方向是什么？
非编码RNA和编码RNA区别
非编码RNA由什么转录？
Routine Culture and Differentiation of Human Embryonic Stem Cells
An Immunoprecipitation-RNA:rPCR Method for the In Vivo Isolation of Ribonucleoprotein Complexes
非编码RNA的实验室检测技术
Under-Agarose Chemotaxis of Dictyostelium discoideum
加州大学研究人员开发出了“智能培养基
Genetic-Engineered Models of Skeletal Diseases I: Collagen Type X
非编码RNA特点
Analysis by HPLC of Distributive Activities and the Synthetic (Back) Reaction of Pancreatic-Type Rib
Tissue Culture - Thawing Cells from Liquid Nitrogen
细胞自噬大盘点
In Vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth Characteristics
Keratinocyte Culture
细胞自噬机制
BacterialBacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins
Optimized Neuronal Differentiation of Murine Embryonic Stem Cells: Role of Cell Density
细胞自噬与肿瘤的关系
RC4DRestriction Fragment Length Polymorphism-Coupled Domain-Directed Differential Display
Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue
Senescence Regulation by mTOR
GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications
细胞自噬与免疫调节
Analyzing the Angiogenic Potential of Gr-1+CD11b+ Immature Myeloid Cells from Murine Wounds
Optimizing Sporulation Conditions for Different Saccharomyces cerevisiae Strain Backgrounds
细胞自噬与衰老
The Use of Synthetic DNA Probes for the Field Identification of Members of the Anopheles gambiae Com
细胞自噬：从基础到临床，生命科学的深度解析
Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS
Electrofusion of Mouse Embryos to Produce Tetraploids
Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development
多线染色体(polytene chromosome)
Immunofluorescence of Cultured Cells
DNA Damage Response and Mutagenesis in Mouse Embryonic Stem Cells
Tissue Procurement for Molecular Studies Using Laser-Assisted Microdissection

汽水

最大持续产量（maximum sustained yield）

水的生物净化

蛋白质生物合成的原料

晶格

类别异构

武汉专家利用油菜籽渣炼出生物柴油

辅酶（coenzyme）

巴西呼吁进行非洲生物燃料“革命”

果糖 （fructose）

自主序列复制系统

野生大麦(wild barley)

转录依赖的扩增系统

猪生长激素（GH）酶联免疫分析

猪干扰素-γ（IFN-γ）酶联免疫分析（ELISA）

猪传染性胸膜肺炎 (PCP)酶联免疫分析（ELISA）

猪钙调神经磷酸酶(CaN)酶联免疫分析

猪表皮生长因子(EGF)酶联免疫分析（ELISA）

重水

中子

白垩纪（Cretaceous Period，Cretaceous）

赣将建国内首个生物柴油用于车辆行驶项目

海棠脯

质子

香水梨干

鳗鱼火腿

酒心巧克力

红烧鲐鱼

茄汁沙丁鱼罐头

凤尾鱼罐头

茄汁黄鱼

精制巧克力

糖醋瓜缨

进化速度（speed of evolution）

生物元素

植物吲哚乙酸（IAA）酶联免疫分析（ELISA）

层片（synusia）

植物脱落酸(ABA)酶联免疫分析（ELISA）

植物钙调磷酸酶（CaN）酶联免疫分析（ELISA）

植物超氧化物歧化酶（SOD）酶联免疫分析（ELISA）

菠萝果冻

糖桔饼

猕猴桃酸奶

人体气味的色谱分析

蛋白复性（三）-蛋白质的结构转换

RNA操作中小结

海洋牧业—一蓝色革命

臭氧层空洞

离子的极化

鲜味果酱

气体摩尔体积

光谱分析——原子吸收分光光度法

玉米秸饴糖

杏仁牛奶冰霜

桔味棒冰

胡桃冰砖

栗子冰砖

玫瑰杨梅

菠萝酱

山楂饼

仙桃酱

黑加仑子果酱

槟子酱

麻酥糖(上海)

鱼松

鱼松(江南风味)

咸味杨梅

果香醉枣

杨梅干(苏式)

家制桃脯

牛奶苹果泥酥

香草话梅(苏式)

玫瑰蜜饯(川式)

梨子蜜饯(川式)

桂花蜜饯(川式)

去皮酥(福式蜜饯)

蜜橙皮(川式)

西瓜冻

西瓜皮糖酱

香草桃片(广式)

果糖（fructose）